                         SEQUENCE LISTING

<110>  UCL Business PLC
 
<120>  NUCLEIC ACID CONSTRUCT

<130>  P106291PCT

<150>  GB1507111.1
<151>  2015-04-27

<160>  233   

<170>  PatentIn version 3.5

<210>  1
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tyrosinase-related protein (TYRP)-1 intracellular retention 
       signal

<400>  1

Asn Gln Pro Leu Leu Thr Asp 
1               5           


<210>  2
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Adenoviral E3/19K intracellular retention signal

<400>  2

Lys Tyr Lys Ser Arg Arg Ser Phe Ile Asp Glu Lys Lys Met Pro 
1               5                   10                  15  


<210>  3
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Adenoviral E3/19K intracellular retention signal

<400>  3

Asp Glu Lys Lys Met Pro 
1               5       


<210>  4
<211>  1129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR OR gate which recognizes CD19 OR CD33

<400>  4

Met Ser Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Lys Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Glu Val Lys Leu Gln Glu Ser Gly Pro Gly 
145                 150                 155                 160 


Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly 
                165                 170                 175     


Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg 
            180                 185                 190         


Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr 
        195                 200                 205             


Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser 
    210                 215                 220                 


Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr 
225                 230                 235                 240 


Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala 
                245                 250                 255     


Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Asp Pro 
            260                 265                 270         


Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
        275                 280                 285             


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
    290                 295                 300                 


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Phe Trp 
305                 310                 315                 320 


Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val 
                325                 330                 335     


Thr Val Ala Phe Ile Ile Phe Trp Val Arg Arg Val Lys Phe Ser Arg 
            340                 345                 350         


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
        355                 360                 365             


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
    370                 375                 380                 


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
385                 390                 395                 400 


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
                405                 410                 415     


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
            420                 425                 430         


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
        435                 440                 445             


Ala Leu His Met Gln Ala Leu Pro Pro Arg Arg Ala Glu Gly Arg Gly 
    450                 455                 460                 


Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Ala 
465                 470                 475                 480 


Val Pro Thr Gln Val Leu Gly Leu Leu Leu Leu Trp Leu Thr Asp Ala 
                485                 490                 495     


Arg Cys Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser 
            500                 505                 510         


Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Glu Asp Ile Tyr 
        515                 520                 525             


Phe Asn Leu Val Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu 
    530                 535                 540                 


Leu Ile Tyr Asp Thr Asn Arg Leu Ala Asp Gly Val Pro Ser Arg Phe 
545                 550                 555                 560 


Ser Gly Ser Gly Ser Gly Thr Gln Tyr Thr Leu Thr Ile Ser Ser Leu 
                565                 570                 575     


Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln His Tyr Lys Asn Tyr 
            580                 585                 590         


Pro Leu Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Ser Gly 
        595                 600                 605             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    610                 615                 620                 


Gly Gly Ser Arg Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu 
625                 630                 635                 640 


Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe 
                645                 650                 655     


Thr Leu Ser Asn Tyr Gly Met His Trp Ile Arg Gln Ala Pro Gly Lys 
            660                 665                 670         


Gly Leu Glu Trp Val Ser Ser Ile Ser Leu Asn Gly Gly Ser Thr Tyr 
        675                 680                 685             


Tyr Arg Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala 
    690                 695                 700                 


Lys Ser Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr 
705                 710                 715                 720 


Ala Val Tyr Tyr Cys Ala Ala Gln Asp Ala Tyr Thr Gly Gly Tyr Phe 
                725                 730                 735     


Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Met Asp Pro 
            740                 745                 750         


Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
        755                 760                 765             


Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
    770                 775                 780                 


Lys Asp Thr Leu Met Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val 
785                 790                 795                 800 


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
                805                 810                 815     


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
            820                 825                 830         


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
        835                 840                 845             


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
    850                 855                 860                 


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
865                 870                 875                 880 


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
                885                 890                 895     


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
            900                 905                 910         


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
        915                 920                 925             


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
    930                 935                 940                 


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
945                 950                 955                 960 


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
                965                 970                 975     


Ser Leu Ser Leu Ser Pro Gly Lys Lys Asp Pro Lys Phe Trp Val Leu 
            980                 985                 990         


Val Val Val Gly Gly Val Leu Ala  Cys Tyr Ser Leu Leu  Val Thr Val 
        995                 1000                 1005             


Ala Phe  Ile Ile Phe Trp Val  Arg Ser Arg Val Lys  Phe Ser Arg 
    1010                 1015                 1020             


Ser Ala  Asp Ala Pro Ala Tyr  Gln Gln Gly Gln Asn  Gln Leu Tyr 
    1025                 1030                 1035             


Asn Glu  Leu Asn Leu Gly Arg  Arg Glu Glu Tyr Asp  Val Leu Asp 
    1040                 1045                 1050             


Lys Arg  Arg Gly Arg Asp Pro  Glu Met Gly Gly Lys  Pro Arg Arg 
    1055                 1060                 1065             


Lys Asn  Pro Gln Glu Gly Leu  Tyr Asn Glu Leu Gln  Lys Asp Lys 
    1070                 1075                 1080             


Met Ala  Glu Ala Tyr Ser Glu  Ile Gly Met Lys Gly  Glu Arg Arg 
    1085                 1090                 1095             


Arg Gly  Lys Gly His Asp Gly  Leu Tyr Gln Gly Leu  Ser Thr Ala 
    1100                 1105                 1110             


Thr Lys  Asp Thr Tyr Asp Ala  Leu His Met Gln Ala  Leu Pro Pro 
    1115                 1120                 1125             


Arg 
    


<210>  5
<211>  234
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence, hinge-CH2CH3 of human IgG1

<400>  5

Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15      


Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          


Lys Asp Thr Leu Met Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
    130                 135                 140                 


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            180                 185                 190         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                 200                 205             


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                 215                 220                 


Ser Leu Ser Leu Ser Pro Gly Lys Lys Asp 
225                 230                 


<210>  6
<211>  46
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence, human CD8 stalk

<400>  6

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile 
        35                  40                  45      


<210>  7
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence, human IgG1 hinge

<400>  7

Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15      


Lys Asp Pro Lys 
            20  


<210>  8
<211>  185
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence, CD2 ectodomain

<400>  8

Lys Glu Ile Thr Asn Ala Leu Glu Thr Trp Gly Ala Leu Gly Gln Asp 
1               5                   10                  15      


Ile Asn Leu Asp Ile Pro Ser Phe Gln Met Ser Asp Asp Ile Asp Asp 
            20                  25                  30          


Ile Lys Trp Glu Lys Thr Ser Asp Lys Lys Lys Ile Ala Gln Phe Arg 
        35                  40                  45              


Lys Glu Lys Glu Thr Phe Lys Glu Lys Asp Thr Tyr Lys Leu Phe Lys 
    50                  55                  60                  


Asn Gly Thr Leu Lys Ile Lys His Leu Lys Thr Asp Asp Gln Asp Ile 
65                  70                  75                  80  


Tyr Lys Val Ser Ile Tyr Asp Thr Lys Gly Lys Asn Val Leu Glu Lys 
                85                  90                  95      


Ile Phe Asp Leu Lys Ile Gln Glu Arg Val Ser Lys Pro Lys Ile Ser 
            100                 105                 110         


Trp Thr Cys Ile Asn Thr Thr Leu Thr Cys Glu Val Met Asn Gly Thr 
        115                 120                 125             


Asp Pro Glu Leu Asn Leu Tyr Gln Asp Gly Lys His Leu Lys Leu Ser 
    130                 135                 140                 


Gln Arg Val Ile Thr His Lys Trp Thr Thr Ser Leu Ser Ala Lys Phe 
145                 150                 155                 160 


Lys Cys Thr Ala Gly Asn Lys Val Ser Lys Glu Ser Ser Val Glu Pro 
                165                 170                 175     


Val Ser Cys Pro Glu Lys Gly Leu Asp 
            180                 185 


<210>  9
<211>  259
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence, CD34 ectodomain

<400>  9

Ser Leu Asp Asn Asn Gly Thr Ala Thr Pro Glu Leu Pro Thr Gln Gly 
1               5                   10                  15      


Thr Phe Ser Asn Val Ser Thr Asn Val Ser Tyr Gln Glu Thr Thr Thr 
            20                  25                  30          


Pro Ser Thr Leu Gly Ser Thr Ser Leu His Pro Val Ser Gln His Gly 
        35                  40                  45              


Asn Glu Ala Thr Thr Asn Ile Thr Glu Thr Thr Val Lys Phe Thr Ser 
    50                  55                  60                  


Thr Ser Val Ile Thr Ser Val Tyr Gly Asn Thr Asn Ser Ser Val Gln 
65                  70                  75                  80  


Ser Gln Thr Ser Val Ile Ser Thr Val Phe Thr Thr Pro Ala Asn Val 
                85                  90                  95      


Ser Thr Pro Glu Thr Thr Leu Lys Pro Ser Leu Ser Pro Gly Asn Val 
            100                 105                 110         


Ser Asp Leu Ser Thr Thr Ser Thr Ser Leu Ala Thr Ser Pro Thr Lys 
        115                 120                 125             


Pro Tyr Thr Ser Ser Ser Pro Ile Leu Ser Asp Ile Lys Ala Glu Ile 
    130                 135                 140                 


Lys Cys Ser Gly Ile Arg Glu Val Lys Leu Thr Gln Gly Ile Cys Leu 
145                 150                 155                 160 


Glu Gln Asn Lys Thr Ser Ser Cys Ala Glu Phe Lys Lys Asp Arg Gly 
                165                 170                 175     


Glu Gly Leu Ala Arg Val Leu Cys Gly Glu Glu Gln Ala Asp Ala Asp 
            180                 185                 190         


Ala Gly Ala Gln Val Cys Ser Leu Leu Leu Ala Gln Ser Glu Val Arg 
        195                 200                 205             


Pro Gln Cys Leu Leu Leu Val Leu Ala Asn Arg Thr Glu Ile Ser Ser 
    210                 215                 220                 


Lys Leu Gln Leu Met Lys Lys His Gln Ser Asp Leu Lys Lys Leu Gly 
225                 230                 235                 240 


Ile Leu Asp Phe Thr Glu Gln Asp Val Ala Ser His Gln Ser Tyr Ser 
                245                 250                 255     


Gln Lys Thr 
            


<210>  10
<211>  140
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  comprising CD28 transmembrane domain and CD3 Z endodomain

<400>  10

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Arg Val Lys Phe 
            20                  25                  30          


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu 
        35                  40                  45              


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
    50                  55                  60                  


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys 
65                  70                  75                  80  


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
                85                  90                  95      


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
            100                 105                 110         


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
        115                 120                 125             


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
    130                 135                 140 


<210>  11
<211>  180
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  comprising CD28 transmembrane domain and CD28 and CD3 Zeta 
       endodomains

<400>  11

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser 
            20                  25                  30          


Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly 
        35                  40                  45              


Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala 
    50                  55                  60                  


Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala 
65                  70                  75                  80  


Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg 
                85                  90                  95      


Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu 
            100                 105                 110         


Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn 
        115                 120                 125             


Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
    130                 135                 140                 


Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly 
145                 150                 155                 160 


Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala 
                165                 170                 175     


Leu Pro Pro Arg 
            180 


<210>  12
<211>  216
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  comprising CD28 transmembrane domain and CD28, OX40 and CD3 Zeta 
       endodomains

<400>  12

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser 
            20                  25                  30          


Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly 
        35                  40                  45              


Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala 
    50                  55                  60                  


Ala Tyr Arg Ser Arg Asp Gln Arg Leu Pro Pro Asp Ala His Lys Pro 
65                  70                  75                  80  


Pro Gly Gly Gly Ser Phe Arg Thr Pro Ile Gln Glu Glu Gln Ala Asp 
                85                  90                  95      


Ala His Ser Thr Leu Ala Lys Ile Arg Val Lys Phe Ser Arg Ser Ala 
            100                 105                 110         


Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
        115                 120                 125             


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
    130                 135                 140                 


Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu 
145                 150                 155                 160 


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
                165                 170                 175     


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
            180                 185                 190         


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
        195                 200                 205             


His Met Gln Ala Leu Pro Pro Arg 
    210                 215     


<210>  13
<211>  3390
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MP13974.SFG.aCD19fmc63-CD8STK-CD28tmZ-2A-aCD33glx-HCH2CH3pvaa-CD2
       8tmZw

<400>  13
atgagcctgc ccgtgaccgc cctgctgctg cccctggccc tgctgctgca cgccgccaga       60

ccagacatcc agatgaccca gaccaccagc agcctgagcg ccagcctggg cgaccgggtg      120

accatcagct gcagagccag ccaggacatc agcaagtacc tgaactggta ccagcagaag      180

cccgacggca ccgtgaagct gctgatctac cacaccagcc ggctgcacag cggcgtgccc      240

agccggttca gcggcagcgg cagcggcacc gactacagcc tgaccatcag caacctggag      300

caggaggaca tcgccaccta cttctgccag cagggcaaca ccctgcccta caccttcgga      360

ggcggcacca agctggagat caccaaggcc ggaggcggag gctctggcgg aggcggctct      420

ggcggaggcg gctctggcgg aggcggcagc gaggtgaagc tgcaggagtc tggcccaggc      480

ctggtggccc caagccagag cctgagcgtg acctgcaccg tgagcggcgt gagcctgccc      540

gactacggcg tgagctggat caggcagccc ccacggaagg gcctggagtg gctgggcgtg      600

atctggggca gcgagaccac ctactacaac agcgccctga agagccggct gaccatcatc      660

aaggacaaca gcaagagcca ggtgttcctg aagatgaaca gcctgcagac cgacgacacc      720

gccatctact actgcgccaa gcactactac tatggcggca gctacgctat ggactactgg      780

ggccagggca ccagcgtgac cgtgagctca gatcccacca cgacgccagc gccgcgacca      840

ccaacaccgg cgcccaccat cgcgtcgcag cccctgtccc tgcgcccaga ggcgtgccgg      900

ccagcggcgg ggggcgcagt gcacacgagg gggctggact tcgcctgtga tatcttttgg      960

gtgctggtgg tggttggtgg agtcctggct tgctatagct tgctagtaac agtggccttt     1020

attattttct gggtgaggag agtgaagttc agcaggagcg cagacgcccc cgcgtaccag     1080

cagggccaga accagctcta taacgagctc aatctaggac gaagagagga gtacgatgtt     1140

ttggacaaga gacgtggccg ggaccctgag atggggggaa agccgagaag gaagaaccct     1200

caggaaggcc tgtacaatga actgcagaaa gataagatgg cggaggccta cagtgagatt     1260

gggatgaaag gcgagcgccg gaggggcaag gggcacgatg gcctttacca gggtctcagt     1320

acagccacca aggacaccta cgacgccctt cacatgcagg ccctgcctcc tcgcagagcc     1380

gagggcaggg gaagtcttct aacatgcggg gacgtggagg aaaatcccgg gcccatggcc     1440

gtgcccactc aggtcctggg gttgttgcta ctgtggctta cagatgccag atgtgacatc     1500

cagatgacac agtctccatc ttccctgtct gcatctgtcg gagatcgcgt caccatcacc     1560

tgtcgagcaa gtgaggacat ttattttaat ttagtgtggt atcagcagaa accaggaaag     1620

gcccctaagc tcctgatcta tgatacaaat cgcttggcag atggggtccc atcacggttc     1680

agtggctctg gatctggcac acagtatact ctaaccataa gtagcctgca acccgaagat     1740

ttcgcaacct attattgtca acactataag aattatccgc tcacgttcgg tcaggggacc     1800

aagctggaaa tcaaaagatc tggtggcgga gggtcaggag gcggaggcag cggaggcggt     1860

ggctcgggag gcggaggctc gagatctgag gtgcagttgg tggagtctgg gggcggcttg     1920

gtgcagcctg gagggtccct gaggctctcc tgtgcagcct caggattcac tctcagtaat     1980

tatggcatgc actggatcag gcaggctcca gggaagggtc tggagtgggt ctcgtctatt     2040

agtcttaatg gtggtagcac ttactatcga gactccgtga agggccgatt cactatctcc     2100

agggacaatg caaaaagcac cctctacctt caaatgaata gtctgagggc cgaggacacg     2160

gccgtctatt actgtgcagc acaggacgct tatacgggag gttactttga ttactggggc     2220

caaggaacgc tggtcacagt ctcgtctatg gatcccgccg agcccaaatc tcctgacaaa     2280

actcacacat gcccaccgtg cccagcacct cccgtggccg gcccgtcagt cttcctcttc     2340

cccccaaaac ccaaggacac cctcatgatc gcccggaccc ctgaggtcac atgcgtggtg     2400

gtggacgtga gccacgaaga ccctgaggtc aagttcaact ggtacgtgga cggcgtggag     2460

gtgcataatg ccaagacaaa gccgcgggag gagcagtaca acagcacgta ccgtgtggtc     2520

agcgtcctca ccgtcctgca ccaggactgg ctgaatggca aggagtacaa gtgcaaggtc     2580

tccaacaaag ccctcccagc ccccatcgag aaaaccatct ccaaagccaa agggcagccc     2640

cgagaaccac aggtgtacac cctgccccca tcccgggatg agctgaccaa gaaccaggtc     2700

agcctgacct gcctggtcaa aggcttctat cccagcgaca tcgccgtgga gtgggagagc     2760

aatgggcaac cggagaacaa ctacaagacc acgcctcccg tgctggactc cgacggctcc     2820

ttcttcctct acagcaagct caccgtggac aagagcaggt ggcagcaggg gaacgtcttc     2880

tcatgctccg tgatgcatga ggccctgcac aatcactata cccagaaatc tctgagtctg     2940

agcccaggca agaaggaccc caagttctgg gtcctggtgg tggtgggagg cgtgctggcc     3000

tgttactctc tcctggtgac cgtggccttc atcatctttt gggtgcgctc ccgggtgaag     3060

ttttctcgct ctgccgatgc cccagcctat cagcagggcc agaatcagct gtacaatgaa     3120

ctgaacctgg gcaggcggga ggagtacgac gtgctggata agcggagagg cagagacccc     3180

gagatgggcg gcaaaccacg gcgcaaaaat ccccaggagg gactctataa cgagctgcag     3240

aaggacaaaa tggccgaggc ctattccgag atcggcatga agggagagag aagacgcgga     3300

aagggccacg acggcctgta tcagggattg tccaccgcta caaaagatac atatgatgcc     3360

ctgcacatgc aggccctgcc acccagatga                                      3390


<210>  14
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site

<400>  14

Asp Ile Glu Thr Asn Pro Gly Pro 
1               5               


<210>  15
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site

<400>  15

Asp Val Glu Thr Asn Pro Gly Pro 
1               5               


<210>  16
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site

<400>  16

Asp Val Glu Met Asn Pro Gly Pro 
1               5               


<210>  17
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site

<400>  17

Asp Val Glu Ser Asn Pro Gly Pro 
1               5               


<210>  18
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site

<400>  18

Asp Met Glu Ser Asn Pro Gly Pro 
1               5               


<210>  19
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site

<400>  19

Asp Val Glu Leu Asn Pro Gly Pro 
1               5               


<210>  20
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site

<400>  20

Asp Val Glu Glu Asn Pro Gly Pro 
1               5               


<210>  21
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site

<400>  21

Asp Ile Glu Leu Asn Pro Gly Pro 
1               5               


<210>  22
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site

<400>  22

Asp Ile Glu Gln Asn Pro Gly Pro 
1               5               


<210>  23
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site

<400>  23

Asp Ser Glu Phe Asn Pro Gly Pro 
1               5               


<210>  24
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  24

Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  25
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  25

Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Gln Ser Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  26
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  26

Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ile Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  27
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  27

Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Phe Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  28
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  28

Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser His 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  29
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  29

Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Glu 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  30
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  30

Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Gln 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  31
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  31

Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn 
1               5                   10                  15      


Pro Gly Gly 
            


<210>  32
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2A-like sequence

<400>  32

Tyr His Ala Asp Tyr Tyr Lys Gln Arg Leu Ile His Asp Val Glu Met 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  33
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2A-like sequence

<400>  33

His Tyr Ala Gly Tyr Phe Ala Asp Leu Leu Ile His Asp Ile Glu Thr 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  34
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2A-like sequence

<400>  34

Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  35
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2A-like sequence

<400>  35

Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  36
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2A-like sequence

<400>  36

Ala Ala Arg Gln Met Leu Leu Leu Leu Ser Gly Asp Val Glu Thr Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  37
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2A-like sequence

<400>  37

Arg Ala Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  38
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2A-like sequence

<400>  38

Thr Arg Ala Glu Ile Glu Asp Glu Leu Ile Arg Ala Gly Ile Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  39
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2A-like sequence

<400>  39

Thr Arg Ala Glu Ile Glu Asp Glu Leu Ile Arg Ala Asp Ile Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  40
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2A-like sequence

<400>  40

Ala Lys Phe Gln Ile Asp Lys Ile Leu Ile Ser Gly Asp Val Glu Leu 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  41
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2A-like sequence

<400>  41

Ser Ser Ile Ile Arg Thr Lys Met Leu Val Ser Gly Asp Val Glu Glu 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  42
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2A-like sequence

<400>  42

Cys Asp Ala Gln Arg Gln Lys Leu Leu Leu Ser Gly Asp Ile Glu Gln 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  43
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2A-like sequence

<400>  43

Tyr Pro Ile Asp Phe Gly Gly Phe Leu Val Lys Ala Asp Ser Glu Phe 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  44
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  44

Val Thr Glu Leu Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro 
1               5                   10                  15      


Arg Pro Leu Ala Ile His Pro Thr Glu Ala Arg His Lys Gln Lys Ile 
            20                  25                  30          


Val Ala Pro Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala 
        35                  40                  45              


Gly Asp Val Glu Ser Asn Pro Gly Pro 
    50                  55          


<210>  45
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  45

Leu Leu Ala Ile His Pro Thr Glu Ala Arg His Lys Gln Lys Ile Val 
1               5                   10                  15      


Ala Pro Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly 
            20                  25                  30          


Asp Val Glu Ser Asn Pro Gly Pro 
        35                  40  


<210>  46
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  46

Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro Val Lys Gln Thr Leu 
1               5                   10                  15      


Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly 
            20                  25                  30          


Pro 
    


<210>  47
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage sequence

<400>  47

Ala Pro Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly 
1               5                   10                  15      


Asp Val Glu Ser Asn Pro Gly Pro 
            20                  


<210>  48
<211>  136
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  marker/suicide polypeptide

<400>  48

Cys Pro Tyr Ser Asn Pro Ser Leu Cys Ser Gly Gly Gly Gly Ser Glu 
1               5                   10                  15      


Leu Pro Thr Gln Gly Thr Phe Ser Asn Val Ser Thr Asn Val Ser Pro 
            20                  25                  30          


Ala Lys Pro Thr Thr Thr Ala Cys Pro Tyr Ser Asn Pro Ser Leu Cys 
        35                  40                  45              


Ser Gly Gly Gly Gly Ser Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro 
    50                  55                  60                  


Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro 
65                  70                  75                  80  


Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
                85                  90                  95      


Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
            100                 105                 110         


Ser Leu Val Ile Thr Leu Tyr Cys Asn His Arg Asn Arg Arg Arg Val 
        115                 120                 125             


Cys Lys Cys Pro Arg Pro Val Val 
    130                 135     


<210>  49
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  intracellular retention signal


<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  49

Asn Pro Phe Xaa 
1               


<210>  50
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  intracellular retention signal

<400>  50

Lys Asp Glu Leu 
1               


<210>  51
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  intracellular retention signal


<220>
<221>  misc_feature
<222>  (3)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  51

Lys Lys Xaa Xaa 
1               


<210>  52
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  intracellular retention signal


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  Xaa can be any naturally occurring amino acid

<400>  52

Lys Xaa Lys Xaa Xaa 
1               5   


<210>  53
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  intracellular retention signal


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  53

Asn Pro Xaa Tyr 
1               


<210>  54
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  intracellular retention signal


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is an amino acid with a bulky hydrophobic side chain

<400>  54

Tyr Xaa Xaa Xaa 
1               


<210>  55
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  intracellular retention signal


<220>
<221>  misc_feature
<222>  (3)..(5)
<223>  Xaa can be any naturally occurring amino acid

<400>  55

Asp Glu Xaa Xaa Xaa Leu Leu Ile 
1               5               


<210>  56
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  intracellular retention signal


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  56

Asp Xaa Xaa Leu Leu 
1               5   


<210>  57
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  intracellular retention signal

<400>  57

Asp Pro Phe Trp 
1               


<210>  58
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  intracellular retention signal


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  58

Phe Xaa Asp Xaa Phe 
1               5   


<210>  59
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  intracellular retention signal


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa is an amino acid with a bulky hydrophobic side chain

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is an amino acid with a bulky hydrophobic side chain

<400>  59

Leu Xaa Xaa Xaa Asp Glu 
1               5       


<210>  60
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  intracellular retention signal

<400>  60

Leu Leu Asp Leu Leu 
1               5   


<210>  61
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  intracellular retention signal

<400>  61

Pro Trp Asp Leu Trp 
1               5   


<210>  62
<211>  20
<212>  PRT
<213>  Mus musculus

<400>  62

Met Glu Thr Asp Thr Leu Ile Leu Trp Val Leu Leu Leu Leu Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly 
            20  


<210>  63
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signal peptide derived from Human CD8a

<400>  63

Met Ser Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


<210>  64
<211>  248
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFv aCD19

<400>  64

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Lys Ala Gly Gly Gly 
            100                 105                 110         


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser 
    130                 135                 140                 


Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp 
145                 150                 155                 160 


Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp 
                165                 170                 175     


Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu 
            180                 185                 190         


Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe 
        195                 200                 205             


Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys 
    210                 215                 220                 


Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly 
225                 230                 235                 240 


Gln Gly Thr Ser Val Thr Val Ser 
                245             


<210>  65
<211>  47
<212>  PRT
<213>  Homo sapiens

<400>  65

Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile 
1               5                   10                  15      


Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala 
            20                  25                  30          


Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile 
        35                  40                  45          


<210>  66
<211>  27
<212>  PRT
<213>  Homo sapiens

<400>  66

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25          


<210>  67
<211>  113
<212>  PRT
<213>  Homo sapiens

<400>  67

Arg Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln 
1               5                   10                  15      


Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu 
            20                  25                  30          


Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly 
        35                  40                  45              


Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
    50                  55                  60                  


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
65                  70                  75                  80  


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
                85                  90                  95      


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
            100                 105                 110         


Arg 
    


<210>  68
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2A peptide

<400>  68

Arg Ala Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  69
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signal peptide derived from mouse Ig kappa

<400>  69

Met Ala Val Pro Thr Gln Val Leu Gly Leu Leu Leu Leu Trp Leu Thr 
1               5                   10                  15      


Asp Ala 
        


<210>  70
<211>  254
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFv aCD33

<400>  70

Arg Cys Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser 
1               5                   10                  15      


Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Glu Asp Ile Tyr 
            20                  25                  30          


Phe Asn Leu Val Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu 
        35                  40                  45              


Leu Ile Tyr Asp Thr Asn Arg Leu Ala Asp Gly Val Pro Ser Arg Phe 
    50                  55                  60                  


Ser Gly Ser Gly Ser Gly Thr Gln Tyr Thr Leu Thr Ile Ser Ser Leu 
65                  70                  75                  80  


Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln His Tyr Lys Asn Tyr 
                85                  90                  95      


Pro Leu Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Ser Gly 
            100                 105                 110         


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Arg Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu 
    130                 135                 140                 


Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe 
145                 150                 155                 160 


Thr Leu Ser Asn Tyr Gly Met His Trp Ile Arg Gln Ala Pro Gly Lys 
                165                 170                 175     


Gly Leu Glu Trp Val Ser Ser Ile Ser Leu Asn Gly Gly Ser Thr Tyr 
            180                 185                 190         


Tyr Arg Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala 
        195                 200                 205             


Lys Ser Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr 
    210                 215                 220                 


Ala Val Tyr Tyr Cys Ala Ala Gln Asp Ala Tyr Thr Gly Gly Tyr Phe 
225                 230                 235                 240 


Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Met 
                245                 250                 


<210>  71
<211>  231
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hinge and Fc derived from human IgG1

<400>  71

Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
1               5                   10                  15      


Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
            20                  25                  30          


Asp Thr Leu Met Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val Val 
        35                  40                  45              


Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 
    50                  55                  60                  


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 
65                  70                  75                  80  


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
                85                  90                  95      


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 
            100                 105                 110         


Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
        115                 120                 125             


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys 
    130                 135                 140                 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
145                 150                 155                 160 


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
                165                 170                 175     


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
            180                 185                 190         


Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 
        195                 200                 205             


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
    210                 215                 220                 


Leu Ser Leu Ser Pro Gly Lys 
225                 230     


<210>  72
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  72

Lys Asp Pro Lys 
1               


<210>  73
<211>  27
<212>  PRT
<213>  Homo sapiens

<400>  73

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25          


<210>  74
<211>  112
<212>  PRT
<213>  Homo sapiens

<400>  74

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  75
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  nucleic acid construct comprising a tyrp-1 retention protein 
       sequence

<400>  75

Arg Ala Arg Arg Ser Met Asp Glu Ala Asn Gln Pro Leu Leu Thr Asp 
1               5                   10                  15      


Gln Tyr Gln Cys Tyr Ala Glu Glu Tyr Glu Lys Leu Gln Asn Pro Asn 
            20                  25                  30          


Gln Ser Val Val 
        35      


<210>  76
<211>  4
<212>  PRT
<213>  Mus musculus

<400>  76

Met Glu Thr Asp 
1               


<210>  77
<211>  11
<212>  PRT
<213>  Mus musculus

<400>  77

Thr Leu Ile Leu Trp Val Leu Leu Leu Leu Val 
1               5                   10      


<210>  78
<211>  5
<212>  PRT
<213>  Mus musculus

<400>  78

Pro Gly Ser Thr Gly 
1               5   


<210>  79
<211>  25
<212>  PRT
<213>  Homo sapiens

<400>  79

Met Asn Arg Gly Val Pro Phe Arg His Leu Leu Leu Val Leu Gln Leu 
1               5                   10                  15      


Ala Leu Leu Pro Ala Ala Thr Gln Gly 
            20                  25  


<210>  80
<211>  23
<212>  PRT
<213>  Homo sapiens

<400>  80

Met Tyr Leu Trp Leu Lys Leu Leu Ala Phe Gly Phe Ala Phe Leu Asp 
1               5                   10                  15      


Thr Glu Val Phe Val Thr Gly 
            20              


<210>  81
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  81

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


<210>  82
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  82

Met Arg Pro Arg Leu Trp Leu Leu Leu Ala Ala Gln Leu Thr Val Leu 
1               5                   10                  15      


His Gly Asn Ser Val 
            20      


<210>  83
<211>  24
<212>  PRT
<213>  Homo sapiens

<400>  83

Met Ser Phe Pro Cys Lys Phe Val Ala Ser Phe Leu Leu Ile Phe Asn 
1               5                   10                  15      


Val Ser Ser Lys Gly Ala Val Ser 
            20                  


<210>  84
<211>  24
<212>  PRT
<213>  Homo sapiens

<400>  84

Met Pro Met Gly Ser Leu Gln Pro Leu Ala Thr Leu Tyr Leu Leu Gly 
1               5                   10                  15      


Met Leu Val Ala Ser Cys Leu Gly 
            20                  


<210>  85
<211>  25
<212>  PRT
<213>  Homo sapiens

<400>  85

Met Ala Gly Pro Pro Arg Leu Leu Leu Leu Pro Leu Leu Leu Ala Leu 
1               5                   10                  15      


Ala Arg Gly Leu Pro Gly Ala Leu Ala 
            20                  25  


<210>  86
<211>  24
<212>  PRT
<213>  Homo sapiens

<400>  86

Met Ser Ala Pro Lys Leu Leu Ser Leu Gly Cys Ile Phe Phe Pro Leu 
1               5                   10                  15      


Leu Leu Phe Gln Gln Ala Arg Ala 
            20                  


<210>  87
<211>  19
<212>  PRT
<213>  Homo sapiens

<400>  87

Met Arg Phe Phe Val Pro Leu Phe Leu Val Gly Ile Leu Phe Pro Ala 
1               5                   10                  15      


Ile Leu Ala 
            


<210>  88
<211>  23
<212>  PRT
<213>  Homo sapiens

<400>  88

Met Pro Gly Phe Leu Val Arg Ile Leu Pro Leu Leu Leu Val Leu Leu 
1               5                   10                  15      


Leu Leu Gly Pro Thr Arg Gly 
            20              


<210>  89
<211>  24
<212>  PRT
<213>  Homo sapiens

<400>  89

Met Lys Arg Phe Leu Phe Leu Leu Leu Thr Ile Ser Leu Leu Val Met 
1               5                   10                  15      


Val Gln Ile Gln Thr Gly Leu Ser 
            20                  


<210>  90
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  90

Met Gln Gly Pro Pro Leu Leu Thr Ala Ala His Leu Leu Cys Val Cys 
1               5                   10                  15      


Thr Ala Ala Leu Ala 
            20      


<210>  91
<211>  22
<212>  PRT
<213>  Homo sapiens

<400>  91

Met Gly Pro Thr Ser Gly Pro Ser Leu Leu Leu Leu Leu Leu Thr His 
1               5                   10                  15      


Leu Pro Leu Ala Leu Gly 
            20          


<210>  92
<211>  18
<212>  PRT
<213>  Homo sapiens

<400>  92

Met Gln Pro Ile Leu Leu Leu Leu Ala Phe Leu Leu Leu Pro Arg Ala 
1               5                   10                  15      


Asp Ala 
        


<210>  93
<211>  20
<212>  PRT
<213>  Homo sapiens

<400>  93

Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro 
1               5                   10                  15      


Asp Thr Thr Arg 
            20  


<210>  94
<211>  20
<212>  PRT
<213>  Homo sapiens

<400>  94

Met Val Leu Gln Thr Gln Val Phe Ile Ser Leu Leu Leu Trp Ile Ser 
1               5                   10                  15      


Gly Ala Tyr Gly 
            20  


<210>  95
<211>  19
<212>  PRT
<213>  Homo sapiens

<400>  95

Met Ala Trp Ala Pro Leu Leu Leu Thr Leu Leu Ala His Cys Thr Asp 
1               5                   10                  15      


Cys Trp Ala 
            


<210>  96
<211>  22
<212>  PRT
<213>  Homo sapiens

<400>  96

Met Leu Lys Pro Ser Leu Pro Phe Thr Ser Leu Leu Phe Leu Gln Leu 
1               5                   10                  15      


Pro Leu Leu Gly Val Gly 
            20          


<210>  97
<211>  20
<212>  PRT
<213>  Homo sapiens

<400>  97

Met Ala Trp Ala Ser Arg Leu Gly Leu Leu Leu Ala Leu Leu Leu Pro 
1               5                   10                  15      


Val Val Gly Ala 
            20  


<210>  98
<211>  23
<212>  PRT
<213>  Homo sapiens

<400>  98

Met Gly Val Pro Arg Pro Gln Pro Trp Ala Leu Gly Leu Leu Leu Phe 
1               5                   10                  15      


Leu Leu Pro Gly Ser Leu Gly 
            20              


<210>  99
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ITAM motif


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa may be Leu or Ile

<400>  99

Tyr Xaa Xaa Xaa 
1               


<210>  100
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  basic amino acid furin target sequence


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa may be Arg or Lys

<400>  100

Arg Xaa Xaa Arg 
1               


<210>  101
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  consensus Tobacco Etch Virus (TEV) cleavage site

<400>  101

Glu Asn Leu Tyr Phe Gln Ser 
1               5           


<210>  102
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  motif involved in cleavage activity


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  102

Asp Xaa Glu Xaa Asn Pro Gly Pro 
1               5               


<210>  103
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa may be Ile, Val, Met or Ser

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa may be Thr, Met, Ser, Leu, Glu, Gln or Phe

<400>  103

Asp Xaa Glu Xaa Asn Pro Gly Pro 
1               5               


<210>  104
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C-terminal signal, motif of postassium channels


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<400>  104

Arg Xaa Arg Arg 
1               


<210>  105
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  105

Ile Asn Phe Asp Asn Pro Val Tyr Gln Lys Thr Thr 
1               5                   10          


<210>  106
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  106

Val Glu Ile Gly Asn Pro Thr Tyr Lys Met Tyr Glu 
1               5                   10          


<210>  107
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  107

Thr Asn Phe Thr Asn Pro Val Tyr Ala Thr Leu Tyr 
1               5                   10          


<210>  108
<211>  12
<212>  PRT
<213>  Drosophila sp.

<400>  108

Gly Asn Phe Ala Asn Pro Val Tyr Glu Ser Met Tyr 
1               5                   10          


<210>  109
<211>  12
<212>  PRT
<213>  Caenorhabditis elegans

<400>  109

Thr Thr Phe Thr Asn Pro Val Tyr Glu Leu Glu Asp 
1               5                   10          


<210>  110
<211>  12
<212>  PRT
<213>  Caenorhabditis elegans

<400>  110

Leu Arg Val Asp Asn Pro Leu Tyr Asp Pro Asp Ser 
1               5                   10          


<210>  111
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  111

Ile Ile Phe Glu Asn Pro Met Tyr Ser Ala Arg Asp 
1               5                   10          


<210>  112
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  112

Thr Asn Phe Glu Asn Pro Ile Tyr Ala Gln Met Glu 
1               5                   10          


<210>  113
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  113

Asp Thr Gly Glu Asn Pro Ile Tyr Lys Ser Ala Val 
1               5                   10          


<210>  114
<211>  11
<212>  PRT
<213>  Homo sapiens

<400>  114

Thr Thr Val Val Asn Pro Lys Tyr Glu Gly Lys 
1               5                   10      


<210>  115
<211>  12
<212>  PRT
<213>  Drosophila sp.

<400>  115

Trp Asp Thr Glu Asn Pro Ile Tyr Lys Gln Ala Thr 
1               5                   10          


<210>  116
<211>  11
<212>  PRT
<213>  Drosophila sp.

<400>  116

Ser Thr Phe Lys Asn Pro Met Tyr Ala Gly Lys 
1               5                   10      


<210>  117
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  117

His Gly Tyr Glu Asn Pro Thr Tyr Arg Phe Leu Glu 
1               5                   10          


<210>  118
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  118

Asn Gly Tyr Glu Asn Pro Thr Tyr Lys Phe Phe Glu 
1               5                   10          


<210>  119
<211>  12
<212>  PRT
<213>  Drosophila sp.

<400>  119

Asn Gly Tyr Glu Asn Pro Thr Tyr Lys Tyr Phe Glu 
1               5                   10          


<210>  120
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  120

Tyr Ala Ser Ser Asn Pro Glu Tyr Leu Ser Ala Ser 
1               5                   10          


<210>  121
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  121

Gly Ser Val Gln Asn Pro Val Tyr His Asn Gln Pro 
1               5                   10          


<210>  122
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  122

Thr Ala Val Gly Asn Pro Glu Tyr Leu Asn Thr Val 
1               5                   10          


<210>  123
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  123

Ile Ser Leu Asp Asn Pro Asp Tyr Gln Gln Asp Phe 
1               5                   10          


<210>  124
<211>  11
<212>  PRT
<213>  Homo sapiens

<400>  124

Arg Lys Arg Ser His Ala Gly Tyr Gln Thr Ile 
1               5                   10      


<210>  125
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  125

Lys His His His Ala Gly Tyr Glu Gln Phe 
1               5                   10  


<210>  126
<211>  11
<212>  PRT
<213>  Gallus gallus

<400>  126

Lys Lys His His Asn Thr Gly Tyr Glu Gln Phe 
1               5                   10      


<210>  127
<211>  11
<212>  PRT
<213>  Gallus gallus

<400>  127

Arg Arg Lys Ser Arg Thr Gly Tyr Gln Ser Val 
1               5                   10      


<210>  128
<211>  11
<212>  PRT
<213>  Gallus gallus

<400>  128

Arg Arg Lys Ser Tyr Ala Gly Tyr Gln Thr Leu 
1               5                   10      


<210>  129
<211>  12
<212>  PRT
<213>  Drosophila sp.

<400>  129

Arg Arg Arg Ser Thr Ser Arg Gly Tyr Met Ser Phe 
1               5                   10          


<210>  130
<211>  11
<212>  PRT
<213>  Lumbricus terrestris

<400>  130

Arg Lys Arg Ser Arg Arg Gly Tyr Glu Ser Val 
1               5                   10      


<210>  131
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  131

Lys Ser Ile Arg Ser Gly Tyr Glu Val Met 
1               5                   10  


<210>  132
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  132

His Cys Gly Gly Pro Arg Pro Gly Tyr Glu Thr Leu 
1               5                   10          


<210>  133
<211>  12
<212>  PRT
<213>  Mus musculus

<400>  133

His Cys Arg Thr Arg Arg Ala Glu Tyr Glu Thr Leu 
1               5                   10          


<210>  134
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  134

Arg Arg Arg Pro Ser Ala Tyr Gln Ala Leu 
1               5                   10  


<210>  135
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  135

Arg Arg Arg Ser Tyr Gln Asn Ile Pro 
1               5                   


<210>  136
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  136

Lys Lys His Cys Ser Tyr Gln Asp Ile Leu 
1               5                   10  


<210>  137
<211>  10
<212>  PRT
<213>  Mus musculus

<400>  137

Arg Arg Arg Ser Ala Tyr Gln Asp Ile Arg 
1               5                   10  


<210>  138
<211>  11
<212>  PRT
<213>  Rattus norvegicus

<400>  138

Arg Lys Arg Arg Arg Ser Tyr Gln Asp Ile Met 
1               5                   10      


<210>  139
<211>  13
<212>  PRT
<213>  Rattus norvegicus

<400>  139

Lys Phe Cys Lys Ser Lys Glu Arg Asn Tyr His Thr Leu 
1               5                   10              


<210>  140
<211>  14
<212>  PRT
<213>  Drosophila sp.

<400>  140

Lys Phe Tyr Lys Ala Arg Asn Glu Arg Asn Tyr His Thr Leu 
1               5                   10                  


<210>  141
<211>  14
<212>  PRT
<213>  Homo sapiens

<400>  141

Lys Ile Arg Leu Arg Cys Gln Ser Ser Gly Tyr Gln Arg Ile 
1               5                   10                  


<210>  142
<211>  14
<212>  PRT
<213>  Mus musculus

<400>  142

Lys Ile Arg Gln Arg His Gln Ser Ser Ala Tyr Gln Arg Ile 
1               5                   10                  


<210>  143
<211>  15
<212>  PRT
<213>  Homo sapiens

<400>  143

His Phe Cys Leu Tyr Arg Lys Arg Pro Gly Tyr Asp Gln Leu Asn 
1               5                   10                  15  


<210>  144
<211>  11
<212>  PRT
<213>  Homo sapiens

<400>  144

Ser Leu Ser Arg Gly Ser Gly Tyr Lys Glu Ile 
1               5                   10      


<210>  145
<211>  13
<212>  PRT
<213>  Homo sapiens

<400>  145

Arg Arg Leu Arg Lys Gly Tyr Thr Pro Leu Met Glu Thr 
1               5                   10              


<210>  146
<211>  14
<212>  PRT
<213>  Homo sapiens

<400>  146

Arg Arg Ala Gly His Ser Ser Tyr Thr Pro Leu Pro Gly Ser 
1               5                   10                  


<210>  147
<211>  18
<212>  PRT
<213>  Dictyostelium sp.

<400>  147

Lys Lys Leu Arg Gln Gln Lys Gln Gln Gly Tyr Gln Ala Ile Ile Asn 
1               5                   10                  15      


Asn Glu 
        


<210>  148
<211>  15
<212>  PRT
<213>  Dictyostelium sp.

<400>  148

Arg Ser Lys Ser Asn Gln Asn Gln Ser Tyr Asn Leu Ile Gln Leu 
1               5                   10                  15  


<210>  149
<211>  17
<212>  PRT
<213>  Dictyostelium sp.

<400>  149

Arg Lys Thr Phe Tyr Asn Asn Asn Gln Tyr Asn Gly Tyr Asn Ile Ile 
1               5                   10                  15      


Asn 
    


<210>  150
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  150

Pro Leu Ser Tyr Thr Arg Phe Ser Leu Ala 
1               5                   10  


<210>  151
<211>  11
<212>  PRT
<213>  Homo sapiens

<400>  151

Met Thr Lys Glu Tyr Gln Asp Leu Gln His Leu 
1               5                   10      


<210>  152
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  152

Ser Tyr Lys Tyr Ser Lys Val Asn Lys Glu 
1               5                   10  


<210>  153
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  153

Pro Ala Ala Tyr Arg Gly Val Gly Asp Asp 
1               5                   10  


<210>  154
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  154

Thr Gly Val Tyr Val Lys Met Pro Pro Thr 
1               5                   10  


<210>  155
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  155

Leu Ile Ser Tyr Lys Gly Leu Pro Pro Glu 
1               5                   10  


<210>  156
<211>  11
<212>  PRT
<213>  Rattus norvegicus

<400>  156

Ala Ser Asp Tyr Gln Arg Leu Asn Leu Lys Leu 
1               5                   10      


<210>  157
<211>  11
<212>  PRT
<213>  Human immunodeficiency virus type 1

<400>  157

Arg Gln Gly Tyr Ser Pro Leu Ser Phe Gln Thr 
1               5                   10      


<210>  158
<211>  19
<212>  PRT
<213>  Homo sapiens

<400>  158

Arg Met Gln Ala Gln Pro Pro Gly Tyr Arg His Val Ala Asp Gly Glu 
1               5                   10                  15      


Asp His Ala 
            


<210>  159
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Dileucine-based sorting signal


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa may be Asp or Glu

<220>
<221>  misc_feature
<222>  (2)..(4)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa may be Leu or Ile

<400>  159

Xaa Xaa Xaa Xaa Leu Leu Xaa 
1               5           


<210>  160
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  160

Ser Asp Lys Gln Thr Leu Leu Pro Asn 
1               5                   


<210>  161
<211>  9
<212>  PRT
<213>  Rattus norvegicus

<400>  161

Asp Glu Arg Ala Pro Leu Ile Arg Thr 
1               5                   


<210>  162
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  162

Gln Glu Lys Asp Pro Leu Leu Lys Asn 
1               5                   


<210>  163
<211>  9
<212>  PRT
<213>  Coturnix sp.

<400>  163

Thr Glu Arg Asn Pro Leu Leu Lys Ser 
1               5                   


<210>  164
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  164

Gly Glu Asn Ser Pro Leu Leu Ser Gly 
1               5                   


<210>  165
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  165

Glu Glu Lys Gln Pro Leu Leu Met Glu 
1               5                   


<210>  166
<211>  9
<212>  PRT
<213>  Oryzias latipes

<400>  166

Gly Glu Arg Gln Pro Leu Leu Gln Ser 
1               5                   


<210>  167
<211>  9
<212>  PRT
<213>  Gallus gallus

<400>  167

Pro Glu Ile Gln Pro Leu Leu Thr Glu 
1               5                   


<210>  168
<211>  9
<212>  PRT
<213>  Carassius auratus

<400>  168

Glu Gly Arg Gln Pro Leu Leu Gly Asp 
1               5                   


<210>  169
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  169

Glu Ala Asn Gln Pro Leu Leu Thr Asp 
1               5                   


<210>  170
<211>  9
<212>  PRT
<213>  Gallus gallus

<400>  170

Glu Leu His Gln Pro Leu Leu Thr Asp 
1               5                   


<210>  171
<211>  9
<212>  PRT
<213>  Danio rerio

<400>  171

Arg Glu Phe Glu Pro Leu Leu Asn Ala 
1               5                   


<210>  172
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  172

Glu Glu Lys Met Ala Ile Leu Met Asp 
1               5                   


<210>  173
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  173

Glu Glu Lys Leu Ala Ile Leu Ser Gln 
1               5                   


<210>  174
<211>  9
<212>  PRT
<213>  Mus musculus

<400>  174

Ser Glu Arg Asp Val Leu Leu Asp Glu 
1               5                   


<210>  175
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  175

Ser Glu Arg Arg Asn Leu Leu Glu Asp 
1               5                   


<210>  176
<211>  9
<212>  PRT
<213>  Rattus norvegicus

<400>  176

Asp Asp Ser Gly Asp Leu Leu Pro Gly 
1               5                   


<210>  177
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  177

Ser Gln Ile Lys Arg Leu Leu Ser Glu 
1               5                   


<210>  178
<211>  9
<212>  PRT
<213>  Felis catus

<400>  178

Ser His Ile Lys Arg Leu Leu Ser Glu 
1               5                   


<210>  179
<211>  9
<212>  PRT
<213>  Mus musculus

<400>  179

Arg Arg Thr Pro Ser Leu Leu Glu Gln 
1               5                   


<210>  180
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  180

His Arg Thr Pro Ser Leu Leu Glu Gln 
1               5                   


<210>  181
<211>  10
<212>  PRT
<213>  Rattus norvegicus

<400>  181

Glu Pro Arg Gly Ser Arg Leu Leu Val Arg 
1               5                   10  


<210>  182
<211>  19
<212>  PRT
<213>  Homo sapiens

<400>  182

Met Asp Asp Gln Arg Asp Leu Ile Ser Asn Asn Glu Gln Leu Pro Met 
1               5                   10                  15      


Leu Gly Arg 
            


<210>  183
<211>  19
<212>  PRT
<213>  Mus musculus

<400>  183

Met Asp Asp Gln Arg Asp Leu Ile Ser Asn His Glu Gln Leu Pro Ile 
1               5                   10                  15      


Leu Gly Asn 
            


<210>  184
<211>  20
<212>  PRT
<213>  Gallus gallus

<400>  184

Met Ala Glu Glu Gln Arg Asp Leu Ile Ser Ser Asp Gly Ser Ser Gly 
1               5                   10                  15      


Val Leu Pro Ile 
            20  


<210>  185
<211>  23
<212>  PRT
<213>  Danio rerio

<400>  185

Met Glu Pro Asp His Gln Asn Glu Ser Leu Ile Gln Arg Val Pro Ser 
1               5                   10                  15      


Ala Glu Thr Ile Leu Gly Arg 
            20              


<210>  186
<211>  23
<212>  PRT
<213>  Danio rerio

<400>  186

Met Ser Ser Glu Gly Asn Glu Thr Pro Leu Ile Ser Asp Gln Ser Ser 
1               5                   10                  15      


Val Asn Met Gly Pro Gln Pro 
            20              


<210>  187
<211>  24
<212>  PRT
<213>  Trypanosoma sp.

<400>  187

Arg Pro Arg Arg Arg Thr Glu Glu Asp Glu Leu Leu Pro Glu Glu Ala 
1               5                   10                  15      


Glu Gly Leu Ile Asp Pro Gln Asn 
            20                  


<210>  188
<211>  19
<212>  PRT
<213>  Homo sapiens

<400>  188

Pro Asp Lys His Ser Leu Leu Val Gly Asp Phe Arg Glu Asp Asp Asp 
1               5                   10                  15      


Thr Ala Leu 
            


<210>  189
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  189

Thr Glu Arg Glu Arg Leu Leu Asn Phe 
1               5                   


<210>  190
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  190

Val Glu Thr Asp Asp Leu Ile Leu 
1               5               


<210>  191
<211>  7
<212>  PRT
<213>  Caenorhabditis elegans

<400>  191

Phe Glu Asn Asp Ser Leu Leu 
1               5           


<210>  192
<211>  9
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  192

Asn Glu Gln Ser Pro Leu Leu His Asn 
1               5                   


<210>  193
<211>  8
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  193

Ser Glu Gln Thr Arg Leu Val Pro 
1               5               


<210>  194
<211>  8
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  194

Glu Val Asp Leu Asp Leu Leu Lys 
1               5               


<210>  195
<211>  13
<212>  PRT
<213>  Homo sapiens

<400>  195

Ser Phe His Asp Asp Ser Asp Glu Asp Leu Leu His Ile 
1               5                   10              


<210>  196
<211>  13
<212>  PRT
<213>  Bos taurus

<400>  196

Thr Phe His Asp Asp Ser Asp Glu Asp Leu Leu His Val 
1               5                   10              


<210>  197
<211>  13
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  197

Ser Phe His Asp Asp Ser Asp Glu Asp Leu Leu Asn Ile 
1               5                   10              


<210>  198
<211>  13
<212>  PRT
<213>  Gallus gallus

<400>  198

Ser Phe His Asp Asp Ser Asp Glu Asp Leu Leu Asn Val 
1               5                   10              


<210>  199
<211>  13
<212>  PRT
<213>  Homo sapiens

<400>  199

Glu Glu Ser Glu Glu Arg Asp Asp His Leu Leu Pro Met 
1               5                   10              


<210>  200
<211>  13
<212>  PRT
<213>  Gallus gallus

<400>  200

Asp Glu Ser Glu Glu Arg Asp Asp His Leu Leu Pro Met 
1               5                   10              


<210>  201
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  201

Gly Tyr His Asp Asp Ser Asp Glu Asp Leu Leu Glu 
1               5                   10          


<210>  202
<211>  13
<212>  PRT
<213>  Homo sapiens

<400>  202

Ile Thr Gly Phe Ser Asp Asp Val Pro Met Val Ile Ala 
1               5                   10              


<210>  203
<211>  13
<212>  PRT
<213>  Hydra sp.

<400>  203

Ile Asn Arg Phe Ser Asp Asp Glu Pro Leu Val Val Ala 
1               5                   10              


<210>  204
<211>  13
<212>  PRT
<213>  Homo sapiens

<400>  204

Met Leu Glu Ala Ser Asp Asp Glu Ala Leu Leu Val Cys 
1               5                   10              


<210>  205
<211>  13
<212>  PRT
<213>  Homo sapiens

<400>  205

Lys Asn Glu Thr Ser Asp Asp Glu Ala Leu Leu Leu Cys 
1               5                   10              


<210>  206
<211>  12
<212>  PRT
<213>  Mus musculus

<400>  206

Trp Val Val Glu Ala Glu Asp Glu Pro Leu Leu Ala 
1               5                   10          


<210>  207
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  207

Trp Val Ala Glu Ala Glu Asp Glu Pro Leu Leu Thr 
1               5                   10          


<210>  208
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  208

His Asp Asp Phe Ala Asp Asp Ile Ser Leu Leu Lys 
1               5                   10          


<210>  209
<211>  13
<212>  PRT
<213>  Mus musculus

<400>  209

Gly Arg Asp Ser Pro Glu Asp His Ser Leu Leu Val Asn 
1               5                   10              


<210>  210
<211>  12
<212>  PRT
<213>  Peromyscus maniculatus

<400>  210

Val Arg Cys His Pro Glu Asp Asp Arg Leu Leu Gly 
1               5                   10          


<210>  211
<211>  13
<212>  PRT
<213>  Homo sapiens

<400>  211

His Arg Val Ser Gln Asp Asp Leu Asp Leu Leu Thr Ser 
1               5                   10              


<210>  212
<211>  13
<212>  PRT
<213>  Homo sapiens

<400>  212

Ala Ser Val Ser Leu Leu Asp Asp Glu Leu Met Ser Leu 
1               5                   10              


<210>  213
<211>  13
<212>  PRT
<213>  Homo sapiens

<400>  213

Ala Ser Ser Gly Leu Asp Asp Leu Asp Leu Leu Gly Lys 
1               5                   10              


<210>  214
<211>  13
<212>  PRT
<213>  Homo sapiens

<400>  214

Val Gln Asn Pro Ser Ala Asp Arg Asn Leu Leu Asp Leu 
1               5                   10              


<210>  215
<211>  13
<212>  PRT
<213>  Homo sapiens

<400>  215

Asn Ala Leu Ser Trp Leu Asp Glu Glu Leu Leu Cys Leu 
1               5                   10              


<210>  216
<211>  13
<212>  PRT
<213>  Drosophila sp.

<400>  216

Thr Val Asp Ser Ile Asp Asp Val Pro Leu Leu Ser Asp 
1               5                   10              


<210>  217
<211>  13
<212>  PRT
<213>  Mus musculus

<400>  217

Gln Glu Glu Cys Pro Ser Asp Ser Glu Glu Asp Glu Gly 
1               5                   10              


<210>  218
<211>  13
<212>  PRT
<213>  Mus musculus

<400>  218

Arg Asp Arg Asp Tyr Asp Glu Asp Asp Glu Asp Asp Ile 
1               5                   10              


<210>  219
<211>  15
<212>  PRT
<213>  Mus musculus

<400>  219

Leu Asp Glu Thr Glu Asp Asp Glu Leu Glu Tyr Asp Asp Glu Ser 
1               5                   10                  15  


<210>  220
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  220

Lys Asp Pro Asp Glu Val Glu Thr Glu Ser 
1               5                   10  


<210>  221
<211>  13
<212>  PRT
<213>  Homo sapiens

<400>  221

His Glu Phe Gln Asp Glu Thr Asp Thr Glu Glu Glu Thr 
1               5                   10              


<210>  222
<211>  14
<212>  PRT
<213>  Homo sapiens

<400>  222

Gln Glu Lys Glu Asp Asp Gly Ser Glu Ser Glu Glu Glu Tyr 
1               5                   10                  


<210>  223
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  223

Gly Glu Asp Glu Glu Ser Glu Ser Asp 
1               5                   


<210>  224
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  224

Gly Glu Asp Ser Asp Glu Glu Pro Asp His Glu Glu 
1               5                   10          


<210>  225
<211>  11
<212>  PRT
<213>  Homo sapiens

<400>  225

Leu Glu Asp Asp Ser Asp Glu Glu Glu Asp Phe 
1               5                   10      


<210>  226
<211>  9
<212>  PRT
<213>  Human cytomegalovirus

<400>  226

Lys Asp Ser Asp Glu Glu Glu Asn Val 
1               5                   


<210>  227
<211>  13
<212>  PRT
<213>  Herpesvirus 3

<400>  227

Phe Glu Asp Ser Glu Ser Thr Asp Thr Glu Glu Glu Phe 
1               5                   10              


<210>  228
<211>  9
<212>  PRT
<213>  Human immunodeficiency virus type 1

<400>  228

Leu Glu Ala Gln Glu Glu Glu Glu Val 
1               5                   


<210>  229
<211>  22
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  229

Ala Asp Asp Leu Glu Ser Gly Leu Gly Ala Glu Asp Asp Leu Glu Gln 
1               5                   10                  15      


Asp Glu Gln Leu Glu Gly 
            20          


<210>  230
<211>  12
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  230

Thr Glu Ile Asp Glu Ser Phe Glu Met Thr Asp Phe 
1               5                   10          


<210>  231
<211>  18
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  231

Thr Glu Pro Glu Glu Val Glu Asp Phe Asp Phe Asp Leu Ser Asp Glu 
1               5                   10                  15      


Asp His 
        


<210>  232
<211>  15
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  232

Phe Glu Ile Glu Glu Asp Asp Val Pro Thr Leu Glu Glu Glu His 
1               5                   10                  15  


<210>  233
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  intracellular retention signal

<400>  233

His Asp Glu Leu 
1               

