                         SEQUENCE LISTING

<110>  Malteries Soufflet
       INRA
 
<120>  Utilisation de cystine endoprotase pour diminuer le trouble de 
       boissons

<130>  BET16P0699

<150>  FR15/53869
<151>  2015-04-29

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  365
<212>  PRT
<213>  Hordeum vulgare

<400>  1

Met Trp Arg Cys Ile Leu Leu Ser Ala Val Val Val Ala Leu Ala Leu 
1               5                   10                  15      


Ala Pro Ala Pro Ala Leu Gly Val Pro Phe Thr Glu Lys Asp Leu Ala 
            20                  25                  30          


Ser Glu Glu Ser Leu Arg Gly Leu Tyr Glu Arg Trp Arg Ser His Tyr 
        35                  40                  45              


Thr Val Ser Arg Arg Gly Leu Gly Ala Asp Ala Glu Glu Arg Arg Phe 
    50                  55                  60                  


Asn Val Phe Lys Glu Asn Ala Arg Tyr Val His Glu Gly Asn Lys Arg 
65                  70                  75                  80  


Asp Arg Pro Phe Arg Leu Ala Leu Asn Lys Phe Ala Asp Met Thr Thr 
                85                  90                  95      


Asp Glu Phe Arg Arg Thr Tyr Ala Gly Ser Arg Val Arg His His Leu 
            100                 105                 110         


Ser Leu Ser Gly Gly Arg Arg Gly Asp Gly Gly Phe Arg Tyr Ala Asp 
        115                 120                 125             


Ala Asp Asn Leu Pro Pro Ala Val Asp Trp Arg Gln Lys Gly Ala Val 
    130                 135                 140                 


Thr Ala Ile Lys Asp Gln Gly Gln Cys Gly Ser Cys Trp Ala Phe Ser 
145                 150                 155                 160 


Thr Ile Val Ala Val Glu Gly Ile Asn Lys Ile Arg Thr Gly Lys Leu 
                165                 170                 175     


Val Ser Leu Ser Glu Gln Glu Leu Met Asp Cys Asp Asn Val Asn Asn 
            180                 185                 190         


Gln Gly Cys Glu Gly Gly Leu Met Asp Tyr Ala Phe Gln Phe Ile Gln 
        195                 200                 205             


Lys Asn Gly Ile Thr Thr Glu Ser Asn Tyr Pro Tyr Gln Gly Glu Gln 
    210                 215                 220                 


Gly Ser Cys Asp Gln Ala Lys Glu Asn Ala Gln Ala Val Thr Ile Asp 
225                 230                 235                 240 


Gly Tyr Glu Asp Val Pro Ala Asn Asp Glu Ser Ala Leu Gln Lys Ala 
                245                 250                 255     


Val Ala Gly Gln Pro Val Ser Val Ala Ile Asp Ala Ser Gly Gln Asp 
            260                 265                 270         


Phe Gln Phe Tyr Ser Glu Gly Val Phe Thr Gly Glu Cys Ser Thr Asp 
        275                 280                 285             


Leu Asp His Gly Val Ala Ala Val Gly Tyr Gly Ala Thr Arg Asp Gly 
    290                 295                 300                 


Thr Lys Tyr Trp Ile Val Lys Asn Ser Trp Gly Glu Asp Trp Gly Glu 
305                 310                 315                 320 


Lys Gly Tyr Ile Arg Met Gln Arg Gly Val Ser Gln Thr Glu Gly Leu 
                325                 330                 335     


Cys Gly Ile Ala Met Gln Ala Ser Tyr Pro Thr Lys Ser Ala Pro His 
            340                 345                 350         


Ala Ser Thr Val Arg Glu Gly Ser His Thr Asp Glu Leu 
        355                 360                 365 


<210>  2
<211>  365
<212>  PRT
<213>  Hordeum vulgare

<400>  2

Met Trp Arg Cys Ile Leu Leu Ser Ala Val Val Val Ala Leu Ala Leu 
1               5                   10                  15      


Ala Pro Ala Pro Ala Leu Gly Val Pro Phe Thr Glu Lys Asp Leu Ala 
            20                  25                  30          


Ser Glu Glu Ser Leu Arg Gly Leu Tyr Glu Arg Trp Arg Ser His Tyr 
        35                  40                  45              


Thr Val Ser Arg Arg Gly Leu Gly Ala Asp Ala Glu Glu Arg Arg Phe 
    50                  55                  60                  


Asn Val Phe Lys Gln Asn Ala Arg Tyr Val His Glu Gly Asn Lys Arg 
65                  70                  75                  80  


Asp Met Pro Phe Arg Leu Ala Leu Asn Lys Phe Ala Asp Met Thr Thr 
                85                  90                  95      


Asp Glu Phe Arg Arg Thr Tyr Ala Gly Ser Arg Val Arg His His Leu 
            100                 105                 110         


Ser Leu Ser Gly Gly Arg Arg Gly Asp Gly Gly Phe Arg Tyr Gly Asp 
        115                 120                 125             


Ala Asp Asn Leu Pro Pro Ala Val Asp Trp Arg Gln Lys Gly Ala Val 
    130                 135                 140                 


Thr Ala Ile Lys Asp Gln Gly Gln Cys Gly Ser Cys Trp Ala Phe Ser 
145                 150                 155                 160 


Thr Ile Val Ala Val Glu Gly Ile Asn Lys Ile Arg Thr Gly Lys Leu 
                165                 170                 175     


Val Ser Leu Ser Glu Gln Glu Leu Met Asp Cys Asp Asn Val Asn Asn 
            180                 185                 190         


Gln Gly Cys Asp Gly Gly Leu Met Asp Tyr Ala Phe Gln Phe Ile Gln 
        195                 200                 205             


Lys Asn Gly Ile Thr Thr Glu Ser Asn Tyr Pro Tyr Gln Gly Glu Gln 
    210                 215                 220                 


Gly Ser Cys Asp Gln Ala Lys Glu Asn Ala Gln Ala Val Thr Ile Asp 
225                 230                 235                 240 


Gly Tyr Glu Asp Val Pro Ala Asn Asp Glu Ser Ala Leu Gln Lys Ala 
                245                 250                 255     


Val Ala Gly Gln Pro Val Ser Val Ala Ile Asp Ala Ser Gly Gln Asp 
            260                 265                 270         


Phe Gln Phe Tyr Ser Glu Gly Val Phe Thr Gly Glu Cys Ser Thr Asp 
        275                 280                 285             


Leu Asp His Gly Val Ala Ala Val Gly Tyr Gly Ala Thr Arg Asp Gly 
    290                 295                 300                 


Thr Lys Tyr Trp Ile Val Lys Asn Ser Trp Gly Glu Asp Trp Gly Glu 
305                 310                 315                 320 


Lys Gly Tyr Ile Arg Met Gln Arg Gly Val Ser Gln Thr Glu Gly Leu 
                325                 330                 335     


Cys Gly Ile Ala Met Gln Ala Ser Tyr Pro Thr Lys Ser Ala Pro His 
            340                 345                 350         


Ala Ser Thr Val Arg Glu Glu Ser His Thr Asp Glu Leu 
        355                 360                 365 


<210>  3
<211>  371
<212>  PRT
<213>  Hordeum vulgare

<400>  3

Met Gly Leu Leu Ser Lys Lys Leu Leu Val Ala Ser Met Val Ala Ala 
1               5                   10                  15      


Val Leu Ala Val Ala Ala Val Glu Leu Cys Ser Ala Ile Pro Met Glu 
            20                  25                  30          


Asp Lys Asp Leu Glu Ser Glu Glu Ala Leu Trp Asp Leu Tyr Glu Arg 
        35                  40                  45              


Trp Gln Ser Ala His Arg Val Arg Arg His His Ala Glu Lys His Arg 
    50                  55                  60                  


Arg Phe Gly Thr Phe Lys Ser Asn Ala His Phe Ile His Ser His Asn 
65                  70                  75                  80  


Lys Arg Gly Asp His Pro Tyr Arg Leu His Leu Asn Arg Phe Gly Asp 
                85                  90                  95      


Met Asp Gln Ala Glu Phe Arg Ala Thr Phe Val Gly Asp Leu Arg Arg 
            100                 105                 110         


Asp Thr Pro Ala Lys Pro Pro Ser Val Pro Gly Phe Met Tyr Ala Ala 
        115                 120                 125             


Leu Asn Val Ser Asp Leu Pro Pro Ser Val Asp Trp Arg Gln Lys Gly 
    130                 135                 140                 


Ala Val Thr Gly Val Lys Asp Gln Gly Lys Cys Gly Ser Cys Trp Ala 
145                 150                 155                 160 


Phe Ser Thr Val Val Ser Val Glu Gly Ile Asn Ala Ile Arg Thr Gly 
                165                 170                 175     


Ser Leu Val Ser Leu Ser Glu Gln Glu Leu Ile Asp Cys Asp Thr Ala 
            180                 185                 190         


Asp Asn Asp Gly Cys Gln Gly Gly Leu Met Asp Asn Ala Phe Glu Tyr 
        195                 200                 205             


Ile Lys Asn Asn Gly Gly Leu Ile Thr Glu Ala Ala Tyr Pro Tyr Arg 
    210                 215                 220                 


Ala Ala Arg Gly Thr Cys Asn Val Ala Arg Ala Ala Gln Asn Ser Pro 
225                 230                 235                 240 


Val Val Val His Ile Asp Gly His Gln Asp Val Pro Ala Asn Ser Glu 
                245                 250                 255     


Glu Asp Leu Ala Arg Ala Val Ala Asn Gln Pro Val Ser Val Ala Val 
            260                 265                 270         


Glu Ala Ser Gly Lys Ala Phe Met Phe Tyr Ser Glu Gly Val Phe Thr 
        275                 280                 285             


Gly Asp Cys Gly Thr Glu Leu Asp His Gly Val Ala Val Val Gly Tyr 
    290                 295                 300                 


Gly Val Ala Glu Asp Gly Lys Ala Tyr Trp Thr Val Lys Asn Ser Trp 
305                 310                 315                 320 


Gly Pro Ser Trp Gly Glu Gln Gly Tyr Ile Arg Val Glu Lys Asp Ser 
                325                 330                 335     


Gly Ala Ser Gly Gly Leu Cys Gly Ile Ala Met Glu Ala Ser Tyr Pro 
            340                 345                 350         


Val Lys Thr Tyr Asn Lys Pro Met Pro Arg Arg Ala Leu Gly Ala Trp 
        355                 360                 365             


Glu Ser Gln 
    370     


<210>  4
<211>  373
<212>  PRT
<213>  Hordeum vulgare

<400>  4

Met Gly Leu Leu Ser Lys Lys Leu Leu Val Ala Ser Met Val Ala Ala 
1               5                   10                  15      


Val Leu Ala Val Ala Ala Val Glu Leu Cys Ser Ala Ile Pro Met Glu 
            20                  25                  30          


Asp Lys Asp Leu Glu Ser Glu Glu Ala Leu Trp Asp Leu Tyr Glu Arg 
        35                  40                  45              


Trp Gln Ser Ala His Arg Val Arg Arg His His Ala Glu Lys His Arg 
    50                  55                  60                  


Arg Phe Gly Thr Phe Lys Ser Asn Ala His Phe Ile His Ser His Asn 
65                  70                  75                  80  


Lys Arg Gly Asp His Pro Tyr Arg Leu His Leu Asn Arg Phe Gly Asp 
                85                  90                  95      


Met Asp Gln Ala Glu Phe Arg Ala Thr Phe Val Gly Asp Leu Arg Arg 
            100                 105                 110         


Asp Thr Pro Ser Lys Pro Pro Ser Val Pro Gly Phe Met Tyr Ala Ala 
        115                 120                 125             


Leu Asn Val Ser Asp Leu Pro Pro Ser Val Asp Trp Arg Gln Lys Gly 
    130                 135                 140                 


Ala Val Thr Gly Val Lys Asp Gln Gly Lys Cys Gly Ser Cys Trp Ala 
145                 150                 155                 160 


Phe Ser Thr Val Val Ser Val Glu Gly Ile Asn Ala Ile Arg Thr Gly 
                165                 170                 175     


Ser Leu Val Ser Leu Ser Glu Gln Glu Leu Ile Asp Cys Asp Thr Ala 
            180                 185                 190         


Asp Asn Asp Gly Cys Gln Gly Gly Leu Met Asp Asn Ala Phe Glu Tyr 
        195                 200                 205             


Ile Lys Asn Asn Gly Gly Leu Ile Thr Glu Ala Ala Tyr Pro Tyr Arg 
    210                 215                 220                 


Ala Ala Arg Gly Thr Cys Asn Val Ala Arg Ala Ala Gln Asn Ser Pro 
225                 230                 235                 240 


Val Val Val His Ile Asp Gly His Gln Asp Val Pro Ala Asn Ser Glu 
                245                 250                 255     


Glu Asp Leu Ala Arg Ala Val Ala Asn Gln Pro Val Ser Val Ala Val 
            260                 265                 270         


Glu Ala Ser Gly Lys Ala Phe Met Phe Tyr Ser Glu Gly Val Phe Thr 
        275                 280                 285             


Gly Glu Cys Gly Thr Glu Leu Asp His Gly Val Ala Val Val Gly Tyr 
    290                 295                 300                 


Gly Val Ala Glu Asp Gly Lys Ala Tyr Trp Thr Val Lys Asn Ser Trp 
305                 310                 315                 320 


Gly Pro Ser Trp Gly Glu Gln Gly Tyr Ile Arg Val Glu Lys Asp Ser 
                325                 330                 335     


Gly Ala Ser Gly Gly Leu Cys Gly Ile Ala Met Glu Ala Ser Tyr Pro 
            340                 345                 350         


Val Lys Thr Tyr Ser Lys Pro Lys Pro Thr Pro Arg Arg Ala Leu Gly 
        355                 360                 365             


Ala Arg Glu Ser Leu 
    370             


<210>  5
<211>  238
<212>  PRT
<213>  Hordeum vulgare

<400>  5

Asp Ala Asp Asn Leu Pro Pro Ala Val Asp Trp Arg Gln Lys Gly Ala 
1               5                   10                  15      


Val Thr Ala Ile Lys Asp Gln Gly Gln Cys Gly Ser Cys Trp Ala Phe 
            20                  25                  30          


Ser Thr Ile Val Ala Val Glu Gly Ile Asn Lys Ile Arg Thr Gly Lys 
        35                  40                  45              


Leu Val Ser Leu Ser Glu Gln Glu Leu Met Asp Cys Asp Asn Val Asn 
    50                  55                  60                  


Asn Gln Gly Cys Glu Gly Gly Leu Met Asp Tyr Ala Phe Gln Phe Ile 
65                  70                  75                  80  


Gln Lys Asn Gly Ile Thr Thr Glu Ser Asn Tyr Pro Tyr Gln Gly Glu 
                85                  90                  95      


Gln Gly Ser Cys Asp Gln Ala Lys Glu Asn Ala Gln Ala Val Thr Ile 
            100                 105                 110         


Asp Gly Tyr Glu Asp Val Pro Ala Asn Asp Glu Ser Ala Leu Gln Lys 
        115                 120                 125             


Ala Val Ala Gly Gln Pro Val Ser Val Ala Ile Asp Ala Ser Gly Gln 
    130                 135                 140                 


Asp Phe Gln Phe Tyr Ser Glu Gly Val Phe Thr Gly Glu Cys Ser Thr 
145                 150                 155                 160 


Asp Leu Asp His Gly Val Ala Ala Val Gly Tyr Gly Ala Thr Arg Asp 
                165                 170                 175     


Gly Thr Lys Tyr Trp Ile Val Lys Asn Ser Trp Gly Glu Asp Trp Gly 
            180                 185                 190         


Glu Lys Gly Tyr Ile Arg Met Gln Arg Gly Val Ser Gln Thr Glu Gly 
        195                 200                 205             


Leu Cys Gly Ile Ala Met Gln Ala Ser Tyr Pro Thr Lys Ser Ala Pro 
    210                 215                 220                 


His Ala Ser Thr Val Arg Glu Gly Ser His Thr Asp Glu Leu 
225                 230                 235             


<210>  6
<211>  238
<212>  PRT
<213>  Hordeum vulgare

<400>  6

Asp Ala Asp Asn Leu Pro Pro Ala Val Asp Trp Arg Gln Lys Gly Ala 
1               5                   10                  15      


Val Thr Ala Ile Lys Asp Gln Gly Gln Cys Gly Ser Cys Trp Ala Phe 
            20                  25                  30          


Ser Thr Ile Val Ala Val Glu Gly Ile Asn Lys Ile Arg Thr Gly Lys 
        35                  40                  45              


Leu Val Ser Leu Ser Glu Gln Glu Leu Met Asp Cys Asp Asn Val Asn 
    50                  55                  60                  


Asn Gln Gly Cys Asp Gly Gly Leu Met Asp Tyr Ala Phe Gln Phe Ile 
65                  70                  75                  80  


Gln Lys Asn Gly Ile Thr Thr Glu Ser Asn Tyr Pro Tyr Gln Gly Glu 
                85                  90                  95      


Gln Gly Ser Cys Asp Gln Ala Lys Glu Asn Ala Gln Ala Val Thr Ile 
            100                 105                 110         


Asp Gly Tyr Glu Asp Val Pro Ala Asn Asp Glu Ser Ala Leu Gln Lys 
        115                 120                 125             


Ala Val Ala Gly Gln Pro Val Ser Val Ala Ile Asp Ala Ser Gly Gln 
    130                 135                 140                 


Asp Phe Gln Phe Tyr Ser Glu Gly Val Phe Thr Gly Glu Cys Ser Thr 
145                 150                 155                 160 


Asp Leu Asp His Gly Val Ala Ala Val Gly Tyr Gly Ala Thr Arg Asp 
                165                 170                 175     


Gly Thr Lys Tyr Trp Ile Val Lys Asn Ser Trp Gly Glu Asp Trp Gly 
            180                 185                 190         


Glu Lys Gly Tyr Ile Arg Met Gln Arg Gly Val Ser Gln Thr Glu Gly 
        195                 200                 205             


Leu Cys Gly Ile Ala Met Gln Ala Ser Tyr Pro Thr Lys Ser Ala Pro 
    210                 215                 220                 


His Ala Ser Thr Val Arg Glu Glu Ser His Thr Asp Glu Leu 
225                 230                 235             


<210>  7
<211>  241
<212>  PRT
<213>  Hordeum vulgare

<400>  7

Val Ser Asp Leu Pro Pro Ser Val Asp Trp Arg Gln Lys Gly Ala Val 
1               5                   10                  15      


Thr Gly Val Lys Asp Gln Gly Lys Cys Gly Ser Cys Trp Ala Phe Ser 
            20                  25                  30          


Thr Val Val Ser Val Glu Gly Ile Asn Ala Ile Arg Thr Gly Ser Leu 
        35                  40                  45              


Val Ser Leu Ser Glu Gln Glu Leu Ile Asp Cys Asp Thr Ala Asp Asn 
    50                  55                  60                  


Asp Gly Cys Gln Gly Gly Leu Met Asp Asn Ala Phe Glu Tyr Ile Lys 
65                  70                  75                  80  


Asn Asn Gly Gly Leu Ile Thr Glu Ala Ala Tyr Pro Tyr Arg Ala Ala 
                85                  90                  95      


Arg Gly Thr Cys Asn Val Ala Arg Ala Ala Gln Asn Ser Pro Val Val 
            100                 105                 110         


Val His Ile Asp Gly His Gln Asp Val Pro Ala Asn Ser Glu Glu Asp 
        115                 120                 125             


Leu Ala Arg Ala Val Ala Asn Gln Pro Val Ser Val Ala Val Glu Ala 
    130                 135                 140                 


Ser Gly Lys Ala Phe Met Phe Tyr Ser Glu Gly Val Phe Thr Gly Asp 
145                 150                 155                 160 


Cys Gly Thr Glu Leu Asp His Gly Val Ala Val Val Gly Tyr Gly Val 
                165                 170                 175     


Ala Glu Asp Gly Lys Ala Tyr Trp Thr Val Lys Asn Ser Trp Gly Pro 
            180                 185                 190         


Ser Trp Gly Glu Gln Gly Tyr Ile Arg Val Glu Lys Asp Ser Gly Ala 
        195                 200                 205             


Ser Gly Gly Leu Cys Gly Ile Ala Met Glu Ala Ser Tyr Pro Val Lys 
    210                 215                 220                 


Thr Tyr Asn Lys Pro Met Pro Arg Arg Ala Leu Gly Ala Trp Glu Ser 
225                 230                 235                 240 


Gln 
    


<210>  8
<211>  243
<212>  PRT
<213>  Hordeum vulgare

<400>  8

Val Ser Asp Leu Pro Pro Ser Val Asp Trp Arg Gln Lys Gly Ala Val 
1               5                   10                  15      


Thr Gly Val Lys Asp Gln Gly Lys Cys Gly Ser Cys Trp Ala Phe Ser 
            20                  25                  30          


Thr Val Val Ser Val Glu Gly Ile Asn Ala Ile Arg Thr Gly Ser Leu 
        35                  40                  45              


Val Ser Leu Ser Glu Gln Glu Leu Ile Asp Cys Asp Thr Ala Asp Asn 
    50                  55                  60                  


Asp Gly Cys Gln Gly Gly Leu Met Asp Asn Ala Phe Glu Tyr Ile Lys 
65                  70                  75                  80  


Asn Asn Gly Gly Leu Ile Thr Glu Ala Ala Tyr Pro Tyr Arg Ala Ala 
                85                  90                  95      


Arg Gly Thr Cys Asn Val Ala Arg Ala Ala Gln Asn Ser Pro Val Val 
            100                 105                 110         


Val His Ile Asp Gly His Gln Asp Val Pro Ala Asn Ser Glu Glu Asp 
        115                 120                 125             


Leu Ala Arg Ala Val Ala Asn Gln Pro Val Ser Val Ala Val Glu Ala 
    130                 135                 140                 


Ser Gly Lys Ala Phe Met Phe Tyr Ser Glu Gly Val Phe Thr Gly Glu 
145                 150                 155                 160 


Cys Gly Thr Glu Leu Asp His Gly Val Ala Val Val Gly Tyr Gly Val 
                165                 170                 175     


Ala Glu Asp Gly Lys Ala Tyr Trp Thr Val Lys Asn Ser Trp Gly Pro 
            180                 185                 190         


Ser Trp Gly Glu Gln Gly Tyr Ile Arg Val Glu Lys Asp Ser Gly Ala 
        195                 200                 205             


Ser Gly Gly Leu Cys Gly Ile Ala Met Glu Ala Ser Tyr Pro Val Lys 
    210                 215                 220                 


Thr Tyr Ser Lys Pro Lys Pro Thr Pro Arg Arg Ala Leu Gly Ala Arg 
225                 230                 235                 240 


Glu Ser Leu 
            


