                         SEQUENCE LISTING

<110>  President and Fellows of Harvard College
 
<120>  BIOSYNTHETIC AMYLOID-BASED MATERIALS DISPLAYING FUNCTIONAL 
       PROTEIN SEQUENCES

<130>  010498_00779

<140>  US 62/143,560
<141>  2015-04-06

<160>  40    

<170>  PatentIn version 3.5

<210>  1
<211>  151
<212>  PRT
<213>  Escherichia coli

<400>  1

Met Lys Leu Leu Lys Val Ala Ala Ile Ala Ala Ile Val Phe Ser Gly 
1               5                   10                  15      


Ser Ala Leu Ala Gly Val Val Pro Gln Tyr Gly Gly Gly Gly Asn His 
            20                  25                  30          


Gly Gly Gly Gly Asn Asn Ser Gly Pro Asn Ser Glu Leu Asn Ile Tyr 
        35                  40                  45              


Gln Tyr Gly Gly Gly Asn Ser Ala Leu Ala Leu Gln Thr Asp Ala Arg 
    50                  55                  60                  


Asn Ser Asp Leu Thr Ile Thr Gln His Gly Gly Gly Asn Gly Ala Asp 
65                  70                  75                  80  


Val Gly Gln Gly Ser Asp Asp Ser Ser Ile Asp Leu Thr Gln Arg Gly 
                85                  90                  95      


Phe Gly Asn Ser Ala Thr Leu Asp Gln Trp Asn Gly Lys Asn Ser Glu 
            100                 105                 110         


Met Thr Val Lys Gln Phe Gly Gly Gly Asn Gly Ala Ala Val Asp Gln 
        115                 120                 125             


Thr Ala Ser Asn Ser Ser Val Asn Val Thr Gln Val Gly Phe Gly Asn 
    130                 135                 140                 


Asn Ala Thr Ala His Gln Tyr 
145                 150     


<210>  2
<211>  1997
<212>  DNA
<213>  Artificial

<220>
<223>  CsgA gene verification region


<220>
<221>  misc_feature
<222>  (1)..(9)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(19)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (36)..(36)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1981)..(1986)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1989)..(1997)
<223>  n is a, c, g, or t

<400>  2
nnnnnnnnnc gcancaganc nttctctccn ggttcntctt atgctcgata tttcaacaaa       60

ttaagacttt tctgaagagg gcagccattg ttgtgataaa tgaagtgact gtccatcaga      120

aacagtaaca actattttca cccgatcgtc cggggaaata tttaaactca acttcgtcaa      180

agcaatgggt tgattagcag gcaatgagag ggtcttttct tgcttcgtct gactttgccc      240

tgaactgcct tcgcgcaggg acaatatttg tactctgcac agacaagatt gagtaagagt      300

gacttcagga ataatggtgt acatatcccc ttgctgggtc gtattaaagg ttatctgact      360

ggaaagtgcc gcaaggagta ataacgcatt catattcttc tcccgaaaaa aaacagggct      420

tgcgccgtgt aggctggagc tgcttcgaag ttcctatact ttctagagaa taggaacttc      480

ggaataggaa cttcatttaa atggcgcgcc ttacgccccg ccctgccact catcgcagta      540

ctgttgtatt cattaagcat ctgccgacat ggaagccatc acaaacggca tgatgaacct      600

gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa      660

cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc      720

agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt      780

tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt      840

ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag      900

ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgt aattccggat      960

gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt     1020

tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt     1080

gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg     1140

tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgaca     1200

actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta     1260

cgtgccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg gtatcaacag     1320

ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtagg cgcgccgaag     1380

ttcctatact ttctagagaa taggaacttc ggaataggaa ctaaggagga tattcatatg     1440

gaccatggct aattcccatg taaaaccccc atcggattga tttaaaagtc gaatggaaat     1500

taacgttgtg tcacgcgaat agccatttgc gactgtctct gcactacaat tgccgttttt     1560

tgagtaccat actgtgtaat atttgcttta ttaccagaac ctttctggat aatcatcgca     1620

gtattaccat aagcaccttg cgaaatactg gcatcgttgg cactgcccgc ctgatcaata     1680

tatgcaaggt tataatctcc tgtctgatca atctttgccc agttgctact accttcttgc     1740

gcaacaaccg tcaaaagttt tgagcctccc tgccgtaact gagcactatt attagtccca     1800

gcttgaccaa ttatggctgc ctgattaaat gaagacttac tcaattcatt taccgcaaag     1860

ttatattctg aattagctaa atcataacct gctgcggctg caatcccagg cgcacccagt     1920

attgttaaca tcataaataa caatttgttt ttcatgttgt caccctggac ctggtcgtac     1980

nnnnnnaann nnnnnnn                                                    1997


<210>  3
<211>  19
<212>  PRT
<213>  Artificial

<220>
<223>  linker


<220>
<221>  misc_feature
<222>  (16)..(19)
<223>  Xaa can be any naturally occurring amino acid

<400>  3

Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Xaa 
1               5                   10                  15      


Xaa Xaa Xaa 
            


<210>  4
<211>  4
<212>  PRT
<213>  Artificial

<220>
<223>  linker

<400>  4

Gly Gly Gly Ser 
1               


<210>  5
<211>  12
<212>  PRT
<213>  Artificial

<220>
<223>  linker

<400>  5

Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
1               5                   10          


<210>  6
<211>  24
<212>  PRT
<213>  Artificial

<220>
<223>  linker

<400>  6

Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Ser 
            20                  


<210>  7
<211>  48
<212>  PRT
<213>  Artificial

<220>
<223>  linker

<400>  7

Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
            20                  25                  30          


Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
        35                  40                  45              


<210>  8
<211>  12
<212>  PRT
<213>  Artificial

<220>
<223>  linker

<400>  8

Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro 
1               5                   10          


<210>  9
<211>  24
<212>  PRT
<213>  Artificial

<220>
<223>  linker

<400>  9

Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro 
1               5                   10                  15      


Pro Pro Pro Pro Pro Pro Pro Pro 
            20                  


<210>  10
<211>  15
<212>  PRT
<213>  Artificial

<220>
<223>  linker

<400>  10

Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
1               5                   10                  15  


<210>  11
<211>  45
<212>  PRT
<213>  Artificial

<220>
<223>  linker

<400>  11

Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu 
1               5                   10                  15      


Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala 
            20                  25                  30          


Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
        35                  40                  45  


<210>  12
<211>  80
<212>  PRT
<213>  Artificial

<220>
<223>  linker

<400>  12

Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
            20                  25                  30          


Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
        35                  40                  45              


Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
    50                  55                  60                  


Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
65                  70                  75                  80  


<210>  13
<211>  30
<212>  PRT
<213>  Artificial

<220>
<223>  linker_nucleotides 1 to 29 may be absent

<400>  13

Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro 
1               5                   10                  15      


Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro 
            20                  25                  30  


<210>  14
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  linker

<400>  14

Glu Ala Ala Ala Lys 
1               5   


<210>  15
<211>  75
<212>  PRT
<213>  Artificial

<220>
<223>  linker_EAAAK may be absent 1 to 14 times

<400>  15

Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu 
1               5                   10                  15      


Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala 
            20                  25                  30          


Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala 
        35                  40                  45              


Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala 
    50                  55                  60                  


Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
65                  70                  75  


<210>  16
<211>  48
<212>  PRT
<213>  Artificial

<220>
<223>  linker

<400>  16

Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
            20                  25                  30          


Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
        35                  40                  45              


<210>  17
<211>  24
<212>  PRT
<213>  Artificial

<220>
<223>  linker

<400>  17

Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro 
1               5                   10                  15      


Pro Pro Pro Pro Pro Pro Pro Pro 
            20                  


<210>  18
<211>  45
<212>  PRT
<213>  Artificial

<220>
<223>  linker

<400>  18

Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu 
1               5                   10                  15      


Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala 
            20                  25                  30          


Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
        35                  40                  45  


<210>  19
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  binding domain

<400>  19

Val His Leu Gly Tyr Ala Thr 
1               5           


<210>  20
<211>  12
<212>  PRT
<213>  Artificial

<220>
<223>  binding domain

<400>  20

Leu Glu Thr Thr Cys Ala Ser Leu Cys Tyr Pro Ser 
1               5                   10          


<210>  21
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  binding domain

<400>  21

Val Arg Pro Met Pro Leu Gln 
1               5           


<210>  22
<211>  12
<212>  PRT
<213>  Artificial

<220>
<223>  binding domain

<400>  22

Leu Thr His Pro Gln Asp Ser Pro Pro Ala Ser Ala 
1               5                   10          


<210>  23
<211>  106
<212>  PRT
<213>  Artificial

<220>
<223>  binding domain

<400>  23

Glu Lys Pro Ala Ala Cys Arg Cys Ser Arg Gln Asp Pro Lys Asn Arg 
1               5                   10                  15      


Val Asn Cys Gly Phe Pro Gly Ile Thr Ser Asp Gln Cys Phe Thr Ser 
            20                  25                  30          


Gly Cys Cys Phe Asp Ser Gln Val Pro Gly Val Pro Trp Cys Phe Lys 
        35                  40                  45              


Pro Leu Pro Ala Gln Glu Ser Glu Glu Cys Val Met Gln Val Ser Ala 
    50                  55                  60                  


Arg Lys Asn Cys Gly Tyr Pro Gly Ile Ser Pro Glu Asp Cys Ala Ala 
65                  70                  75                  80  


Arg Asn Cys Cys Phe Ser Asp Thr Ile Pro Glu Val Pro Trp Cys Phe 
                85                  90                  95      


Phe Pro Met Ser Val Glu Asp Cys His Tyr 
            100                 105     


<210>  24
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  binding domain

<400>  24

Glu Glu Tyr Val Gly Leu Ser Ala Asn Gln Cys Ala Val Pro Ala Lys 
1               5                   10                  15      


Asp Arg Val Asp Cys Gly Tyr Pro His Val Thr Pro Lys Glu Cys Asn 
            20                  25                  30          


Asn Arg Gly Cys Cys Phe Asp Ser Arg Ile Pro Gly Val Pro Trp Cys 
        35                  40                  45              


Phe Lys Pro Leu Gln Glu Ala Glu Cys Thr Phe 
    50                  55                  


<210>  25
<211>  12
<212>  PRT
<213>  Artificial

<220>
<223>  peptide domain

<400>  25

Leu Thr His Pro Gln Asp Ser Pro Pro Ala Ser Ala 
1               5                   10          


<210>  26
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  peptide domain

<400>  26

Val His Leu Gly Tyr Ala Thr 
1               5           


<210>  27
<211>  12
<212>  PRT
<213>  Artificial

<220>
<223>  peptide domain

<400>  27

Leu Glu Thr Thr Cys Ala Ser Leu Cys Tyr Pro Ser 
1               5                   10          


<210>  28
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  peptide domain

<400>  28

Val Arg Pro Met Pro Leu Gln 
1               5           


<210>  29
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  proline sequence


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  29

Leu Pro Xaa Thr Gly 
1               5   


<210>  30
<211>  19
<212>  PRT
<213>  Artificial

<220>
<223>  linker_GGS repeat may be absent 3 to 4 times


<220>
<221>  misc_feature
<222>  (16)..(19)
<223>  Xaa can be any naturally occurring amino acid

<400>  30

Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Xaa 
1               5                   10                  15      


Xaa Xaa Xaa 
            


<210>  31
<211>  43
<212>  PRT
<213>  Artificial

<220>
<223>  binding domain

<400>  31

Ser Lys Trp Gln His Gln Gln Asp Ser Cys Arg Lys Gln Leu Gln Gly 
1               5                   10                  15      


Val Asn Leu Thr Pro Cys Glu Lys His Ile Met Glu Lys Ile Gln Gly 
            20                  25                  30          


Arg Gly Asp Asp Asp Asp Asp Asp Asp Asp Asp 
        35                  40              


<210>  32
<211>  134
<212>  PRT
<213>  Artificial

<220>
<223>  binding domain

<400>  32

Met Met Met Pro Ala Asn Tyr Ser Val Ile Ala Glu Asn Glu Met Thr 
1               5                   10                  15      


Tyr Val Asn Gly Gly Ala Asn Phe Ile Asp Ala Ile Gly Ala Val Thr 
            20                  25                  30          


Ala Pro Ile Trp Thr Leu Asp Asn Val Lys Thr Phe Asn Thr Asn Ile 
        35                  40                  45              


Val Thr Leu Val Gly Asn Thr Phe Leu Gln Ser Thr Ile Asn Arg Thr 
    50                  55                  60                  


Ile Val Leu Phe Ser Gly Asn Thr Thr Trp Lys Glu Val Gly Asn Ile 
65                  70                  75                  80  


Gly Lys Asn Leu Phe Gly Thr Asn Val Lys Gly Asn Pro Ile Glu Lys 
                85                  90                  95      


Asn Asn Phe Gly Asp Tyr Ala Met Asn Ala Leu Gly Ile Ala Ala Ala 
            100                 105                 110         


Val Tyr Asn Leu Gly Val Ala Pro Thr Lys Asn Thr Val Lys Glu Thr 
        115                 120                 125             


Glu Val Lys Phe Thr Val 
    130                 


<210>  33
<211>  18
<212>  PRT
<213>  Artificial

<220>
<223>  binding peptide

<400>  33

Asp Trp Phe Lys Ala Phe Tyr Asp Lys Val Ala Glu Lys Phe Lys Glu 
1               5                   10                  15      


Ala Phe 
        


<210>  34
<211>  12
<212>  PRT
<213>  Artificial

<220>
<223>  binding peptide

<400>  34

Gly Gly Ala Val Val Thr Gly Val Thr Ala Val Ala 
1               5                   10          


<210>  35
<211>  12
<212>  PRT
<213>  Artificial

<220>
<223>  binding peptide

<400>  35

Gly Gly Ala Val Val Thr Gly Val Thr Ala Val Ala 
1               5                   10          


<210>  36
<211>  12
<212>  PRT
<213>  Artificial

<220>
<223>  binding peptide

<400>  36

Leu Thr His Pro Gln Asp Ser Pro Pro Ala Ser Ala 
1               5                   10          


<210>  37
<211>  220
<212>  PRT
<213>  Artificial

<220>
<223>  binding peptide

<400>  37

Leu Val Val Ala Thr Asp Thr Ala Phe Val Pro Phe Glu Phe Lys Gln 
1               5                   10                  15      


Gly Asp Lys Tyr Val Gly Phe Asp Val Asp Leu Trp Ala Ala Ile Ala 
            20                  25                  30          


Lys Glu Leu Lys Leu Asp Tyr Glu Leu Lys Pro Met Asp Phe Ser Gly 
        35                  40                  45              


Ile Ile Pro Ala Leu Gln Thr Lys Asn Val Asp Ala Leu Ala Gly Ile 
    50                  55                  60                  


Thr Ile Thr Asp Glu Arg Lys Lys Ala Ile Asp Phe Ser Asp Gly Tyr 
65                  70                  75                  80  


Tyr Lys Ser Gly Leu Leu Val Met Val Lys Ala Asn Asn Asn Asp Val 
                85                  90                  95      


Lys Ser Val Lys Asp Leu Asp Gly Lys Val Val Ala Val Lys Ser Gly 
            100                 105                 110         


Thr Gly Ser Val Asp Tyr Ala Lys Ala Asn Ile Lys Thr Lys Asp Leu 
        115                 120                 125             


Arg Gln Phe Pro Asn Ile Asp Asn Ala Tyr Met Glu Leu Gly Thr Asn 
    130                 135                 140                 


Arg Ala Asp Ala Val Leu His Asp Thr Pro Asn Ile Leu Tyr Phe Ile 
145                 150                 155                 160 


Lys Thr Ala Gly Asn Gly Gln Phe Lys Ala Val Gly Asp Ser Leu Glu 
                165                 170                 175     


Ala Gln Tyr Gly Ile Ala Phe Pro Lys Gly Ser Asp Glu Leu Arg Asp 
            180                 185                 190         


Lys Val Asn Gly Ala Leu Lys Thr Leu Arg Glu Asn Gly Thr Tyr Asn 
        195                 200                 205             


Glu Ile Tyr Lys Lys Trp Phe Gly Thr Glu Pro Lys 
    210                 215                 220 


<210>  38
<211>  60
<212>  PRT
<213>  Artificial

<220>
<223>  binding domain

<400>  38

Glu Ala Gln Thr Glu Thr Cys Thr Val Ala Pro Arg Glu Arg Gln Asn 
1               5                   10                  15      


Cys Gly Phe Pro Gly Val Thr Pro Ser Gln Cys Ala Asn Lys Gly Cys 
            20                  25                  30          


Cys Phe Asp Asp Thr Val Arg Gly Val Pro Trp Cys Phe Tyr Pro Asn 
        35                  40                  45              


Thr Ile Asp Val Pro Pro Glu Glu Glu Cys Glu Phe 
    50                  55                  60  


<210>  39
<211>  48
<212>  PRT
<213>  Artificial

<220>
<223>  Fusion domain linker_GGGS repeat can be 48, 36, or 24 AA

<400>  39

Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
            20                  25                  30          


Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
        35                  40                  45              


<210>  40
<211>  36
<212>  PRT
<213>  Artificial

<220>
<223>  linker_GGGS repeat can be 36, 24, or 12 AA

<400>  40

Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
            20                  25                  30          


Gly Gly Gly Ser 
        35      


