                         SEQUENCE LISTING

<110>  Pieris AG
 
<120>  Muteins of human lipocalin 2 with affinity for Glypican-3 (GPC3)

<130>  PIE15296PCT


<150>  EP15167922.2
<151>  2015-05-18

<160>  30    

<170>  PatentIn version 3.5

<210>  1
<211>  178
<212>  PRT
<213>  artificial

<220>
<223>  Wildtype NGAL

<400>  1

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe Gln Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Leu Ala Gly Asn Ala Ile Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              


Gln Lys Met Tyr Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  


Asn Val Thr Ser Val Leu Phe Arg Lys Lys Lys Cys Asp Tyr Trp Ile 
65                  70                  75                  80  


Arg Thr Phe Val Pro Gly Cys Gln Pro Gly Glu Phe Thr Leu Gly Asn 
                85                  90                  95      


Ile Lys Ser Tyr Pro Gly Leu Thr Ser Tyr Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Ser Gln 
        115                 120                 125             


Asn Arg Glu Tyr Phe Lys Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 


Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 


Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     


Asp Gly 
        


<210>  2
<211>  178
<212>  PRT
<213>  artificial

<220>
<223>  Wildtype NGAL98

<400>  2

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Leu Ala Gly Asn Ala Ile Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              


Gln Lys Met Tyr Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  


Asn Val Thr Ser Val Leu Phe Arg Lys Lys Lys Cys Asp Tyr Trp Ile 
65                  70                  75                  80  


Arg Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 
                85                  90                  95      


Ile Lys Ser Tyr Pro Gly Leu Thr Ser Tyr Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Ser Gln 
        115                 120                 125             


Asn Arg Glu Tyr Phe Lys Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 


Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 


Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     


Asp Gly 
        


<210>  3
<211>  178
<212>  PRT
<213>  artificial

<220>
<223>  Lipocalin mutein - parental NGAL (P019-Seq2 - S375.1 M1.1 A16)

<400>  3

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Val Ala Gly Asn Ala Met Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              


Leu Lys Met Arg Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  


Asn Val Thr Gly Val Ser Phe Trp Arg Lys Lys Cys His Tyr Lys Ile 
65                  70                  75                  80  


Gly Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asp 
                85                  90                  95      


Ile Lys Ser Gly Pro Gly Gln Thr Ser Asn Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Glu Val Arg Gln 
        115                 120                 125             


Asn Arg Glu Trp Phe Ala Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 


Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 


Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     


Asp Gly 
        


<210>  4
<211>  178
<212>  PRT
<213>  artificial

<220>
<223>  Lipocalin mutein - parental NGAL (P019-Seq 3 - S375.2 M1.1 M2)

<400>  4

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Arg Ala Gly Asn Val Ala Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              


Pro Lys Met Arg Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  


Asn Val Thr Asn Val Arg Phe Ala Met Lys Lys Cys Met Tyr Ser Ile 
65                  70                  75                  80  


Gly Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Gln 
                85                  90                  95      


Ile Lys Ser Glu Pro Gly Asn Thr Ser Asn Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Glu Val Tyr Gln 
        115                 120                 125             


Asn Arg Glu Ile Phe Phe Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 


Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 


Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     


Asp Gly 
        


<210>  5
<211>  178
<212>  PRT
<213>  artificial

<220>
<223>  Lipocalin mutein (S0569.05N06M1)

<400>  5

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe Gln Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Val Ala Gly Asn Ala Leu Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              


Leu Lys Met Arg Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  


Asp Val Thr Val Val Ser Phe Trp Arg Lys Lys Cys His Tyr Lys Ile 
65                  70                  75                  80  


Gly Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asp 
                85                  90                  95      


Ile Lys Ser Gly Pro Gly Gln Thr Ser Asn Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Glu Val Arg Gln 
        115                 120                 125             


Asn Arg Glu Trp Phe Ala Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 


Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 


Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     


Asp Gly 
        


<210>  6
<211>  178
<212>  PRT
<213>  artificial

<220>
<223>  Lipocalin mutein (S0569.05K19M1)

<400>  6

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe Gln Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Val Ala Gly Asn Val Arg Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              


Pro Lys Met Arg Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  


Asp Val Thr Gly Val Ser Phe Arg Gly Lys Lys Cys His Tyr Lys Ile 
65                  70                  75                  80  


Gly Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asp 
                85                  90                  95      


Ile Lys Ser Gly Pro Gly Glu Thr Ser Asn Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Glu Val Arg Gln 
        115                 120                 125             


Asn Arg Glu Trp Phe Phe Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 


Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 


Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     


Asp Gly 
        


<210>  7
<211>  178
<212>  PRT
<213>  artificial

<220>
<223>  Lipocalin mutein (S0569.05O04M1)

<400>  7

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe Gln Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Val Ala Gly Asn Gly Met Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              


Leu Lys Met Arg Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  


Asp Val Thr Ser Val Ala Phe Arg Asn Lys Lys Cys His Tyr Lys Ile 
65                  70                  75                  80  


Gly Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Gln 
                85                  90                  95      


Ile Lys Ser Gly Pro Gly Glu Thr Ser Asn Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Glu Val Arg Gln 
        115                 120                 125             


Asn Arg Glu Trp Phe Phe Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 


Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 


Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     


Asp Gly 
        


<210>  8
<211>  178
<212>  PRT
<213>  artificial

<220>
<223>  Lipocalin mutein (S0569.02J22M1)

<400>  8

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe Gln Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Arg Ala Gly Asn Val Gly Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              


Pro Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  


Asp Val Thr Asn Val Arg Phe Ala Arg Lys Lys Cys Leu Tyr Ser Ile 
65                  70                  75                  80  


Gly Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Gln 
                85                  90                  95      


Ile Lys Ser Glu Pro Gly Asn Thr Ala Asn Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Glu Val Tyr Gln 
        115                 120                 125             


Asn Arg Glu Ile Phe Phe Ile Ile Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 


Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 


Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     


Asp Gly 
        


<210>  9
<211>  178
<212>  PRT
<213>  artificial

<220>
<223>  Lipocalin mutein (S0569.02B06M1)

<400>  9

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe Gln Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Arg Ala Gly Asn Val Gly Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              


Pro Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  


Asp Val Thr Asn Val Arg Phe Ala Arg Lys Lys Cys Thr Tyr Ser Ile 
65                  70                  75                  80  


Gly Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Gln 
                85                  90                  95      


Ile Lys Ser Glu Pro Gly Gly Thr Ala Asn Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Glu Val Tyr Gln 
        115                 120                 125             


Asn Arg Glu Ile Phe Phe Ile Ile Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 


Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 


Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     


Asp Gly 
        


<210>  10
<211>  178
<212>  PRT
<213>  artificial

<220>
<223>  Lipocalin mutein (S0569.02L21M1)

<400>  10

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe Gln Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Arg Ala Gly Asn Gly Ala Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              


Pro Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  


Asp Val Thr Asn Val Arg Phe Ala Arg Lys Lys Cys Val Tyr Ser Ile 
65                  70                  75                  80  


Gly Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Pro 
                85                  90                  95      


Ile Lys Ser Glu Pro Gly Asn Thr Ala Ser Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Glu Val Tyr Gln 
        115                 120                 125             


Asn Arg Glu Ile Phe Phe Ile Ile Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 


Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 


Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     


Asp Gly 
        


<210>  11
<211>  178
<212>  PRT
<213>  artificial

<220>
<223>  Lipocalin mutein (S0569.02A11M1)

<400>  11

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe Gln Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Arg Ala Gly Asn Val Ala Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              


Pro Lys Met Arg Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  


Asp Val Thr Ala Val Arg Phe Ala Arg Lys Lys Cys Leu Tyr Ser Ile 
65                  70                  75                  80  


Gly Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Arg 
                85                  90                  95      


Ile Lys Ser Glu Pro Gly Tyr Thr Ala Asn Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Glu Val Tyr Gln 
        115                 120                 125             


Asn Arg Glu Ile Phe Phe Ile Ile Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 


Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 


Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     


Asp Gly 
        


<210>  12
<211>  178
<212>  PRT
<213>  artificial

<220>
<223>  Lipocalin mutein (S0569.09P24M1)

<400>  12

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe Gln Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Arg Ala Gly Asn Val Ala Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              


Pro Lys Met Arg Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  


Asp Val Thr Asn Val Val Phe Ala Gly Lys Lys Cys Lys Tyr Ser Ile 
65                  70                  75                  80  


Gly Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Arg 
                85                  90                  95      


Ile Lys Ser Pro Pro Gly Asn Thr Ala Asn Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Glu Val Tyr Gln 
        115                 120                 125             


Asn Arg Glu Ile Phe Phe Ile Ile Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 


Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 


Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     


Asp Gly 
        


<210>  13
<211>  178
<212>  PRT
<213>  artificial

<220>
<223>  Lipocalin mutein (S0569.02N03M1)

<400>  13

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe Gln Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Arg Ala Gly Asn Leu Gly Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              


Pro Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  


Asp Val Thr Asn Val Arg Phe Ala Arg Lys Lys Cys Met Tyr Ser Ile 
65                  70                  75                  80  


Gly Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Gln 
                85                  90                  95      


Ile Lys Ser Glu Pro Gly Ser Thr Ala Asn Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Glu Val Tyr Gln 
        115                 120                 125             


Asn Arg Glu Ile Phe Phe Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 


Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 


Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     


Asp Gly 
        


<210>  14
<211>  178
<212>  PRT
<213>  artificial

<220>
<223>  Lipocalin mutein (S0569.10I10M1)

<400>  14

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe Gln Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Arg Ala Gly Asn Val Gly Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              


Pro Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  


Asp Val Thr Asn Val Arg Phe Ala Gly Lys Lys Val Lys Tyr Thr Ile 
65                  70                  75                  80  


Gly Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Gln 
                85                  90                  95      


Ile Lys Ser Glu Pro Gly Asn Thr Ala Thr Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Glu Val Tyr Gln 
        115                 120                 125             


Asn Arg Glu Ile Phe Phe Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 


Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 


Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Ala Ile 
                165                 170                 175     


Asp Gly 
        


<210>  15
<211>  178
<212>  PRT
<213>  artificial

<220>
<223>  Lipocalin mutein (S0569.10D09M1)

<400>  15

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe Gln Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Arg Ala Gly Asn Val Gly Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              


Pro Lys Met Arg Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  


Asp Val Thr Gly Val Arg Phe Gly Glu Lys Lys Ile Lys Tyr Ser Ile 
65                  70                  75                  80  


Gly Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Gln 
                85                  90                  95      


Ile Lys Ser Gln Pro Gly Asp Thr Ala Asn Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Glu Val Tyr Gln 
        115                 120                 125             


Asn Arg Glu Ile Phe Phe Ile Ile Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 


Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 


Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Ala Ile 
                165                 170                 175     


Asp Gly 
        


<210>  16
<211>  178
<212>  PRT
<213>  artificial

<220>
<223>  Lipocalin mutein (S0569.04M18M2)

<400>  16

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe Gln Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Arg Ala Gly Asn Val Gly Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              


Pro Lys Met Arg Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  


Asp Val Thr Gly Val Arg Phe Asp Ser Lys Lys Val Thr Tyr Ser Ile 
65                  70                  75                  80  


Gly Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Gln 
                85                  90                  95      


Ile Lys Ser Glu Pro Gly Asn Thr Ala Asn Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Glu Val Tyr Gln 
        115                 120                 125             


Asn Arg Glu Ile Phe Phe Ile Ile Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 


Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 


Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Ala Ile 
                165                 170                 175     


Asp Gly 
        


<210>  17
<211>  10
<212>  PRT
<213>  artificial

<220>
<223>  SA linker and Strep-tag II

<400>  17

Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
1               5                   10  


<210>  18
<211>  8
<212>  PRT
<213>  artificial

<220>
<223>  Strep-tag II

<400>  18

Trp Ser His Pro Gln Phe Glu Lys 
1               5               


<210>  19
<211>  534
<212>  DNA
<213>  artificial

<220>
<223>  Lipocalin mutein (S0569.05N06M1)

<400>  19
caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag       60

aacttccagg acaaccaatt ccaagggaaa tggtatgtcg tgggcgttgc cggaaatgct      120

ctgctgcgtg aggataagga tccgcttaaa atgagggcga ccatttacga gttgaaagaa      180

gataaatcat atgacgtcac cgttgtgtct ttttggagga agaaatgcca ttacaagatt      240

gggacctttg tgccggggag ccagccgggc gagtttactt taggcgatat taaaagtggg      300

ccgggccaga catcaaattt ggtccgcgtc gtgagcacca actacaacca gcatgccatg      360

gtgttcttca aggaggtgag gcagaaccgc gagtggtttg ctatcacact gtacgggcgc      420

acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc      480

ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc            534


<210>  20
<211>  534
<212>  DNA
<213>  artificial

<220>
<223>  Lipocalin mutein (S0569.05K19M1)

<400>  20
caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag       60

aacttccagg acaaccaatt ccaagggaaa tggtatgtcg tgggcgttgc cggaaatgtt      120

cgtctgcgtg aggataagga tccgcctaaa atgagggcga ccatttacga gttgaaagaa      180

gataaatcat atgacgtcac cggtgtgtct tttcggggga agaaatgcca ttacaagatt      240

gggacctttg tgccggggag ccagccgggc gagtttactt taggcgatat taaaagtggt      300

ccgggcgaga catcaaattt ggtccgcgtc gtgagcacca actacaacca gcatgccatg      360

gtgttcttca aggaggtgag gcagaaccgc gagtggtttt ttatcacact gtacgggcgc      420

acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc      480

ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc            534


<210>  21
<211>  534
<212>  DNA
<213>  artificial

<220>
<223>  Lipocalin mutein (S0569.05O04M1)

<400>  21
caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag       60

aacttccagg acaaccaatt ccaagggaaa tggtatgtcg tgggcgttgc cggaaatggt      120

atgctgcgtg aggataagga tccgcttaaa atgagggcga ccatttacga gttgaaagaa      180

gataaatcat atgacgtcac cagtgtggct tttcggaata agaaatgcca ttacaagatt      240

gggacctttg tgccggggag ccagccgggc gagtttactt taggccagat taaaagtggt      300

ccgggcgaga catcaaattt ggtccgcgtc gtgagcacca actacaacca gcatgccatg      360

gtgttcttca aggaggtgag gcagaaccgc gagtggtttt ttatcacact gtacgggcgc      420

acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc      480

ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc            534


<210>  22
<211>  534
<212>  DNA
<213>  artificial

<220>
<223>  Lipocalin mutein (S0569.02J22M1)

<400>  22
caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag       60

aacttccagg acaaccaatt ccaagggaaa tggtatgtcg tgggcagggc cggaaatgtg      120

gggctgcgtg aggataagga tccgcctaaa atgtgggcga ccatttacga gttgaaagaa      180

gataaatcat atgacgtcac caatgtgagg tttgctagga agaaatgctt gtactcgatt      240

gggacctttg tgccggggag ccagccgggc gagtttactt taggccagat taaaagtgag      300

ccgggcaata cagcaaattt ggtccgcgtc gtgagcacca actacaacca gcatgccatg      360

gtgttcttca aggaggtgta tcagaaccgc gagatttttt ttatcatact gtacgggcgc      420

acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc      480

ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc            534


<210>  23
<211>  534
<212>  DNA
<213>  artificial

<220>
<223>  Lipocalin mutein (S0569.02B06M1)

<400>  23
caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag       60

aacttccagg acaaccaatt ccaagggaaa tggtatgtcg tgggcagggc cggaaatgtg      120

ggtctgcgtg aggataagga tccgcctaaa atgtgggcga ccatttacga gttgaaagaa      180

gataaatcat atgacgtcac caatgtgagg tttgctagga agaaatgcac gtactcgatt      240

gggacctttg tgccggggag ccagccgggc gagtttactt taggccagat taaaagtgag      300

ccgggcggta cagcaaattt ggtccgcgtc gtgagcacca actacaacca gcatgccatg      360

gtgttcttca aggaggtgta tcagaaccgc gagatttttt ttatcatact gtacgggcgc      420

acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc      480

ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc            534


<210>  24
<211>  534
<212>  DNA
<213>  artificial

<220>
<223>  Lipocalin mutein (S0569.02L21M1)

<400>  24
caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag       60

aacttccagg acaaccaatt ccaagggaaa tggtatgtcg tgggcagggc cggaaatggg      120

gctctgcgtg aggataagga tccgcctaaa atgtgggcga ccatttacga gttgaaagaa      180

gataaatcat atgacgtcac caatgtgagg tttgctagga agaaatgcgt gtactcgatt      240

gggacctttg tgccggggag ccagccgggc gagtttactt taggcccgat taaaagtgag      300

ccgggcaata cagcatcttt ggtccgcgtc gtgagcacca actacaacca gcatgccatg      360

gtgttcttca aggaggtgta tcagaaccgc gagatttttt ttatcatact gtacgggcgc      420

acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc      480

ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc            534


<210>  25
<211>  534
<212>  DNA
<213>  artificial

<220>
<223>  Lipocalin mutein (S0569.02A11M1)

<400>  25
caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag       60

aacttccagg acaaccaatt ccaagggaaa tggtatgtcg tgggcagggc cggaaatgtt      120

gctctgcgtg aggataagga tccgcctaaa atgagggcga ccatttacga gttgaaagaa      180

gataaatcat atgacgtcac cgctgtgagg tttgctagga agaaatgcct gtactcgatt      240

gggacctttg tgccggggag ccagccgggc gagtttactt taggccggat taaaagtgag      300

ccgggctata cagcaaattt ggtccgcgtc gtgagcacca actacaacca gcatgccatg      360

gtgttcttca aggaggtgta tcagaaccgc gagatttttt ttatcatact gtacgggcgc      420

acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc      480

ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc            534


<210>  26
<211>  534
<212>  DNA
<213>  artificial

<220>
<223>  Lipocalin mutein (S0569.09P24M1)

<400>  26
caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag       60

aacttccagg acaaccaatt ccaagggaaa tggtatgtcg tgggcagggc cggaaatgtg      120

gctctgcgtg aggataagga tccgcctaaa atgagggcga ccatttacga gttgaaagaa      180

gataaatcat atgacgtcac caatgtggtg tttgctggga agaaatgcaa gtactcgatt      240

gggacctttg tgccggggag ccagccgggc gagtttactt taggccggat taaaagtccg      300

ccgggcaata cagcaaattt ggtccgcgtc gtgagcacca actacaacca gcatgccatg      360

gtgttcttca aggaggtgta tcagaaccgc gagatttttt ttatcatact gtacgggcgc      420

acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc      480

ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc            534


<210>  27
<211>  534
<212>  DNA
<213>  artificial

<220>
<223>  Lipocalin mutein (S0569.02N03M1)

<400>  27
caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag       60

aacttccagg acaaccaatt ccaagggaaa tggtatgtcg tgggcagggc cggaaatctg      120

ggtctgcgtg aggataagga tccgcctaaa atgtgggcga ccatttacga gttgaaagaa      180

gataaatcat atgacgtcac caatgtgagg tttgctagga agaaatgcat gtactctatt      240

ggtacctttg tgccggggag ccagccgggc gagtttactt taggccagat taaaagtgag      300

ccgggcagta cagcaaattt ggtccgcgtc gtgagcacca actacaacca gcatgccatg      360

gtgttcttca aggaggtgta tcagaaccgc gagatttttt ttatcacact gtacgggcgc      420

acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc      480

ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc            534


<210>  28
<211>  534
<212>  DNA
<213>  artificial

<220>
<223>  Lipocalin mutein (S0569.10I10M1)

<400>  28
caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag       60

aacttccagg acaaccaatt ccaagggaaa tggtatgtcg tgggcagggc cggaaatgtg      120

ggtctgcgtg aggataagga tccgcctaaa atgtgggcga ccatttacga gttgaaagaa      180

gataaatcat atgacgtcac caatgtgagg tttgctggga agaaagttaa gtacacgatt      240

gggacctttg tgccggggag ccagccgggc gagtttactt taggccagat taaaagtgag      300

ccgggcaata cagcaacttt ggtccgcgtc gtgagcacca actacaacca gcatgccatg      360

gtgttcttca aggaggtgta tcagaaccgc gagatttttt ttatcacact gtacgggcgc      420

acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc      480

ctccctgaaa accacatcgt cttccctgtc ccaatcgacc aggctatcga cggc            534


<210>  29
<211>  534
<212>  DNA
<213>  artificial

<220>
<223>  Lipocalin mutein (S0569.10D09M1)

<400>  29
caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag       60

aacttccagg acaaccaatt ccaagggaaa tggtatgtcg tgggcagggc cggaaatgtg      120

ggtctgcgtg aggataagga tccgcctaaa atgagggcga ccatttacga gttgaaagaa      180

gataaatcat atgacgtcac cggtgtgagg tttggtgaga agaaaattaa gtactcgatt      240

ggtacctttg tgccggggag ccagccgggc gagtttactt taggccagat taaaagtcag      300

ccgggcgata cagcaaattt ggtccgcgtc gtgagcacca actacaacca gcatgccatg      360

gtgttcttca aggaggtgta tcagaaccgc gagatttttt ttatcatact gtacgggcgc      420

acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc      480

ctccctgaaa accacatcgt cttccctgtc ccaatcgacc aggctatcga cggc            534


<210>  30
<211>  534
<212>  DNA
<213>  artificial

<220>
<223>  Lipocalin mutein (S0569.04M18M2)

<400>  30
caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag       60

aacttccagg acaaccaatt ccaagggaaa tggtatgtcg tgggccgggc cggaaatgtg      120

ggtctgcgtg aggataagga tccgcctaaa atgagggcga ccatttacga gttgaaagaa      180

gataaatcat atgacgtcac cggtgtgagg tttgattcta agaaagtgac gtactctatt      240

gggacctttg tgccggggag ccagccgggc gagtttactt taggccagat taaaagtgag      300

ccgggcaata cagcgaattt ggtccgcgtc gtgagcacca actacaacca gcatgccatg      360

gtgttcttca aggaggtgta tcagaaccgc gagatttttt ttatcatact gtacgggcgc      420

acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc      480

ctccctgaaa accacatcgt cttccctgtc ccaatcgacc aggctatcga cggc            534


