                         SEQUENCE LISTING

<110>  The Board of Trustees of the Leland Stanford Junior 
       University
       Giaccia, Amato J.
       Kariolis, Mihalis S.
       Aguilera, Todd A.
 
<120>  RECEPTOR-BASED ANTAGONISTS OF THE PROGRAMMED CELL DEATH 1 (PD-1) 
       PATHWAY

<130>  079445-000410PC-1004540

<140>  PCT/US16/26168
<141>  2016-04-06

<150>  US 62/143,695
<151>  2015-04-06

<160>  29    

<170>  PatentIn version 3.5

<210>  1
<211>  288
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln 
1               5                   10                  15      


Leu Gly Trp Arg Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp 
            20                  25                  30          


Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
        35                  40                  45              


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
    50                  55                  60                  


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
65                  70                  75                  80  


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg 
                85                  90                  95      


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
            100                 105                 110         


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             


Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
    130                 135                 140                 


Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro 
145                 150                 155                 160 


Arg Pro Ala Gly Gln Phe Gln Thr Leu Val Val Gly Val Val Gly Gly 
                165                 170                 175     


Leu Leu Gly Ser Leu Val Leu Leu Val Trp Val Leu Ala Val Ile Cys 
            180                 185                 190         


Ser Arg Ala Ala Arg Gly Thr Ile Gly Ala Arg Arg Thr Gly Gln Pro 
        195                 200                 205             


Leu Lys Glu Asp Pro Ser Ala Val Pro Val Phe Ser Val Asp Tyr Gly 
    210                 215                 220                 


Glu Leu Asp Phe Gln Trp Arg Glu Lys Thr Pro Glu Pro Pro Val Pro 
225                 230                 235                 240 


Cys Val Pro Glu Gln Thr Glu Tyr Ala Thr Ile Val Phe Pro Ser Gly 
                245                 250                 255     


Met Gly Thr Ser Ser Pro Ala Arg Arg Gly Ser Ala Asp Gly Pro Arg 
            260                 265                 270         


Ser Ala Gln Pro Leu Arg Pro Glu Asp Gly His Cys Ser Trp Pro Leu 
        275                 280                 285             


<210>  2
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic wild-type human programmed cell death 1 receptor (PD-1)
       polypeptide fragment

<400>  2

Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
1               5                   10                  15      


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
            20                  25                  30          


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
        35                  40                  45              


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg 
    50                  55                  60                  


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
65                  70                  75                  80  


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
                85                  90                  95      


Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
            100                 105                 110         


Thr Glu Arg Arg Ala Glu 
        115             


<210>  3
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic PD-1 variant polypeptide

<400>  3

Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
1               5                   10                  15      


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
            20                  25                  30          


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
        35                  40                  45              


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Ser Arg Phe Arg 
    50                  55                  60                  


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
65                  70                  75                  80  


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
                85                  90                  95      


Ala Pro Lys Val Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
            100                 105                 110         


Thr Glu Arg Arg Ala Glu 
        115             


<210>  4
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic PD-1 variant polypeptide

<400>  4

Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
1               5                   10                  15      


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
            20                  25                  30          


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
        35                  40                  45              


Ala Phe Pro Glu Asp Arg Gly Gln Pro Gly Gln Asp Ser Arg Phe Arg 
    50                  55                  60                  


Val Ile Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
65                  70                  75                  80  


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
                85                  90                  95      


Ala Pro Lys Val Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
            100                 105                 110         


Thr Glu Arg Arg Ala Glu 
        115             


<210>  5
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic PD-1 variant polypeptide

<400>  5

Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
1               5                   10                  15      


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
            20                  25                  30          


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
        35                  40                  45              


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Ser Arg Phe Arg 
    50                  55                  60                  


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
65                  70                  75                  80  


Ala Arg Arg Ser Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
                85                  90                  95      


Ala Pro Lys Val Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
            100                 105                 110         


Ile Glu Arg Arg Ala Glu 
        115             


<210>  6
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic PD-1 variant polypeptide

<400>  6

Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
1               5                   10                  15      


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
            20                  25                  30          


Leu Asn Trp Tyr Arg Val Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
        35                  40                  45              


Ala Phe Pro Glu Asp Arg Ser Arg Pro Gly Gln Asp Ser Arg Phe Arg 
    50                  55                  60                  


Val Thr Arg Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Gly 
65                  70                  75                  80  


Ala Arg Arg Ser Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
                85                  90                  95      


Ala Pro Lys Val Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
            100                 105                 110         


Thr Glu Arg Arg Ala Glu 
        115             


<210>  7
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic PD-1 variant polypeptide

<400>  7

Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
1               5                   10                  15      


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Ala Ser Glu Ser Phe Val 
            20                  25                  30          


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asp Gln Thr Asp Lys Leu Ala 
        35                  40                  45              


Ala Phe Pro Glu Asp Arg Gly Gln Leu Gly Gln Asp Ser Arg Phe Arg 
    50                  55                  60                  


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
65                  70                  75                  80  


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
                85                  90                  95      


Ala Pro Lys Ile Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
            100                 105                 110         


Thr Glu Arg Arg Ala Glu 
        115             


<210>  8
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic PD-1 variant polypeptide

<400>  8

Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
1               5                   10                  15      


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
            20                  25                  30          


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
        35                  40                  45              


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Ser Arg Phe Arg 
    50                  55                  60                  


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe Arg Met Ser Val Val Arg 
65                  70                  75                  80  


Ala Arg Arg Ser Asp Ser Gly Thr Tyr Leu Cys Ser Ala Ile Ser Leu 
                85                  90                  95      


Ala Pro Lys Val Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
            100                 105                 110         


Thr Glu Arg Arg Ala Glu 
        115             


<210>  9
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic PD-1 variant polypeptide

<400>  9

Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
1               5                   10                  15      


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
            20                  25                  30          


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
        35                  40                  45              


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Ser Arg Phe Arg 
    50                  55                  60                  


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
65                  70                  75                  80  


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
                85                  90                  95      


Ala Pro Lys Val Gln Ile Lys Glu Ser Leu Arg Val Glu Leu Arg Val 
            100                 105                 110         


Thr Glu Arg Arg Ala Glu 
        115             


<210>  10
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic PD-1 variant polypeptide

<400>  10

Asp Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
1               5                   10                  15      


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
            20                  25                  30          


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
        35                  40                  45              


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Ser Arg Phe Arg 
    50                  55                  60                  


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
65                  70                  75                  80  


Ala Arg Arg Asp Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
                85                  90                  95      


Ala Pro Lys Ile Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
            100                 105                 110         


Thr Glu Arg Arg Ala Glu 
        115             


<210>  11
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic PD-1 variant polypeptide

<400>  11

Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
1               5                   10                  15      


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
            20                  25                  30          


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
        35                  40                  45              


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Ser Arg Phe Arg 
    50                  55                  60                  


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
65                  70                  75                  80  


Ala Arg Gly Asp Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
                85                  90                  95      


Ala Pro Lys Val Gln Ile Lys Glu Ser Leu Arg Val Glu Leu Arg Val 
            100                 105                 110         


Thr Glu Arg Arg Ala Glu 
        115             


<210>  12
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic PD-1 variant polypeptide

<400>  12

Asp Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
1               5                   10                  15      


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
            20                  25                  30          


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
        35                  40                  45              


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Ser Arg Phe Arg 
    50                  55                  60                  


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
65                  70                  75                  80  


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Leu Leu 
                85                  90                  95      


Ala Pro Arg Ile Gln Ile Arg Glu Ser Leu Gly Ala Glu Leu Arg Val 
            100                 105                 110         


Thr Glu Lys Gly Ala Glu 
        115             


<210>  13
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic PD-1 variant polypeptide

<400>  13

Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
1               5                   10                  15      


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
            20                  25                  30          


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
        35                  40                  45              


Ala Phe Pro Glu Asp Arg Ser Gln Leu Gly Gln Asp Ser Arg Phe Arg 
    50                  55                  60                  


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
65                  70                  75                  80  


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
                85                  90                  95      


Ala Pro Lys Val Gln Ile Arg Glu Ser Leu Arg Val Glu Leu Arg Val 
            100                 105                 110         


Thr Glu Arg Arg Ala Glu 
        115             


<210>  14
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic PD-1 variant polypeptide

<400>  14

Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
1               5                   10                  15      


Ser Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
            20                  25                  30          


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Ala Asp Lys Leu Ala 
        35                  40                  45              


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Ser Arg Phe Arg 
    50                  55                  60                  


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Gly 
65                  70                  75                  80  


Ala Arg Arg Ser Asp Ser Gly Thr Tyr Leu Cys Ser Ala Ile Ser Leu 
                85                  90                  95      


Ala Pro Lys Val Gln Ile Arg Glu Ser Leu Arg Ala Glu Leu Arg Val 
            100                 105                 110         


Ala Glu Arg Arg Ala Glu 
        115             


<210>  15
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic PD-1 variant polypeptide

<400>  15

Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
1               5                   10                  15      


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
            20                  25                  30          


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asp Gln Thr Asp Lys Leu Ala 
        35                  40                  45              


Ala Phe Pro Glu Asp Arg Ser Arg Pro Gly Gln Asp Ser Arg Phe Arg 
    50                  55                  60                  


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Gly 
65                  70                  75                  80  


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
                85                  90                  95      


Ala Pro Lys Val Gln Ile Arg Glu Ser Leu Arg Ala Glu Leu Arg Val 
            100                 105                 110         


Thr Glu Arg Arg Ala Glu 
        115             


<210>  16
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic PD-1 variant polypeptide

<400>  16

Asn Pro Pro Thr Leu Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
1               5                   10                  15      


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
            20                  25                  30          


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
        35                  40                  45              


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Ser Arg Phe Arg 
    50                  55                  60                  


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
65                  70                  75                  80  


Ala Arg Arg Asp Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
                85                  90                  95      


Ala Pro Lys Val Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
            100                 105                 110         


Thr Glu Arg Arg Ala Glu 
        115             


<210>  17
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic PD-1 variant polypeptide

<400>  17

Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
1               5                   10                  15      


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
            20                  25                  30          


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
        35                  40                  45              


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Ser Arg Phe Arg 
    50                  55                  60                  


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Gly 
65                  70                  75                  80  


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
                85                  90                  95      


Ala Pro Lys Ile Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
            100                 105                 110         


Thr Glu Arg Arg Ala Glu 
        115             


<210>  18
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic PD-1 variant polypeptide

<400>  18

Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Ala Glu Gly Asp 
1               5                   10                  15      


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
            20                  25                  30          


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
        35                  40                  45              


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Arg Arg Phe Arg 
    50                  55                  60                  


Val Thr Gln Leu Pro Ser Gly Arg Asp Phe His Met Ser Val Val Arg 
65                  70                  75                  80  


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Phe Leu 
                85                  90                  95      


Ala Pro Arg Ile Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
            100                 105                 110         


Thr Glu Arg Arg Ala Glu 
        115             


<210>  19
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic PD-1 variant polypeptide

<400>  19

Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
1               5                   10                  15      


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
            20                  25                  30          


Leu Asn Trp Tyr Arg Met Ser Pro Ser Ser Gln Thr Asp Lys Leu Ala 
        35                  40                  45              


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Ser Arg Phe Arg 
    50                  55                  60                  


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
65                  70                  75                  80  


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
                85                  90                  95      


Ala Pro Lys Val Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
            100                 105                 110         


Thr Glu Arg Arg Ala Glu 
        115             


<210>  20
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic PD-1 variant polypeptide

<400>  20

Ser Pro Pro Thr Leu Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
1               5                   10                  15      


Asn Val Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
            20                  25                  30          


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
        35                  40                  45              


Ala Phe Pro Glu Asp Arg Gly Gln Pro Gly Arg Asp Ser Arg Phe Arg 
    50                  55                  60                  


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
65                  70                  75                  80  


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
                85                  90                  95      


Ala Pro Lys Val Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
            100                 105                 110         


Thr Glu Arg Arg Ala Glu 
        115             


<210>  21
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic PD-1 variant polypeptide

<400>  21

Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
1               5                   10                  15      


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
            20                  25                  30          


Leu Asn Trp Tyr Arg Met Ser Pro Ser Ser Gln Thr Asp Lys Leu Ala 
        35                  40                  45              


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Ser Arg Phe Arg 
    50                  55                  60                  


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
65                  70                  75                  80  


Ala Arg Arg Ser Asp Ser Gly Thr Tyr Leu Cys Gly Val Ile Ser Leu 
                85                  90                  95      


Ala Pro Lys Val Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
            100                 105                 110         


Ile Glu Arg Arg Ala Glu 
        115             


<210>  22
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic PD-1 variant polypeptide

<400>  22

Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
1               5                   10                  15      


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
            20                  25                  30          


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
        35                  40                  45              


Ala Phe Pro Glu Asp Arg Gly Gln Leu Gly Gln Asp Ser Arg Phe Arg 
    50                  55                  60                  


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
65                  70                  75                  80  


Ala Arg Arg Ser Asp Ser Gly Thr Tyr Leu Cys Ser Ala Ile Val Leu 
                85                  90                  95      


Ala Pro Lys Ile Gln Ile Lys Glu Ser Leu Arg Val Glu Leu Arg Val 
            100                 105                 110         


Thr Glu Arg Arg Ala Glu 
        115             


<210>  23
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic PD-1 variant polypeptide

<400>  23

Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
1               5                   10                  15      


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
            20                  25                  30          


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
        35                  40                  45              


Ala Phe Pro Glu Asp Arg Gly Gln Pro Gly Arg Asp Ser Arg Phe Arg 
    50                  55                  60                  


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe Arg Met Ser Val Val Arg 
65                  70                  75                  80  


Ala Arg Arg Ser Asp Ser Gly Thr Tyr Leu Cys Ser Ala Ile Leu Leu 
                85                  90                  95      


Ala Pro Lys Ile Gln Ile Lys Glu Ser Leu Gly Ala Glu Leu Arg Val 
            100                 105                 110         


Thr Glu Lys Gly Ala Glu 
        115             


<210>  24
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic PD-1 variant polypeptide

<400>  24

Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
1               5                   10                  15      


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
            20                  25                  30          


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
        35                  40                  45              


Ala Phe Pro Glu Asp Arg Gly Gln Leu Gly Gln Asp Ser Arg Phe Arg 
    50                  55                  60                  


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
65                  70                  75                  80  


Ala Arg Arg Ser Asp Ser Gly Thr Tyr Leu Cys Ser Ala Ile Leu Leu 
                85                  90                  95      


Ala Pro Lys Ile Gln Ile Lys Glu Ser Leu Arg Val Glu Leu Arg Val 
            100                 105                 110         


Thr Glu Arg Arg Ala Glu 
        115             


<210>  25
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic PD-1 variant polypeptide

<400>  25

Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
1               5                   10                  15      


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
            20                  25                  30          


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
        35                  40                  45              


Ala Phe Pro Glu Asp Arg Gly Gln Leu Gly Gln Ala Ser Arg Phe Arg 
    50                  55                  60                  


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
65                  70                  75                  80  


Ala Arg Arg Ser Asp Ser Gly Thr Tyr Leu Cys Ser Ala Ile Leu Leu 
                85                  90                  95      


Ala Pro Arg Ile Gln Ile Arg Glu Ser Leu Arg Val Glu Leu Arg Val 
            100                 105                 110         


Thr Glu Arg Arg Ala Glu 
        115             


<210>  26
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic PD-1 variant polypeptide

<400>  26

Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
1               5                   10                  15      


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
            20                  25                  30          


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
        35                  40                  45              


Ala Phe Pro Glu Asp Arg Gly Gln Ser Gly Gln Gly Ser Arg Phe Gly 
    50                  55                  60                  


Val Thr Arg Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
65                  70                  75                  80  


Ala Arg Arg Ser Asp Ser Gly Thr Tyr Leu Cys Ser Ala Ile Ser Leu 
                85                  90                  95      


Ala Pro Lys Val Gln Ile Lys Glu Ser Leu Arg Val Glu Leu Arg Val 
            100                 105                 110         


Thr Glu Arg Arg Ala Glu 
        115             


<210>  27
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic PD-1 variant polypeptide

<400>  27

Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
1               5                   10                  15      


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
            20                  25                  30          


Leu Asn Trp Tyr Arg Ile Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
        35                  40                  45              


Ala Phe Pro Glu Asp Arg Gly Gln Leu Gly Gln Asp Ser Arg Phe Arg 
    50                  55                  60                  


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
65                  70                  75                  80  


Ala Arg Arg Ser Asp Ser Gly Thr Tyr Leu Cys Ser Ala Ile Leu Leu 
                85                  90                  95      


Ala Pro Arg Ile Gln Ile Arg Glu Ser Leu Gly Val Glu Leu Arg Val 
            100                 105                 110         


Thr Glu Lys Arg Ala Glu 
        115             


<210>  28
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic PD-1 variant polypeptide

<400>  28

Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
1               5                   10                  15      


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
            20                  25                  30          


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
        35                  40                  45              


Ala Phe Pro Glu Asp Arg Gly Gln Leu Gly Gln Asp Ser Arg Phe Arg 
    50                  55                  60                  


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
65                  70                  75                  80  


Ala Arg Arg Ser Asp Ser Gly Thr Tyr Leu Cys Ser Ala Ile Val Leu 
                85                  90                  95      


Ala Pro Arg Ile Gln Ile Lys Glu Ser Leu Arg Val Glu Leu Arg Val 
            100                 105                 110         


Thr Glu Arg Arg Ala Glu 
        115             


<210>  29
<211>  288
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic PD-1 variant polypeptide


<220>
<221>  VARIANT
<222>  (33)..(33)
<223>  Xaa may be Asn, Asp or Ser

<220>
<221>  VARIANT
<222>  (37)..(37)
<223>  Xaa may be Phe or Leu

<220>
<221>  VARIANT
<222>  (45)..(45)
<223>  Xaa may be Thr or Ala

<220>
<221>  VARIANT
<222>  (49)..(49)
<223>  Xaa may be Asn or Ser

<220>
<221>  VARIANT
<222>  (50)..(50)
<223>  Xaa may be Ala or Val

<220>
<221>  VARIANT
<222>  (59)..(59)
<223>  Xaa may be Thr or Ala

<220>
<221>  VARIANT
<222>  (70)..(70)
<223>  Xaa may be Met, Val or Ile

<220>
<221>  VARIANT
<222>  (74)..(74)
<223>  Xaa may be Asn, Asp or Ser

<220>
<221>  VARIANT
<222>  (76)..(76)
<223>  Xaa may be Thr or Ala

<220>
<221>  VARIANT
<222>  (87)..(87)
<223>  Xaa may be Ser or Gly

<220>
<221>  VARIANT
<222>  (88)..(88)
<223>  Xaa may be Gln or Arg

<220>
<221>  VARIANT
<222>  (89)..(89)
<223>  Xaa may be Pro, Leu or Ser

<220>
<221>  VARIANT
<222>  (91)..(91)
<223>  Xaa may be Gln or Arg

<220>
<221>  VARIANT
<222>  (92)..(92)
<223>  Xaa may be Asp, Ala or Gly

<220>
<221>  VARIANT
<222>  (93)..(93)
<223>  Xaa may be Cys, Ser or Arg

<220>
<221>  VARIANT
<222>  (96)..(96)
<223>  Xaa may be Arg or Gly

<220>
<221>  VARIANT
<222>  (98)..(98)
<223>  Xaa may be Thr or Ile

<220>
<221>  VARIANT
<222>  (99)..(99)
<223>  Xaa may be Gln, Ala or Arg

<220>
<221>  VARIANT
<222>  (102)..(102)
<223>  Xaa may be Asn or Ser

<220>
<221>  VARIANT
<222>  (107)..(107)
<223>  Xaa may be His or Arg

<220>
<221>  VARIANT
<222>  (112)..(112)
<223>  Xaa may be Arg or Gly

<220>
<221>  VARIANT
<222>  (115)..(115)
<223>  Xaa may be Arg or Gly

<220>
<221>  VARIANT
<222>  (116)..(116)
<223>  Xaa may be Asn, Asp or Ser

<220>
<221>  VARIANT
<222>  (124)..(124)
<223>  Xaa may be Gly or Ser

<220>
<221>  VARIANT
<222>  (125)..(125)
<223>  Xaa may be Ala or Val

<220>
<221>  VARIANT
<222>  (127)..(127)
<223>  Xaa may be Ser, Phe, Leu, or Val

<220>
<221>  VARIANT
<222>  (131)..(131)
<223>  Xaa may be Lys or Arg

<220>
<221>  VARIANT
<222>  (132)..(132)
<223>  Xaa may be Ala, Ile or Val

<220>
<221>  VARIANT
<222>  (135)..(135)
<223>  Xaa may be Lys or Arg

<220>
<221>  VARIANT
<222>  (139)..(139)
<223>  Xaa may be Arg or Gly

<220>
<221>  VARIANT
<222>  (140)..(140)
<223>  Xaa may be Ala or Val

<220>
<221>  VARIANT
<222>  (145)..(145)
<223>  Xaa may be Thr, Ala or Ile

<220>
<221>  VARIANT
<222>  (147)..(147)
<223>  Xaa may be Arg or Lys

<220>
<221>  VARIANT
<222>  (148)..(148)
<223>  Xaa may be Arg or Gly

<400>  29

Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln 
1               5                   10                  15      


Leu Gly Trp Arg Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp 
            20                  25                  30          


Xaa Pro Pro Thr Xaa Ser Pro Ala Leu Leu Val Val Xaa Glu Gly Asp 
        35                  40                  45              


Xaa Xaa Thr Phe Thr Cys Ser Phe Ser Asn Xaa Ser Glu Ser Phe Val 
    50                  55                  60                  


Leu Asn Trp Tyr Arg Xaa Ser Pro Ser Xaa Gln Xaa Asp Lys Leu Ala 
65                  70                  75                  80  


Ala Phe Pro Glu Asp Arg Xaa Xaa Xaa Gly Xaa Xaa Xaa Arg Phe Xaa 
                85                  90                  95      


Val Xaa Xaa Leu Pro Xaa Gly Arg Asp Phe Xaa Met Ser Val Val Xaa 
            100                 105                 110         


Ala Arg Xaa Xaa Asp Ser Gly Thr Tyr Leu Cys Xaa Xaa Ile Xaa Leu 
        115                 120                 125             


Ala Pro Xaa Xaa Gln Ile Xaa Glu Ser Leu Xaa Xaa Glu Leu Arg Val 
    130                 135                 140                 


Xaa Glu Xaa Xaa Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro 
145                 150                 155                 160 


Arg Pro Ala Gly Gln Phe Gln Thr Leu Val Val Gly Val Val Gly Gly 
                165                 170                 175     


Leu Leu Gly Ser Leu Val Leu Leu Val Trp Val Leu Ala Val Ile Cys 
            180                 185                 190         


Ser Arg Ala Ala Arg Gly Thr Ile Gly Ala Arg Arg Thr Gly Gln Pro 
        195                 200                 205             


Leu Lys Glu Asp Pro Ser Ala Val Pro Val Phe Ser Val Asp Tyr Gly 
    210                 215                 220                 


Glu Leu Asp Phe Gln Trp Arg Glu Lys Thr Pro Glu Pro Pro Val Pro 
225                 230                 235                 240 


Cys Val Pro Glu Gln Thr Glu Tyr Ala Thr Ile Val Phe Pro Ser Gly 
                245                 250                 255     


Met Gly Thr Ser Ser Pro Ala Arg Arg Gly Ser Ala Asp Gly Pro Arg 
            260                 265                 270         


Ser Ala Gln Pro Leu Arg Pro Glu Asp Gly His Cys Ser Trp Pro Leu 
        275                 280                 285             


