                         SEQUENCE LISTING

<110>  Pieris AG
 
<120>  Novel proteins specific for CD137

<130>  PIE15347PCT

<150>  EP15166184.0
<151>  2015-05-04


<160>  39    

<170>  PatentIn version 3.5


<210>  1
<211>  156
<212>  PRT
<213>  artificial

<220>
<223>  wildtype human Tlc

<400>  1

His His Leu Leu Ala Ser Asp Glu Glu Ile Gln Asp Val Ser Gly Thr 
1               5                   10                  15      


Trp Tyr Leu Lys Ala Met Thr Val Asp Arg Glu Phe Pro Glu Met Asn 
            20                  25                  30          


Leu Glu Ser Val Thr Pro Met Thr Leu Thr Thr Leu Glu Gly Gly Asn 
        35                  40                  45              


Leu Glu Ala Lys Val Thr Met Leu Ile Ser Gly Arg Cys Gln Glu Val 
    50                  55                  60                  


Lys Ala Val Leu Glu Lys Thr Asp Glu Pro Gly Lys Tyr Thr Ala Asp 
65                  70                  75                  80  


Gly Gly Lys His Val Ala Tyr Ile Ile Arg Ser His Val Lys Asp His 
                85                  90                  95      


Tyr Ile Phe Tyr Cys Glu Gly Glu Leu His Gly Lys Pro Val Arg Gly 
            100                 105                 110         


Val Lys Leu Val Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala Leu Glu 
        115                 120                 125             


Asp Phe Glu Lys Ala Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser Ile 
    130                 135                 140                 


Leu Ile Pro Arg Gln Ser Glu Thr Cys Ser Pro Gly 
145                 150                 155     


<210>  2
<211>  178
<212>  PRT
<213>  artificial

<220>
<223>  wildtype hNGAL

<400>  2

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe Gln Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Leu Ala Gly Asn Ala Ile Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              


Gln Lys Met Tyr Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  


Asn Val Thr Ser Val Leu Phe Arg Lys Lys Lys Cys Asp Tyr Trp Ile 
65                  70                  75                  80  


Arg Thr Phe Val Pro Gly Cys Gln Pro Gly Glu Phe Thr Leu Gly Asn 
                85                  90                  95      


Ile Lys Ser Tyr Pro Gly Leu Thr Ser Tyr Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Ser Gln 
        115                 120                 125             


Asn Arg Glu Tyr Phe Lys Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 


Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 


Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     


Asp Gly 
        


<210>  3
<211>  152
<212>  PRT
<213>  artificial

<220>
<223>  negative control 

<400>  3

Ala Ser Asp Glu Glu Ile Gln Asp Val Ser Gly Thr Trp Tyr Leu Lys 
1               5                   10                  15      


Ala Met Thr Val Asp Arg Glu Cys Pro Glu Met Asn Leu Glu Ser Val 
            20                  25                  30          


Thr Pro Met Thr Leu Thr Thr Leu Glu Gly Gly Asn Leu Glu Ala Lys 
        35                  40                  45              


Val Thr Met Leu Ile Ser Gly Arg Ser Gln Glu Val Lys Ala Val Leu 
    50                  55                  60                  


Glu Lys Thr Asp Glu Pro Gly Lys Tyr Thr Ala Asp Gly Gly Lys His 
65                  70                  75                  80  


Val Ala Tyr Ile Ile Arg Ser His Val Lys Asp His Tyr Ile Phe Tyr 
                85                  90                  95      


Ser Glu Gly Glu Cys His Gly Lys Pro Val Pro Gly Val Trp Leu Val 
            100                 105                 110         


Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala Leu Glu Asp Phe Glu Lys 
        115                 120                 125             


Ala Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser Ile Leu Ile Pro Arg 
    130                 135                 140                 


Gln Ser Glu Thr Ser Ser Pro Gly 
145                 150         


<210>  4
<211>  178
<212>  PRT
<213>  artificial

<220>
<223>  negative control 

<400>  4

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Leu Ala Gly Asn Ala Ile Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              


Gln Lys Met Tyr Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  


Asn Val Thr Ser Val Leu Phe Arg Lys Lys Lys Cys Asp Tyr Trp Ile 
65                  70                  75                  80  


Arg Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 
                85                  90                  95      


Ile Lys Ser Tyr Pro Gly Leu Thr Ser Tyr Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Ser Gln 
        115                 120                 125             


Asn Arg Glu Tyr Phe Lys Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 


Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 


Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     


Asp Gly 
        


<210>  5
<211>  152
<212>  PRT
<213>  artificial

<220>
<223>  lipocalin mutein 

<400>  5

Ala Ser Asp Glu Glu Ile Gln Asp Val Ser Gly Thr Trp Tyr Leu Lys 
1               5                   10                  15      


Ala Met Thr Val Asp Glu Gly Cys Arg Pro Trp Asn Ile Phe Ser Val 
            20                  25                  30          


Thr Pro Met Thr Leu Thr Thr Leu Glu Gly Gly Asn Leu Glu Ala Lys 
        35                  40                  45              


Val Thr Met Ala Ile Asp Gly Pro Ala Gln Glu Val Lys Ala Val Leu 
    50                  55                  60                  


Glu Lys Thr Asp Glu Pro Gly Lys Tyr Thr Ala Asp Gly Gly Lys His 
65                  70                  75                  80  


Val Ala Tyr Ile Ile Arg Ser His Val Lys Asp His Tyr Ile Phe Tyr 
                85                  90                  95      


Ser Glu Gly Val Cys Asp Gly Ser Pro Val Pro Gly Val Trp Leu Val 
            100                 105                 110         


Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala Leu Glu Asp Phe Glu Lys 
        115                 120                 125             


Ala Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser Ile Leu Ile Pro Arg 
    130                 135                 140                 


Gln Ser Glu Thr Ser Ser Pro Gly 
145                 150         


<210>  6
<211>  152
<212>  PRT
<213>  artificial

<220>
<223>  lipocalin mutein 

<400>  6

Thr Ser Asp Glu Glu Ile Gln Asp Val Ser Gly Thr Trp Tyr Leu Lys 
1               5                   10                  15      


Ala Met Thr Val Asp Glu Gly Cys Arg Pro Trp Asn Ile Phe Ser Val 
            20                  25                  30          


Thr Pro Met Thr Leu Thr Thr Leu Glu Gly Gly Asn Leu Glu Ala Lys 
        35                  40                  45              


Val Thr Met Ala Ile Asp Gly Pro Ala Gln Glu Val Arg Ala Val Leu 
    50                  55                  60                  


Glu Lys Thr Asp Glu Pro Gly Lys Tyr Thr Ala Asp Gly Gly Lys His 
65                  70                  75                  80  


Asp Ala Tyr Ile Ile Arg Ser His Val Lys Asp His Tyr Ile Phe Tyr 
                85                  90                  95      


Ser Glu Gly Val Cys Asp Gly Ser Pro Val Pro Gly Val Trp Leu Val 
            100                 105                 110         


Gly Arg Asp Pro Glu Asn Asn Leu Glu Ala Leu Glu Asp Phe Glu Lys 
        115                 120                 125             


Thr Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser Ile Leu Ile Pro Arg 
    130                 135                 140                 


Gln Ser Glu Thr Ser Ser Pro Gly 
145                 150         


<210>  7
<211>  152
<212>  PRT
<213>  artificial

<220>
<223>  lipocalin mutein 

<400>  7

Ala Ser Asp Glu Glu Ile Gln Asp Val Ser Gly Thr Trp Tyr Leu Lys 
1               5                   10                  15      


Ala Met Thr Val Asp Glu Gly Cys Arg Pro Trp Asn Ile Phe Ser Val 
            20                  25                  30          


Thr Pro Met Thr Leu Thr Thr Leu Glu Gly Gly Asn Leu Glu Ala Lys 
        35                  40                  45              


Val Thr Met Ala Ile Asp Gly Pro Ala Gln Glu Val Asn Ala Val Leu 
    50                  55                  60                  


Glu Lys Thr Asp Glu Pro Gly Lys Tyr Thr Ala Asp Gly Gly Lys His 
65                  70                  75                  80  


Val Ala Tyr Ile Ile Arg Ser His Val Arg Asp His Tyr Ile Phe Tyr 
                85                  90                  95      


Ser Glu Gly Val Cys Asp Gly Ser Pro Val Pro Gly Val Trp Leu Val 
            100                 105                 110         


Gly Arg Asp Pro Glu Asn Asn Leu Glu Ala Leu Glu Asp Phe Glu Lys 
        115                 120                 125             


Thr Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser Ile Leu Ile Pro Arg 
    130                 135                 140                 


Gln Ser Glu Thr Ser Ser Pro Gly 
145                 150         


<210>  8
<211>  152
<212>  PRT
<213>  artificial

<220>
<223>  lipocalin mutein 

<400>  8

Val Ser Asp Glu Glu Ile Gln Asp Val Ser Gly Thr Trp Tyr Leu Lys 
1               5                   10                  15      


Ala Met Thr Val Asp Glu Gly Cys Arg Pro Trp Asn Ile Phe Ser Val 
            20                  25                  30          


Thr Pro Met Thr Leu Thr Thr Leu Glu Gly Gly Asn Leu Glu Ala Lys 
        35                  40                  45              


Val Thr Met Ala Ile Asp Gly Pro Ala Gln Glu Val Arg Ala Val Leu 
    50                  55                  60                  


Glu Lys Thr Asp Glu Pro Gly Lys Tyr Thr Ala Asp Gly Gly Lys His 
65                  70                  75                  80  


Val Ala Tyr Ile Ile Arg Ser His Val Glu Asp His Tyr Ile Phe Tyr 
                85                  90                  95      


Ser Glu Gly Val Cys Asp Gly Ser Pro Val Pro Gly Val Trp Leu Val 
            100                 105                 110         


Gly Arg Asp Pro Glu Asn Asn Leu Glu Ala Leu Glu Asp Phe Glu Lys 
        115                 120                 125             


Thr Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser Ile Leu Ile Pro Arg 
    130                 135                 140                 


Gln Ser Glu Thr Ser Ser Pro Gly 
145                 150         


<210>  9
<211>  152
<212>  PRT
<213>  artificial

<220>
<223>  lipocalin mutein 

<400>  9

Ala Ser Asp Glu Glu Ile Gln Asp Val Ser Gly Thr Trp Tyr Leu Lys 
1               5                   10                  15      


Ala Met Thr Val Asp Glu Gly Cys Arg Pro Trp Asn Ile Phe Ser Val 
            20                  25                  30          


Thr Pro Met Thr Leu Ser Thr Leu Glu Gly Gly Asn Leu Glu Ala Lys 
        35                  40                  45              


Val Thr Met Ala Ile Asp Gly Pro Ala Gln Glu Val Lys Ala Val Leu 
    50                  55                  60                  


Glu Lys Thr Asp Glu Pro Gly Lys Tyr Thr Ala Asp Gly Gly Lys His 
65                  70                  75                  80  


Val Ala Tyr Ile Ile Arg Ser His Val Lys Asp His Tyr Ile Phe Tyr 
                85                  90                  95      


Ser Glu Gly Val Cys Asp Gly Ser Pro Val Pro Gly Val Trp Leu Val 
            100                 105                 110         


Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala Leu Glu Asp Phe Glu Lys 
        115                 120                 125             


Ala Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser Ile Leu Ile Pro Arg 
    130                 135                 140                 


Gln Ile Glu Thr Ser Ser Pro Gly 
145                 150         


<210>  10
<211>  152
<212>  PRT
<213>  artificial

<220>
<223>  lipocalin mutein 

<400>  10

Ala Ser Asp Glu Glu Ile Gln Asp Val Ser Gly Thr Trp Tyr Leu Lys 
1               5                   10                  15      


Ala Met Thr Val Asp Glu Gly Cys Arg Pro Trp Asn Ile Phe Ser Val 
            20                  25                  30          


Thr Pro Met Thr Leu Thr Thr Leu Glu Gly Gly Asn Leu Glu Ala Glu 
        35                  40                  45              


Val Thr Met Ala Ile Asp Gly Pro Ala Gln Glu Val Lys Ala Val Leu 
    50                  55                  60                  


Glu Lys Ala Asp Glu Pro Gly Lys Tyr Thr Ala Asp Gly Gly Lys His 
65                  70                  75                  80  


Val Ala Tyr Ile Ile Arg Ser His Val Lys Asp His Tyr Ile Phe Tyr 
                85                  90                  95      


Ser Glu Gly Val Cys Asp Gly Ser Pro Val Pro Gly Val Trp Leu Val 
            100                 105                 110         


Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala Leu Glu Asp Phe Glu Lys 
        115                 120                 125             


Thr Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser Ile Leu Ile Pro Ser 
    130                 135                 140                 


Gln Ile Glu Thr Ser Ser Pro Gly 
145                 150         


<210>  11
<211>  152
<212>  PRT
<213>  artificial

<220>
<223>  lipocalin mutein 

<400>  11

Thr Ser Asp Glu Glu Ile Gln Asp Val Ser Gly Thr Trp Tyr Leu Lys 
1               5                   10                  15      


Ala Met Thr Val Asp Glu Gly Cys Arg Pro Trp Asn Ile Phe Ser Val 
            20                  25                  30          


Thr Pro Met Thr Leu Thr Thr Leu Glu Asp Gly Asn Leu Glu Ala Lys 
        35                  40                  45              


Val Thr Met Ala Ile Asp Gly Pro Ala Gln Glu Val Lys Ala Val Leu 
    50                  55                  60                  


Glu Lys Ala Asp Glu Pro Gly Lys Tyr Thr Ala Asp Gly Gly Lys His 
65                  70                  75                  80  


Val Ala Tyr Ile Ile Arg Ser His Val Lys Asp His Tyr Ile Phe Tyr 
                85                  90                  95      


Ser Glu Gly Val Cys Asp Gly Ser Pro Val Pro Gly Val Trp Leu Val 
            100                 105                 110         


Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala Leu Glu Asp Phe Glu Lys 
        115                 120                 125             


Ala Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser Ile Leu Ile Pro Arg 
    130                 135                 140                 


Gln Ile Glu Thr Ser Ser Pro Gly 
145                 150         


<210>  12
<211>  178
<212>  PRT
<213>  artificial

<220>
<223>  lipocalin mutein 

<400>  12

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Gln Ala Gly Asn Ile Lys Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              


Asn Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  


Asn Val Thr Gly Val Thr Phe Asp Asp Lys Lys Cys Thr Tyr Ala Ile 
65                  70                  75                  80  


Ser Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Lys 
                85                  90                  95      


Ile Lys Ser Phe Pro Gly His Thr Ser Ser Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Phe Val Phe Gln 
        115                 120                 125             


Asn Arg Glu Glu Phe Tyr Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 


Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 


Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     


Asp Gly 
        


<210>  13
<211>  178
<212>  PRT
<213>  artificial

<220>
<223>  lipocalin mutein 

<400>  13

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Gln Ala Gly Asn Ile Arg Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              


Ile Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  


Asp Val Thr Met Val Lys Phe Asp Asp Lys Lys Cys Met Tyr Asp Ile 
65                  70                  75                  80  


Trp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Lys 
                85                  90                  95      


Ile Lys Ser Phe Pro Gly His Thr Ser Ser Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Phe Val Phe Gln 
        115                 120                 125             


Asn Arg Glu Glu Phe Tyr Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 


Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 


Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     


Asp Gly 
        


<210>  14
<211>  178
<212>  PRT
<213>  artificial

<220>
<223>  lipocalin mutein 

<400>  14

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Gln Ala Gly Asn Ile Arg Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              


Asn Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  


Asp Val Thr Ala Val Ala Phe Asp Asp Lys Lys Cys Thr Tyr Asp Ile 
65                  70                  75                  80  


Trp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Lys 
                85                  90                  95      


Ile Lys Ser Phe Pro Gly His Thr Ser Ser Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Phe Val Phe Gln 
        115                 120                 125             


Asn Arg Glu Glu Phe Tyr Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 


Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 


Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     


Asp Gly 
        


<210>  15
<211>  178
<212>  PRT
<213>  artificial

<220>
<223>  lipocalin muein 

<400>  15

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Gln Ala Gly Asn Ile Lys Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              


Asn Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  


Asp Val Thr Ala Val Ala Phe Asp Asp Lys Lys Cys Thr Tyr Asp Ile 
65                  70                  75                  80  


Trp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Lys 
                85                  90                  95      


Ile Lys Ser Phe Pro Gly His Thr Ser Ser Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Phe Val Phe Gln 
        115                 120                 125             


Asn Arg Glu Glu Phe Tyr Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 


Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 


Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     


Asp Gly 
        


<210>  16
<211>  175
<212>  PRT
<213>  artificial

<220>
<223>  lipocalin mutein 

<400>  16

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Gln Ala Gly Asn Ile Lys Leu Arg Glu Asp Ser Lys Met 
        35                  40                  45              


Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr 
    50                  55                  60                  


Gly Val Ser Phe Asp Asp Lys Lys Cys Thr Tyr Ala Ile Met Thr Phe 
65                  70                  75                  80  


Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Lys Ile Lys Ser 
                85                  90                  95      


Phe Pro Gly His Thr Ser Ser Leu Val Arg Val Val Ser Thr Asn Tyr 
            100                 105                 110         


Asn Gln His Ala Met Val Phe Phe Lys Phe Val Phe Gln Asn Arg Glu 
        115                 120                 125             


Glu Phe Tyr Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu 
    130                 135                 140                 


Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu 
145                 150                 155                 160 


Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly 
                165                 170                 175 


<210>  17
<211>  178
<212>  PRT
<213>  artificial

<220>
<223>  lipocalin mutein 

<400>  17

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Gln Ala Gly Asn Ile Lys Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              


Val Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  


Asp Val Thr Gly Val Thr Phe Asp Asp Lys Lys Cys Arg Tyr Asp Ile 
65                  70                  75                  80  


Ser Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Phe Gly Lys 
                85                  90                  95      


Ile Lys Ser Phe Pro Gly His Thr Ser Ser Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Phe Val Phe Gln 
        115                 120                 125             


Asn Arg Glu Glu Phe Tyr Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 


Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 


Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     


Asp Gly 
        


<210>  18
<211>  178
<212>  PRT
<213>  artificial

<220>
<223>  lipocalin mutein 

<400>  18

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Gln Ala Gly Asn Ile Arg Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              


His Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  


Asp Val Thr Gly Val Thr Phe Asp Asp Lys Lys Cys Thr Tyr Ala Ile 
65                  70                  75                  80  


Ser Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Lys 
                85                  90                  95      


Ile Lys Ser Phe Pro Gly His Thr Ser Ser Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Phe Val Phe Gln 
        115                 120                 125             


Asn Arg Glu Glu Phe Tyr Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 


Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 


Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     


Asp Gly 
        


<210>  19
<211>  178
<212>  PRT
<213>  artificial

<220>
<223>  lipocalin mutein 

<400>  19

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Gln Ala Gly Asn Ile Lys Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              


Asn Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  


Asp Val Thr Gly Val Thr Phe Asp Asp Lys Lys Cys Thr Tyr Ala Ile 
65                  70                  75                  80  


Ser Thr Leu Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Phe Gly Lys 
                85                  90                  95      


Ile Lys Ser Phe Pro Gly His Thr Ser Ser Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Phe Val Phe Gln 
        115                 120                 125             


Asn Arg Glu Glu Phe Tyr Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 


Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 


Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     


Asp Gly 
        


<210>  20
<211>  178
<212>  PRT
<213>  artificial

<220>
<223>  lipocalin mutein 

<400>  20

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Gln Ala Gly Asn Ile Arg Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              


Ser Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  


Asp Val Thr Ala Val Thr Phe Asp Asp Lys Lys Cys Asn Tyr Ala Ile 
65                  70                  75                  80  


Ser Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Lys 
                85                  90                  95      


Ile Lys Ser Phe Pro Gly His Thr Ser Ser Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Phe Val Phe Gln 
        115                 120                 125             


Asn Arg Glu Glu Phe Tyr Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 


Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 


Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     


Asp Gly 
        


<210>  21
<211>  10
<212>  PRT
<213>  artificial

<220>
<223>  C-terminal tag 1

<400>  21

Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
1               5                   10  


<210>  22
<211>  11
<212>  PRT
<213>  artificial

<220>
<223>  C-terminal tag 2

<400>  22

Pro Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
1               5                   10      


<210>  23
<211>  8
<212>  PRT
<213>  artificial

<220>
<223>  Strep-tag

<400>  23

Trp Ser His Pro Gln Phe Glu Lys 
1               5               


<210>  24
<211>  456
<212>  DNA
<213>  artificial

<220>
<223>  lipocalin mutein 

<400>  24
gcctcagacg aggagattca ggatgtgtca gggacgtggt atctgaaggc catgacggtg       60

gatgaggggt gtcgtccttg gaatatattt tcagttacgc caatgactct gactaccctt      120

gaaggcggca atctggaggc taaggtcacc atggcaatag atgggccggc acaggaggtg      180

aaagcagtgt tagagaagac agatgaaccg ggtaaatata cggccgatgg cggtaaacat      240

gttgcctata tcattcgcag ccatgtgaaa gatcattaca tcttttatag cgagggcgtg      300

tgcgatgggt ctcctgttcc aggggtgtgg ctcgtgggca gagaccccaa gaacaacctg      360

gaagccttgg aggactttga gaaagccgca ggagcccgcg gactcagcac ggagagcatc      420

ctcatcccca ggcagagcga aaccagctct ccaggg                                456


<210>  25
<211>  459
<212>  DNA
<213>  artificial

<220>
<223>  lipocalin mutein 

<400>  25
acctcagacg aggagattca ggatgtgtca gggacgtggt atctgaaggc gatgacggtg       60

gatgaggggt gtcgtccttg gaatatattt tcagttacgc caatgactct gactaccctt      120

gaaggcggca atctggaggc taaggtcacc atggcaatag atgggccggc acaggaggtg      180

agagcagtgt tagagaagac agatgaaccg ggtaaatata cggccgacgg cggtaaacat      240

gatgcctata tcattcgcag ccatgtgaaa gatcattaca tcttttatag cgagggcgtg      300

tgcgatgggt ctcctgttcc gggggtgtgg ctcgtgggca gagaccccga gaacaacctg      360

gaagccttgg aggactttga gaaaaccgca ggagcccgcg gactcagcac ggagagcatc      420

ctcatcccca ggcagagcga aaccagctct ccagggcca                             459


<210>  26
<211>  456
<212>  DNA
<213>  artificial

<220>
<223>  lipocalin mutein 

<400>  26
gcctcagacg aggagattca ggatgtgtca gggacgtggt atctgaaggc gatgacggtg       60

gatgaggggt gtcgtccttg gaatatattt tcagttacgc caatgactct gactaccctt      120

gaaggcggca atctggaggc taaggtcacc atggcaatag atgggccggc acaggaggtg      180

aacgcagtgt tagagaagac agatgaaccg ggtaaatata cggccgatgg cggtaaacat      240

gttgcctata tcattcgcag ccatgtgaga gatcattaca tcttttatag cgagggcgtg      300

tgcgatgggt ctcctgttcc gggggtgtgg ctcgtgggca gggaccccga gaacaacctg      360

gaagccttgg aggactttga gaaaaccgca ggagcccgcg gactcagcac ggagagcatt      420

ctcattccca ggcagagcga aaccagctct ccaggg                                456


<210>  27
<211>  459
<212>  DNA
<213>  artificial

<220>
<223>  lipocalin mutein 

<400>  27
gtctcagacg aggagattca ggatgtgtca gggacgtggt atctgaaggc gatgacggtg       60

gatgaggggt gtcgtccttg gaatatattt tcagttacgc caatgactct gactaccctt      120

gaaggcggca atctggaggc taaggtcacc atggcaatag atgggccggc acaggaggtg      180

agagcagtgt tagagaagac agatgaaccg ggtaaatata cggccgatgg cggtaaacat      240

gttgcctata tcattcgcag ccatgtggaa gatcattaca tcttttatag cgagggcgtg      300

tgcgatgggt ctcctgttcc gggggtgtgg ctcgtgggca gagaccccga gaacaacctg      360

gaagccttgg aggactttga gaaaaccgca ggagcccgcg gactcagcac ggagagcatc      420

ctcatcccca ggcagagcga aaccagctct ccagggcca                             459


<210>  28
<211>  459
<212>  DNA
<213>  artificial

<220>
<223>  lipocalin mutein 

<400>  28
gcctcagacg aggagattca ggatgtgtca gggacgtggt atctgaaggc gatgacggtg       60

gatgaggggt gtcgtccttg gaatatattt tcagttacgc caatgactct gtctaccctt      120

gaaggcggca atctggaggc taaggtcacc atggcaatag atgggccggc acaggaggtg      180

aaagcagtgt tagagaagac agatgaaccg ggtaaatata cggccgatgg cggtaaacat      240

gttgcctata tcattcgcag ccatgtgaaa gatcattaca tcttttatag cgagggcgtg      300

tgcgatgggt ctcctgttcc gggggtgtgg ctcgtgggca gagaccccaa gaacaacctg      360

gaagccttgg aggactttga gaaagccgca ggagcccgcg gactcagcac ggagagcatc      420

ctcatcccca ggcagatcga aaccagctct ccagggcca                             459


<210>  29
<211>  459
<212>  DNA
<213>  artificial

<220>
<223>  lipocalin mutein 

<400>  29
gcctcagacg aggagattca ggatgtgtca gggacgtggt atctgaaggc gatgacggtg       60

gatgaggggt gtcgtccttg gaatatattt tcagttacgc caatgactct gactaccctt      120

gaaggcggca atctggaggc tgaggtcacc atggcaatag atgggccggc acaggaggtg      180

aaagcagtgt tagagaaggc agatgaaccg ggtaaatata cggccgatgg cggtaaacat      240

gttgcctata tcattcgcag ccatgtgaaa gatcattaca tcttttatag cgagggcgtg      300

tgcgatgggt ctcctgttcc gggggtgtgg ctcgtgggca gagaccccaa gaacaacctg      360

gaagccttgg aggactttga gaaaaccgca ggagcccgcg gactcagcac ggagagcatc      420

ctcatcccca gtcagatcga aaccagctct ccagggcca                             459


<210>  30
<211>  459
<212>  DNA
<213>  artificial

<220>
<223>  lipocalin mutein 

<400>  30
acctcagacg aggagattca ggatgtgtca gggacgtggt atctgaaggc gatgacggtg       60

gatgaggggt gtcgtccttg gaatatattt tcagttacgc caatgactct gactaccctt      120

gaagacggca atctggaggc taaggtcacc atggcaatag atgggccggc acaggaggtg      180

aaagcagtgt tagagaaggc agatgaaccg ggtaaatata cggccgatgg cggtaaacat      240

gttgcctata tcattcgcag ccatgtgaaa gatcattaca tcttttatag cgagggcgtg      300

tgcgatgggt ctcctgttcc gggggtgtgg ctcgtgggca gagaccccaa gaacaacctg      360

gaagccttgg aggactttga gaaagccgca ggagcccgcg gactcagcac ggagagcatc      420

ctcatcccca ggcagatcga aaccagctct ccagggcca                             459


<210>  31
<211>  534
<212>  DNA
<213>  artificial

<220>
<223>  lipocalin mutein 

<400>  31
caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag       60

aacttccagg acaaccaatt ccatgggaag tggtatgtgg taggtcaggc agggaatatc      120

aaactcagag aagacaaaga cccgaacaag atgatggcca ccatctatga gctgaaagaa      180

gacaagagct acaatgtcac cggtgtcact tttgacgaca agaagtgtac ttacgctatc      240

tctacttttg ttccaggttc ccagccaggc gagttcacgc tgggcaaaat taagagtttc      300

cctggacata cgagttctct cgtccgagtg gtgagcacca actacaacca gcatgctatg      360

gtgttcttca agttcgtttt ccaaaacagg gaggaattct acatcaccct ctacgggaga      420

accaaggagc tgacttcgga actaaaggag aacttcatcc gcttctccaa atctctgggc      480

ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc            534


<210>  32
<211>  534
<212>  DNA
<213>  artificial

<220>
<223>  lipocalin mutein 

<400>  32
caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag       60

aacttccagg acaaccaatt ccatgggaaa tggtacgttg tcgggcaggc cggaaatatt      120

aggctgcgtg aggataagga tccgattaaa atgatggcga ccatttacga gttgaaagaa      180

gataaatcat atgacgtcac catggtgaag tttgatgata agaaatgcat gtacgatatt      240

tggacctttg tgccggggag ccagccgggc gagtttactt taggcaagat taaaagtttt      300

ccgggccata catcatcgtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg      360

gtgttcttca agtttgtgtt tcagaaccgc gaggagtttt atatcacact gtacgggcgc      420

acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc      480

ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc            534


<210>  33
<211>  534
<212>  DNA
<213>  artificial

<220>
<223>  lipocalin mutein 

<400>  33
caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag       60

aacttccagg acaaccaatt ccatgggaaa tggtacgttg tcgggcaggc cggaaatatt      120

aggctgcgtg aggataagga tccgaataaa atgatggcga ccatttacga gttgaaagaa      180

gataaatcat atgacgtcac cgcggtggcg tttgatgata agaaatgcac gtacgatatt      240

tggacctttg tgccggggag ccagccgggc gagtttactt taggcaagat taaaagtttt      300

ccgggccata catcatcgtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg      360

gtgttcttca agtttgtgtt tcagaaccgc gaggagtttt atatcacact gtacgggcgc      420

acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc      480

ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc            534


<210>  34
<211>  534
<212>  DNA
<213>  artificial

<220>
<223>  lipocalin mutein 

<400>  34
caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag       60

aacttccagg acaaccaatt ccatgggaaa tggtacgttg tcgggcaggc cggaaatatt      120

aagctgcgtg aggataagga tccgaataaa atgatggcga ccatttacga gttgaaagaa      180

gataaatcat atgacgtcac cgcggtggcg tttgatgata agaaatgcac gtacgatatt      240

tggacctttg tgccggggag ccagccgggc gagtttactt taggcaagat taaaagtttt      300

ccgggccata catcatcttt ggtccgcgtc gtgagcacca actacaacca gcatgccatg      360

gtgttcttca agtttgtgtt tcagaaccgc gaggagtttt atatcacact gtacgggcgc      420

acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc      480

ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc            534


<210>  35
<211>  525
<212>  DNA
<213>  artificial

<220>
<223>  lipocalin mutein 

<400>  35
caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag       60

aacttccagg acaaccaatt ccatgggaaa tggtacgttg tcgggcaggc cggaaatatt      120

aagctgcgtg aggatagtaa aatgatggcg accatttacg agttgaaaga agataaatca      180

tatgacgtca ccggtgtgag ttttgatgat aagaaatgca cgtacgctat tatgaccttt      240

gtgccgggga gccagccggg cgagtttact ttaggcaaga ttaaaagttt tccgggccat      300

acatcatcgt tggtccgcgt cgtgagcacc aactacaacc agcatgccat ggtgttcttc      360

aagtttgtgt ttcagaaccg cgaggagttt tatatcacac tgtacgggcg cacgaaagaa      420

ctgacaagcg agctgaagga aaattttatc cgcttttcca aatctctggg cctccctgaa      480

aaccacatcg tcttccctgt cccaatcgac cagtgtatcg acggc                      525


<210>  36
<211>  534
<212>  DNA
<213>  artificial

<220>
<223>  lipocalin mutein 

<400>  36
caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag       60

aacttccagg acaaccaatt ccatgggaaa tggtacgttg tcgggcaggc cggaaatatt      120

aagctgcgtg aggataagga tccggttaaa atgatggcga ccatttacga gttgaaagaa      180

gataaatcat atgacgtcac cggggtgacg tttgatgata agaaatgcag gtacgatatt      240

tcgacctttg tgccggggag ccagccgggc gagtttactt ttggcaagat taaaagtttt      300

ccgggccata catcatcgtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg      360

gtgttcttca agtttgtgtt tcagaaccgc gaggagtttt atatcacact gtacgggcgc      420

acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc      480

ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc            534


<210>  37
<211>  534
<212>  DNA
<213>  artificial

<220>
<223>  lipocalin mutein 

<400>  37
caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag       60

aacttccagg acaaccaatt ccatgggaaa tggtacgttg tcgggcaggc cggaaatatt      120

aggctgcgtg aggataagga tccgcataaa atgatggcga ccatttacga gttgaaagaa      180

gataaatcat atgacgtcac cggggtgact tttgatgata agaaatgcac gtacgctatt      240

tcgacctttg tgccggggag ccagccgggc gagtttactt taggcaagat taaaagtttt      300

ccgggccata catcatcttt ggtccgcgtc gtgagcacca actacaacca gcatgccatg      360

gtgttcttca agtttgtgtt tcagaaccgc gaggagtttt atatcacact gtacgggcgc      420

acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc      480

ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc            534


<210>  38
<211>  534
<212>  DNA
<213>  artificial

<220>
<223>  lipocalin mutein 

<400>  38
caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag       60

aacttccagg acaaccaatt ccatgggaaa tggtacgttg tcgggcaggc cggaaatatt      120

aagctgcgtg aggataagga tccgaataaa atgatggcga ccatttacga gttgaaagaa      180

gataaatcat atgacgtcac cggggtgact tttgatgata agaaatgcac gtacgctatt      240

tctacccttg tgccggggag ccagccgggc gagtttactt ttggcaagat taaaagtttt      300

ccgggccata catcatcgtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg      360

gtgttcttca agtttgtgtt tcagaaccgc gaggagtttt atatcacact gtacgggcgc      420

acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc      480

ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc            534


<210>  39
<211>  534
<212>  DNA
<213>  artificial

<220>
<223>  lipocalin mutein 

<400>  39
caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag       60

aacttccagg acaaccaatt ccatgggaaa tggtacgttg tcgggcaggc cggaaatatt      120

aggctgcgtg aggataagga tccgtctaaa atgatggcga ccatttacga gttgaaagaa      180

gataaatcat atgacgtcac cgctgtgacg tttgatgata agaaatgcaa ttacgctatt      240

tctacctttg tgccggggag ccagccgggc gagtttactt taggcaagat taaaagtttt      300

ccgggccata catcatcgtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg      360

gtgttcttca agtttgtgtt tcagaaccgc gaggagtttt atatcacact gtacgggcgc      420

acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc      480

ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc            534


