                         SEQUENCE LISTING

<110>  Evonik Industries AG
 
<120>  A METHOD FOR PRODUCING ACYL AMINO ACIDS

<130>  201500025

<150>  15166355.6
<151>  2015-05-05

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  294
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Leu Val Leu His Asn Ser Gln Lys Leu Gln Ile Leu Tyr Lys Ser 
1               5                   10                  15      


Leu Glu Lys Ser Ile Pro Glu Ser Ile Lys Val Tyr Gly Ala Ile Phe 
            20                  25                  30          


Asn Ile Lys Asp Lys Asn Pro Phe Asn Met Glu Val Leu Val Asp Ala 
        35                  40                  45              


Trp Pro Asp Tyr Gln Ile Val Ile Thr Arg Pro Gln Lys Gln Glu Met 
    50                  55                  60                  


Lys Asp Asp Gln Asp His Tyr Thr Asn Thr Tyr His Ile Phe Thr Lys 
65                  70                  75                  80  


Ala Pro Asp Lys Leu Glu Glu Val Leu Ser Tyr Ser Asn Val Ile Ser 
                85                  90                  95      


Trp Glu Gln Thr Leu Gln Ile Gln Gly Cys Gln Glu Gly Leu Asp Glu 
            100                 105                 110         


Ala Ile Arg Lys Val Ala Thr Ser Lys Ser Val Gln Val Asp Tyr Met 
        115                 120                 125             


Lys Thr Ile Leu Phe Ile Pro Glu Leu Pro Lys Lys His Lys Thr Ser 
    130                 135                 140                 


Ser Asn Asp Lys Met Glu Leu Phe Glu Val Asp Asp Asp Asn Lys Glu 
145                 150                 155                 160 


Gly Asn Phe Ser Asn Met Phe Leu Asp Ala Ser His Ala Gly Leu Val 
                165                 170                 175     


Asn Glu His Trp Ala Phe Gly Lys Asn Glu Arg Ser Leu Lys Tyr Ile 
            180                 185                 190         


Glu Arg Cys Leu Gln Asp Phe Leu Gly Phe Gly Val Leu Gly Pro Glu 
        195                 200                 205             


Gly Gln Leu Val Ser Trp Ile Val Met Glu Gln Ser Cys Glu Leu Arg 
    210                 215                 220                 


Met Gly Tyr Thr Val Pro Lys Tyr Arg His Gln Gly Asn Met Leu Gln 
225                 230                 235                 240 


Ile Gly Tyr His Leu Glu Lys Tyr Leu Ser Gln Lys Glu Ile Pro Phe 
                245                 250                 255     


Tyr Phe His Val Ala Asp Asn Asn Glu Lys Ser Leu Gln Ala Leu Asn 
            260                 265                 270         


Asn Leu Gly Phe Lys Ile Cys Pro Cys Gly Trp His Gln Trp Lys Cys 
        275                 280                 285             


Thr Pro Lys Lys Tyr Cys 
    290                 


<210>  2
<211>  561
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FadD Acyl-CoA synthase

<400>  2

Met Lys Lys Val Trp Leu Asn Arg Tyr Pro Ala Asp Val Pro Thr Glu 
1               5                   10                  15      


Ile Asn Pro Asp Arg Tyr Gln Ser Leu Val Asp Met Phe Glu Gln Ser 
            20                  25                  30          


Val Ala Arg Tyr Ala Asp Gln Pro Ala Phe Val Asn Met Gly Glu Val 
        35                  40                  45              


Met Thr Phe Arg Lys Leu Glu Glu Arg Ser Arg Ala Phe Ala Ala Tyr 
    50                  55                  60                  


Leu Gln Gln Gly Leu Gly Leu Lys Lys Gly Asp Arg Val Ala Leu Met 
65                  70                  75                  80  


Met Pro Asn Leu Leu Gln Tyr Pro Val Ala Leu Phe Gly Ile Leu Arg 
                85                  90                  95      


Ala Gly Met Ile Val Val Asn Val Asn Pro Leu Tyr Thr Pro Arg Glu 
            100                 105                 110         


Leu Glu His Gln Leu Asn Asp Ser Gly Ala Ser Ala Ile Val Ile Val 
        115                 120                 125             


Ser Asn Phe Ala His Thr Leu Glu Lys Val Val Asp Lys Thr Ala Val 
    130                 135                 140                 


Gln His Val Ile Leu Thr Arg Met Gly Asp Gln Leu Ser Thr Ala Lys 
145                 150                 155                 160 


Gly Thr Val Val Asn Phe Val Val Lys Tyr Ile Lys Arg Leu Val Pro 
                165                 170                 175     


Lys Tyr His Leu Pro Asp Ala Ile Ser Phe Arg Ser Ala Leu His Asn 
            180                 185                 190         


Gly Tyr Arg Met Gln Tyr Val Lys Pro Glu Leu Val Pro Glu Asp Leu 
        195                 200                 205             


Ala Phe Leu Gln Tyr Thr Gly Gly Thr Thr Gly Val Ala Lys Gly Ala 
    210                 215                 220                 


Met Leu Thr His Arg Asn Met Leu Ala Asn Leu Glu Gln Val Asn Ala 
225                 230                 235                 240 


Thr Tyr Gly Pro Leu Leu His Pro Gly Lys Glu Leu Val Val Thr Ala 
                245                 250                 255     


Leu Pro Leu Tyr His Ile Phe Ala Leu Thr Ile Asn Cys Leu Leu Phe 
            260                 265                 270         


Ile Glu Leu Gly Gly Gln Asn Leu Leu Ile Thr Asn Pro Arg Asp Ile 
        275                 280                 285             


Pro Gly Leu Val Lys Glu Leu Ala Lys Tyr Pro Phe Thr Ala Ile Thr 
    290                 295                 300                 


Gly Val Asn Thr Leu Phe Asn Ala Leu Leu Asn Asn Lys Glu Phe Gln 
305                 310                 315                 320 


Gln Leu Asp Phe Ser Ser Leu His Leu Ser Ala Gly Gly Gly Met Pro 
                325                 330                 335     


Val Gln Gln Val Val Ala Glu Arg Trp Val Lys Leu Thr Gly Gln Tyr 
            340                 345                 350         


Leu Leu Glu Gly Tyr Gly Leu Thr Glu Cys Ala Pro Leu Val Ser Val 
        355                 360                 365             


Asn Pro Tyr Asp Ile Asp Tyr His Ser Gly Ser Ile Gly Leu Pro Val 
    370                 375                 380                 


Pro Ser Thr Glu Ala Lys Leu Val Asp Asp Asp Asp Asn Glu Val Pro 
385                 390                 395                 400 


Pro Gly Gln Pro Gly Glu Leu Cys Val Lys Gly Pro Gln Val Met Leu 
                405                 410                 415     


Gly Tyr Trp Gln Arg Pro Asp Ala Thr Asp Glu Ile Ile Lys Asn Gly 
            420                 425                 430         


Trp Leu His Thr Gly Asp Ile Ala Val Met Asp Glu Glu Gly Phe Leu 
        435                 440                 445             


Arg Ile Val Asp Arg Lys Lys Asp Met Ile Leu Val Ser Gly Phe Asn 
    450                 455                 460                 


Val Tyr Pro Asn Glu Ile Glu Asp Val Val Met Gln His Pro Gly Val 
465                 470                 475                 480 


Gln Glu Val Ala Ala Val Gly Val Pro Ser Gly Ser Ser Gly Glu Ala 
                485                 490                 495     


Val Lys Ile Phe Val Val Lys Lys Asp Pro Ser Leu Thr Glu Glu Ser 
            500                 505                 510         


Leu Val Thr Phe Cys Arg Arg Gln Leu Thr Gly Tyr Lys Val Pro Lys 
        515                 520                 525             


Leu Val Glu Phe Arg Asp Glu Leu Pro Lys Ser Asn Val Gly Lys Ile 
    530                 535                 540                 


Leu Arg Arg Glu Leu Arg Asp Glu Ala Arg Gly Lys Val Asp Asn Lys 
545                 550                 555                 560 


Ala 
    


<210>  3
<211>  906
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synUcTE (an acyl-CoA thioesterase) gene (codon-optimized)

<400>  3
atgactctag agtggaaacc gaaaccaaaa ctgcctcaac tgctggatga tcacttcggt       60

ctgcacggtc tggtgtttcg tcgtactttc gcaattcgtt cttatgaagt gggtccagat      120

cgttctacct ccatcctggc cgtcatgaac cacatgcagg aagccaccct gaatcacgcg      180

aaatctgttg gtatcctggg tgatggtttc ggcactactc tggaaatgtc taaacgtgac      240

ctgatgtggg tagtgcgtcg cacccacgta gcagtagagc gctaccctac ttggggtgac      300

actgtggaag tcgagtgttg gattggcgcg tccggtaaca atggtatgcg tcgcgatttt      360

ctggtccgtg actgtaaaac gggcgaaatc ctgacgcgtt gcacctccct gagcgttctg      420

atgaacaccc gcactcgtcg cctgtctacc atcccggacg aagtgcgcgg tgagatcggt      480

cctgctttca tcgataacgt ggcagttaaa gacgacgaaa tcaagaaact gcaaaaactg      540

aacgactcca ccgcggacta catccagggc ggtctgactc cgcgctggaa cgacctggat      600

gttaatcagc atgtgaacaa cctgaaatac gttgcttggg tcttcgagac tgtgccggac      660

agcattttcg aaagccatca catttcctct tttactctgg agtaccgtcg cgaatgtact      720

cgcgactccg ttctgcgcag cctgaccacc gtaagcggcg gttctagcga ggcaggtctg      780

gtctgcgacc atctgctgca actggaaggc ggctccgaag tcctgcgtgc gcgtacggag      840

tggcgtccaa agctgacgga ttctttccgc ggcatctccg taattccggc ggaacctcgt      900

gtttaa                                                                 906


<210>  4
<211>  290
<212>  PRT
<213>  Thermomyces lanuginosus

<400>  4

Met Arg Ser Ser Leu Val Leu Phe Phe Val Ser Ala Trp Thr Ala Leu 
1               5                   10                  15      


Ala Ser Pro Ile Arg Arg Glu Val Ser Gln Asp Leu Phe Asn Gln Phe 
            20                  25                  30          


Asn Leu Phe Ala Gln Tyr Ser Ala Ala Ala Tyr Cys Gly Lys Asn Asn 
        35                  40                  45              


Asp Ala Pro Ala Gly Thr Asn Ile Thr Cys Thr Gly Asn Ala Cys Pro 
    50                  55                  60                  


Glu Val Glu Lys Ala Asp Ala Thr Phe Leu Tyr Ser Phe Glu Asp Ser 
65                  70                  75                  80  


Gly Val Gly Asp Val Thr Gly Phe Leu Ala Leu Asp Asn Thr Asn Lys 
                85                  90                  95      


Leu Ile Val Leu Ser Phe Arg Gly Ser Arg Ser Ile Glu Asn Trp Ile 
            100                 105                 110         


Gly Asn Leu Asn Phe Asp Leu Lys Glu Ile Asn Asp Ile Cys Ser Gly 
        115                 120                 125             


Cys Arg Gly His Asp Gly Phe Thr Ser Ser Trp Arg Ser Val Ala Asp 
    130                 135                 140                 


Thr Leu Arg Gln Lys Val Glu Asp Ala Val Arg Glu His Pro Asp Tyr 
145                 150                 155                 160 


Arg Val Val Phe Thr Gly His Ser Leu Gly Gly Ala Leu Ala Thr Val 
                165                 170                 175     


Ala Gly Ala Asp Leu Arg Gly Asn Gly Tyr Asp Ile Asp Val Phe Ser 
            180                 185                 190         


Tyr Gly Ala Pro Arg Val Gly Asn Arg Ala Phe Ala Glu Phe Leu Thr 
        195                 200                 205             


Val Gln Thr Gly Gly Thr Leu Tyr Arg Ile Thr His Thr Asn Asp Ile 
    210                 215                 220                 


Val Pro Arg Leu Pro Pro Arg Glu Phe Gly Tyr Ser His Ser Ser Pro 
225                 230                 235                 240 


Glu Tyr Trp Ile Lys Ser Gly Thr Leu Val Val Thr Arg Asn Asp Ile 
                245                 250                 255     


Val Lys Ile Glu Gly Ile Asp Ala Thr Gly Gly Asn Asn Gln Pro Asn 
            260                 265                 270         


Ile Pro Asp Ile Pro Ala His Leu Trp Tyr Phe Gly Leu Ile Gly Thr 
        275                 280                 285             


Cys Leu 
    290 


<210>  5
<211>  230
<212>  PRT
<213>  Pseudomonas putida

<400>  5

Met Ser Phe Ser Asn Tyr Lys Val Ile Ala Met Pro Val Leu Val Ala 
1               5                   10                  15      


Asn Phe Val Leu Gly Ala Ala Thr Ala Trp Ala Asn Glu Asn Tyr Pro 
            20                  25                  30          


Ala Lys Ser Ala Gly Tyr Asn Gln Gly Asp Trp Val Ala Ser Phe Asn 
        35                  40                  45              


Phe Ser Lys Val Tyr Val Gly Glu Glu Leu Gly Asp Leu Asn Val Gly 
    50                  55                  60                  


Gly Gly Ala Leu Pro Asn Ala Asp Val Ser Ile Gly Asn Asp Thr Thr 
65                  70                  75                  80  


Leu Thr Phe Asp Ile Ala Tyr Phe Val Ser Ser Asn Ile Ala Val Asp 
                85                  90                  95      


Phe Phe Val Gly Val Pro Ala Arg Ala Lys Phe Gln Gly Glu Lys Ser 
            100                 105                 110         


Ile Ser Ser Leu Gly Arg Val Ser Glu Val Asp Tyr Gly Pro Ala Ile 
        115                 120                 125             


Leu Ser Leu Gln Tyr His Tyr Asp Ser Phe Glu Arg Leu Tyr Pro Tyr 
    130                 135                 140                 


Val Gly Val Gly Val Gly Arg Val Leu Phe Phe Asp Lys Thr Asp Gly 
145                 150                 155                 160 


Ala Leu Ser Ser Phe Asp Ile Lys Asp Lys Trp Ala Pro Ala Phe Gln 
                165                 170                 175     


Val Gly Leu Arg Tyr Asp Leu Gly Asn Ser Trp Met Leu Asn Ser Asp 
            180                 185                 190         


Val Arg Tyr Ile Pro Phe Lys Thr Asp Val Thr Gly Thr Leu Gly Pro 
        195                 200                 205             


Val Pro Val Ser Thr Lys Ile Glu Val Asp Pro Phe Ile Leu Ser Leu 
    210                 215                 220                 


Gly Ala Ser Tyr Val Phe 
225                 230 


<210>  6
<211>  448
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FadL

<400>  6

Met Val Met Ser Gln Lys Thr Leu Phe Thr Lys Ser Ala Leu Ala Val 
1               5                   10                  15      


Ala Val Ala Leu Ile Ser Thr Gln Ala Trp Ser Ala Gly Phe Gln Leu 
            20                  25                  30          


Asn Glu Phe Ser Ser Ser Gly Leu Gly Arg Ala Tyr Ser Gly Glu Gly 
        35                  40                  45              


Ala Ile Ala Asp Asp Ala Gly Asn Val Ser Arg Asn Pro Ala Leu Ile 
    50                  55                  60                  


Thr Met Phe Asp Arg Pro Thr Phe Ser Ala Gly Ala Val Tyr Ile Asp 
65                  70                  75                  80  


Pro Asp Val Asn Ile Ser Gly Thr Ser Pro Ser Gly Arg Ser Leu Lys 
                85                  90                  95      


Ala Asp Asn Ile Ala Pro Thr Ala Trp Val Pro Asn Met His Phe Val 
            100                 105                 110         


Ala Pro Ile Asn Asp Gln Phe Gly Trp Gly Ala Ser Ile Thr Ser Asn 
        115                 120                 125             


Tyr Gly Leu Ala Thr Glu Phe Asn Asp Thr Tyr Ala Gly Gly Ser Val 
    130                 135                 140                 


Gly Gly Thr Thr Asp Leu Glu Thr Met Asn Leu Asn Leu Ser Gly Ala 
145                 150                 155                 160 


Tyr Arg Leu Asn Asn Ala Trp Ser Phe Gly Leu Gly Phe Asn Ala Val 
                165                 170                 175     


Tyr Ala Arg Ala Lys Ile Glu Arg Phe Ala Gly Asp Leu Gly Gln Leu 
            180                 185                 190         


Val Ala Gly Gln Ile Met Gln Ser Pro Ala Gly Gln Thr Gln Gln Gly 
        195                 200                 205             


Gln Ala Leu Ala Ala Thr Ala Asn Gly Ile Asp Ser Asn Thr Lys Ile 
    210                 215                 220                 


Ala His Leu Asn Gly Asn Gln Trp Gly Phe Gly Trp Asn Ala Gly Ile 
225                 230                 235                 240 


Leu Tyr Glu Leu Asp Lys Asn Asn Arg Tyr Ala Leu Thr Tyr Arg Ser 
                245                 250                 255     


Glu Val Lys Ile Asp Phe Lys Gly Asn Tyr Ser Ser Asp Leu Asn Arg 
            260                 265                 270         


Ala Phe Asn Asn Tyr Gly Leu Pro Ile Pro Thr Ala Thr Gly Gly Ala 
        275                 280                 285             


Thr Gln Ser Gly Tyr Leu Thr Leu Asn Leu Pro Glu Met Trp Glu Val 
    290                 295                 300                 


Ser Gly Tyr Asn Arg Val Asp Pro Gln Trp Ala Ile His Tyr Ser Leu 
305                 310                 315                 320 


Ala Tyr Thr Ser Trp Ser Gln Phe Gln Gln Leu Lys Ala Thr Ser Thr 
                325                 330                 335     


Ser Gly Asp Thr Leu Phe Gln Lys His Glu Gly Phe Lys Asp Ala Tyr 
            340                 345                 350         


Arg Ile Ala Leu Gly Thr Thr Tyr Tyr Tyr Asp Asp Asn Trp Thr Phe 
        355                 360                 365             


Arg Thr Gly Ile Ala Phe Asp Asp Ser Pro Val Pro Ala Gln Asn Arg 
    370                 375                 380                 


Ser Ile Ser Ile Pro Asp Gln Asp Arg Phe Trp Leu Ser Ala Gly Thr 
385                 390                 395                 400 


Thr Tyr Ala Phe Asn Lys Asp Ala Ser Val Asp Val Gly Val Ser Tyr 
                405                 410                 415     


Met His Gly Gln Ser Val Lys Ile Asn Glu Gly Pro Tyr Gln Phe Glu 
            420                 425                 430         


Ser Glu Gly Lys Ala Trp Leu Phe Gly Thr Asn Phe Asn Tyr Ala Phe 
        435                 440                 445             


<210>  7
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ompAfwd

<400>  7
cggataacaa tttcacacag aggagaaatt aactatgaaa aaaac                       45


<210>  8
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LipT1-rev

<400>  8
gggtaccgag ctcgcatgcg tcacaggcag gtgccaatca                             40


<210>  9
<211>  4541
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  vector pQE80L-kan

<400>  9
ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca       60

attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga      120

ggatcgcatc accatcacca tcacggatcc gcatgcgagc tcggtacccc gggtcgacct      180

gcagccaagc ttaattagct gagcttggac tcctgttgat agatccagta atgacctcag      240

aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt attggtgaga      300

atccaagcta gcttggcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc      360

actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt      420

cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta      480

aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc      540

ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag acggtgagct ggtgatatgg      600

gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc      660

tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg      720

tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc      780

tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac      840

ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg      900

ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt      960

aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt     1020

tattggtgcc cttaaacgcc tggggtaatg actctctagc ttgaggcatc aaataaaacg     1080

aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct     1140

cctgagtagg acaaatccgc cctctagatt acgtgcagtc gatgataagc tgtcaaacat     1200

gagaattgtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct     1260

ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga     1320

ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag     1380

ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg     1440

ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc     1500

ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc cggactcggt     1560

aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac     1620

gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc     1680

ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag     1740

acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc     1800

caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact     1860

gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc     1920

ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg     1980

ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat     2040

cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg     2100

cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc     2160

cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac     2220

tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg     2280

ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac     2340

cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcaccattc     2400

gatggtgtcg gaatttcggg cagcgttggg tcctggccac gggtgcgcat gatctagagc     2460

tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg     2520

gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg     2580

ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat     2640

actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg     2700

aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc     2760

tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg     2820

cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag     2880

gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc     2940

gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag     3000

gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga     3060

ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc     3120

atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg     3180

tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt     3240

ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca     3300

gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca     3360

ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag     3420

ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca     3480

agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg     3540

ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg gcgttaaggg     3600

attttggtca tgaattaatt cttagaaaaa ctcatcgagc atcaaatgaa actgcaattt     3660

attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga     3720

aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac     3780

tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga     3840

gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagtttat gcatttcttt     3900

ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa     3960

accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg     4020

acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat     4080

attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc     4140

agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg     4200

cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct     4260

acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat     4320

tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc     4380

catgttggaa tttaatcgcg gcctagagca agacgtttcc cgttgaatat ggctcataac     4440

accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgacc atgacattaa     4500

cctataaaaa taggcgtatc acgaggccct ttcgtcttca c                         4541


<210>  10
<211>  999
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  amplified PCR product

<400>  10
cggataacaa tttcacacag aggagaaatt aactatgaaa aaaactgcta tcgctatcgc       60

tgttgctctg gctggtttcg ctactgttgc tcaggccgaa ttcatgcgca gcagcctggt      120

gctgtttttt gtgagcgcgt ggaccgcgct ggcgagcccg attcgccgcg aagtgagcca      180

ggatctgttt aaccagttta acctgtttgc gcagtatagc gcggcggcgt attgcggcaa      240

aaacaacgat gcgccggcgg gcaccaacat tacctgcacc ggcaacgcgt gcccggaagt      300

ggaaaaagcg gatgcgacct ttctgtatag ctttgaagat agcggcgtgg gcgatgtgac      360

cggctttctg gcgctggata acaccaacaa actgattgtg ctgagctttc gcggcagccg      420

cagcattgaa aactggattg gcaacctgaa ctttgatctg aaagaaatta acgatatttg      480

cagcggctgc cgcggccatg atggctttac cagcagctgg cgcagcgtgg cggataccct      540

gcgccagaaa gtggaagatg cggtgcgcga acatccggat tatcgcgtgg tgtttaccgg      600

ccatagcctg ggcggcgcgc tggcgaccgt ggcgggcgcg gatctgcgcg gcaacggcta      660

tgatattgat gtgtttagct atggcgcgcc gcgcgtgggc aaccgcgcgt ttgcggaatt      720

tctgaccgtg cagaccggcg gcaccctgta tcgcattacc cataccaacg atattgtgcc      780

gcgcctgccg ccgcgcgaat ttggctatag ccatagcagc ccggaatatt ggattaaaag      840

cggcaccctg gtgccggtga cccgcaacga tattgtgaaa attgaaggca ttgatgcgac      900

cggcggcaac aaccagccga acattccgga tattccggcg catctgtggt attttggcct      960

gattggcacc tgcctgtgac gcatgcgagc tcggtaccc                             999


<210>  11
<211>  5439
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pQE80L-kan{PT5}[ompA-lipTl(co_Ec)]

<400>  11
cgggtcgacc tgcagccaag cttaattagc tgagcttgga ctcctgttga tagatccagt       60

aatgacctca gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt      120

tattggtgag aatccaagct agcttggcga gattttcagg agctaaggaa gctaaaatgg      180

agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt      240

ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta      300

cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca      360

ttcttgcccg cctgatgaat gctcatccgg aatttcgtat ggcaatgaaa gacggtgagc      420

tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt      480

tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc      540

aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata      600

tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca      660

atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca      720

aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg      780

gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttt      840

tttaaggcag ttattggtgc ccttaaacgc ctggggtaat gactctctag cttgaggcat      900

caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg      960

gtgaacgctc tcctgagtag gacaaatccg ccctctagat tacgtgcagt cgatgataag     1020

ctgtcaaaca tgagaattgt gcctaatgag tgagctaact tacattaatt gcgttgcgct     1080

cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac     1140

gcgcggggag aggcggtttg cgtattgggc gccagggtgg tttttctttt caccagtgag     1200

acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc     1260

acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa     1320

catgagctgt cttcggtatc gtcgtatccc actaccgaga tatccgcacc aacgcgcagc     1380

ccggactcgg taatggcgcg cattgcgccc agcgccatct gatcgttggc aaccagcatc     1440

gcagtgggaa cgatgccctc attcagcatt tgcatggttt gttgaaaacc ggacatggca     1500

ctccagtcgc cttcccgttc cgctatcggc tgaatttgat tgcgagtgag atatttatgc     1560

cagccagcca gacgcagacg cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt     1620

tgctggtgac ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag     1680

aaaataatac tgttgatggg tgtctggtca gagacatcaa gaaataacgc cggaacatta     1740

gtgcaggcag cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc     1800

ccactgacgc gttgcgcgag aagattgtgc accgccgctt tacaggcttc gacgccgctt     1860

cgttctacca tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc     1920

gcgacaattt gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac     1980

gactgtttgc ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc     2040

gccgcttcca ctttttcccg cgttttcgca gaaacgtggc tggcctggtt caccacgcgg     2100

gaaacggtct gataagagac accggcatac tctgcgacat cgtataacgt tactggtttc     2160

acattcacca ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt     2220

ttgcaccatt cgatggtgtc ggaatttcgg gcagcgttgg gtcctggcca cgggtgcgca     2280

tgatctagag ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc     2340

tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg     2400

gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata     2460

gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca     2520

tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc     2580

cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc     2640

tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat     2700

gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt     2760

ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg     2820

aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc     2880

tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt     2940

ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa     3000

gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta     3060

tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa     3120

caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa     3180

ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt     3240

cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt     3300

ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat     3360

cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat     3420

ggcgttaagg gattttggtc atgaattaat tcttagaaaa actcatcgag catcaaatga     3480

aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt     3540

aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct     3600

gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg     3660

ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagttta     3720

tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc     3780

gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg     3840

ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc     3900

gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc     3960

ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg     4020

gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca     4080

ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac     4140

aatcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat     4200

aaatcagcat ccatgttgga atttaatcgc ggcctagagc aagacgtttc ccgttgaata     4260

tggctcataa caccccttgt attactgttt atgtaagcag acagttttat tgttcatgac     4320

catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc acctcgagaa     4380

atcataaaaa atttatttgc tttgtgagcg gataacaatt ataatagatt caattgtgag     4440

cggataacaa tttcacacag aggagaaatt aactatgaaa aaaactgcta tcgctatcgc     4500

tgttgctctg gctggtttcg ctactgttgc tcaggccgaa ttcatgcgca gcagcctggt     4560

gctgtttttt gtgagcgcgt ggaccgcgct ggcgagcccg attcgccgcg aagtgagcca     4620

ggatctgttt aaccagttta acctgtttgc gcagtatagc gcggcggcgt attgcggcaa     4680

aaacaacgat gcgccggcgg gcaccaacat tacctgcacc ggcaacgcgt gcccggaagt     4740

ggaaaaagcg gatgcgacct ttctgtatag ctttgaagat agcggcgtgg gcgatgtgac     4800

cggctttctg gcgctggata acaccaacaa actgattgtg ctgagctttc gcggcagccg     4860

cagcattgaa aactggattg gcaacctgaa ctttgatctg aaagaaatta acgatatttg     4920

cagcggctgc cgcggccatg atggctttac cagcagctgg cgcagcgtgg cggataccct     4980

gcgccagaaa gtggaagatg cggtgcgcga acatccggat tatcgcgtgg tgtttaccgg     5040

ccatagcctg ggcggcgcgc tggcgaccgt ggcgggcgcg gatctgcgcg gcaacggcta     5100

tgatattgat gtgtttagct atggcgcgcc gcgcgtgggc aaccgcgcgt ttgcggaatt     5160

tctgaccgtg cagaccggcg gcaccctgta tcgcattacc cataccaacg atattgtgcc     5220

gcgcctgccg ccgcgcgaat ttggctatag ccatagcagc ccggaatatt ggattaaaag     5280

cggcaccctg gtgccggtga cccgcaacga tattgtgaaa attgaaggca ttgatgcgac     5340

cggcggcaac aaccagccga acattccgga tattccggcg catctgtggt attttggcct     5400

gattggcacc tgcctgtgac gcatgcgagc tcggtaccc                            5439


<210>  12
<211>  314
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  the fusion-protein ompA-LipTl

<400>  12

Met Lys Lys Thr Ala Ile Ala Ile Ala Val Ala Leu Ala Gly Phe Ala 
1               5                   10                  15      


Thr Val Ala Gln Ala Glu Phe Met Arg Ser Ser Leu Val Leu Phe Phe 
            20                  25                  30          


Val Ser Ala Trp Thr Ala Leu Ala Ser Pro Ile Arg Arg Glu Val Ser 
        35                  40                  45              


Gln Asp Leu Phe Asn Gln Phe Asn Leu Phe Ala Gln Tyr Ser Ala Ala 
    50                  55                  60                  


Ala Tyr Cys Gly Lys Asn Asn Asp Ala Pro Ala Gly Thr Asn Ile Thr 
65                  70                  75                  80  


Cys Thr Gly Asn Ala Cys Pro Glu Val Glu Lys Ala Asp Ala Thr Phe 
                85                  90                  95      


Leu Tyr Ser Phe Glu Asp Ser Gly Val Gly Asp Val Thr Gly Phe Leu 
            100                 105                 110         


Ala Leu Asp Asn Thr Asn Lys Leu Ile Val Leu Ser Phe Arg Gly Ser 
        115                 120                 125             


Arg Ser Ile Glu Asn Trp Ile Gly Asn Leu Asn Phe Asp Leu Lys Glu 
    130                 135                 140                 


Ile Asn Asp Ile Cys Ser Gly Cys Arg Gly His Asp Gly Phe Thr Ser 
145                 150                 155                 160 


Ser Trp Arg Ser Val Ala Asp Thr Leu Arg Gln Lys Val Glu Asp Ala 
                165                 170                 175     


Val Arg Glu His Pro Asp Tyr Arg Val Val Phe Thr Gly His Ser Leu 
            180                 185                 190         


Gly Gly Ala Leu Ala Thr Val Ala Gly Ala Asp Leu Arg Gly Asn Gly 
        195                 200                 205             


Tyr Asp Ile Asp Val Phe Ser Tyr Gly Ala Pro Arg Val Gly Asn Arg 
    210                 215                 220                 


Ala Phe Ala Glu Phe Leu Thr Val Gln Thr Gly Gly Thr Leu Tyr Arg 
225                 230                 235                 240 


Ile Thr His Thr Asn Asp Ile Val Pro Arg Leu Pro Pro Arg Glu Phe 
                245                 250                 255     


Gly Tyr Ser His Ser Ser Pro Glu Tyr Trp Ile Lys Ser Gly Thr Leu 
            260                 265                 270         


Val Pro Val Thr Arg Asn Asp Ile Val Lys Ile Glu Gly Ile Asp Ala 
        275                 280                 285             


Thr Gly Gly Asn Asn Gln Pro Asn Ile Pro Asp Ile Pro Ala His Leu 
    290                 295                 300                 


Trp Tyr Phe Gly Leu Ile Gly Thr Cys Leu 
305                 310                 


<210>  13
<211>  230
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AlkL mod1

<400>  13

Val Ser Phe Ser Asn Tyr Lys Val Ile Ala Met Pro Val Leu Val Ala 
1               5                   10                  15      


Asn Phe Val Leu Gly Ala Ala Thr Ala Trp Ala Asn Glu Asn Tyr Pro 
            20                  25                  30          


Ala Lys Ser Ala Gly Tyr Asn Gln Gly Asp Trp Val Ala Ser Phe Asn 
        35                  40                  45              


Phe Ser Lys Val Tyr Val Gly Glu Glu Leu Gly Asp Leu Asn Val Gly 
    50                  55                  60                  


Gly Gly Ala Leu Pro Asn Ala Asp Val Ser Ile Gly Asn Asp Thr Thr 
65                  70                  75                  80  


Leu Thr Phe Asp Ile Ala Tyr Phe Val Ser Ser Asn Ile Ala Val Asp 
                85                  90                  95      


Phe Phe Val Gly Val Pro Ala Arg Ala Lys Phe Gln Gly Glu Lys Ser 
            100                 105                 110         


Ile Ser Ser Leu Gly Arg Val Ser Glu Val Asp Tyr Gly Pro Ala Ile 
        115                 120                 125             


Leu Ser Leu Gln Tyr His Tyr Asp Ser Phe Glu Arg Leu Tyr Pro Tyr 
    130                 135                 140                 


Val Gly Val Gly Val Gly Arg Val Leu Phe Phe Asp Lys Thr Asp Gly 
145                 150                 155                 160 


Ala Leu Ser Ser Phe Asp Ile Lys Asp Lys Trp Ala Pro Ala Phe Gln 
                165                 170                 175     


Val Gly Leu Arg Tyr Asp Leu Gly Asn Ser Trp Met Leu Asn Ser Asp 
            180                 185                 190         


Val Arg Tyr Ile Pro Phe Lys Thr Asp Val Thr Gly Thr Leu Gly Pro 
        195                 200                 205             


Val Pro Val Ser Thr Lys Ile Glu Val Asp Pro Phe Ile Leu Ser Leu 
    210                 215                 220                 


Gly Ala Ser Tyr Val Phe 
225                 230 


