                         SEQUENCE LISTING

<110>  Regeneron Pharmaceuticals, Inc.
 
<120>  Multimeric Protein Purity Determination

<130>  10141US01

<160>  20    

<170>  PatentIn version 3.5

<210>  1
<211>  12
<212>  PRT
<213>  artificial

<220>
<223>  synthetic

<400>  1

Tyr Thr Phe Thr Asn Tyr Trp Ile His Trp Glu Lys 
1               5                   10          


<210>  2
<211>  10
<212>  PRT
<213>  artificial

<220>
<223>  synthetic

<400>  2

Gly His Thr Glu Tyr Asn Gln Lys Phe Lys 
1               5                   10  


<210>  3
<211>  13
<212>  PRT
<213>  artificial

<220>
<223>  synthetic

<400>  3

His Phe Asp Tyr Trp Gly Gln Gly Thr Thr Leu Ile Val 
1               5                   10              


<210>  4
<211>  12
<212>  PRT
<213>  artificial

<220>
<223>  synthetic

<400>  4

Gln Ser Leu Val His Asn Asn Gly Asp Thr Phe Leu 
1               5                   10          


<210>  5
<211>  10
<212>  PRT
<213>  artificial

<220>
<223>  synthetic

<400>  5

Val Ser Asn Arg Phe Ser Gly Val Pro Asp 
1               5                   10  


<210>  6
<211>  13
<212>  PRT
<213>  artificial

<220>
<223>  synthetic

<400>  6

Gly Gly Thr Lys Leu Glu Ile Lys Arg Ala Asp Ala Ala 
1               5                   10              


<210>  7
<211>  12
<212>  PRT
<213>  artificial

<220>
<223>  Synthetic

<400>  7

Phe Thr Phe Asn Asn Tyr Ala Met Asn Trp Val Arg 
1               5                   10          


<210>  8
<211>  10
<212>  PRT
<213>  artificial

<220>
<223>  synthetic

<400>  8

Gly Asn Thr Tyr His Ala Asp Ser Val Lys 
1               5                   10  


<210>  9
<211>  13
<212>  PRT
<213>  artificial

<220>
<223>  synthetic

<400>  9

Gly Ala Phe Asp Ile Trp Gly Gln Gly Thr Met Val Thr 
1               5                   10              


<210>  10
<211>  12
<212>  PRT
<213>  artificial

<220>
<223>  synthetic

<400>  10

Gln Ser Val Phe Tyr Ser Ser Asn Lys Gln Asn Phe 
1               5                   10          


<210>  11
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  synthetic

<400>  11

Arg Ala Ser Thr Arg Glu Ser Gly Val 
1               5                   


<210>  12
<211>  13
<212>  PRT
<213>  artificial

<220>
<223>  synthetic

<400>  12

Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Ala Asp Ala 
1               5                   10              


<210>  13
<211>  128
<212>  PRT
<213>  artificial

<220>
<223>  synthetic

<400>  13

Glu Val Gln Leu Val Glu Ser Gly Gly Asp Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn Asn Tyr 
            20                  25                  30          


Ala Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Gly Ser Ser Gly Ser Gly Gly Asn Thr Tyr His Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Asn Asn Ala Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Lys Gln Gly Asp Trp Asn Trp Gly Ala Phe Asp Ile Trp Gly Gln 
            100                 105                 110         


Gly Thr Met Val Thr Val Ser Ser Ala Lys Thr Thr Ala Pro Ser Val 
        115                 120                 125             


<210>  14
<211>  128
<212>  PRT
<213>  artificial

<220>
<223>  synthetic

<400>  14

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Arg Ser Ser Gln Ser Val Phe Tyr Ser 
            20                  25                  30          


Ser Asn Lys Gln Asn Phe Ile Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Arg Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Val Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln 
                85                  90                  95      


His Tyr Ser Ser Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Ile 
            100                 105                 110         


Lys Arg Ala Asp Ala Ala Pro Thr Val Ser Ile Phe Pro Pro Ser Ser 
        115                 120                 125             


<210>  15
<211>  450
<212>  PRT
<213>  artificial

<220>
<223>  synthetic

<400>  15

Glu Val Gln Leu Val Glu Ser Gly Gly Asp Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn Asn Tyr 
            20                  25                  30          


Ala Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Gly Ser Ser Gly Ser Gly Gly Asn Thr Tyr His Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Asn Asn Ala Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Lys Gln Gly Asp Trp Asn Trp Gly Ala Phe Asp Ile Trp Gly Gln 
            100                 105                 110         


Gly Thr Met Val Thr Val Ser Ser Ala Lys Thr Thr Ala Pro Ser Val 
        115                 120                 125             


Tyr Pro Leu Ala Pro Val Cys Gly Asp Thr Thr Gly Ser Ser Val Thr 
    130                 135                 140                 


Leu Gly Cys Leu Val Lys Gly Tyr Phe Pro Glu Pro Val Thr Leu Thr 
145                 150                 155                 160 


Trp Asn Ser Gly Ser Leu Ser Ser Gly Val His Thr Phe Pro Ala Val 
                165                 170                 175     


Leu Gln Ser Asp Leu Tyr Thr Leu Ser Ser Ser Val Thr Val Thr Ser 
            180                 185                 190         


Ser Thr Trp Pro Ser Gln Ser Ile Thr Cys Asn Val Ala His Pro Ala 
        195                 200                 205             


Ser Ser Thr Lys Val Asp Lys Lys Ile Glu Pro Arg Gly Pro Thr Ile 
    210                 215                 220                 


Lys Pro Cys Pro Pro Cys Lys Cys Pro Ala Pro Asn Leu Leu Gly Gly 
225                 230                 235                 240 


Pro Ser Val Phe Ile Phe Pro Pro Lys Ile Lys Asp Val Leu Met Ile 
                245                 250                 255     


Ser Leu Ser Pro Ile Val Thr Cys Val Val Val Asp Val Ser Glu Asp 
            260                 265                 270         


Asp Pro Asp Val Gln Ile Ser Trp Phe Val Asn Asn Val Glu Val His 
        275                 280                 285             


Thr Ala Gln Thr Gln Thr His Arg Glu Asp Tyr Asn Ser Thr Leu Arg 
    290                 295                 300                 


Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Met Ser Gly Lys 
305                 310                 315                 320 


Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Glu 
                325                 330                 335     


Arg Thr Ile Ser Lys Pro Lys Gly Ser Val Arg Ala Pro Gln Val Tyr 
            340                 345                 350         


Val Leu Pro Pro Pro Glu Glu Glu Met Thr Lys Lys Gln Val Thr Leu 
        355                 360                 365             


Thr Cys Met Val Thr Asp Phe Met Pro Glu Asp Ile Tyr Val Glu Trp 
    370                 375                 380                 


Thr Asn Asn Gly Lys Thr Glu Leu Asn Tyr Lys Asn Thr Glu Pro Val 
385                 390                 395                 400 


Leu Asp Ser Asp Gly Ser Tyr Phe Met Tyr Ser Lys Leu Arg Val Glu 
                405                 410                 415     


Lys Lys Asn Trp Val Glu Arg Asn Ser Tyr Ser Cys Ser Val Val His 
            420                 425                 430         


Glu Gly Leu His Asn His His Thr Thr Lys Ser Phe Ser Arg Thr Pro 
        435                 440                 445             


Gly Lys 
    450 


<210>  16
<211>  219
<212>  PRT
<213>  artificial

<220>
<223>  synthetic

<400>  16

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Arg Ser Ser Gln Ser Val Phe Tyr Ser 
            20                  25                  30          


Ser Asn Lys Gln Asn Phe Ile Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Arg Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Val Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln 
                85                  90                  95      


His Tyr Ser Ser Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Ile 
            100                 105                 110         


Lys Arg Ala Asp Ala Ala Pro Thr Val Ser Ile Phe Pro Pro Ser Ser 
        115                 120                 125             


Glu Gln Leu Thr Ser Gly Gly Ala Ser Val Val Cys Phe Leu Asn Asn 
    130                 135                 140                 


Phe Tyr Pro Lys Asp Ile Asn Val Lys Trp Lys Ile Asp Gly Ser Glu 
145                 150                 155                 160 


Arg Gln Asn Gly Val Leu Asn Ser Trp Thr Asp Gln Asp Ser Lys Asp 
                165                 170                 175     


Ser Thr Tyr Ser Met Ser Ser Thr Leu Thr Leu Thr Lys Asp Glu Tyr 
            180                 185                 190         


Glu Arg His Asn Ser Tyr Thr Cys Glu Ala Thr His Lys Thr Ser Thr 
        195                 200                 205             


Ser Pro Ile Val Lys Ser Phe Asn Arg Gly Glu 
    210                 215                 


<210>  17
<211>  128
<212>  PRT
<213>  artificial

<220>
<223>  synthetic

<400>  17

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Ile His Trp Glu Lys Gln Arg Pro Glu Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Asn Thr Gly His Thr Glu Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Lys Ala Thr Leu Thr Ala Asp Arg Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Thr Tyr Ser Gly Ser Ser His Phe Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Thr Leu Ile Val Ser Ser Ala Lys Thr Thr Pro Pro Ser Val Tyr 
        115                 120                 125             


<210>  18
<211>  128
<212>  PRT
<213>  artificial

<220>
<223>  synthetic

<400>  18

Asp Ile Val Met Thr Gln Thr Pro Val Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Asn 
            20                  25                  30          


Asn Gly Asp Thr Phe Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Thr 
                85                  90                  95      


Thr Leu Ile Pro Arg Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


Arg Ala Asp Ala Ala Pro Thr Val Ser Ile Phe Pro Pro Ser Ser Glu 
        115                 120                 125             


<210>  19
<211>  443
<212>  PRT
<213>  artificial

<220>
<223>  synthetic

<400>  19

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Ile His Trp Glu Lys Gln Arg Pro Glu Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Asn Thr Gly His Thr Glu Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Lys Ala Thr Leu Thr Ala Asp Arg Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Thr Tyr Ser Gly Ser Ser His Phe Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Thr Leu Ile Val Ser Ser Ala Lys Thr Thr Pro Pro Ser Val Tyr 
        115                 120                 125             


Pro Leu Ala Pro Gly Ser Ala Ala Gln Thr Asn Ser Met Val Thr Leu 
    130                 135                 140                 


Gly Cys Leu Val Lys Gly Tyr Phe Pro Glu Pro Val Thr Val Thr Trp 
145                 150                 155                 160 


Asn Ser Gly Ser Leu Ser Ser Gly Val His Thr Phe Pro Ala Val Leu 
                165                 170                 175     


Gln Ser Asp Leu Tyr Thr Leu Ser Ser Ser Val Thr Val Pro Ser Ser 
            180                 185                 190         


Thr Trp Pro Ser Glu Thr Val Thr Cys Asn Val Ala His Pro Ala Ser 
        195                 200                 205             


Ser Thr Lys Val Asp Lys Lys Ile Val Pro Arg Asp Cys Gly Cys Lys 
    210                 215                 220                 


Pro Cys Ile Cys Thr Val Pro Glu Val Ser Ser Val Phe Ile Phe Pro 
225                 230                 235                 240 


Pro Lys Pro Lys Asp Val Leu Thr Ile Thr Leu Thr Pro Lys Val Thr 
                245                 250                 255     


Cys Val Val Val Asp Ile Ser Lys Asp Asp Pro Glu Val Gln Phe Ser 
            260                 265                 270         


Trp Phe Val Asp Asp Val Glu Val His Thr Ala Gln Thr Gln Pro Arg 
        275                 280                 285             


Glu Glu Gln Phe Asn Ser Thr Phe Arg Ser Val Ser Glu Leu Pro Ile 
    290                 295                 300                 


Met His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys Cys Arg Val Asn 
305                 310                 315                 320 


Ser Ala Ala Phe Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys 
                325                 330                 335     


Gly Arg Pro Lys Ala Pro Gln Val Tyr Thr Ile Pro Pro Pro Lys Glu 
            340                 345                 350         


Gln Met Ala Lys Asp Lys Val Ser Leu Thr Cys Met Ile Thr Asp Phe 
        355                 360                 365             


Phe Pro Glu Asp Ile Thr Val Glu Trp Gln Trp Asn Gly Gln Pro Ala 
    370                 375                 380                 


Glu Asn Tyr Lys Asn Thr Gln Pro Ile Met Asp Thr Asp Gly Ser Tyr 
385                 390                 395                 400 


Phe Val Tyr Ser Lys Leu Asn Val Gln Lys Ser Asn Trp Glu Ala Gly 
                405                 410                 415     


Asn Thr Phe Thr Cys Ser Val Leu His Glu Gly Leu His Asn His His 
            420                 425                 430         


Thr Glu Lys Ser Leu Ser His Ser Pro Gly Lys 
        435                 440             


<210>  20
<211>  219
<212>  PRT
<213>  artificial

<220>
<223>  synthetic

<400>  20

Asp Ile Val Met Thr Gln Thr Pro Val Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Asn 
            20                  25                  30          


Asn Gly Asp Thr Phe Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Thr 
                85                  90                  95      


Thr Leu Ile Pro Arg Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


Arg Ala Asp Ala Ala Pro Thr Val Ser Ile Phe Pro Pro Ser Ser Glu 
        115                 120                 125             


Gln Leu Thr Ser Gly Gly Ala Ser Val Val Cys Phe Leu Asn Asn Phe 
    130                 135                 140                 


Tyr Pro Lys Asp Ile Asn Val Lys Trp Lys Ile Asp Gly Ser Glu Arg 
145                 150                 155                 160 


Gln Asn Gly Val Leu Asn Ser Trp Thr Asp Gln Asp Ser Lys Asp Ser 
                165                 170                 175     


Thr Tyr Ser Met Ser Ser Thr Leu Thr Leu Thr Lys Asp Glu Tyr Glu 
            180                 185                 190         


Arg His Asn Ser Tyr Thr Cys Glu Ala Thr His Lys Thr Ser Thr Ser 
        195                 200                 205             


Pro Ile Val Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215                 


