                         SEQUENCE LISTING

<110>  Teknologian tutkimuskeskus VTT Oy
 
<120>  Enhanced diacid production with genetically modified 
       micro-organisms

<130>  2140055PC

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  948
<212>  DNA
<213>  Yarrowia lipolytica

<400>  1
atggcagcta tttccaaaga ctatgttctg tcgccctggg gcaaagccgt ggctggtgcc       60

gctggagccg tgctcgcaaa cacactggtc taccccctag acattgtgaa aacgcggctt      120

caggtgcagg tcaagcgcaa ggagggcgga ccccttcctg ccttcgagga gggccatttc      180

gagcactacg agggcactgt cgacgctctg aaaaagatct atgccgccaa cggcctggcc      240

ggcctgtacc agggtcttcc ctcttgtctt ctaggtgtgg cctctaccaa ctttgcctac      300

ttctactggt atggcttcat ccgagactct tacattaagc gaaaccccgg taaagctctc      360

tcgactccca tcgagctgct cctcggagct gtggcaggag ccctggcaca ggttttcacc      420

atccccgtgg ccgtgatcac cacacgacag cagacttcag acgccaagtc gcggcaggga      480

ttcctggcta ctgccaagag cgtggtggat gacgacggaa tcagtggtct ctggcgaggt      540

ctcaaggcct ctctggtgct ggtgatcaac ccctccatca cttacggctc gtttgagcgt      600

ctcagaacca tcctgttcaa gggtaagctg cacctgtctc ccggagagaa cttcctgctg      660

ggagccttgt ccaaggctat ggccaccatt gccactcagc ccatgattgt ggccaaggtc      720

atgcagcagt ccaagaccaa gggtggcaag cagttcaaca gctttgtgca ggcattggtg      780

ttcctgttca aggaggaggg tattttggga atgtggaagg gagttggccc ccagatctcc      840

aagggcatca ttgtgcaggg gctcctcttc atgattaagg accaagtcga gctcttcatc      900

gtgctacttt ttagactcat gaaggccccc accttgatca agggataa                   948


<210>  2
<211>  1002
<212>  DNA
<213>  Pichia guilliermondii

<400>  2
atgtcactta ctcctttcga aaaagcagcc agtggagcgt tggcgtctgc cattgcaaat       60

accctcgttt atcccttaga tctcagcaag acgttgatcc agactcaggt taaaccgagg      120

gctattggga tgaacccttc ggactccaca gcgtcaatag cttcggatac ctcggatccc      180

gaagaaagtg tttggcacca gaagcaggga aaggatggtc agttgcggta caagagcacc      240

gtggacgcgt tggctcagat atacaagaag aaaggcattt tggggtggta tcatggactc      300

atctcatcaa ttttgggttc ggcggcgcaa aatttcagtt atttttactg gtatacaatc      360

gtcaaacgag tttacgctcg tataaacaaa cacattccca atcatcgacc aggaacagcc      420

acagagttgt ttttgggagc attggctgcg gccatttctc aacttttcac catgccaatt      480

ggtgtcatca ccacccacca acaaacagat aaacaccaca agggcttagt gcagttgaca      540

agagaaatct tggaacacga cggtatctcc ggcttgtgga agggattgag ggtgtcactt      600

gtgttgtgta tcaacccttc catcacctat ggatcgtatg agagattgaa gcaaattatt      660

tatgcaaaca aggaatattt gggtcctttg gaaagtttca gtattggagt cattgccaag      720

tctatggcaa cactcgctac acagccattg attgtttcta aggccatgtt gcaaaaaaag      780

tcacatgtca agcccggtga aattgatgat ggagatgtca aattcgacaa tttccaagat      840

gcattggccc atttgtggca cacagagaaa cttgctggct tatacaaggg catagctccc      900

caattggtca agggagtgtt tgttcaaggt ttattattca tgttcaaaga ccagatcgac      960

ttggtatttt tgtcgttgtt gaagattgca aagaaaaagt ag                        1002


<210>  3
<211>  987
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  3
atgttaactc tagagtctgc attaactggc gctgtggctt cggcaatggc caatattgca       60

gtttatccgc tggatttatc gaagacgatc attcagtcac aagtatctcc ttcttcaagt      120

gaggatagta acgaaggtaa agttttgccc aataggagat ataagaatgt tgtagattgc      180

atgataaaca tattcaaaga aaagggtatt ttgggtctgt atcaaggtat gacagtcact      240

acggtggcca catttgtcca gaattttgtt tatttctttt ggtacacatt tatcagaaag      300

tcctacatga aacataagct gttaggactg caatcactga aaaaccgcga tggtcctatc      360

acaccttcta cgattgaaga attggtactt ggggtagcag ctgccagtat atcgcaactt      420

tttactagtc ccatggctgt ggtagctaca agacaacaaa cagtccattc tgcagagtct      480

gccaaattta ccaacgttat taaggacatt taccgtgaaa ataatgggga cataactgct      540

ttttggaaag gtttaagaac aggtttagca ttgacgataa atccttccat cacatatgcc      600

tcttttcaaa gacttaaaga agtttttttc catgaccatt ccaacgatgc tggcagtttg      660

tcagcagtgc aaaatttcat tttgggtgtc ctttccaaga tgatttcgac tctagttacg      720

caacccttga ttgtcgctaa agcaatgctt caaagcgctg gctctaaatt cactactttc      780

caagaagcgc tactatactt gtacaaaaat gaagggttaa aatctctttg gaagggagtt      840

cttcctcaat tgacaaaggg tgtcattgtg caaggtctgt tgtttgcttt cagaggagaa      900

ttgacaaaat ctttaaagag gctaatattc ttgtactctt cttttttcct aaagcacaac      960

ggacaacgca agctggcttc cacttga                                          987


<210>  4
<211>  315
<212>  PRT
<213>  Yarrowia lipolytica

<400>  4

Met Ala Ala Ile Ser Lys Asp Tyr Val Leu Ser Pro Trp Gly Lys Ala 
1               5                   10                  15      


Val Ala Gly Ala Ala Gly Ala Val Leu Ala Asn Thr Leu Val Tyr Pro 
            20                  25                  30          


Leu Asp Ile Val Lys Thr Arg Leu Gln Val Gln Val Lys Arg Lys Glu 
        35                  40                  45              


Gly Gly Pro Leu Pro Ala Phe Glu Glu Gly His Phe Glu His Tyr Glu 
    50                  55                  60                  


Gly Thr Val Asp Ala Leu Lys Lys Ile Tyr Ala Ala Asn Gly Leu Ala 
65                  70                  75                  80  


Gly Leu Tyr Gln Gly Leu Pro Ser Cys Leu Leu Gly Val Ala Ser Thr 
                85                  90                  95      


Asn Phe Ala Tyr Phe Tyr Trp Tyr Gly Phe Ile Arg Asp Ser Tyr Ile 
            100                 105                 110         


Lys Arg Asn Pro Gly Lys Ala Leu Ser Thr Pro Ile Glu Leu Leu Leu 
        115                 120                 125             


Gly Ala Val Ala Gly Ala Leu Ala Gln Val Phe Thr Ile Pro Val Ala 
    130                 135                 140                 


Val Ile Thr Thr Arg Gln Gln Thr Ser Asp Ala Lys Ser Arg Gln Gly 
145                 150                 155                 160 


Phe Leu Ala Thr Ala Lys Ser Val Val Asp Asp Asp Gly Ile Ser Gly 
                165                 170                 175     


Leu Trp Arg Gly Leu Lys Ala Ser Leu Val Leu Val Ile Asn Pro Ser 
            180                 185                 190         


Ile Thr Tyr Gly Ser Phe Glu Arg Leu Arg Thr Ile Leu Phe Lys Gly 
        195                 200                 205             


Lys Leu His Leu Ser Pro Gly Glu Asn Phe Leu Leu Gly Ala Leu Ser 
    210                 215                 220                 


Lys Ala Met Ala Thr Ile Ala Thr Gln Pro Met Ile Val Ala Lys Val 
225                 230                 235                 240 


Met Gln Gln Ser Lys Thr Lys Gly Gly Lys Gln Phe Asn Ser Phe Val 
                245                 250                 255     


Gln Ala Leu Val Phe Leu Phe Lys Glu Glu Gly Ile Leu Gly Met Trp 
            260                 265                 270         


Lys Gly Val Gly Pro Gln Ile Ser Lys Gly Ile Ile Val Gln Gly Leu 
        275                 280                 285             


Leu Phe Met Ile Lys Asp Gln Val Glu Leu Phe Ile Val Leu Leu Phe 
    290                 295                 300                 


Arg Leu Met Lys Ala Pro Thr Leu Ile Lys Gly 
305                 310                 315 


<210>  5
<211>  333
<212>  PRT
<213>  Pichia guilliermondii

<400>  5

Met Ser Leu Thr Pro Phe Glu Lys Ala Ala Ser Gly Ala Leu Ala Ser 
1               5                   10                  15      


Ala Ile Ala Asn Thr Leu Val Tyr Pro Leu Asp Leu Ser Lys Thr Leu 
            20                  25                  30          


Ile Gln Thr Gln Val Lys Pro Arg Ala Ile Gly Met Asn Pro Ser Asp 
        35                  40                  45              


Ser Thr Ala Ser Ile Ala Ser Asp Thr Ser Asp Pro Glu Glu Ser Val 
    50                  55                  60                  


Trp His Gln Lys Gln Gly Lys Asp Gly Gln Leu Arg Tyr Lys Ser Thr 
65                  70                  75                  80  


Val Asp Ala Leu Ala Gln Ile Tyr Lys Lys Lys Gly Ile Leu Gly Trp 
                85                  90                  95      


Tyr His Gly Leu Ile Ser Ser Ile Leu Gly Ser Ala Ala Gln Asn Phe 
            100                 105                 110         


Ser Tyr Phe Tyr Trp Tyr Thr Ile Val Lys Arg Val Tyr Ala Arg Ile 
        115                 120                 125             


Asn Lys His Ile Pro Asn His Arg Pro Gly Thr Ala Thr Glu Leu Phe 
    130                 135                 140                 


Leu Gly Ala Leu Ala Ala Ala Ile Ser Gln Leu Phe Thr Met Pro Ile 
145                 150                 155                 160 


Gly Val Ile Thr Thr His Gln Gln Thr Asp Lys His His Lys Gly Leu 
                165                 170                 175     


Val Gln Leu Thr Arg Glu Ile Leu Glu His Asp Gly Ile Ser Gly Leu 
            180                 185                 190         


Trp Lys Gly Leu Arg Val Ser Leu Val Leu Cys Ile Asn Pro Ser Ile 
        195                 200                 205             


Thr Tyr Gly Ser Tyr Glu Arg Leu Lys Gln Ile Ile Tyr Ala Asn Lys 
    210                 215                 220                 


Glu Tyr Leu Gly Pro Leu Glu Ser Phe Ser Ile Gly Val Ile Ala Lys 
225                 230                 235                 240 


Ser Met Ala Thr Leu Ala Thr Gln Pro Leu Ile Val Ser Lys Ala Met 
                245                 250                 255     


Leu Gln Lys Lys Ser His Val Lys Pro Gly Glu Ile Asp Asp Gly Asp 
            260                 265                 270         


Val Lys Phe Asp Asn Phe Gln Asp Ala Leu Ala His Leu Trp His Thr 
        275                 280                 285             


Glu Lys Leu Ala Gly Leu Tyr Lys Gly Ile Ala Pro Gln Leu Val Lys 
    290                 295                 300                 


Gly Val Phe Val Gln Gly Leu Leu Phe Met Phe Lys Asp Gln Ile Asp 
305                 310                 315                 320 


Leu Val Phe Leu Ser Leu Leu Lys Ile Ala Lys Lys Lys 
                325                 330             


<210>  6
<211>  328
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  6

Met Leu Thr Leu Glu Ser Ala Leu Thr Gly Ala Val Ala Ser Ala Met 
1               5                   10                  15      


Ala Asn Ile Ala Val Tyr Pro Leu Asp Leu Ser Lys Thr Ile Ile Gln 
            20                  25                  30          


Ser Gln Val Ser Pro Ser Ser Ser Glu Asp Ser Asn Glu Gly Lys Val 
        35                  40                  45              


Leu Pro Asn Arg Arg Tyr Lys Asn Val Val Asp Cys Met Ile Asn Ile 
    50                  55                  60                  


Phe Lys Glu Lys Gly Ile Leu Gly Leu Tyr Gln Gly Met Thr Val Thr 
65                  70                  75                  80  


Thr Val Ala Thr Phe Val Gln Asn Phe Val Tyr Phe Phe Trp Tyr Thr 
                85                  90                  95      


Phe Ile Arg Lys Ser Tyr Met Lys His Lys Leu Leu Gly Leu Gln Ser 
            100                 105                 110         


Leu Lys Asn Arg Asp Gly Pro Ile Thr Pro Ser Thr Ile Glu Glu Leu 
        115                 120                 125             


Val Leu Gly Val Ala Ala Ala Ser Ile Ser Gln Leu Phe Thr Ser Pro 
    130                 135                 140                 


Met Ala Val Val Ala Thr Arg Gln Gln Thr Val His Ser Ala Glu Ser 
145                 150                 155                 160 


Ala Lys Phe Thr Asn Val Ile Lys Asp Ile Tyr Arg Glu Asn Asn Gly 
                165                 170                 175     


Asp Ile Thr Ala Phe Trp Lys Gly Leu Arg Thr Gly Leu Ala Leu Thr 
            180                 185                 190         


Ile Asn Pro Ser Ile Thr Tyr Ala Ser Phe Gln Arg Leu Lys Glu Val 
        195                 200                 205             


Phe Phe His Asp His Ser Asn Asp Ala Gly Ser Leu Ser Ala Val Gln 
    210                 215                 220                 


Asn Phe Ile Leu Gly Val Leu Ser Lys Met Ile Ser Thr Leu Val Thr 
225                 230                 235                 240 


Gln Pro Leu Ile Val Ala Lys Ala Met Leu Gln Ser Ala Gly Ser Lys 
                245                 250                 255     


Phe Thr Thr Phe Gln Glu Ala Leu Leu Tyr Leu Tyr Lys Asn Glu Gly 
            260                 265                 270         


Leu Lys Ser Leu Trp Lys Gly Val Leu Pro Gln Leu Thr Lys Gly Val 
        275                 280                 285             


Ile Val Gln Gly Leu Leu Phe Ala Phe Arg Gly Glu Leu Thr Lys Ser 
    290                 295                 300                 


Leu Lys Arg Leu Ile Phe Leu Tyr Ser Ser Phe Phe Leu Lys His Asn 
305                 310                 315                 320 


Gly Gln Arg Lys Leu Ala Ser Thr 
                325             


<210>  7
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide

<400>  7
gataagcttg atatcgaatt cctgcagccc gcggccgcgg agagggtaaa gccgacaac        59


<210>  8
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide

<400>  8
cgataattac cctgttatcc ctagcccggg tatgccttac cgagaccgcc ac               52


<210>  9
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide

<400>  9
cccgggctag ggataacagg gta                                               23


<210>  10
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide

<400>  10
atcgatgatt accctgttat ccctacataa c                                      31


<210>  11
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide

<400>  11
gttatgtagg gataacaggg taatcatcga tagaccaagg ttgttgagcg agg              53


<210>  12
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide

<400>  12
ggcggccgct ctagaactag tggatccccc gttcagtttc gtcgccatgt c                51


<210>  13
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide

<400>  13
actgggtacc ggatcccaat attacacc                                          28


<210>  14
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide

<400>  14
actgggtacc gatccagtct acactgat                                          28


<210>  15
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide

<400>  15
aagctggagc tccaccgcgg tggcggccgc gtgactactt gtagaacgtg tgtgg            55


<210>  16
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide

<400>  16
ccgaagcgat aattaccctg ttatccctag cccgggctgt gtgtagtgtg tgttgtcgaa       60

a                                                                       61


<210>  17
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide

<400>  17
acgaagttat gtagggataa cagggtaatc atcgataagg gtgcgaagat tacgacga         58


<210>  18
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide

<400>  18
cgggggatcc actagttcta gagcggccgc ttctcttact gcgctctgcc                  50


<210>  19
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide

<400>  19
cggtgccggt ggcggtctcg gtaaggcata cccgggggta ccggttgaaa tg               52


<210>  20
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide

<400>  20
aactaatttc taactaaatc caactaatgc tagcatcgga tgccagga                    48


<210>  21
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide

<400>  21
gacttaagtg cacaactcct ggcatccgat gctagcatta gttggattta gttagaaatt       60

agttgac                                                                 67


<210>  22
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide

<400>  22
aagcgataat taccctgtta tccctagccc gggaattgtg gacttcacca a                51


