                         SEQUENCE LISTING

<110>  UCL Business PLC
 
<120>  CELL

<130>  P106930PCT

<150>  GB1507368.7
<151>  2015-04-30

<160>  27    

<170>  PatentIn version 3.5

<210>  1
<211>  560
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  aCD33-Fc-DAP10 CAR (chimeric antigen receptor)

<400>  1

Met Ala Val Pro Thr Gln Val Leu Gly Leu Leu Leu Leu Trp Leu Thr 
1               5                   10                  15      


Asp Ala Arg Cys Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser 
            20                  25                  30          


Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Glu Asp 
        35                  40                  45              


Ile Tyr Phe Asn Leu Val Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro 
    50                  55                  60                  


Lys Leu Leu Ile Tyr Asp Thr Asn Arg Leu Ala Asp Gly Val Pro Ser 
65                  70                  75                  80  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Gln Tyr Thr Leu Thr Ile Ser 
                85                  90                  95      


Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln His Tyr Lys 
            100                 105                 110         


Asn Tyr Pro Leu Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Arg Ser Glu Val Gln Leu Val Glu Ser Gly Gly 
145                 150                 155                 160 


Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser 
                165                 170                 175     


Gly Phe Thr Leu Ser Asn Tyr Gly Met His Trp Ile Arg Gln Ala Pro 
            180                 185                 190         


Gly Lys Gly Leu Glu Trp Val Ser Ser Ile Ser Leu Asn Gly Gly Ser 
        195                 200                 205             


Thr Tyr Tyr Arg Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp 
    210                 215                 220                 


Asn Ala Lys Ser Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu 
225                 230                 235                 240 


Asp Thr Ala Val Tyr Tyr Cys Ala Ala Gln Asp Ala Tyr Thr Gly Gly 
                245                 250                 255     


Tyr Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Met 
            260                 265                 270         


Asp Pro Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro 
        275                 280                 285             


Cys Pro Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro 
    290                 295                 300                 


Lys Pro Lys Asp Thr Leu Met Ile Ala Arg Thr Pro Glu Val Thr Cys 
305                 310                 315                 320 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
                325                 330                 335     


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
            340                 345                 350         


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
        355                 360                 365             


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
    370                 375                 380                 


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
385                 390                 395                 400 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
                405                 410                 415     


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
            420                 425                 430         


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
        435                 440                 445             


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
    450                 455                 460                 


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
465                 470                 475                 480 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
                485                 490                 495     


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Lys Asp Pro Lys Phe Trp 
            500                 505                 510         


Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val 
        515                 520                 525             


Thr Val Ala Phe Ile Ile Phe Trp Val Cys Ala Arg Pro Arg Arg Ser 
    530                 535                 540                 


Pro Ala Gln Glu Asp Gly Lys Val Tyr Ile Asn Met Pro Gly Arg Gly 
545                 550                 555                 560 


<210>  2
<211>  551
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  aGD2-Fc-DAP10 CAR

<400>  2

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val 
            20                  25                  30          


Lys Pro Ser Gln Thr Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser 
        35                  40                  45              


Leu Ala Ser Tyr Asn Ile His Trp Val Arg Gln Pro Pro Gly Lys Gly 
    50                  55                  60                  


Leu Glu Trp Leu Gly Val Ile Trp Ala Gly Gly Ser Thr Asn Tyr Asn 
65                  70                  75                  80  


Ser Ala Leu Met Ser Arg Leu Thr Ile Ser Lys Asp Asn Ser Lys Asn 
                85                  90                  95      


Gln Val Phe Leu Lys Met Ser Ser Leu Thr Ala Ala Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Lys Arg Ser Asp Asp Tyr Ser Trp Phe Ala Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Asn Gln Met Thr Gln Ser 
145                 150                 155                 160 


Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Met Thr Cys 
                165                 170                 175     


Arg Ala Ser Ser Ser Val Ser Ser Ser Tyr Leu His Trp Tyr Gln Gln 
            180                 185                 190         


Lys Ser Gly Lys Ala Pro Lys Val Trp Ile Tyr Ser Thr Ser Asn Leu 
        195                 200                 205             


Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Tyr Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr 
225                 230                 235                 240 


Tyr Cys Gln Gln Tyr Ser Gly Tyr Pro Ile Thr Phe Gly Gln Gly Thr 
                245                 250                 255     


Lys Val Glu Ile Lys Arg Ser Asp Pro Ala Glu Pro Lys Ser Pro Asp 
            260                 265                 270         


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro 
        275                 280                 285             


Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ala 
    290                 295                 300                 


Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp 
305                 310                 315                 320 


Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn 
                325                 330                 335     


Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val 
            340                 345                 350         


Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu 
        355                 360                 365             


Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys 
    370                 375                 380                 


Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr 
385                 390                 395                 400 


Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr 
                405                 410                 415     


Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
            420                 425                 430         


Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
        435                 440                 445             


Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys 
    450                 455                 460                 


Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu 
465                 470                 475                 480 


Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
                485                 490                 495     


Lys Lys Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly Val Leu 
            500                 505                 510         


Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
        515                 520                 525             


Cys Ala Arg Pro Arg Arg Ser Pro Ala Gln Glu Asp Gly Lys Val Tyr 
    530                 535                 540                 


Ile Asn Met Pro Gly Arg Gly 
545                 550     


<210>  3
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DAP10 signalling domain

<400>  3

Cys Ala Arg Pro Arg Arg Ser Pro Ala Gln Glu Asp Gly Lys Val Tyr 
1               5                   10                  15      


Ile Asn Met Pro Gly Arg Gly 
            20              


<210>  4
<211>  1683
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleic acid sequence which encodes a CAR, aCD33-Fc-DAP10 CAR

<400>  4
atggccgtgc ccactcaggt cctggggttg ttgctactgt ggcttacaga tgccagatgt       60

gacatccaga tgacacagtc tccatcttcc ctgtctgcat ctgtcggaga tcgcgtcacc      120

atcacctgtc gagcaagtga ggacatttat tttaatttag tgtggtatca gcagaaacca      180

ggaaaggccc ctaagctcct gatctatgat acaaatcgct tggcagatgg ggtcccatca      240

cggttcagtg gctctggatc tggcacacag tatactctaa ccataagtag cctgcaaccc      300

gaagatttcg caacctatta ttgtcaacac tataagaatt atccgctcac gttcggtcag      360

gggaccaagc tggaaatcaa aagatctggt ggcggagggt caggaggcgg aggcagcgga      420

ggcggtggct cgggaggcgg aggctcgaga tctgaggtgc agttggtgga gtctgggggc      480

ggcttggtgc agcctggagg gtccctgagg ctctcctgtg cagcctcagg attcactctc      540

agtaattatg gcatgcactg gatcaggcag gctccaggga agggtctgga gtgggtctcg      600

tctattagtc ttaatggtgg tagcacttac tatcgagact ccgtgaaggg ccgattcact      660

atctccaggg acaatgcaaa aagcaccctc taccttcaaa tgaatagtct gagggccgag      720

gacacggccg tctattactg tgcagcacag gacgcttata cgggaggtta ctttgattac      780

tggggccaag gaacgctggt cacagtctcg tctatggatc ccgccgagcc caaatctcct      840

gacaaaactc acacatgccc accgtgccca gcacctcccg tggccggccc gtcagtcttc      900

ctcttccccc caaaacccaa ggacaccctc atgatcgccc ggacccctga ggtcacatgc      960

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc     1020

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt     1080

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc     1140

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg     1200

cagccccgag aaccacaggt gtacaccctg cccccatccc gggatgagct gaccaagaac     1260

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg     1320

gagagcaatg ggcaaccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac     1380

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac     1440

gtcttctcat gctccgtgat gcatgaggcc ctgcacaatc actataccca gaaatctctg     1500

agtctgagcc caggcaagaa ggaccccaag ttctgggtcc tggtggtggt gggaggcgtg     1560

ctggcctgtt actctctcct ggtgaccgtg gccttcatca tcttctgggt gtgcgccaga     1620

ccacggcgga gcccagccca ggaggacggc aaggtgtaca tcaacatgcc cggccgcggc     1680

tga                                                                   1683


<210>  5
<211>  1656
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleic acid sequence which encodes a CAR, aGD2-Fc-DAP10 CAR

<400>  5
atggagaccg acaccctgct gctgtgggtg ctgctgctgt gggtgccagg cagcaccggc       60

caggtgcagc tgcaggagtc tggcccaggc ctggtgaagc ccagccagac cctgagcatc      120

acctgcaccg tgagcggctt cagcctggcc agctacaaca tccactgggt gcggcagccc      180

ccaggcaagg gcctggagtg gctgggcgtg atctgggctg gcggcagcac caactacaac      240

agcgccctga tgagccggct gaccatcagc aaggacaaca gcaagaacca ggtgttcctg      300

aagatgagca gcctgacagc cgccgacacc gccgtgtact actgcgccaa gcggagcgac      360

gactacagct ggttcgccta ctggggccag ggcaccctgg tgaccgtgag ctctggcgga      420

ggcggctctg gcggaggcgg ctctggcgga ggcggcagcg agaaccagat gacccagagc      480

cccagcagct tgagcgccag cgtgggcgac cgggtgacca tgacctgcag agccagcagc      540

agcgtgagca gcagctacct gcactggtac cagcagaaga gcggcaaggc cccaaaggtg      600

tggatctaca gcaccagcaa cctggccagc ggcgtgccca gccggttcag cggcagcggc      660

agcggcaccg actacaccct gaccatcagc agcctgcagc ccgaggactt cgccacctac      720

tactgccagc agtacagcgg ctaccccatc accttcggcc agggcaccaa ggtggagatc      780

aagcggtcgg atcccgccga gcccaaatct cctgacaaaa ctcacacatg cccaccgtgc      840

ccagcacctc ccgtggccgg cccgtcagtc ttcctcttcc ccccaaaacc caaggacacc      900

ctcatgatcg cccggacccc tgaggtcaca tgcgtggtgg tggacgtgag ccacgaagac      960

cctgaggtca agttcaactg gtacgtggac ggcgtggagg tgcataatgc caagacaaag     1020

ccgcgggagg agcagtacaa cagcacgtac cgtgtggtca gcgtcctcac cgtcctgcac     1080

caggactggc tgaatggcaa ggagtacaag tgcaaggtct ccaacaaagc cctcccagcc     1140

cccatcgaga aaaccatctc caaagccaaa gggcagcccc gagaaccaca ggtgtacacc     1200

ctgcccccat cccgggatga gctgaccaag aaccaggtca gcctgacctg cctggtcaaa     1260

ggcttctatc ccagcgacat cgccgtggag tgggagagca atgggcaacc ggagaacaac     1320

tacaagacca cgcctcccgt gctggactcc gacggctcct tcttcctcta cagcaagctc     1380

accgtggaca agagcaggtg gcagcagggg aacgtcttct catgctccgt gatgcatgag     1440

gccctgcaca atcactatac ccagaaatct ctgagtctga gcccaggcaa gaaggacccc     1500

aagttctggg tcctggtggt ggtgggaggc gtgctggcct gttactctct cctggtgacc     1560

gtggccttca tcatcttctg ggtgtgcgcc agaccacggc ggagcccagc ccaggaggac     1620

ggcaaggtgt acatcaacat gcccggccgc ggctga                               1656


<210>  6
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signal peptide

<400>  6

Met Gly Thr Ser Leu Leu Cys Trp Met Ala Leu Cys Leu Leu Gly Ala 
1               5                   10                  15      


Asp His Ala Asp Gly 
            20      


<210>  7
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signal peptide derived from IgG1

<400>  7

Met Ser Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


<210>  8
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signal peptide derived from CD8

<400>  8

Met Ala Val Pro Thr Gln Val Leu Gly Leu Leu Leu Leu Trp Leu Thr 
1               5                   10                  15      


Asp Ala Arg Cys 
            20  


<210>  9
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  co-stimulatory domain, CD28 endodomain

<400>  9

Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg 
1               5                   10                  15      


Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg 
            20                  25                  30          


Asp Phe Ala Ala Tyr 
        35          


<210>  10
<211>  48
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  co-stimulatory domain, CD27 endodomain

<400>  10

Gln Arg Arg Lys Tyr Arg Ser Asn Lys Gly Glu Ser Pro Val Glu Pro 
1               5                   10                  15      


Ala Glu Pro Cys His Tyr Ser Cys Pro Arg Glu Glu Glu Gly Ser Thr 
            20                  25                  30          


Ile Pro Ile Gln Glu Asp Tyr Arg Lys Pro Glu Pro Ala Cys Ser Pro 
        35                  40                  45              


<210>  11
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  co-stimulatory domain, 41BB endodomain

<400>  11

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210>  12
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  co-stimulatory domain, OX40 endodomain

<400>  12

Arg Arg Asp Gln Arg Leu Pro Pro Asp Ala His Lys Pro Pro Gly Gly 
1               5                   10                  15      


Gly Ser Phe Arg Thr Pro Ile Gln Glu Glu Gln Ala Asp Ala His Ser 
            20                  25                  30          


Thr Leu Ala Lys Ile 
        35          


<210>  13
<211>  188
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  co-stimulatory domain, CD30 endodomain

<400>  13

His Arg Arg Ala Cys Arg Lys Arg Ile Arg Gln Lys Leu His Leu Cys 
1               5                   10                  15      


Tyr Pro Val Gln Thr Ser Gln Pro Lys Leu Glu Leu Val Asp Ser Arg 
            20                  25                  30          


Pro Arg Arg Ser Ser Thr Gln Leu Arg Ser Gly Ala Ser Val Thr Glu 
        35                  40                  45              


Pro Val Ala Glu Glu Arg Gly Leu Met Ser Gln Pro Leu Met Glu Thr 
    50                  55                  60                  


Cys His Ser Val Gly Ala Ala Tyr Leu Glu Ser Leu Pro Leu Gln Asp 
65                  70                  75                  80  


Ala Ser Pro Ala Gly Gly Pro Ser Ser Pro Arg Asp Leu Pro Glu Pro 
                85                  90                  95      


Arg Val Ser Thr Glu His Thr Asn Asn Lys Ile Glu Lys Ile Tyr Ile 
            100                 105                 110         


Met Lys Ala Asp Thr Val Ile Val Gly Thr Val Lys Ala Glu Leu Pro 
        115                 120                 125             


Glu Gly Arg Gly Leu Ala Gly Pro Ala Glu Pro Glu Leu Glu Glu Glu 
    130                 135                 140                 


Leu Glu Ala Asp His Thr Pro His Tyr Pro Glu Gln Glu Thr Glu Pro 
145                 150                 155                 160 


Pro Leu Gly Ser Cys Ser Asp Val Met Leu Ser Val Glu Glu Glu Gly 
                165                 170                 175     


Lys Glu Asp Pro Leu Pro Thr Ala Ala Ser Gly Lys 
            180                 185             


<210>  14
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  co-stimulatory domain, IL2-R endodomain

<400>  14

Thr Trp Gln Arg Arg Gln Arg Lys Ser Arg Arg Thr Ile 
1               5                   10              


<210>  15
<211>  195
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  co-stimulatory domain, IL7-R endodomain

<400>  15

Lys Lys Arg Ile Lys Pro Ile Val Trp Pro Ser Leu Pro Asp His Lys 
1               5                   10                  15      


Lys Thr Leu Glu His Leu Cys Lys Lys Pro Arg Lys Asn Leu Asn Val 
            20                  25                  30          


Ser Phe Asn Pro Glu Ser Phe Leu Asp Cys Gln Ile His Arg Val Asp 
        35                  40                  45              


Asp Ile Gln Ala Arg Asp Glu Val Glu Gly Phe Leu Gln Asp Thr Phe 
    50                  55                  60                  


Pro Gln Gln Leu Glu Glu Ser Glu Lys Gln Arg Leu Gly Gly Asp Val 
65                  70                  75                  80  


Gln Ser Pro Asn Cys Pro Ser Glu Asp Val Val Ile Thr Pro Glu Ser 
                85                  90                  95      


Phe Gly Arg Asp Ser Ser Leu Thr Cys Leu Ala Gly Asn Val Ser Ala 
            100                 105                 110         


Cys Asp Ala Pro Ile Leu Ser Ser Ser Arg Ser Leu Asp Cys Arg Glu 
        115                 120                 125             


Ser Gly Lys Asn Gly Pro His Val Tyr Gln Asp Leu Leu Leu Ser Leu 
    130                 135                 140                 


Gly Thr Thr Asn Ser Thr Leu Pro Pro Pro Phe Ser Leu Gln Ser Gly 
145                 150                 155                 160 


Ile Leu Thr Leu Asn Pro Val Ala Gln Gly Gln Pro Ile Leu Thr Ser 
                165                 170                 175     


Leu Gly Ser Asn Gln Glu Glu Ala Tyr Val Thr Met Ser Ser Phe Tyr 
            180                 185                 190         


Gln Asn Gln 
        195 


<210>  16
<211>  285
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  co-stimulatory domain, IL21-R endodomain

<400>  16

Ser Leu Lys Thr His Pro Leu Trp Arg Leu Trp Lys Lys Ile Trp Ala 
1               5                   10                  15      


Val Pro Ser Pro Glu Arg Phe Phe Met Pro Leu Tyr Lys Gly Cys Ser 
            20                  25                  30          


Gly Asp Phe Lys Lys Trp Val Gly Ala Pro Phe Thr Gly Ser Ser Leu 
        35                  40                  45              


Glu Leu Gly Pro Trp Ser Pro Glu Val Pro Ser Thr Leu Glu Val Tyr 
    50                  55                  60                  


Ser Cys His Pro Pro Arg Ser Pro Ala Lys Arg Leu Gln Leu Thr Glu 
65                  70                  75                  80  


Leu Gln Glu Pro Ala Glu Leu Val Glu Ser Asp Gly Val Pro Lys Pro 
                85                  90                  95      


Ser Phe Trp Pro Thr Ala Gln Asn Ser Gly Gly Ser Ala Tyr Ser Glu 
            100                 105                 110         


Glu Arg Asp Arg Pro Tyr Gly Leu Val Ser Ile Asp Thr Val Thr Val 
        115                 120                 125             


Leu Asp Ala Glu Gly Pro Cys Thr Trp Pro Cys Ser Cys Glu Asp Asp 
    130                 135                 140                 


Gly Tyr Pro Ala Leu Asp Leu Asp Ala Gly Leu Glu Pro Ser Pro Gly 
145                 150                 155                 160 


Leu Glu Asp Pro Leu Leu Asp Ala Gly Thr Thr Val Leu Ser Cys Gly 
                165                 170                 175     


Cys Val Ser Ala Gly Ser Pro Gly Leu Gly Gly Pro Leu Gly Ser Leu 
            180                 185                 190         


Leu Asp Arg Leu Lys Pro Pro Leu Ala Asp Gly Glu Asp Trp Ala Gly 
        195                 200                 205             


Gly Leu Pro Trp Gly Gly Arg Ser Pro Gly Gly Val Ser Glu Ser Glu 
    210                 215                 220                 


Ala Gly Ser Pro Leu Ala Gly Leu Asp Met Asp Thr Phe Asp Ser Gly 
225                 230                 235                 240 


Phe Val Gly Ser Asp Cys Ser Ser Pro Val Glu Cys Asp Phe Thr Ser 
                245                 250                 255     


Pro Gly Asp Glu Gly Pro Pro Arg Ser Tyr Leu Arg Gln Trp Val Val 
            260                 265                 270         


Ile Pro Pro Pro Leu Ser Ser Pro Gly Pro Gln Ala Ser 
        275                 280                 285 


<210>  17
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  co-stimulatory domain, NKp30 endodomain

<400>  17

Gly Ser Thr Val Tyr Tyr Gln Gly Lys Cys Leu Thr Trp Lys Gly Pro 
1               5                   10                  15      


Arg Arg Gln Leu Pro Ala Val Val Pro Ala Pro Leu Pro Pro Pro Cys 
            20                  25                  30          


Gly Ser Ser Ala His Leu Leu Pro Pro Val Pro Gly Gly 
        35                  40                  45  


<210>  18
<211>  63
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  co-stimulatory domain, NKp44 endodomain

<400>  18

Trp Trp Gly Asp Ile Trp Trp Lys Thr Met Met Glu Leu Arg Ser Leu 
1               5                   10                  15      


Asp Thr Gln Lys Ala Thr Cys His Leu Gln Gln Val Thr Asp Leu Pro 
            20                  25                  30          


Trp Thr Ser Val Ser Ser Pro Val Glu Arg Glu Ile Leu Tyr His Thr 
        35                  40                  45              


Val Ala Arg Thr Lys Ile Ser Asp Asp Asp Asp Glu His Thr Leu 
    50                  55                  60              


<210>  19
<211>  61
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  co-stimulatory domain, DNAM-1 (CD226) endodomain

<400>  19

Asn Arg Arg Arg Arg Arg Glu Arg Arg Asp Leu Phe Thr Glu Ser Trp 
1               5                   10                  15      


Asp Thr Gln Lys Ala Pro Asn Asn Tyr Arg Ser Pro Ile Ser Thr Ser 
            20                  25                  30          


Gln Pro Thr Asn Gln Ser Met Asp Asp Thr Arg Glu Asp Ile Tyr Val 
        35                  40                  45              


Asn Tyr Pro Thr Phe Ser Arg Arg Pro Lys Thr Arg Val 
    50                  55                  60      


<210>  20
<211>  263
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  antigen binding domain

<400>  20

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val 
            20                  25                  30          


Lys Pro Ser Gln Thr Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser 
        35                  40                  45              


Leu Ala Ser Tyr Asn Ile His Trp Val Arg Gln Pro Pro Gly Lys Gly 
    50                  55                  60                  


Leu Glu Trp Leu Gly Val Ile Trp Ala Gly Gly Ser Thr Asn Tyr Asn 
65                  70                  75                  80  


Ser Ala Leu Met Ser Arg Leu Thr Ile Ser Lys Asp Asn Ser Lys Asn 
                85                  90                  95      


Gln Val Phe Leu Lys Met Ser Ser Leu Thr Ala Ala Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Lys Arg Ser Asp Asp Tyr Ser Trp Phe Ala Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Asn Gln Met Thr Gln Ser 
145                 150                 155                 160 


Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Met Thr Cys 
                165                 170                 175     


Arg Ala Ser Ser Ser Val Ser Ser Ser Tyr Leu His Trp Tyr Gln Gln 
            180                 185                 190         


Lys Ser Gly Lys Ala Pro Lys Val Trp Ile Tyr Ser Thr Ser Asn Leu 
        195                 200                 205             


Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Tyr Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr 
225                 230                 235                 240 


Tyr Cys Gln Gln Tyr Ser Gly Tyr Pro Ile Thr Phe Gly Gln Gly Thr 
                245                 250                 255     


Lys Val Glu Ile Lys Arg Ser 
            260             


<210>  21
<211>  272
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  antigen binding domain

<400>  21

Met Ala Val Pro Thr Gln Val Leu Gly Leu Leu Leu Leu Trp Leu Thr 
1               5                   10                  15      


Asp Ala Arg Cys Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser 
            20                  25                  30          


Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Glu Asp 
        35                  40                  45              


Ile Tyr Phe Asn Leu Val Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro 
    50                  55                  60                  


Lys Leu Leu Ile Tyr Asp Thr Asn Arg Leu Ala Asp Gly Val Pro Ser 
65                  70                  75                  80  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Gln Tyr Thr Leu Thr Ile Ser 
                85                  90                  95      


Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln His Tyr Lys 
            100                 105                 110         


Asn Tyr Pro Leu Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Arg Ser Glu Val Gln Leu Val Glu Ser Gly Gly 
145                 150                 155                 160 


Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser 
                165                 170                 175     


Gly Phe Thr Leu Ser Asn Tyr Gly Met His Trp Ile Arg Gln Ala Pro 
            180                 185                 190         


Gly Lys Gly Leu Glu Trp Val Ser Ser Ile Ser Leu Asn Gly Gly Ser 
        195                 200                 205             


Thr Tyr Tyr Arg Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp 
    210                 215                 220                 


Asn Ala Lys Ser Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu 
225                 230                 235                 240 


Asp Thr Ala Val Tyr Tyr Cys Ala Ala Gln Asp Ala Tyr Thr Gly Gly 
                245                 250                 255     


Tyr Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Met 
            260                 265                 270         


<210>  22
<211>  234
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence, hinge-CH2CH3 of human IgG1

<400>  22

Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15      


Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          


Lys Asp Thr Leu Met Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
    130                 135                 140                 


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            180                 185                 190         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                 200                 205             


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                 215                 220                 


Ser Leu Ser Leu Ser Pro Gly Lys Lys Asp 
225                 230                 


<210>  23
<211>  46
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence, human CD8 stalk

<400>  23

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile 
        35                  40                  45      


<210>  24
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence, human IgG1 hinge

<400>  24

Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15      


Lys Asp Pro Lys 
            20  


<210>  25
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 transmembrane domain

<400>  25

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25          


<210>  26
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3 zeta endodomain

<400>  26

Arg Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln 
1               5                   10                  15      


Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu 
            20                  25                  30          


Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly 
        35                  40                  45              


Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu 
    50                  55                  60                  


Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly 
65                  70                  75                  80  


Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser 
                85                  90                  95      


Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro 
            100                 105                 110         


Pro Arg 
        


<210>  27
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  activation motif


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  27

Tyr Xaa Xaa Met 
1               


