                         SEQUENCE LISTING

<110>  The Trustees of the University of Pennsylvania
 
<120>  DUAL AAV VECTOR SYSTEM FOR CRISPR/Cas9 MEDIATED CORRECTION OF 
       HUMAN DISEASE

<130>  UPN-15-7506PCT

<150>  US 62/153,470
<151>  2015-04-27

<150>  US 62/183,825
<151>  2015-06-24

<150>  US 62/254,225
<151>  2015-11-12

<150>  US 62/287,511
<151>  2016-01-27

<160>  198   

<170>  PatentIn version 3.5

<210>  1
<211>  120
<212>  DNA
<213>  Mus musculus


<220>
<221>  misc_feature
<222>  (4)..(23)
<223>  sgRNA2

<220>
<221>  misc_feature
<222>  (24)..(29)
<223>  PAM

<220>
<221>  misc_feature
<222>  (52)..(52)
<223>  spfash mutation site G->A

<220>
<221>  misc_feature
<222>  (77)..(96)
<223>  sgRNA3

<220>
<221>  misc_feature
<222>  (91)..(96)
<223>  PAM (reverse complement sequence)

<220>
<221>  misc_feature
<222>  (97)..(102)
<223>  PAM

<220>
<221>  misc_feature
<222>  (97)..(116)
<223>  sgRNA1 (reverse complement sequence)

<400>  1
ccacacaaga cattcacttg ggtgtgaatg aaagtctcac agacaccgct cggtttgtaa       60

aacttttctt ccttccaaag tttatttcaa actctgatgg gttagtttaa aagagaagat      120


<210>  2
<211>  157
<212>  DNA
<213>  Mus musculus


<220>
<221>  misc_feature
<222>  (3)..(21)
<223>  sgRNA1

<220>
<221>  misc_feature
<222>  (22)..(27)
<223>  PAM

<220>
<221>  misc_feature
<222>  (98)..(103)
<223>  PAM (reverse complement sequence)

<220>
<221>  misc_feature
<222>  (102)..(104)
<223>  W392X mutation:TGG->TGA

<220>
<221>  misc_feature
<222>  (104)..(123)
<223>  sgRNA3 (reverse complement sequence)

<220>
<221>  misc_feature
<222>  (130)..(149)
<223>  sgRNA2

<220>
<221>  misc_feature
<222>  (150)..(155)
<223>  PAM

<400>  2
caaatggtgg gagctagata ttagggtagg aagccagatg ctaggtatga gagagccaac       60

agcctcagcc ctctgcttgg cttatagatg gagaacaact ctgggcagag gtctcaaagg      120

ctggggctgt gttggacagc aatcatacag tgggtgt                               157


<210>  3
<211>  176
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TBG variant TBG-S1

<400>  3
actcaaagtt caaaccttat cattttttgc tttgttcctc ttggccttgg ttttgtacat       60

cagctttgaa aataccatcc cagggttaat gctggggtta atttataact aagagtgctc      120

tagttttgca atacaggaca tgctataaaa atggaaagat gttgctttct gagaga          176


<210>  4
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  novel enhancer ABPS2

<400>  4
gttaattttt aaactgtttg ctctggttaa taatctcagg aggttaattt ttaaactgtt       60

tgctctggtt aataatctca                                                   80


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  target sequence

<400>  5
agtttgaaat aaactttgga                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  target sequence

<400>  6
gaaaagtttt acaaactgag                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  target sequence

<400>  7
tcagagtttg aaataaactt                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  target sequence

<400>  8
tctcttttaa actaacccat                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  target sequence

<400>  9
cacaagacat tcacttgggt                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  target sequence

<400>  10
aaagtttatt tcaaactctg                                                   20


<210>  11
<211>  92
<212>  DNA
<213>  Mus musculus

<400>  11
gaaagtctca cagacaccgc tcggtttgta aaacttttct tccttccaaa gtttatttca       60

aactctgatg ggttagttta aaagagaaga tg                                     92


<210>  12
<211>  92
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  spfash G->A mutation sequence

<400>  12
gaaagtctca cagacaccgc tcagtttgta aaacttttct tccttccaaa gtttatttca       60

aactctgatg ggttagttta aaagagaaga tg                                     92


<210>  13
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC donor sequence (SpCas9, 892-983 bp)

<400>  13
aaagtctcac agacaccgct cggtttgtaa aacttttctt ccttgcaaag tttatttcaa       60

actctgatgg gttagtttaa aagagaagat g                                      91


<210>  14
<211>  98
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC donor sequence (SaCas9, 892-989 bp)

<400>  14
gaaagtctca cagacaccgc tcggtttgta aaacttttct tccttccaaa gtttaccggt       60

atttcaattg gtgatgggtt agtttaaaag agaagatg                               98


<210>  15
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC sgRNA1_Fwd

<400>  15
caccgtctct tttaaactaa cccat                                             25


<210>  16
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC sgRNA1_Rev

<400>  16
aaacatgggt tagtttaaaa gagac                                             25


<210>  17
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC sgRNA2_Fwd

<400>  17
caccgcacaa gacattcact tgggt                                             25


<210>  18
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC sgRNA2_Rev

<400>  18
aaacacccaa gtgaatgtct tgtgc                                             25


<210>  19
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC sgRNA3_Fwd

<400>  19
caccgaaagt ttatttcaaa ctctg                                             25


<210>  20
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC sgRNA3_Rev

<400>  20
aaaccagagt ttgaaataaa ctttc                                             25


<210>  21
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HDR-Fwd

<400>  21
tggagcaatt ctgcacatgg a                                                 21


<210>  22
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HDR-Rev

<400>  22
cttactgaac atggcagttt ccc                                               23


<210>  23
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_PointM_F

<400>  23
ggctatgctt gggaatgtcc t                                                 21


<210>  24
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_PointM_R

<400>  24
gctacagaat gaaagagagg cg                                                22


<210>  25
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mOTC hSpCas9 sgRNA1_Fwd

<400>  25
caccgagttt gaaataaact ttgga                                             25


<210>  26
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mOTC hSpCas9 sgRNA1_Rev

<400>  26
aaactccaaa gtttatttca aactc                                             25


<210>  27
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mOTC hSpCas9 sgRNA2_Fwd

<400>  27
caccgaaaag ttttacaaac tgag                                              24


<210>  28
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mOTC hSpCas9 sgRNA2_Rev

<400>  28
aaacctcagt ttgtaaaact tttc                                              24


<210>  29
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mOTC hSpCas9 sgRNA3_Fwd

<400>  29
caccgtcaga gtttgaaata aactt                                             25


<210>  30
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mOTC hSpCas9 sgRNA3_Rev

<400>  30
aaacaagttt atttcaaact ctgac                                             25


<210>  31
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mOTC hSaCas9 sgRNA1_Fwd

<400>  31
caccgtctct tttaaactaa cccat                                             25


<210>  32
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mOTC hSaCas9 sgRNA1_Rev

<400>  32
aaacatgggt tagtttaaaa gagac                                             25


<210>  33
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mOTC hSaCas9 sgRNA2_Fwd

<400>  33
caccgcacaa gacattcact tgggt                                             25


<210>  34
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mOTC hSaCas9 sgRNA2_Rev

<400>  34
aaacacccaa gtgaatgtct tgtgc                                             25


<210>  35
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mOTC hSaCas9 sgRNA3_Fwd

<400>  35
caccgaaagt ttatttcaaa ctctg                                             25


<210>  36
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mOTC hSaCas9 sgRNA3_Rev

<400>  36
aaaccagagt ttgaaataaa ctttc                                             25


<210>  37
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT1F1

<400>  37
ctggtgcctt tttctatcgc c                                                 21


<210>  38
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT1R1

<400>  38
ccaagagcaa ctacaatggc tt                                                22


<210>  39
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT1F2

<400>  39
gcattttcat gagcattcca                                                   20


<210>  40
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT1R2

<400>  40
catgttgtgc ctgcatctct                                                   20


<210>  41
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT2F1

<400>  41
gcagactcca agatgcaaga c                                                 21


<210>  42
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT2R1

<400>  42
gatgttgttc cacccgcatc t                                                 21


<210>  43
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT2F2

<400>  43
cactgagcca agtcactgga                                                   20


<210>  44
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT2R2

<400>  44
agggacaaaa ccaaacagca                                                   20


<210>  45
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT3F1

<400>  45
tggccttcta aagcaaccaa                                                   20


<210>  46
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT3R1

<400>  46
ccgtctccca gatcacatga c                                                 21


<210>  47
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT3F2

<400>  47
ataactcata atctatgcat ggcacaa                                           27


<210>  48
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT3R2

<400>  48
tttgatcatg gtgtttatca gagc                                              24


<210>  49
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT4F1

<400>  49
gtccccgaca aaccaagcta                                                   20


<210>  50
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT4R1

<400>  50
tgaactggca gtatgcaggg                                                   20


<210>  51
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT4F2

<400>  51
aacatggttt ctgccctcag                                                   20


<210>  52
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT4R2

<400>  52
ggaccatgcc gaactcttac                                                   20


<210>  53
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT5F1

<400>  53
ttgagaccta gctcatgccc                                                   20


<210>  54
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT5R1

<400>  54
taacgcagaa ctggcacagg                                                   20


<210>  55
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT5F2

<400>  55
ctctcccatg gagaaagctg                                                   20


<210>  56
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT5R2

<400>  56
gaatgtggca ttggcttttt                                                   20


<210>  57
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT6F1

<400>  57
gagagagcca atctgcccat                                                   20


<210>  58
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT6R1

<400>  58
caccggaaac gtgtgagaga                                                   20


<210>  59
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT6F2

<400>  59
acttcccatg atcccattga                                                   20


<210>  60
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT6R2

<400>  60
agcttccctc caagtgtcct                                                   20


<210>  61
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT7F1

<400>  61
gatgggcata agcccgaagt a                                                 21


<210>  62
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT7R1

<400>  62
taaggcccag tgttgttgtg t                                                 21


<210>  63
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT7F2

<400>  63
gtagcagggg ctctgtgaag                                                   20


<210>  64
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT7R2

<400>  64
tggcctgaaa tacccagaac                                                   20


<210>  65
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT8F1

<400>  65
gttgaattcg cgtgtccagg                                                   20


<210>  66
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT8R1

<400>  66
tcccatggcg agaatgtcac                                                   20


<210>  67
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT8F2

<400>  67
ccctgtagga aacacagagg a                                                 21


<210>  68
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT8R2

<400>  68
tgctttggat gttgattcta aa                                                22


<210>  69
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT9F1

<400>  69
ggcaaaggac tagcttgcac                                                   20


<210>  70
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT9R1

<400>  70
gggtgctatg aggaccagtg                                                   20


<210>  71
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT9F2

<400>  71
cagtggtgtg tggagagctg                                                   20


<210>  72
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT9R2

<400>  72
agagagagcg cttgactttg a                                                 21


<210>  73
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT10F1

<400>  73
ctttgactcc cggcgaaaga                                                   20


<210>  74
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT10R1

<400>  74
ttgtccatcc gggtcattgc                                                   20


<210>  75
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT10F2

<400>  75
aagtccttct tgcccaactt                                                   20


<210>  76
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT10R2

<400>  76
cagccccaat gcattttt                                                     18


<210>  77
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT11F1

<400>  77
attacaggtc ctggttgggc                                                   20


<210>  78
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT11R1

<400>  78
actgagcctg gtagagcctt                                                   20


<210>  79
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT11F2

<400>  79
ggaaggtgaa ggaaggaagg                                                   20


<210>  80
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT11R2

<400>  80
ttttctagga attcaggaca taca                                              24


<210>  81
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT12F

<400>  81
tcatggtcct taaaattttt gc                                                22


<210>  82
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT12R

<400>  82
tccaggtatg caaagtggat                                                   20


<210>  83
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT13F1

<400>  83
ttcagttgta ctttggatgc tctga                                             25


<210>  84
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT13R1

<400>  84
catctgaata gcagcaggcg                                                   20


<210>  85
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT13F2

<400>  85
tagcacagcc caaatgactt                                                   20


<210>  86
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT13R2

<400>  86
tcatgaaacc ccataatcag aa                                                22


<210>  87
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT14F1

<400>  87
agtgggtcat cctttgttac cc                                                22


<210>  88
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT14R1

<400>  88
tgccagttat cagccaagca                                                   20


<210>  89
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT14F2

<400>  89
cccaggaact taactcaggt g                                                 21


<210>  90
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT14R2

<400>  90
tgccatttga cctcataagt ct                                                22


<210>  91
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT15F1

<400>  91
ttcagccccc ttgagtgttt a                                                 21


<210>  92
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT15R1

<400>  92
gtctctgagc acaaagagac ga                                                22


<210>  93
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT15F2

<400>  93
ttgcctgtcc caactagagc                                                   20


<210>  94
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT15R2

<400>  94
ggcccaagaa tgcacattta                                                   20


<210>  95
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT16F1

<400>  95
ccacacactg gctaggactg                                                   20


<210>  96
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT16R1

<400>  96
actggcagca cttgagacaa                                                   20


<210>  97
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT16F2

<400>  97
gatggcatgc tgtggttttt                                                   20


<210>  98
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OTC_OT16R2

<400>  98
caatgcttcc acacagaacc                                                   20


<210>  99
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA1

<400>  99
tctcttttaa actaacccat                                                   20


<210>  100
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT1

<400>  100
tatcttttca actaacccaa                                                   20


<210>  101
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT2

<400>  101
tttcttttat actagcccat                                                   20


<210>  102
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT3

<400>  102
tcttttttta aataacccat                                                   20


<210>  103
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT4

<400>  103
tggcatttaa actaacccaa                                                   20


<210>  104
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT5

<400>  104
tcttcatgaa actaacccat                                                   20


<210>  105
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT6

<400>  105
atttttttaa acaaacccat                                                   20


<210>  106
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT7

<400>  106
gatcttgtaa tctaacccat                                                   20


<210>  107
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT8

<400>  107
ccttttataa tctaacccat                                                   20


<210>  108
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT9

<400>  108
tctgttttaa agtaaccctt                                                   20


<210>  109
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT10

<400>  109
ttgcttttca actaacccaa                                                   20


<210>  110
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT11

<400>  110
aatattttaa actatcccat                                                   20


<210>  111
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT12

<400>  111
ttactttaaa actatcccat                                                   20


<210>  112
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT13

<400>  112
acacttttaa aataacccag                                                   20


<210>  113
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT14

<400>  113
aatcttctaa accaacccat                                                   20


<210>  114
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT15

<400>  114
tctttattag actaacccag                                                   20


<210>  115
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT16

<400>  115
tttcatttaa cataacccat                                                   20


<210>  116
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT17

<400>  116
tctttttaga agtaacccat                                                   20


<210>  117
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT18

<400>  117
tccctttttc actaacccac                                                   20


<210>  118
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT19

<400>  118
tctttgtaaa attaacccat                                                   20


<210>  119
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT20

<400>  119
tttattttaa agtaacccac                                                   20


<210>  120
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT21

<400>  120
tatctttcaa aataacccac                                                   20


<210>  121
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT22

<400>  121
tatcttggaa actaacccct                                                   20


<210>  122
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT23

<400>  122
cttcttttaa acaaacccag                                                   20


<210>  123
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT24

<400>  123
aatcttttat actaaccaat                                                   20


<210>  124
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT25

<400>  124
tattttttaa attaactcat                                                   20


<210>  125
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT26

<400>  125
tttattttaa aataacacat                                                   20


<210>  126
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT27

<400>  126
tctctttcaa actatcccaa                                                   20


<210>  127
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT28

<400>  127
tatctttaaa aataacacat                                                   20


<210>  128
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT29

<400>  128
tttctttaaa aataacacat                                                   20


<210>  129
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT30

<400>  129
gttcttttaa aaaaacccat                                                   20


<210>  130
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT31

<400>  130
tctgatttaa aataacacat                                                   20


<210>  131
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT32

<400>  131
cctcttataa cctaacccac                                                   20


<210>  132
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT33

<400>  132
cctcttgtaa gctaacccac                                                   20


<210>  133
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT34

<400>  133
cctattttaa agtaaccctt                                                   20


<210>  134
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT35

<400>  134
catcttgtaa actagcccat                                                   20


<210>  135
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT36

<400>  135
actcttttac aataacacat                                                   20


<210>  136
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT37

<400>  136
tctcctttag cctaactcat                                                   20


<210>  137
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT38

<400>  137
cctctttaaa aataacccct                                                   20


<210>  138
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT39

<400>  138
tatctttgaa ataaacccat                                                   20


<210>  139
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT40

<400>  139
tatatttaaa actaagccat                                                   20


<210>  140
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT41

<400>  140
tgactttaaa actaagccat                                                   20


<210>  141
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT42

<400>  141
cttcttttca actatcccat                                                   20


<210>  142
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT43

<400>  142
gctcttataa attaacccag                                                   20


<210>  143
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT44

<400>  143
tttcttttaa attaagccat                                                   20


<210>  144
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT45

<400>  144
tctctttgtc acaaacccat                                                   20


<210>  145
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT46

<400>  145
tttcttctaa gttaacccat                                                   20


<210>  146
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT47

<400>  146
tcacttccaa actaccccat                                                   20


<210>  147
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT48

<400>  147
tatcttataa aataacccag                                                   20


<210>  148
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OT49

<400>  148
tcaattttta actatcccat                                                   20


<210>  149
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dIDUA_sgRNA1_Fwd

<400>  149
caccgttctg atgagggctc cgcgg                                             25


<210>  150
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dIDUA_sgRNA1_Rev

<400>  150
aaacccgcgg agccctcatc agaac                                             25


<210>  151
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dIDUA_sgRNA2_Fwd

<400>  151
caccgcacct ggtgctcgtg gacg                                              24


<210>  152
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dlDUA_sgRNA2_Rev

<400>  152
aaaccgtcca cgagcaccag gtgc                                              24


<210>  153
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dIDUA_sgRNA3_Fwd

<400>  153
caccgggccg cgtccacgag cacc                                              24


<210>  154
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dlDUA_sgRNA3_Rev

<400>  154
aaacggtgct cgtggacgcg gccc                                              24


<210>  155
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dIDUA_sgRNA4_Fwd

<400>  155
caccgtccac gagcaccagg tgcg                                              24


<210>  156
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dlDUA_sgRNA4_Rev

<400>  156
aaaccgcacc tggtgctcgt ggac                                              24


<210>  157
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dIDUA_sgRNA5_Fwd

<400>  157
caccgtggac gcggcccgcg cgctg                                             25


<210>  158
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dlDUA_sgRNA5_Rev

<400>  158
aaaccagcgc gcgggccgcg tccac                                             25


<210>  159
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dIDUA_sgRNA6_Fwd

<400>  159
caccgcttct gatgagggct ccgc                                              24


<210>  160
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dlDUA_sgRNA6_Rev

<400>  160
aaacgcggag ccctcatcag aagc                                              24


<210>  161
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hCFTR_sgRNA1_Fwd

<400>  161
caccgaatgg tgccaggcat aatcc                                             25


<210>  162
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hCFTR_sgRNA1_Rev

<400>  162
aaacggatta tgcctggcac cattc                                             25


<210>  163
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hCFTR_sgRNA2_Fwd

<400>  163
caccgcaaag catgccaact agaag                                             25


<210>  164
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hCFTR_sgRNA2_Rev

<400>  164
aaaccttcta gttggcatgc tttgc                                             25


<210>  165
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hCFTR_sgRNA3_Fwd

<400>  165
caccgaccat taaagaaaat atcat                                             25


<210>  166
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hCFTR_sgRNA3_Rev

<400>  166
aaacatgata ttttctttaa tggtc                                             25


<210>  167
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hCFTR_sgRNA4_Fwd

<400>  167
caccgagttt cttacctctt ctagt                                             25


<210>  168
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hCFTR_sgRNA4_Rev

<400>  168
aaacactaga agaggtaaga aactc                                             25


<210>  169
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mFIXsgRNA1_Fwd

<400>  169
caccgtttag gatatctact cagta                                             25


<210>  170
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mFIXsgRNA1_Rev

<400>  170
aaactactga gtagatatcc taaac                                             25


<210>  171
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mFIXsgRNA2_Fwd

<400>  171
caccgacaaa cctgcacatt cggta                                             25


<210>  172
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mFIXsgRNA2_Rev

<400>  172
aaactaccga atgtgcaggt ttgtc                                             25


<210>  173
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mFIXsgRNA3_Fwd

<400>  173
caccgcacct gaacaccgtc atgg                                              24


<210>  174
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mFIXsgRNA3_Rev

<400>  174
aaacccatga cggtgttcag gtgc                                              24


<210>  175
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mFIX_PointM_F

<400>  175
cgagggagat ggacaacaat                                                   20


<210>  176
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mFIX_PointM_R

<400>  176
gcaccacaag ccctgtaaat                                                   20


<210>  177
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rhFIXsgRNA1_Fwd

<400>  177
caccgcgtgt gaacatgatc atgg                                              24


<210>  178
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rhFIXsgRNA1_Rev

<400>  178
aaacccatga tcatgttcac acgc                                              24


<210>  179
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rhFIXsgRNA2_Fwd

<400>  179
caccgtttag gatatctact cagtg                                             25


<210>  180
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rhFIXsgRNA2_Rev

<400>  180
aaaccactga gtagatatcc taaac                                             25


<210>  181
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rhFIXsgRNA3_Fwd

<400>  181
caccgacaaa cctgtacatt cagca                                             25


<210>  182
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rhFIXsgRNA3_Rev

<400>  182
aaactgctga atgtacaggt ttgtc                                             25


<210>  183
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EGFP-sgRNAF

<400>  183
caccgtcgtg ctgcttcatg tggt                                              24


<210>  184
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EGFP-sgRNAR

<400>  184
aaacaccaca tgaagcagca cgac                                              24


<210>  185
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCSK9_sgRNA1F

<400>  185
caccgatgct ctgggcaaag acag                                              24


<210>  186
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCSK9_sgRNA1R

<400>  186
aaacctgtct ttgcccagag catc                                              24


<210>  187
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCSK9_sgRNA2F

<400>  187
caccgatctc ctagacacca gcata                                             25


<210>  188
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCSK9_sgRNA2R

<400>  188
aaactatgct ggtgtctagg agatc                                             25


<210>  189
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCSK9_sgRNA3F

<400>  189
caccgaagtt ggtccccaaa gtccc                                             25


<210>  190
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCSK9_sgRNA3R

<400>  190
aaacgggact ttggggacca acttc                                             25


<210>  191
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCSK9_sgRNA4F

<400>  191
caccgcagca cctgctttgt gtca                                              24


<210>  192
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCSK9_sgRNA4R

<400>  192
aaactgacac aaagcaggtg ctgc                                              24


<210>  193
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCSK9_sgRNA5F

<400>  193
caccgagtct ctgcctcaac tcggc                                             25


<210>  194
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCSK9_sgRNA5R

<400>  194
aaacgccgag ttgaggcaga gactc                                             25


<210>  195
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCSK9_sgRNA6F

<400>  195
caccgatgac atctttggca gagaa                                             25


<210>  196
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCSK9_sgRNA6R

<400>  196
aaacttctct gccaaagatg tcatc                                             25


<210>  197
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCSK9_sgRNA7F

<400>  197
caccgcctgg ttccctgagg acca                                              24


<210>  198
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCSK9_sgRNA7R

<400>  198
aaactggtcc tcagggaacc aggc                                              24


