                         SEQUENCE LISTING

<110>  CureVac AG
 
<120>  Immobilized poly(N)polymerase

<130>  C9810WO2 / RN

<150>  PCT/EP2015/059611
<151>  30.04.2015

<150>  GB1507504.7
<151>  30.04.2015

<160>  203   

<170>  PatentIn version 3.5

<210>  1
<211>  454
<212>  PRT
<213>  Escherichia coli

<400>  1

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Cys Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Cys 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Cys Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala 
    450                 


<210>  2
<211>  438
<212>  PRT
<213>  Thermus aquaticus

<400>  2

Met Leu Arg Leu Ile Ala His Arg Asp Phe Pro Phe Tyr Thr Pro Lys 
1               5                   10                  15      


Gly Ala Ile Pro Val Gly Gly Ala Leu Arg Asp Leu Leu Leu Gly Glu 
            20                  25                  30          


Arg Pro Leu Asp Leu Asp Phe Ala Ala Trp Asp Pro Glu Lys Ala Ala 
        35                  40                  45              


Glu Glu Ala Lys Gly Arg Tyr Gly Gly Thr Leu Phe Pro Leu Asp Pro 
    50                  55                  60                  


Glu Arg Gly His Tyr Arg Leu Val Ala Gly Ala Leu Thr Leu Asp Phe 
65                  70                  75                  80  


Thr Pro Leu Glu Gly Ser Pro Glu Glu Asp Leu Leu Arg Arg Asp Tyr 
                85                  90                  95      


Arg Val Asn Ala Leu Leu Trp Leu Lys Gly Arg Val Phe Gly Leu Pro 
            100                 105                 110         


Gly Ala Glu Glu Asp Leu Asn Arg Arg Leu Leu Val Pro Val Gln Glu 
        115                 120                 125             


Glu Asn Leu Tyr Ala Asp Pro Leu Arg Ser Leu Arg Gly Val Arg Leu 
    130                 135                 140                 


Ala Ala Thr Leu Gly Leu Gly Leu Pro Pro Glu Thr Arg Arg Ala Leu 
145                 150                 155                 160 


Ala Arg His Ala Arg Phe Leu Gln Glu Asn Pro Ser Ala Leu Pro Ala 
                165                 170                 175     


Arg Glu Arg Val Arg Glu Glu Leu Ala Lys Leu Leu Leu Cys Lys Arg 
            180                 185                 190         


Ala Pro Phe Gly Leu His Leu Leu Glu Arg Thr Gly Leu Leu Gly Ile 
        195                 200                 205             


Tyr Leu Pro Glu Leu Thr His Leu Val Gly Leu His Gln Gly Gly Val 
    210                 215                 220                 


His His Leu Pro Ala Trp Glu His Thr Leu Ser Val Leu Phe His Leu 
225                 230                 235                 240 


Leu Trp Leu Trp Pro Glu Ala Pro Leu Glu Ala Arg Leu Ala Ala Leu 
                245                 250                 255     


Tyr His Asp Ala Gly Lys Pro Leu Thr Arg Arg Tyr Asp Pro Glu Val 
            260                 265                 270         


Gly Arg Tyr Arg Phe Phe Gly His Ala Glu Val Gly Ala Glu Met Ala 
        275                 280                 285             


Lys Ala Ala Leu Phe Trp Leu Arg Phe Pro Lys Glu Val Val Glu Lys 
    290                 295                 300                 


Ala Ala His Leu Val Arg Arg His Met Asp Arg Pro Pro Glu Gly Thr 
305                 310                 315                 320 


Lys Ala Leu Arg Arg Phe Tyr Phe Lys Arg Arg Asp Leu Leu Pro Ala 
                325                 330                 335     


Leu Pro Tyr Leu Met Ala Ala Asp Arg Leu Ala Ala Lys Gly Val Glu 
            340                 345                 350         


Ala Glu Ala Trp Pro Leu Leu Glu Ala Tyr Arg Glu Ala Leu Lys Thr 
        355                 360                 365             


Pro Leu Pro Gln Glu Pro Leu Leu Ser Gly Glu Glu Val Met Ala Leu 
    370                 375                 380                 


Leu Gly Leu Arg Pro Gly Pro Leu Val Gly Glu Ala Leu Arg Ala Leu 
385                 390                 395                 400 


Leu Glu Ala Gln Ala Glu Gly Ser Val Ala Ser Gln Glu Glu Ala Arg 
                405                 410                 415     


Arg Phe Leu Leu Tyr Trp Arg Asp Gly Arg Thr Ala Gln Ala Thr Pro 
            420                 425                 430         


Ala Pro Asp His Pro Gly 
        435             


<210>  3
<211>  435
<212>  PRT
<213>  Meiothermus silvanus

<400>  3

Met Leu Val Ala Ile Arg Arg Val His Leu Ser Pro Phe Pro Phe Pro 
1               5                   10                  15      


Ala Gln Gly Tyr Leu Val Gly Gly Ala Val Arg Asp Leu Met Leu Gly 
            20                  25                  30          


Lys Arg Pro Lys Asp Leu Asp Phe Val Val Pro Asn Pro Glu Glu Ala 
        35                  40                  45              


Ala Arg Asp Leu Ala His Arg Leu Gly Gly Ser Ile Phe Pro Leu Asp 
    50                  55                  60                  


Glu Lys Arg Arg Phe Trp Arg Val Val Ala Pro Gly Thr Thr Tyr Asp 
65                  70                  75                  80  


Tyr Ala Pro Met Pro Gln Gly Leu Glu Val Asp Leu Leu Arg Arg Asp 
                85                  90                  95      


Phe Thr Leu Asn Ala Leu Ala Val Asn Glu Gln Gly Leu Val Leu Gly 
            100                 105                 110         


Leu Pro Ile Ala Arg Tyr Asp Leu Glu His Arg Leu Leu Arg Ala Ile 
        115                 120                 125             


Arg Lys Ala Asn Leu Trp Glu Asp Pro Leu Arg Ser Leu Arg Ala Val 
    130                 135                 140                 


Arg Ile Thr Leu Thr Ala Gly Leu Arg Leu Glu Pro His Thr Glu Gly 
145                 150                 155                 160 


Trp Ile Tyr Glu His Ala Gln Tyr Leu Arg Glu Ser Gly Ala Leu Pro 
                165                 170                 175     


Ala Trp Glu Arg Val Gly Glu Glu Leu Ser Gln Ile Leu Arg His Pro 
            180                 185                 190         


Ala Ala Ala Val Gly Phe Leu Arg Leu Glu Arg Ala Gly Leu Leu Glu 
        195                 200                 205             


Val Tyr Leu Pro Glu Leu Ala Ala Gly Ile Gly Val Glu Gln His Gly 
    210                 215                 220                 


Ala His His Leu Asp Val Trp Arg His Ser Leu Glu Val Leu Ala Gln 
225                 230                 235                 240 


Leu Thr Leu Leu Arg Pro Asp Ala His Leu Ala Leu Arg Trp Ala Ala 
                245                 250                 255     


Leu Leu His Asp Val Ala Lys Pro Gln Thr Arg Gln Trp Ser Pro Glu 
            260                 265                 270         


Lys Gly Arg Tyr Thr Phe Tyr Gly His Asp Ala Leu Gly Ala Glu Gln 
        275                 280                 285             


Ala Arg Gln Ile Leu Asn Arg Leu Arg Gln Pro Ser Glu Val Val Ala 
    290                 295                 300                 


Glu Ala Val Arg Leu Val Arg Leu His Met Gln His Pro Pro Gln Gly 
305                 310                 315                 320 


Gly Arg Glu Leu Arg Arg Phe Leu His Arg Arg Arg Glu Ala Leu Pro 
                325                 330                 335     


Glu Leu Ile Trp Leu Gln Leu Ala Asp Met Thr Gly Ile Leu Gly Tyr 
            340                 345                 350         


Arg Glu Arg Val Glu Glu Leu Arg Glu Gln Leu Gln Ala Ile Glu Ser 
        355                 360                 365             


Leu Ala Glu Gly Thr Pro Ser Pro Lys Pro Leu Leu Asp Gly Arg Glu 
    370                 375                 380                 


Val Met Arg Leu Leu Gly Leu Glu Pro Gly Pro Lys Val Gly Glu Ala 
385                 390                 395                 400 


Leu Ala Ala Leu Leu Glu Ala Gln Ala Ile Gly Glu Val Gln Ser Arg 
                405                 410                 415     


Thr Glu Ala Ala Ser Phe Leu Arg Asp Ala Phe Gly Glu Asp Lys Gly 
            420                 425                 430         


Arg Met Ser 
        435 


<210>  4
<211>  454
<212>  PRT
<213>  Shigella flexneri

<400>  4

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Cys Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Ser Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Cys 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Cys Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Thr 
    450                 


<210>  5
<211>  454
<212>  PRT
<213>  Shigella dysenteriae

<400>  5

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Cys Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Cys 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Lys Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Cys Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala 
    450                 


<210>  6
<211>  454
<212>  PRT
<213>  Citrobacter koseri

<400>  6

Met Leu Asn Arg Glu Glu Ser Glu Ala Glu Leu Ala Val Ala Arg Pro 
1               5                   10                  15      


His Met Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Asp Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Cys Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Asp Ser Asp Arg Ala Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Gln Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Asp Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Phe Glu Thr Tyr Lys Lys Leu Arg 
                245                 250                 255     


Glu Tyr Ser Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Ala Met Glu Arg Ile Ile Ala Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Asn Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Met Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Ala Tyr Tyr Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Glu Val Leu Asp Glu Ala Cys Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Asn Asn Ala Glu Leu Gln Arg Leu Ala Gln Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Asp Pro Ala Pro Arg Arg Arg His Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala 
    450                 


<210>  7
<211>  454
<212>  PRT
<213>  Salmonella bongori

<400>  7

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


His Met Thr Ile Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Asp Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Cys Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Ser Glu Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Gln Asp Leu 
                165                 170                 175     


Lys Glu Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Asn Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Lys Leu Arg 
                245                 250                 255     


Glu Tyr Asn Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Ala Met Glu Arg Ile Ile Ala Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Glu Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Met Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Ala Tyr Tyr Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Glu Val Leu Asp Glu Ala Cys Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Met Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Phe Asp Leu Leu Glu Leu Arg Ala Gln Val Glu 
385                 390                 395                 400 


Asn Asn Ala Glu Leu Gln Arg Leu Ala Lys Trp Trp Ser Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Glu Gln Lys Gly Met Leu Asn Glu Leu Asp Asp 
            420                 425                 430         


Asp Pro Ala Pro Arg Arg Arg Arg Pro Arg Pro Arg Arg Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Ala Ala 
    450                 


<210>  8
<211>  443
<212>  PRT
<213>  Salmonella enterica

<400>  8

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


His Met Thr Ile Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Leu Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Tyr Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Asp Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Cys Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Ser Glu Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Gln Asp Leu 
                165                 170                 175     


Gln Glu Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Asn Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Asn Gly Tyr Glu Thr Tyr Gln Gln Leu Arg 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Ala Met Glu Arg Ile Ile Ala Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Asn Arg Ile Arg Asn Glu Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Met Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Ala Tyr Tyr Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Cys Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Met Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Phe Asp Leu Leu Glu Leu Arg Ala Gln Val Glu 
385                 390                 395                 400 


Asn Asn Thr Glu Leu Gln Arg Leu Ala Gln Trp Trp Ala Glu Phe Gln 
                405                 410                 415     


Ala Ser Ala Pro Pro Glu Gln Lys Gly Met Leu Asn Glu Leu Asp Asp 
            420                 425                 430         


Asp Pro Ala Pro Arg Arg Arg Arg Ser Arg Pro 
        435                 440             


<210>  9
<211>  453
<212>  PRT
<213>  Trabulsiella guamensis

<400>  9

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Ile Val Val Ala Arg Pro 
1               5                   10                  15      


Lys Met Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Leu Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Tyr Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Ser Ala Thr Pro Asp Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Cys Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Ser Ser Ile Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Gln Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Ile Asn Asp Val Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Phe Glu Thr Trp Asn Leu Leu Arg 
                245                 250                 255     


Glu Tyr Ser Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Ala Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Glu Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Met Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Ala Tyr Tyr Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Thr Asn Asp Val Leu Asp Glu Ala Cys Arg Ser Ile Ala Ile Pro 
            340                 345                 350         


Lys Arg Ile Thr Thr Leu Val Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Met Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ser Leu Arg Ala Glu Ala Glu 
385                 390                 395                 400 


His Asn Ala Glu Leu Gln Arg Leu Thr Gln Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ala Ala Pro Pro Glu Gln Lys Asp Met Leu Thr Asp Leu Gly Asp 
            420                 425                 430         


Glu Pro Ala Arg Arg Arg His Arg Arg Pro Arg Lys Arg Thr Pro Arg 
        435                 440                 445             


Pro Gly Ser Pro Ala 
    450             


<210>  10
<211>  454
<212>  PRT
<213>  Kluyvera ascorbata

<400>  10

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Asn Ala Val Ala Lys Pro 
1               5                   10                  15      


Ala Met Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Leu Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Tyr Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Ser Ala Thr Pro Asp Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Cys Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Asp Gly Gln Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Gln Asp Leu 
                165                 170                 175     


Ile Asp Gly Val Ile Arg Leu Ile Gly Asp Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Asp Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Met Asn Asp Val Pro Pro Ala Arg Leu Phe Glu Glu Ala Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Phe Glu Thr Tyr Lys Leu Leu Arg 
                245                 250                 255     


Glu Tyr Asn Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asp Gly Asp Ser Pro Met Glu Arg Ile Ile Ser Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile Arg Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Met Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Ala Tyr Tyr Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Glu Val Leu Asp Glu Ala Cys Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Ile Thr Thr Leu Val Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Met Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Ala Glu 
385                 390                 395                 400 


His Asn Gly Glu Leu Gln Arg Leu Thr Gln Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Val Ala Pro Pro Ala Gln Lys Asp Met Leu Asn Glu Leu Gly Asp 
            420                 425                 430         


Glu Pro Asp Thr Arg Arg Arg His Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Ser Ser 
    450                 


<210>  11
<211>  436
<212>  PRT
<213>  Citrobacter freundii

<400>  11

Met Thr Val Ile Pro Arg Glu Gln His Ser Ile Ser Arg Lys Asp Ile 
1               5                   10                  15      


Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly Tyr 
            20                  25                  30          


Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly Lys 
        35                  40                  45              


Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Asp Gln Val 
    50                  55                  60                  


Arg Lys Leu Phe Arg Asn Cys Arg Leu Val Gly Arg Arg Phe Arg Leu 
65                  70                  75                  80  


Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe Arg 
                85                  90                  95      


Gly His His Glu Gly Ser Glu Ala Asp Arg Thr Thr Ser Gln Arg Gly 
            100                 105                 110         


Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu Glu 
        115                 120                 125             


Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser Val 
    130                 135                 140                 


Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Gln Asp Leu Glu 
145                 150                 155                 160 


Glu Gly Ile Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg Glu 
                165                 170                 175     


Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu Asp 
            180                 185                 190         


Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala Thr 
        195                 200                 205             


Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ala Leu Lys 
    210                 215                 220                 


Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Lys Leu Arg Glu 
225                 230                 235                 240 


Tyr Ser Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe Thr 
                245                 250                 255     


Glu Asp Gly Asp Ser Pro Met Glu Arg Ile Ile Ala Gln Val Leu Lys 
            260                 265                 270         


Asn Thr Asp Asn Arg Ile Asn Asn Asp Met Arg Val Asn Pro Ala Phe 
        275                 280                 285             


Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Ala Ala Gln Lys 
    290                 295                 300                 


Ile Ala Gln Glu Ser Gly Leu Ala Tyr Tyr Asp Ala Phe Ala Leu Ala 
305                 310                 315                 320 


Met Asn Asp Val Leu Asp Glu Ala Cys Arg Ser Leu Ala Ile Pro Lys 
                325                 330                 335     


Arg Leu Thr Ser Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg Met 
            340                 345                 350         


Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Met Glu His Pro Lys 
        355                 360                 365             


Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu Asn 
    370                 375                 380                 


Asn Ala Glu Leu Gln Arg Leu Ala Gln Trp Trp Gly Glu Phe Gln Val 
385                 390                 395                 400 


Ser Ala Pro Pro Glu Gln Lys Gly Met Leu Asn Glu Leu Asp Glu Glu 
                405                 410                 415     


Pro Glu Arg Arg Arg His Arg Arg Pro Arg Lys Arg Ala Pro Arg Arg 
            420                 425                 430         


Glu Gly Ser Ala 
        435     


<210>  12
<211>  447
<212>  PRT
<213>  Enterobacter cloacae

<400>  12

Met Ala Asn Asp Ala Ile Ala Gln Pro His Met Ser Val Ile Pro Arg 
1               5                   10                  15      


Glu Gln His Thr Ile Ser Arg Lys Asp Ile Ser Glu Asn Ala Leu Lys 
            20                  25                  30          


Val Leu Tyr Arg Leu Asn Lys Ala Gly Tyr Glu Ala Tyr Leu Val Gly 
        35                  40                  45              


Gly Gly Val Arg Asp Leu Leu Leu Gly Lys Lys Pro Lys Asp Phe Asp 
    50                  55                  60                  


Val Thr Thr Ser Ala Thr Pro Glu Gln Val Arg Lys Leu Phe Arg Asn 
65                  70                  75                  80  


Cys Arg Leu Val Gly Arg Arg Phe Arg Leu Ala His Val Met Phe Gly 
                85                  90                  95      


Pro Glu Ile Ile Glu Val Ala Thr Phe Arg Gly His His Glu Ala Gly 
            100                 105                 110         


Ala Ser Asp Arg Thr Thr Ser Gln Arg Gly Gln Asn Gly Met Leu Leu 
        115                 120                 125             


Arg Asp Asn Ile Phe Gly Ser Ile Glu Glu Asp Ala Gln Arg Arg Asp 
    130                 135                 140                 


Phe Thr Ile Asn Ser Leu Tyr Tyr Ser Val Ala Asp Phe Thr Val Arg 
145                 150                 155                 160 


Asp Tyr Val Gly Gly Met Gln Asp Leu Lys Glu Gly Leu Ile Arg Leu 
                165                 170                 175     


Ile Gly Thr Pro Glu Thr Arg Tyr Arg Glu Asp Pro Val Arg Met Leu 
            180                 185                 190         


Arg Ala Val Arg Phe Ala Ala Lys Leu Asn Met Arg Ile Ser Pro Glu 
        195                 200                 205             


Thr Ala Glu Pro Ile Pro Arg Leu Ala Thr Leu Ile Asn Asp Val Pro 
    210                 215                 220                 


Pro Ala Arg Leu Phe Glu Glu Ala Leu Lys Leu Leu Gln Ala Gly His 
225                 230                 235                 240 


Gly Phe Asp Thr Tyr Asn Leu Leu Arg Glu Tyr Asn Leu Phe Gln Pro 
                245                 250                 255     


Leu Phe Pro Thr Ile Thr Arg Tyr Phe Thr Glu Asn Gly Asp Ser Pro 
            260                 265                 270         


Met Glu Arg Met Ile Ala Gln Val Leu Lys Asn Thr Asp Thr Arg Ile 
        275                 280                 285             


Arg Asn Asp Met Arg Val Asn Pro Ala Phe Leu Phe Ala Ala Met Phe 
    290                 295                 300                 


Trp Tyr Pro Leu Leu Glu Thr Ala Gln Arg Ile Thr Gln Glu Ser Gly 
305                 310                 315                 320 


Leu Ala Tyr Tyr Asp Ala Phe Ala Leu Ala Ala Asn Asp Val Leu Asp 
                325                 330                 335     


Glu Gly Cys Arg Thr Leu Ala Ile Pro Lys Arg Ile Thr Thr Leu Val 
            340                 345                 350         


Arg Asp Ile Trp Gln Leu Gln Leu Arg Met Ser Arg Arg Gln Gly Lys 
        355                 360                 365             


Arg Ala Trp Lys Leu Met Glu His Pro Lys Phe Arg Ala Ala Phe Asp 
    370                 375                 380                 


Leu Leu Ser Leu Arg Ala Glu Ile Glu Arg Asn Gln Glu Leu Gln Arg 
385                 390                 395                 400 


Leu Ser Gln Trp Trp Ala Glu Phe Gln Val Ser Ala Pro Pro Glu Gln 
                405                 410                 415     


Lys Asp Met Leu Thr Gly Leu Asp Glu Glu Pro Glu Pro Arg Arg Arg 
            420                 425                 430         


His Arg Arg Pro Arg Lys Arg Ala Pro Arg Arg Glu Gly Thr Ala 
        435                 440                 445         


<210>  13
<211>  436
<212>  PRT
<213>  Enterococcus gallinarum

<400>  13

Met Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp Ile 
1               5                   10                  15      


Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly Tyr 
            20                  25                  30          


Glu Ser Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly Lys 
        35                  40                  45              


Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Asp Gln Val 
    50                  55                  60                  


Arg Lys Leu Phe Arg Asn Cys Arg Leu Val Gly Arg Arg Phe Arg Leu 
65                  70                  75                  80  


Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe Arg 
                85                  90                  95      


Gly His His Glu Gly His Thr Thr Asp Arg Val Thr Ser Gln Arg Gly 
            100                 105                 110         


Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu Glu 
        115                 120                 125             


Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser Val 
    130                 135                 140                 


Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu Gln 
145                 150                 155                 160 


Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg Glu 
                165                 170                 175     


Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu Asn 
            180                 185                 190         


Met Thr Ile Ser Pro Glu Thr Ala Glu Pro Leu Pro Arg Leu Ala Ala 
        195                 200                 205             


Leu Leu His Asp Val Pro Pro Ala Arg Leu Phe Glu Glu Val Leu Lys 
    210                 215                 220                 


Leu Leu Gln Ala Gly Tyr Gly Tyr Gln Thr Tyr Met Leu Leu Arg Glu 
225                 230                 235                 240 


Tyr Ser Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe Thr 
                245                 250                 255     


Glu Arg Gly Asp Ser Pro Met Glu Arg Ile Ile Ser Gln Val Leu Lys 
            260                 265                 270         


Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala Phe 
        275                 280                 285             


Leu Phe Ala Val Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln Lys 
    290                 295                 300                 


Ile Ala Gln Glu Ser Gly Leu Ala Tyr Tyr Asp Ala Phe Ala Leu Ala 
305                 310                 315                 320 


Met Asn Asp Val Leu Asp Glu Ala Cys Arg Thr Leu Ala Ile Pro Lys 
                325                 330                 335     


Arg Ile Thr Thr Leu Ile Arg Asp Ile Trp Gln Leu Gln Leu Arg Met 
            340                 345                 350         


Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Met Glu His Pro Lys 
        355                 360                 365             


Phe Arg Ala Ala Tyr Asp Leu Leu Glu Leu Arg Ala Gly Ala Glu Asn 
    370                 375                 380                 


Asn His Glu Leu Gln Arg Leu Thr Lys Trp Trp Gly Glu Phe Gln Val 
385                 390                 395                 400 


Ala Ala Pro Pro Ala Gln Lys Asp Met Leu Asn Asp Leu Gly Asp Asp 
                405                 410                 415     


Pro Ala Pro Arg Arg Arg His Arg Arg Pro Arg Lys Arg Ala Pro Arg 
            420                 425                 430         


Gln Gly Ser Ala 
        435     


<210>  14
<211>  455
<212>  PRT
<213>  Grimontia indica

<400>  14

Met Asn Arg Asp Asn Lys Thr Arg Ala Ile Asp Asp Leu Lys Leu Glu 
1               5                   10                  15      


Val Ile Pro Arg Gln Glu His Gly Ile Ser Arg Lys Asp Ile Ser Glu 
            20                  25                  30          


Asn Ala Leu Lys Val Leu Tyr Arg Leu Asn Lys Ala Gly Phe Glu Ala 
        35                  40                  45              


Tyr Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Asn Lys Gln Pro 
    50                  55                  60                  


Lys Asp Phe Asp Ile Ala Thr Asn Ala Thr Pro Glu Glu Val Arg Lys 
65                  70                  75                  80  


Leu Phe Arg Asn Cys Arg Leu Val Gly Arg Arg Phe Arg Leu Ala His 
                85                  90                  95      


Ile Leu Phe Gly Arg Asp Val Ile Glu Val Ala Thr Phe Arg Gly His 
            100                 105                 110         


His Gly Ala Asp Lys Gly Asp Lys Ser Ala Ser Gln Ser Asn Asp Gly 
        115                 120                 125             


Met Leu Leu Arg Asp Asn Val Tyr Gly Thr Ile Asp Glu Asp Ala Glu 
    130                 135                 140                 


Arg Arg Asp Phe Thr Val Asn Ala Leu Tyr Tyr Asn Ile Asp Asp Phe 
145                 150                 155                 160 


Ser Val Arg Asp Tyr Ala Gly Gly Val Glu Asp Leu Lys Asn Arg Val 
                165                 170                 175     


Ile Arg Leu Ile Gly Asp Pro Glu Thr Arg Tyr Arg Glu Asp Pro Val 
            180                 185                 190         


Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu Asp Met Gln Ile 
        195                 200                 205             


Asn Glu Ala Thr Ala Ala Pro Ile Pro Ala Leu Ala His Leu Leu Arg 
    210                 215                 220                 


Asp Ile Pro Ala Ala Arg Leu Phe Glu Glu Ser Leu Lys Leu Leu Gln 
225                 230                 235                 240 


Ser Gly Asn Gly Leu Asp Thr Tyr Arg Leu Leu Arg Asp Tyr Asn Leu 
                245                 250                 255     


Phe Ser Pro Leu Phe Pro Ile Leu Asp Glu His Phe Thr Glu Asp Arg 
            260                 265                 270         


Ser Ser Lys Cys Glu Lys Met Leu Glu Leu Val Leu Lys Ser Thr Asp 
        275                 280                 285             


Asp Arg Val Ala Asn Asp Gln Arg Ile Asn Pro Ala Phe Met Tyr Ala 
    290                 295                 300                 


Ala Met Leu Trp Tyr Pro Leu Glu Thr Arg Ala Glu Glu Ile Ala Phe 
305                 310                 315                 320 


Glu Ser Gly Leu Ser Tyr Tyr Asp Ala Phe Met Val Ala Ala Asn Asp 
                325                 330                 335     


Ile Leu Asp Glu Gln Val Lys Ser Ile Ala Ile Pro Arg Arg Phe Thr 
            340                 345                 350         


Ala Met Ile Arg Asp Ile Trp Gln Leu Gln Leu Arg Leu Ser Arg Arg 
        355                 360                 365             


Ser Gly Asn Arg Ala Tyr Lys Leu Leu Glu Gln Asn Lys Phe Arg Ala 
    370                 375                 380                 


Ala Phe Asp Phe Leu Glu Met Arg Gly Lys Ile Glu Gly Gly Gln Leu 
385                 390                 395                 400 


Glu Glu Leu Ala Thr Trp Trp Asp Asp Phe Gln Arg Ala Asn Arg Asp 
                405                 410                 415     


Asp Arg His Asp Met Val Gln Val Leu Asn Lys Ala Ser Ala Ala Lys 
            420                 425                 430         


Lys Thr Arg Arg Arg Arg Lys Thr Lys Pro Arg Ser Arg Lys Pro Lys 
        435                 440                 445             


Pro Lys Ser Asn Thr Glu Ser 
    450                 455 


<210>  15
<211>  457
<212>  PRT
<213>  Salinivibrio costicola

<400>  15

Met Asn Arg Asp Gly Lys Thr Arg Ala Ile Glu Asp Leu Lys Leu Glu 
1               5                   10                  15      


Val Ile Pro Arg Lys Ala His Gly Ile Ser Arg Lys Gly Ile Ser Glu 
            20                  25                  30          


Asn Ala Leu Lys Val Leu Tyr Arg Leu His Lys Ala Gly Tyr Glu Ala 
        35                  40                  45              


Tyr Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Asp Arg Glu Pro 
    50                  55                  60                  


Lys Asp Phe Asp Ile Ala Thr Asn Ala Thr Pro Asp Glu Val Arg Lys 
65                  70                  75                  80  


Leu Phe Arg Asn Cys Arg Leu Ile Gly Arg Arg Phe Arg Leu Ala His 
                85                  90                  95      


Ile Leu Phe Gly Arg Asp Val Ile Glu Val Ala Thr Phe Arg Gly His 
            100                 105                 110         


His Gly Lys Ser Glu Pro Asp Lys Lys Asp Lys Gln Thr Ala Arg Ser 
        115                 120                 125             


Glu Asp Gly Met Leu Leu Arg Asp Asn Val Tyr Gly Thr Ile Asp Glu 
    130                 135                 140                 


Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Gly Leu Tyr Tyr Asn Ile 
145                 150                 155                 160 


Ala Asp Phe Ser Ile Arg Asp Tyr Ala Gly Gly Leu Asp Asp Leu Asn 
                165                 170                 175     


Asn Arg Val Val Arg Leu Ile Gly Asp Pro Glu Thr Arg Tyr Arg Glu 
            180                 185                 190         


Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Val Lys Leu Asp 
        195                 200                 205             


Met Thr Ile Asn Asp Ala Thr Ala Ala Pro Met Val Glu Leu Ala Pro 
    210                 215                 220                 


Leu Leu Gln Asp Ile Pro Ala Ala Arg Met Phe Glu Glu Ser Leu Lys 
225                 230                 235                 240 


Leu Leu Gln Ala Gly Gln Gly Leu Ala Thr Tyr Gln Met Leu Arg Glu 
                245                 250                 255     


Tyr Asn Leu Phe Gln Gln Leu Phe Pro Ala Val Ala Ala His Phe Thr 
            260                 265                 270         


Glu Ala Gln Thr Ser Gln Thr Glu Gln Met Leu Glu Gln Val Leu Arg 
        275                 280                 285             


Ser Thr Asp Lys Arg Val Ala Asn Asp Gln Arg Ile Asn Pro Ala Phe 
    290                 295                 300                 


Leu Phe Ala Ala Met Leu Trp Tyr Pro Leu Val Thr Arg Ala Glu Glu 
305                 310                 315                 320 


Met Ala Phe Glu Ser Gly Leu Ser Tyr Tyr Asp Ala Phe Met Val Ala 
                325                 330                 335     


Ala Ser Asp Ile Val Asp Glu Gln Val Lys Thr Val Ala Ile Pro Arg 
            340                 345                 350         


Arg Phe Thr Ala Met Val Arg Glu Val Trp Gln Leu Gln Met Arg Met 
        355                 360                 365             


Thr Arg Arg Thr Gly Ala Arg Ala Phe Lys Thr Leu Glu Glu Lys Lys 
    370                 375                 380                 


Phe Arg Ala Ala Phe Asp Leu Leu Glu Met Arg Gly Ala Val Glu Gly 
385                 390                 395                 400 


Asp Asp Thr Glu Ala Leu Cys Ala Trp Trp Arg Asp Phe Gln Arg Ala 
                405                 410                 415     


Asp Gln Gly Gly Arg Gln Lys Met Val Arg Ala Ile Asn Lys Val Ser 
            420                 425                 430         


Asn Thr Arg Ser Pro Lys Ser Arg Arg Arg Lys Gly Pro Pro Arg Arg 
        435                 440                 445             


Arg Lys Pro Gln Ser Asn Ser Asp Ala 
    450                 455         


<210>  16
<211>  454
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256A

<400>  16

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Cys Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala 
    450                 


<210>  17
<211>  455
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256A C346A 455C

<400>  17

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Cys 
    450                 455 


<210>  18
<211>  461
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256A C346A 455 (6xG)C

<400>  18

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Gly Gly Cys 
    450                 455                 460     


<210>  19
<211>  439
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Thermus aquaticus poly(A) polymerase C190A 439C

<400>  19

Met Leu Arg Leu Ile Ala His Arg Asp Phe Pro Phe Tyr Thr Pro Lys 
1               5                   10                  15      


Gly Ala Ile Pro Val Gly Gly Ala Leu Arg Asp Leu Leu Leu Gly Glu 
            20                  25                  30          


Arg Pro Leu Asp Leu Asp Phe Ala Ala Trp Asp Pro Glu Lys Ala Ala 
        35                  40                  45              


Glu Glu Ala Lys Gly Arg Tyr Gly Gly Thr Leu Phe Pro Leu Asp Pro 
    50                  55                  60                  


Glu Arg Gly His Tyr Arg Leu Val Ala Gly Ala Leu Thr Leu Asp Phe 
65                  70                  75                  80  


Thr Pro Leu Glu Gly Ser Pro Glu Glu Asp Leu Leu Arg Arg Asp Tyr 
                85                  90                  95      


Arg Val Asn Ala Leu Leu Trp Leu Lys Gly Arg Val Phe Gly Leu Pro 
            100                 105                 110         


Gly Ala Glu Glu Asp Leu Asn Arg Arg Leu Leu Val Pro Val Gln Glu 
        115                 120                 125             


Glu Asn Leu Tyr Ala Asp Pro Leu Arg Ser Leu Arg Gly Val Arg Leu 
    130                 135                 140                 


Ala Ala Thr Leu Gly Leu Gly Leu Pro Pro Glu Thr Arg Arg Ala Leu 
145                 150                 155                 160 


Ala Arg His Ala Arg Phe Leu Gln Glu Asn Pro Ser Ala Leu Pro Ala 
                165                 170                 175     


Arg Glu Arg Val Arg Glu Glu Leu Ala Lys Leu Leu Leu Ala Lys Arg 
            180                 185                 190         


Ala Pro Phe Gly Leu His Leu Leu Glu Arg Thr Gly Leu Leu Gly Ile 
        195                 200                 205             


Tyr Leu Pro Glu Leu Thr His Leu Val Gly Leu His Gln Gly Gly Val 
    210                 215                 220                 


His His Leu Pro Ala Trp Glu His Thr Leu Ser Val Leu Phe His Leu 
225                 230                 235                 240 


Leu Trp Leu Trp Pro Glu Ala Pro Leu Glu Ala Arg Leu Ala Ala Leu 
                245                 250                 255     


Tyr His Asp Ala Gly Lys Pro Leu Thr Arg Arg Tyr Asp Pro Glu Val 
            260                 265                 270         


Gly Arg Tyr Arg Phe Phe Gly His Ala Glu Val Gly Ala Glu Met Ala 
        275                 280                 285             


Lys Ala Ala Leu Phe Trp Leu Arg Phe Pro Lys Glu Val Val Glu Lys 
    290                 295                 300                 


Ala Ala His Leu Val Arg Arg His Met Asp Arg Pro Pro Glu Gly Thr 
305                 310                 315                 320 


Lys Ala Leu Arg Arg Phe Tyr Phe Lys Arg Arg Asp Leu Leu Pro Ala 
                325                 330                 335     


Leu Pro Tyr Leu Met Ala Ala Asp Arg Leu Ala Ala Lys Gly Val Glu 
            340                 345                 350         


Ala Glu Ala Trp Pro Leu Leu Glu Ala Tyr Arg Glu Ala Leu Lys Thr 
        355                 360                 365             


Pro Leu Pro Gln Glu Pro Leu Leu Ser Gly Glu Glu Val Met Ala Leu 
    370                 375                 380                 


Leu Gly Leu Arg Pro Gly Pro Leu Val Gly Glu Ala Leu Arg Ala Leu 
385                 390                 395                 400 


Leu Glu Ala Gln Ala Glu Gly Ser Val Ala Ser Gln Glu Glu Ala Arg 
                405                 410                 415     


Arg Phe Leu Leu Tyr Trp Arg Asp Gly Arg Thr Ala Gln Ala Thr Pro 
            420                 425                 430         


Ala Pro Asp His Pro Gly Cys 
        435                 


<210>  20
<211>  445
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Thermus aquaticus poly(A) polymerase C190A 439(6xG)C

<400>  20

Met Leu Arg Leu Ile Ala His Arg Asp Phe Pro Phe Tyr Thr Pro Lys 
1               5                   10                  15      


Gly Ala Ile Pro Val Gly Gly Ala Leu Arg Asp Leu Leu Leu Gly Glu 
            20                  25                  30          


Arg Pro Leu Asp Leu Asp Phe Ala Ala Trp Asp Pro Glu Lys Ala Ala 
        35                  40                  45              


Glu Glu Ala Lys Gly Arg Tyr Gly Gly Thr Leu Phe Pro Leu Asp Pro 
    50                  55                  60                  


Glu Arg Gly His Tyr Arg Leu Val Ala Gly Ala Leu Thr Leu Asp Phe 
65                  70                  75                  80  


Thr Pro Leu Glu Gly Ser Pro Glu Glu Asp Leu Leu Arg Arg Asp Tyr 
                85                  90                  95      


Arg Val Asn Ala Leu Leu Trp Leu Lys Gly Arg Val Phe Gly Leu Pro 
            100                 105                 110         


Gly Ala Glu Glu Asp Leu Asn Arg Arg Leu Leu Val Pro Val Gln Glu 
        115                 120                 125             


Glu Asn Leu Tyr Ala Asp Pro Leu Arg Ser Leu Arg Gly Val Arg Leu 
    130                 135                 140                 


Ala Ala Thr Leu Gly Leu Gly Leu Pro Pro Glu Thr Arg Arg Ala Leu 
145                 150                 155                 160 


Ala Arg His Ala Arg Phe Leu Gln Glu Asn Pro Ser Ala Leu Pro Ala 
                165                 170                 175     


Arg Glu Arg Val Arg Glu Glu Leu Ala Lys Leu Leu Leu Ala Lys Arg 
            180                 185                 190         


Ala Pro Phe Gly Leu His Leu Leu Glu Arg Thr Gly Leu Leu Gly Ile 
        195                 200                 205             


Tyr Leu Pro Glu Leu Thr His Leu Val Gly Leu His Gln Gly Gly Val 
    210                 215                 220                 


His His Leu Pro Ala Trp Glu His Thr Leu Ser Val Leu Phe His Leu 
225                 230                 235                 240 


Leu Trp Leu Trp Pro Glu Ala Pro Leu Glu Ala Arg Leu Ala Ala Leu 
                245                 250                 255     


Tyr His Asp Ala Gly Lys Pro Leu Thr Arg Arg Tyr Asp Pro Glu Val 
            260                 265                 270         


Gly Arg Tyr Arg Phe Phe Gly His Ala Glu Val Gly Ala Glu Met Ala 
        275                 280                 285             


Lys Ala Ala Leu Phe Trp Leu Arg Phe Pro Lys Glu Val Val Glu Lys 
    290                 295                 300                 


Ala Ala His Leu Val Arg Arg His Met Asp Arg Pro Pro Glu Gly Thr 
305                 310                 315                 320 


Lys Ala Leu Arg Arg Phe Tyr Phe Lys Arg Arg Asp Leu Leu Pro Ala 
                325                 330                 335     


Leu Pro Tyr Leu Met Ala Ala Asp Arg Leu Ala Ala Lys Gly Val Glu 
            340                 345                 350         


Ala Glu Ala Trp Pro Leu Leu Glu Ala Tyr Arg Glu Ala Leu Lys Thr 
        355                 360                 365             


Pro Leu Pro Gln Glu Pro Leu Leu Ser Gly Glu Glu Val Met Ala Leu 
    370                 375                 380                 


Leu Gly Leu Arg Pro Gly Pro Leu Val Gly Glu Ala Leu Arg Ala Leu 
385                 390                 395                 400 


Leu Glu Ala Gln Ala Glu Gly Ser Val Ala Ser Gln Glu Glu Ala Arg 
                405                 410                 415     


Arg Phe Leu Leu Tyr Trp Arg Asp Gly Arg Thr Ala Gln Ala Thr Pro 
            420                 425                 430         


Ala Pro Asp His Pro Gly Gly Gly Gly Gly Gly Gly Cys 
        435                 440                 445 


<210>  21
<211>  436
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Meiothermus silvanus polynucleotide adenylyltransferase 436C

<400>  21

Met Leu Val Ala Ile Arg Arg Val His Leu Ser Pro Phe Pro Phe Pro 
1               5                   10                  15      


Ala Gln Gly Tyr Leu Val Gly Gly Ala Val Arg Asp Leu Met Leu Gly 
            20                  25                  30          


Lys Arg Pro Lys Asp Leu Asp Phe Val Val Pro Asn Pro Glu Glu Ala 
        35                  40                  45              


Ala Arg Asp Leu Ala His Arg Leu Gly Gly Ser Ile Phe Pro Leu Asp 
    50                  55                  60                  


Glu Lys Arg Arg Phe Trp Arg Val Val Ala Pro Gly Thr Thr Tyr Asp 
65                  70                  75                  80  


Tyr Ala Pro Met Pro Gln Gly Leu Glu Val Asp Leu Leu Arg Arg Asp 
                85                  90                  95      


Phe Thr Leu Asn Ala Leu Ala Val Asn Glu Gln Gly Leu Val Leu Gly 
            100                 105                 110         


Leu Pro Ile Ala Arg Tyr Asp Leu Glu His Arg Leu Leu Arg Ala Ile 
        115                 120                 125             


Arg Lys Ala Asn Leu Trp Glu Asp Pro Leu Arg Ser Leu Arg Ala Val 
    130                 135                 140                 


Arg Ile Thr Leu Thr Ala Gly Leu Arg Leu Glu Pro His Thr Glu Gly 
145                 150                 155                 160 


Trp Ile Tyr Glu His Ala Gln Tyr Leu Arg Glu Ser Gly Ala Leu Pro 
                165                 170                 175     


Ala Trp Glu Arg Val Gly Glu Glu Leu Ser Gln Ile Leu Arg His Pro 
            180                 185                 190         


Ala Ala Ala Val Gly Phe Leu Arg Leu Glu Arg Ala Gly Leu Leu Glu 
        195                 200                 205             


Val Tyr Leu Pro Glu Leu Ala Ala Gly Ile Gly Val Glu Gln His Gly 
    210                 215                 220                 


Ala His His Leu Asp Val Trp Arg His Ser Leu Glu Val Leu Ala Gln 
225                 230                 235                 240 


Leu Thr Leu Leu Arg Pro Asp Ala His Leu Ala Leu Arg Trp Ala Ala 
                245                 250                 255     


Leu Leu His Asp Val Ala Lys Pro Gln Thr Arg Gln Trp Ser Pro Glu 
            260                 265                 270         


Lys Gly Arg Tyr Thr Phe Tyr Gly His Asp Ala Leu Gly Ala Glu Gln 
        275                 280                 285             


Ala Arg Gln Ile Leu Asn Arg Leu Arg Gln Pro Ser Glu Val Val Ala 
    290                 295                 300                 


Glu Ala Val Arg Leu Val Arg Leu His Met Gln His Pro Pro Gln Gly 
305                 310                 315                 320 


Gly Arg Glu Leu Arg Arg Phe Leu His Arg Arg Arg Glu Ala Leu Pro 
                325                 330                 335     


Glu Leu Ile Trp Leu Gln Leu Ala Asp Met Thr Gly Ile Leu Gly Tyr 
            340                 345                 350         


Arg Glu Arg Val Glu Glu Leu Arg Glu Gln Leu Gln Ala Ile Glu Ser 
        355                 360                 365             


Leu Ala Glu Gly Thr Pro Ser Pro Lys Pro Leu Leu Asp Gly Arg Glu 
    370                 375                 380                 


Val Met Arg Leu Leu Gly Leu Glu Pro Gly Pro Lys Val Gly Glu Ala 
385                 390                 395                 400 


Leu Ala Ala Leu Leu Glu Ala Gln Ala Ile Gly Glu Val Gln Ser Arg 
                405                 410                 415     


Thr Glu Ala Ala Ser Phe Leu Arg Asp Ala Phe Gly Glu Asp Lys Gly 
            420                 425                 430         


Arg Met Ser Cys 
        435     


<210>  22
<211>  442
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Meiothermus silvanus polynucleotide adenylyltransferase 436 
       (6xG)C

<400>  22

Met Leu Val Ala Ile Arg Arg Val His Leu Ser Pro Phe Pro Phe Pro 
1               5                   10                  15      


Ala Gln Gly Tyr Leu Val Gly Gly Ala Val Arg Asp Leu Met Leu Gly 
            20                  25                  30          


Lys Arg Pro Lys Asp Leu Asp Phe Val Val Pro Asn Pro Glu Glu Ala 
        35                  40                  45              


Ala Arg Asp Leu Ala His Arg Leu Gly Gly Ser Ile Phe Pro Leu Asp 
    50                  55                  60                  


Glu Lys Arg Arg Phe Trp Arg Val Val Ala Pro Gly Thr Thr Tyr Asp 
65                  70                  75                  80  


Tyr Ala Pro Met Pro Gln Gly Leu Glu Val Asp Leu Leu Arg Arg Asp 
                85                  90                  95      


Phe Thr Leu Asn Ala Leu Ala Val Asn Glu Gln Gly Leu Val Leu Gly 
            100                 105                 110         


Leu Pro Ile Ala Arg Tyr Asp Leu Glu His Arg Leu Leu Arg Ala Ile 
        115                 120                 125             


Arg Lys Ala Asn Leu Trp Glu Asp Pro Leu Arg Ser Leu Arg Ala Val 
    130                 135                 140                 


Arg Ile Thr Leu Thr Ala Gly Leu Arg Leu Glu Pro His Thr Glu Gly 
145                 150                 155                 160 


Trp Ile Tyr Glu His Ala Gln Tyr Leu Arg Glu Ser Gly Ala Leu Pro 
                165                 170                 175     


Ala Trp Glu Arg Val Gly Glu Glu Leu Ser Gln Ile Leu Arg His Pro 
            180                 185                 190         


Ala Ala Ala Val Gly Phe Leu Arg Leu Glu Arg Ala Gly Leu Leu Glu 
        195                 200                 205             


Val Tyr Leu Pro Glu Leu Ala Ala Gly Ile Gly Val Glu Gln His Gly 
    210                 215                 220                 


Ala His His Leu Asp Val Trp Arg His Ser Leu Glu Val Leu Ala Gln 
225                 230                 235                 240 


Leu Thr Leu Leu Arg Pro Asp Ala His Leu Ala Leu Arg Trp Ala Ala 
                245                 250                 255     


Leu Leu His Asp Val Ala Lys Pro Gln Thr Arg Gln Trp Ser Pro Glu 
            260                 265                 270         


Lys Gly Arg Tyr Thr Phe Tyr Gly His Asp Ala Leu Gly Ala Glu Gln 
        275                 280                 285             


Ala Arg Gln Ile Leu Asn Arg Leu Arg Gln Pro Ser Glu Val Val Ala 
    290                 295                 300                 


Glu Ala Val Arg Leu Val Arg Leu His Met Gln His Pro Pro Gln Gly 
305                 310                 315                 320 


Gly Arg Glu Leu Arg Arg Phe Leu His Arg Arg Arg Glu Ala Leu Pro 
                325                 330                 335     


Glu Leu Ile Trp Leu Gln Leu Ala Asp Met Thr Gly Ile Leu Gly Tyr 
            340                 345                 350         


Arg Glu Arg Val Glu Glu Leu Arg Glu Gln Leu Gln Ala Ile Glu Ser 
        355                 360                 365             


Leu Ala Glu Gly Thr Pro Ser Pro Lys Pro Leu Leu Asp Gly Arg Glu 
    370                 375                 380                 


Val Met Arg Leu Leu Gly Leu Glu Pro Gly Pro Lys Val Gly Glu Ala 
385                 390                 395                 400 


Leu Ala Ala Leu Leu Glu Ala Gln Ala Ile Gly Glu Val Gln Ser Arg 
                405                 410                 415     


Thr Glu Ala Ala Ser Phe Leu Arg Asp Ala Phe Gly Glu Asp Lys Gly 
            420                 425                 430         


Arg Met Ser Gly Gly Gly Gly Gly Gly Cys 
        435                 440         


<210>  23
<211>  1955
<212>  RNA
<213>  Artificial sequence

<220>
<223>  hemagglutinin influenza A virus 5'-TOP-UTR GC-optimized 
       polyA_signal poly(C)_signal

<400>  23
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagg       60

ccauccuggu gguccuccug uacaccuucg ccaccgcgaa cgccgacacg cugugcaucg      120

gcuaccacgc caacaacagc accgacaccg uggacaccgu gcucgagaag aacgucacgg      180

ugacccacuc cgugaaccug cuggaggaca agcacaacgg gaagcucugc aagcugcggg      240

gcgucgcccc gcugcaccuc gggaagugca acaucgccgg cuggauccug gggaacccgg      300

agugcgagag ccuguccacc gcgagcuccu ggagcuacau cguggagacc uccagcuccg      360

acaacggcac gugcuacccc ggcgacuuca ucgacuacga ggagcuccgc gagcagcuga      420

gcuccgugag cuccuucgag cgguucgaga ucuuccccaa gaccagcucc uggcccaacc      480

acgacagcaa caaggggguc accgccgccu gcccgcacgc cggcgcgaag uccuucuaca      540

agaaccugau cuggcucgug aagaagggga acagcuaccc caagcugucc aagagcuaca      600

ucaacgacaa gggcaaggag gugcuggucc ucugggggau ccaccacccc agcaccuccg      660

ccgaccagca gagccuguac cagaacgccg acgccuacgu guucgugggc uccagccgcu      720

acuccaagaa guucaagccc gagaucgcca uccggccgaa gguccgcgac caggagggcc      780

ggaugaacua cuacuggacg cugguggagc ccggggacaa gaucaccuuc gaggcgaccg      840

gcaaccucgu ggucccccgc uacgccuucg ccauggagcg gaacgccggg agcggcauca      900

ucaucuccga cacccccgug cacgacugca acacgaccug ccagaccccg aagggcgcca      960

ucaacaccag ccugcccuuc cagaacaucc accccaucac gaucgggaag ugccccaagu     1020

acgugaaguc caccaagcug cgccucgcga ccggccugcg gaacgucccg agcauccagu     1080

cccgcgggcu guucggcgcc aucgccgggu ucaucgaggg cggcuggacc gggauggugg     1140

acggcuggua cggguaccac caccagaacg agcagggcag cggguacgcc gccgaccuca     1200

aguccacgca gaacgcgauc gacgagauca ccaacaaggu gaacagcguc aucgagaaga     1260

ugaacaccca guucaccgcc gugggcaagg aguucaacca ccuggagaag cggaucgaga     1320

accugaacaa gaaggucgac gacggcuucc ucgacaucug gacguacaac gccgagcugc     1380

uggugcuccu ggagaacgag cgcacccugg acuaccacga cuccaacgug aagaaccucu     1440

acgagaaggu ccggagccag cugaagaaca acgccaagga gaucgggaac ggcugcuucg     1500

aguucuacca caagugcgac aacaccugca uggaguccgu gaagaacggg accuacgacu     1560

accccaagua cagcgaggag gccaagcuga accgcgagga gaucgacggc gugaagcucg     1620

aguccacgcg gaucuaccag auccuggcga ucuacagcac cgucgccagc ucccuggugc     1680

ucguggucag ccugggggcc aucuccuucu ggaugugcag caacggcucc cugcagugcc     1740

gcaucugcau cugaccacua gugcaucaca uuuaaaagca ucucagccua ccaugagaau     1800

aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc guugguguaa     1860

agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu uuucucugug     1920

cuucaauuaa uaaaaaaugg aaagaaccua gaucu                                1955


<210>  24
<211>  454
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256S

<400>  24

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Cys Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala 
    450                 


<210>  25
<211>  454
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256S

<400>  25

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Cys Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala 
    450                 


<210>  26
<211>  454
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256A

<400>  26

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Cys Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala 
    450                 


<210>  27
<211>  454
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256V

<400>  27

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Cys Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala 
    450                 


<210>  28
<211>  454
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256V

<400>  28

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Cys Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala 
    450                 


<210>  29
<211>  454
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256V

<400>  29

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Cys Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala 
    450                 


<210>  30
<211>  454
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256A

<400>  30

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Cys Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala 
    450                 


<210>  31
<211>  454
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256S

<400>  31

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Cys Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala 
    450                 


<210>  32
<211>  455
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256S C346S 455C

<400>  32

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Cys 
    450                 455 


<210>  33
<211>  455
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256S C346S 455C

<400>  33

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Cys 
    450                 455 


<210>  34
<211>  455
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256A C346S 455C

<400>  34

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Cys 
    450                 455 


<210>  35
<211>  455
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256S C346A 455C

<400>  35

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Cys 
    450                 455 


<210>  36
<211>  455
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256A C346S 455C

<400>  36

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Cys 
    450                 455 


<210>  37
<211>  455
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256A C346A 455C

<400>  37

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Cys 
    450                 455 


<210>  38
<211>  455
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256S C346A 455C

<400>  38

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Cys 
    450                 455 


<210>  39
<211>  455
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256V C346V 455C

<400>  39

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Cys 
    450                 455 


<210>  40
<211>  455
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256V C346V 455C

<400>  40

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Cys 
    450                 455 


<210>  41
<211>  455
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256A C346V 455C

<400>  41

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Cys 
    450                 455 


<210>  42
<211>  455
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256V C346A 455C

<400>  42

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Cys 
    450                 455 


<210>  43
<211>  455
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256A C346V 455C

<400>  43

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Cys 
    450                 455 


<210>  44
<211>  455
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256A C346A 455C

<400>  44

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Cys 
    450                 455 


<210>  45
<211>  455
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256V C346A 455C

<400>  45

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Cys 
    450                 455 


<210>  46
<211>  455
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256S C346V 455C

<400>  46

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Cys 
    450                 455 


<210>  47
<211>  455
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256A C346V 455C

<400>  47

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Cys 
    450                 455 


<210>  48
<211>  455
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256S C346V 455C

<400>  48

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Cys 
    450                 455 


<210>  49
<211>  455
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256V C346S 455C

<400>  49

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Cys 
    450                 455 


<210>  50
<211>  455
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256V C346A 455C

<400>  50

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Cys 
    450                 455 


<210>  51
<211>  455
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256V C346S 455C

<400>  51

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Cys 
    450                 455 


<210>  52
<211>  455
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256S C346S 455C

<400>  52

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Cys 
    450                 455 


<210>  53
<211>  455
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256S C346A 455C

<400>  53

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Cys 
    450                 455 


<210>  54
<211>  455
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256A C346S 455C

<400>  54

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Cys 
    450                 455 


<210>  55
<211>  455
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256V C346S 455C

<400>  55

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Cys 
    450                 455 


<210>  56
<211>  455
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256S C346V 455C

<400>  56

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Cys 
    450                 455 


<210>  57
<211>  455
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256V C346V 455C

<400>  57

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Cys 
    450                 455 


<210>  58
<211>  461
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256S C346S 455 (6xG)C

<400>  58

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Gly Gly Cys 
    450                 455                 460     


<210>  59
<211>  461
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256S C346S 455 (6xG)C

<400>  59

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Gly Gly Cys 
    450                 455                 460     


<210>  60
<211>  461
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256A C346S 455 (6xG)C

<400>  60

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Gly Gly Cys 
    450                 455                 460     


<210>  61
<211>  461
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256S C346A 455 (6xG)C

<400>  61

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Gly Gly Cys 
    450                 455                 460     


<210>  62
<211>  461
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256A C346S 455 (6xG)C

<400>  62

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Gly Gly Cys 
    450                 455                 460     


<210>  63
<211>  461
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256A C346A 455 (6xG)C

<400>  63

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Gly Gly Cys 
    450                 455                 460     


<210>  64
<211>  461
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256S C346A 455 (6xG)C

<400>  64

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Gly Gly Cys 
    450                 455                 460     


<210>  65
<211>  461
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256V C346V 455 (6xG)C

<400>  65

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Gly Gly Cys 
    450                 455                 460     


<210>  66
<211>  461
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256V C346V 455 (6xG)C

<400>  66

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Gly Gly Cys 
    450                 455                 460     


<210>  67
<211>  461
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256A C346V 455 (6xG)C

<400>  67

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Gly Gly Cys 
    450                 455                 460     


<210>  68
<211>  461
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256V C346A 455 (6xG)C

<400>  68

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Gly Gly Cys 
    450                 455                 460     


<210>  69
<211>  461
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256A C346V 455 (6xG)C

<400>  69

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Gly Gly Cys 
    450                 455                 460     


<210>  70
<211>  461
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256A C346A 455 (6xG)C

<400>  70

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Gly Gly Cys 
    450                 455                 460     


<210>  71
<211>  461
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256V C346A 455 (6xG)C

<400>  71

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Gly Gly Cys 
    450                 455                 460     


<210>  72
<211>  461
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256S C346V 455 (6xG)C

<400>  72

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Gly Gly Cys 
    450                 455                 460     


<210>  73
<211>  461
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256A C346V 455 (6xG)C

<400>  73

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Gly Gly Cys 
    450                 455                 460     


<210>  74
<211>  461
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256S C346V 455 (6xG)C

<400>  74

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Gly Gly Cys 
    450                 455                 460     


<210>  75
<211>  461
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256V C346S 455 (6xG)C

<400>  75

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Gly Gly Cys 
    450                 455                 460     


<210>  76
<211>  461
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256V C346A 455 (6xG)C

<400>  76

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Gly Gly Cys 
    450                 455                 460     


<210>  77
<211>  461
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256V C346S 455 (6xG)C

<400>  77

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Gly Gly Cys 
    450                 455                 460     


<210>  78
<211>  461
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256S C346S 455 (6xG)C

<400>  78

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Gly Gly Cys 
    450                 455                 460     


<210>  79
<211>  461
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256S C346A 455 (6xG)C

<400>  79

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Gly Gly Cys 
    450                 455                 460     


<210>  80
<211>  461
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256A C346S 455 (6xG)C

<400>  80

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Gly Gly Cys 
    450                 455                 460     


<210>  81
<211>  461
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256V C346S 455 (6xG)C

<400>  81

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Gly Gly Cys 
    450                 455                 460     


<210>  82
<211>  461
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256S C346V 455 (6xG)C

<400>  82

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Gly Gly Cys 
    450                 455                 460     


<210>  83
<211>  461
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256V C346V  455 (6xG)C

<400>  83

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Gly Gly Cys 
    450                 455                 460     


<210>  84
<211>  461
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB (6xG)C

<400>  84

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Cys Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Cys 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Cys Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Gly Gly Cys 
    450                 455                 460     


<210>  85
<211>  465
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256A C346A 455 (flexLinker)C

<400>  85

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


Cys 
465 


<210>  86
<211>  465
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256S C346S 455 (flexLinker)C

<400>  86

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


Cys 
465 


<210>  87
<211>  465
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256S C346S 455 (flexLinker)C

<400>  87

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


Cys 
465 


<210>  88
<211>  465
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256A C346S 455(flexLinker)C

<400>  88

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


Cys 
465 


<210>  89
<211>  465
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256S C346A 455 (flexLinker)C

<400>  89

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


Cys 
465 


<210>  90
<211>  465
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256A C346S 455 (flexLinker)C

<400>  90

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


Cys 
465 


<210>  91
<211>  465
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256A C346A 455 (flexLinker)C

<400>  91

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


Cys 
465 


<210>  92
<211>  465
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256S C346A 455 (flexLinker)C

<400>  92

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


Cys 
465 


<210>  93
<211>  465
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256V C346V 455 (flexLinker)C

<400>  93

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


Cys 
465 


<210>  94
<211>  465
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256V C346V 455 (flexLinker)C

<400>  94

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


Cys 
465 


<210>  95
<211>  465
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256A C346V 455 (flexLinker)C

<400>  95

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


Cys 
465 


<210>  96
<211>  465
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256V C346A 455 (flexLinker)C

<400>  96

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


Cys 
465 


<210>  97
<211>  465
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256A C346V 455 (flexLinker)C

<400>  97

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


Cys 
465 


<210>  98
<211>  465
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256A C346A 455 (flexLinker)C

<400>  98

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


Cys 
465 


<210>  99
<211>  465
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256V C346A 455 (flexLinker)C

<400>  99

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


Cys 
465 


<210>  100
<211>  465
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256S C346V 455 (flexLinker)C

<400>  100

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


Cys 
465 


<210>  101
<211>  465
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256A C346V 455 (flexLinker)C

<400>  101

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


Cys 
465 


<210>  102
<211>  465
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256S C346V 455 (flexLinker)C

<400>  102

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


Cys 
465 


<210>  103
<211>  465
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256V C346S 455 (flexLinker)C

<400>  103

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


Cys 
465 


<210>  104
<211>  465
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256V C346A 455 (flexLinker)C

<400>  104

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


Cys 
465 


<210>  105
<211>  465
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256V C346S 455 (flexLinker)C

<400>  105

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


Cys 
465 


<210>  106
<211>  465
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256S C346S 455 (flexLinker)C

<400>  106

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


Cys 
465 


<210>  107
<211>  465
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256S C346A 455 (flexLinker)C

<400>  107

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


Cys 
465 


<210>  108
<211>  465
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256A C346S 455 (flexLinker)C

<400>  108

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


Cys 
465 


<210>  109
<211>  465
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256V C346S 455 (flexLinker)C

<400>  109

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


Cys 
465 


<210>  110
<211>  465
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256S C346V 455 (flexLinker)C

<400>  110

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


Cys 
465 


<210>  111
<211>  465
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256V C346V 455 (flexLinker)C

<400>  111

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


Cys 
465 


<210>  112
<211>  465
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB (flexLinker)C

<400>  112

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Cys Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Cys 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Cys Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


Cys 
465 


<210>  113
<211>  471
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256A C346A 455 (flexLinker+HIS)C

<400>  113

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


His His His His His His Cys 
465                 470     


<210>  114
<211>  471
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256S C346S 455 (flexLinker+HIS)C

<400>  114

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


His His His His His His Cys 
465                 470     


<210>  115
<211>  471
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256S C346S 455 (flexLinker+HIS)C

<400>  115

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


His His His His His His Cys 
465                 470     


<210>  116
<211>  471
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256A C346S 455 (flexLinker+HIS)C

<400>  116

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


His His His His His His Cys 
465                 470     


<210>  117
<211>  471
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256S C346A 455 (flexLinker+HIS)C

<400>  117

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


His His His His His His Cys 
465                 470     


<210>  118
<211>  471
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256A C346S 455 (flexLinker+HIS)C

<400>  118

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


His His His His His His Cys 
465                 470     


<210>  119
<211>  471
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256A C346A 455 (flexLinker+HIS)C

<400>  119

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


His His His His His His Cys 
465                 470     


<210>  120
<211>  471
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256S C346A 455 (flexLinker+HIS)C

<400>  120

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


His His His His His His Cys 
465                 470     


<210>  121
<211>  471
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256V C346V 455 (flexLinker+HIS)C

<400>  121

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


His His His His His His Cys 
465                 470     


<210>  122
<211>  471
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256V C346V 455 (flexLinker+HIS)C

<400>  122

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


His His His His His His Cys 
465                 470     


<210>  123
<211>  471
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256A C346V 455 (flexLinker+HIS)C

<400>  123

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


His His His His His His Cys 
465                 470     


<210>  124
<211>  471
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256V C346A 455 (flexLinker+HIS)C

<400>  124

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


His His His His His His Cys 
465                 470     


<210>  125
<211>  471
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256A C346V 455 (flexLinker+HIS)C

<400>  125

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


His His His His His His Cys 
465                 470     


<210>  126
<211>  471
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256A C346A 455 (flexLinker+HIS)C

<400>  126

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


His His His His His His Cys 
465                 470     


<210>  127
<211>  471
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256V C346A 455 (flexLinker+HIS)C

<400>  127

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


His His His His His His Cys 
465                 470     


<210>  128
<211>  471
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256S C346V 455 (flexLinker+HIS)C

<400>  128

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


His His His His His His Cys 
465                 470     


<210>  129
<211>  471
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256A C346V 455 (flexLinker+HIS)C

<400>  129

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


His His His His His His Cys 
465                 470     


<210>  130
<211>  471
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256S C346V 455 (flexLinker+HIS)C

<400>  130

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


His His His His His His Cys 
465                 470     


<210>  131
<211>  471
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256V C346S 455 (flexLinker+HIS)C

<400>  131

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


His His His His His His Cys 
465                 470     


<210>  132
<211>  471
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256V C346A 455 (flexLinker+HIS)C

<400>  132

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


His His His His His His Cys 
465                 470     


<210>  133
<211>  471
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88A C256V C346S 455 (flexLinker+HIS)C

<400>  133

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ala Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


His His His His His His Cys 
465                 470     


<210>  134
<211>  471
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256S C346S 455 (flexLinker+HIS)C

<400>  134

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


His His His His His His Cys 
465                 470     


<210>  135
<211>  471
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256S C346A 455 (flexLinker+HIS)C

<400>  135

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


His His His His His His Cys 
465                 470     


<210>  136
<211>  471
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256A C346S 455 (flexLinker+HIS)C

<400>  136

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ala 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


His His His His His His Cys 
465                 470     


<210>  137
<211>  471
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256V C346S 455 (flexLinker+HIS)C

<400>  137

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ser Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


His His His His His His Cys 
465                 470     


<210>  138
<211>  471
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88V C256S C346V 455 (flexLinker+HIS)C

<400>  138

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Val Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Ser 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


His His His His His His Cys 
465                 470     


<210>  139
<211>  471
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256V C346V 455 (flexLinker+HIS)C

<400>  139

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Val 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Val Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


His His His His His His Cys 
465                 470     


<210>  140
<211>  471
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB  (flexLinker+HIS)C

<400>  140

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Cys Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Cys 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Cys Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


His His His His His His Cys 
465                 470     


<210>  141
<211>  439
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Thermus aquaticus poly(A) polymerase C190S 439C

<400>  141

Met Leu Arg Leu Ile Ala His Arg Asp Phe Pro Phe Tyr Thr Pro Lys 
1               5                   10                  15      


Gly Ala Ile Pro Val Gly Gly Ala Leu Arg Asp Leu Leu Leu Gly Glu 
            20                  25                  30          


Arg Pro Leu Asp Leu Asp Phe Ala Ala Trp Asp Pro Glu Lys Ala Ala 
        35                  40                  45              


Glu Glu Ala Lys Gly Arg Tyr Gly Gly Thr Leu Phe Pro Leu Asp Pro 
    50                  55                  60                  


Glu Arg Gly His Tyr Arg Leu Val Ala Gly Ala Leu Thr Leu Asp Phe 
65                  70                  75                  80  


Thr Pro Leu Glu Gly Ser Pro Glu Glu Asp Leu Leu Arg Arg Asp Tyr 
                85                  90                  95      


Arg Val Asn Ala Leu Leu Trp Leu Lys Gly Arg Val Phe Gly Leu Pro 
            100                 105                 110         


Gly Ala Glu Glu Asp Leu Asn Arg Arg Leu Leu Val Pro Val Gln Glu 
        115                 120                 125             


Glu Asn Leu Tyr Ala Asp Pro Leu Arg Ser Leu Arg Gly Val Arg Leu 
    130                 135                 140                 


Ala Ala Thr Leu Gly Leu Gly Leu Pro Pro Glu Thr Arg Arg Ala Leu 
145                 150                 155                 160 


Ala Arg His Ala Arg Phe Leu Gln Glu Asn Pro Ser Ala Leu Pro Ala 
                165                 170                 175     


Arg Glu Arg Val Arg Glu Glu Leu Ala Lys Leu Leu Leu Ser Lys Arg 
            180                 185                 190         


Ala Pro Phe Gly Leu His Leu Leu Glu Arg Thr Gly Leu Leu Gly Ile 
        195                 200                 205             


Tyr Leu Pro Glu Leu Thr His Leu Val Gly Leu His Gln Gly Gly Val 
    210                 215                 220                 


His His Leu Pro Ala Trp Glu His Thr Leu Ser Val Leu Phe His Leu 
225                 230                 235                 240 


Leu Trp Leu Trp Pro Glu Ala Pro Leu Glu Ala Arg Leu Ala Ala Leu 
                245                 250                 255     


Tyr His Asp Ala Gly Lys Pro Leu Thr Arg Arg Tyr Asp Pro Glu Val 
            260                 265                 270         


Gly Arg Tyr Arg Phe Phe Gly His Ala Glu Val Gly Ala Glu Met Ala 
        275                 280                 285             


Lys Ala Ala Leu Phe Trp Leu Arg Phe Pro Lys Glu Val Val Glu Lys 
    290                 295                 300                 


Ala Ala His Leu Val Arg Arg His Met Asp Arg Pro Pro Glu Gly Thr 
305                 310                 315                 320 


Lys Ala Leu Arg Arg Phe Tyr Phe Lys Arg Arg Asp Leu Leu Pro Ala 
                325                 330                 335     


Leu Pro Tyr Leu Met Ala Ala Asp Arg Leu Ala Ala Lys Gly Val Glu 
            340                 345                 350         


Ala Glu Ala Trp Pro Leu Leu Glu Ala Tyr Arg Glu Ala Leu Lys Thr 
        355                 360                 365             


Pro Leu Pro Gln Glu Pro Leu Leu Ser Gly Glu Glu Val Met Ala Leu 
    370                 375                 380                 


Leu Gly Leu Arg Pro Gly Pro Leu Val Gly Glu Ala Leu Arg Ala Leu 
385                 390                 395                 400 


Leu Glu Ala Gln Ala Glu Gly Ser Val Ala Ser Gln Glu Glu Ala Arg 
                405                 410                 415     


Arg Phe Leu Leu Tyr Trp Arg Asp Gly Arg Thr Ala Gln Ala Thr Pro 
            420                 425                 430         


Ala Pro Asp His Pro Gly Cys 
        435                 


<210>  142
<211>  439
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Thermus aquaticus poly(A) polymerase C190V 439C

<400>  142

Met Leu Arg Leu Ile Ala His Arg Asp Phe Pro Phe Tyr Thr Pro Lys 
1               5                   10                  15      


Gly Ala Ile Pro Val Gly Gly Ala Leu Arg Asp Leu Leu Leu Gly Glu 
            20                  25                  30          


Arg Pro Leu Asp Leu Asp Phe Ala Ala Trp Asp Pro Glu Lys Ala Ala 
        35                  40                  45              


Glu Glu Ala Lys Gly Arg Tyr Gly Gly Thr Leu Phe Pro Leu Asp Pro 
    50                  55                  60                  


Glu Arg Gly His Tyr Arg Leu Val Ala Gly Ala Leu Thr Leu Asp Phe 
65                  70                  75                  80  


Thr Pro Leu Glu Gly Ser Pro Glu Glu Asp Leu Leu Arg Arg Asp Tyr 
                85                  90                  95      


Arg Val Asn Ala Leu Leu Trp Leu Lys Gly Arg Val Phe Gly Leu Pro 
            100                 105                 110         


Gly Ala Glu Glu Asp Leu Asn Arg Arg Leu Leu Val Pro Val Gln Glu 
        115                 120                 125             


Glu Asn Leu Tyr Ala Asp Pro Leu Arg Ser Leu Arg Gly Val Arg Leu 
    130                 135                 140                 


Ala Ala Thr Leu Gly Leu Gly Leu Pro Pro Glu Thr Arg Arg Ala Leu 
145                 150                 155                 160 


Ala Arg His Ala Arg Phe Leu Gln Glu Asn Pro Ser Ala Leu Pro Ala 
                165                 170                 175     


Arg Glu Arg Val Arg Glu Glu Leu Ala Lys Leu Leu Leu Val Lys Arg 
            180                 185                 190         


Ala Pro Phe Gly Leu His Leu Leu Glu Arg Thr Gly Leu Leu Gly Ile 
        195                 200                 205             


Tyr Leu Pro Glu Leu Thr His Leu Val Gly Leu His Gln Gly Gly Val 
    210                 215                 220                 


His His Leu Pro Ala Trp Glu His Thr Leu Ser Val Leu Phe His Leu 
225                 230                 235                 240 


Leu Trp Leu Trp Pro Glu Ala Pro Leu Glu Ala Arg Leu Ala Ala Leu 
                245                 250                 255     


Tyr His Asp Ala Gly Lys Pro Leu Thr Arg Arg Tyr Asp Pro Glu Val 
            260                 265                 270         


Gly Arg Tyr Arg Phe Phe Gly His Ala Glu Val Gly Ala Glu Met Ala 
        275                 280                 285             


Lys Ala Ala Leu Phe Trp Leu Arg Phe Pro Lys Glu Val Val Glu Lys 
    290                 295                 300                 


Ala Ala His Leu Val Arg Arg His Met Asp Arg Pro Pro Glu Gly Thr 
305                 310                 315                 320 


Lys Ala Leu Arg Arg Phe Tyr Phe Lys Arg Arg Asp Leu Leu Pro Ala 
                325                 330                 335     


Leu Pro Tyr Leu Met Ala Ala Asp Arg Leu Ala Ala Lys Gly Val Glu 
            340                 345                 350         


Ala Glu Ala Trp Pro Leu Leu Glu Ala Tyr Arg Glu Ala Leu Lys Thr 
        355                 360                 365             


Pro Leu Pro Gln Glu Pro Leu Leu Ser Gly Glu Glu Val Met Ala Leu 
    370                 375                 380                 


Leu Gly Leu Arg Pro Gly Pro Leu Val Gly Glu Ala Leu Arg Ala Leu 
385                 390                 395                 400 


Leu Glu Ala Gln Ala Glu Gly Ser Val Ala Ser Gln Glu Glu Ala Arg 
                405                 410                 415     


Arg Phe Leu Leu Tyr Trp Arg Asp Gly Arg Thr Ala Gln Ala Thr Pro 
            420                 425                 430         


Ala Pro Asp His Pro Gly Cys 
        435                 


<210>  143
<211>  445
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Thermus aquaticus poly(A) polymerase C190S 439(6xG)C

<400>  143

Met Leu Arg Leu Ile Ala His Arg Asp Phe Pro Phe Tyr Thr Pro Lys 
1               5                   10                  15      


Gly Ala Ile Pro Val Gly Gly Ala Leu Arg Asp Leu Leu Leu Gly Glu 
            20                  25                  30          


Arg Pro Leu Asp Leu Asp Phe Ala Ala Trp Asp Pro Glu Lys Ala Ala 
        35                  40                  45              


Glu Glu Ala Lys Gly Arg Tyr Gly Gly Thr Leu Phe Pro Leu Asp Pro 
    50                  55                  60                  


Glu Arg Gly His Tyr Arg Leu Val Ala Gly Ala Leu Thr Leu Asp Phe 
65                  70                  75                  80  


Thr Pro Leu Glu Gly Ser Pro Glu Glu Asp Leu Leu Arg Arg Asp Tyr 
                85                  90                  95      


Arg Val Asn Ala Leu Leu Trp Leu Lys Gly Arg Val Phe Gly Leu Pro 
            100                 105                 110         


Gly Ala Glu Glu Asp Leu Asn Arg Arg Leu Leu Val Pro Val Gln Glu 
        115                 120                 125             


Glu Asn Leu Tyr Ala Asp Pro Leu Arg Ser Leu Arg Gly Val Arg Leu 
    130                 135                 140                 


Ala Ala Thr Leu Gly Leu Gly Leu Pro Pro Glu Thr Arg Arg Ala Leu 
145                 150                 155                 160 


Ala Arg His Ala Arg Phe Leu Gln Glu Asn Pro Ser Ala Leu Pro Ala 
                165                 170                 175     


Arg Glu Arg Val Arg Glu Glu Leu Ala Lys Leu Leu Leu Ser Lys Arg 
            180                 185                 190         


Ala Pro Phe Gly Leu His Leu Leu Glu Arg Thr Gly Leu Leu Gly Ile 
        195                 200                 205             


Tyr Leu Pro Glu Leu Thr His Leu Val Gly Leu His Gln Gly Gly Val 
    210                 215                 220                 


His His Leu Pro Ala Trp Glu His Thr Leu Ser Val Leu Phe His Leu 
225                 230                 235                 240 


Leu Trp Leu Trp Pro Glu Ala Pro Leu Glu Ala Arg Leu Ala Ala Leu 
                245                 250                 255     


Tyr His Asp Ala Gly Lys Pro Leu Thr Arg Arg Tyr Asp Pro Glu Val 
            260                 265                 270         


Gly Arg Tyr Arg Phe Phe Gly His Ala Glu Val Gly Ala Glu Met Ala 
        275                 280                 285             


Lys Ala Ala Leu Phe Trp Leu Arg Phe Pro Lys Glu Val Val Glu Lys 
    290                 295                 300                 


Ala Ala His Leu Val Arg Arg His Met Asp Arg Pro Pro Glu Gly Thr 
305                 310                 315                 320 


Lys Ala Leu Arg Arg Phe Tyr Phe Lys Arg Arg Asp Leu Leu Pro Ala 
                325                 330                 335     


Leu Pro Tyr Leu Met Ala Ala Asp Arg Leu Ala Ala Lys Gly Val Glu 
            340                 345                 350         


Ala Glu Ala Trp Pro Leu Leu Glu Ala Tyr Arg Glu Ala Leu Lys Thr 
        355                 360                 365             


Pro Leu Pro Gln Glu Pro Leu Leu Ser Gly Glu Glu Val Met Ala Leu 
    370                 375                 380                 


Leu Gly Leu Arg Pro Gly Pro Leu Val Gly Glu Ala Leu Arg Ala Leu 
385                 390                 395                 400 


Leu Glu Ala Gln Ala Glu Gly Ser Val Ala Ser Gln Glu Glu Ala Arg 
                405                 410                 415     


Arg Phe Leu Leu Tyr Trp Arg Asp Gly Arg Thr Ala Gln Ala Thr Pro 
            420                 425                 430         


Ala Pro Asp His Pro Gly Gly Gly Gly Gly Gly Gly Cys 
        435                 440                 445 


<210>  144
<211>  445
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Thermus aquaticus poly(A) polymerase C190V 439(6xG)C

<400>  144

Met Leu Arg Leu Ile Ala His Arg Asp Phe Pro Phe Tyr Thr Pro Lys 
1               5                   10                  15      


Gly Ala Ile Pro Val Gly Gly Ala Leu Arg Asp Leu Leu Leu Gly Glu 
            20                  25                  30          


Arg Pro Leu Asp Leu Asp Phe Ala Ala Trp Asp Pro Glu Lys Ala Ala 
        35                  40                  45              


Glu Glu Ala Lys Gly Arg Tyr Gly Gly Thr Leu Phe Pro Leu Asp Pro 
    50                  55                  60                  


Glu Arg Gly His Tyr Arg Leu Val Ala Gly Ala Leu Thr Leu Asp Phe 
65                  70                  75                  80  


Thr Pro Leu Glu Gly Ser Pro Glu Glu Asp Leu Leu Arg Arg Asp Tyr 
                85                  90                  95      


Arg Val Asn Ala Leu Leu Trp Leu Lys Gly Arg Val Phe Gly Leu Pro 
            100                 105                 110         


Gly Ala Glu Glu Asp Leu Asn Arg Arg Leu Leu Val Pro Val Gln Glu 
        115                 120                 125             


Glu Asn Leu Tyr Ala Asp Pro Leu Arg Ser Leu Arg Gly Val Arg Leu 
    130                 135                 140                 


Ala Ala Thr Leu Gly Leu Gly Leu Pro Pro Glu Thr Arg Arg Ala Leu 
145                 150                 155                 160 


Ala Arg His Ala Arg Phe Leu Gln Glu Asn Pro Ser Ala Leu Pro Ala 
                165                 170                 175     


Arg Glu Arg Val Arg Glu Glu Leu Ala Lys Leu Leu Leu Val Lys Arg 
            180                 185                 190         


Ala Pro Phe Gly Leu His Leu Leu Glu Arg Thr Gly Leu Leu Gly Ile 
        195                 200                 205             


Tyr Leu Pro Glu Leu Thr His Leu Val Gly Leu His Gln Gly Gly Val 
    210                 215                 220                 


His His Leu Pro Ala Trp Glu His Thr Leu Ser Val Leu Phe His Leu 
225                 230                 235                 240 


Leu Trp Leu Trp Pro Glu Ala Pro Leu Glu Ala Arg Leu Ala Ala Leu 
                245                 250                 255     


Tyr His Asp Ala Gly Lys Pro Leu Thr Arg Arg Tyr Asp Pro Glu Val 
            260                 265                 270         


Gly Arg Tyr Arg Phe Phe Gly His Ala Glu Val Gly Ala Glu Met Ala 
        275                 280                 285             


Lys Ala Ala Leu Phe Trp Leu Arg Phe Pro Lys Glu Val Val Glu Lys 
    290                 295                 300                 


Ala Ala His Leu Val Arg Arg His Met Asp Arg Pro Pro Glu Gly Thr 
305                 310                 315                 320 


Lys Ala Leu Arg Arg Phe Tyr Phe Lys Arg Arg Asp Leu Leu Pro Ala 
                325                 330                 335     


Leu Pro Tyr Leu Met Ala Ala Asp Arg Leu Ala Ala Lys Gly Val Glu 
            340                 345                 350         


Ala Glu Ala Trp Pro Leu Leu Glu Ala Tyr Arg Glu Ala Leu Lys Thr 
        355                 360                 365             


Pro Leu Pro Gln Glu Pro Leu Leu Ser Gly Glu Glu Val Met Ala Leu 
    370                 375                 380                 


Leu Gly Leu Arg Pro Gly Pro Leu Val Gly Glu Ala Leu Arg Ala Leu 
385                 390                 395                 400 


Leu Glu Ala Gln Ala Glu Gly Ser Val Ala Ser Gln Glu Glu Ala Arg 
                405                 410                 415     


Arg Phe Leu Leu Tyr Trp Arg Asp Gly Arg Thr Ala Gln Ala Thr Pro 
            420                 425                 430         


Ala Pro Asp His Pro Gly Gly Gly Gly Gly Gly Gly Cys 
        435                 440                 445 


<210>  145
<211>  445
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Thermus aquaticus poly(A) polymerase 439(6xG)C

<400>  145

Met Leu Arg Leu Ile Ala His Arg Asp Phe Pro Phe Tyr Thr Pro Lys 
1               5                   10                  15      


Gly Ala Ile Pro Val Gly Gly Ala Leu Arg Asp Leu Leu Leu Gly Glu 
            20                  25                  30          


Arg Pro Leu Asp Leu Asp Phe Ala Ala Trp Asp Pro Glu Lys Ala Ala 
        35                  40                  45              


Glu Glu Ala Lys Gly Arg Tyr Gly Gly Thr Leu Phe Pro Leu Asp Pro 
    50                  55                  60                  


Glu Arg Gly His Tyr Arg Leu Val Ala Gly Ala Leu Thr Leu Asp Phe 
65                  70                  75                  80  


Thr Pro Leu Glu Gly Ser Pro Glu Glu Asp Leu Leu Arg Arg Asp Tyr 
                85                  90                  95      


Arg Val Asn Ala Leu Leu Trp Leu Lys Gly Arg Val Phe Gly Leu Pro 
            100                 105                 110         


Gly Ala Glu Glu Asp Leu Asn Arg Arg Leu Leu Val Pro Val Gln Glu 
        115                 120                 125             


Glu Asn Leu Tyr Ala Asp Pro Leu Arg Ser Leu Arg Gly Val Arg Leu 
    130                 135                 140                 


Ala Ala Thr Leu Gly Leu Gly Leu Pro Pro Glu Thr Arg Arg Ala Leu 
145                 150                 155                 160 


Ala Arg His Ala Arg Phe Leu Gln Glu Asn Pro Ser Ala Leu Pro Ala 
                165                 170                 175     


Arg Glu Arg Val Arg Glu Glu Leu Ala Lys Leu Leu Leu Cys Lys Arg 
            180                 185                 190         


Ala Pro Phe Gly Leu His Leu Leu Glu Arg Thr Gly Leu Leu Gly Ile 
        195                 200                 205             


Tyr Leu Pro Glu Leu Thr His Leu Val Gly Leu His Gln Gly Gly Val 
    210                 215                 220                 


His His Leu Pro Ala Trp Glu His Thr Leu Ser Val Leu Phe His Leu 
225                 230                 235                 240 


Leu Trp Leu Trp Pro Glu Ala Pro Leu Glu Ala Arg Leu Ala Ala Leu 
                245                 250                 255     


Tyr His Asp Ala Gly Lys Pro Leu Thr Arg Arg Tyr Asp Pro Glu Val 
            260                 265                 270         


Gly Arg Tyr Arg Phe Phe Gly His Ala Glu Val Gly Ala Glu Met Ala 
        275                 280                 285             


Lys Ala Ala Leu Phe Trp Leu Arg Phe Pro Lys Glu Val Val Glu Lys 
    290                 295                 300                 


Ala Ala His Leu Val Arg Arg His Met Asp Arg Pro Pro Glu Gly Thr 
305                 310                 315                 320 


Lys Ala Leu Arg Arg Phe Tyr Phe Lys Arg Arg Asp Leu Leu Pro Ala 
                325                 330                 335     


Leu Pro Tyr Leu Met Ala Ala Asp Arg Leu Ala Ala Lys Gly Val Glu 
            340                 345                 350         


Ala Glu Ala Trp Pro Leu Leu Glu Ala Tyr Arg Glu Ala Leu Lys Thr 
        355                 360                 365             


Pro Leu Pro Gln Glu Pro Leu Leu Ser Gly Glu Glu Val Met Ala Leu 
    370                 375                 380                 


Leu Gly Leu Arg Pro Gly Pro Leu Val Gly Glu Ala Leu Arg Ala Leu 
385                 390                 395                 400 


Leu Glu Ala Gln Ala Glu Gly Ser Val Ala Ser Gln Glu Glu Ala Arg 
                405                 410                 415     


Arg Phe Leu Leu Tyr Trp Arg Asp Gly Arg Thr Ala Gln Ala Thr Pro 
            420                 425                 430         


Ala Pro Asp His Pro Gly Gly Gly Gly Gly Gly Gly Cys 
        435                 440                 445 


<210>  146
<211>  448
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Thermus aquaticus poly(A) polymerase C190A 439(flex linker)C

<400>  146

Met Leu Arg Leu Ile Ala His Arg Asp Phe Pro Phe Tyr Thr Pro Lys 
1               5                   10                  15      


Gly Ala Ile Pro Val Gly Gly Ala Leu Arg Asp Leu Leu Leu Gly Glu 
            20                  25                  30          


Arg Pro Leu Asp Leu Asp Phe Ala Ala Trp Asp Pro Glu Lys Ala Ala 
        35                  40                  45              


Glu Glu Ala Lys Gly Arg Tyr Gly Gly Thr Leu Phe Pro Leu Asp Pro 
    50                  55                  60                  


Glu Arg Gly His Tyr Arg Leu Val Ala Gly Ala Leu Thr Leu Asp Phe 
65                  70                  75                  80  


Thr Pro Leu Glu Gly Ser Pro Glu Glu Asp Leu Leu Arg Arg Asp Tyr 
                85                  90                  95      


Arg Val Asn Ala Leu Leu Trp Leu Lys Gly Arg Val Phe Gly Leu Pro 
            100                 105                 110         


Gly Ala Glu Glu Asp Leu Asn Arg Arg Leu Leu Val Pro Val Gln Glu 
        115                 120                 125             


Glu Asn Leu Tyr Ala Asp Pro Leu Arg Ser Leu Arg Gly Val Arg Leu 
    130                 135                 140                 


Ala Ala Thr Leu Gly Leu Gly Leu Pro Pro Glu Thr Arg Arg Ala Leu 
145                 150                 155                 160 


Ala Arg His Ala Arg Phe Leu Gln Glu Asn Pro Ser Ala Leu Pro Ala 
                165                 170                 175     


Arg Glu Arg Val Arg Glu Glu Leu Ala Lys Leu Leu Leu Ala Lys Arg 
            180                 185                 190         


Ala Pro Phe Gly Leu His Leu Leu Glu Arg Thr Gly Leu Leu Gly Ile 
        195                 200                 205             


Tyr Leu Pro Glu Leu Thr His Leu Val Gly Leu His Gln Gly Gly Val 
    210                 215                 220                 


His His Leu Pro Ala Trp Glu His Thr Leu Ser Val Leu Phe His Leu 
225                 230                 235                 240 


Leu Trp Leu Trp Pro Glu Ala Pro Leu Glu Ala Arg Leu Ala Ala Leu 
                245                 250                 255     


Tyr His Asp Ala Gly Lys Pro Leu Thr Arg Arg Tyr Asp Pro Glu Val 
            260                 265                 270         


Gly Arg Tyr Arg Phe Phe Gly His Ala Glu Val Gly Ala Glu Met Ala 
        275                 280                 285             


Lys Ala Ala Leu Phe Trp Leu Arg Phe Pro Lys Glu Val Val Glu Lys 
    290                 295                 300                 


Ala Ala His Leu Val Arg Arg His Met Asp Arg Pro Pro Glu Gly Thr 
305                 310                 315                 320 


Lys Ala Leu Arg Arg Phe Tyr Phe Lys Arg Arg Asp Leu Leu Pro Ala 
                325                 330                 335     


Leu Pro Tyr Leu Met Ala Ala Asp Arg Leu Ala Ala Lys Gly Val Glu 
            340                 345                 350         


Ala Glu Ala Trp Pro Leu Leu Glu Ala Tyr Arg Glu Ala Leu Lys Thr 
        355                 360                 365             


Pro Leu Pro Gln Glu Pro Leu Leu Ser Gly Glu Glu Val Met Ala Leu 
    370                 375                 380                 


Leu Gly Leu Arg Pro Gly Pro Leu Val Gly Glu Ala Leu Arg Ala Leu 
385                 390                 395                 400 


Leu Glu Ala Gln Ala Glu Gly Ser Val Ala Ser Gln Glu Glu Ala Arg 
                405                 410                 415     


Arg Phe Leu Leu Tyr Trp Arg Asp Gly Arg Thr Ala Gln Ala Thr Pro 
            420                 425                 430         


Ala Pro Asp His Pro Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Cys 
        435                 440                 445             


<210>  147
<211>  448
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Thermus aquaticus poly(A) polymerase C190S 439(flex linker)C

<400>  147

Met Leu Arg Leu Ile Ala His Arg Asp Phe Pro Phe Tyr Thr Pro Lys 
1               5                   10                  15      


Gly Ala Ile Pro Val Gly Gly Ala Leu Arg Asp Leu Leu Leu Gly Glu 
            20                  25                  30          


Arg Pro Leu Asp Leu Asp Phe Ala Ala Trp Asp Pro Glu Lys Ala Ala 
        35                  40                  45              


Glu Glu Ala Lys Gly Arg Tyr Gly Gly Thr Leu Phe Pro Leu Asp Pro 
    50                  55                  60                  


Glu Arg Gly His Tyr Arg Leu Val Ala Gly Ala Leu Thr Leu Asp Phe 
65                  70                  75                  80  


Thr Pro Leu Glu Gly Ser Pro Glu Glu Asp Leu Leu Arg Arg Asp Tyr 
                85                  90                  95      


Arg Val Asn Ala Leu Leu Trp Leu Lys Gly Arg Val Phe Gly Leu Pro 
            100                 105                 110         


Gly Ala Glu Glu Asp Leu Asn Arg Arg Leu Leu Val Pro Val Gln Glu 
        115                 120                 125             


Glu Asn Leu Tyr Ala Asp Pro Leu Arg Ser Leu Arg Gly Val Arg Leu 
    130                 135                 140                 


Ala Ala Thr Leu Gly Leu Gly Leu Pro Pro Glu Thr Arg Arg Ala Leu 
145                 150                 155                 160 


Ala Arg His Ala Arg Phe Leu Gln Glu Asn Pro Ser Ala Leu Pro Ala 
                165                 170                 175     


Arg Glu Arg Val Arg Glu Glu Leu Ala Lys Leu Leu Leu Ser Lys Arg 
            180                 185                 190         


Ala Pro Phe Gly Leu His Leu Leu Glu Arg Thr Gly Leu Leu Gly Ile 
        195                 200                 205             


Tyr Leu Pro Glu Leu Thr His Leu Val Gly Leu His Gln Gly Gly Val 
    210                 215                 220                 


His His Leu Pro Ala Trp Glu His Thr Leu Ser Val Leu Phe His Leu 
225                 230                 235                 240 


Leu Trp Leu Trp Pro Glu Ala Pro Leu Glu Ala Arg Leu Ala Ala Leu 
                245                 250                 255     


Tyr His Asp Ala Gly Lys Pro Leu Thr Arg Arg Tyr Asp Pro Glu Val 
            260                 265                 270         


Gly Arg Tyr Arg Phe Phe Gly His Ala Glu Val Gly Ala Glu Met Ala 
        275                 280                 285             


Lys Ala Ala Leu Phe Trp Leu Arg Phe Pro Lys Glu Val Val Glu Lys 
    290                 295                 300                 


Ala Ala His Leu Val Arg Arg His Met Asp Arg Pro Pro Glu Gly Thr 
305                 310                 315                 320 


Lys Ala Leu Arg Arg Phe Tyr Phe Lys Arg Arg Asp Leu Leu Pro Ala 
                325                 330                 335     


Leu Pro Tyr Leu Met Ala Ala Asp Arg Leu Ala Ala Lys Gly Val Glu 
            340                 345                 350         


Ala Glu Ala Trp Pro Leu Leu Glu Ala Tyr Arg Glu Ala Leu Lys Thr 
        355                 360                 365             


Pro Leu Pro Gln Glu Pro Leu Leu Ser Gly Glu Glu Val Met Ala Leu 
    370                 375                 380                 


Leu Gly Leu Arg Pro Gly Pro Leu Val Gly Glu Ala Leu Arg Ala Leu 
385                 390                 395                 400 


Leu Glu Ala Gln Ala Glu Gly Ser Val Ala Ser Gln Glu Glu Ala Arg 
                405                 410                 415     


Arg Phe Leu Leu Tyr Trp Arg Asp Gly Arg Thr Ala Gln Ala Thr Pro 
            420                 425                 430         


Ala Pro Asp His Pro Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Cys 
        435                 440                 445             


<210>  148
<211>  448
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Thermus aquaticus poly(A) polymerase C190V 439(flex linker)C

<400>  148

Met Leu Arg Leu Ile Ala His Arg Asp Phe Pro Phe Tyr Thr Pro Lys 
1               5                   10                  15      


Gly Ala Ile Pro Val Gly Gly Ala Leu Arg Asp Leu Leu Leu Gly Glu 
            20                  25                  30          


Arg Pro Leu Asp Leu Asp Phe Ala Ala Trp Asp Pro Glu Lys Ala Ala 
        35                  40                  45              


Glu Glu Ala Lys Gly Arg Tyr Gly Gly Thr Leu Phe Pro Leu Asp Pro 
    50                  55                  60                  


Glu Arg Gly His Tyr Arg Leu Val Ala Gly Ala Leu Thr Leu Asp Phe 
65                  70                  75                  80  


Thr Pro Leu Glu Gly Ser Pro Glu Glu Asp Leu Leu Arg Arg Asp Tyr 
                85                  90                  95      


Arg Val Asn Ala Leu Leu Trp Leu Lys Gly Arg Val Phe Gly Leu Pro 
            100                 105                 110         


Gly Ala Glu Glu Asp Leu Asn Arg Arg Leu Leu Val Pro Val Gln Glu 
        115                 120                 125             


Glu Asn Leu Tyr Ala Asp Pro Leu Arg Ser Leu Arg Gly Val Arg Leu 
    130                 135                 140                 


Ala Ala Thr Leu Gly Leu Gly Leu Pro Pro Glu Thr Arg Arg Ala Leu 
145                 150                 155                 160 


Ala Arg His Ala Arg Phe Leu Gln Glu Asn Pro Ser Ala Leu Pro Ala 
                165                 170                 175     


Arg Glu Arg Val Arg Glu Glu Leu Ala Lys Leu Leu Leu Val Lys Arg 
            180                 185                 190         


Ala Pro Phe Gly Leu His Leu Leu Glu Arg Thr Gly Leu Leu Gly Ile 
        195                 200                 205             


Tyr Leu Pro Glu Leu Thr His Leu Val Gly Leu His Gln Gly Gly Val 
    210                 215                 220                 


His His Leu Pro Ala Trp Glu His Thr Leu Ser Val Leu Phe His Leu 
225                 230                 235                 240 


Leu Trp Leu Trp Pro Glu Ala Pro Leu Glu Ala Arg Leu Ala Ala Leu 
                245                 250                 255     


Tyr His Asp Ala Gly Lys Pro Leu Thr Arg Arg Tyr Asp Pro Glu Val 
            260                 265                 270         


Gly Arg Tyr Arg Phe Phe Gly His Ala Glu Val Gly Ala Glu Met Ala 
        275                 280                 285             


Lys Ala Ala Leu Phe Trp Leu Arg Phe Pro Lys Glu Val Val Glu Lys 
    290                 295                 300                 


Ala Ala His Leu Val Arg Arg His Met Asp Arg Pro Pro Glu Gly Thr 
305                 310                 315                 320 


Lys Ala Leu Arg Arg Phe Tyr Phe Lys Arg Arg Asp Leu Leu Pro Ala 
                325                 330                 335     


Leu Pro Tyr Leu Met Ala Ala Asp Arg Leu Ala Ala Lys Gly Val Glu 
            340                 345                 350         


Ala Glu Ala Trp Pro Leu Leu Glu Ala Tyr Arg Glu Ala Leu Lys Thr 
        355                 360                 365             


Pro Leu Pro Gln Glu Pro Leu Leu Ser Gly Glu Glu Val Met Ala Leu 
    370                 375                 380                 


Leu Gly Leu Arg Pro Gly Pro Leu Val Gly Glu Ala Leu Arg Ala Leu 
385                 390                 395                 400 


Leu Glu Ala Gln Ala Glu Gly Ser Val Ala Ser Gln Glu Glu Ala Arg 
                405                 410                 415     


Arg Phe Leu Leu Tyr Trp Arg Asp Gly Arg Thr Ala Gln Ala Thr Pro 
            420                 425                 430         


Ala Pro Asp His Pro Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Cys 
        435                 440                 445             


<210>  149
<211>  448
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Thermus aquaticus poly(A) polymerase 439(flex linker)C

<400>  149

Met Leu Arg Leu Ile Ala His Arg Asp Phe Pro Phe Tyr Thr Pro Lys 
1               5                   10                  15      


Gly Ala Ile Pro Val Gly Gly Ala Leu Arg Asp Leu Leu Leu Gly Glu 
            20                  25                  30          


Arg Pro Leu Asp Leu Asp Phe Ala Ala Trp Asp Pro Glu Lys Ala Ala 
        35                  40                  45              


Glu Glu Ala Lys Gly Arg Tyr Gly Gly Thr Leu Phe Pro Leu Asp Pro 
    50                  55                  60                  


Glu Arg Gly His Tyr Arg Leu Val Ala Gly Ala Leu Thr Leu Asp Phe 
65                  70                  75                  80  


Thr Pro Leu Glu Gly Ser Pro Glu Glu Asp Leu Leu Arg Arg Asp Tyr 
                85                  90                  95      


Arg Val Asn Ala Leu Leu Trp Leu Lys Gly Arg Val Phe Gly Leu Pro 
            100                 105                 110         


Gly Ala Glu Glu Asp Leu Asn Arg Arg Leu Leu Val Pro Val Gln Glu 
        115                 120                 125             


Glu Asn Leu Tyr Ala Asp Pro Leu Arg Ser Leu Arg Gly Val Arg Leu 
    130                 135                 140                 


Ala Ala Thr Leu Gly Leu Gly Leu Pro Pro Glu Thr Arg Arg Ala Leu 
145                 150                 155                 160 


Ala Arg His Ala Arg Phe Leu Gln Glu Asn Pro Ser Ala Leu Pro Ala 
                165                 170                 175     


Arg Glu Arg Val Arg Glu Glu Leu Ala Lys Leu Leu Leu Cys Lys Arg 
            180                 185                 190         


Ala Pro Phe Gly Leu His Leu Leu Glu Arg Thr Gly Leu Leu Gly Ile 
        195                 200                 205             


Tyr Leu Pro Glu Leu Thr His Leu Val Gly Leu His Gln Gly Gly Val 
    210                 215                 220                 


His His Leu Pro Ala Trp Glu His Thr Leu Ser Val Leu Phe His Leu 
225                 230                 235                 240 


Leu Trp Leu Trp Pro Glu Ala Pro Leu Glu Ala Arg Leu Ala Ala Leu 
                245                 250                 255     


Tyr His Asp Ala Gly Lys Pro Leu Thr Arg Arg Tyr Asp Pro Glu Val 
            260                 265                 270         


Gly Arg Tyr Arg Phe Phe Gly His Ala Glu Val Gly Ala Glu Met Ala 
        275                 280                 285             


Lys Ala Ala Leu Phe Trp Leu Arg Phe Pro Lys Glu Val Val Glu Lys 
    290                 295                 300                 


Ala Ala His Leu Val Arg Arg His Met Asp Arg Pro Pro Glu Gly Thr 
305                 310                 315                 320 


Lys Ala Leu Arg Arg Phe Tyr Phe Lys Arg Arg Asp Leu Leu Pro Ala 
                325                 330                 335     


Leu Pro Tyr Leu Met Ala Ala Asp Arg Leu Ala Ala Lys Gly Val Glu 
            340                 345                 350         


Ala Glu Ala Trp Pro Leu Leu Glu Ala Tyr Arg Glu Ala Leu Lys Thr 
        355                 360                 365             


Pro Leu Pro Gln Glu Pro Leu Leu Ser Gly Glu Glu Val Met Ala Leu 
    370                 375                 380                 


Leu Gly Leu Arg Pro Gly Pro Leu Val Gly Glu Ala Leu Arg Ala Leu 
385                 390                 395                 400 


Leu Glu Ala Gln Ala Glu Gly Ser Val Ala Ser Gln Glu Glu Ala Arg 
                405                 410                 415     


Arg Phe Leu Leu Tyr Trp Arg Asp Gly Arg Thr Ala Gln Ala Thr Pro 
            420                 425                 430         


Ala Pro Asp His Pro Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Cys 
        435                 440                 445             


<210>  150
<211>  455
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Thermus aquaticus poly(A) polymerase C190A 439(flex linker+HIS)C

<400>  150

Met Leu Arg Leu Ile Ala His Arg Asp Phe Pro Phe Tyr Thr Pro Lys 
1               5                   10                  15      


Gly Ala Ile Pro Val Gly Gly Ala Leu Arg Asp Leu Leu Leu Gly Glu 
            20                  25                  30          


Arg Pro Leu Asp Leu Asp Phe Ala Ala Trp Asp Pro Glu Lys Ala Ala 
        35                  40                  45              


Glu Glu Ala Lys Gly Arg Tyr Gly Gly Thr Leu Phe Pro Leu Asp Pro 
    50                  55                  60                  


Glu Arg Gly His Tyr Arg Leu Val Ala Gly Ala Leu Thr Leu Asp Phe 
65                  70                  75                  80  


Thr Pro Leu Glu Gly Ser Pro Glu Glu Asp Leu Leu Arg Arg Asp Tyr 
                85                  90                  95      


Arg Val Asn Ala Leu Leu Trp Leu Lys Gly Arg Val Phe Gly Leu Pro 
            100                 105                 110         


Gly Ala Glu Glu Asp Leu Asn Arg Arg Leu Leu Val Pro Val Gln Glu 
        115                 120                 125             


Glu Asn Leu Tyr Ala Asp Pro Leu Arg Ser Leu Arg Gly Val Arg Leu 
    130                 135                 140                 


Ala Ala Thr Leu Gly Leu Gly Leu Pro Pro Glu Thr Arg Arg Ala Leu 
145                 150                 155                 160 


Ala Arg His Ala Arg Phe Leu Gln Glu Asn Pro Ser Ala Leu Pro Ala 
                165                 170                 175     


Arg Glu Arg Val Arg Glu Glu Leu Ala Lys Leu Leu Leu Ala Lys Arg 
            180                 185                 190         


Ala Pro Phe Gly Leu His Leu Leu Glu Arg Thr Gly Leu Leu Gly Ile 
        195                 200                 205             


Tyr Leu Pro Glu Leu Thr His Leu Val Gly Leu His Gln Gly Gly Val 
    210                 215                 220                 


His His Leu Pro Ala Trp Glu His Thr Leu Ser Val Leu Phe His Leu 
225                 230                 235                 240 


Leu Trp Leu Trp Pro Glu Ala Pro Leu Glu Ala Arg Leu Ala Ala Leu 
                245                 250                 255     


Tyr His Asp Ala Gly Lys Pro Leu Thr Arg Arg Tyr Asp Pro Glu Val 
            260                 265                 270         


Gly Arg Tyr Arg Phe Phe Gly His Ala Glu Val Gly Ala Glu Met Ala 
        275                 280                 285             


Lys Ala Ala Leu Phe Trp Leu Arg Phe Pro Lys Glu Val Val Glu Lys 
    290                 295                 300                 


Ala Ala His Leu Val Arg Arg His Met Asp Arg Pro Pro Glu Gly Thr 
305                 310                 315                 320 


Lys Ala Leu Arg Arg Phe Tyr Phe Lys Arg Arg Asp Leu Leu Pro Ala 
                325                 330                 335     


Leu Pro Tyr Leu Met Ala Ala Asp Arg Leu Ala Ala Lys Gly Val Glu 
            340                 345                 350         


Ala Glu Ala Trp Pro Leu Leu Glu Ala Tyr Arg Glu Ala Leu Lys Thr 
        355                 360                 365             


Pro Leu Pro Gln Glu Pro Leu Leu Ser Gly Glu Glu Val Met Ala Leu 
    370                 375                 380                 


Leu Gly Leu Arg Pro Gly Pro Leu Val Gly Glu Ala Leu Arg Ala Leu 
385                 390                 395                 400 


Leu Glu Ala Gln Ala Glu Gly Ser Val Ala Ser Gln Glu Glu Ala Arg 
                405                 410                 415     


Arg Phe Leu Leu Tyr Trp Arg Asp Gly Arg Thr Ala Gln Ala Thr Pro 
            420                 425                 430         


Ala Pro Asp His Pro Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
        435                 440                 445             


His His His His His His Cys 
    450                 455 


<210>  151
<211>  455
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Thermus aquaticus poly(A) polymerase C190S 439(flex linker+HIS)C

<400>  151

Met Leu Arg Leu Ile Ala His Arg Asp Phe Pro Phe Tyr Thr Pro Lys 
1               5                   10                  15      


Gly Ala Ile Pro Val Gly Gly Ala Leu Arg Asp Leu Leu Leu Gly Glu 
            20                  25                  30          


Arg Pro Leu Asp Leu Asp Phe Ala Ala Trp Asp Pro Glu Lys Ala Ala 
        35                  40                  45              


Glu Glu Ala Lys Gly Arg Tyr Gly Gly Thr Leu Phe Pro Leu Asp Pro 
    50                  55                  60                  


Glu Arg Gly His Tyr Arg Leu Val Ala Gly Ala Leu Thr Leu Asp Phe 
65                  70                  75                  80  


Thr Pro Leu Glu Gly Ser Pro Glu Glu Asp Leu Leu Arg Arg Asp Tyr 
                85                  90                  95      


Arg Val Asn Ala Leu Leu Trp Leu Lys Gly Arg Val Phe Gly Leu Pro 
            100                 105                 110         


Gly Ala Glu Glu Asp Leu Asn Arg Arg Leu Leu Val Pro Val Gln Glu 
        115                 120                 125             


Glu Asn Leu Tyr Ala Asp Pro Leu Arg Ser Leu Arg Gly Val Arg Leu 
    130                 135                 140                 


Ala Ala Thr Leu Gly Leu Gly Leu Pro Pro Glu Thr Arg Arg Ala Leu 
145                 150                 155                 160 


Ala Arg His Ala Arg Phe Leu Gln Glu Asn Pro Ser Ala Leu Pro Ala 
                165                 170                 175     


Arg Glu Arg Val Arg Glu Glu Leu Ala Lys Leu Leu Leu Ser Lys Arg 
            180                 185                 190         


Ala Pro Phe Gly Leu His Leu Leu Glu Arg Thr Gly Leu Leu Gly Ile 
        195                 200                 205             


Tyr Leu Pro Glu Leu Thr His Leu Val Gly Leu His Gln Gly Gly Val 
    210                 215                 220                 


His His Leu Pro Ala Trp Glu His Thr Leu Ser Val Leu Phe His Leu 
225                 230                 235                 240 


Leu Trp Leu Trp Pro Glu Ala Pro Leu Glu Ala Arg Leu Ala Ala Leu 
                245                 250                 255     


Tyr His Asp Ala Gly Lys Pro Leu Thr Arg Arg Tyr Asp Pro Glu Val 
            260                 265                 270         


Gly Arg Tyr Arg Phe Phe Gly His Ala Glu Val Gly Ala Glu Met Ala 
        275                 280                 285             


Lys Ala Ala Leu Phe Trp Leu Arg Phe Pro Lys Glu Val Val Glu Lys 
    290                 295                 300                 


Ala Ala His Leu Val Arg Arg His Met Asp Arg Pro Pro Glu Gly Thr 
305                 310                 315                 320 


Lys Ala Leu Arg Arg Phe Tyr Phe Lys Arg Arg Asp Leu Leu Pro Ala 
                325                 330                 335     


Leu Pro Tyr Leu Met Ala Ala Asp Arg Leu Ala Ala Lys Gly Val Glu 
            340                 345                 350         


Ala Glu Ala Trp Pro Leu Leu Glu Ala Tyr Arg Glu Ala Leu Lys Thr 
        355                 360                 365             


Pro Leu Pro Gln Glu Pro Leu Leu Ser Gly Glu Glu Val Met Ala Leu 
    370                 375                 380                 


Leu Gly Leu Arg Pro Gly Pro Leu Val Gly Glu Ala Leu Arg Ala Leu 
385                 390                 395                 400 


Leu Glu Ala Gln Ala Glu Gly Ser Val Ala Ser Gln Glu Glu Ala Arg 
                405                 410                 415     


Arg Phe Leu Leu Tyr Trp Arg Asp Gly Arg Thr Ala Gln Ala Thr Pro 
            420                 425                 430         


Ala Pro Asp His Pro Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
        435                 440                 445             


His His His His His His Cys 
    450                 455 


<210>  152
<211>  455
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Thermus aquaticus poly(A) polymerase C190V 439(flex linker+HIS)C

<400>  152

Met Leu Arg Leu Ile Ala His Arg Asp Phe Pro Phe Tyr Thr Pro Lys 
1               5                   10                  15      


Gly Ala Ile Pro Val Gly Gly Ala Leu Arg Asp Leu Leu Leu Gly Glu 
            20                  25                  30          


Arg Pro Leu Asp Leu Asp Phe Ala Ala Trp Asp Pro Glu Lys Ala Ala 
        35                  40                  45              


Glu Glu Ala Lys Gly Arg Tyr Gly Gly Thr Leu Phe Pro Leu Asp Pro 
    50                  55                  60                  


Glu Arg Gly His Tyr Arg Leu Val Ala Gly Ala Leu Thr Leu Asp Phe 
65                  70                  75                  80  


Thr Pro Leu Glu Gly Ser Pro Glu Glu Asp Leu Leu Arg Arg Asp Tyr 
                85                  90                  95      


Arg Val Asn Ala Leu Leu Trp Leu Lys Gly Arg Val Phe Gly Leu Pro 
            100                 105                 110         


Gly Ala Glu Glu Asp Leu Asn Arg Arg Leu Leu Val Pro Val Gln Glu 
        115                 120                 125             


Glu Asn Leu Tyr Ala Asp Pro Leu Arg Ser Leu Arg Gly Val Arg Leu 
    130                 135                 140                 


Ala Ala Thr Leu Gly Leu Gly Leu Pro Pro Glu Thr Arg Arg Ala Leu 
145                 150                 155                 160 


Ala Arg His Ala Arg Phe Leu Gln Glu Asn Pro Ser Ala Leu Pro Ala 
                165                 170                 175     


Arg Glu Arg Val Arg Glu Glu Leu Ala Lys Leu Leu Leu Val Lys Arg 
            180                 185                 190         


Ala Pro Phe Gly Leu His Leu Leu Glu Arg Thr Gly Leu Leu Gly Ile 
        195                 200                 205             


Tyr Leu Pro Glu Leu Thr His Leu Val Gly Leu His Gln Gly Gly Val 
    210                 215                 220                 


His His Leu Pro Ala Trp Glu His Thr Leu Ser Val Leu Phe His Leu 
225                 230                 235                 240 


Leu Trp Leu Trp Pro Glu Ala Pro Leu Glu Ala Arg Leu Ala Ala Leu 
                245                 250                 255     


Tyr His Asp Ala Gly Lys Pro Leu Thr Arg Arg Tyr Asp Pro Glu Val 
            260                 265                 270         


Gly Arg Tyr Arg Phe Phe Gly His Ala Glu Val Gly Ala Glu Met Ala 
        275                 280                 285             


Lys Ala Ala Leu Phe Trp Leu Arg Phe Pro Lys Glu Val Val Glu Lys 
    290                 295                 300                 


Ala Ala His Leu Val Arg Arg His Met Asp Arg Pro Pro Glu Gly Thr 
305                 310                 315                 320 


Lys Ala Leu Arg Arg Phe Tyr Phe Lys Arg Arg Asp Leu Leu Pro Ala 
                325                 330                 335     


Leu Pro Tyr Leu Met Ala Ala Asp Arg Leu Ala Ala Lys Gly Val Glu 
            340                 345                 350         


Ala Glu Ala Trp Pro Leu Leu Glu Ala Tyr Arg Glu Ala Leu Lys Thr 
        355                 360                 365             


Pro Leu Pro Gln Glu Pro Leu Leu Ser Gly Glu Glu Val Met Ala Leu 
    370                 375                 380                 


Leu Gly Leu Arg Pro Gly Pro Leu Val Gly Glu Ala Leu Arg Ala Leu 
385                 390                 395                 400 


Leu Glu Ala Gln Ala Glu Gly Ser Val Ala Ser Gln Glu Glu Ala Arg 
                405                 410                 415     


Arg Phe Leu Leu Tyr Trp Arg Asp Gly Arg Thr Ala Gln Ala Thr Pro 
            420                 425                 430         


Ala Pro Asp His Pro Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
        435                 440                 445             


His His His His His His Cys 
    450                 455 


<210>  153
<211>  455
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Thermus aquaticus poly(A) polymerase 439(flex linker+HIS)C

<400>  153

Met Leu Arg Leu Ile Ala His Arg Asp Phe Pro Phe Tyr Thr Pro Lys 
1               5                   10                  15      


Gly Ala Ile Pro Val Gly Gly Ala Leu Arg Asp Leu Leu Leu Gly Glu 
            20                  25                  30          


Arg Pro Leu Asp Leu Asp Phe Ala Ala Trp Asp Pro Glu Lys Ala Ala 
        35                  40                  45              


Glu Glu Ala Lys Gly Arg Tyr Gly Gly Thr Leu Phe Pro Leu Asp Pro 
    50                  55                  60                  


Glu Arg Gly His Tyr Arg Leu Val Ala Gly Ala Leu Thr Leu Asp Phe 
65                  70                  75                  80  


Thr Pro Leu Glu Gly Ser Pro Glu Glu Asp Leu Leu Arg Arg Asp Tyr 
                85                  90                  95      


Arg Val Asn Ala Leu Leu Trp Leu Lys Gly Arg Val Phe Gly Leu Pro 
            100                 105                 110         


Gly Ala Glu Glu Asp Leu Asn Arg Arg Leu Leu Val Pro Val Gln Glu 
        115                 120                 125             


Glu Asn Leu Tyr Ala Asp Pro Leu Arg Ser Leu Arg Gly Val Arg Leu 
    130                 135                 140                 


Ala Ala Thr Leu Gly Leu Gly Leu Pro Pro Glu Thr Arg Arg Ala Leu 
145                 150                 155                 160 


Ala Arg His Ala Arg Phe Leu Gln Glu Asn Pro Ser Ala Leu Pro Ala 
                165                 170                 175     


Arg Glu Arg Val Arg Glu Glu Leu Ala Lys Leu Leu Leu Cys Lys Arg 
            180                 185                 190         


Ala Pro Phe Gly Leu His Leu Leu Glu Arg Thr Gly Leu Leu Gly Ile 
        195                 200                 205             


Tyr Leu Pro Glu Leu Thr His Leu Val Gly Leu His Gln Gly Gly Val 
    210                 215                 220                 


His His Leu Pro Ala Trp Glu His Thr Leu Ser Val Leu Phe His Leu 
225                 230                 235                 240 


Leu Trp Leu Trp Pro Glu Ala Pro Leu Glu Ala Arg Leu Ala Ala Leu 
                245                 250                 255     


Tyr His Asp Ala Gly Lys Pro Leu Thr Arg Arg Tyr Asp Pro Glu Val 
            260                 265                 270         


Gly Arg Tyr Arg Phe Phe Gly His Ala Glu Val Gly Ala Glu Met Ala 
        275                 280                 285             


Lys Ala Ala Leu Phe Trp Leu Arg Phe Pro Lys Glu Val Val Glu Lys 
    290                 295                 300                 


Ala Ala His Leu Val Arg Arg His Met Asp Arg Pro Pro Glu Gly Thr 
305                 310                 315                 320 


Lys Ala Leu Arg Arg Phe Tyr Phe Lys Arg Arg Asp Leu Leu Pro Ala 
                325                 330                 335     


Leu Pro Tyr Leu Met Ala Ala Asp Arg Leu Ala Ala Lys Gly Val Glu 
            340                 345                 350         


Ala Glu Ala Trp Pro Leu Leu Glu Ala Tyr Arg Glu Ala Leu Lys Thr 
        355                 360                 365             


Pro Leu Pro Gln Glu Pro Leu Leu Ser Gly Glu Glu Val Met Ala Leu 
    370                 375                 380                 


Leu Gly Leu Arg Pro Gly Pro Leu Val Gly Glu Ala Leu Arg Ala Leu 
385                 390                 395                 400 


Leu Glu Ala Gln Ala Glu Gly Ser Val Ala Ser Gln Glu Glu Ala Arg 
                405                 410                 415     


Arg Phe Leu Leu Tyr Trp Arg Asp Gly Arg Thr Ala Gln Ala Thr Pro 
            420                 425                 430         


Ala Pro Asp His Pro Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
        435                 440                 445             


His His His His His His Cys 
    450                 455 


<210>  154
<211>  446
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Meiothermus silvanus polynucleotide adenylyltransferase 436 (flex
       linker)C

<400>  154

Met Leu Val Ala Ile Arg Arg Val His Leu Ser Pro Phe Pro Phe Pro 
1               5                   10                  15      


Ala Gln Gly Tyr Leu Val Gly Gly Ala Val Arg Asp Leu Met Leu Gly 
            20                  25                  30          


Lys Arg Pro Lys Asp Leu Asp Phe Val Val Pro Asn Pro Glu Glu Ala 
        35                  40                  45              


Ala Arg Asp Leu Ala His Arg Leu Gly Gly Ser Ile Phe Pro Leu Asp 
    50                  55                  60                  


Glu Lys Arg Arg Phe Trp Arg Val Val Ala Pro Gly Thr Thr Tyr Asp 
65                  70                  75                  80  


Tyr Ala Pro Met Pro Gln Gly Leu Glu Val Asp Leu Leu Arg Arg Asp 
                85                  90                  95      


Phe Thr Leu Asn Ala Leu Ala Val Asn Glu Gln Gly Leu Val Leu Gly 
            100                 105                 110         


Leu Pro Ile Ala Arg Tyr Asp Leu Glu His Arg Leu Leu Arg Ala Ile 
        115                 120                 125             


Arg Lys Ala Asn Leu Trp Glu Asp Pro Leu Arg Ser Leu Arg Ala Val 
    130                 135                 140                 


Arg Ile Thr Leu Thr Ala Gly Leu Arg Leu Glu Pro His Thr Glu Gly 
145                 150                 155                 160 


Trp Ile Tyr Glu His Ala Gln Tyr Leu Arg Glu Ser Gly Ala Leu Pro 
                165                 170                 175     


Ala Trp Glu Arg Val Gly Glu Glu Leu Ser Gln Ile Leu Arg His Pro 
            180                 185                 190         


Ala Ala Ala Val Gly Phe Leu Arg Leu Glu Arg Ala Gly Leu Leu Glu 
        195                 200                 205             


Val Tyr Leu Pro Glu Leu Ala Ala Gly Ile Gly Val Glu Gln His Gly 
    210                 215                 220                 


Ala His His Leu Asp Val Trp Arg His Ser Leu Glu Val Leu Ala Gln 
225                 230                 235                 240 


Leu Thr Leu Leu Arg Pro Asp Ala His Leu Ala Leu Arg Trp Ala Ala 
                245                 250                 255     


Leu Leu His Asp Val Ala Lys Pro Gln Thr Arg Gln Trp Ser Pro Glu 
            260                 265                 270         


Lys Gly Arg Tyr Thr Phe Tyr Gly His Asp Ala Leu Gly Ala Glu Gln 
        275                 280                 285             


Ala Arg Gln Ile Leu Asn Arg Leu Arg Gln Pro Ser Glu Val Val Ala 
    290                 295                 300                 


Glu Ala Val Arg Leu Val Arg Leu His Met Gln His Pro Pro Gln Gly 
305                 310                 315                 320 


Gly Arg Glu Leu Arg Arg Phe Leu His Arg Arg Arg Glu Ala Leu Pro 
                325                 330                 335     


Glu Leu Ile Trp Leu Gln Leu Ala Asp Met Thr Gly Ile Leu Gly Tyr 
            340                 345                 350         


Arg Glu Arg Val Glu Glu Leu Arg Glu Gln Leu Gln Ala Ile Glu Ser 
        355                 360                 365             


Leu Ala Glu Gly Thr Pro Ser Pro Lys Pro Leu Leu Asp Gly Arg Glu 
    370                 375                 380                 


Val Met Arg Leu Leu Gly Leu Glu Pro Gly Pro Lys Val Gly Glu Ala 
385                 390                 395                 400 


Leu Ala Ala Leu Leu Glu Ala Gln Ala Ile Gly Glu Val Gln Ser Arg 
                405                 410                 415     


Thr Glu Ala Ala Ser Phe Leu Arg Asp Ala Phe Gly Glu Asp Lys Gly 
            420                 425                 430         


Arg Met Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Cys 
        435                 440                 445     


<210>  155
<211>  452
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Meiothermus silvanus polynucleotide adenylyltransferase 436 (flex
       linker+HIS)C

<400>  155

Met Leu Val Ala Ile Arg Arg Val His Leu Ser Pro Phe Pro Phe Pro 
1               5                   10                  15      


Ala Gln Gly Tyr Leu Val Gly Gly Ala Val Arg Asp Leu Met Leu Gly 
            20                  25                  30          


Lys Arg Pro Lys Asp Leu Asp Phe Val Val Pro Asn Pro Glu Glu Ala 
        35                  40                  45              


Ala Arg Asp Leu Ala His Arg Leu Gly Gly Ser Ile Phe Pro Leu Asp 
    50                  55                  60                  


Glu Lys Arg Arg Phe Trp Arg Val Val Ala Pro Gly Thr Thr Tyr Asp 
65                  70                  75                  80  


Tyr Ala Pro Met Pro Gln Gly Leu Glu Val Asp Leu Leu Arg Arg Asp 
                85                  90                  95      


Phe Thr Leu Asn Ala Leu Ala Val Asn Glu Gln Gly Leu Val Leu Gly 
            100                 105                 110         


Leu Pro Ile Ala Arg Tyr Asp Leu Glu His Arg Leu Leu Arg Ala Ile 
        115                 120                 125             


Arg Lys Ala Asn Leu Trp Glu Asp Pro Leu Arg Ser Leu Arg Ala Val 
    130                 135                 140                 


Arg Ile Thr Leu Thr Ala Gly Leu Arg Leu Glu Pro His Thr Glu Gly 
145                 150                 155                 160 


Trp Ile Tyr Glu His Ala Gln Tyr Leu Arg Glu Ser Gly Ala Leu Pro 
                165                 170                 175     


Ala Trp Glu Arg Val Gly Glu Glu Leu Ser Gln Ile Leu Arg His Pro 
            180                 185                 190         


Ala Ala Ala Val Gly Phe Leu Arg Leu Glu Arg Ala Gly Leu Leu Glu 
        195                 200                 205             


Val Tyr Leu Pro Glu Leu Ala Ala Gly Ile Gly Val Glu Gln His Gly 
    210                 215                 220                 


Ala His His Leu Asp Val Trp Arg His Ser Leu Glu Val Leu Ala Gln 
225                 230                 235                 240 


Leu Thr Leu Leu Arg Pro Asp Ala His Leu Ala Leu Arg Trp Ala Ala 
                245                 250                 255     


Leu Leu His Asp Val Ala Lys Pro Gln Thr Arg Gln Trp Ser Pro Glu 
            260                 265                 270         


Lys Gly Arg Tyr Thr Phe Tyr Gly His Asp Ala Leu Gly Ala Glu Gln 
        275                 280                 285             


Ala Arg Gln Ile Leu Asn Arg Leu Arg Gln Pro Ser Glu Val Val Ala 
    290                 295                 300                 


Glu Ala Val Arg Leu Val Arg Leu His Met Gln His Pro Pro Gln Gly 
305                 310                 315                 320 


Gly Arg Glu Leu Arg Arg Phe Leu His Arg Arg Arg Glu Ala Leu Pro 
                325                 330                 335     


Glu Leu Ile Trp Leu Gln Leu Ala Asp Met Thr Gly Ile Leu Gly Tyr 
            340                 345                 350         


Arg Glu Arg Val Glu Glu Leu Arg Glu Gln Leu Gln Ala Ile Glu Ser 
        355                 360                 365             


Leu Ala Glu Gly Thr Pro Ser Pro Lys Pro Leu Leu Asp Gly Arg Glu 
    370                 375                 380                 


Val Met Arg Leu Leu Gly Leu Glu Pro Gly Pro Lys Val Gly Glu Ala 
385                 390                 395                 400 


Leu Ala Ala Leu Leu Glu Ala Gln Ala Ile Gly Glu Val Gln Ser Arg 
                405                 410                 415     


Thr Glu Ala Ala Ser Phe Leu Arg Asp Ala Phe Gly Glu Asp Lys Gly 
            420                 425                 430         


Arg Met Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser His His His 
        435                 440                 445             


His His His Cys 
    450         


<210>  156
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  linker

<400>  156

Ser Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Ser Leu Gln 
            20                  25      


<210>  157
<211>  12
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Rigid-linker_AEAAAKEAAAKA

<400>  157

Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Ala 
1               5                   10          


<210>  158
<211>  46
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Rigid-linker

<400>  158

Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
1               5                   10                  15      


Glu Ala Ala Ala Lys Ala Leu Glu Ala Glu Ala Ala Ala Lys Glu Ala 
            20                  25                  30          


Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Ala 
        35                  40                  45      


<210>  159
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Rigid-linker (EAAAK)1

<400>  159

Glu Ala Ala Ala Lys 
1               5   


<210>  160
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Rigid-linker (EAAAK)2

<400>  160

Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
1               5                   10  


<210>  161
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Rigid-linker (EAAAK)3

<400>  161

Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
1               5                   10                  15  


<210>  162
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Linker-GGG(GC)

<400>  162

Gly Gly Gly Gly 
1               


<210>  163
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Linker-GGGGG(GC)

<400>  163

Gly Gly Gly Gly Gly 
1               5   


<210>  164
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Gly-linker (G)6

<400>  164

Gly Gly Gly Gly Gly Gly 
1               5       


<210>  165
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Gly-linker (G)8

<400>  165

Gly Gly Gly Gly Gly Gly Gly Gly 
1               5               


<210>  166
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Linker GGGS

<400>  166

Gly Gly Gly Gly Ser 
1               5   


<210>  167
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Linker-G4SG

<400>  167

Gly Gly Gly Gly Ser Gly 
1               5       


<210>  168
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Linker-G4SG4(GC)

<400>  168

Gly Gly Gly Gly Ser Gly Gly Gly Gly 
1               5                   


<210>  169
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Linker-(G4S)2

<400>  169

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10  


<210>  170
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Linker (GGGGS)2

<400>  170

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10  


<210>  171
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Linker-(GGGGS)3

<400>  171

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  172
<211>  20
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Linker (GGGGS)4

<400>  172

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser 
            20  


<210>  173
<211>  16
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Linker-GGGSGGGGSGGGGSGG(GC)

<400>  173

Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
1               5                   10                  15      


<210>  174
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Linker-GGSGGGGSGGGGSGG

<400>  174

Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
1               5                   10                  15  


<210>  175
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Linker-GSG

<400>  175

Gly Ser Gly Ser 
1               


<210>  176
<211>  21
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Linker(GC)

<400>  176

Gly Ser Gly Gly Gly Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
1               5                   10                  15      


Gly Gly Gly Gly Ser 
            20      


<210>  177
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Rigid-linker

<400>  177

Pro Ala Pro Ala Pro 
1               5   


<210>  178
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Linker-PPPGSRHDKIH(GC)

<400>  178

Pro Pro Pro Gly Ser Arg His Asp Lys Ile His 
1               5                   10      


<210>  179
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Linker-SGG(GC)

<400>  179

Ser Gly Gly Gly 
1               


<210>  180
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Linker-SGS

<400>  180

Gly Ser Gly Ser 
1               


<210>  181
<211>  17
<212>  PRT
<213>  Artificial sequence

<220>
<223>  19-A

<400>  181

Glu Trp Leu Lys Ala Phe Tyr Glu Lys Val Leu Glu Lys Leu Lys Glu 
1               5                   10                  15      


Leu 
    


<210>  182
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Avi-Tag

<400>  182

Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu 
1               5                   10                  15  


<210>  183
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Calmodulin Tag

<400>  183

Lys Arg Arg Trp Lys Lys Asn Phe Ile Ala Val Ser Ala Ala Asn Arg 
1               5                   10                  15      


Phe Lys Lys Ile Ser Ser Ser Gly Ala Leu 
            20                  25      


<210>  184
<211>  13
<212>  PRT
<213>  Artificial sequence

<220>
<223>  E-tag

<400>  184

Gly Ala Pro Val Pro Tyr Pro Asp Pro Leu Glu Pro Arg 
1               5                   10              


<210>  185
<211>  16
<212>  PRT
<213>  Artificial sequence

<220>
<223>  ELK16-Tag

<400>  185

Leu Glu Leu Glu Leu Lys Leu Lys Leu Glu Leu Glu Leu Lys Leu Lys 
1               5                   10                  15      


<210>  186
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FLAG tag

<400>  186

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  187
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Poly-Glutamate-Tag

<400>  187

Glu Glu Glu Glu Glu Glu 
1               5       


<210>  188
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  HA-tag

<400>  188

Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   


<210>  189
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  HIS tag

<400>  189

His His His His His His 
1               5       


<210>  190
<211>  16
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Isopeptag

<400>  190

Thr Asp Lys Asp Met Thr Ile Thr Phe Thr Asn Lys Lys Asp Ala Glu 
1               5                   10                  15      


<210>  191
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Myc Tag

<400>  191

Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
1               5                   10  


<210>  192
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  S-tag

<400>  192

Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln His Met Asp Ser 
1               5                   10                  15  


<210>  193
<211>  38
<212>  PRT
<213>  Artificial sequence

<220>
<223>  SBP tag

<400>  193

Met Asp Glu Lys Thr Thr Gly Trp Arg Gly Gly His Val Val Glu Gly 
1               5                   10                  15      


Leu Ala Gly Glu Leu Glu Gln Leu Arg Ala Arg Leu Glu His His Pro 
            20                  25                  30          


Gln Gly Gln Arg Glu Pro 
        35              


<210>  194
<211>  13
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Spy Tag

<400>  194

Ala His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys 
1               5                   10              


<210>  195
<211>  13
<212>  PRT
<213>  Artificial sequence

<220>
<223>  SofTag 1

<400>  195

Ser Leu Ala Glu Leu Leu Asn Ala Gly Leu Gly Gly Ser 
1               5                   10              


<210>  196
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Sof Tag 2

<400>  196

Thr Gln Asp Pro Ser Arg Val Gly 
1               5               


<210>  197
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Strep-II-Tag

<400>  197

Trp Ser His Pro Gln Phe Glu Lys 
1               5               


<210>  198
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T7 Epitope tag

<400>  198

Met Ala Ser Met Thr Gly Gly Gln Gln Met Gly 
1               5                   10      


<210>  199
<211>  14
<212>  PRT
<213>  Artificial sequence

<220>
<223>  V5 Tag

<400>  199

Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr 
1               5                   10                  


<210>  200
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  VSV Tag

<400>  200

Tyr Thr Asp Ile Glu Met Asn Arg Leu Gly Lys 
1               5                   10      


<210>  201
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Xpress Tag

<400>  201

Asp Leu Tyr Asp Asp Asp Asp Lys 
1               5               


<210>  202
<211>  60
<212>  RNA
<213>  Artificial sequence

<220>
<223>  RNA template

<400>  202
gccaccuccg cugaugaggc cucgaccgau aggucgaggu cgaggccgaa aggcagcgcc       60


<210>  203
<211>  471
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Escherichia coli pcnB C88S C256R C346A 455 (flexLinker+HIS)C

<400>  203

Met Leu Ser Arg Glu Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro 
1               5                   10                  15      


Gln Val Thr Val Ile Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp 
            20                  25                  30          


Ile Ser Glu Asn Ala Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly 
        35                  40                  45              


Tyr Glu Ala Trp Leu Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly 
    50                  55                  60                  


Lys Lys Pro Lys Asp Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln 
65                  70                  75                  80  


Val Arg Lys Leu Phe Arg Asn Ser Arg Leu Val Gly Arg Arg Phe Arg 
                85                  90                  95      


Leu Ala His Val Met Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe 
            100                 105                 110         


Arg Gly His His Glu Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg 
        115                 120                 125             


Gly Gln Asn Gly Met Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu 
    130                 135                 140                 


Glu Asp Ala Gln Arg Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser 
145                 150                 155                 160 


Val Ala Asp Phe Thr Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu 
                165                 170                 175     


Lys Asp Gly Val Ile Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg 
            180                 185                 190         


Glu Asp Pro Val Arg Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu 
        195                 200                 205             


Gly Met Arg Ile Ser Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala 
    210                 215                 220                 


Thr Leu Leu Asn Asp Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu 
225                 230                 235                 240 


Lys Leu Leu Gln Ala Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Arg 
                245                 250                 255     


Glu Tyr His Leu Phe Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe 
            260                 265                 270         


Thr Glu Asn Gly Asp Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu 
        275                 280                 285             


Lys Asn Thr Asp Thr Arg Ile His Asn Asp Met Arg Val Asn Pro Ala 
    290                 295                 300                 


Phe Leu Phe Ala Ala Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln 
305                 310                 315                 320 


Lys Ile Ala Gln Glu Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu 
                325                 330                 335     


Ala Met Asn Asp Val Leu Asp Glu Ala Ala Arg Ser Leu Ala Ile Pro 
            340                 345                 350         


Lys Arg Leu Thr Thr Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg 
        355                 360                 365             


Met Ser Arg Arg Gln Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro 
    370                 375                 380                 


Lys Phe Arg Ala Ala Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu 
385                 390                 395                 400 


Arg Asn Ala Glu Leu Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln 
                405                 410                 415     


Val Ser Ala Pro Pro Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu 
            420                 425                 430         


Glu Pro Ser Pro Arg Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro 
        435                 440                 445             


Arg Arg Glu Gly Thr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    450                 455                 460                 


His His His His His His Cys 
465                 470     


