                         SEQUENCE LISTING

<110>  Biosearch Technologies, Inc.
       Cook, Ronald M.
       Sowers, Ben
       Beal, Marc P.
 
<120>  DUAL QUENCHER PROBES

<130>  061873-5034-WO

<140>  PCT/US2016/027964
<141>  2016-04-15

<150>  US 62/147,695
<151>  2015-04-15

<160>  19    

<170>  PatentIn version 3.5

<210>  1
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  21-base probe (end-labeled)


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Labeled with FAM

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  Labeled with BHQ1

<400>  1
tcctttgggc tcctgccatc t                                                 21


<210>  2
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  21-base probe (I-BHQ0 + 3-BHQ1)


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Labeled with FAM

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  Residues No. 9 and No. 10 are linked through a BHQ0 modification

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  Labeled with BHQ1

<400>  2
tcctttgggc tcctgccatc t                                                 21


<210>  3
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  29-base probe (end-labeled)


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Labeled with FAM

<220>
<221>  misc_feature
<222>  (29)..(29)
<223>  Labeled with BHQ1

<400>  3
aaaccacttt atgaaaatct aactggaca                                         29


<210>  4
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  29-base probe (I-BHQ0 + 3-BHQ1)


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Labeled with FAM

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  Residues No. 9 and No. 10 are linked through a BHQ0 modification

<220>
<221>  misc_feature
<222>  (29)..(29)
<223>  Labeled with BHQ1

<400>  4
aaaccacttt atgaaaatct aactggaca                                         29


<210>  5
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  35-base probe (end-labeled)


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Labeled with FAM

<220>
<221>  misc_feature
<222>  (35)..(35)
<223>  Labeled with BHQ1

<400>  5
aagagtagta gcctaagagt gtcagttgta catca                                  35


<210>  6
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  35-base probe (I-BHQ0 + 3-BHQ1)


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Labeled with FAM

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  Residues No. 9 and No. 10 are linked through a BHQ0 modification

<220>
<221>  misc_feature
<222>  (35)..(35)
<223>  Labeled with BHQ1

<400>  6
aagagtagta gcctaagagt gtcagttgta catca                                  35


<210>  7
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  21-base probe (I-TBHQ1 + 3-Sp3)


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Labeled with FAM

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Modified with BHQ1

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  Modified with Spacer-C3

<400>  7
tcctttgggc tcctgccatc t                                                 21


<210>  8
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  21-base probe (I-TBHQ1 + 3-BHQ1)


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Labeled with FAM

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Modified with BHQ1

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  Labeled with BHQ1

<400>  8
tcctttgggc tcctgccatc t                                                 21


<210>  9
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  21-base probe (I-BHQ1 + 3-BHQ1)


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Labled with FAM

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  Residues No. 9 and No. 10 are linked through a BHQ1 modification

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  Labled with BHQ1

<400>  9
tcctttgggc tcctgccatc t                                                 21


<210>  10
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  35-base probe (I-TDAB + 3-BHQ1)


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Labeled with FAM

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  Modified with DAB moiety

<220>
<221>  misc_feature
<222>  (35)..(35)
<223>  Labeled with BHQ1

<400>  10
aagagtagta gcctaagagt gtcagttgta catca                                  35


<210>  11
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  35-base probe (I-Aniline + 3-BHQ1)


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Labeled with FAM

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  Residues No. 9 and No. 10 are linked through an aniline moiety

<220>
<221>  misc_feature
<222>  (35)..(35)
<223>  Labeled with BHQ1

<400>  11
aagagtagta gcctaagagt gtcagttgta catca                                  35


<210>  12
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  35-base probe (I-dRBHQ0 + 3-BHQ1)


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Labeled with FAM

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  Residues No. 9 and No. 10 are linked through a dRBHQ0 moiety

<220>
<221>  misc_feature
<222>  (35)..(35)
<223>  Labeled with BHQ1

<400>  12
aagagtagta gcctaagagt gtcagttgta catca                                  35


<210>  13
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  35-base probe (I-gBHQ0 + 3-BHQ1)


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Labeled with FAM

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  Residues No. 9 and No. 10 are linked through a gBHQ0 moiety

<220>
<221>  misc_feature
<222>  (35)..(35)
<223>  Labeled with BHQ1

<400>  13
aagagtagta gcctaagagt gtcagttgta catca                                  35


<210>  14
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  35-base probe (I-BHQ0 + 3-Sp3)


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Labeled with FAM

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  Residues No. 9 and No. 10 are linked through a BHQ0 modification

<220>
<221>  misc_feature
<222>  (35)..(35)
<223>  Modified with Spacer-C3

<400>  14
aagagtagta gcctaagagt gtcagttgta catca                                  35


<210>  15
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  35-base probe (I-BHQ1 + 3-BHQ0)


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Labeled with FAM

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  Residues No. 9 and No. 10 are linked through a BHQ1 modification

<220>
<221>  misc_feature
<222>  (35)..(35)
<223>  Labeled with BHQ0

<400>  15
aagagtagta gcctaagagt gtcagttgta catca                                  35


<210>  16
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  6/7 I-BHQ0 + 3-BHQ1 probe


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Labeled with FAM

<220>
<221>  misc_feature
<222>  (6)..(7)
<223>  Residues No. 6 and No. 7 are linked through a BHQ0 modification

<220>
<221>  misc_feature
<222>  (35)..(35)
<223>  Labeled with BHQ1

<400>  16
aagagtagta gcctaagagt gtcagttgta catca                                  35


<210>  17
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  7/8 I-BHQ0 + 3-BHQ1 probe


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Labeled with FAM

<220>
<221>  misc_feature
<222>  (7)..(8)
<223>  Residues No. 7 and No. 8 are linked through a BHQ0 modification

<220>
<221>  misc_feature
<222>  (35)..(35)
<223>  Labeled with BHQ1

<400>  17
aagagtagta gcctaagagt gtcagttgta catca                                  35


<210>  18
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  8/9 I-BHQ0 + 3-BHQ1 probe


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Labeled with FAM

<220>
<221>  misc_feature
<222>  (8)..(9)
<223>  Residues No. 8 and No. 9 are linked through a BHQ0 modification

<220>
<221>  misc_feature
<222>  (35)..(35)
<223>  Labeled with BHQ1

<400>  18
aagagtagta gcctaagagt gtcagttgta catca                                  35


<210>  19
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  11/12 I-BHQ0 + 3-BHQ1


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Labeled with FAM

<220>
<221>  misc_feature
<222>  (11)..(12)
<223>  Residues No. 11 and No. 12 are linked through a BHQ0 modification

<220>
<221>  misc_feature
<222>  (35)..(35)
<223>  Labled with BHQ1

<400>  19
aagagtagta gcctaagagt gtcagttgta catca                                  35


