                         SEQUENCE LISTING

<110>  National University of Singapore
 
<120>  Targeting Telomerase for Cell Therapy

<130>  FP8043

<160>  19    

<170>  PatentIn version 3.5

<210>  1
<211>  34
<212>  DNA
<213>  Artificial sequence

<220>
<223>  FRT recognition site sequence

<400>  1
gaagttccta ttctctagaa agtataggaa cttc                                   34


<210>  2
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LoxP recognition site


<220>
<221>  misc_feature
<222>  (14)..(21)
<223>  "n" indicates bases which may vary

<400>  2
ataacttcgt atannntann ntatacgaag ttat                                   34


<210>  3
<211>  32
<212>  DNA
<213>  Artificial sequence

<220>
<223>  hTERT exon1 primer with restriction site

<400>  3
ttttgtcgac tgctgcgcac gtgggaagcc ct                                     32


<210>  4
<211>  33
<212>  DNA
<213>  Artificial sequence

<220>
<223>  hTERT intron2 primer with restriction site

<400>  4
ttttggatcc atgcaaactg gacaggaggg aga                                    33


<210>  5
<211>  66
<212>  DNA
<213>  Artificial sequence

<220>
<223>  LoxP site oligonucleotide

<400>  5
caggcagcgc tgcgtcataa cttcgtataa tgtatgctat acgaagttat ctgctgcgca       60

cgtggg                                                                  66


<210>  6
<211>  66
<212>  DNA
<213>  Artificial sequence

<220>
<223>  LoxP site oligonucleotide

<400>  6
cccacgtgcg cagcagataa cttcgtatag catacattat acgaagttat gacgcagcgc       60

tgcctg                                                                  66


<210>  7
<211>  35
<212>  DNA
<213>  Artificial sequence

<220>
<223>  to amplify 3' homologue arm of the targeting vector

<400>  7
aaaagctagc ttacgaggtt caccttcacg ttttg                                  35


<210>  8
<211>  34
<212>  DNA
<213>  Artificial sequence

<220>
<223>  to amplify 3' homologue arm of targeting vector

<400>  8
aaaactcgag aacagcaatg acaggcagag tcct                                   34


<210>  9
<211>  53
<212>  DNA
<213>  Artificial sequence

<220>
<223>  to eliminate Sac I site in PGK fragments

<400>  9
gagcagtggt ggaatgcaga tcctagcagc tcgctgatca gcctcgactg tgc              53


<210>  10
<211>  43
<212>  DNA
<213>  Artificial sequence

<220>
<223>  to eliminate Sac I site in PGK fragments

<400>  10
ctgaggggat caattctcta gcagctcgct gatcagcctc gac                         43


<210>  11
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  hTERT upstream primer

<400>  11
aagggaacaa aagctggagc tcg                                               23


<210>  12
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  hTERT upstream primer

<400>  12
cagtagaagc cacacggcca ctg                                               23


<210>  13
<211>  57
<212>  DNA
<213>  Artificial sequence

<220>
<223>  to eliminate the ZFN binding sites on the targeting vector

<400>  13
gagccgtgcg ctccctgctg agatctcact acagagaggt gctgccgctg gccacgt          57


<210>  14
<211>  57
<212>  DNA
<213>  Artificial sequence

<220>
<223>  to eliminate the ZFN binding sites on the targeting vector

<400>  14
acgtggccag cggcagcacc tctctgtagt gagatctcag cagggagcgc acggctc          57


<210>  15
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRISPR guide RNA S1 to target hTERT exon 1

<400>  15
gggggcggcc gtgcgtccca ggg                                               23


<210>  16
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRISPR guide RNA S2 to target hTERT exon 1

<400>  16
gctgcgcagc cactaccgcg agg                                               23


<210>  17
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRISPR guide RNA S3 to target hTERT exon 1

<400>  17
gcagcaggga gcgcacggct cgg                                               23


<210>  18
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRISPR guide RNA S4 to target hTERT exon 1

<400>  18
ggggagcgcg cggcatcgcg ggg                                               23


<210>  19
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRISPR guide RNA S5 to target hTERT exon 1

<400>  19
gggagcgcgc ggcatcgcgg ggg                                               23


