                         SEQUENCE LISTING

<110>  President and Fellows of Harvard College
 
<120>  PRODUCTION AND MONITORING OF METABOLITES IN CELLS

<130>  010498.00554

<140>  62/146,478
<141>  2015-04-13

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  Binding site

<400>  1
tttaacttta agaaggagat atacat                                            26


<210>  2
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Promoter

<400>  2
gaaataagga ggtaatacaa                                                   20


<210>  3
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  Promoter

<400>  3
gcttcacaac cgcacttgat ttaatagacc ataccgtcta ttatttctgg                  50


<210>  4
<211>  320
<212>  DNA
<213>  Artificial

<220>
<223>  Promoter

<400>  4
agaaaccaat tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt actggctctt       60

ctcgctaacc caaccggtaa ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa      120

agccatgaca aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag tccacattga      180

ttatttgcac ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga      240

tcctacctga cgctttttat cgcaactctc tactgtttct ccatacccgc tttcatatct      300

ttcacttttt ttgggctaac                                                  320


<210>  5
<211>  521
<212>  DNA
<213>  Artificial

<220>
<223>  Promoter

<400>  5
atgctgttga ttgacgccag tgagaacccg gaaccggaaa cggaatcaaa tccgtgggtc       60

gaacagtggg gcacgctgtt gtcctgatat gttcagcgag cggtaaatgt cgttttagcg      120

gtgctgaatc gaatcttttc aggcaaatgc cagtaaaaac tgcttcatag cgcggatttt      180

tactggcgtt tgcctggagt caagcgatcc atttcatact cttctttatt tcttcgtttt      240

aacccttcct ttcttgttct tgttttcatt tccgtgaagt ggattccacc gtccagggct      300

aatgccaaaa tcgggcctca ttgaacgcat taatgttgtg ttgttgcacg gtgagccgct      360

atggcgcgct ttttatactg ctattgccag atataaacac gcgccgtatt cggcgaacga      420

cctataaaaa cggcaaaaaa caccctacgt cacctctgat ttcctggcga tgtcgcagtc      480

cagagtgagc gtggctaacg cgaattttca ggagtgcaac a                          521


<210>  6
<211>  52
<212>  DNA
<213>  Artificial

<220>
<223>  Promoter

<400>  6
ggattgaata taaccgacgt gactgttaca tttaggtggc taaacccgtc aa               52


<210>  7
<211>  91
<212>  DNA
<213>  Artificial

<220>
<223>  Promoter

<400>  7
tcgagtccct atcagtgata gagattgaca tccctatcag tgatagagat actgagcaca       60

tcagcaggac gcactgaccg aattcattaa a                                      91


<210>  8
<211>  51
<212>  DNA
<213>  Artificial

<220>
<223>  Promoter

<400>  8
cacccagcag tatttacaaa caaccatgaa tgtaagtata ttccttagca a                51


<210>  9
<211>  660
<212>  DNA
<213>  Artificial

<220>
<223>  Regulator

<400>  9
atgccgctga ccgacacccc gccgtctgtt ccgcagaaac cgcgtcgtgg tcgtccgcgt       60

ggtgctccgg acgcttctct ggctcaccag tctctgatcc gtgctggtct ggaacacctg      120

accgaaaaag gttactcttc tgttggtgtt gacgaaatcc tgaaagctgc tcgtgttccg      180

aaaggttctt tctaccacta cttccgtaac aaagctgact tcggtctggc tctgatcgaa      240

gcttacgaca cctacttcgc tcgtctcctc gaccaggcgt tcctggacgg ttcgctggct      300

ccgctggctc gtctgcgtct gttcacccgt atggctgaag aaggtatggc tcgtcacggt      360

ttccgtcgtg gttgcctggt tggtaacctg ggtcaggaaa tgggtgctct gccggacgac      420

ttccgtgctg ctctgatcgg tgttctggaa acctggcagc gtcgtaccgc tcagctgttc      480

cgtgaagctc aggcttgcgg tgaactgtct gctgaccacg acccggacgc tctggctgaa      540

gctttctgga tcggttggga aggtgctatc ctgcgtgcta aactggaact gcgtccggac      600

ccgctgcact ctttcacccg taccttcggt cgtcacttcg ttacccgtac ccaggaataa      660


<210>  10
<211>  879
<212>  DNA
<213>  Artificial

<220>
<223>  Regulator

<400>  10
atggctgaag cgcaaaatga tcccctgctg ccgggatact cgtttaacgc ccatctggtg       60

gcgggtttaa cgccgattga ggccaacggt tatctcgatt tttttatcga ccgaccgctg      120

ggaatgaaag gttatattct caatctcacc attcgcggtc agggggtggt gaaaaatcag      180

ggacgagaat ttgtctgccg accgggtgat attttgctgt tcccgccagg agagattcat      240

cactacggtc gtcatccgga ggctcgcgaa tggtatcacc agtgggttta ctttcgtccg      300

cgcgcctact ggcatgaatg gcttaactgg ccgtcaatat ttgccaatac gggtttcttt      360

cgcccggatg aagcgcacca gccgcatttc agcgacctgt ttgggcaaat cattaacgcc      420

gggcaagggg aagggcgcta ttcggagctg ctggcgataa atctgcttga gcaattgtta      480

ctgcggcgca tggaagcgat taacgagtcg ctccatccac cgatggataa tcgggtacgc      540

gaggcttgtc agtacatcag cgatcacctg gcagacagca attttgatat cgccagcgtc      600

gcacagcatg tttgcttgtc gccgtcgcgt ctgtcacatc ttttccgcca gcagttaggg      660

attagcgtct taagctggcg cgaggaccaa cgcattagtc aggcgaagct gcttttgagc      720

actacccgga tgcctatcgc caccgtcggt cgcaatgttg gttttgacga tcaactctat      780

ttctcgcgag tatttaaaaa atgcaccggg gccagcccga gcgagtttcg tgccggttgt      840

gaagaaaaag tgaatgatgt agccgtcaag ttgtcataa                             879


<210>  11
<211>  1158
<212>  DNA
<213>  Artificial

<220>
<223>  Regulator

<400>  11
atggctggct ggcatcttga taccaaaatg gcgcaggata tcgtggcacg taccatgcgc       60

atcatcgata ccaatatcaa cgtaatggat gcccgtgggc gaattatcgg cagcggcgat      120

cgtgagcgta ttggtgaatt gcacgaaggt gcattgctgg tactttcaca gggacgagtc      180

gtcgatatcg atgacgcggt agcacgtcat ctgcacggtg tgcggcaggg gattaatcta      240

ccgttacggc tggaaggtga aattgtcggc gtaattggcc tgacaggtga accagagaat      300

ctgcgtaaat atggcgaact ggtctgcatg acggctgaaa tgatgctgga acagtcgcgg      360

ttgatgcact tgttggcgca ggatagccgt ttgcgggaag aactggtgat gaacctgatt      420

caggcagagg agaatactcc cgcacttact gaatgggcgc aacggctggg gatcgatctc      480

aatcaaccgc gagtggtggc tattgttgag gtcgacagcg gtcagcttgg cgtggacagc      540

gcaatggcgg agttacaaca actgcaaaac gcgctgacta cgcccgagcg taataatctg      600

gtggcgattg tctcgctaac cgaaatggtg gtgttgaaac cggcgttgaa ctcttttggg      660

cgctgggatg cagaagatca tcgtaagcga gttgaacaac tgattacccg catgaaagag      720

tacggccagc tgcgttttcg cgtttcactg ggcaactatt ttaccggtcc tggcagtatt      780

gcccgatcct atcgtacggc gaaaacgacg atggtggtgg gtaaacagcg gatgccagaa      840

agtcgctgct atttttatca ggatctgatg ttacctgtgt tactcgacag tttgcgtggc      900

gactggcagg ccaacgaact ggcgcgaccg ctggcgcggc tgaaaacgat ggacaataac      960

ggcttgctgc gacgaacgct ggcggcgtgg tttcgccaca atgtgcaacc gctggcaacg     1020

tcaaaggcgt tgtttattca tcgtaatacc ctggagtatc ggcttaatcg tatatcggaa     1080

ctgaccgggc ttgatttggg caattttgat gacaggttgc tgctgtatgt ggcgttacaa     1140

ctggatgaag agcggtag                                                   1158


<210>  12
<211>  585
<212>  DNA
<213>  Artificial

<220>
<223>  Regulator

<400>  12
atgccgcgtc cgaaactgaa atctgacgac gaagttctgg aagcggcgac cgttgttctg       60

aaacgttgcg gtccgatcga attcaccctg tctggtgttg cgaaagaagt tggtctgtct      120

cgtgcggcgc tgatccagcg tttcaccaac cgtgacaccc tgctggttcg tatgatggaa      180

cgtggtgttg aacaggttcg tcactacctg aacgcgatcc cgatcggtgc gggtccgcag      240

ggtctgtggg aattcctgca ggttctggtt cgttctatga acacccgtaa cgacttctct      300

gttaactacc tgatctcttg gtacgaactg caggttccgg aactgcgtac cctggcgatc      360

cagcgtaacc gtgcggttgt tgaaggtatc cgtaaacgtc tgccgccggg tgcgccggcg      420

gcggcggaac tgctgctgca ctctgttatc gcgggtgcga ccatgcagtg ggcggttgac      480

ccggacggtg aactggcgga ccacgttctg gcgcagatcg cggcgatcct gtgcctgatg      540

ttcccggaac acgacgactt ccagctgctg caggcgcacg cgtaa                      585


<210>  13
<211>  624
<212>  DNA
<213>  Artificial

<220>
<223>  Regulator

<400>  13
atgtctcgtt tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc       60

ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca      120

ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta      180

gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt      240

aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat      300

ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta      360

tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgcagt ggggcatttt      420

actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca      480

cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa      540

ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa      600

cttaaatgtg aaagtgggtc ttaa                                             624


<210>  14
<211>  633
<212>  DNA
<213>  Artificial

<220>
<223>  Regulator

<400>  14
atggtgcgtc gcaccaaaga agaagcacag gaaacgcgtg cgcagattat cgaagcggcc       60

gaacgcgcgt tttataaacg tggtgtggca cgtaccacgc tggcagatat tgcagaactg      120

gcaggtgtta cccgcggtgc aatctactgg catttcaaca ataaagccga actggttcag      180

gcactgctgg attctctgca cgaaacgcat gatcacctgg cccgtgcaag cgaatctgaa      240

gatgaactgg acccgctggg ctgcatgcgc aaactgctgc tgcaggtgtt taacgaactg      300

gttctggatg cacgtacccg tcgcattaat gaaatcctgc atcacaaatg cgaatttacg      360

gatgatatgt gtgaaattcg tcagcagcgc cagagcgccg tgctggattg tcataaaggt      420

atcaccctgg cactggcaaa cgcagttcgt cgcggtcagc tgccgggtga actggatgtg      480

gaacgcgcag cggttgcgat gtttgcctat gtggatggcc tgattggtcg ttggctgctg      540

ctgccggata gtgttgatct gctgggcgat gtggaaaaat gggttgatac cggtctggat      600

atgctgcgtc tgagcccggc gctgcgcaaa taa                                   633


<210>  15
<211>  1023
<212>  DNA
<213>  Artificial

<220>
<223>  MIOX variant

<400>  15
atggtaaaca aggtcggtaa atctactctc gataagagca caaacctaga taaatccaaa       60

gggaatatat tagagaaact agatgatgat atacttcatg tcaatagaat tcgaggctct      120

ttaactaaca aaactccaat caccaaaacc cattcgatag atgatgagct taaactagaa      180

gaacaatcag aaactgccgc cgatgaaaat tggcaaatag catcggaata ttataaaaac      240

atagacacga aggctttccg ccaatatgaa ttagcttgtg atagagtcaa acagttttat      300

gaagaacaac atgaaaaaca aaccgtggcg tataatattc aagcaagaat taatttcaaa      360

actaaaacaa gagcaagaat gacagtttgg gaaggactag agaaattaaa caaattgtta      420

gatgattctg atcccgacac cgaattgtca caaatagatc atgcattaca gacggcagaa      480

gctatacggc gagatgggaa accacgatgg tttcaattag ttgggttgat tcatgattta      540

gggaaattac tatatttttt tgattctcgt ggtcaatggg atgtagtggg tgatactttc      600

cctgttggtt gtaaattcct gaaacggatt attttccctg atagttttaa aaataatcca      660

gatttcctaa atccattgta taataccaaa tatggcatat attcaaaaca ttgtggatta      720

gataaagtca tgttgagttg gggtcatgat gagtatatgt atcatgttgc gaaaaagaat      780

tcgacattac caccggaagc attggcaatg ataaggtatc attcatttta tccttggcat      840

caagaattgg catatagtta tttaatggat gagcatgata aagagatgtt gaaagcagtc      900

aaagctttca attcctatga tttatattcc aagatagatc aacagtatga tgttgaagag      960

ttgaaaccat attacctaga gttgattgat gagtttttcc caaataaagt aattgatttt     1020

taa                                                                   1023


<210>  16
<211>  978
<212>  DNA
<213>  Artificial

<220>
<223>  MIOX variant

<400>  16
atgagtcaga ccgtggaaaa cacgtttggc gaatttcgta actacaccga tagcaaattc       60

caggatcgtg tggaacgcac gtacaaagat atgcacatta accagaatct ggaatacgtt      120

acccagatga aagataaata cttcaaactg gatctgggta aaatggatgt gtacgaagtt      180

ttcaaactgc tggaaaacgt tcatgatgaa agcgatccgg ataatgatct gccgcagatc      240

gaacacgcat atcagaccgc ggaagcctgc cagaacaaat tcctgaaatc tgatacggaa      300

ctgcgcgaaa atgcgctgat tcgtagtatc tttcgcgatc atgaatggca gagcattccg      360

aaaatctggc aggatttcta taccaaaaaa cagagtctgg gcaatctgta cagccatatt      420

aaagattggt cttggtttcc gctggttggc ttcgttcacg atctgggtaa aatcatgacc      480

ctgccggaat atggtcagct gccgcagtgg agcaccgtgg gtgatacgta cccgattgcc      540

tgcccgtttg caagcgcgaa cgtgttttct caccgtgaat ttgttaaaga ttctaaagat      600

tacaacaatt acaataccga aagtgaaacg cattatggca aatacgagaa aaaatgtggt      660

ttcgataacg tggatatgag cttcggtcac gatgaataca tctacaaagt tttcgaacag      720

ggcagcgata tcccgtatga aggtctgtac ctgctgcgct atcattcttt ctacccgtgg      780

cacaccccgc agacgggcgg tcatgcgtat caggaactgg ccaacgaaaa agattggctg      840

ctgctgccgc tgctgaaagc ctttcagaaa gcggatctgt attctaaact gccggaactg      900

ccgccgaaag aagtgctgga gaaaaaatac aaaagtctgc tggataaatg ggttccgaac      960

aagaaaatta actggtaa                                                    978


<210>  17
<211>  888
<212>  DNA
<213>  Artificial

<220>
<223>  MIOX variant

<400>  17
atgaaaaagc atatagacac agacaatccg ttgaaaaatt tagatgagtg ggaagatgat       60

ttgttaatgc gatatcctga cccttctgaa gtaaatgaaa gtttaaaaga aaagcagaaa      120

gaagaattta gaaattatgt cgattctgaa agagtagaaa cggtaaaaga attttacagg      180

ataaaccata cctaccaaac ttatgacttt gtatgcagta aagaacaaga atttctgcaa      240

tttaatagaa aagaaatgtc aatctgggaa gctgtcgagt ttttaaacac gcttgtagac      300

gacagtgacc cagatattga cttagaccag acacagcacc ttttacagac ttcagaagcc      360

attcgtgctg atggtcatcc ggattggttt gtactgacag gtttcattca cgatttgggt      420

aaagttttat gcttatttgg agaaccgcaa tgggcagtcg ttggcgatac ttttccggtt      480

ggctgtgcgt attcggataa aattgtgtat tcagaatttt ttaaagaaaa tccggattat      540

acagatgaga gattcaatac taaactagga atctacactg aaaactgcgg attagataac      600

gtaaaaatga gctggggtca tgacgaatat ttgtatcaga ttatgaaaga ttatttaccg      660

gatcctgctt tatacatgat tcgttatcac tctttttatt cgcagcataa agaaaatgcg      720

tatgcacatt taatgaatga aaaagacatc gaaatgtttg actgggttcg aaaattcaat      780

ccgtacgatt tgtatacaaa ggctcctgta aaaccagatg ttcaggcatt acttccttat      840

tataaagaat tagttgctaa atatttgcct gaaaaattga agttttaa                   888


<210>  18
<211>  858
<212>  DNA
<213>  Artificial

<220>
<223>  MIOX variant

<400>  18
atgaaagtgg atgttggccc ggacccgagc ctggtttacc gcccggatgt ggacccggaa       60

atggcaaaaa gcaaagattc gtttcgtaac tacaccagtg gcccgctgct ggatcgtgtt      120

tttaccacgt ataaactgat gcatacccac cagacggttg actttgtcag ccgtaaacgc      180

attcaatatg gcggtttctc ttacaagaaa atgaccatca tggaagcggt gggcatgctg      240

gatgacctgg ttgatgaatc agatccggac gtcgattttc cgaattcgtt tcatgcgttc      300

cagacggccg aaggtattcg caaagcccac ccggacaaag attggttcca tctggtcggc      360

ctgctgcacg atctgggtaa aatcatggca ctgtggggtg aaccgcagtg ggctgtggtt      420

ggtgatacct ttccggtggg ttgccgtccg caagcaagtg tcgtgttttg tgactccacc      480

ttccaggaca acccggatct gcaagacccg cgctattcaa cggaactggg catgtaccag      540

ccgcattgcg gtctggaaaa cgtgctgatg tcgtggggtc acgatgaata cctgtaccag      600

atgatgaaat tcaacaaatt cagcctgccg tctgaagcct tctacatgat ccgtttccat      660

agtttctacc cgtggcacac cggcggtgat tatcgccagc tgtgctccca gcaagacctg      720

gatatgctgc cgtgggtgca agaattcaac aaattcgatc tgtacacgaa atgtccggat      780

ctgccggacg ttgaatctct gcgtccgtac taccaaggtc tgattgataa atactgtccg      840

ggcaccctgt cgtggtaa                                                    858


