                         SEQUENCE LISTING

<110>  DNA Polymerase, Inc.
 
<120>  Mutant Polymerases and Uses Thereof

<130>  60019630-000107

<150>  62/160,404
<151>  2015-05-12

<160>  32    

<170>  PatentIn version 3.5

<210>  1
<211>  832
<212>  PRT
<213>  Thermus aquaticus

<400>  1

Met Arg Gly Met Leu Pro Leu Phe Glu Pro Lys Gly Arg Val Leu Leu 
1               5                   10                  15      


Val Asp Gly His His Leu Ala Tyr Arg Thr Phe His Ala Leu Lys Gly 
            20                  25                  30          


Leu Thr Thr Ser Arg Gly Glu Pro Val Gln Ala Val Tyr Gly Phe Ala 
        35                  40                  45              


Lys Ser Leu Leu Lys Ala Leu Lys Glu Asp Gly Asp Ala Val Ile Val 
    50                  55                  60                  


Val Phe Asp Ala Lys Ala Pro Ser Phe Arg His Glu Ala Tyr Gly Gly 
65                  70                  75                  80  


Tyr Lys Ala Gly Arg Ala Pro Thr Pro Glu Asp Phe Pro Arg Gln Leu 
                85                  90                  95      


Ala Leu Ile Lys Glu Leu Val Asp Leu Leu Gly Leu Ala Arg Leu Glu 
            100                 105                 110         


Val Pro Gly Tyr Glu Ala Asp Asp Val Leu Ala Ser Leu Ala Lys Lys 
        115                 120                 125             


Ala Glu Lys Glu Gly Tyr Glu Val Arg Ile Leu Thr Ala Asp Lys Asp 
    130                 135                 140                 


Leu Tyr Gln Leu Leu Ser Asp Arg Ile His Val Leu His Pro Glu Gly 
145                 150                 155                 160 


Tyr Leu Ile Thr Pro Ala Trp Leu Trp Glu Lys Tyr Gly Leu Arg Pro 
                165                 170                 175     


Asp Gln Trp Ala Asp Tyr Arg Ala Leu Thr Gly Asp Glu Ser Asp Asn 
            180                 185                 190         


Leu Pro Gly Val Lys Gly Ile Gly Glu Lys Thr Ala Arg Lys Leu Leu 
        195                 200                 205             


Glu Glu Trp Gly Ser Leu Glu Ala Leu Leu Lys Asn Leu Asp Arg Leu 
    210                 215                 220                 


Lys Pro Ala Ile Arg Glu Lys Ile Leu Ala His Met Asp Asp Leu Lys 
225                 230                 235                 240 


Leu Ser Trp Asp Leu Ala Lys Val Arg Thr Asp Leu Pro Leu Glu Val 
                245                 250                 255     


Asp Phe Ala Lys Arg Arg Glu Pro Asp Arg Glu Arg Leu Arg Ala Phe 
            260                 265                 270         


Leu Glu Arg Leu Glu Phe Gly Ser Leu Leu His Glu Phe Gly Leu Leu 
        275                 280                 285             


Glu Ser Pro Lys Ala Leu Glu Glu Ala Pro Trp Pro Pro Pro Glu Gly 
    290                 295                 300                 


Ala Phe Val Gly Phe Val Leu Ser Arg Lys Glu Pro Met Trp Ala Asp 
305                 310                 315                 320 


Leu Leu Ala Leu Ala Ala Ala Arg Gly Gly Arg Val His Arg Ala Pro 
                325                 330                 335     


Glu Pro Tyr Lys Ala Leu Arg Asp Leu Lys Glu Ala Arg Gly Leu Leu 
            340                 345                 350         


Ala Lys Asp Leu Ser Val Leu Ala Leu Arg Glu Gly Leu Gly Leu Pro 
        355                 360                 365             


Pro Gly Asp Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro Ser Asn 
    370                 375                 380                 


Thr Thr Pro Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp Thr Glu 
385                 390                 395                 400 


Glu Ala Gly Glu Arg Ala Ala Leu Ser Glu Arg Leu Phe Ala Asn Leu 
                405                 410                 415     


Trp Gly Arg Leu Glu Gly Glu Glu Arg Leu Leu Trp Leu Tyr Arg Glu 
            420                 425                 430         


Val Glu Arg Pro Leu Ser Ala Val Leu Ala His Met Glu Ala Thr Gly 
        435                 440                 445             


Val Arg Leu Asp Val Ala Tyr Leu Arg Ala Leu Ser Leu Glu Val Ala 
    450                 455                 460                 


Glu Glu Ile Ala Arg Leu Glu Ala Glu Val Phe Arg Leu Ala Gly His 
465                 470                 475                 480 


Pro Phe Asn Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu Phe Asp 
                485                 490                 495     


Glu Leu Gly Leu Pro Ala Ile Gly Lys Thr Glu Lys Thr Gly Lys Arg 
            500                 505                 510         


Ser Thr Ser Ala Ala Val Leu Glu Ala Leu Arg Glu Ala His Pro Ile 
        515                 520                 525             


Val Glu Lys Ile Leu Gln Tyr Arg Glu Leu Thr Lys Leu Lys Ser Thr 
    530                 535                 540                 


Tyr Ile Asp Pro Leu Pro Asp Leu Ile His Pro Arg Thr Gly Arg Leu 
545                 550                 555                 560 


His Thr Arg Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu Ser Ser 
                565                 570                 575     


Ser Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Thr Pro Leu Gly Gln 
            580                 585                 590         


Arg Ile Arg Arg Ala Phe Ile Ala Glu Glu Gly Trp Leu Leu Val Ala 
        595                 600                 605             


Leu Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu Ser Gly 
    610                 615                 620                 


Asp Glu Asn Leu Ile Arg Val Phe Gln Glu Gly Arg Asp Ile His Thr 
625                 630                 635                 640 


Glu Thr Ala Ser Trp Met Phe Gly Val Pro Arg Glu Ala Val Asp Pro 
                645                 650                 655     


Leu Met Arg Arg Ala Ala Lys Thr Ile Asn Phe Gly Val Leu Tyr Gly 
            660                 665                 670         


Met Ser Ala His Arg Leu Ser Gln Glu Leu Ala Ile Pro Tyr Glu Glu 
        675                 680                 685             


Ala Gln Ala Phe Ile Glu Arg Tyr Phe Gln Ser Phe Pro Lys Val Arg 
    690                 695                 700                 


Ala Trp Ile Glu Lys Thr Leu Glu Glu Gly Arg Arg Arg Gly Tyr Val 
705                 710                 715                 720 


Glu Thr Leu Phe Gly Arg Arg Arg Tyr Val Pro Asp Leu Glu Ala Arg 
                725                 730                 735     


Val Lys Ser Val Arg Glu Ala Ala Glu Arg Met Ala Phe Asn Met Pro 
            740                 745                 750         


Val Gln Gly Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val Lys Leu 
        755                 760                 765             


Phe Pro Arg Leu Glu Glu Met Gly Ala Arg Met Leu Leu Gln Val His 
    770                 775                 780                 


Asp Glu Leu Val Leu Glu Ala Pro Lys Glu Arg Ala Glu Ala Val Ala 
785                 790                 795                 800 


Arg Leu Ala Lys Glu Val Met Glu Gly Val Tyr Pro Leu Ala Val Pro 
                805                 810                 815     


Leu Glu Val Glu Val Gly Ile Gly Glu Asp Trp Leu Ser Ala Lys Glu 
            820                 825                 830         


<210>  2
<211>  554
<212>  PRT
<213>  Thermus aquaticus

<400>  2

Met Gly Leu Leu His Glu Phe Gly Leu Leu Glu Ser Pro Lys Ala Leu 
1               5                   10                  15      


Glu Glu Ala Pro Trp Pro Pro Pro Glu Gly Ala Phe Val Gly Phe Val 
            20                  25                  30          


Leu Ser Arg Lys Glu Pro Met Trp Ala Asp Leu Leu Ala Leu Ala Ala 
        35                  40                  45              


Ala Arg Gly Gly Arg Val His Arg Ala Pro Glu Pro Tyr Lys Ala Leu 
    50                  55                  60                  


Arg Asp Leu Lys Glu Ala Arg Gly Leu Leu Ala Lys Asp Leu Ser Val 
65                  70                  75                  80  


Leu Ala Leu Arg Glu Gly Leu Gly Leu Pro Pro Gly Asp Asp Pro Met 
                85                  90                  95      


Leu Leu Ala Tyr Leu Leu Asp Pro Ser Asn Thr Thr Pro Glu Gly Val 
            100                 105                 110         


Ala Arg Arg Tyr Gly Gly Glu Trp Thr Glu Glu Ala Gly Glu Arg Ala 
        115                 120                 125             


Ala Leu Ser Glu Arg Leu Phe Ala Asn Leu Trp Gly Arg Leu Glu Gly 
    130                 135                 140                 


Glu Glu Arg Leu Leu Trp Leu Tyr Arg Glu Val Glu Arg Pro Leu Ser 
145                 150                 155                 160 


Ala Val Leu Ala His Met Glu Ala Thr Gly Val Arg Leu Asp Val Ala 
                165                 170                 175     


Tyr Leu Arg Ala Leu Ser Leu Glu Val Ala Glu Glu Ile Ala Arg Leu 
            180                 185                 190         


Glu Ala Glu Val Phe Arg Leu Ala Gly His Pro Phe Asn Leu Asn Ser 
        195                 200                 205             


Arg Asp Gln Leu Glu Arg Val Leu Phe Asp Glu Leu Gly Leu Pro Ala 
    210                 215                 220                 


Ile Gly Lys Thr Glu Lys Thr Gly Lys Arg Ser Thr Ser Ala Ala Val 
225                 230                 235                 240 


Leu Glu Ala Leu Arg Glu Ala His Pro Ile Val Glu Lys Ile Leu Gln 
                245                 250                 255     


Tyr Arg Glu Leu Thr Lys Leu Lys Ser Thr Tyr Ile Asp Pro Leu Pro 
            260                 265                 270         


Asp Leu Ile His Pro Arg Thr Gly Arg Leu His Thr Arg Phe Asn Gln 
        275                 280                 285             


Thr Ala Thr Ala Thr Gly Arg Leu Ser Ser Ser Asp Pro Asn Leu Gln 
    290                 295                 300                 


Asn Ile Pro Val Arg Thr Pro Leu Gly Gln Arg Ile Arg Arg Ala Phe 
305                 310                 315                 320 


Ile Ala Glu Glu Gly Trp Leu Leu Val Ala Leu Asp Tyr Ser Gln Ile 
                325                 330                 335     


Glu Leu Arg Val Leu Ala His Leu Ser Gly Asp Lys Asn Leu Ile Arg 
            340                 345                 350         


Val Phe Gln Glu Gly Arg Asp Ile His Thr Glu Thr Ala Ser Trp Met 
        355                 360                 365             


Phe Gly Val Pro Arg Glu Ala Val Asp Pro Leu Met Arg Arg Ala Ala 
    370                 375                 380                 


Lys Thr Ile Asn Phe Gly Val Leu Tyr Gly Met Ser Ala His Arg Leu 
385                 390                 395                 400 


Ser Gln Glu Leu Ala Ile Pro Tyr Glu Glu Ala Gln Ala Phe Ile Glu 
                405                 410                 415     


Arg Tyr Phe Gln Ser Phe Pro Lys Val Arg Ala Trp Leu Lys Lys Thr 
            420                 425                 430         


Leu Glu Glu Gly Arg Arg Arg Gly Tyr Val Glu Thr Leu Phe Gly Arg 
        435                 440                 445             


Arg Arg Tyr Val Pro Asp Leu Glu Ala Arg Val Lys Ser Val Arg Glu 
    450                 455                 460                 


Ala Ala Glu Arg Met Ala Phe Asn Met Pro Val Gln Gly Thr Ala Ala 
465                 470                 475                 480 


Asp Leu Met Lys Leu Ala Met Val Lys Leu Phe Pro Arg Leu Glu Glu 
                485                 490                 495     


Met Gly Ala Arg Met Leu Leu Gln Val His Asp Glu Leu Val Leu Glu 
            500                 505                 510         


Ala Pro Lys Glu Arg Ala Glu Ala Val Ala Arg Leu Ala Lys Glu Val 
        515                 520                 525             


Met Glu Gly Val Tyr Pro Leu Ala Val Pro Leu Glu Val Glu Val Gly 
    530                 535                 540                 


Ile Gly Glu Asp Trp Leu Ser Ala Lys Glu 
545                 550                 


<210>  3
<211>  832
<212>  PRT
<213>  Thermus aquaticus

<400>  3

Met Arg Gly Met Leu Pro Leu Phe Glu Pro Lys Gly Arg Val Leu Leu 
1               5                   10                  15      


Val Asp Gly His His Leu Ala Tyr Arg Thr Phe His Ala Leu Lys Gly 
            20                  25                  30          


Leu Thr Thr Ser Arg Gly Glu Pro Val Gln Ala Val Tyr Gly Phe Ala 
        35                  40                  45              


Lys Ser Leu Leu Lys Ala Leu Lys Glu Asp Gly Asp Ala Val Ile Val 
    50                  55                  60                  


Val Phe Asp Ala Lys Ala Pro Ser Phe Arg His Glu Ala Tyr Gly Gly 
65                  70                  75                  80  


Tyr Lys Ala Gly Arg Ala Pro Thr Pro Glu Asp Phe Pro Arg Gln Leu 
                85                  90                  95      


Ala Leu Ile Lys Glu Leu Val Asp Leu Leu Gly Leu Ala Arg Leu Glu 
            100                 105                 110         


Val Pro Gly Tyr Glu Ala Asp Asp Val Leu Ala Ser Leu Ala Lys Lys 
        115                 120                 125             


Ala Glu Lys Glu Gly Tyr Glu Val Arg Ile Leu Thr Ala Asp Lys Asp 
    130                 135                 140                 


Leu Tyr Gln Leu Leu Ser Asp Arg Ile His Val Leu His Pro Glu Gly 
145                 150                 155                 160 


Tyr Leu Ile Thr Pro Ala Trp Leu Trp Glu Lys Tyr Gly Leu Arg Pro 
                165                 170                 175     


Asp Gln Trp Ala Asp Tyr Arg Ala Leu Thr Gly Asp Glu Ser Asp Asn 
            180                 185                 190         


Leu Pro Gly Val Lys Gly Ile Gly Glu Lys Thr Ala Arg Lys Leu Leu 
        195                 200                 205             


Glu Glu Trp Gly Ser Leu Glu Ala Leu Leu Lys Asn Leu Asp Arg Leu 
    210                 215                 220                 


Lys Pro Ala Ile Arg Glu Lys Ile Leu Ala His Met Asp Asp Leu Lys 
225                 230                 235                 240 


Leu Ser Trp Asp Leu Ala Lys Val Arg Thr Asp Leu Pro Leu Glu Val 
                245                 250                 255     


Asp Phe Ala Lys Arg Arg Glu Pro Asp Arg Glu Arg Leu Arg Ala Phe 
            260                 265                 270         


Leu Glu Arg Leu Glu Phe Gly Ser Leu Leu His Glu Phe Gly Leu Leu 
        275                 280                 285             


Glu Ser Pro Lys Ala Leu Glu Glu Ala Pro Trp Pro Pro Pro Glu Gly 
    290                 295                 300                 


Ala Phe Val Gly Phe Val Leu Ser Arg Lys Glu Pro Met Trp Ala Asp 
305                 310                 315                 320 


Leu Leu Ala Leu Ala Ala Ala Arg Gly Gly Arg Val His Arg Ala Pro 
                325                 330                 335     


Glu Pro Tyr Lys Ala Leu Arg Asp Leu Lys Glu Ala Arg Gly Leu Leu 
            340                 345                 350         


Ala Lys Asp Leu Ser Val Leu Ala Leu Arg Glu Gly Leu Gly Leu Pro 
        355                 360                 365             


Pro Gly Asp Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro Ser Asn 
    370                 375                 380                 


Thr Thr Pro Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp Thr Glu 
385                 390                 395                 400 


Glu Ala Gly Glu Arg Ala Ala Leu Ser Glu Arg Leu Phe Ala Asn Leu 
                405                 410                 415     


Trp Gly Arg Leu Glu Gly Glu Glu Arg Leu Leu Trp Leu Tyr Arg Glu 
            420                 425                 430         


Val Glu Arg Pro Leu Ser Ala Val Leu Ala His Met Glu Ala Thr Gly 
        435                 440                 445             


Val Arg Leu Asp Val Ala Tyr Leu Arg Ala Leu Ser Leu Glu Val Ala 
    450                 455                 460                 


Glu Glu Ile Ala Arg Leu Glu Ala Glu Val Phe Arg Leu Ala Gly His 
465                 470                 475                 480 


Pro Phe Asn Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu Phe Asp 
                485                 490                 495     


Glu Leu Gly Leu Pro Ala Ile Gly Lys Thr Glu Lys Thr Gly Lys Arg 
            500                 505                 510         


Ser Thr Ser Ala Ala Val Leu Glu Ala Leu Arg Glu Ala His Pro Ile 
        515                 520                 525             


Val Glu Lys Ile Leu Gln Tyr Arg Glu Leu Thr Lys Leu Lys Ser Thr 
    530                 535                 540                 


Tyr Ile Asp Pro Leu Pro Asp Leu Ile His Pro Arg Thr Gly Arg Leu 
545                 550                 555                 560 


His Thr Arg Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu Ser Ser 
                565                 570                 575     


Ser Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Thr Pro Leu Gly Gln 
            580                 585                 590         


Arg Ile Arg Arg Ala Phe Ile Ala Glu Glu Gly Trp Leu Leu Val Ala 
        595                 600                 605             


Leu Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu Ser Gly 
    610                 615                 620                 


Asp Glu Asn Leu Ile Arg Val Phe Gln Glu Gly Arg Asp Ile His Thr 
625                 630                 635                 640 


Glu Thr Ala Ser Trp Met Phe Gly Val Pro Arg Glu Ala Val Asp Pro 
                645                 650                 655     


Leu Met Arg Arg Ala Ala Lys Thr Ile Asn Phe Gly Val Leu Tyr Gly 
            660                 665                 670         


Met Ser Ala His Arg Leu Ser Gln Glu Leu Ala Ile Pro Tyr Glu Glu 
        675                 680                 685             


Ala Gln Ala Phe Ile Glu Arg Tyr Phe Gln Ser Phe Pro Lys Val Arg 
    690                 695                 700                 


Ala Trp Ile Glu Lys Thr Leu Glu Glu Gly Arg Arg Arg Gly Tyr Val 
705                 710                 715                 720 


Glu Thr Leu Phe Gly Arg Arg Arg Tyr Val Pro Asn Leu Glu Ala Arg 
                725                 730                 735     


Val Lys Ser Val Arg Glu Ala Ala Glu Arg Met Ala Phe Asn Met Pro 
            740                 745                 750         


Val Gln Gly Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val Lys Leu 
        755                 760                 765             


Phe Pro Arg Leu Glu Glu Met Gly Ala Arg Met Leu Leu Gln Val His 
    770                 775                 780                 


Asp Glu Leu Val Leu Glu Ala Pro Lys Glu Arg Ala Glu Ala Val Ala 
785                 790                 795                 800 


Arg Leu Ala Lys Glu Val Met Glu Gly Val Tyr Pro Leu Ala Val Pro 
                805                 810                 815     


Leu Glu Val Glu Val Gly Ile Gly Glu Asp Trp Leu Ser Ala Lys Glu 
            820                 825                 830         


<210>  4
<211>  554
<212>  PRT
<213>  Thermus aquaticus

<400>  4

Met Gly Leu Leu His Glu Phe Gly Leu Leu Glu Ser Pro Lys Ala Leu 
1               5                   10                  15      


Glu Glu Ala Pro Trp Pro Pro Pro Glu Gly Ala Phe Val Gly Phe Val 
            20                  25                  30          


Leu Ser Arg Lys Glu Pro Met Trp Ala Asp Leu Leu Ala Leu Ala Ala 
        35                  40                  45              


Ala Arg Gly Gly Arg Val His Arg Ala Pro Glu Pro Tyr Lys Ala Leu 
    50                  55                  60                  


Arg Asp Leu Lys Glu Ala Arg Gly Leu Leu Ala Lys Asp Leu Ser Val 
65                  70                  75                  80  


Leu Ala Leu Arg Glu Gly Leu Gly Leu Pro Pro Gly Asp Asp Pro Met 
                85                  90                  95      


Leu Leu Ala Tyr Leu Leu Asp Pro Ser Asn Thr Thr Pro Glu Gly Val 
            100                 105                 110         


Ala Arg Arg Tyr Gly Gly Glu Trp Thr Glu Glu Ala Gly Glu Arg Ala 
        115                 120                 125             


Ala Leu Ser Glu Arg Leu Phe Ala Asn Leu Trp Gly Arg Leu Glu Gly 
    130                 135                 140                 


Glu Glu Arg Leu Leu Trp Leu Tyr Arg Glu Val Glu Arg Pro Leu Ser 
145                 150                 155                 160 


Ala Val Leu Ala His Met Glu Ala Thr Gly Val Arg Leu Asp Val Ala 
                165                 170                 175     


Tyr Leu Arg Ala Leu Ser Leu Glu Val Ala Glu Glu Ile Ala Arg Leu 
            180                 185                 190         


Glu Ala Glu Val Phe Arg Leu Ala Gly His Pro Phe Asn Leu Asn Ser 
        195                 200                 205             


Arg Asp Gln Leu Glu Arg Val Leu Phe Asp Glu Leu Gly Leu Pro Ala 
    210                 215                 220                 


Ile Gly Lys Thr Glu Lys Thr Gly Lys Arg Ser Thr Ser Ala Ala Val 
225                 230                 235                 240 


Leu Glu Ala Leu Arg Glu Ala His Pro Ile Val Glu Lys Ile Leu Gln 
                245                 250                 255     


Tyr Arg Glu Leu Thr Lys Leu Lys Ser Thr Tyr Ile Asp Pro Leu Pro 
            260                 265                 270         


Asp Leu Ile His Pro Arg Thr Gly Arg Leu His Thr Arg Phe Asn Gln 
        275                 280                 285             


Thr Ala Thr Ala Thr Gly Arg Leu Ser Ser Ser Asp Pro Asn Leu Gln 
    290                 295                 300                 


Asn Ile Pro Val Arg Thr Pro Leu Gly Gln Arg Ile Arg Arg Ala Phe 
305                 310                 315                 320 


Ile Ala Glu Glu Gly Trp Leu Leu Val Ala Leu Asp Tyr Ser Gln Ile 
                325                 330                 335     


Glu Leu Arg Val Leu Ala His Leu Ser Gly Asp Lys Asn Leu Ile Arg 
            340                 345                 350         


Val Phe Gln Glu Gly Arg Asp Ile His Thr Glu Thr Ala Ser Trp Met 
        355                 360                 365             


Phe Gly Val Pro Arg Glu Ala Val Asp Pro Leu Met Arg Arg Ala Ala 
    370                 375                 380                 


Lys Thr Ile Asn Phe Gly Val Leu Tyr Gly Met Ser Ala His Arg Leu 
385                 390                 395                 400 


Ser Gln Glu Leu Ala Ile Pro Tyr Glu Glu Ala Gln Ala Phe Ile Glu 
                405                 410                 415     


Arg Tyr Phe Gln Ser Phe Pro Lys Val Arg Ala Trp Leu Lys Lys Thr 
            420                 425                 430         


Leu Glu Glu Gly Arg Arg Arg Gly Tyr Val Glu Thr Leu Phe Gly Arg 
        435                 440                 445             


Arg Arg Tyr Val Pro Asn Leu Glu Ala Arg Val Lys Ser Val Arg Glu 
    450                 455                 460                 


Ala Ala Glu Arg Met Ala Phe Asn Met Pro Val Gln Gly Thr Ala Ala 
465                 470                 475                 480 


Asp Leu Met Lys Leu Ala Met Val Lys Leu Phe Pro Arg Leu Glu Glu 
                485                 490                 495     


Met Gly Ala Arg Met Leu Leu Gln Val His Asp Glu Leu Val Leu Glu 
            500                 505                 510         


Ala Pro Lys Glu Arg Ala Glu Ala Val Ala Arg Leu Ala Lys Glu Val 
        515                 520                 525             


Met Glu Gly Val Tyr Pro Leu Ala Val Pro Leu Glu Val Glu Val Gly 
    530                 535                 540                 


Ile Gly Glu Asp Trp Leu Ser Ala Lys Glu 
545                 550                 


<210>  5
<211>  832
<212>  PRT
<213>  Thermus aquaticus

<400>  5

Met Arg Gly Met Leu Pro Leu Phe Glu Pro Lys Gly Arg Val Leu Leu 
1               5                   10                  15      


Val Asp Gly His His Leu Ala Tyr Arg Thr Phe His Ala Leu Lys Gly 
            20                  25                  30          


Leu Thr Thr Ser Arg Gly Glu Pro Val Gln Ala Val Tyr Gly Phe Ala 
        35                  40                  45              


Lys Ser Leu Leu Lys Ala Leu Lys Glu Asp Gly Asp Ala Val Ile Val 
    50                  55                  60                  


Val Phe Asp Ala Lys Ala Pro Ser Phe Arg His Glu Ala Tyr Gly Gly 
65                  70                  75                  80  


Tyr Lys Ala Gly Arg Ala Pro Thr Pro Glu Asp Phe Pro Arg Gln Leu 
                85                  90                  95      


Ala Leu Ile Lys Glu Leu Val Asp Leu Leu Gly Leu Ala Arg Leu Glu 
            100                 105                 110         


Val Pro Gly Tyr Glu Ala Asp Asp Val Leu Ala Ser Leu Ala Lys Lys 
        115                 120                 125             


Ala Glu Lys Glu Gly Tyr Glu Val Arg Ile Leu Thr Ala Asp Lys Asp 
    130                 135                 140                 


Leu Tyr Gln Leu Leu Ser Asp Arg Ile His Val Leu His Pro Glu Gly 
145                 150                 155                 160 


Tyr Leu Ile Thr Pro Ala Trp Leu Trp Glu Lys Tyr Gly Leu Arg Pro 
                165                 170                 175     


Asp Gln Trp Ala Asp Tyr Arg Ala Leu Thr Gly Asp Glu Ser Asp Asn 
            180                 185                 190         


Leu Pro Gly Val Lys Gly Ile Gly Glu Lys Thr Ala Arg Lys Leu Leu 
        195                 200                 205             


Glu Glu Trp Gly Ser Leu Glu Ala Leu Leu Lys Asn Leu Asp Arg Leu 
    210                 215                 220                 


Lys Pro Ala Ile Arg Glu Lys Ile Leu Ala His Met Asp Asp Leu Lys 
225                 230                 235                 240 


Leu Ser Trp Asp Leu Ala Lys Val Arg Thr Asp Leu Pro Leu Glu Val 
                245                 250                 255     


Asp Phe Ala Lys Arg Arg Glu Pro Asp Arg Glu Arg Leu Arg Ala Phe 
            260                 265                 270         


Leu Glu Arg Leu Glu Phe Gly Ser Leu Leu His Glu Phe Gly Leu Leu 
        275                 280                 285             


Glu Ser Pro Lys Ala Leu Glu Glu Ala Pro Trp Pro Pro Pro Glu Gly 
    290                 295                 300                 


Ala Phe Val Gly Phe Val Leu Ser Arg Lys Glu Pro Met Trp Ala Asp 
305                 310                 315                 320 


Leu Leu Ala Leu Ala Ala Ala Arg Gly Gly Arg Val His Arg Ala Pro 
                325                 330                 335     


Glu Pro Tyr Lys Ala Leu Arg Asp Leu Lys Glu Ala Arg Gly Leu Leu 
            340                 345                 350         


Ala Lys Asp Leu Ser Val Leu Ala Leu Arg Glu Gly Leu Gly Leu Pro 
        355                 360                 365             


Pro Gly Asp Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro Ser Asn 
    370                 375                 380                 


Thr Thr Pro Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp Thr Glu 
385                 390                 395                 400 


Glu Ala Gly Glu Arg Ala Ala Leu Ser Glu Arg Leu Phe Ala Asn Leu 
                405                 410                 415     


Trp Gly Arg Leu Glu Gly Glu Glu Arg Leu Leu Trp Leu Tyr Arg Glu 
            420                 425                 430         


Val Glu Arg Pro Leu Ser Ala Val Leu Ala His Met Glu Ala Thr Gly 
        435                 440                 445             


Val Arg Leu Asp Val Ala Tyr Leu Arg Ala Leu Ser Leu Glu Val Ala 
    450                 455                 460                 


Glu Glu Ile Ala Arg Leu Glu Ala Glu Val Phe Arg Leu Ala Gly His 
465                 470                 475                 480 


Pro Phe Asn Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu Phe Asp 
                485                 490                 495     


Glu Leu Gly Leu Pro Ala Ile Gly Lys Thr Glu Lys Thr Gly Lys Arg 
            500                 505                 510         


Ser Thr Ser Ala Ala Val Leu Glu Ala Leu Arg Glu Ala His Pro Ile 
        515                 520                 525             


Val Glu Lys Ile Leu Gln Tyr Arg Glu Leu Thr Lys Leu Lys Ser Thr 
    530                 535                 540                 


Tyr Ile Asp Pro Leu Pro Asp Leu Ile His Pro Arg Thr Gly Arg Leu 
545                 550                 555                 560 


His Thr Arg Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu Ser Ser 
                565                 570                 575     


Ser Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Thr Pro Leu Gly Gln 
            580                 585                 590         


Arg Ile Arg Arg Ala Phe Ile Ala Glu Glu Gly Trp Leu Leu Val Ala 
        595                 600                 605             


Leu Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu Ser Gly 
    610                 615                 620                 


Asp Glu Asn Leu Ile Arg Val Phe Gln Glu Gly Arg Asp Ile His Thr 
625                 630                 635                 640 


Glu Thr Ala Ser Trp Met Phe Gly Val Pro Arg Glu Ala Val Asp Pro 
                645                 650                 655     


Leu Met Arg Arg Ala Ala Lys Thr Ile Asn Phe Gly Val Leu Tyr Gly 
            660                 665                 670         


Met Ser Ala His Arg Leu Ser Gln Glu Leu Ala Ile Pro Tyr Glu Glu 
        675                 680                 685             


Ala Gln Ala Phe Ile Glu Arg Tyr Phe Gln Ser Phe Pro Lys Val Arg 
    690                 695                 700                 


Ala Trp Ile Glu Lys Thr Leu Glu Glu Gly Arg Arg Arg Gly Tyr Val 
705                 710                 715                 720 


Glu Thr Leu Phe Gly Arg Arg Arg Tyr Val Pro Asp Leu Glu Ala Arg 
                725                 730                 735     


Val Lys Ser Val Arg Arg Arg Ala Glu Arg Met Ala Phe Asn Met Pro 
            740                 745                 750         


Val Gln Gly Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val Lys Leu 
        755                 760                 765             


Phe Pro Arg Leu Glu Glu Met Gly Ala Arg Met Leu Leu Gln Val His 
    770                 775                 780                 


Asp Glu Leu Val Leu Glu Ala Pro Lys Glu Arg Ala Glu Ala Val Ala 
785                 790                 795                 800 


Arg Leu Ala Lys Glu Val Met Glu Gly Val Tyr Pro Leu Ala Val Pro 
                805                 810                 815     


Leu Glu Val Glu Val Gly Ile Gly Glu Asp Trp Leu Ser Ala Lys Glu 
            820                 825                 830         


<210>  6
<211>  554
<212>  PRT
<213>  Thermus aquaticus

<400>  6

Met Gly Leu Leu His Glu Phe Gly Leu Leu Glu Ser Pro Lys Ala Leu 
1               5                   10                  15      


Glu Glu Ala Pro Trp Pro Pro Pro Glu Gly Ala Phe Val Gly Phe Val 
            20                  25                  30          


Leu Ser Arg Lys Glu Pro Met Trp Ala Asp Leu Leu Ala Leu Ala Ala 
        35                  40                  45              


Ala Arg Gly Gly Arg Val His Arg Ala Pro Glu Pro Tyr Lys Ala Leu 
    50                  55                  60                  


Arg Asp Leu Lys Glu Ala Arg Gly Leu Leu Ala Lys Asp Leu Ser Val 
65                  70                  75                  80  


Leu Ala Leu Arg Glu Gly Leu Gly Leu Pro Pro Gly Asp Asp Pro Met 
                85                  90                  95      


Leu Leu Ala Tyr Leu Leu Asp Pro Ser Asn Thr Thr Pro Glu Gly Val 
            100                 105                 110         


Ala Arg Arg Tyr Gly Gly Glu Trp Thr Glu Glu Ala Gly Glu Arg Ala 
        115                 120                 125             


Ala Leu Ser Glu Arg Leu Phe Ala Asn Leu Trp Gly Arg Leu Glu Gly 
    130                 135                 140                 


Glu Glu Arg Leu Leu Trp Leu Tyr Arg Glu Val Glu Arg Pro Leu Ser 
145                 150                 155                 160 


Ala Val Leu Ala His Met Glu Ala Thr Gly Val Arg Leu Asp Val Ala 
                165                 170                 175     


Tyr Leu Arg Ala Leu Ser Leu Glu Val Ala Glu Glu Ile Ala Arg Leu 
            180                 185                 190         


Glu Ala Glu Val Phe Arg Leu Ala Gly His Pro Phe Asn Leu Asn Ser 
        195                 200                 205             


Arg Asp Gln Leu Glu Arg Val Leu Phe Asp Glu Leu Gly Leu Pro Ala 
    210                 215                 220                 


Ile Gly Lys Thr Glu Lys Thr Gly Lys Arg Ser Thr Ser Ala Ala Val 
225                 230                 235                 240 


Leu Glu Ala Leu Arg Glu Ala His Pro Ile Val Glu Lys Ile Leu Gln 
                245                 250                 255     


Tyr Arg Glu Leu Thr Lys Leu Lys Ser Thr Tyr Ile Asp Pro Leu Pro 
            260                 265                 270         


Asp Leu Ile His Pro Arg Thr Gly Arg Leu His Thr Arg Phe Asn Gln 
        275                 280                 285             


Thr Ala Thr Ala Thr Gly Arg Leu Ser Ser Ser Asp Pro Asn Leu Gln 
    290                 295                 300                 


Asn Ile Pro Val Arg Thr Pro Leu Gly Gln Arg Ile Arg Arg Ala Phe 
305                 310                 315                 320 


Ile Ala Glu Glu Gly Trp Leu Leu Val Ala Leu Asp Tyr Ser Gln Ile 
                325                 330                 335     


Glu Leu Arg Val Leu Ala His Leu Ser Gly Asp Lys Asn Leu Ile Arg 
            340                 345                 350         


Val Phe Gln Glu Gly Arg Asp Ile His Thr Glu Thr Ala Ser Trp Met 
        355                 360                 365             


Phe Gly Val Pro Arg Glu Ala Val Asp Pro Leu Met Arg Arg Ala Ala 
    370                 375                 380                 


Lys Thr Ile Asn Phe Gly Val Leu Tyr Gly Met Ser Ala His Arg Leu 
385                 390                 395                 400 


Ser Gln Glu Leu Ala Ile Pro Tyr Glu Glu Ala Gln Ala Phe Ile Glu 
                405                 410                 415     


Arg Tyr Phe Gln Ser Phe Pro Lys Val Arg Ala Trp Leu Lys Lys Thr 
            420                 425                 430         


Leu Glu Glu Gly Arg Arg Arg Gly Tyr Val Glu Thr Leu Phe Gly Arg 
        435                 440                 445             


Arg Arg Tyr Val Pro Asp Leu Glu Ala Arg Val Lys Ser Val Arg Arg 
    450                 455                 460                 


Arg Ala Glu Arg Met Ala Phe Asn Met Pro Val Gln Gly Thr Ala Ala 
465                 470                 475                 480 


Asp Leu Met Lys Leu Ala Met Val Lys Leu Phe Pro Arg Leu Glu Glu 
                485                 490                 495     


Met Gly Ala Arg Met Leu Leu Gln Val His Asp Glu Leu Val Leu Glu 
            500                 505                 510         


Ala Pro Lys Glu Arg Ala Glu Ala Val Ala Arg Leu Ala Lys Glu Val 
        515                 520                 525             


Met Glu Gly Val Tyr Pro Leu Ala Val Pro Leu Glu Val Glu Val Gly 
    530                 535                 540                 


Ile Gly Glu Asp Trp Leu Ser Ala Lys Glu 
545                 550                 


<210>  7
<211>  832
<212>  PRT
<213>  Thermus aquaticus

<400>  7

Met Arg Gly Met Leu Pro Leu Phe Glu Pro Lys Gly Arg Val Leu Leu 
1               5                   10                  15      


Val Asp Gly His His Leu Ala Tyr Arg Thr Phe His Ala Leu Lys Gly 
            20                  25                  30          


Leu Thr Thr Ser Arg Gly Glu Pro Val Gln Ala Val Tyr Gly Phe Ala 
        35                  40                  45              


Lys Ser Leu Leu Lys Ala Leu Lys Glu Asp Gly Asp Ala Val Ile Val 
    50                  55                  60                  


Val Phe Asp Ala Lys Ala Pro Ser Phe Arg His Glu Ala Tyr Gly Gly 
65                  70                  75                  80  


Tyr Lys Ala Gly Arg Ala Pro Thr Pro Glu Asp Phe Pro Arg Gln Leu 
                85                  90                  95      


Ala Leu Ile Lys Glu Leu Val Asp Leu Leu Gly Leu Ala Arg Leu Glu 
            100                 105                 110         


Val Pro Gly Tyr Glu Ala Asp Asp Val Leu Ala Ser Leu Ala Lys Lys 
        115                 120                 125             


Ala Glu Lys Glu Gly Tyr Glu Val Arg Ile Leu Thr Ala Asp Lys Asp 
    130                 135                 140                 


Leu Tyr Gln Leu Leu Ser Asp Arg Ile His Val Leu His Pro Glu Gly 
145                 150                 155                 160 


Tyr Leu Ile Thr Pro Ala Trp Leu Trp Glu Lys Tyr Gly Leu Arg Pro 
                165                 170                 175     


Asp Gln Trp Ala Asp Tyr Arg Ala Leu Thr Gly Asp Glu Ser Asp Asn 
            180                 185                 190         


Leu Pro Gly Val Lys Gly Ile Gly Glu Lys Thr Ala Arg Lys Leu Leu 
        195                 200                 205             


Glu Glu Trp Gly Ser Leu Glu Ala Leu Leu Lys Asn Leu Asp Arg Leu 
    210                 215                 220                 


Lys Pro Ala Ile Arg Glu Lys Ile Leu Ala His Met Asp Asp Leu Lys 
225                 230                 235                 240 


Leu Ser Trp Asp Leu Ala Lys Val Arg Thr Asp Leu Pro Leu Glu Val 
                245                 250                 255     


Asp Phe Ala Lys Arg Arg Glu Pro Asp Arg Glu Arg Leu Arg Ala Phe 
            260                 265                 270         


Leu Glu Arg Leu Glu Phe Gly Ser Leu Leu His Glu Phe Gly Leu Leu 
        275                 280                 285             


Glu Ser Pro Lys Ala Leu Glu Glu Ala Pro Trp Pro Pro Pro Glu Gly 
    290                 295                 300                 


Ala Phe Val Gly Phe Val Leu Ser Arg Lys Glu Pro Met Trp Ala Asp 
305                 310                 315                 320 


Leu Leu Ala Leu Ala Ala Ala Arg Gly Gly Arg Val His Arg Ala Pro 
                325                 330                 335     


Glu Pro Tyr Lys Ala Leu Arg Asp Leu Lys Glu Ala Arg Gly Leu Leu 
            340                 345                 350         


Ala Lys Asp Leu Ser Val Leu Ala Leu Arg Glu Gly Leu Gly Leu Pro 
        355                 360                 365             


Pro Gly Asp Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro Ser Asn 
    370                 375                 380                 


Thr Thr Pro Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp Thr Glu 
385                 390                 395                 400 


Glu Ala Gly Glu Arg Ala Ala Leu Ser Glu Arg Leu Phe Ala Asn Leu 
                405                 410                 415     


Trp Gly Arg Leu Glu Gly Glu Glu Arg Leu Leu Trp Leu Tyr Arg Glu 
            420                 425                 430         


Val Glu Arg Pro Leu Ser Ala Val Leu Ala His Met Glu Ala Thr Gly 
        435                 440                 445             


Val Arg Leu Asp Val Ala Tyr Leu Arg Ala Leu Ser Leu Glu Val Ala 
    450                 455                 460                 


Glu Glu Ile Ala Arg Leu Glu Ala Glu Val Phe Arg Leu Ala Gly His 
465                 470                 475                 480 


Pro Phe Asn Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu Phe Asp 
                485                 490                 495     


Glu Leu Gly Leu Pro Ala Ile Gly Lys Thr Glu Lys Thr Gly Lys Arg 
            500                 505                 510         


Ser Thr Ser Ala Ala Val Leu Glu Ala Leu Arg Glu Ala His Pro Ile 
        515                 520                 525             


Val Glu Lys Ile Leu Gln Tyr Arg Glu Leu Thr Lys Leu Lys Ser Thr 
    530                 535                 540                 


Tyr Ile Asp Pro Leu Pro Asp Leu Ile His Pro Arg Thr Gly Arg Leu 
545                 550                 555                 560 


His Thr Arg Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu Ser Ser 
                565                 570                 575     


Ser Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Thr Pro Leu Gly Gln 
            580                 585                 590         


Arg Ile Arg Arg Ala Phe Ile Ala Glu Glu Gly Trp Leu Leu Val Ala 
        595                 600                 605             


Leu Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu Ser Gly 
    610                 615                 620                 


Asp Glu Asn Leu Ile Arg Val Phe Gln Glu Gly Arg Asp Ile His Thr 
625                 630                 635                 640 


Glu Thr Ala Ser Trp Met Phe Gly Val Pro Arg Glu Ala Val Asp Pro 
                645                 650                 655     


Leu Met Arg Arg Ala Ala Lys Thr Ile Asn Phe Gly Val Leu Tyr Gly 
            660                 665                 670         


Met Ser Ala His Arg Leu Ser Gln Glu Leu Ala Ile Pro Tyr Glu Glu 
        675                 680                 685             


Ala Gln Ala Phe Ile Glu Arg Tyr Phe Gln Ser Phe Pro Lys Val Arg 
    690                 695                 700                 


Ala Trp Ile Glu Lys Thr Leu Glu Glu Gly Arg Arg Arg Gly Tyr Val 
705                 710                 715                 720 


Glu Thr Leu Phe Gly Arg Arg Arg Tyr Val Pro Asn Leu Glu Ala Arg 
                725                 730                 735     


Val Lys Ser Val Arg Arg Arg Ala Glu Arg Met Ala Phe Asn Met Pro 
            740                 745                 750         


Val Gln Gly Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val Lys Leu 
        755                 760                 765             


Phe Pro Arg Leu Glu Glu Met Gly Ala Arg Met Leu Leu Gln Val His 
    770                 775                 780                 


Asp Glu Leu Val Leu Glu Ala Pro Lys Glu Arg Ala Glu Ala Val Ala 
785                 790                 795                 800 


Arg Leu Ala Lys Glu Val Met Glu Gly Val Tyr Pro Leu Ala Val Pro 
                805                 810                 815     


Leu Glu Val Glu Val Gly Ile Gly Glu Asp Trp Leu Ser Ala Lys Glu 
            820                 825                 830         


<210>  8
<211>  554
<212>  PRT
<213>  Thermus aquaticus

<400>  8

Met Gly Leu Leu His Glu Phe Gly Leu Leu Glu Ser Pro Lys Ala Leu 
1               5                   10                  15      


Glu Glu Ala Pro Trp Pro Pro Pro Glu Gly Ala Phe Val Gly Phe Val 
            20                  25                  30          


Leu Ser Arg Lys Glu Pro Met Trp Ala Asp Leu Leu Ala Leu Ala Ala 
        35                  40                  45              


Ala Arg Gly Gly Arg Val His Arg Ala Pro Glu Pro Tyr Lys Ala Leu 
    50                  55                  60                  


Arg Asp Leu Lys Glu Ala Arg Gly Leu Leu Ala Lys Asp Leu Ser Val 
65                  70                  75                  80  


Leu Ala Leu Arg Glu Gly Leu Gly Leu Pro Pro Gly Asp Asp Pro Met 
                85                  90                  95      


Leu Leu Ala Tyr Leu Leu Asp Pro Ser Asn Thr Thr Pro Glu Gly Val 
            100                 105                 110         


Ala Arg Arg Tyr Gly Gly Glu Trp Thr Glu Glu Ala Gly Glu Arg Ala 
        115                 120                 125             


Ala Leu Ser Glu Arg Leu Phe Ala Asn Leu Trp Gly Arg Leu Glu Gly 
    130                 135                 140                 


Glu Glu Arg Leu Leu Trp Leu Tyr Arg Glu Val Glu Arg Pro Leu Ser 
145                 150                 155                 160 


Ala Val Leu Ala His Met Glu Ala Thr Gly Val Arg Leu Asp Val Ala 
                165                 170                 175     


Tyr Leu Arg Ala Leu Ser Leu Glu Val Ala Glu Glu Ile Ala Arg Leu 
            180                 185                 190         


Glu Ala Glu Val Phe Arg Leu Ala Gly His Pro Phe Asn Leu Asn Ser 
        195                 200                 205             


Arg Asp Gln Leu Glu Arg Val Leu Phe Asp Glu Leu Gly Leu Pro Ala 
    210                 215                 220                 


Ile Gly Lys Thr Glu Lys Thr Gly Lys Arg Ser Thr Ser Ala Ala Val 
225                 230                 235                 240 


Leu Glu Ala Leu Arg Glu Ala His Pro Ile Val Glu Lys Ile Leu Gln 
                245                 250                 255     


Tyr Arg Glu Leu Thr Lys Leu Lys Ser Thr Tyr Ile Asp Pro Leu Pro 
            260                 265                 270         


Asp Leu Ile His Pro Arg Thr Gly Arg Leu His Thr Arg Phe Asn Gln 
        275                 280                 285             


Thr Ala Thr Ala Thr Gly Arg Leu Ser Ser Ser Asp Pro Asn Leu Gln 
    290                 295                 300                 


Asn Ile Pro Val Arg Thr Pro Leu Gly Gln Arg Ile Arg Arg Ala Phe 
305                 310                 315                 320 


Ile Ala Glu Glu Gly Trp Leu Leu Val Ala Leu Asp Tyr Ser Gln Ile 
                325                 330                 335     


Glu Leu Arg Val Leu Ala His Leu Ser Gly Asp Lys Asn Leu Ile Arg 
            340                 345                 350         


Val Phe Gln Glu Gly Arg Asp Ile His Thr Glu Thr Ala Ser Trp Met 
        355                 360                 365             


Phe Gly Val Pro Arg Glu Ala Val Asp Pro Leu Met Arg Arg Ala Ala 
    370                 375                 380                 


Lys Thr Ile Asn Phe Gly Val Leu Tyr Gly Met Ser Ala His Arg Leu 
385                 390                 395                 400 


Ser Gln Glu Leu Ala Ile Pro Tyr Glu Glu Ala Gln Ala Phe Ile Glu 
                405                 410                 415     


Arg Tyr Phe Gln Ser Phe Pro Lys Val Arg Ala Trp Leu Lys Lys Thr 
            420                 425                 430         


Leu Glu Glu Gly Arg Arg Arg Gly Tyr Val Glu Thr Leu Phe Gly Arg 
        435                 440                 445             


Arg Arg Tyr Val Pro Asn Leu Glu Ala Arg Val Lys Ser Val Arg Arg 
    450                 455                 460                 


Arg Ala Glu Arg Met Ala Phe Asn Met Pro Val Gln Gly Thr Ala Ala 
465                 470                 475                 480 


Asp Leu Met Lys Leu Ala Met Val Lys Leu Phe Pro Arg Leu Glu Glu 
                485                 490                 495     


Met Gly Ala Arg Met Leu Leu Gln Val His Asp Glu Leu Val Leu Glu 
            500                 505                 510         


Ala Pro Lys Glu Arg Ala Glu Ala Val Ala Arg Leu Ala Lys Glu Val 
        515                 520                 525             


Met Glu Gly Val Tyr Pro Leu Ala Val Pro Leu Glu Val Glu Val Gly 
    530                 535                 540                 


Ile Gly Glu Asp Trp Leu Ser Ala Lys Glu 
545                 550                 


<210>  9
<211>  563
<212>  PRT
<213>  Thermus aquaticus

<400>  9

Met Gly Leu Leu His Glu Phe Gly Leu Leu Glu Ser Pro Lys Ala Leu 
1               5                   10                  15      


Glu Glu Ala Pro Trp Pro Pro Pro Glu Gly Ala Phe Val Gly Phe Val 
            20                  25                  30          


Leu Ser Arg Lys Glu Pro Met Trp Ala Asp Leu Leu Ala Leu Ala Ala 
        35                  40                  45              


Ala Arg Gly Gly Arg Val His Arg Ala Pro Glu Pro Tyr Lys Ala Leu 
    50                  55                  60                  


Arg Asp Leu Lys Glu Ala Arg Gly Leu Leu Ala Lys Asp Leu Ser Val 
65                  70                  75                  80  


Leu Ala Leu Arg Glu Gly Leu Gly Leu Pro Pro Gly Asp Asp Pro Met 
                85                  90                  95      


Leu Leu Ala Tyr Leu Leu Asp Pro Ser Asn Thr Thr Pro Glu Gly Val 
            100                 105                 110         


Ala Arg Arg Tyr Gly Gly Glu Trp Thr Glu Glu Ala Gly Glu Arg Ala 
        115                 120                 125             


Ala Leu Ser Glu Arg Leu Phe Ala Asn Leu Trp Gly Arg Leu Glu Gly 
    130                 135                 140                 


Glu Glu Arg Leu Leu Trp Leu Tyr Arg Glu Val Glu Arg Pro Leu Ser 
145                 150                 155                 160 


Ala Val Leu Ala His Met Glu Ala Thr Gly Val Arg Leu Asp Val Ala 
                165                 170                 175     


Tyr Leu Arg Ala Leu Ser Leu Glu Val Ala Glu Glu Ile Ala Arg Leu 
            180                 185                 190         


Glu Ala Glu Val Phe Arg Leu Ala Gly His Pro Phe Asn Leu Asn Ser 
        195                 200                 205             


Arg Asp Gln Leu Glu Arg Val Leu Phe Asp Glu Leu Gly Leu Pro Ala 
    210                 215                 220                 


Ile Gly Lys Thr Glu Lys Thr Gly Lys Arg Ser Thr Ser Ala Ala Val 
225                 230                 235                 240 


Leu Glu Ala Leu Arg Glu Ala His Pro Ile Val Glu Lys Ile Leu Gln 
                245                 250                 255     


Tyr Arg Glu Leu Thr Lys Leu Lys Ser Thr Tyr Ile Asp Pro Leu Pro 
            260                 265                 270         


Asp Leu Ile His Pro Arg Thr Gly Arg Leu His Thr Arg Phe Asn Gln 
        275                 280                 285             


Thr Ala Thr Ala Thr Gly Arg Leu Ser Ser Ser Asp Pro Asn Leu Gln 
    290                 295                 300                 


Asn Ile Pro Val Arg Thr Pro Leu Gly Gln Arg Ile Arg Arg Ala Phe 
305                 310                 315                 320 


Ile Ala Glu Glu Gly Trp Leu Leu Val Ala Leu Asp Tyr Ser Gln Ile 
                325                 330                 335     


Glu Leu Arg Val Leu Ala His Leu Ser Gly Asp Lys Asn Leu Ile Arg 
            340                 345                 350         


Val Phe Gln Glu Gly Arg Asp Ile His Thr Glu Thr Ala Ser Trp Met 
        355                 360                 365             


Phe Gly Val Pro Arg Glu Ala Val Asp Pro Leu Met Arg Arg Ala Ala 
    370                 375                 380                 


Lys Thr Ile Asn Phe Gly Val Leu Tyr Gly Met Ser Ala His Arg Leu 
385                 390                 395                 400 


Ser Gln Glu Leu Ala Ile Pro Tyr Glu Glu Ala Gln Ala Phe Ile Glu 
                405                 410                 415     


Arg Tyr Phe Gln Ser Phe Pro Lys Val Arg Ala Trp Leu Lys Lys Thr 
            420                 425                 430         


Leu Glu Glu Gly Arg Arg Arg Gly Tyr Val Glu Thr Leu Phe Gly Arg 
        435                 440                 445             


Arg Arg Tyr Val Pro Asn Leu Glu Ala Arg Val Lys Phe Leu Asn Cys 
    450                 455                 460                 


Cys Pro Gly Cys Cys Ser Val Arg Arg Arg Ala Glu Arg Met Ala Phe 
465                 470                 475                 480 


Asn Met Pro Val Gln Gly Thr Ala Ala Asp Leu Met Lys Leu Ala Met 
                485                 490                 495     


Val Lys Leu Phe Pro Arg Leu Glu Glu Met Gly Ala Arg Met Leu Leu 
            500                 505                 510         


Gln Val His Asp Glu Leu Val Leu Glu Ala Pro Lys Glu Arg Ala Glu 
        515                 520                 525             


Ala Val Ala Arg Leu Ala Lys Glu Val Met Glu Gly Val Tyr Pro Leu 
    530                 535                 540                 


Ala Val Pro Leu Glu Val Glu Val Gly Ile Gly Glu Asp Trp Leu Ser 
545                 550                 555                 560 


Ala Lys Glu 
            


<210>  10
<211>  581
<212>  PRT
<213>  Bacillus stearothermophilus

<400>  10

Met Ala Lys Met Ala Phe Thr Leu Ala Asp Arg Val Thr Glu Glu Met 
1               5                   10                  15      


Leu Ala Asp Lys Ala Ala Leu Val Val Glu Val Val Glu Glu Asn Tyr 
            20                  25                  30          


His Asp Ala Pro Ile Val Gly Ile Ala Val Val Asn Glu His Gly Arg 
        35                  40                  45              


Phe Phe Leu Arg Pro Glu Thr Ala Leu Ala Asp Pro Gln Phe Val Ala 
    50                  55                  60                  


Trp Leu Gly Asp Glu Thr Lys Lys Lys Ser Met Phe Asp Ser Lys Arg 
65                  70                  75                  80  


Ala Ala Val Ala Leu Lys Trp Lys Gly Ile Glu Leu Cys Gly Val Ser 
                85                  90                  95      


Phe Asp Leu Leu Leu Ala Ala Tyr Leu Leu Asp Pro Ala Gln Gly Val 
            100                 105                 110         


Asp Asp Val Ala Ala Ala Ala Lys Met Lys Gln Tyr Glu Ala Val Arg 
        115                 120                 125             


Pro Asp Glu Ala Val Tyr Gly Lys Gly Ala Lys Arg Ala Val Pro Asp 
    130                 135                 140                 


Glu Pro Val Leu Ala Glu His Leu Val Arg Lys Ala Ala Ala Ile Trp 
145                 150                 155                 160 


Glu Leu Glu Arg Pro Phe Leu Asp Glu Leu Arg Arg Asn Glu Gln Asp 
                165                 170                 175     


Arg Leu Leu Val Glu Leu Glu Gln Pro Leu Ser Ser Ile Leu Ala Glu 
            180                 185                 190         


Met Glu Phe Ala Gly Val Lys Val Asp Thr Lys Arg Leu Glu Gln Met 
        195                 200                 205             


Gly Lys Glu Leu Ala Glu Gln Leu Gly Thr Val Glu Gln Arg Ile Tyr 
    210                 215                 220                 


Glu Leu Ala Gly Gln Glu Phe Asn Ile Asn Ser Pro Lys Gln Leu Gly 
225                 230                 235                 240 


Val Ile Leu Phe Glu Lys Leu Gln Leu Pro Val Leu Lys Lys Thr Lys 
                245                 250                 255     


Thr Gly Tyr Ser Thr Ser Ala Asp Val Leu Glu Lys Leu Ala Pro Tyr 
            260                 265                 270         


His Glu Ile Val Glu Asn Ile Leu His Tyr Arg Gln Leu Gly Lys Leu 
        275                 280                 285             


Gln Ser Thr Tyr Ile Glu Gly Leu Leu Lys Val Val Arg Pro Asp Thr 
    290                 295                 300                 


Lys Lys Val His Thr Ile Phe Asn Gln Ala Leu Thr Gln Thr Gly Arg 
305                 310                 315                 320 


Leu Ser Ser Thr Glu Pro Asn Leu Gln Asn Ile Pro Ile Arg Leu Glu 
                325                 330                 335     


Glu Gly Arg Lys Ile Arg Gln Ala Phe Val Pro Ser Glu Ser Asp Trp 
            340                 345                 350         


Leu Ile Phe Ala Ala Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala 
        355                 360                 365             


His Ile Ala Glu Asp Asp Asn Leu Met Glu Ala Phe Arg Arg Asp Leu 
    370                 375                 380                 


Asp Ile His Thr Lys Thr Ala Met Asp Ile Phe Gln Val Ser Glu Asp 
385                 390                 395                 400 


Glu Val Thr Pro Asn Met Arg Arg Gln Ala Lys Ala Val Asn Phe Gly 
                405                 410                 415     


Ile Val Tyr Gly Ile Ser Asp Tyr Gly Leu Ala Gln Asn Leu Asn Ile 
            420                 425                 430         


Ser Arg Lys Glu Ala Ala Glu Phe Ile Glu Arg Tyr Phe Glu Ser Phe 
        435                 440                 445             


Pro Gly Val Lys Arg Tyr Met Glu Asn Ile Val Gln Glu Ala Lys Gln 
    450                 455                 460                 


Lys Gly Tyr Val Thr Thr Leu Leu His Arg Arg Arg Tyr Leu Pro Asp 
465                 470                 475                 480 


Ile Thr Ser Arg Asn Phe Asn Val Arg Ser Phe Ala Glu Arg Met Ala 
                485                 490                 495     


Met Asn Thr Pro Ile Gln Gly Ser Ala Ala Asp Ile Ile Lys Lys Ala 
            500                 505                 510         


Met Ile Asp Leu Asn Ala Arg Leu Lys Glu Glu Arg Leu Gln Ala His 
        515                 520                 525             


Leu Leu Leu Gln Val His Asp Glu Leu Ile Leu Glu Ala Pro Lys Glu 
    530                 535                 540                 


Glu Met Glu Arg Leu Cys Arg Leu Val Pro Glu Val Met Glu Gln Ala 
545                 550                 555                 560 


Val Thr Leu Arg Val Pro Leu Lys Val Asp Tyr His Tyr Gly Ser Thr 
                565                 570                 575     


Trp Tyr Asp Ala Lys 
            580     


<210>  11
<211>  31
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR Primer

<400>  11
tacagacgtt taagcttcgc aattaccggt t                                      31


<210>  12
<211>  30
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR Primer

<400>  12
aaaaagctgc aaattgcggt aggtattatt                                        30


<210>  13
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR Primer

<400>  13
ctaccaaggt aatgaagcaa atggatat                                          28


<210>  14
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR Primer

<400>  14
tggcagcaac caaagtatat gaaaggg                                           27


<210>  15
<211>  26
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR Primer

<400>  15
ggcattgttt ggtaggtgag agatct                                            26


<210>  16
<211>  30
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR Primer

<400>  16
acaaatgaca agagtctggt tcagaagata                                        30


<210>  17
<211>  74
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Stem-loop

<400>  17
agctctgtct cttatacaca tctaatagtt taactttaag aaggagatat aaataagatg       60

tgtataagag acag                                                         74


<210>  18
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  RT-LAMP Primer

<400>  18
tgtcatggga tccggatgtt                                                   20


<210>  19
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  RT-LAMP Primer

<400>  19
caatagagcc gctctcagag                                                   20


<210>  20
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  RT-LAMP Primer

<400>  20
ccagagagga ggttgccaa                                                    19


<210>  21
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  RT-LAMP Primer

<400>  21
tgcaggatgc agcgcctta                                                    19


<210>  22
<211>  46
<212>  DNA
<213>  Artificial sequence

<220>
<223>  RT-LAMP Primer

<400>  22
gcccaaacaa cgacgatcgg tagagtcaaa ccagcatccg tagcct                      46


<210>  23
<211>  45
<212>  DNA
<213>  Artificial sequence

<220>
<223>  RT-LAMP Primer

<400>  23
gcacgttctc caacggtgct gagtcggttg cttgttcagc gaact                       45


<210>  24
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR Primer

<400>  24
agggtgcata tgagatgctt ac                                                22


<210>  25
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR Primer

<400>  25
agatacctag agacgacaac ca                                                22


<210>  26
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR Primer

<400>  26
gatcgtatcc gctcacacta c                                                 21


<210>  27
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR Primer

<400>  27
tgcaatctca ctgggacata taa                                               23


<210>  28
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  RT-LAMP Primer

<400>  28
gtaaaaacac ctcacgagtt                                                   20


<210>  29
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  RT-LAMP Primer

<400>  29
tttacgaaca ttaagcgact t                                                 21


<210>  30
<211>  45
<212>  DNA
<213>  Artificial sequence

<220>
<223>  RT-LAMP Primer

<400>  30
tcctacggtc aagagaagca ataaacacct aagttctcac cgaat                       45


<210>  31
<211>  40
<212>  DNA
<213>  Artificial sequence

<220>
<223>  RT-LAMP Primer

<400>  31
ctttccacat gcaggatttt ggatgcacgc aatggtgtag                             40


<210>  32
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  F1AsH insertion peptide

<400>  32

Phe Leu Asn Cys Cys Pro Gly Cys Cys 
1               5                   


