                               SEQUENCE LISTING

<110> UNIVERSITY OF SOUTHERN CALIFORNIA
 
<120> SECRETORY TNT CAR CELL IMMUNOTHERAPY

<130> 064189-7181

<140>
<141>

<150> 62/155,296
<151> 2015-04-30

<160> 68    

<170> PatentIn version 3.5

<210> 1
<211> 8
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 1
Gly Phe Ser Leu Thr Asp Tyr Gly 
1               5               


<210> 2
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 2
Ile Trp Gly Gly Gly Ser Thr 
1               5           


<210> 3
<211> 14
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 3
Ala Lys Glu Lys Arg Arg Gly Tyr Tyr Tyr Ala Met Asp Tyr 
1               5                   10                  


<210> 4
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 4
Ser Ser Val Ser Ser Ser Tyr 
1               5           


<210> 5
<211> 3
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 5
Ser Thr Ser 
1           


<210> 6
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 6
Gln Gln Tyr Ser Gly Tyr Pro Leu Thr 
1               5                   


<210> 7
<211> 360
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 7
caggtgcagc tgaaggagtc aggacctggc ctggtggcgc cctcacagag cctgtccatc       60

acatgcactg tctcagggtt ctcattaacc gactatggtg taaggtggat tcgccagcct      120

ccaggaaagg gtctggagtg gctgggagta atatggggtg gtggaagcac atactataat      180

tcagctctca aatccagact gagcatcagc aaggacaact ccaagagcca agttttctta      240

aaaatgaaca gtctgcaaac tgatgacaca gccatgtact actgtgccaa agagaaacgg      300

agggggtatt actatgctat ggactactgg ggtcaaggaa cctcagtcac cgtctcctca      360


<210> 8
<211> 327
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 8
ggagaaaatg tgctcaccca gtctccagca atcatgtctg catctccagg ggaaaaggtc       60

accatgacct gcagggccag ctcaagtgta agttccagtt acttgcactg gtaccagcag      120

aagtcaggtg cctcccccaa actctggatt tatagcacat ccaacttggc ttctggagtc      180

cctgctcgct tcagtggcag tgggtctggg acctcttact ctctcacaat cagcagtgtg      240

gaggctgaag atgctgccac ttattactgc cagcagtaca gtggttaccc actcacgttc      300

ggagggggga ccaagctgga aataaaa                                          327


<210> 9
<211> 8
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 9
Gly Tyr Ser Phe Thr Gly Tyr Tyr 
1               5               


<210> 10
<211> 8
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 10
Ile Asn Pro Tyr Asn Gly Ala Thr 
1               5               


<210> 11
<211> 8
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 11
Ala Arg Leu Asp Arg Gly Asp Tyr 
1               5               


<210> 12
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 12
Glu Asn Val Val Thr Tyr 
1               5       


<210> 13
<211> 3
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 13
Gly Ala Ser 
1           


<210> 14
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 14
Gly Gln Gly Tyr Ser Tyr Pro Tyr Thr 
1               5                   


<210> 15
<211> 345
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 15
gaggtacagc tgcagcagtc tggacctgag ctggtgaagc ctggggcttc agtgaagata       60

tcctgcaagg cttctggtta ctcattcact ggctactaca tgcactgggt gaagcaaagc      120

catgtaaaga gccttgagtg gattggacgt attaatcctt acaatggtgc tactagctac      180

aaccagaatt tcaaggacaa ggccagcttg actgtagata agtcctccag cacagcctac      240

atggagctcc acagcctgac atctgaggac tctgcagtct attactgtgc aagactagac      300

cggggggact actggggtca aggaacctca gtcaccgtct cctca                      345


<210> 16
<211> 327
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 16
aacattgtaa tgacccaatc tcccaaatcc atgtccatgt cagtaggaga gagggtcacc       60

ttgacctgca aggccagtga gaatgtggtt acttatgttt cctggtatca acagaaacca      120

gagcagtctc ctaaactgct gatatacggg gcatccaacc ggtacactgg ggtccccgat      180

cgcttcacag gcagtggatc tgcaacagat ttcactctga ccatcagcag tgtgcaggct      240

gaagaccttg cagattatca ctgtggacag ggttacagct atccgtacac gttcggaggg      300

gggacaaagt tggaaataaa acgtacg                                          327


<210> 17
<211> 8
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 17
Gly Tyr Thr Phe Thr Arg Tyr Trp 
1               5               


<210> 18
<211> 8
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 18
Ile Tyr Pro Gly Asn Ser Asp Thr 
1               5               


<210> 19
<211> 14
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 19
Ala Arg Gly Glu Glu Ile Gly Val Arg Arg Trp Phe Ala Tyr 
1               5                   10                  


<210> 20
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 20
Gln Ser Ile Ser Asn Tyr 
1               5       


<210> 21
<211> 3
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 21
Tyr Ala Ser 
1           


<210> 22
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 22
Gln Gln Ser Asn Ser Trp Pro Leu Thr 
1               5                   


<210> 23
<211> 363
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 23
caggtccaac tgcagcagtc aggagctgaa ctggtcaaga ctggggcctc agtgaagatg       60

tcctgcaagg cttctggcta cacctttacc agatactgga tgcactgggt aaaacagagg      120

cctggacagg ctctggaatg gattggcgct atttatcctg gaaatagtga tactagctac      180

taccagaagt tcaagggcaa ggccaaactg actgcagtca catctgccag cactgcctac      240

atggagctca gcagcctgac atctgaggac tctgccgtct attactgtgc aagaggggag      300

gaaatagggg tacgacgctg gtttgcttac tggggccaag ggactctggt cactgtctct      360

gca                                                                    363


<210> 24
<211> 321
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 24
gatattgtgc taactcagtc tccagccacc ctgtctgtga ctccaggaga tagagtcagt       60

ctttcctgca gggccaggca aagtattagc aactacctac actggtatca acaaaaatca      120

catgagtctc caaggcttct catcaagtat gcttcccagt ccatctctgg catcccctcc      180

aggttcagtg gcagtggatc agggacagat ttcactctca gtatcaacag tgtggagact      240

gaagattttg gaatgtattt ctgtcaacag agtaacagct ggccgctcac gttcggtgct      300

gggaccaagc tggaaataaa a                                                321


<210> 25
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 25
Ser Gly Tyr Tyr Trp Gly 
1               5       


<210> 26
<211> 16
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 26
Ser Ile Tyr His Ser Gly Ser Thr Tyr Tyr Asn Pro Ser Leu Lys Ser 
1               5                   10                  15      


<210> 27
<211> 8
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 27
Gly Lys Trp Ser Lys Phe Asp Tyr 
1               5               


<210> 28
<211> 11
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 28
Gln Gly Asp Ser Leu Arg Ser Tyr Tyr Ala Ser 
1               5                   10      


<210> 29
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 29
Gly Lys Asn Asn Arg Pro Ser 
1               5           


<210> 30
<211> 11
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 30
Asn Ser Arg Asp Ser Ser Gly Asn His Val Val 
1               5                   10      


<210> 31
<211> 351
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 31
caggtgcagc tgcaggagtc cggcccagga ctggtgaagc cttcggagac cctgtccctc       60

acctgcgctg tctctggtta ctccatcagc agtggttact actggggctg gattcggcag      120

cccccaggga aggggctgga gtggattggg agtatctatc atagtgggag cacctactac      180

aacccgtccc tcaagagtcg agtcaccata tcagtagaca cgtccaagaa ccagttctcc      240

ctgaagctga gctctgtgac cgccgcagac acggccgtgt attactgtgc aagagggaag      300

tggtcgaagt ttgactattg gggccaaggc accctggtca ccgtctcttc a               351


<210> 32
<211> 324
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 32
tcctctgagc tgactcagga ccctgctgtg tctgtggcct tgggacagac agtcaggatc       60

acatgccaag gagacagcct cagaagctat tatgcaagct ggtaccagca gaagccagga      120

caggcccctg tacttgtcat ctatggtaaa aacaaccggc cctcagggat tccagaccga      180

ttctctggct ccagctcagg aaacacagct tccttgacca tcactggggc tcaggcggaa      240

gatgaggctg actattactg taactcccgg gacagcagtg gtaaccatgt ggtattcggc      300

ggagggacca agctgaccgt ccta                                             324


<210> 33
<211> 51
<212> PRT
<213> Homo sapiens

<400> 33
Pro Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala 
1               5                   10                  15      


Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg 
            20                  25                  30          


Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys 
        35                  40                  45              


Asp Ile Tyr 
    50      


<210> 34
<211> 49
<212> PRT
<213> Mus musculus

<400> 34
Lys Val Asn Ser Thr Thr Thr Lys Pro Val Leu Arg Thr Pro Ser Pro 
1               5                   10                  15      


Val His Pro Thr Gly Thr Ser Gln Pro Gln Arg Pro Glu Asp Cys Arg 
            20                  25                  30          


Pro Arg Gly Ser Val Lys Gly Thr Gly Leu Asp Phe Ala Cys Asp Ile 
        35                  40                  45              


Tyr 
    


<210> 35
<211> 51
<212> PRT
<213> Felis catus

<400> 35
Pro Val Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Gln Ala 
1               5                   10                  15      


Pro Ile Thr Thr Ser Gln Arg Val Ser Leu Arg Pro Gly Thr Cys Gln 
            20                  25                  30          


Pro Ser Ala Gly Ser Thr Val Glu Ala Ser Gly Leu Asp Leu Ser Cys 
        35                  40                  45              


Asp Ile Tyr 
    50      


<210> 36
<211> 21
<212> PRT
<213> Homo sapiens

<400> 36
Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr 
            20      


<210> 37
<211> 21
<212> PRT
<213> Mus musculus

<400> 37
Ile Trp Ala Pro Leu Ala Gly Ile Cys Val Ala Leu Leu Leu Ser Leu 
1               5                   10                  15      


Ile Ile Thr Leu Ile 
            20      


<210> 38
<211> 21
<212> PRT
<213> Rattus norvegicus

<400> 38
Ile Trp Ala Pro Leu Ala Gly Ile Cys Ala Val Leu Leu Leu Ser Leu 
1               5                   10                  15      


Val Ile Thr Leu Ile 
            20      


<210> 39
<211> 42
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      4-1BB costimulatory signaling region

<400> 39
Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210> 40
<211> 220
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      CD28 polypeptide

<400> 40
Met Leu Arg Leu Leu Leu Ala Leu Asn Leu Phe Pro Ser Ile Gln Val 
1               5                   10                  15      


Thr Gly Asn Lys Ile Leu Val Lys Gln Ser Pro Met Leu Val Ala Tyr 
            20                  25                  30          


Asp Asn Ala Val Asn Leu Ser Cys Lys Tyr Ser Tyr Asn Leu Phe Ser 
        35                  40                  45              


Arg Glu Phe Arg Ala Ser Leu His Lys Gly Leu Asp Ser Ala Val Glu 
    50                  55                  60                  


Val Cys Val Val Tyr Gly Asn Tyr Ser Gln Gln Leu Gln Val Tyr Ser 
65                  70                  75                  80  


Lys Thr Gly Phe Asn Cys Asp Gly Lys Leu Gly Asn Glu Ser Val Thr 
                85                  90                  95      


Phe Tyr Leu Gln Asn Leu Tyr Val Asn Gln Thr Asp Ile Tyr Phe Cys 
            100                 105                 110         


Lys Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser 
        115                 120                 125             


Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro 
    130                 135                 140                 


Leu Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly 
145                 150                 155                 160 


Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile 
                165                 170                 175     


Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met 
            180                 185                 190         


Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro 
        195                 200                 205             


Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
    210                 215                 220 


<210> 41
<211> 112
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      CD3 zeta signaling domain

<400> 41
Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210> 42
<211> 288
<212> PRT
<213> Homo sapiens

<400> 42
Met Gly His Thr Arg Arg Gln Gly Thr Ser Pro Ser Lys Cys Pro Tyr 
1               5                   10                  15      


Leu Asn Phe Phe Gln Leu Leu Val Leu Ala Gly Leu Ser His Phe Cys 
            20                  25                  30          


Ser Gly Val Ile His Val Thr Lys Glu Val Lys Glu Val Ala Thr Leu 
        35                  40                  45              


Ser Cys Gly His Asn Val Ser Val Glu Glu Leu Ala Gln Thr Arg Ile 
    50                  55                  60                  


Tyr Trp Gln Lys Glu Lys Lys Met Val Leu Thr Met Met Ser Gly Asp 
65                  70                  75                  80  


Met Asn Ile Trp Pro Glu Tyr Lys Asn Arg Thr Ile Phe Asp Ile Thr 
                85                  90                  95      


Asn Asn Leu Ser Ile Val Ile Leu Ala Leu Arg Pro Ser Asp Glu Gly 
            100                 105                 110         


Thr Tyr Glu Cys Val Val Leu Lys Tyr Glu Lys Asp Ala Phe Lys Arg 
        115                 120                 125             


Glu His Leu Ala Glu Val Thr Leu Ser Val Lys Ala Asp Phe Pro Thr 
    130                 135                 140                 


Pro Ser Ile Ser Asp Phe Glu Ile Pro Thr Ser Asn Ile Arg Arg Ile 
145                 150                 155                 160 


Ile Cys Ser Thr Ser Gly Gly Phe Pro Glu Pro His Leu Ser Trp Leu 
                165                 170                 175     


Glu Asn Gly Glu Glu Leu Asn Ala Ile Asn Thr Thr Val Ser Gln Asp 
            180                 185                 190         


Pro Glu Thr Glu Leu Tyr Ala Val Ser Ser Lys Leu Asp Phe Asn Met 
        195                 200                 205             


Thr Thr Asn His Ser Phe Met Cys Leu Ile Lys Tyr Gly His Leu Arg 
    210                 215                 220                 


Val Asn Gln Thr Phe Asn Trp Asn Thr Thr Lys Gln Glu His Phe Pro 
225                 230                 235                 240 


Asp Asn Leu Leu Pro Ser Trp Ala Ile Thr Leu Ile Ser Val Asn Gly 
                245                 250                 255     


Ile Phe Val Ile Cys Cys Leu Thr Tyr Cys Phe Ala Pro Arg Cys Arg 
            260                 265                 270         


Glu Arg Arg Arg Asn Glu Arg Leu Arg Arg Glu Ser Val Arg Pro Val 
        275                 280                 285             


<210> 43
<211> 98
<212> PRT
<213> Homo sapiens

<400> 43
Met Ala Leu Leu Leu Ala Leu Ser Leu Leu Val Leu Trp Thr Ser Pro 
1               5                   10                  15      


Ala Pro Thr Leu Ser Gly Thr Asn Asp Ala Glu Asp Cys Cys Leu Ser 
            20                  25                  30          


Val Thr Gln Lys Pro Ile Pro Gly Tyr Ile Val Arg Asn Phe His Tyr 
        35                  40                  45              


Leu Leu Ile Lys Asp Gly Cys Arg Val Pro Ala Val Val Phe Thr Thr 
    50                  55                  60                  


Leu Arg Gly Arg Gln Leu Cys Ala Pro Pro Asp Gln Pro Trp Val Glu 
65                  70                  75                  80  


Arg Ile Ile Gln Arg Leu Gln Arg Thr Ser Ala Lys Met Lys Arg Arg 
                85                  90                  95      


Ser Ser 
        


<210> 44
<211> 96
<212> PRT
<213> Homo sapiens

<400> 44
Met Cys Cys Thr Lys Ser Leu Leu Leu Ala Ala Leu Met Ser Val Leu 
1               5                   10                  15      


Leu Leu His Leu Cys Gly Glu Ser Glu Ala Ala Ser Asn Phe Asp Cys 
            20                  25                  30          


Cys Leu Gly Tyr Thr Asp Arg Ile Leu His Pro Lys Phe Ile Val Gly 
        35                  40                  45              


Phe Thr Arg Gln Leu Ala Asn Glu Gly Cys Asp Ile Asn Ala Ile Ile 
    50                  55                  60                  


Phe His Thr Lys Lys Lys Leu Ser Val Cys Ala Asn Pro Lys Gln Thr 
65                  70                  75                  80  


Trp Val Lys Tyr Ile Val Arg Leu Leu Ser Lys Lys Val Lys Asn Met 
                85                  90                  95      


<210> 45
<211> 95
<212> PRT
<213> Homo sapiens

<400> 45
Met Cys Cys Thr Lys Ser Leu Leu Leu Ala Ala Leu Met Ser Val Leu 
1               5                   10                  15      


Leu Leu His Leu Cys Gly Glu Ser Glu Ala Ser Asn Phe Asp Cys Cys 
            20                  25                  30          


Leu Gly Tyr Thr Asp Arg Ile Leu His Pro Lys Phe Ile Val Gly Phe 
        35                  40                  45              


Thr Arg Gln Leu Ala Asn Glu Gly Cys Asp Ile Asn Ala Ile Ile Phe 
    50                  55                  60                  


His Thr Lys Lys Lys Leu Ser Val Cys Ala Asn Pro Lys Gln Thr Trp 
65                  70                  75                  80  


Val Lys Tyr Ile Val Arg Leu Leu Ser Lys Lys Val Lys Asn Met 
                85                  90                  95  


<210> 46
<211> 261
<212> PRT
<213> Homo sapiens

<400> 46
Met Ile Glu Thr Tyr Asn Gln Thr Ser Pro Arg Ser Ala Ala Thr Gly 
1               5                   10                  15      


Leu Pro Ile Ser Met Lys Ile Phe Met Tyr Leu Leu Thr Val Phe Leu 
            20                  25                  30          


Ile Thr Gln Met Ile Gly Ser Ala Leu Phe Ala Val Tyr Leu His Arg 
        35                  40                  45              


Arg Leu Asp Lys Ile Glu Asp Glu Arg Asn Leu His Glu Asp Phe Val 
    50                  55                  60                  


Phe Met Lys Thr Ile Gln Arg Cys Asn Thr Gly Glu Arg Ser Leu Ser 
65                  70                  75                  80  


Leu Leu Asn Cys Glu Glu Ile Lys Ser Gln Phe Glu Gly Phe Val Lys 
                85                  90                  95      


Asp Ile Met Leu Asn Lys Glu Glu Thr Lys Lys Glu Asn Ser Phe Glu 
            100                 105                 110         


Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser 
        115                 120                 125             


Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly 
    130                 135                 140                 


Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln 
145                 150                 155                 160 


Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr 
                165                 170                 175     


Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser 
            180                 185                 190         


Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala 
        195                 200                 205             


Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His 
    210                 215                 220                 


Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn 
225                 230                 235                 240 


Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe 
                245                 250                 255     


Gly Leu Leu Lys Leu 
            260     


<210> 47
<211> 254
<212> PRT
<213> Homo sapiens

<400> 47
Met Glu Tyr Ala Ser Asp Ala Ser Leu Asp Pro Glu Ala Pro Trp Pro 
1               5                   10                  15      


Pro Ala Pro Arg Ala Arg Ala Cys Arg Val Leu Pro Trp Ala Leu Val 
            20                  25                  30          


Ala Gly Leu Leu Leu Leu Leu Leu Leu Ala Ala Ala Cys Ala Val Phe 
        35                  40                  45              


Leu Ala Cys Pro Trp Ala Val Ser Gly Ala Arg Ala Ser Pro Gly Ser 
    50                  55                  60                  


Ala Ala Ser Pro Arg Leu Arg Glu Gly Pro Glu Leu Ser Pro Asp Asp 
65                  70                  75                  80  


Pro Ala Gly Leu Leu Asp Leu Arg Gln Gly Met Phe Ala Gln Leu Val 
                85                  90                  95      


Ala Gln Asn Val Leu Leu Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp 
            100                 105                 110         


Pro Gly Leu Ala Gly Val Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu 
        115                 120                 125             


Asp Thr Lys Glu Leu Val Val Ala Lys Ala Gly Val Tyr Tyr Val Phe 
    130                 135                 140                 


Phe Gln Leu Glu Leu Arg Arg Val Val Ala Gly Glu Gly Ser Gly Ser 
145                 150                 155                 160 


Val Ser Leu Ala Leu His Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala 
                165                 170                 175     


Ala Ala Leu Ala Leu Thr Val Asp Leu Pro Pro Ala Ser Ser Glu Ala 
            180                 185                 190         


Arg Asn Ser Ala Phe Gly Phe Gln Gly Arg Leu Leu His Leu Ser Ala 
        195                 200                 205             


Gly Gln Arg Leu Gly Val His Leu His Thr Glu Ala Arg Ala Arg His 
    210                 215                 220                 


Ala Trp Gln Leu Thr Gln Gly Ala Thr Val Leu Gly Leu Phe Arg Val 
225                 230                 235                 240 


Thr Pro Glu Ile Pro Ala Gly Leu Pro Ser Pro Arg Ser Glu 
                245                 250                 


<210> 48
<211> 199
<212> PRT
<213> Homo sapiens

<400> 48
Met Thr Leu His Pro Ser Pro Ile Thr Cys Glu Phe Leu Phe Ser Thr 
1               5                   10                  15      


Ala Leu Ile Ser Pro Lys Met Cys Leu Ser His Leu Glu Asn Met Pro 
            20                  25                  30          


Leu Ser His Ser Arg Thr Gln Gly Ala Gln Arg Ser Ser Trp Lys Leu 
        35                  40                  45              


Trp Leu Phe Cys Ser Ile Val Met Leu Leu Phe Leu Cys Ser Phe Ser 
    50                  55                  60                  


Trp Leu Ile Phe Ile Phe Leu Gln Leu Glu Thr Ala Lys Glu Pro Cys 
65                  70                  75                  80  


Met Ala Lys Phe Gly Pro Leu Pro Ser Lys Trp Gln Met Ala Ser Ser 
                85                  90                  95      


Glu Pro Pro Cys Val Asn Lys Val Ser Asp Trp Lys Leu Glu Ile Leu 
            100                 105                 110         


Gln Asn Gly Leu Tyr Leu Ile Tyr Gly Gln Val Ala Pro Asn Ala Asn 
        115                 120                 125             


Tyr Asn Asp Val Ala Pro Phe Glu Val Arg Leu Tyr Lys Asn Lys Asp 
    130                 135                 140                 


Met Ile Gln Thr Leu Thr Asn Lys Ser Lys Ile Gln Asn Val Gly Gly 
145                 150                 155                 160 


Thr Tyr Glu Leu His Val Gly Asp Thr Ile Asp Leu Ile Phe Asn Ser 
                165                 170                 175     


Glu His Gln Val Leu Lys Asn Asn Thr Tyr Trp Gly Ile Ile Leu Leu 
            180                 185                 190         


Ala Asn Pro Gln Phe Ile Ser 
        195                 


<210> 49
<211> 144
<212> PRT
<213> Homo sapiens

<400> 49
Met Trp Leu Gln Ser Leu Leu Leu Leu Gly Thr Val Ala Cys Ser Ile 
1               5                   10                  15      


Ser Ala Pro Ala Arg Ser Pro Ser Pro Ser Thr Gln Pro Trp Glu His 
            20                  25                  30          


Val Asn Ala Ile Gln Glu Ala Arg Arg Leu Leu Asn Leu Ser Arg Asp 
        35                  40                  45              


Thr Ala Ala Glu Met Asn Glu Thr Val Glu Val Ile Ser Glu Met Phe 
    50                  55                  60                  


Asp Leu Gln Glu Pro Thr Cys Leu Gln Thr Arg Leu Glu Leu Tyr Lys 
65                  70                  75                  80  


Gln Gly Leu Arg Gly Ser Leu Thr Lys Leu Lys Gly Pro Leu Thr Met 
                85                  90                  95      


Met Ala Ser His Tyr Lys Gln His Cys Pro Pro Thr Pro Glu Thr Ser 
            100                 105                 110         


Cys Ala Thr Gln Ile Ile Thr Phe Glu Ser Phe Lys Glu Asn Leu Lys 
        115                 120                 125             


Asp Phe Leu Leu Val Ile Pro Phe Asp Cys Trp Glu Pro Val Gln Glu 
    130                 135                 140                 


<210> 50
<211> 1611
<212> DNA
<213> Homo sapiens

<400> 50
catggccatg tgtcatcagc agctggtcat cagctggttc agcctggtgt tcctggccag       60

ccccctggtg gccatctggg agctgaagaa agacgtgtac gtggtggagc tggactggta      120

tcccgacgcc cctggcgaga tggtggtgct gacctgcgac acccccgaag aggacggcat      180

cacctggacc ctggaccaga gcagcgaggt gctgggcagc ggcaagaccc tgaccatcca      240

ggtcaaagag ttcggcgacg ccggccagta cacctgccac aagggcggcg aagtgctgtc      300

ccacagcctg ctgctgctgc acaagaaaga ggatggcatc tggtccaccg acatcctgaa      360

ggaccagaaa gagcccaaga acaagacctt cctgcggtgc gaggccaaga actacagcgg      420

ccggttcacc tgttggtggc tgaccaccat cagcaccgac ctgaccttca gcgtgaagag      480

cagccggggc agcagcgacc ctcagggcgt gacctgcgga gccgccaccc tgagcgccga      540

gagagtgcgg ggcgacaaca aagagtacga gtacagcgtc gagtgccagg aagatagcgc      600

ctgccctgcc gccgaggaaa gcctgcccat cgaggtgatg gtggacgccg tgcacaagct      660

gaagtacgag aactacacct ccagcttttt catccgggac atcatcaagc ccgacccccc      720

caagaacctg cagctgaagc ccctgaagaa cagccggcag gtggaggtgt cctgggagta      780

ccctgacacc tggtccaccc cccacagcta cttcagcctg accttctgtg tgcaggtgca      840

gggcaagagc aagcgggaga agaaagaccg ggtgttcacc gacaagacca gcgccaccgt      900

gatctgccgg aagaacgcca gcatcagcgt gcgggcccag gaccggtact acagcagctc      960

ctggtccgag tgggccagcg tgccctgcag cggcggaggg ggcggaggaa gccggaacct     1020

gcccgtggct acccccgacc ccggcatgtt cccctgcctg caccacagcc agaacctgct     1080

gcgggccgtg agcaacatgc tgcagaaggc ccggcagacc ctggaattct acccctgcac     1140

cagcgaggaa atcgaccacg aggacatcac caaggataag accagcaccg tggaggcctg     1200

cctgcccctg gaactgacca agaacgagag ctgtctgaac tctcgggaga caagcttcat     1260

caccaacggc tcttgcctgg ccagcagaaa gaccagcttc atgatggccc tgtgcctgag     1320

cagcatctac gaggacctga agatgtacca ggtggagttc aagaccatga acgccaagct     1380

gctgatggac cccaagcggc agatcttcct ggatcagaac atgctggccg tgatcgacga     1440

gctgatgcag gccctgaact tcaacagcga gacagtgccc cagaagtcca gcctggaaga     1500

gcccgacttc tacaagacca agatcaagct gtgcatcctc ctgcatgcct tccggatccg     1560

ggccgtgacc atcgaccggg tgatgagcta cctgaacgcc agctgatgag c              1611


<210> 51
<211> 133
<212> PRT
<213> Homo sapiens

<400> 51
Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210> 52
<211> 113
<212> PRT
<213> Homo sapiens

<400> 52
Trp Val Asn Val Ile Ser Asp Leu Lys Lys Ile Glu Asp Leu Ile Gln 
1               5                   10                  15      


Ser Met His Ile Asp Ala Thr Leu Tyr Thr Glu Ser Asp Val His Pro 
            20                  25                  30          


Ser Cys Lys Val Thr Ala Met Lys Cys Phe Leu Leu Glu Leu Gln Val 
        35                  40                  45              


Ile Ser Leu Glu Ser Gly Asp Ala Ser Ile His Asp Thr Val Glu Asn 
    50                  55                  60                  


Leu Ile Ile Leu Ala Asn Asn Ser Leu Ser Ser Asn Gly Asn Val Thr 
65                  70                  75                  80  


Glu Ser Gly Cys Lys Glu Cys Glu Glu Leu Glu Lys Lys Asn Ile Lys 
                85                  90                  95      


Glu Phe Leu Gln Ser Phe Val His Ile Val Gln Met Phe Ile Asn Thr 
            100                 105                 110         


Ser 
    


<210> 53
<211> 193
<212> PRT
<213> Homo sapiens

<400> 53
Met Ala Ala Glu Pro Val Glu Asp Asn Cys Ile Asn Phe Val Ala Met 
1               5                   10                  15      


Lys Phe Ile Asp Asn Thr Leu Tyr Phe Ile Ala Glu Asp Asp Glu Asn 
            20                  25                  30          


Leu Glu Ser Asp Tyr Phe Gly Lys Leu Glu Ser Lys Leu Ser Val Ile 
        35                  40                  45              


Arg Asn Leu Asn Asp Gln Val Leu Phe Ile Asp Gln Gly Asn Arg Pro 
    50                  55                  60                  


Leu Phe Glu Asp Met Thr Asp Ser Asp Cys Arg Asp Asn Ala Pro Arg 
65                  70                  75                  80  


Thr Ile Phe Ile Ile Ser Met Tyr Lys Asp Ser Gln Pro Arg Gly Met 
                85                  90                  95      


Ala Val Thr Ile Ser Val Lys Cys Glu Lys Ile Ser Thr Leu Ser Cys 
            100                 105                 110         


Glu Asn Lys Ile Ile Ser Phe Lys Glu Met Asn Pro Pro Asp Asn Ile 
        115                 120                 125             


Lys Asp Thr Lys Ser Asp Ile Ile Phe Phe Gln Arg Ser Val Pro Gly 
    130                 135                 140                 


His Asp Asn Lys Met Gln Phe Glu Ser Ser Ser Tyr Glu Gly Tyr Phe 
145                 150                 155                 160 


Leu Ala Cys Glu Lys Glu Arg Asp Leu Phe Lys Leu Ile Leu Lys Lys 
                165                 170                 175     


Glu Asp Glu Leu Gly Asp Arg Ser Ile Met Phe Thr Val Gln Asn Glu 
            180                 185                 190         


Asp 
    


<210> 54
<211> 133
<212> PRT
<213> Homo sapiens

<400> 54
Gln Gly Gln Asp Arg His Met Ile Arg Met Arg Gln Leu Ile Asp Ile 
1               5                   10                  15      


Val Asp Gln Leu Lys Asn Tyr Val Asn Asp Leu Val Pro Glu Phe Leu 
            20                  25                  30          


Pro Ala Pro Glu Asp Val Glu Thr Asn Cys Glu Trp Ser Ala Phe Ser 
        35                  40                  45              


Cys Phe Gln Lys Ala Gln Leu Lys Ser Ala Asn Thr Gly Asn Asn Glu 
    50                  55                  60                  


Arg Ile Ile Asn Val Ser Ile Lys Lys Leu Lys Arg Lys Pro Pro Ser 
65                  70                  75                  80  


Thr Asn Ala Gly Arg Arg Gln Lys His Arg Leu Thr Cys Pro Ser Cys 
                85                  90                  95      


Asp Ser Tyr Glu Lys Lys Pro Pro Lys Glu Phe Leu Glu Arg Phe Lys 
            100                 105                 110         


Ser Leu Leu Gln Lys Met Ile His Gln His Leu Ser Ser Arg Thr His 
        115                 120                 125             


Gly Ser Glu Asp Ser 
    130             


<210> 55
<211> 120
<212> PRT
<213> Homo sapiens

<400> 55
Met Lys Val Ser Glu Ala Ala Leu Ser Leu Leu Val Leu Ile Leu Ile 
1               5                   10                  15      


Ile Thr Ser Ala Ser Arg Ser Gln Pro Lys Val Pro Glu Trp Val Asn 
            20                  25                  30          


Thr Pro Ser Thr Cys Cys Leu Lys Tyr Tyr Glu Lys Val Leu Pro Arg 
        35                  40                  45              


Arg Leu Val Val Gly Tyr Arg Lys Ala Leu Asn Cys His Leu Pro Ala 
    50                  55                  60                  


Ile Ile Phe Val Thr Lys Arg Asn Arg Glu Val Cys Thr Asn Pro Asn 
65                  70                  75                  80  


Asp Asp Trp Val Gln Glu Tyr Ile Lys Asp Pro Asn Leu Pro Leu Leu 
                85                  90                  95      


Pro Thr Arg Asn Leu Ser Thr Val Lys Ile Ile Thr Ala Lys Asn Gly 
            100                 105                 110         


Gln Pro Gln Leu Leu Asn Ser Gln 
        115                 120 


<210> 56
<211> 183
<212> PRT
<213> Homo sapiens

<400> 56
Met Glu Arg Val Gln Pro Leu Glu Glu Asn Val Gly Asn Ala Ala Arg 
1               5                   10                  15      


Pro Arg Phe Glu Arg Asn Lys Leu Leu Leu Val Ala Ser Val Ile Gln 
            20                  25                  30          


Gly Leu Gly Leu Leu Leu Cys Phe Thr Tyr Ile Cys Leu His Phe Ser 
        35                  40                  45              


Ala Leu Gln Val Ser His Arg Tyr Pro Arg Ile Gln Ser Ile Lys Val 
    50                  55                  60                  


Gln Phe Thr Glu Tyr Lys Lys Glu Lys Gly Phe Ile Leu Thr Ser Gln 
65                  70                  75                  80  


Lys Glu Asp Glu Ile Met Lys Val Gln Asn Asn Ser Val Ile Ile Asn 
                85                  90                  95      


Cys Asp Gly Phe Tyr Leu Ile Ser Leu Lys Gly Tyr Phe Ser Gln Glu 
            100                 105                 110         


Val Asn Ile Ser Leu His Tyr Gln Lys Asp Glu Glu Pro Leu Phe Gln 
        115                 120                 125             


Leu Lys Lys Val Arg Ser Val Asn Ser Leu Met Val Ala Ser Leu Thr 
    130                 135                 140                 


Tyr Lys Asp Lys Val Tyr Leu Asn Val Thr Thr Asp Asn Thr Ser Leu 
145                 150                 155                 160 


Asp Asp Phe His Val Asn Gly Gly Glu Leu Ile Leu Ile His Gln Asn 
                165                 170                 175     


Pro Gly Glu Phe Cys Val Leu 
            180             


<210> 57
<211> 29
<212> DNA
<213> Homo sapiens

<400> 57
ggaggaaaaa ctgtttcata cagaaggcg                                         29


<210> 58
<211> 9
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 58
aggaaaaac                                                                9


<210> 59
<211> 48
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      IgG1 heavy chain hinge sequence

<400> 59
ctcgagccca aatcttgtga caaaactcac acatgcccac cgtgcccg                    48


<210> 60
<211> 81
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      CD28 transmembrane region

<400> 60
ttttgggtgc tggtggtggt tggtggagtc ctggcttgct atagcttgct agtaacagtg       60

gcctttatta ttttctgggt g                                                 81


<210> 61
<211> 126
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      4-1BB co-stimulatory signaling region

<400> 61
aaacggggca gaaagaaact cctgtatata ttcaaacaac catttatgag accagtacaa       60

actactcaag aggaagatgg ctgtagctgc cgatttccag aagaagaaga aggaggatgt      120

gaactg                                                                 126


<210> 62
<211> 123
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      CD28 co-stimulatory signaling region

<400> 62
aggagtaaga ggagcaggct cctgcacagt gactacatga acatgactcc ccgccgcccc       60

gggcccaccc gcaagcatta ccagccctat gccccaccac gcgacttcgc agcctatcgc      120

tcc                                                                    123


<210> 63
<211> 339
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      CD3 zeta signaling region

<400> 63
agagtgaagt tcagcaggag cgcagacgcc cccgcgtacc agcagggcca gaaccagctc       60

tataacgagc tcaatctagg acgaagagag gagtacgatg ttttggacaa gagacgtggc      120

cgggaccctg agatgggggg aaagccgaga aggaagaacc ctcaggaagg cctgtacaat      180

gaactgcaga aagataagat ggcggaggcc tacagtgaga ttgggatgaa aggcgagcgc      240

cggaggggca aggggcacga tggcctttac cagggtctca gtacagccac caaggacacc      300

tacgacgccc ttcacatgca ggccctgccc cctcgctaa                             339


<210> 64
<211> 105
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      ICOS costimulatory signaling region

<400> 64
acaaaaaaga agtattcatc cagtgtgcac gaccctaacg gtgaatacat gttcatgaga       60

gcagtgaaca cagccaaaaa atccagactc acagatgtga cccta                      105


<210> 65
<211> 108
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      OX40 costimulatory signaling region

<400> 65
agggaccaga ggctgccccc cgatgcccac aagccccctg ggggaggcag tttccggacc       60

cccatccaag aggagcaggc cgacgcccac tccaccctgg ccaagatc                   108


<210> 66
<211> 12
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220> 
<221> MISC_FEATURE
<222> (1)..(12)
<223> This sequence may encompass 1-6 repeating "Gly Ser" units

<400> 66
Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser 
1               5                   10          


<210> 67
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 67
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210> 68
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 68
Gly Gly Gly Gly Ser 
1               5   


