                         SEQUENCE LISTING

<110>  President and Fellows of Harvard College
 
<120>  INSULIN-DEGRADING ENZYME (IDE) INHIBITORS AND USES THEREOF

<130>  H0824.70206WO00

<140>  Not Yet Assigned
<141>  Concurrently Herewith

<150>  US 62/194,660
<151>  2015-07-20

<150>  US 62/152,723
<151>  2015-04-24

<160>  54    

<170>  PatentIn version 3.5

<210>  1
<211>  1019
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Arg Tyr Arg Leu Ala Trp Leu Leu His Pro Ala Leu Pro Ser Thr 
1               5                   10                  15      


Phe Arg Ser Val Leu Gly Ala Arg Leu Pro Pro Pro Glu Arg Leu Cys 
            20                  25                  30          


Gly Phe Gln Lys Lys Thr Tyr Ser Lys Met Asn Asn Pro Ala Ile Lys 
        35                  40                  45              


Arg Ile Gly Asn His Ile Thr Lys Ser Pro Glu Asp Lys Arg Glu Tyr 
    50                  55                  60                  


Arg Gly Leu Glu Leu Ala Asn Gly Ile Lys Val Leu Leu Ile Ser Asp 
65                  70                  75                  80  


Pro Thr Thr Asp Lys Ser Ser Ala Ala Leu Asp Val His Ile Gly Ser 
                85                  90                  95      


Leu Ser Asp Pro Pro Asn Ile Ala Gly Leu Ser His Phe Cys Glu His 
            100                 105                 110         


Met Leu Phe Leu Gly Thr Lys Lys Tyr Pro Lys Glu Asn Glu Tyr Ser 
        115                 120                 125             


Gln Phe Leu Ser Glu His Ala Gly Ser Ser Asn Ala Phe Thr Ser Gly 
    130                 135                 140                 


Glu His Thr Asn Tyr Tyr Phe Asp Val Ser His Glu His Leu Glu Gly 
145                 150                 155                 160 


Ala Leu Asp Arg Phe Ala Gln Phe Phe Leu Cys Pro Leu Phe Asp Glu 
                165                 170                 175     


Ser Cys Lys Asp Arg Glu Val Asn Ala Val Asp Ser Glu His Glu Lys 
            180                 185                 190         


Asn Val Met Asn Asp Ala Trp Arg Leu Phe Gln Leu Glu Lys Ala Thr 
        195                 200                 205             


Gly Asn Pro Lys His Pro Phe Ser Lys Phe Gly Thr Gly Asn Lys Tyr 
    210                 215                 220                 


Thr Leu Glu Thr Arg Pro Asn Gln Glu Gly Ile Asp Val Arg Gln Glu 
225                 230                 235                 240 


Leu Leu Lys Phe His Ser Ala Tyr Tyr Ser Ser Asn Leu Met Ala Val 
                245                 250                 255     


Cys Val Leu Gly Arg Glu Ser Leu Asp Asp Leu Thr Asn Leu Val Val 
            260                 265                 270         


Lys Leu Phe Ser Glu Val Glu Asn Lys Asn Val Pro Leu Pro Glu Phe 
        275                 280                 285             


Pro Glu His Pro Phe Gln Glu Glu His Leu Lys Gln Leu Tyr Lys Ile 
    290                 295                 300                 


Val Pro Ile Lys Asp Ile Arg Asn Leu Tyr Val Thr Phe Pro Ile Pro 
305                 310                 315                 320 


Asp Leu Gln Lys Tyr Tyr Lys Ser Asn Pro Gly His Tyr Leu Gly His 
                325                 330                 335     


Leu Ile Gly His Glu Gly Pro Gly Ser Leu Leu Ser Glu Leu Lys Ser 
            340                 345                 350         


Lys Gly Trp Val Asn Thr Leu Val Gly Gly Gln Lys Glu Gly Ala Arg 
        355                 360                 365             


Gly Phe Met Phe Phe Ile Ile Asn Val Asp Leu Thr Glu Glu Gly Leu 
    370                 375                 380                 


Leu His Val Glu Asp Ile Ile Leu His Met Phe Gln Tyr Ile Gln Lys 
385                 390                 395                 400 


Leu Arg Ala Glu Gly Pro Gln Glu Trp Val Phe Gln Glu Cys Lys Asp 
                405                 410                 415     


Leu Asn Ala Val Ala Phe Arg Phe Lys Asp Lys Glu Arg Pro Arg Gly 
            420                 425                 430         


Tyr Thr Ser Lys Ile Ala Gly Ile Leu His Tyr Tyr Pro Leu Glu Glu 
        435                 440                 445             


Val Leu Thr Ala Glu Tyr Leu Leu Glu Glu Phe Arg Pro Asp Leu Ile 
    450                 455                 460                 


Glu Met Val Leu Asp Lys Leu Arg Pro Glu Asn Val Arg Val Ala Ile 
465                 470                 475                 480 


Val Ser Lys Ser Phe Glu Gly Lys Thr Asp Arg Thr Glu Glu Trp Tyr 
                485                 490                 495     


Gly Thr Gln Tyr Lys Gln Glu Ala Ile Pro Asp Glu Val Ile Lys Lys 
            500                 505                 510         


Trp Gln Asn Ala Asp Leu Asn Gly Lys Phe Lys Leu Pro Thr Lys Asn 
        515                 520                 525             


Glu Phe Ile Pro Thr Asn Phe Glu Ile Leu Pro Leu Glu Lys Glu Ala 
    530                 535                 540                 


Thr Pro Tyr Pro Ala Leu Ile Lys Asp Thr Ala Met Ser Lys Leu Trp 
545                 550                 555                 560 


Phe Lys Gln Asp Asp Lys Phe Phe Leu Pro Lys Ala Cys Leu Asn Phe 
                565                 570                 575     


Glu Phe Phe Ser Pro Phe Ala Tyr Val Asp Pro Leu His Cys Asn Met 
            580                 585                 590         


Ala Tyr Leu Tyr Leu Glu Leu Leu Lys Asp Ser Leu Asn Glu Tyr Ala 
        595                 600                 605             


Tyr Ala Ala Glu Leu Ala Gly Leu Ser Tyr Asp Leu Gln Asn Thr Ile 
    610                 615                 620                 


Tyr Gly Met Tyr Leu Ser Val Lys Gly Tyr Asn Asp Lys Gln Pro Ile 
625                 630                 635                 640 


Leu Leu Lys Lys Ile Ile Glu Lys Met Ala Thr Phe Glu Ile Asp Glu 
                645                 650                 655     


Lys Arg Phe Glu Ile Ile Lys Glu Ala Tyr Met Arg Ser Leu Asn Asn 
            660                 665                 670         


Phe Arg Ala Glu Gln Pro His Gln His Ala Met Tyr Tyr Leu Arg Leu 
        675                 680                 685             


Leu Met Thr Glu Val Ala Trp Thr Lys Asp Glu Leu Lys Glu Ala Leu 
    690                 695                 700                 


Asp Asp Val Thr Leu Pro Arg Leu Lys Ala Phe Ile Pro Gln Leu Leu 
705                 710                 715                 720 


Ser Arg Leu His Ile Glu Ala Leu Leu His Gly Asn Ile Thr Lys Gln 
                725                 730                 735     


Ala Ala Leu Gly Ile Met Gln Met Val Glu Asp Thr Leu Ile Glu His 
            740                 745                 750         


Ala His Thr Lys Pro Leu Leu Pro Ser Gln Leu Val Arg Tyr Arg Glu 
        755                 760                 765             


Val Gln Leu Pro Asp Arg Gly Trp Phe Val Tyr Gln Gln Arg Asn Glu 
    770                 775                 780                 


Val His Asn Asn Cys Gly Ile Glu Ile Tyr Tyr Gln Thr Asp Met Gln 
785                 790                 795                 800 


Ser Thr Ser Glu Asn Met Phe Leu Glu Leu Phe Cys Gln Ile Ile Ser 
                805                 810                 815     


Glu Pro Cys Phe Asn Thr Leu Arg Thr Lys Glu Gln Leu Gly Tyr Ile 
            820                 825                 830         


Val Phe Ser Gly Pro Arg Arg Ala Asn Gly Ile Gln Gly Leu Arg Phe 
        835                 840                 845             


Ile Ile Gln Ser Glu Lys Pro Pro His Tyr Leu Glu Ser Arg Val Glu 
    850                 855                 860                 


Ala Phe Leu Ile Thr Met Glu Lys Ser Ile Glu Asp Met Thr Glu Glu 
865                 870                 875                 880 


Ala Phe Gln Lys His Ile Gln Ala Leu Ala Ile Arg Arg Leu Asp Lys 
                885                 890                 895     


Pro Lys Lys Leu Ser Ala Glu Cys Ala Lys Tyr Trp Gly Glu Ile Ile 
            900                 905                 910         


Ser Gln Gln Tyr Asn Phe Asp Arg Asp Asn Thr Glu Val Ala Tyr Leu 
        915                 920                 925             


Lys Thr Leu Thr Lys Glu Asp Ile Ile Lys Phe Tyr Lys Glu Met Leu 
    930                 935                 940                 


Ala Val Asp Ala Pro Arg Arg His Lys Val Ser Val His Val Leu Ala 
945                 950                 955                 960 


Arg Glu Met Asp Ser Cys Pro Val Val Gly Glu Phe Pro Cys Gln Asn 
                965                 970                 975     


Asp Ile Asn Leu Ser Gln Ala Pro Ala Leu Pro Gln Pro Glu Val Ile 
            980                 985                 990         


Gln Asn Met Thr Glu Phe Lys Arg  Gly Leu Pro Leu Phe  Pro Leu Val 
        995                 1000                 1005             


Lys Pro  His Ile Asn Phe Met  Ala Ala Lys Leu 
    1010                 1015                 


<210>  2
<211>  464
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Ser Lys Leu Trp Phe Lys Gln Asp Asp Lys Phe Phe Leu Pro Lys 
1               5                   10                  15      


Ala Cys Leu Asn Phe Glu Phe Phe Ser Pro Phe Ala Tyr Val Asp Pro 
            20                  25                  30          


Leu His Cys Asn Met Ala Tyr Leu Tyr Leu Glu Leu Leu Lys Asp Ser 
        35                  40                  45              


Leu Asn Glu Tyr Ala Tyr Ala Ala Glu Leu Ala Gly Leu Ser Tyr Asp 
    50                  55                  60                  


Leu Gln Asn Thr Ile Tyr Gly Met Tyr Leu Ser Val Lys Gly Tyr Asn 
65                  70                  75                  80  


Asp Lys Gln Pro Ile Leu Leu Lys Lys Ile Ile Glu Lys Met Ala Thr 
                85                  90                  95      


Phe Glu Ile Asp Glu Lys Arg Phe Glu Ile Ile Lys Glu Ala Tyr Met 
            100                 105                 110         


Arg Ser Leu Asn Asn Phe Arg Ala Glu Gln Pro His Gln His Ala Met 
        115                 120                 125             


Tyr Tyr Leu Arg Leu Leu Met Thr Glu Val Ala Trp Thr Lys Asp Glu 
    130                 135                 140                 


Leu Lys Glu Ala Leu Asp Asp Val Thr Leu Pro Arg Leu Lys Ala Phe 
145                 150                 155                 160 


Ile Pro Gln Leu Leu Ser Arg Leu His Ile Glu Ala Leu Leu His Gly 
                165                 170                 175     


Asn Ile Thr Lys Gln Ala Ala Leu Gly Ile Met Gln Met Val Glu Asp 
            180                 185                 190         


Thr Leu Ile Glu His Ala His Thr Lys Pro Leu Leu Pro Ser Gln Leu 
        195                 200                 205             


Val Arg Tyr Arg Glu Val Gln Leu Pro Asp Arg Gly Trp Phe Val Tyr 
    210                 215                 220                 


Gln Gln Arg Asn Glu Val His Asn Asn Cys Gly Ile Glu Ile Tyr Tyr 
225                 230                 235                 240 


Gln Thr Asp Met Gln Ser Thr Ser Glu Asn Met Phe Leu Glu Leu Phe 
                245                 250                 255     


Cys Gln Ile Ile Ser Glu Pro Cys Phe Asn Thr Leu Arg Thr Lys Glu 
            260                 265                 270         


Gln Leu Gly Tyr Ile Val Phe Ser Gly Pro Arg Arg Ala Asn Gly Ile 
        275                 280                 285             


Gln Gly Leu Arg Phe Ile Ile Gln Ser Glu Lys Pro Pro His Tyr Leu 
    290                 295                 300                 


Glu Ser Arg Val Glu Ala Phe Leu Ile Thr Met Glu Lys Ser Ile Glu 
305                 310                 315                 320 


Asp Met Thr Glu Glu Ala Phe Gln Lys His Ile Gln Ala Leu Ala Ile 
                325                 330                 335     


Arg Arg Leu Asp Lys Pro Lys Lys Leu Ser Ala Glu Cys Ala Lys Tyr 
            340                 345                 350         


Trp Gly Glu Ile Ile Ser Gln Gln Tyr Asn Phe Asp Arg Asp Asn Thr 
        355                 360                 365             


Glu Val Ala Tyr Leu Lys Thr Leu Thr Lys Glu Asp Ile Ile Lys Phe 
    370                 375                 380                 


Tyr Lys Glu Met Leu Ala Val Asp Ala Pro Arg Arg His Lys Val Ser 
385                 390                 395                 400 


Val His Val Leu Ala Arg Glu Met Asp Ser Cys Pro Val Val Gly Glu 
                405                 410                 415     


Phe Pro Cys Gln Asn Asp Ile Asn Leu Ser Gln Ala Pro Ala Leu Pro 
            420                 425                 430         


Gln Pro Glu Val Ile Gln Asn Met Thr Glu Phe Lys Arg Gly Leu Pro 
        435                 440                 445             


Leu Phe Pro Leu Val Lys Pro His Ile Asn Phe Met Ala Ala Lys Leu 
    450                 455                 460                 


<210>  3
<211>  1019
<212>  PRT
<213>  Mus musculus

<400>  3

Met Arg Asn Gly Leu Val Trp Leu Leu His Pro Ala Leu Pro Gly Thr 
1               5                   10                  15      


Leu Arg Ser Ile Leu Gly Ala Arg Pro Pro Pro Ala Lys Arg Leu Cys 
            20                  25                  30          


Gly Phe Pro Lys Gln Thr Tyr Ser Thr Met Ser Asn Pro Ala Ile Gln 
        35                  40                  45              


Arg Ile Glu Asp Gln Ile Val Lys Ser Pro Glu Asp Lys Arg Glu Tyr 
    50                  55                  60                  


Arg Gly Leu Glu Leu Ala Asn Gly Ile Lys Val Leu Leu Ile Ser Asp 
65                  70                  75                  80  


Pro Thr Thr Asp Lys Ser Ser Ala Ala Leu Asp Val His Ile Gly Ser 
                85                  90                  95      


Leu Ser Asp Pro Pro Asn Ile Pro Gly Leu Ser His Phe Cys Glu His 
            100                 105                 110         


Met Leu Phe Leu Gly Thr Lys Lys Tyr Pro Lys Glu Asn Glu Tyr Ser 
        115                 120                 125             


Gln Phe Leu Ser Glu His Ala Gly Ser Ser Asn Ala Phe Thr Ser Gly 
    130                 135                 140                 


Glu His Thr Asn Tyr Tyr Phe Asp Val Ser His Glu His Leu Glu Gly 
145                 150                 155                 160 


Ala Leu Asp Arg Phe Ala Gln Phe Phe Leu Cys Pro Leu Phe Asp Ala 
                165                 170                 175     


Ser Cys Lys Asp Arg Glu Val Asn Ala Val Asp Ser Glu His Glu Lys 
            180                 185                 190         


Asn Val Met Asn Asp Ala Trp Arg Leu Phe Gln Leu Glu Lys Ala Thr 
        195                 200                 205             


Gly Asn Pro Lys His Pro Phe Ser Lys Phe Gly Thr Gly Asn Lys Tyr 
    210                 215                 220                 


Thr Leu Glu Thr Arg Pro Asn Gln Glu Gly Ile Asp Val Arg Glu Glu 
225                 230                 235                 240 


Leu Leu Lys Phe His Ser Thr Tyr Tyr Ser Ser Asn Leu Met Ala Ile 
                245                 250                 255     


Cys Val Leu Gly Arg Glu Ser Leu Asp Asp Leu Thr Asn Leu Val Val 
            260                 265                 270         


Lys Leu Phe Ser Glu Val Glu Asn Lys Asn Val Pro Leu Pro Glu Phe 
        275                 280                 285             


Pro Glu His Pro Phe Gln Glu Glu His Leu Arg Gln Leu Tyr Lys Ile 
    290                 295                 300                 


Val Pro Ile Lys Asp Ile Arg Asn Leu Tyr Val Thr Phe Pro Ile Pro 
305                 310                 315                 320 


Asp Leu Gln Gln Tyr Tyr Lys Ser Asn Pro Gly His Tyr Leu Gly His 
                325                 330                 335     


Leu Ile Gly His Glu Gly Pro Gly Ser Leu Leu Ser Glu Leu Lys Ser 
            340                 345                 350         


Lys Gly Trp Val Asn Thr Leu Val Gly Gly Gln Lys Glu Gly Ala Arg 
        355                 360                 365             


Gly Phe Met Phe Phe Ile Ile Asn Val Asp Leu Thr Glu Glu Gly Leu 
    370                 375                 380                 


Leu His Val Glu Asp Ile Ile Leu His Met Phe Gln Tyr Ile Gln Lys 
385                 390                 395                 400 


Leu Arg Ala Glu Gly Pro Gln Glu Trp Val Phe Gln Glu Cys Lys Asp 
                405                 410                 415     


Leu Asn Ala Val Ala Phe Arg Phe Lys Asp Lys Glu Arg Pro Arg Gly 
            420                 425                 430         


Tyr Thr Ser Lys Ile Ala Gly Lys Leu His Tyr Tyr Pro Leu Asn Gly 
        435                 440                 445             


Val Leu Thr Ala Glu Tyr Leu Leu Glu Glu Phe Arg Pro Asp Leu Ile 
    450                 455                 460                 


Asp Met Val Leu Asp Lys Leu Arg Pro Glu Asn Val Arg Val Ala Ile 
465                 470                 475                 480 


Val Ser Lys Ser Phe Glu Gly Lys Thr Asp Arg Thr Glu Gln Trp Tyr 
                485                 490                 495     


Gly Thr Gln Tyr Lys Gln Glu Ala Ile Pro Glu Asp Ile Ile Gln Lys 
            500                 505                 510         


Trp Gln Asn Ala Asp Leu Asn Gly Lys Phe Lys Leu Pro Thr Lys Asn 
        515                 520                 525             


Glu Phe Ile Pro Thr Asn Phe Glu Ile Leu Ser Leu Glu Lys Asp Ala 
    530                 535                 540                 


Thr Pro Tyr Pro Ala Leu Ile Lys Asp Thr Ala Met Ser Lys Leu Trp 
545                 550                 555                 560 


Phe Lys Gln Asp Asp Lys Phe Phe Leu Pro Lys Ala Cys Leu Asn Phe 
                565                 570                 575     


Glu Phe Phe Ser Pro Phe Ala Tyr Val Asp Pro Leu His Cys Asn Met 
            580                 585                 590         


Ala Tyr Leu Tyr Leu Glu Leu Leu Lys Asp Ser Leu Asn Glu Tyr Ala 
        595                 600                 605             


Tyr Ala Ala Glu Leu Ala Gly Leu Ser Tyr Asp Leu Gln Asn Thr Ile 
    610                 615                 620                 


Tyr Gly Met Tyr Leu Ser Val Lys Gly Tyr Asn Asp Lys Gln Pro Ile 
625                 630                 635                 640 


Leu Leu Lys Lys Ile Thr Glu Lys Met Ala Thr Phe Glu Ile Asp Lys 
                645                 650                 655     


Lys Arg Phe Glu Ile Ile Lys Glu Ala Tyr Met Arg Ser Leu Asn Asn 
            660                 665                 670         


Phe Arg Ala Glu Gln Pro His Gln His Ala Met Tyr Tyr Leu Arg Leu 
        675                 680                 685             


Leu Met Thr Glu Val Ala Trp Thr Lys Asp Glu Leu Lys Glu Ala Leu 
    690                 695                 700                 


Asp Asp Val Thr Leu Pro Arg Leu Lys Ala Phe Ile Pro Gln Leu Leu 
705                 710                 715                 720 


Ser Arg Leu His Ile Glu Ala Leu Leu His Gly Asn Ile Thr Lys Gln 
                725                 730                 735     


Ala Ala Leu Gly Val Met Gln Met Val Glu Asp Thr Leu Ile Glu His 
            740                 745                 750         


Ala His Thr Lys Pro Leu Leu Pro Ser Gln Leu Val Arg Tyr Arg Glu 
        755                 760                 765             


Val Gln Leu Pro Asp Arg Gly Trp Phe Val Tyr Gln Gln Arg Asn Glu 
    770                 775                 780                 


Val His Asn Asn Cys Gly Ile Glu Ile Tyr Tyr Gln Thr Asp Met Gln 
785                 790                 795                 800 


Ser Thr Ser Glu Asn Met Phe Leu Glu Leu Phe Cys Gln Ile Ile Ser 
                805                 810                 815     


Glu Pro Cys Phe Asn Thr Leu Arg Thr Lys Glu Gln Leu Gly Tyr Ile 
            820                 825                 830         


Val Phe Ser Gly Pro Arg Arg Ala Asn Gly Ile Gln Gly Leu Arg Phe 
        835                 840                 845             


Ile Ile Gln Ser Glu Lys Pro Pro His Tyr Leu Glu Ser Arg Val Glu 
    850                 855                 860                 


Ala Phe Leu Ile Thr Met Glu Lys Ala Ile Glu Asp Met Thr Glu Glu 
865                 870                 875                 880 


Ala Phe Gln Lys His Ile Gln Ala Leu Ala Ile Arg Arg Leu Asp Lys 
                885                 890                 895     


Pro Lys Lys Leu Ser Ala Glu Cys Ala Lys Tyr Trp Gly Glu Ile Ile 
            900                 905                 910         


Ser Gln Gln Tyr Asn Tyr Asp Arg Asp Asn Ile Glu Val Ala Tyr Leu 
        915                 920                 925             


Lys Thr Leu Thr Lys Asp Asp Ile Ile Arg Phe Tyr Gln Glu Met Leu 
    930                 935                 940                 


Ala Val Asp Ala Pro Arg Arg His Lys Val Ser Val His Val Leu Ala 
945                 950                 955                 960 


Arg Glu Met Asp Ser Cys Pro Val Val Gly Glu Phe Pro Ser Gln Asn 
                965                 970                 975     


Asp Ile Asn Leu Ser Glu Ala Pro Pro Leu Pro Gln Pro Glu Val Ile 
            980                 985                 990         


His Asn Met Thr Glu Phe Lys Arg  Gly Leu Pro Leu Phe  Pro Leu Val 
        995                 1000                 1005             


Lys Pro  His Ile Asn Phe Met  Ala Ala Lys Leu 
    1010                 1015                 


<210>  4
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Arg is modified with (7-methoxycoumarin-4-yl)acetyl

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Lys is modified with 2,4-dinitrophenyl

<400>  4

Arg Pro Pro Gly Phe Ser Ala Phe Lys 
1               5                   


<210>  5
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  5

Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Asn 
            20      


<210>  6
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  6

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr 
            20                  25                  30  


<210>  7
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  7

His Ser Gln Gly Thr Phe Thr Ser Asp Tyr Ser Lys Tyr Leu Asp Ser 
1               5                   10                  15      


Arg Arg Ala Gln Asp Phe Val Gln Trp Leu Met Asn Thr 
            20                  25                  


<210>  8
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa is Lys-(gamma)N-anthrinilamide

<220>
<221>  MISC_FEATURE
<222>  (23)..(23)
<223>  Xaa is 3-nitro-Tyr

<400>  8

Lys Cys Asn Thr Ala Thr Cys Ala Thr Xaa Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Asn Asn Xaa Gly Ala Ile Leu Ser Ser Thr Asn Val 
            20                  25                  30          


Gly Ser Asn Thr Tyr 
        35          


<210>  9
<211>  37
<212>  PRT
<213>  Homo sapiens

<400>  9

Lys Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Asn Asn Phe Gly Ala Ile Leu Ser Ser Thr Asn Val 
            20                  25                  30          


Gly Ser Asn Thr Tyr 
        35          


<210>  10
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Arg is modified with (7-methoxycoumarin-4-yl)acetyl

<220>
<221> MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa is norvaline

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Lys is modified with 2,4-dinitrophenyl

<400>  10

Arg Pro Lys Pro Val Glu Xaa Trp Arg Lys 
1               5                   10  


<210>  11
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Pro is modified with (7-methoxycoumarin-4-yl)acetyl

<220>
<221>  MISC_FEATURE
<222>  (6)..(7)
<223>  Modified with N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl

<400>  11

Pro Leu Ala Gln Ala Val Arg Ser Ser Ser Arg 
1               5                   10      


<210>  12
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Ser is modified with (7-methoxycoumarin-4-yl)acetyl

<220>
<221>  MISC_FEATURE
<222>  (11)..(12)
<223>  Modified with 2,4-dinitrophenyl

<400>  12

Ser Glu Val Asn Leu Asp Ala Glu Phe Arg Lys Arg Arg 
1               5                   10              


<210>  13
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Pro is modified with (7-methoxycoumarin-4-yl)acetyl

<220>
<221>  MISC_FEATURE
<222>  (4)..(5)
<223>  Modified with N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl

<400>  13

Pro Leu Gly Leu Ala Arg 
1               5       


<210>  14
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Tyr is modified with PO3

<400>  14

Asp Ala Asp Glu Tyr Leu Ile Pro Gln Gln Gly 
1               5                   10      


<210>  15
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Tyr is modified with (7-methoxycoumarin-4-yl)acetyl

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Lys is modified with 2,4-dinitrophenyl

<400>  15

Tyr Val Ala Asp Ala Pro Lys 
1               5           


<210>  16
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Ala is acetylated

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Asp is modified with 7-amino-4-methylcoumarin

<400>  16

Ala Ser Thr Asp 
1               


<210>  17
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Ala is modified with benzyloxycarbonyl

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Asp is modified with 7-amino-4-trifluoromethylcoumarin

<400>  17

Asp Gln Met Asp 
1               


<210>  18
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Met is modified with p-nitroanilide

<400>  18

Lys Thr Glu Glu Ile Ser Glu Val Lys Met 
1               5                   10  


<210>  19
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Ala is modified with succinyl

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is norvaline

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is modified with p-nitroanilide

<400>  19

Ala Ala Pro Xaa 
1               


<210>  20
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Lys is modified with 2-aminobenzoic acid

<220>
<221>  MISC_FEATURE
<222>  (5)..(6)
<223>  Modified with L-alpha,beta-diaminopropionic acid and 2,4-dinitrophenyl

<400>  20

Lys Pro Leu Gly Leu Ala Arg 
1               5           


<210>  21
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Tyr is modified with 4-(4-dimethylaminophenylazo)benzoyl

<400>  21

Tyr Val Ala Asp 
1               


<210>  22
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(2)
<223>  Modified with 5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Lys is modified with 4-(4-dimethylaminophenylazo)benzoyl

<400>  22

Glu Lys Pro Ala Lys Phe Phe Arg Lys 
1               5                   


<210>  23
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Gly is modified with 5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  Xaa is retro-m-nitro-Tyr-H


<400>  23

Gly Gly Trp Thr Leu Asn Ser Ala Gly Tyr Leu Lys Xaa 
1               5                   10          


<210>  24
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Arg is modified with (7 methoxycourmarin-4-yl)acetyl

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Lys is modified with 2,4-dinitrophenyl

<400>  24

Arg Pro Pro Gly Phe Ser Ala Phe Lys 
1               5                   


<210>  25
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Pro is modified with (7 methoxycourmarin-4-yl)acetyl

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Lys is modified with 2,4-dinitrophenyl

<400>  25

Pro Leu Gly Pro Lys 
1               5   


<210>  26
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  26
atcatcatat gaataatcca gccaucaaga gaatagg                                37


<210>  27
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  27
atgctagcca tacctcagag utttgcagcc atgaag                                 36


<210>  28
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  28
atggctggat tattcatatg atgaugatga tgatgagaac cc                          42


<210>  29
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  29
actctgaggt atggctagca ugactggtg                                         29


<210>  30
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  30
agactctttc aattggaaaa agcuacaggg                                        30


<210>  31
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  31
agctttttcc aattgaaaga gtcuccaggt atcattcatc acattcttct catgttc          57


<210>  32
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  32
agactctttc aattggaaaa agcuacaggg                                        30


<210>  33
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  33
agctttttcc aattgaaaga gtcugaaggc atcattcatc acattcttct c                51


<210>  34
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  34
agactctttc aattggaaaa agcuacaggg                                        30


<210>  35
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  35
atgaatgatg cctggagacu ccgtcaattg gaaaaagcta caggg                       45


<210>  36
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  36
acccattaaa gatattagga atctcutcgt gacatttccc atacctgacc ttc              53


<210>  37
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  37
agagattcct aatatcttta atggguacta ttttg                                  35


<210>  38
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  38
aaagggctgg gttaatactc tucagggtgg gcag                                   34


<210>  39
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  39
aagagtatta acccagccct tugacttaag                                        30


<210>  40
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  40
aaagggctgg gttaatactc tugttggtca gcagaaggaa ggagcccgag                  50


<210>  41
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  41
aagagtatta acccagccct tugacttaag                                        30


<210>  42
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  42
atgttttttc agattaatgt ggactugacc gaggaagg                               38


<210>  43
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  43
aagtccacat taatctgaaa aaacauaaaa cctcgggctc cttc                        44


<210>  44
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  44
atgtccgggt tctgatagtt tctaaauctt ttgaaggaaa aactg                       45


<210>  45
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  45
atttagaaac tatcagaacc cggacauttt ctggtctgag                             40


<210>  46
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  46
gattaacttt attattaaaa attaaagagg                                        30


<210>  47
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  47
caacatgtaa taatccttcc tcggtc                                            26


<210>  48
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  48
gcatgaaggt cctggaagtc tg                                                22


<210>  49
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  49
aggaagggtt acatcatcca gagc                                              24


<210>  50
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  50
ccatgtacta cctccgcttg c                                                 21


<210>  51
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  51
gcagatctcg agctcggatc                                                   20


<210>  52
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  52
gcttatgtgg accccttgca ctg                                               23


<210>  53
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  53

Arg Ala Gln Asp Phe Val Gln Trp Leu Met Asn Thr 
1               5                   10          


<210>  54
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  54

Arg Arg Ala Gln Asp Phe Val Gln Trp Leu Met Asn Thr 
1               5                   10              


