
                                SEQUENCE LISTING

<110> Trelys, Inc.
      Bradshaw, Jill

<120> COMPOSITIONS AND METHODS FOR BIOLOGICAL
  PRODUCTION OF METHIONINE
  

<130> 910215.407WO

<140> PCT                 
<141> 2016-05-06  

<150> US 62/157,797       
<151> 2015-05-06  

<160> 32

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 1542
<212> DNA
<213> Archaea sp.

<400> 1
ttgaaaaccg ttcacgaaat taacgagaag atacgaaatg gcgatgcagt tgtagtcact      60
gcagaagaga tgatcgatat agtcgacgaa ctgggtgcag aaaaagcggc agttgaaata     120
gatgtagtta cgacaggtac atttggagca atgtgttcaa gcggagcatt tttaaatttt     180
ggacactctg atccgccaat aaaaatgtgt aaaacatatt tgaatggtgt ggaagcatat     240
tcaggaattg cagcagttga tgcttattta ggggctactc aaacgaatag tgatgatgat     300
attgatatat catatggggg ttcccacgtt cttgaagatc ttgtagctgg aaaagaaatt     360
gaacttgtag cggaaggata cactactgac tgctacccta gaaaaaaagt agaaacaacg     420
ataacaattg atgatttaaa tcaagcaatc ttagtaaatc caagaaactg ctaccaatct     480
tacaatggtg ctacgaacag tactgaagaa aaaatataca cttacatggg tgcacttctc     540
ccagaatttg gaaatttaaa ttattccggt gcaggtcaat taaatccact gcaaaacgat     600
ttcaataaag aaacaaaaac ttataacacc ttaggaatgg gtacaagaat cttcttaggc     660
ggtgcacaag gttatattgc aggttcagga acccagcaca gtccaaatgg tggatttgga     720
accttgatgg ttcaagggga cctaaaagaa atgagtacta aatatttaag aggagcaacg     780
attccaaaat acggaagtac gctttacatg gggatcggaa ttccaattcc agtattaaac     840
gcagaaattg caaaaacctg tgcaataaaa gatgaagata ttgcaatacc catattggat     900
tacggaattc caagaaggga taaacctgaa ttaggcgtta caaactataa agatgcaaga     960
tctggaaaag taacgattga agttgaaatc gaaggcaaaa aagtagataa atgcatgaga    1020
tctgcatcag tttcaagtta taaagtttca agggaaattt caaaagaact taaaaactgg    1080
atttcaaata gtgaatttat gttaacacaa agacttgaac ctttaaaaag tgcagctcca    1140
aaaccaatga aagcaaaaat gaaacttgta aaagatatat tgagcaggcc tgtagttgta    1200
ggaagcttaa atacctcaat tacacaagct tcaagagttt taattgaaaa taatataaac    1260
catctgccaa ttgtggatga aaacggcaaa ctttcaggaa taatcacttc atgggatatt    1320
gcaaaggcaa tggcgcagga taaacattca atttctgaaa tcatgactac atatatagta    1380
tctgcaacac cagatgaaac tatagatatg gcagcaagaa aaatgagcag aaacaatatt    1440
tcaggacttc ctgttgtcga ttcaaacaac aaggttcttg gggtggtttc agctgaagat    1500
atctcaaaac ttatcgggag aaatggactt cataaaattt aa                       1542

<210> 2
<211> 1542
<212> DNA
<213> Artificial Sequence

<220> 
<223> Archaea mutant sequence

<400> 2
ttgaaaaccg ttcacgaaat taacgagaag atacgaaatg gcgatgcagt tgtagtcact      60
gcagaagaga tgatcgatat agtcgacgaa ctgggtgcag aaaaagcggc agttgaaata     120
gatgtagtta cgacaggtac atttggagca atgtgttcaa gcggagcatt tttaaatttt     180
ggacactctg atccgccaat aaaaatgtgt aaaacatatt tgaatggtgt ggaagcatat     240
tcaggaattg cagcagttga tgcttattta ggggctactc aaacgaatag tgatgatgat     300
attgatatat catatggggg ttcccacgtt cttgaagatc ttgtagctgg aaaagaaatt     360
gaacttgtag cggaaggata cactactgac tgctacccta gaaaaaaagt agaaacaacg     420
ataacaattg atgatttaaa tcaagcaatc ttagtaaatc caagaaactg ctaccaatct     480
tacaatggtg ctacgaacag tactgaagaa aaaatataca cttacatggg tgcacttctc     540
ccagaatttg gaaatttaaa ttattccggt gcaggtcaat taaatccact gcaaaacgat     600
ttcaataaag aaacaaaaac ttataacacc ttaggaatgg gtacaagaat cttcttaggc     660
ggtgcacaag gttatattgc aggttcagga acccagcaca gtccaaatgg tggatttgga     720
accttgatgg ttcaagggga cctaaaagaa atgagtacta aatatttaag aggagcaacg     780
attccaaaat acggaagtac gctttacatg gggatcggaa ttccaattcc agtattaaac     840
gcagaaattg caaaaacctg tgcaataaaa gatgaagata ttgcaatacc catattggat     900
tacggaattc caagaaggga taaacctgaa ttaggcgtta caaactataa agatgcaaga     960
tctggaaaag taacgattga agttgaaatc gaaggcaaaa aagtagataa atgcatgaga    1020
tctgcatcag tttcaagtta taaagtttca agggaaattt caaaagaact taaaaactgg    1080
atttcaaata gtgaatttat gttaacacaa agacttgaac ctttaaaaag tgcagctcca    1140
aaaccaatga aagcaaaaat gaaacttgta aaagatatat tgagcaggcc tgtagttgta    1200
ggaagcttaa atacctcaat tacacaagct tcaagagttt taattgaaaa taatataaac    1260
catctgccaa ttgtggatga aaacggcaaa ctttcaggaa taatcacttc atggaatatt    1320
gcaaaggcaa tggcgcagga taaacattca atttctgaaa tcatgactac atatatagta    1380
tctgcaacac cagatgaaac tatagatatg gcagcaagaa aaatgagcag aaacaatatt    1440
tcaggacttc ctgttgtcga ttcaaacaac aaggttcttg gggtggtttc agctgaagat    1500
atctcaaaac ttatcgggag aaatggactt cataaaattt aa                       1542

<210> 3
<211> 513
<212> PRT
<213> Archaea sp.

<400> 3
Met Lys Thr Val His Glu Ile Asn Glu Lys Ile Arg Asn Gly Asp Ala
 1               5                  10                  15
Val Val Val Thr Ala Glu Glu Met Ile Asp Ile Val Asp Glu Leu Gly
            20                  25                  30
Ala Glu Lys Ala Ala Val Glu Ile Asp Val Val Thr Thr Gly Thr Phe
        35                  40                  45
Gly Ala Met Cys Ser Ser Gly Ala Phe Leu Asn Phe Gly His Ser Asp
    50                  55                  60
Pro Pro Ile Lys Met Cys Lys Thr Tyr Leu Asn Gly Val Glu Ala Tyr
65                  70                  75                  80
Ser Gly Ile Ala Ala Val Asp Ala Tyr Leu Gly Ala Thr Gln Thr Asn
                85                  90                  95
Ser Asp Asp Asp Ile Asp Ile Ser Tyr Gly Gly Ser His Val Leu Glu
            100                 105                 110
Asp Leu Val Ala Gly Lys Glu Ile Glu Leu Val Ala Glu Gly Tyr Thr
        115                 120                 125
Thr Asp Cys Tyr Pro Arg Lys Lys Val Glu Thr Thr Ile Thr Ile Asp
    130                 135                 140
Asp Leu Asn Gln Ala Ile Leu Val Asn Pro Arg Asn Cys Tyr Gln Ser
145                 150                 155                 160
Tyr Asn Gly Ala Thr Asn Ser Thr Glu Glu Lys Ile Tyr Thr Tyr Met
                165                 170                 175
Gly Ala Leu Leu Pro Glu Phe Gly Asn Leu Asn Tyr Ser Gly Ala Gly
            180                 185                 190
Gln Leu Asn Pro Leu Gln Asn Asp Phe Asn Lys Glu Thr Lys Thr Tyr
        195                 200                 205
Asn Thr Leu Gly Met Gly Thr Arg Ile Phe Leu Gly Gly Ala Gln Gly
    210                 215                 220
Tyr Ile Ala Gly Ser Gly Thr Gln His Ser Pro Asn Gly Gly Phe Gly
225                 230                 235                 240
Thr Leu Met Val Gln Gly Asp Leu Lys Glu Met Ser Thr Lys Tyr Leu
                245                 250                 255
Arg Gly Ala Thr Ile Pro Lys Tyr Gly Ser Thr Leu Tyr Met Gly Ile
            260                 265                 270
Gly Ile Pro Ile Pro Val Leu Asn Ala Glu Ile Ala Lys Thr Cys Ala
        275                 280                 285
Ile Lys Asp Glu Asp Ile Ala Ile Pro Ile Leu Asp Tyr Gly Ile Pro
    290                 295                 300
Arg Arg Asp Lys Pro Glu Leu Gly Val Thr Asn Tyr Lys Asp Ala Arg
305                 310                 315                 320
Ser Gly Lys Val Thr Ile Glu Val Glu Ile Glu Gly Lys Lys Val Asp
                325                 330                 335
Lys Cys Met Arg Ser Ala Ser Val Ser Ser Tyr Lys Val Ser Arg Glu
            340                 345                 350
Ile Ser Lys Glu Leu Lys Asn Trp Ile Ser Asn Ser Glu Phe Met Leu
        355                 360                 365
Thr Gln Arg Leu Glu Pro Leu Lys Ser Ala Ala Pro Lys Pro Met Lys
    370                 375                 380
Ala Lys Met Lys Leu Val Lys Asp Ile Leu Ser Arg Pro Val Val Val
385                 390                 395                 400
Gly Ser Leu Asn Thr Ser Ile Thr Gln Ala Ser Arg Val Leu Ile Glu
                405                 410                 415
Asn Asn Ile Asn His Leu Pro Ile Val Asp Glu Asn Gly Lys Leu Ser
            420                 425                 430
Gly Ile Ile Thr Ser Trp Asp Ile Ala Lys Ala Met Ala Gln Asp Lys
        435                 440                 445
His Ser Ile Ser Glu Ile Met Thr Thr Tyr Ile Val Ser Ala Thr Pro
    450                 455                 460
Asp Glu Thr Ile Asp Met Ala Ala Arg Lys Met Ser Arg Asn Asn Ile
465                 470                 475                 480
Ser Gly Leu Pro Val Val Asp Ser Asn Asn Lys Val Leu Gly Val Val
                485                 490                 495
Ser Ala Glu Asp Ile Ser Lys Leu Ile Gly Arg Asn Gly Leu His Lys
            500                 505                 510
Ile


<210> 4
<211> 513
<212> PRT
<213> Artificial Sequence

<220> 
<223> Archaea mutant sequence

<400> 4
Met Lys Thr Val His Glu Ile Asn Glu Lys Ile Arg Asn Gly Asp Ala
 1               5                  10                  15
Val Val Val Thr Ala Glu Glu Met Ile Asp Ile Val Asp Glu Leu Gly
            20                  25                  30
Ala Glu Lys Ala Ala Val Glu Ile Asp Val Val Thr Thr Gly Thr Phe
        35                  40                  45
Gly Ala Met Cys Ser Ser Gly Ala Phe Leu Asn Phe Gly His Ser Asp
    50                  55                  60
Pro Pro Ile Lys Met Cys Lys Thr Tyr Leu Asn Gly Val Glu Ala Tyr
65                  70                  75                  80
Ser Gly Ile Ala Ala Val Asp Ala Tyr Leu Gly Ala Thr Gln Thr Asn
                85                  90                  95
Ser Asp Asp Asp Ile Asp Ile Ser Tyr Gly Gly Ser His Val Leu Glu
            100                 105                 110
Asp Leu Val Ala Gly Lys Glu Ile Glu Leu Val Ala Glu Gly Tyr Thr
        115                 120                 125
Thr Asp Cys Tyr Pro Arg Lys Lys Val Glu Thr Thr Ile Thr Ile Asp
    130                 135                 140
Asp Leu Asn Gln Ala Ile Leu Val Asn Pro Arg Asn Cys Tyr Gln Ser
145                 150                 155                 160
Tyr Asn Gly Ala Thr Asn Ser Thr Glu Glu Lys Ile Tyr Thr Tyr Met
                165                 170                 175
Gly Ala Leu Leu Pro Glu Phe Gly Asn Leu Asn Tyr Ser Gly Ala Gly
            180                 185                 190
Gln Leu Asn Pro Leu Gln Asn Asp Phe Asn Lys Glu Thr Lys Thr Tyr
        195                 200                 205
Asn Thr Leu Gly Met Gly Thr Arg Ile Phe Leu Gly Gly Ala Gln Gly
    210                 215                 220
Tyr Ile Ala Gly Ser Gly Thr Gln His Ser Pro Asn Gly Gly Phe Gly
225                 230                 235                 240
Thr Leu Met Val Gln Gly Asp Leu Lys Glu Met Ser Thr Lys Tyr Leu
                245                 250                 255
Arg Gly Ala Thr Ile Pro Lys Tyr Gly Ser Thr Leu Tyr Met Gly Ile
            260                 265                 270
Gly Ile Pro Ile Pro Val Leu Asn Ala Glu Ile Ala Lys Thr Cys Ala
        275                 280                 285
Ile Lys Asp Glu Asp Ile Ala Ile Pro Ile Leu Asp Tyr Gly Ile Pro
    290                 295                 300
Arg Arg Asp Lys Pro Glu Leu Gly Val Thr Asn Tyr Lys Asp Ala Arg
305                 310                 315                 320
Ser Gly Lys Val Thr Ile Glu Val Glu Ile Glu Gly Lys Lys Val Asp
                325                 330                 335
Lys Cys Met Arg Ser Ala Ser Val Ser Ser Tyr Lys Val Ser Arg Glu
            340                 345                 350
Ile Ser Lys Glu Leu Lys Asn Trp Ile Ser Asn Ser Glu Phe Met Leu
        355                 360                 365
Thr Gln Arg Leu Glu Pro Leu Lys Ser Ala Ala Pro Lys Pro Met Lys
    370                 375                 380
Ala Lys Met Lys Leu Val Lys Asp Ile Leu Ser Arg Pro Val Val Val
385                 390                 395                 400
Gly Ser Leu Asn Thr Ser Ile Thr Gln Ala Ser Arg Val Leu Ile Glu
                405                 410                 415
Asn Asn Ile Asn His Leu Pro Ile Val Asp Glu Asn Gly Lys Leu Ser
            420                 425                 430
Gly Ile Ile Thr Ser Trp Asn Ile Ala Lys Ala Met Ala Gln Asp Lys
        435                 440                 445
His Ser Ile Ser Glu Ile Met Thr Thr Tyr Ile Val Ser Ala Thr Pro
    450                 455                 460
Asp Glu Thr Ile Asp Met Ala Ala Arg Lys Met Ser Arg Asn Asn Ile
465                 470                 475                 480
Ser Gly Leu Pro Val Val Asp Ser Asn Asn Lys Val Leu Gly Val Val
                485                 490                 495
Ser Ala Glu Asp Ile Ser Lys Leu Ile Gly Arg Asn Gly Leu His Lys
            500                 505                 510
Ile


<210> 5
<211> 399
<212> DNA
<213> Archaea sp.

<400> 5
gtgaaaaaga gagtatttta ctggatatct ggaagcaacg taagagaacc ggtagtttca      60
aacgtggttt tagaaactgg tgtaatggta aatattttaa aggcaaaaat ggagccaagg     120
gaaggatttt taattctcga attaactggc gatgaagaac agatcgaaaa atcaattgaa     180
atactgaaaa aattcgggga agcagaagat atcccaaaaa tcatccaaaa agatgatgaa     240
aaatgcatcg attgtggtgc atgtgtcgtt cactgccccg ttggtgcgct ttcagttgac     300
gaagaattta aaatactgct cgacgaagac gaatgcattg gatgtaaaaa ctgtgcaaaa     360
atatgccctg taaatgcaat taaaatattt gaaatctaa                            399

<210> 6
<211> 399
<212> DNA
<213> Artificial Sequence

<220> 
<223> Archaea mutant sequence

<400> 6
gtgaaaaaga gagtatttta ctggatatct ggaagcaacg taagagaacc ggtagtttca      60
aacgtggttt tagaaactgg tgtaatggta aatattttaa aggcaaaaat ggagccaagg     120
gaaggatttt taattctcga attaactggc gatgaagaac agatcgaaaa atcaattgaa     180
atactgaaaa aattcgggga agcagaagat atcccaaaaa tcatccaaaa agatgatgaa     240
aaatgcatcg attgtggtgc atgtgtcgtt cactgccccg ttggtgcgct ttcagttgac     300
gaagaattta aaatactgct cgacgaagac gaatgcattg aatgtaaaaa ctgtgcaaaa     360
atatgccctg taaatgcaat taaaatattt gaaatctaa                            399

<210> 7
<211> 132
<212> PRT
<213> Archaea sp.

<400> 7
Met Lys Lys Arg Val Phe Tyr Trp Ile Ser Gly Ser Asn Val Arg Glu
 1               5                  10                  15
Pro Val Val Ser Asn Val Val Leu Glu Thr Gly Val Met Val Asn Ile
            20                  25                  30
Leu Lys Ala Lys Met Glu Pro Arg Glu Gly Phe Leu Ile Leu Glu Leu
        35                  40                  45
Thr Gly Asp Glu Glu Gln Ile Glu Lys Ser Ile Glu Ile Leu Lys Lys
    50                  55                  60
Phe Gly Glu Ala Glu Asp Ile Pro Lys Ile Ile Gln Lys Asp Asp Glu
65                  70                  75                  80
Lys Cys Ile Asp Cys Gly Ala Cys Val Val His Cys Pro Val Gly Ala
                85                  90                  95
Leu Ser Val Asp Glu Glu Phe Lys Ile Leu Leu Asp Glu Asp Glu Cys
            100                 105                 110
Ile Gly Cys Lys Asn Cys Ala Lys Ile Cys Pro Val Asn Ala Ile Lys
        115                 120                 125
Ile Phe Glu Ile
    130

<210> 8
<211> 132
<212> PRT
<213> Artificial Sequence

<220> 
<223> Archaea mutant sequence

<400> 8
Met Lys Lys Arg Val Phe Tyr Trp Ile Ser Gly Ser Asn Val Arg Glu
 1               5                  10                  15
Pro Val Val Ser Asn Val Val Leu Glu Thr Gly Val Met Val Asn Ile
            20                  25                  30
Leu Lys Ala Lys Met Glu Pro Arg Glu Gly Phe Leu Ile Leu Glu Leu
        35                  40                  45
Thr Gly Asp Glu Glu Gln Ile Glu Lys Ser Ile Glu Ile Leu Lys Lys
    50                  55                  60
Phe Gly Glu Ala Glu Asp Ile Pro Lys Ile Ile Gln Lys Asp Asp Glu
65                  70                  75                  80
Lys Cys Ile Asp Cys Gly Ala Cys Val Val His Cys Pro Val Gly Ala
                85                  90                  95
Leu Ser Val Asp Glu Glu Phe Lys Ile Leu Leu Asp Glu Asp Glu Cys
            100                 105                 110
Ile Glu Cys Lys Asn Cys Ala Lys Ile Cys Pro Val Asn Ala Ile Lys
        115                 120                 125
Ile Phe Glu Ile
    130

<210> 9
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer sequence

<400> 9
ctatagaact aacccaatg                                                   19

<210> 10
<211> 17
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer sequence

<400> 10
ggtgttgcag atactat                                                     17

<210> 11
<211> 15
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer sequence

<400> 11
agacttgaac cttta                                                       15

<210> 12
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer sequence

<400> 12
cgccaaaatc ttccctgc                                                    18

<210> 13
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> Forward Primer sequence

<400> 13
gggacggcgc aacaaatgg                                                   19

<210> 14
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> Reverse Primer sequence

<400> 14
ggagatagtg agacccctgg agt                                              23

<210> 15
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer sequence

<400> 15
aatagttacc ggcgttacaa tca                                              23

<210> 16
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer sequence

<400> 16
tccctgatcg atagaaagtg tagt                                             24

<210> 17
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer sequence

<400> 17
ttgccgatga aattaaagtg aaa                                              23

<210> 18
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer sequence

<400> 18
gcgggcgcgc cgcataatta caccttatgc gttc                                  34

<210> 19
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer sequence

<400> 19
gcgggcgcgc ctaatcacgg ttcgtgatac tat                                   33

<210> 20
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer sequence

<400> 20
attacgccaa gcttggtacc actctcttct tcttcaggga                            40

<210> 21
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer sequence

<400> 21
gtggatccga gctcggtacc tgagatcccc gcgctggagg                            40

<210> 22
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer sequence

<400> 22
gtattgaatt ggcaaact                                                    18

<210> 23
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer sequence

<400> 23
accggctcag acccggtg                                                    18

<210> 24
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer sequence

<400> 24
ggaaagaact cgacgtgc                                                    18

<210> 25
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer sequence

<400> 25
actgacattc ttgattatg                                                   19

<210> 26
<211> 17
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer sequence

<400> 26
cttgcagcgc gcaggct                                                     17

<210> 27
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer sequence

<400> 27
aaattatgag gcgcgcctcc ctgaagaaga agagag                                36

<210> 28
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer sequence

<400> 28
tgcttattcg gcgcgccagt tccattttac cacc                                  34

<210> 29
<211> 1503
<212> DNA
<213> Archaea sp.

<400> 29
atggttgaaa aatcggttca tgagatcaat aaaaaaattg aagatggaag cgtcaatgta      60
gtcacagccg aggaaatggt cggaattgtt gaaaaccttg gcgtggaagg tgctgcaaga     120
gaagttgatg tggtcactac aggcacgttc ggagccatgt gctcttcagg tttgatgctt     180
aatctcggac attccgaacc cccgatcaag atccagaaac tctggtttaa caacgtggag     240
gcatacagcg ggcttgctgc tgtggatgct tatctggggg ctgcccagat atcggataca     300
agaggaatac agtatggtgg agcgcacgtt attgaagacc tgctgagagg gaaagaactc     360
gacgtgcatg caacttccta tgggacagac tgctatccca ggaaagtgct tgatacgaga     420
attaccctcg atgacttaaa cgaggcagtc cttctcaacc ccagaaacgc ttaccagaaa     480
tatgccgctg caacaaacag ttcaaaaagg attctgaaca cctatatggg agagcttcta     540
cccaatttcg gaaacgtaac ttattccggg gcaggagtgc tttctcccct ttcaaatgat     600
cctgactacg aaactatcgg gatgggcaca aggattttca tgggaggagc ccagggctat     660
attataggca atgggaccca gcattctccc tcaagcagtt ttgggaccct tatgcttaaa     720
ggaaacctga aagaaatgag ctccgattat ttaagggctg cttcttttgc aggctacgga     780
acaactcttt acatgggaat cggaatcccc atacccattc tgaatgaaaa aatcgcagcc     840
tcaactgcgg tgcgtgatga agacattttt actgacattc ttgattatgc cgtgggcagc     900
agggataagc ctgtgataaa gcaggtaaac tatgccgagc tcaggtcagg ctcgatagag     960
cttgaaggga agaacacacc gacctcatcc ctctcaagtt tcaagaacgc cagaaagatt    1020
gcaaatgagc taaaggaatg ggttaagcac ggaaaattct ttgtcagcat gcccgtagaa    1080
aagctttccc gcgagggctc ggcaaagtcc atgaaacaga ctcaggcagt cccactcgta    1140
aaagacgtca tggcagactt tattgttacg atcaaaaaga accagacggt tcaggacgct    1200
gcaaagaaga tctgggaaaa ctcttttaac caccttgctg tggtttcgga tacaggggaa    1260
ctggtaggaa tcctgacggc ctgggatatc tcaaaagccg ttgccgaaaa tatatttgat    1320
tccgtagaaa gtgtcatgac gaaaaaagtc cttacctgcg ccccgaacga acccgtggac    1380
cttgcagcgc gcaggcttga ccgctatggc gtttcggcaa tgcctgtaat cgatacacag    1440
agaaaagtac tcggaataat tacgagcgac aatataagca agcttctcgc aaggaggtac    1500
tga                                                                  1503

<210> 30
<211> 500
<212> PRT
<213> Archaea sp.

<400> 30
Met Val Glu Lys Ser Val His Glu Ile Asn Lys Lys Ile Glu Asp Gly
 1               5                  10                  15
Ser Val Asn Val Val Thr Ala Glu Glu Met Val Gly Ile Val Glu Asn
            20                  25                  30
Leu Gly Val Glu Gly Ala Ala Arg Glu Val Asp Val Val Thr Thr Gly
        35                  40                  45
Thr Phe Gly Ala Met Cys Ser Ser Gly Leu Met Leu Asn Leu Gly His
    50                  55                  60
Ser Glu Pro Pro Ile Lys Ile Gln Lys Leu Trp Phe Asn Asn Val Glu
65                  70                  75                  80
Ala Tyr Ser Gly Leu Ala Ala Val Asp Ala Tyr Leu Gly Ala Ala Gln
                85                  90                  95
Ile Ser Asp Thr Arg Gly Ile Gln Tyr Gly Gly Ala His Val Ile Glu
            100                 105                 110
Asp Leu Leu Arg Gly Lys Glu Leu Asp Val His Ala Thr Ser Tyr Gly
        115                 120                 125
Thr Asp Cys Tyr Pro Arg Lys Val Leu Asp Thr Arg Ile Thr Leu Asp
    130                 135                 140
Asp Leu Asn Glu Ala Val Leu Leu Asn Pro Arg Asn Ala Tyr Gln Lys
145                 150                 155                 160
Tyr Ala Ala Ala Thr Asn Ser Ser Lys Arg Ile Leu Asn Thr Tyr Met
                165                 170                 175
Gly Glu Leu Leu Pro Asn Phe Gly Asn Val Thr Tyr Ser Gly Ala Gly
            180                 185                 190
Val Leu Ser Pro Leu Ser Asn Asp Pro Asp Tyr Glu Thr Ile Gly Met
        195                 200                 205
Gly Thr Arg Ile Phe Met Gly Gly Ala Gln Gly Tyr Ile Ile Gly Asn
    210                 215                 220
Gly Thr Gln His Ser Pro Ser Ser Ser Phe Gly Thr Leu Met Leu Lys
225                 230                 235                 240
Gly Asn Leu Lys Glu Met Ser Ser Asp Tyr Leu Arg Ala Ala Ser Phe
                245                 250                 255
Ala Gly Tyr Gly Thr Thr Leu Tyr Met Gly Ile Gly Ile Pro Ile Pro
            260                 265                 270
Ile Leu Asn Glu Lys Ile Ala Ala Ser Thr Ala Val Arg Asp Glu Asp
        275                 280                 285
Ile Phe Thr Asp Ile Leu Asp Tyr Ala Val Gly Ser Arg Asp Lys Pro
    290                 295                 300
Val Ile Lys Gln Val Asn Tyr Ala Glu Leu Arg Ser Gly Ser Ile Glu
305                 310                 315                 320
Leu Glu Gly Lys Asn Thr Pro Thr Ser Ser Leu Ser Ser Phe Lys Asn
                325                 330                 335
Ala Arg Lys Ile Ala Asn Glu Leu Lys Glu Trp Val Lys His Gly Lys
            340                 345                 350
Phe Phe Val Ser Met Pro Val Glu Lys Leu Ser Arg Glu Gly Ser Ala
        355                 360                 365
Lys Ser Met Lys Gln Thr Gln Ala Val Pro Leu Val Lys Asp Val Met
    370                 375                 380
Ala Asp Phe Ile Val Thr Ile Lys Lys Asn Gln Thr Val Gln Asp Ala
385                 390                 395                 400
Ala Lys Lys Ile Trp Glu Asn Ser Phe Asn His Leu Ala Val Val Ser
                405                 410                 415
Asp Thr Gly Glu Leu Val Gly Ile Leu Thr Ala Trp Asp Ile Ser Lys
            420                 425                 430
Ala Val Ala Glu Asn Ile Phe Asp Ser Val Glu Ser Val Met Thr Lys
        435                 440                 445
Lys Val Leu Thr Cys Ala Pro Asn Glu Pro Val Asp Leu Ala Ala Arg
    450                 455                 460
Arg Leu Asp Arg Tyr Gly Val Ser Ala Met Pro Val Ile Asp Thr Gln
465                 470                 475                 480
Arg Lys Val Leu Gly Ile Ile Thr Ser Asp Asn Ile Ser Lys Leu Leu
                485                 490                 495
Ala Arg Arg Tyr
            500

<210> 31
<211> 1503
<212> DNA
<213> Artificial Sequence

<220> 
<223> Archaea mutant sequence

<400> 31
atggttgaaa aatcggttca tgagatcaat aaaaaaattg aagatggaag cgtcaatgta      60
gtcacagccg aggaaatggt cggaattgtt gaaaaccttg gcgtggaagg tgctgcaaga     120
gaagttgatg tggtcactac aggcacgttc ggagccatgt gctcttcagg tttgatgctt     180
aatctcggac attccgaacc cccgatcaag atccagaaac tctggtttaa caacgtggag     240
gcatacagcg ggcttgctgc tgtggatgct tatctggggg ctgcccagat atcggataca     300
agaggaatac agtatggtgg agcgcacgtt attgaagacc tgctgagagg gaaagaactc     360
gacgtgcatg caacttccta tgggacagac tgctatccca ggaaagtgct tgatacgaga     420
attaccctcg atgacttaaa cgaggcagtc cttctcaacc ccagaaacgc ttaccagaaa     480
tatgccgctg caacaaacag ttcaaaaagg attctgaaca cctatatggg agagcttcta     540
cccaatttcg gaaacgtaac ttattccggg gcaggagtgc tttctcccct ttcaaatgat     600
cctgactacg aaactatcgg gatgggcaca aggattttca tgggaggagc ccagggctat     660
attataggca atgggaccca gcattctccc tcaagcagtt ttgggaccct tatgcttaaa     720
ggaaacctga aagaaatgag ctccgattat ttaagggctg cttcttttgc aggctacgga     780
acaactcttt acatgggaat cggaatcccc atacccattc tgaatgaaaa aatcgcagcc     840
tcaactgcgg tgcgtgatga agacattttt actgacattc ttgattatgc cgtgggcagc     900
agggataagc ctgtgataaa gcaggtaaac tatgccgagc tcaggtcagg ctcgatagag     960
cttgaaggga agaacacacc gacctcatcc ctctcaagtt tcaagaacgc cagaaagatt    1020
gcaaatgagc taaaggaatg ggttaagcac ggaaaattct ttgtcagcat gcccgtagaa    1080
aagctttccc gcgagggctc ggcaaagtcc atgaaacaga ctcaggcagt cccactcgta    1140
aaagacgtca tggcagactt tattgttacg atcaaaaaga accagacggt tcaggacgct    1200
gcaaagaaga tctgggaaaa ctcttttaac caccttgctg tggtttcgga tacaggggaa    1260
ctggtaggaa tcctgacggc ctgggatatc tcaaaagccg ttgccgaaaa tatatttgat    1320
tccgtagaaa gtgtcatgac gaaaaaagtc cttacctgcg ccccgaacga acccgtggac    1380
cttgcagcgc gcaggcttga ccgctatggc gtttcggcaa tgcctgtaat cgatacacag    1440
agaaaagtac tcggaataat tacgaacgac aatataagca agcttctcgc aaggaggtac    1500
tga                                                                  1503

<210> 32
<211> 500
<212> PRT
<213> Artificial Sequence

<220> 
<223> Archaea mutant sequence

<400> 32
Met Val Glu Lys Ser Val His Glu Ile Asn Lys Lys Ile Glu Asp Gly
 1               5                  10                  15
Ser Val Asn Val Val Thr Ala Glu Glu Met Val Gly Ile Val Glu Asn
            20                  25                  30
Leu Gly Val Glu Gly Ala Ala Arg Glu Val Asp Val Val Thr Thr Gly
        35                  40                  45
Thr Phe Gly Ala Met Cys Ser Ser Gly Leu Met Leu Asn Leu Gly His
    50                  55                  60
Ser Glu Pro Pro Ile Lys Ile Gln Lys Leu Trp Phe Asn Asn Val Glu
65                  70                  75                  80
Ala Tyr Ser Gly Leu Ala Ala Val Asp Ala Tyr Leu Gly Ala Ala Gln
                85                  90                  95
Ile Ser Asp Thr Arg Gly Ile Gln Tyr Gly Gly Ala His Val Ile Glu
            100                 105                 110
Asp Leu Leu Arg Gly Lys Glu Leu Asp Val His Ala Thr Ser Tyr Gly
        115                 120                 125
Thr Asp Cys Tyr Pro Arg Lys Val Leu Asp Thr Arg Ile Thr Leu Asp
    130                 135                 140
Asp Leu Asn Glu Ala Val Leu Leu Asn Pro Arg Asn Ala Tyr Gln Lys
145                 150                 155                 160
Tyr Ala Ala Ala Thr Asn Ser Ser Lys Arg Ile Leu Asn Thr Tyr Met
                165                 170                 175
Gly Glu Leu Leu Pro Asn Phe Gly Asn Val Thr Tyr Ser Gly Ala Gly
            180                 185                 190
Val Leu Ser Pro Leu Ser Asn Asp Pro Asp Tyr Glu Thr Ile Gly Met
        195                 200                 205
Gly Thr Arg Ile Phe Met Gly Gly Ala Gln Gly Tyr Ile Ile Gly Asn
    210                 215                 220
Gly Thr Gln His Ser Pro Ser Ser Ser Phe Gly Thr Leu Met Leu Lys
225                 230                 235                 240
Gly Asn Leu Lys Glu Met Ser Ser Asp Tyr Leu Arg Ala Ala Ser Phe
                245                 250                 255
Ala Gly Tyr Gly Thr Thr Leu Tyr Met Gly Ile Gly Ile Pro Ile Pro
            260                 265                 270
Ile Leu Asn Glu Lys Ile Ala Ala Ser Thr Ala Val Arg Asp Glu Asp
        275                 280                 285
Ile Phe Thr Asp Ile Leu Asp Tyr Ala Val Gly Ser Arg Asp Lys Pro
    290                 295                 300
Val Ile Lys Gln Val Asn Tyr Ala Glu Leu Arg Ser Gly Ser Ile Glu
305                 310                 315                 320
Leu Glu Gly Lys Asn Thr Pro Thr Ser Ser Leu Ser Ser Phe Lys Asn
                325                 330                 335
Ala Arg Lys Ile Ala Asn Glu Leu Lys Glu Trp Val Lys His Gly Lys
            340                 345                 350
Phe Phe Val Ser Met Pro Val Glu Lys Leu Ser Arg Glu Gly Ser Ala
        355                 360                 365
Lys Ser Met Lys Gln Thr Gln Ala Val Pro Leu Val Lys Asp Val Met
    370                 375                 380
Ala Asp Phe Ile Val Thr Ile Lys Lys Asn Gln Thr Val Gln Asp Ala
385                 390                 395                 400
Ala Lys Lys Ile Trp Glu Asn Ser Phe Asn His Leu Ala Val Val Ser
                405                 410                 415
Asp Thr Gly Glu Leu Val Gly Ile Leu Thr Ala Trp Asp Ile Ser Lys
            420                 425                 430
Ala Val Ala Glu Asn Ile Phe Asp Ser Val Glu Ser Val Met Thr Lys
        435                 440                 445
Lys Val Leu Thr Cys Ala Pro Asn Glu Pro Val Asp Leu Ala Ala Arg
    450                 455                 460
Arg Leu Asp Arg Tyr Gly Val Ser Ala Met Pro Val Ile Asp Thr Gln
465                 470                 475                 480
Arg Lys Val Leu Gly Ile Ile Thr Asn Asp Asn Ile Ser Lys Leu Leu
                485                 490                 495
Ala Arg Arg Tyr
            500
