                         SEQUENCE LISTING

<110>  Dewey, Ralph E.
       Lewis, Ramsey S.
 
<120>  METHODS AND COMPOSITIONS FOR REDUCING THE TOBACCO SPECIFIC 
       NITROSAMINE NNK IN TOBACCO

<130>  5051-880WO

<150>  US 62/156,981
<151>  2015-05-05

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  1494
<212>  DNA
<213>  Pseudomonas sp. HZN6

<400>  1
atggctaacg ataagggtga tataagcaag gacggtgtat cgcgacgcaa atttcttggc       60

ggtgccgtta ttggtgctgc tgctgctgca ggcgttggct ctcagatcct atcactgtcc      120

gctacggcac agggggcgga taaagaaaga gttggtccgc tgcaaagcaa cgtagattac      180

gatgccgtcg tgatcggagg tgggtttgcg ggggtaacgg ctgcaaggga gttgagccga      240

tccggcttga aaacattagt gcttgagggc cggagtcgcc taggtggccg cacgtttacg      300

tctaagctcg atggtgagaa ggtggagctt ggaggtactt gggtacactg gacccagcct      360

aatgtgtgga ctgaggtcat gcattatgga ttagaaatcg aggaaaccgt cggtctcgct      420

agtcctgaaa ctgttatttg ggttactgat aatcaggtga agcgagcgcc ggcggcagag      480

gcgttcgaaa tatttggcgc cgcttgtact gaatattaca aagaagcgca caacatctac      540

ccacgtccct tcgatccttt ctttgcaaaa aaagcgctcc aggagatgga tgggttgtca      600

gcttctgagt acttaaataa actgtcccta acccgcgagc aaaaagacat gatggattca      660

tggcttagcg gtaatggaca taactaccca gagacgatcg cttatagcga gattatgcgc      720

tggtttgcac tcagcaactt caacatgccc actatgttcg actcaattgc cagatataaa      780

attaaatcag gtaccgtgag tcttctggag gccatggttg ctgaaagtga tatggaagtt      840

cagctttcaa cgcctgtgct aaaagttaag caagacagtc atagggtact tatcaccact      900

gaagagggca caattgcggc atcggcagtt gtcatggcag tgcctttaaa cacgatgggt      960

gacgttgagt acagtccgcg tctctctgat gcaaagtcag aaattgcctc ccaaggccat     1020

gcgggtaagg gtgttaaggg ttacattcgt ataaagcaag atgtaggtaa cgtaatgacc     1080

tatgcgcccg ctagaaacga tgtaacgcct ttcacttcgg tgtttacgga ccacgtcggt     1140

gagaacggta cattgcttat cgcattttcc gccgatccta aactggtaga cattaacgat     1200

agcaaagcgg tcgaaaaggc cctgcatccc cttcttccag gcgtggaggt aacgtccagc     1260

tatggctacg actggaatct cgatcccttt tctaagggca cttggtgcac ttatcgtccg     1320

ggtcagacaa cccgctattt aaccgagctg cagaagcgcg aaggccggct cttcttcgcc     1380

ggttccgaca tggctaatgg ctggcgtggt tttatagacg gcgcgataga gagcggtcgc     1440

gaggtcgggt atcaggttgc tagctatctc aaggggaaaa atagcaatgc gtga           1494


<210>  2
<211>  1664
<212>  DNA
<213>  Pseudomonas sp. HZN6

<400>  2
ggatccaggg aagttggtga tagtttgatt cccaagtgct tttctaaaaa tccataccat       60

ggctaatgat aagggagata taagtaaaga tggtgtgagt aggaggaagt ttctcggtgg      120

tgctgtgata ggtgctgctg ctgctgcagg tgttggatca caaattttaa gtctctccgc      180

tactgcacag ggtgctgata aggaaagagt tggaccactt caaagtaatg tggattatga      240

tgcagttgtg attggaggtg gatttgctgg tgtgactgct gcaagagaat tatctagatc      300

aggtctcaaa acacttgttt tggagggaag aagtaggttg ggtggaagga ctttcacatc      360

caagttggat ggagaaaaag ttgagttagg tggaacttgg gtgcattgga cacaaccaaa      420

cgtttggact gaagtgatgc actatggtct cgagattgaa gagacagttg gacttgcttc      480

tccagagact gttatatggg tgacagataa tcaggttaag agggctccag ctgcagaagc      540

atttgagatt ttcggtgctg catgtactga atattacaaa gaggctcata acatctatcc      600

aaggcctttc gatccatttt tcgctaagaa agcattacaa gagatggatg gactcagtgc      660

ttccgagtac cttaataagt tatctctcac tagagaacag aaagatatga tggattcttg      720

gctttctggt aatggtcaca actatccaga aacaatagca tactctgaga tcatgagatg      780

gtttgctctt tcaaatttca acatgcctac tatgttcgat tcaatcgcta ggtataagat      840

aaaaagtggt acagtttccc ttttggaggc tatggtggca gaatctgata tggaggttca      900

actttcaact ccagttttga aggtgaaaca ggattctcat agagttctta tcactacaga      960

agagggtact attgctgcat cagctgttgt gatggcagtg ccattgaata caatgggaga     1020

tgttgaatac tctcctaggt tatcagatgc taagagtgag attgcatccc aaggtcacgc     1080

tggaaaggga gttaaaggat acatcagaat taagcaggat gttggaaatg tgatgacata     1140

cgctccagca aggaacgatg ttactccttt tacatctgtt ttcactgatc atgtgggtga     1200

aaatggtact cttctcatag cttttagtgc agatcctaaa cttgtggata tcaacgattc     1260

caaggctgtt gaaaaagcat tgcacccact tttgcctggt gttgaagtga cttcttcata     1320

tggatacgat tggaatcttg atccattttc taagggtact tggtgcacat atagacctgg     1380

acaaactaca aggtacctta cagaattgca gaaaagagag ggtaggcttt tctttgcagg     1440

aagtgatatg gctaacggtt ggagaggttt tattgatggt gctattgaat ccggtaggga     1500

ggttggttat caggttgctt catatctcaa gggaaagaat agtaacgcat aaaatctaag     1560

atgttttatc ttggttgatc attgtttaat actcctagat agatgggtat tcatctatct     1620

ttttaaaatt aattgtcagt acgagtgttt ctgagctcaa gctt                      1664


<210>  3
<211>  497
<212>  PRT
<213>  Pseudomonas sp. HZN6

<400>  3

Met Ala Asn Asp Lys Gly Asp Ile Ser Lys Asp Gly Val Ser Arg Arg 
1               5                   10                  15      


Lys Phe Leu Gly Gly Ala Val Ile Gly Ala Ala Ala Ala Ala Gly Val 
            20                  25                  30          


Gly Ser Gln Ile Leu Ser Leu Ser Ala Thr Ala Gln Gly Ala Asp Lys 
        35                  40                  45              


Glu Arg Val Gly Pro Leu Gln Ser Asn Val Asp Tyr Asp Ala Val Val 
    50                  55                  60                  


Ile Gly Gly Gly Phe Ala Gly Val Thr Ala Ala Arg Glu Leu Ser Arg 
65                  70                  75                  80  


Ser Gly Leu Lys Thr Leu Val Leu Glu Gly Arg Ser Arg Leu Gly Gly 
                85                  90                  95      


Arg Thr Phe Thr Ser Lys Leu Asp Gly Glu Lys Val Glu Leu Gly Gly 
            100                 105                 110         


Thr Trp Val His Trp Thr Gln Pro Asn Val Trp Thr Glu Val Met His 
        115                 120                 125             


Tyr Gly Leu Glu Ile Glu Glu Thr Val Gly Leu Ala Ser Pro Glu Thr 
    130                 135                 140                 


Val Ile Trp Val Thr Asp Asn Gln Val Lys Arg Ala Pro Ala Ala Glu 
145                 150                 155                 160 


Ala Phe Glu Ile Phe Gly Ala Ala Cys Thr Glu Tyr Tyr Lys Glu Ala 
                165                 170                 175     


His Asn Ile Tyr Pro Arg Pro Phe Asp Pro Phe Phe Ala Lys Lys Ala 
            180                 185                 190         


Leu Gln Glu Met Asp Gly Leu Ser Ala Ser Glu Tyr Leu Asn Lys Leu 
        195                 200                 205             


Ser Leu Thr Arg Glu Gln Lys Asp Met Met Asp Ser Trp Leu Ser Gly 
    210                 215                 220                 


Asn Gly His Asn Tyr Pro Glu Thr Ile Ala Tyr Ser Glu Ile Met Arg 
225                 230                 235                 240 


Trp Phe Ala Leu Ser Asn Phe Asn Met Pro Thr Met Phe Asp Ser Ile 
                245                 250                 255     


Ala Arg Tyr Lys Ile Lys Ser Gly Thr Val Ser Leu Leu Glu Ala Met 
            260                 265                 270         


Val Ala Glu Ser Asp Met Glu Val Gln Leu Ser Thr Pro Val Leu Lys 
        275                 280                 285             


Val Lys Gln Asp Ser His Arg Val Leu Ile Thr Thr Glu Glu Gly Thr 
    290                 295                 300                 


Ile Ala Ala Ser Ala Val Val Met Ala Val Pro Leu Asn Thr Met Gly 
305                 310                 315                 320 


Asp Val Glu Tyr Ser Pro Arg Leu Ser Asp Ala Lys Ser Glu Ile Ala 
                325                 330                 335     


Ser Gln Gly His Ala Gly Lys Gly Val Lys Gly Tyr Ile Arg Ile Lys 
            340                 345                 350         


Gln Asp Val Gly Asn Val Met Thr Tyr Ala Pro Ala Arg Asn Asp Val 
        355                 360                 365             


Thr Pro Phe Thr Ser Val Phe Thr Asp His Val Gly Glu Asn Gly Thr 
    370                 375                 380                 


Leu Leu Ile Ala Phe Ser Ala Asp Pro Lys Leu Val Asp Ile Asn Asp 
385                 390                 395                 400 


Ser Lys Ala Val Glu Lys Ala Leu His Pro Leu Leu Pro Gly Val Glu 
                405                 410                 415     


Val Thr Ser Ser Tyr Gly Tyr Asp Trp Asn Leu Asp Pro Phe Ser Lys 
            420                 425                 430         


Gly Thr Trp Cys Thr Tyr Arg Pro Gly Gln Thr Thr Arg Tyr Leu Thr 
        435                 440                 445             


Glu Leu Gln Lys Arg Glu Gly Arg Leu Phe Phe Ala Gly Ser Asp Met 
    450                 455                 460                 


Ala Asn Gly Trp Arg Gly Phe Ile Asp Gly Ala Ile Glu Ser Gly Arg 
465                 470                 475                 480 


Glu Val Gly Tyr Gln Val Ala Ser Tyr Leu Lys Gly Lys Asn Ser Asn 
                485                 490                 495     


Ala 
    


<210>  4
<211>  1746
<212>  DNA
<213>  Nicotiana tabacum

<400>  4
gaagcagaaa tacatacaac atgtttccgc tcataattct gatcagcttt tcacttgctt       60

ccttgtctga aactgctact ggagctgtta caaatctttc agcctgctta atcaaccaca      120

atgtccataa cttctctatt taccccacaa gtagaaatta ctttaacttg ctccacttct      180

cccttcaaaa tcttcgcttt gctgcacctt tcatgccgaa accaaccttc attatcctac      240

caagcagtaa ggaggagctc gtgagcacca ttttttgttg cagaaaagca tcttatgaaa      300

tcagagtaag gtgcggcgga cacagttacg aaggaacttc ttacgtttcc tttgacgctt      360

ctccattcgt gatcgttgac ttgatgaaat tagacgacgt ttcagtagat ttggattctg      420

aaacagcttg ggctcagggc ggcgcaacaa ttggccaaat ttattatgcc attgccaagg      480

taagtgacgt tcatgcattt tcagcaggtt cgggaccaac agtaggatct ggaggtcata      540

tttcaggtgg tggatttgga cttttatcta gaaaattcgg acttgctgct gataatgtcg      600

ttgatgctct tcttattgat gctgatggac ggttattaga ccgaaaagcc atgggcgaag      660

acgtgttttg ggcaatcaga ggtggcggcg gtggaaattg gggcattgtt tatgcctgga      720

aaattcgatt actcaaagtg cctaaaatcg taacaacttg tatgatctat aggcctggat      780

ccaaacaata cgtggctcaa atacttgaga aatggcaaat agttactcca aatttggtcg      840

atgattttac tctaggagta ctgctgagac ctgcagatct acccgcggat atgaaatatg      900

gtaatactac tcctattgaa atatttcccc aattcaatgc actttatttg ggtccaaaaa      960

ctgaagttct ttccatatcg aatgagacat ttccggagct aggcgttaag aatgatgagt     1020

gcaaggaaat gacttgggta gagtcagcac ttttcttctc cgaattagct gacgttaacg     1080

ggaactcgac tggtgatatc tcccgtctga aagaacgtta catggacgga aaaggttttt     1140

tcaaaggcaa aacggactac gtgaagaagc cagtttcaat ggatgggatg ctaacatttc     1200

ttgtggaact cgagaaaaac ccgaagggat atcttgtctt tgatccttat ggcggagcca     1260

tggacaagat tagtgatcaa gctattgctt tccctcatag aaaaggtaac cttttcgcga     1320

ttcagtatct agcacagtgg aatgaagagg acgattacat gagcgacgtt tacatggagt     1380

ggataagagg attttacaat acaatgacgc cctttgtttc aagctcgcca aggggagctt     1440

atatcaacta cttggatatg gatcttggag tgaatatggt cgacgactac ttattgcgaa     1500

atgctagtag cagtagtcct tcttcctctg ttgatgctgt ggagagagct agagcgtggg     1560

gtgagatgta tttcttgcat aactatgata ggttggttaa agctaagaca caaattgatc     1620

cactaaatgt ttttcgacat gaacagagta ttcctcctat gcttggttca acgcaagagc     1680

acaagtatag cagtgaatga gatttaaaat gtactacctt gagagagatt ccgttgttag     1740

ttttcc                                                                1746


<210>  5
<211>  559
<212>  PRT
<213>  Nicotiana tabacum

<400>  5

Met Phe Pro Leu Ile Ile Leu Ile Ser Phe Ser Leu Ala Ser Leu Ser 
1               5                   10                  15      


Glu Thr Ala Thr Gly Ala Val Thr Asn Leu Ser Ala Cys Leu Ile Asn 
            20                  25                  30          


His Asn Val His Asn Phe Ser Ile Tyr Pro Thr Ser Arg Asn Tyr Phe 
        35                  40                  45              


Asn Leu Leu His Phe Ser Leu Gln Asn Leu Arg Phe Ala Ala Pro Phe 
    50                  55                  60                  


Met Pro Lys Pro Thr Phe Ile Ile Leu Pro Ser Ser Lys Glu Glu Leu 
65                  70                  75                  80  


Val Ser Thr Ile Phe Cys Cys Arg Lys Ala Ser Tyr Glu Ile Arg Val 
                85                  90                  95      


Arg Cys Gly Gly His Ser Tyr Glu Gly Thr Ser Tyr Val Ser Phe Asp 
            100                 105                 110         


Ala Ser Pro Phe Val Ile Val Asp Leu Met Lys Leu Asp Asp Val Ser 
        115                 120                 125             


Val Asp Leu Asp Ser Glu Thr Ala Trp Ala Gln Gly Gly Ala Thr Ile 
    130                 135                 140                 


Gly Gln Ile Tyr Tyr Ala Ile Ala Lys Val Ser Asp Val His Ala Phe 
145                 150                 155                 160 


Ser Ala Gly Ser Gly Pro Thr Val Gly Ser Gly Gly His Ile Ser Gly 
                165                 170                 175     


Gly Gly Phe Gly Leu Leu Ser Arg Lys Phe Gly Leu Ala Ala Asp Asn 
            180                 185                 190         


Val Val Asp Ala Leu Leu Ile Asp Ala Asp Gly Arg Leu Leu Asp Arg 
        195                 200                 205             


Lys Ala Met Gly Glu Asp Val Phe Trp Ala Ile Arg Gly Gly Gly Gly 
    210                 215                 220                 


Gly Asn Trp Gly Ile Val Tyr Ala Trp Lys Ile Arg Leu Leu Lys Val 
225                 230                 235                 240 


Pro Lys Ile Val Thr Thr Cys Met Ile Tyr Arg Pro Gly Ser Lys Gln 
                245                 250                 255     


Tyr Val Ala Gln Ile Leu Glu Lys Trp Gln Ile Val Thr Pro Asn Leu 
            260                 265                 270         


Val Asp Asp Phe Thr Leu Gly Val Leu Leu Arg Pro Ala Asp Leu Pro 
        275                 280                 285             


Ala Asp Met Lys Tyr Gly Asn Thr Thr Pro Ile Glu Ile Phe Pro Gln 
    290                 295                 300                 


Phe Asn Ala Leu Tyr Leu Gly Pro Lys Thr Glu Val Leu Ser Ile Ser 
305                 310                 315                 320 


Asn Glu Thr Phe Pro Glu Leu Gly Val Lys Asn Asp Glu Cys Lys Glu 
                325                 330                 335     


Met Thr Trp Val Glu Ser Ala Leu Phe Phe Ser Glu Leu Ala Asp Val 
            340                 345                 350         


Asn Gly Asn Ser Thr Gly Asp Ile Ser Arg Leu Lys Glu Arg Tyr Met 
        355                 360                 365             


Asp Gly Lys Gly Phe Phe Lys Gly Lys Thr Asp Tyr Val Lys Lys Pro 
    370                 375                 380                 


Val Ser Met Asp Gly Met Leu Thr Phe Leu Val Glu Leu Glu Lys Asn 
385                 390                 395                 400 


Pro Lys Gly Tyr Leu Val Phe Asp Pro Tyr Gly Gly Ala Met Asp Lys 
                405                 410                 415     


Ile Ser Asp Gln Ala Ile Ala Phe Pro His Arg Lys Gly Asn Leu Phe 
            420                 425                 430         


Ala Ile Gln Tyr Leu Ala Gln Trp Asn Glu Glu Asp Asp Tyr Met Ser 
        435                 440                 445             


Asp Val Tyr Met Glu Trp Ile Arg Gly Phe Tyr Asn Thr Met Thr Pro 
    450                 455                 460                 


Phe Val Ser Ser Ser Pro Arg Gly Ala Tyr Ile Asn Tyr Leu Asp Met 
465                 470                 475                 480 


Asp Leu Gly Val Asn Met Val Asp Asp Tyr Leu Leu Arg Asn Ala Ser 
                485                 490                 495     


Ser Ser Ser Pro Ser Ser Ser Val Asp Ala Val Glu Arg Ala Arg Ala 
            500                 505                 510         


Trp Gly Glu Met Tyr Phe Leu His Asn Tyr Asp Arg Leu Val Lys Ala 
        515                 520                 525             


Lys Thr Gln Ile Asp Pro Leu Asn Val Phe Arg His Glu Gln Ser Ile 
    530                 535                 540                 


Pro Pro Met Leu Gly Ser Thr Gln Glu His Lys Tyr Ser Ser Glu 
545                 550                 555                 


<210>  6
<211>  1785
<212>  DNA
<213>  Artificial

<220>
<223>  BBLa-PAO fusion construct

<400>  6
ggatccgaag cagaaataca tacaacatgt ttccgctcat aattctgatc agcttttcac       60

ttgcttcctt gtctgaaact gctactggag ctgttacaaa tctttcagcc tgcttaatca      120

accacaatgt ccataacttc tctatttacc ccacaagtag aaattacttt aacttggcca      180

tggctaatga taagggagat ataagtaaag atggtgtgag taggaggaag tttctcggtg      240

gtgctgtgat aggtgctgct gctgctgcag gtgttggatc acaaatttta agtctctccg      300

ctactgcaca gggtgctgat aaggaaagag ttggaccact tcaaagtaat gtggattatg      360

atgcagttgt gattggaggt ggatttgctg gtgtgactgc tgcaagagaa ttatctagat      420

caggtctcaa aacacttgtt ttggagggaa gaagtaggtt gggtggaagg actttcacat      480

ccaagttgga tggagaaaaa gttgagttag gtggaacttg ggtgcattgg acacaaccaa      540

acgtttggac tgaagtgatg cactatggtc tcgagattga agagacagtt ggacttgctt      600

ctccagagac tgttatatgg gtgacagata atcaggttaa gagggctcca gctgcagaag      660

catttgagat tttcggtgct gcatgtactg aatattacaa agaggctcat aacatctatc      720

caaggccttt cgatccattt ttcgctaaga aagcattaca agagatggat ggactcagtg      780

cttccgagta ccttaataag ttatctctca ctagagaaca gaaagatatg atggattctt      840

ggctttctgg taatggtcac aactatccag aaacaatagc atactctgag atcatgagat      900

ggtttgctct ttcaaatttc aacatgccta ctatgttcga ttcaatcgct aggtataaga      960

taaaaagtgg tacagtttcc cttttggagg ctatggtggc agaatctgat atggaggttc     1020

aactttcaac tccagttttg aaggtgaaac aggattctca tagagttctt atcactacag     1080

aagagggtac tattgctgca tcagctgttg tgatggcagt gccattgaat acaatgggag     1140

atgttgaata ctctcctagg ttatcagatg ctaagagtga gattgcatcc caaggtcacg     1200

ctggaaaggg agttaaagga tacatcagaa ttaagcagga tgttggaaat gtgatgacat     1260

acgctccagc aaggaacgat gttactcctt ttacatctgt tttcactgat catgtgggtg     1320

aaaatggtac tcttctcata gcttttagtg cagatcctaa acttgtggat atcaacgatt     1380

ccaaggctgt tgaaaaagca ttgcacccac ttttgcctgg tgttgaagtg acttcttcat     1440

atggatacga ttggaatctt gatccatttt ctaagggtac ttggtgcaca tatagacctg     1500

gacaaactac aaggtacctt acagaattgc agaaaagaga gggtaggctt ttctttgcag     1560

gaagtgatat ggctaacggt tggagaggtt ttattgatgg tgctattgaa tccggtaggg     1620

aggttggtta tcaggttgct tcatatctca agggaaagaa tagtaacgca taaaatctaa     1680

gatgttttat cttggttgat cattgtttaa tactcctaga tagatgggta ttcatctatc     1740

tttttaaaat taattgtcag tacgagtgtt tctgagctca agctt                     1785


<210>  7
<211>  548
<212>  PRT
<213>  Artificial

<220>
<223>  BBLa-PAO fusion construct

<400>  7

Met Phe Pro Leu Ile Ile Leu Ile Ser Phe Ser Leu Ala Ser Leu Ser 
1               5                   10                  15      


Glu Thr Ala Thr Gly Ala Val Thr Asn Leu Ser Ala Cys Leu Ile Asn 
            20                  25                  30          


His Asn Val His Asn Phe Ser Ile Tyr Pro Thr Ser Arg Asn Tyr Phe 
        35                  40                  45              


Asn Leu Ala Met Ala Asn Asp Lys Gly Asp Ile Ser Lys Asp Gly Val 
    50                  55                  60                  


Ser Arg Arg Lys Phe Leu Gly Gly Ala Val Ile Gly Ala Ala Ala Ala 
65                  70                  75                  80  


Ala Gly Val Gly Ser Gln Ile Leu Ser Leu Ser Ala Thr Ala Gln Gly 
                85                  90                  95      


Ala Asp Lys Glu Arg Val Gly Pro Leu Gln Ser Asn Val Asp Tyr Asp 
            100                 105                 110         


Ala Val Val Ile Gly Gly Gly Phe Ala Gly Val Thr Ala Ala Arg Glu 
        115                 120                 125             


Leu Ser Arg Ser Gly Leu Lys Thr Leu Val Leu Glu Gly Arg Ser Arg 
    130                 135                 140                 


Leu Gly Gly Arg Thr Phe Thr Ser Lys Leu Asp Gly Glu Lys Val Glu 
145                 150                 155                 160 


Leu Gly Gly Thr Trp Val His Trp Thr Gln Pro Asn Val Trp Thr Glu 
                165                 170                 175     


Val Met His Tyr Gly Leu Glu Ile Glu Glu Thr Val Gly Leu Ala Ser 
            180                 185                 190         


Pro Glu Thr Val Ile Trp Val Thr Asp Asn Gln Val Lys Arg Ala Pro 
        195                 200                 205             


Ala Ala Glu Ala Phe Glu Ile Phe Gly Ala Ala Cys Thr Glu Tyr Tyr 
    210                 215                 220                 


Lys Glu Ala His Asn Ile Tyr Pro Arg Pro Phe Asp Pro Phe Phe Ala 
225                 230                 235                 240 


Lys Lys Ala Leu Gln Glu Met Asp Gly Leu Ser Ala Ser Glu Tyr Leu 
                245                 250                 255     


Asn Lys Leu Ser Leu Thr Arg Glu Gln Lys Asp Met Met Asp Ser Trp 
            260                 265                 270         


Leu Ser Gly Asn Gly His Asn Tyr Pro Glu Thr Ile Ala Tyr Ser Glu 
        275                 280                 285             


Ile Met Arg Trp Phe Ala Leu Ser Asn Phe Asn Met Pro Thr Met Phe 
    290                 295                 300                 


Asp Ser Ile Ala Arg Tyr Lys Ile Lys Ser Gly Thr Val Ser Leu Leu 
305                 310                 315                 320 


Glu Ala Met Val Ala Glu Ser Asp Met Glu Val Gln Leu Ser Thr Pro 
                325                 330                 335     


Val Leu Lys Val Lys Gln Asp Ser His Arg Val Leu Ile Thr Thr Glu 
            340                 345                 350         


Glu Gly Thr Ile Ala Ala Ser Ala Val Val Met Ala Val Pro Leu Asn 
        355                 360                 365             


Thr Met Gly Asp Val Glu Tyr Ser Pro Arg Leu Ser Asp Ala Lys Ser 
    370                 375                 380                 


Glu Ile Ala Ser Gln Gly His Ala Gly Lys Gly Val Lys Gly Tyr Ile 
385                 390                 395                 400 


Arg Ile Lys Gln Asp Val Gly Asn Val Met Thr Tyr Ala Pro Ala Arg 
                405                 410                 415     


Asn Asp Val Thr Pro Phe Thr Ser Val Phe Thr Asp His Val Gly Glu 
            420                 425                 430         


Asn Gly Thr Leu Leu Ile Ala Phe Ser Ala Asp Pro Lys Leu Val Asp 
        435                 440                 445             


Ile Asn Asp Ser Lys Ala Val Glu Lys Ala Leu His Pro Leu Leu Pro 
    450                 455                 460                 


Gly Val Glu Val Thr Ser Ser Tyr Gly Tyr Asp Trp Asn Leu Asp Pro 
465                 470                 475                 480 


Phe Ser Lys Gly Thr Trp Cys Thr Tyr Arg Pro Gly Gln Thr Thr Arg 
                485                 490                 495     


Tyr Leu Thr Glu Leu Gln Lys Arg Glu Gly Arg Leu Phe Phe Ala Gly 
            500                 505                 510         


Ser Asp Met Ala Asn Gly Trp Arg Gly Phe Ile Asp Gly Ala Ile Glu 
        515                 520                 525             


Ser Gly Arg Glu Val Gly Tyr Gln Val Ala Ser Tyr Leu Lys Gly Lys 
    530                 535                 540                 


Asn Ser Asn Ala 
545             


<210>  8
<211>  170
<212>  DNA
<213>  Nicotiana tabacum

<400>  8
gaagcagaaa tacatacaac atgtttccgc tcataattct gatcagcttt tcacttgctt       60

ccttgtctga aactgctact ggagctgtta caaatctttc agcctgctta atcaaccaca      120

atgtccataa cttctctatt taccccacaa gtagaaatta ctttaacttg                 170


<210>  9
<211>  1491
<212>  DNA
<213>  Pseudomonas putida S16

<400>  9
atgacaaaag atggtgatga aggcagcaaa agcggagtat cacgccgaaa gttccttggt       60

agcgccgcgg tcggagtggc aacagcgggc atagcctcgc agcttctgac tctgtcggcg      120

cccgcggaag cggcggtgaa gaccaatgtt ggtccatcac gcgcaggcgt gggttatgac      180

gttattgtaa tcggtggagg cttcgcgggt gttactgcgg cgcgagaagc aagccgttct      240

ggcttgaaaa ctctaattct tgaaggtaga agtcggttgg gcggccgaac ttttacgtct      300

aagcttcaga atcaaaaagt tgagttggga ggtacctggg tacattggac ccagccgaat      360

gtttggactg agattatgca ctatggccta gaggtggagg agacggtcgg tcttgcgaat      420

ccagagaccg tcatttgggt aacagaggat aatgtcaaaa gagcacctgc agcagaggcg      480

tttgaaattt ttggctctgc ctgtaacgaa tactacaaag aagcacggaa tatttatccg      540

cgcccgtttg aaccattttt tgagcgaaag aagctgcagc atgttgatgg actttccgcg      600

gcagattatc ttgaaaaatt gcctttgacc agggagcaaa aggatatgat ggattcctgg      660

ctcagtggaa atggacataa ttatccggaa actattgcat atagcgagat tatgcgttgg      720

tttgcactca gtaactttaa tatgcccaca atgttcgatt ccattgcgcg gtacaaaatt      780

aaaaccggta cgcatagtct tttagaggcc ataatggcgg atggtaactc ggaagttaaa      840

ctttcgacgc cagtgactaa ggttaatcaa gataaagata aagtaacagt taccactgaa      900

gacggtgtct tcacggcatc agcggtaatt gtagccgtcc ctatcaacac tttgcatgat      960

attgagtact cgccgaagtt gtcggcagct aaagtggata tgggatcgca acggcatgct     1020

ggtgctggtg taaagggcta tattcgcgta aaacaaaatg tcggcaatgt aatgacatat     1080

gctcctgccc ggaataagct cacaccattt acctcagtat tcacagatca cgtggacgag     1140

agcggtacgt tactcattgc attttcagcc gaccctaagt tgattgatat caatgatatc     1200

aaagccgtcg aaaaggcttt gcaaccactt ttgccaggcg tcgaagtaac tgctagttat     1260

ggctacgact ggaacctcga tcccttttct aagggcactt ggtgcactta ccgtcctaac     1320

cagacgactc gatacttgac tgagctgcag aagcgcgaag gacggctatt ttttgcaggc     1380

tcggacatgg ccaatggttg gcgtggattc attgatggag caatcgagaa cggtagagag     1440

gtagggcatc aagtcgctac gtatctaaaa agagaaaatg acaatgcgtg a              1491


<210>  10
<211>  496
<212>  PRT
<213>  Pseudomonas putida S16

<400>  10

Met Thr Lys Asp Gly Asp Glu Gly Ser Lys Ser Gly Val Ser Arg Arg 
1               5                   10                  15      


Lys Phe Leu Gly Ser Ala Ala Val Gly Val Ala Thr Ala Gly Ile Ala 
            20                  25                  30          


Ser Gln Leu Leu Thr Leu Ser Ala Pro Ala Glu Ala Ala Val Lys Thr 
        35                  40                  45              


Asn Val Gly Pro Ser Arg Ala Gly Val Gly Tyr Asp Val Ile Val Ile 
    50                  55                  60                  


Gly Gly Gly Phe Ala Gly Val Thr Ala Ala Arg Glu Ala Ser Arg Ser 
65                  70                  75                  80  


Gly Leu Lys Thr Leu Ile Leu Glu Gly Arg Ser Arg Leu Gly Gly Arg 
                85                  90                  95      


Thr Phe Thr Ser Lys Leu Gln Asn Gln Lys Val Glu Leu Gly Gly Thr 
            100                 105                 110         


Trp Val His Trp Thr Gln Pro Asn Val Trp Thr Glu Ile Met His Tyr 
        115                 120                 125             


Gly Leu Glu Val Glu Glu Thr Val Gly Leu Ala Asn Pro Glu Thr Val 
    130                 135                 140                 


Ile Trp Val Thr Glu Asp Asn Val Lys Arg Ala Pro Ala Ala Glu Ala 
145                 150                 155                 160 


Phe Glu Ile Phe Gly Ser Ala Cys Asn Glu Tyr Tyr Lys Glu Ala Arg 
                165                 170                 175     


Asn Ile Tyr Pro Arg Pro Phe Glu Pro Phe Phe Glu Arg Lys Lys Leu 
            180                 185                 190         


Gln His Val Asp Gly Leu Ser Ala Ala Asp Tyr Leu Glu Lys Leu Pro 
        195                 200                 205             


Leu Thr Arg Glu Gln Lys Asp Met Met Asp Ser Trp Leu Ser Gly Asn 
    210                 215                 220                 


Gly His Asn Tyr Pro Glu Thr Ile Ala Tyr Ser Glu Ile Met Arg Trp 
225                 230                 235                 240 


Phe Ala Leu Ser Asn Phe Asn Met Pro Thr Met Phe Asp Ser Ile Ala 
                245                 250                 255     


Arg Tyr Lys Ile Lys Thr Gly Thr His Ser Leu Leu Glu Ala Ile Met 
            260                 265                 270         


Ala Asp Gly Asn Ser Glu Val Lys Leu Ser Thr Pro Val Thr Lys Val 
        275                 280                 285             


Asn Gln Asp Lys Asp Lys Val Thr Val Thr Thr Glu Asp Gly Val Phe 
    290                 295                 300                 


Thr Ala Ser Ala Val Ile Val Ala Val Pro Ile Asn Thr Leu His Asp 
305                 310                 315                 320 


Ile Glu Tyr Ser Pro Lys Leu Ser Ala Ala Lys Val Asp Met Gly Ser 
                325                 330                 335     


Gln Arg His Ala Gly Ala Gly Val Lys Gly Tyr Ile Arg Val Lys Gln 
            340                 345                 350         


Asn Val Gly Asn Val Met Thr Tyr Ala Pro Ala Arg Asn Lys Leu Thr 
        355                 360                 365             


Pro Phe Thr Ser Val Phe Thr Asp His Val Asp Glu Ser Gly Thr Leu 
    370                 375                 380                 


Leu Ile Ala Phe Ser Ala Asp Pro Lys Leu Ile Asp Ile Asn Asp Ile 
385                 390                 395                 400 


Lys Ala Val Glu Lys Ala Leu Gln Pro Leu Leu Pro Gly Val Glu Val 
                405                 410                 415     


Thr Ala Ser Tyr Gly Tyr Asp Trp Asn Leu Asp Pro Phe Ser Lys Gly 
            420                 425                 430         


Thr Trp Cys Thr Tyr Arg Pro Asn Gln Thr Thr Arg Tyr Leu Thr Glu 
        435                 440                 445             


Leu Gln Lys Arg Glu Gly Arg Leu Phe Phe Ala Gly Ser Asp Met Ala 
    450                 455                 460                 


Asn Gly Trp Arg Gly Phe Ile Asp Gly Ala Ile Glu Asn Gly Arg Glu 
465                 470                 475                 480 


Val Gly His Gln Val Ala Thr Tyr Leu Lys Arg Glu Asn Asp Asn Ala 
                485                 490                 495     


