                         SEQUENCE LISTING

<110>  WALLECHA, Anu
       MOLLI, Poonam
       PETIT, Robert
 
<120>  IMMUNOGENIC LISTERIA-BASED COMPOSITIONS COMPRISING TRUNCATED 
       ACTA-ANTIGEN FUSIONS AND METHODS OF USE THEREOF

<130>  62384-479248

<140>  US 62/160,764
<141>  2015-05-13

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  14
<212>  PRT
<213>  Listeria monocytogenes

<400>  1

Lys Thr Glu Glu Gln Pro Ser Glu Val Asn Thr Gly Pro Arg 
1               5                   10                  


<210>  2
<211>  28
<212>  PRT
<213>  Listeria monocytogenes

<400>  2

Lys Glu Ser Val Val Asp Ala Ser Glu Ser Asp Leu Asp Ser Ser Met 
1               5                   10                  15      


Gln Ser Ala Asp Glu Ser Thr Pro Gln Pro Leu Lys 
            20                  25              


<210>  3
<211>  20
<212>  PRT
<213>  Listeria monocytogenes

<400>  3

Lys Ser Glu Glu Val Asn Ala Ser Asp Phe Pro Pro Pro Pro Thr Asp 
1               5                   10                  15      


Glu Glu Leu Arg 
            20  


<210>  4
<211>  33
<212>  PRT
<213>  Listeria monocytogenes

<400>  4

Arg Gly Gly Ile Pro Thr Ser Glu Glu Phe Ser Ser Leu Asn Ser Gly 
1               5                   10                  15      


Asp Phe Thr Asp Asp Glu Asn Ser Glu Thr Thr Glu Glu Glu Ile Asp 
            20                  25                  30          


Arg 
    


<210>  5
<211>  17
<212>  PRT
<213>  Streptococcus pyogenes

<400>  5

Lys Gln Asn Thr Ala Ser Thr Glu Thr Thr Thr Thr Asn Glu Gln Pro 
1               5                   10                  15      


Lys 
    


<210>  6
<211>  17
<212>  PRT
<213>  Streptococcus equisimilis

<400>  6

Lys Gln Asn Thr Ala Asn Thr Glu Thr Thr Thr Thr Asn Glu Gln Pro 
1               5                   10                  15      


Lys 
    


<210>  7
<211>  633
<212>  PRT
<213>  Listeria monocytogenes

<400>  7

Met Arg Ala Met Met Val Val Phe Ile Thr Ala Asn Cys Ile Thr Ile 
1               5                   10                  15      


Asn Pro Asp Ile Ile Phe Ala Ala Thr Asp Ser Glu Asp Ser Ser Leu 
            20                  25                  30          


Asn Thr Asp Glu Trp Glu Glu Glu Lys Thr Glu Glu Gln Pro Ser Glu 
        35                  40                  45              


Val Asn Thr Gly Pro Arg Tyr Glu Thr Ala Arg Glu Val Ser Ser Arg 
    50                  55                  60                  


Asp Ile Glu Glu Leu Glu Lys Ser Asn Lys Val Lys Asn Thr Asn Lys 
65                  70                  75                  80  


Ala Asp Leu Ile Ala Met Leu Lys Ala Lys Ala Glu Lys Gly Pro Asn 
                85                  90                  95      


Asn Asn Asn Asn Asn Gly Glu Gln Thr Gly Asn Val Ala Ile Asn Glu 
            100                 105                 110         


Glu Ala Ser Gly Val Asp Arg Pro Thr Leu Gln Val Glu Arg Arg His 
        115                 120                 125             


Pro Gly Leu Ser Ser Asp Ser Ala Ala Glu Ile Lys Lys Arg Arg Lys 
    130                 135                 140                 


Ala Ile Ala Ser Ser Asp Ser Glu Leu Glu Ser Leu Thr Tyr Pro Asp 
145                 150                 155                 160 


Lys Pro Thr Lys Ala Asn Lys Arg Lys Val Ala Lys Glu Ser Val Val 
                165                 170                 175     


Asp Ala Ser Glu Ser Asp Leu Asp Ser Ser Met Gln Ser Ala Asp Glu 
            180                 185                 190         


Ser Thr Pro Gln Pro Leu Lys Ala Asn Gln Lys Pro Phe Phe Pro Lys 
        195                 200                 205             


Val Phe Lys Lys Ile Lys Asp Ala Gly Lys Trp Val Arg Asp Lys Ile 
    210                 215                 220                 


Asp Glu Asn Pro Glu Val Lys Lys Ala Ile Val Asp Lys Ser Ala Gly 
225                 230                 235                 240 


Leu Ile Asp Gln Leu Leu Thr Lys Lys Lys Ser Glu Glu Val Asn Ala 
                245                 250                 255     


Ser Asp Phe Pro Pro Pro Pro Thr Asp Glu Glu Leu Arg Leu Ala Leu 
            260                 265                 270         


Pro Glu Thr Pro Met Leu Leu Gly Phe Asn Ala Pro Thr Pro Ser Glu 
        275                 280                 285             


Pro Ser Ser Phe Glu Phe Pro Pro Pro Pro Thr Asp Glu Glu Leu Arg 
    290                 295                 300                 


Leu Ala Leu Pro Glu Thr Pro Met Leu Leu Gly Phe Asn Ala Pro Ala 
305                 310                 315                 320 


Thr Ser Glu Pro Ser Ser Phe Glu Phe Pro Pro Pro Pro Thr Glu Asp 
                325                 330                 335     


Glu Leu Glu Ile Met Arg Glu Thr Ala Pro Ser Leu Asp Ser Ser Phe 
            340                 345                 350         


Thr Ser Gly Asp Leu Ala Ser Leu Arg Ser Ala Ile Asn Arg His Ser 
        355                 360                 365             


Glu Asn Phe Ser Asp Phe Pro Leu Ile Pro Thr Glu Glu Glu Leu Asn 
    370                 375                 380                 


Gly Arg Gly Gly Arg Pro Thr Ser Glu Glu Phe Ser Ser Leu Asn Ser 
385                 390                 395                 400 


Gly Asp Phe Thr Asp Asp Glu Asn Ser Glu Thr Thr Glu Glu Glu Ile 
                405                 410                 415     


Asp Arg Leu Ala Asp Leu Arg Asp Arg Gly Thr Gly Lys His Ser Arg 
            420                 425                 430         


Asn Ala Gly Phe Leu Pro Leu Asn Pro Phe Ile Ser Ser Pro Val Pro 
        435                 440                 445             


Ser Leu Thr Pro Lys Val Pro Lys Ile Ser Ala Pro Ala Leu Ile Ser 
    450                 455                 460                 


Asp Ile Thr Lys Lys Ala Pro Phe Lys Asn Pro Ser Gln Pro Leu Asn 
465                 470                 475                 480 


Val Phe Asn Lys Lys Thr Thr Thr Lys Thr Val Thr Lys Lys Pro Thr 
                485                 490                 495     


Pro Val Lys Thr Ala Pro Lys Leu Ala Glu Leu Pro Ala Thr Lys Pro 
            500                 505                 510         


Gln Glu Thr Val Leu Arg Glu Asn Lys Thr Pro Phe Ile Glu Lys Gln 
        515                 520                 525             


Ala Glu Thr Asn Lys Gln Ser Ile Asn Met Pro Ser Leu Pro Val Ile 
    530                 535                 540                 


Gln Lys Glu Ala Thr Glu Ser Asp Lys Glu Glu Met Lys Pro Gln Thr 
545                 550                 555                 560 


Glu Glu Lys Met Val Glu Glu Ser Glu Ser Ala Asn Asn Ala Asn Gly 
                565                 570                 575     


Lys Asn Arg Ser Ala Gly Ile Glu Glu Gly Lys Leu Ile Ala Lys Ser 
            580                 585                 590         


Ala Glu Asp Glu Lys Ala Lys Glu Glu Pro Gly Asn His Thr Thr Leu 
        595                 600                 605             


Ile Leu Ala Met Leu Ala Ile Gly Val Phe Ser Leu Gly Ala Phe Ile 
    610                 615                 620                 


Lys Ile Ile Gln Leu Arg Lys Asn Asn 
625                 630             


<210>  8
<211>  639
<212>  PRT
<213>  Listeria monocytogenes

<400>  8

Met Gly Leu Asn Arg Phe Met Arg Ala Met Met Val Val Phe Ile Thr 
1               5                   10                  15      


Ala Asn Cys Ile Thr Ile Asn Pro Asp Ile Ile Phe Ala Ala Thr Asp 
            20                  25                  30          


Ser Glu Asp Ser Ser Leu Asn Thr Asp Glu Trp Glu Glu Glu Lys Thr 
        35                  40                  45              


Glu Glu Gln Pro Ser Glu Val Asn Thr Gly Pro Arg Tyr Glu Thr Ala 
    50                  55                  60                  


Arg Glu Val Ser Ser Arg Asp Ile Glu Glu Leu Glu Lys Ser Asn Lys 
65                  70                  75                  80  


Val Lys Asn Thr Asn Lys Ala Asp Leu Ile Ala Met Leu Lys Ala Lys 
                85                  90                  95      


Ala Glu Lys Gly Pro Asn Asn Asn Asn Asn Asn Gly Glu Gln Thr Gly 
            100                 105                 110         


Asn Val Ala Ile Asn Glu Glu Ala Ser Gly Val Asp Arg Pro Thr Leu 
        115                 120                 125             


Gln Val Glu Arg Arg His Pro Gly Leu Ser Ser Asp Ser Ala Ala Glu 
    130                 135                 140                 


Ile Lys Lys Arg Arg Lys Ala Ile Ala Ser Ser Asp Ser Glu Leu Glu 
145                 150                 155                 160 


Ser Leu Thr Tyr Pro Asp Lys Pro Thr Lys Ala Asn Lys Arg Lys Val 
                165                 170                 175     


Ala Lys Glu Ser Val Val Asp Ala Ser Glu Ser Asp Leu Asp Ser Ser 
            180                 185                 190         


Met Gln Ser Ala Asp Glu Ser Thr Pro Gln Pro Leu Lys Ala Asn Gln 
        195                 200                 205             


Lys Pro Phe Phe Pro Lys Val Phe Lys Lys Ile Lys Asp Ala Gly Lys 
    210                 215                 220                 


Trp Val Arg Asp Lys Ile Asp Glu Asn Pro Glu Val Lys Lys Ala Ile 
225                 230                 235                 240 


Val Asp Lys Ser Ala Gly Leu Ile Asp Gln Leu Leu Thr Lys Lys Lys 
                245                 250                 255     


Ser Glu Glu Val Asn Ala Ser Asp Phe Pro Pro Pro Pro Thr Asp Glu 
            260                 265                 270         


Glu Leu Arg Leu Ala Leu Pro Glu Thr Pro Met Leu Leu Gly Phe Asn 
        275                 280                 285             


Ala Pro Thr Pro Ser Glu Pro Ser Ser Phe Glu Phe Pro Pro Pro Pro 
    290                 295                 300                 


Thr Asp Glu Glu Leu Arg Leu Ala Leu Pro Glu Thr Pro Met Leu Leu 
305                 310                 315                 320 


Gly Phe Asn Ala Pro Ala Thr Ser Glu Pro Ser Ser Phe Glu Phe Pro 
                325                 330                 335     


Pro Pro Pro Thr Glu Asp Glu Leu Glu Ile Met Arg Glu Thr Ala Pro 
            340                 345                 350         


Ser Leu Asp Ser Ser Phe Thr Ser Gly Asp Leu Ala Ser Leu Arg Ser 
        355                 360                 365             


Ala Ile Asn Arg His Ser Glu Asn Phe Ser Asp Phe Pro Leu Ile Pro 
    370                 375                 380                 


Thr Glu Glu Glu Leu Asn Gly Arg Gly Gly Arg Pro Thr Ser Glu Glu 
385                 390                 395                 400 


Phe Ser Ser Leu Asn Ser Gly Asp Phe Thr Asp Asp Glu Asn Ser Glu 
                405                 410                 415     


Thr Thr Glu Glu Glu Ile Asp Arg Leu Ala Asp Leu Arg Asp Arg Gly 
            420                 425                 430         


Thr Gly Lys His Ser Arg Asn Ala Gly Phe Leu Pro Leu Asn Pro Phe 
        435                 440                 445             


Ile Ser Ser Pro Val Pro Ser Leu Thr Pro Lys Val Pro Lys Ile Ser 
    450                 455                 460                 


Ala Pro Ala Leu Ile Ser Asp Ile Thr Lys Lys Ala Pro Phe Lys Asn 
465                 470                 475                 480 


Pro Ser Gln Pro Leu Asn Val Phe Asn Lys Lys Thr Thr Thr Lys Thr 
                485                 490                 495     


Val Thr Lys Lys Pro Thr Pro Val Lys Thr Ala Pro Lys Leu Ala Glu 
            500                 505                 510         


Leu Pro Ala Thr Lys Pro Gln Glu Thr Val Leu Arg Glu Asn Lys Thr 
        515                 520                 525             


Pro Phe Ile Glu Lys Gln Ala Glu Thr Asn Lys Gln Ser Ile Asn Met 
    530                 535                 540                 


Pro Ser Leu Pro Val Ile Gln Lys Glu Ala Thr Glu Ser Asp Lys Glu 
545                 550                 555                 560 


Glu Met Lys Pro Gln Thr Glu Glu Lys Met Val Glu Glu Ser Glu Ser 
                565                 570                 575     


Ala Asn Asn Ala Asn Gly Lys Asn Arg Ser Ala Gly Ile Glu Glu Gly 
            580                 585                 590         


Lys Leu Ile Ala Lys Ser Ala Glu Asp Glu Lys Ala Lys Glu Glu Pro 
        595                 600                 605             


Gly Asn His Thr Thr Leu Ile Leu Ala Met Leu Ala Ile Gly Val Phe 
    610                 615                 620                 


Ser Leu Gly Ala Phe Ile Lys Ile Ile Gln Leu Arg Lys Asn Asn 
625                 630                 635                 


<210>  9
<211>  390
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Truncated ActA

<400>  9

Met Arg Ala Met Met Val Val Phe Ile Thr Ala Asn Cys Ile Thr Ile 
1               5                   10                  15      


Asn Pro Asp Ile Ile Phe Ala Ala Thr Asp Ser Glu Asp Ser Ser Leu 
            20                  25                  30          


Asn Thr Asp Glu Trp Glu Glu Glu Lys Thr Glu Glu Gln Pro Ser Glu 
        35                  40                  45              


Val Asn Thr Gly Pro Arg Tyr Glu Thr Ala Arg Glu Val Ser Ser Arg 
    50                  55                  60                  


Asp Ile Lys Glu Leu Glu Lys Ser Asn Lys Val Arg Asn Thr Asn Lys 
65                  70                  75                  80  


Ala Asp Leu Ile Ala Met Leu Lys Glu Lys Ala Glu Lys Gly Pro Asn 
                85                  90                  95      


Ile Asn Asn Asn Asn Ser Glu Gln Thr Glu Asn Ala Ala Ile Asn Glu 
            100                 105                 110         


Glu Ala Ser Gly Ala Asp Arg Pro Ala Ile Gln Val Glu Arg Arg His 
        115                 120                 125             


Pro Gly Leu Pro Ser Asp Ser Ala Ala Glu Ile Lys Lys Arg Arg Lys 
    130                 135                 140                 


Ala Ile Ala Ser Ser Asp Ser Glu Leu Glu Ser Leu Thr Tyr Pro Asp 
145                 150                 155                 160 


Lys Pro Thr Lys Val Asn Lys Lys Lys Val Ala Lys Glu Ser Val Ala 
                165                 170                 175     


Asp Ala Ser Glu Ser Asp Leu Asp Ser Ser Met Gln Ser Ala Asp Glu 
            180                 185                 190         


Ser Ser Pro Gln Pro Leu Lys Ala Asn Gln Gln Pro Phe Phe Pro Lys 
        195                 200                 205             


Val Phe Lys Lys Ile Lys Asp Ala Gly Lys Trp Val Arg Asp Lys Ile 
    210                 215                 220                 


Asp Glu Asn Pro Glu Val Lys Lys Ala Ile Val Asp Lys Ser Ala Gly 
225                 230                 235                 240 


Leu Ile Asp Gln Leu Leu Thr Lys Lys Lys Ser Glu Glu Val Asn Ala 
                245                 250                 255     


Ser Asp Phe Pro Pro Pro Pro Thr Asp Glu Glu Leu Arg Leu Ala Leu 
            260                 265                 270         


Pro Glu Thr Pro Met Leu Leu Gly Phe Asn Ala Pro Ala Thr Ser Glu 
        275                 280                 285             


Pro Ser Ser Phe Glu Phe Pro Pro Pro Pro Thr Asp Glu Glu Leu Arg 
    290                 295                 300                 


Leu Ala Leu Pro Glu Thr Pro Met Leu Leu Gly Phe Asn Ala Pro Ala 
305                 310                 315                 320 


Thr Ser Glu Pro Ser Ser Phe Glu Phe Pro Pro Pro Pro Thr Glu Asp 
                325                 330                 335     


Glu Leu Glu Ile Ile Arg Glu Thr Ala Ser Ser Leu Asp Ser Ser Phe 
            340                 345                 350         


Thr Arg Gly Asp Leu Ala Ser Leu Arg Asn Ala Ile Asn Arg His Ser 
        355                 360                 365             


Gln Asn Phe Ser Asp Phe Pro Pro Ile Pro Thr Glu Glu Glu Leu Asn 
    370                 375                 380                 


Gly Arg Gly Gly Arg Pro 
385                 390 


<210>  10
<211>  100
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Truncated ActA

<400>  10

Met Gly Leu Asn Arg Phe Met Arg Ala Met Met Val Val Phe Ile Thr 
1               5                   10                  15      


Ala Asn Cys Ile Thr Ile Asn Pro Asp Ile Ile Phe Ala Ala Thr Asp 
            20                  25                  30          


Ser Glu Asp Ser Ser Leu Asn Thr Asp Glu Trp Glu Glu Glu Lys Thr 
        35                  40                  45              


Glu Glu Gln Pro Ser Glu Val Asn Thr Gly Pro Arg Tyr Glu Thr Ala 
    50                  55                  60                  


Arg Glu Val Ser Ser Arg Asp Ile Lys Glu Leu Glu Lys Ser Asn Lys 
65                  70                  75                  80  


Val Arg Asn Thr Asn Lys Ala Asp Leu Ile Ala Met Leu Lys Glu Lys 
                85                  90                  95      


Ala Glu Lys Gly 
            100 


<210>  11
<211>  93
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Truncated ActA

<400>  11

Ala Thr Asp Ser Glu Asp Ser Ser Leu Asn Thr Asp Glu Trp Glu Glu 
1               5                   10                  15      


Glu Lys Thr Glu Glu Gln Pro Ser Glu Val Asn Thr Gly Pro Arg Tyr 
            20                  25                  30          


Glu Thr Ala Arg Glu Val Ser Ser Arg Asp Ile Glu Glu Leu Glu Lys 
        35                  40                  45              


Ser Asn Lys Val Lys Asn Thr Asn Lys Ala Asp Leu Ile Ala Met Leu 
    50                  55                  60                  


Lys Ala Lys Ala Glu Lys Gly Pro Asn Asn Asn Asn Asn Asn Gly Glu 
65                  70                  75                  80  


Gln Thr Gly Asn Val Ala Ile Asn Glu Glu Ala Ser Gly 
                85                  90              


<210>  12
<211>  200
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Truncated ActA

<400>  12

Ala Thr Asp Ser Glu Asp Ser Ser Leu Asn Thr Asp Glu Trp Glu Glu 
1               5                   10                  15      


Glu Lys Thr Glu Glu Gln Pro Ser Glu Val Asn Thr Gly Pro Arg Tyr 
            20                  25                  30          


Glu Thr Ala Arg Glu Val Ser Ser Arg Asp Ile Glu Glu Leu Glu Lys 
        35                  40                  45              


Ser Asn Lys Val Lys Asn Thr Asn Lys Ala Asp Leu Ile Ala Met Leu 
    50                  55                  60                  


Lys Ala Lys Ala Glu Lys Gly Pro Asn Asn Asn Asn Asn Asn Gly Glu 
65                  70                  75                  80  


Gln Thr Gly Asn Val Ala Ile Asn Glu Glu Ala Ser Gly Val Asp Arg 
                85                  90                  95      


Pro Thr Leu Gln Val Glu Arg Arg His Pro Gly Leu Ser Ser Asp Ser 
            100                 105                 110         


Ala Ala Glu Ile Lys Lys Arg Arg Lys Ala Ile Ala Ser Ser Asp Ser 
        115                 120                 125             


Glu Leu Glu Ser Leu Thr Tyr Pro Asp Lys Pro Thr Lys Ala Asn Lys 
    130                 135                 140                 


Arg Lys Val Ala Lys Glu Ser Val Val Asp Ala Ser Glu Ser Asp Leu 
145                 150                 155                 160 


Asp Ser Ser Met Gln Ser Ala Asp Glu Ser Thr Pro Gln Pro Leu Lys 
                165                 170                 175     


Ala Asn Gln Lys Pro Phe Phe Pro Lys Val Phe Lys Lys Ile Lys Asp 
            180                 185                 190         


Ala Gly Lys Trp Val Arg Asp Lys 
        195                 200 


<210>  13
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Truncated ActA

<400>  13

Ala Thr Asp Ser Glu Asp Ser Ser Leu Asn Thr Asp Glu Trp Glu Glu 
1               5                   10                  15      


Glu Lys Thr Glu Glu Gln Pro Ser Glu Val Asn Thr Gly Pro Arg Tyr 
            20                  25                  30          


Glu Thr Ala Arg Glu Val Ser Ser Arg Asp Ile Glu Glu Leu Glu Lys 
        35                  40                  45              


Ser Asn Lys Val Lys Asn Thr Asn Lys Ala Asp Leu Ile Ala Met Leu 
    50                  55                  60                  


Lys Ala Lys Ala Glu Lys Gly Pro Asn Asn Asn Asn Asn Asn Gly Glu 
65                  70                  75                  80  


Gln Thr Gly Asn Val Ala Ile Asn Glu Glu Ala Ser Gly Val Asp Arg 
                85                  90                  95      


Pro Thr Leu Gln Val Glu Arg Arg His Pro Gly Leu Ser Ser Asp Ser 
            100                 105                 110         


Ala Ala Glu Ile Lys Lys Arg Arg Lys Ala Ile Ala Ser Ser Asp Ser 
        115                 120                 125             


Glu Leu Glu Ser Leu Thr Tyr Pro Asp Lys Pro Thr Lys Ala Asn Lys 
    130                 135                 140                 


Arg Lys Val Ala Lys Glu Ser Val Val Asp Ala Ser Glu Ser Asp Leu 
145                 150                 155                 160 


Asp Ser Ser Met Gln Ser Ala Asp Glu Ser Thr Pro Gln Pro Leu Lys 
                165                 170                 175     


Ala Asn Gln Lys Pro Phe Phe Pro Lys Val Phe Lys Lys Ile Lys Asp 
            180                 185                 190         


Ala Gly Lys Trp Val Arg Asp Lys Ile Asp Glu Asn Pro Glu Val Lys 
        195                 200                 205             


Lys Ala Ile Val Asp Lys Ser Ala Gly Leu Ile Asp Gln Leu Leu Thr 
    210                 215                 220                 


Lys Lys Lys Ser Glu Glu Val Asn Ala Ser Asp Phe Pro Pro Pro Pro 
225                 230                 235                 240 


Thr Asp Glu Glu Leu Arg Leu Ala Leu Pro Glu Thr Pro Met Leu Leu 
                245                 250                 255     


Gly Phe Asn Ala Pro Thr Pro Ser Glu Pro Ser Ser Phe Glu Phe Pro 
            260                 265                 270         


Pro Pro Pro Thr Asp Glu Glu Leu Arg Leu Ala Leu Pro Glu Thr Pro 
        275                 280                 285             


Met Leu Leu Gly Phe Asn Ala Pro Ala Thr Ser Glu Pro Ser Ser 
    290                 295                 300             


<210>  14
<211>  370
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Truncated ActA

<400>  14

Ala Thr Asp Ser Glu Asp Ser Ser Leu Asn Thr Asp Glu Trp Glu Glu 
1               5                   10                  15      


Glu Lys Thr Glu Glu Gln Pro Ser Glu Val Asn Thr Gly Pro Arg Tyr 
            20                  25                  30          


Glu Thr Ala Arg Glu Val Ser Ser Arg Asp Ile Glu Glu Leu Glu Lys 
        35                  40                  45              


Ser Asn Lys Val Lys Asn Thr Asn Lys Ala Asp Leu Ile Ala Met Leu 
    50                  55                  60                  


Lys Ala Lys Ala Glu Lys Gly Pro Asn Asn Asn Asn Asn Asn Gly Glu 
65                  70                  75                  80  


Gln Thr Gly Asn Val Ala Ile Asn Glu Glu Ala Ser Gly Val Asp Arg 
                85                  90                  95      


Pro Thr Leu Gln Val Glu Arg Arg His Pro Gly Leu Ser Ser Asp Ser 
            100                 105                 110         


Ala Ala Glu Ile Lys Lys Arg Arg Lys Ala Ile Ala Ser Ser Asp Ser 
        115                 120                 125             


Glu Leu Glu Ser Leu Thr Tyr Pro Asp Lys Pro Thr Lys Ala Asn Lys 
    130                 135                 140                 


Arg Lys Val Ala Lys Glu Ser Val Val Asp Ala Ser Glu Ser Asp Leu 
145                 150                 155                 160 


Asp Ser Ser Met Gln Ser Ala Asp Glu Ser Thr Pro Gln Pro Leu Lys 
                165                 170                 175     


Ala Asn Gln Lys Pro Phe Phe Pro Lys Val Phe Lys Lys Ile Lys Asp 
            180                 185                 190         


Ala Gly Lys Trp Val Arg Asp Lys Ile Asp Glu Asn Pro Glu Val Lys 
        195                 200                 205             


Lys Ala Ile Val Asp Lys Ser Ala Gly Leu Ile Asp Gln Leu Leu Thr 
    210                 215                 220                 


Lys Lys Lys Ser Glu Glu Val Asn Ala Ser Asp Phe Pro Pro Pro Pro 
225                 230                 235                 240 


Thr Asp Glu Glu Leu Arg Leu Ala Leu Pro Glu Thr Pro Met Leu Leu 
                245                 250                 255     


Gly Phe Asn Ala Pro Thr Pro Ser Glu Pro Ser Ser Phe Glu Phe Pro 
            260                 265                 270         


Pro Pro Pro Thr Asp Glu Glu Leu Arg Leu Ala Leu Pro Glu Thr Pro 
        275                 280                 285             


Met Leu Leu Gly Phe Asn Ala Pro Ala Thr Ser Glu Pro Ser Ser Phe 
    290                 295                 300                 


Glu Phe Pro Pro Pro Pro Thr Glu Asp Glu Leu Glu Ile Met Arg Glu 
305                 310                 315                 320 


Thr Ala Pro Ser Leu Asp Ser Ser Phe Thr Ser Gly Asp Leu Ala Ser 
                325                 330                 335     


Leu Arg Ser Ala Ile Asn Arg His Ser Glu Asn Phe Ser Asp Phe Pro 
            340                 345                 350         


Leu Ile Pro Thr Glu Glu Glu Leu Asn Gly Arg Gly Gly Arg Pro Thr 
        355                 360                 365             


Ser Glu 
    370 


<210>  15
<211>  1170
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Truncated ActA

<400>  15
atgcgtgcga tgatggtggt tttcattact gccaattgca ttacgattaa ccccgacata       60

atatttgcag cgacagatag cgaagattct agtctaaaca cagatgaatg ggaagaagaa      120

aaaacagaag agcaaccaag cgaggtaaat acgggaccaa gatacgaaac tgcacgtgaa      180

gtaagttcac gtgatattaa agaactagaa aaatcgaata aagtgagaaa tacgaacaaa      240

gcagacctaa tagcaatgtt gaaagaaaaa gcagaaaaag gtccaaatat caataataac      300

aacagtgaac aaactgagaa tgcggctata aatgaagagg cttcaggagc cgaccgacca      360

gctatacaag tggagcgtcg tcatccagga ttgccatcgg atagcgcagc ggaaattaaa      420

aaaagaagga aagccatagc atcatcggat agtgagcttg aaagccttac ttatccggat      480

aaaccaacaa aagtaaataa gaaaaaagtg gcgaaagagt cagttgcgga tgcttctgaa      540

agtgacttag attctagcat gcagtcagca gatgagtctt caccacaacc tttaaaagca      600

aaccaacaac catttttccc taaagtattt aaaaaaataa aagatgcggg gaaatgggta      660

cgtgataaaa tcgacgaaaa tcctgaagta aagaaagcga ttgttgataa aagtgcaggg      720

ttaattgacc aattattaac caaaaagaaa agtgaagagg taaatgcttc ggacttcccg      780

ccaccaccta cggatgaaga gttaagactt gctttgccag agacaccaat gcttcttggt      840

tttaatgctc ctgctacatc agaaccgagc tcattcgaat ttccaccacc acctacggat      900

gaagagttaa gacttgcttt gccagagacg ccaatgcttc ttggttttaa tgctcctgct      960

acatcggaac cgagctcgtt cgaatttcca ccgcctccaa cagaagatga actagaaatc     1020

atccgggaaa cagcatcctc gctagattct agttttacaa gaggggattt agctagtttg     1080

agaaatgcta ttaatcgcca tagtcaaaat ttctctgatt tcccaccaat cccaacagaa     1140

gaagagttga acgggagagg cggtagacca                                      1170


