                         LISTADO DE SECUENCIAS

<110>  ABENGOA BIOENERGA NUEVAS TECNOLOGAS, S.A.
 
<120>  "VARIANTES MEJORADAS DE CELOBIOHIDROLASA 1"

<130>  PCT1861.44

<160>  19    

<170>  PatentIn version 3.5

<210>  1
<211>  1648
<212>  DNA
<213>  Myceliophthora thermophila

<400>  1
atgtacgcca agttcgcgac cctcgccgcc cttgtggctg gcgccgctgc tcagaacgcc       60

tgcactctga ccgctgagaa ccacccctcg ctgacgtggt ccaagtgcac gtctggcggc      120

agctgcacca gcgtccaggg ttccatcacc atcgacgcca actggcggtg gactcaccgg      180

accgatagcg ccaccaactg ctacgagggc aacaagtggg atacttcgta ctgcagcgat      240

ggtccttctt gcgcctccaa gtgctgcatc gacggcgctg actactcgag cacctatggc      300

atcaccacga gcggtaactc cctgaacctc aagttcgtca ccaagggcca gtactcgacc      360

aacatcggct cgcgtaccta cctgatggag agcgacacca agtaccagag taagttcctc      420

tcgcacccgg ccgccgggag atgatggcgc ccagcccgct gacgcgaatg acacagtgtt      480

ccagctcctc ggcaacgagt tcaccttcga tgtcgacgtc tccaacctcg gctgcggcct      540

caatggcgcc ctctacttcg tgtccatgga tgccgatggt ggcatgtcca agtactcggg      600

caacaaggca ggtgccaagt acggtaccgg ctactgtgat tctcagtgcc cccgcgacct      660

caagttcatc aacggcgagg ccaacgtaga gaactggcag agctcgacca acgatgccaa      720

cgccggcacg ggcaagtacg gcagctgctg ctccgagatg gacgtctggg aggccaacaa      780

catggccgcc gccttcactc cccacccttg caccgtgatc ggccagtcgc gctgcgaggg      840

cgactcgtgc ggcggtacct acagcaccga ccgctatgcc ggcatctgcg accccgacgg      900

atgcgacttc aactcgtacc gccagggcaa caagaccttc tacggcaagg gcatgacggt      960

cgacacgacc aagaagatca cggtcgtcac ccagttcctc aagaactcgg ccggcgagct     1020

ctccgagatc aagcggttct acgtccagaa cggcaaggtc atccccaact ccgagtccac     1080

catcccgggc gtcgagggca actccatcac ccaggactgg tgcgaccgcc agaaggccgc     1140

cttcggcgac gtgaccgact tccaggacaa gggcggcatg gtccagatgg gcaaggccct     1200

cgcggggccc atggtcctcg tcatgtccat ctgggacgac cacgccgtca acatgctctg     1260

gctcgactcc acctggccca tcgacggcgc cggcaagccg ggcgccgagc gcggtgcctg     1320

ccccaccacc tcgggcgtcc ccgctgaggt cgaggccgag gcccccaact ccaacgtcat     1380

cttctccaac atccgcttcg gccccatcgg ctccaccgtc tccggcctgc ccgacggcgg     1440

cagcggcaac cccaacccgc ccgtcagctc gtccaccccg gtcccctcct cgtccaccac     1500

atcctccggt tcctccggcc cgactggcgg cacgggtgtc gctaagcact atgagcaatg     1560

cggaggaatc gggttcactg gccctaccca gtgcgagagc ccctacactt gcaccaagct     1620

gaatgactgg tactcgcagt gcctgtaa                                        1648


<210>  2
<211>  526
<212>  PRT
<213>  Myceliophthora thermophila


<220>
<221>  SIGNAL
<222>  (1)..(17)

<400>  2

Met Tyr Ala Lys Phe Ala Thr Leu Ala Ala Leu Val Ala Gly Ala Ala 
1               5                   10                  15      


Ala Gln Asn Ala Cys Thr Leu Thr Ala Glu Asn His Pro Ser Leu Thr 
            20                  25                  30          


Trp Ser Lys Cys Thr Ser Gly Gly Ser Cys Thr Ser Val Gln Gly Ser 
        35                  40                  45              


Ile Thr Ile Asp Ala Asn Trp Arg Trp Thr His Arg Thr Asp Ser Ala 
    50                  55                  60                  


Thr Asn Cys Tyr Glu Gly Asn Lys Trp Asp Thr Ser Tyr Cys Ser Asp 
65                  70                  75                  80  


Gly Pro Ser Cys Ala Ser Lys Cys Cys Ile Asp Gly Ala Asp Tyr Ser 
                85                  90                  95      


Ser Thr Tyr Gly Ile Thr Thr Ser Gly Asn Ser Leu Asn Leu Lys Phe 
            100                 105                 110         


Val Thr Lys Gly Gln Tyr Ser Thr Asn Ile Gly Ser Arg Thr Tyr Leu 
        115                 120                 125             


Met Glu Ser Asp Thr Lys Tyr Gln Met Phe Gln Leu Leu Gly Asn Glu 
    130                 135                 140                 


Phe Thr Phe Asp Val Asp Val Ser Asn Leu Gly Cys Gly Leu Asn Gly 
145                 150                 155                 160 


Ala Leu Tyr Phe Val Ser Met Asp Ala Asp Gly Gly Met Ser Lys Tyr 
                165                 170                 175     


Ser Gly Asn Lys Ala Gly Ala Lys Tyr Gly Thr Gly Tyr Cys Asp Ser 
            180                 185                 190         


Gln Cys Pro Arg Asp Leu Lys Phe Ile Asn Gly Glu Ala Asn Val Glu 
        195                 200                 205             


Asn Trp Gln Ser Ser Thr Asn Asp Ala Asn Ala Gly Thr Gly Lys Tyr 
    210                 215                 220                 


Gly Ser Cys Cys Ser Glu Met Asp Val Trp Glu Ala Asn Asn Met Ala 
225                 230                 235                 240 


Ala Ala Phe Thr Pro His Pro Cys Thr Val Ile Gly Gln Ser Arg Cys 
                245                 250                 255     


Glu Gly Asp Ser Cys Gly Gly Thr Tyr Ser Thr Asp Arg Tyr Ala Gly 
            260                 265                 270         


Ile Cys Asp Pro Asp Gly Cys Asp Phe Asn Ser Tyr Arg Gln Gly Asn 
        275                 280                 285             


Lys Thr Phe Tyr Gly Lys Gly Met Thr Val Asp Thr Thr Lys Lys Ile 
    290                 295                 300                 


Thr Val Val Thr Gln Phe Leu Lys Asn Ser Ala Gly Glu Leu Ser Glu 
305                 310                 315                 320 


Ile Lys Arg Phe Tyr Val Gln Asn Gly Lys Val Ile Pro Asn Ser Glu 
                325                 330                 335     


Ser Thr Ile Pro Gly Val Glu Gly Asn Ser Ile Thr Gln Asp Trp Cys 
            340                 345                 350         


Asp Arg Gln Lys Ala Ala Phe Gly Asp Val Thr Asp Phe Gln Asp Lys 
        355                 360                 365             


Gly Gly Met Val Gln Met Gly Lys Ala Leu Ala Gly Pro Met Val Leu 
    370                 375                 380                 


Val Met Ser Ile Trp Asp Asp His Ala Val Asn Met Leu Trp Leu Asp 
385                 390                 395                 400 


Ser Thr Trp Pro Ile Asp Gly Ala Gly Lys Pro Gly Ala Glu Arg Gly 
                405                 410                 415     


Ala Cys Pro Thr Thr Ser Gly Val Pro Ala Glu Val Glu Ala Glu Ala 
            420                 425                 430         


Pro Asn Ser Asn Val Ile Phe Ser Asn Ile Arg Phe Gly Pro Ile Gly 
        435                 440                 445             


Ser Thr Val Ser Gly Leu Pro Asp Gly Gly Ser Gly Asn Pro Asn Pro 
    450                 455                 460                 


Pro Val Ser Ser Ser Thr Pro Val Pro Ser Ser Ser Thr Thr Ser Ser 
465                 470                 475                 480 


Gly Ser Ser Gly Pro Thr Gly Gly Thr Gly Val Ala Lys His Tyr Glu 
                485                 490                 495     


Gln Cys Gly Gly Ile Gly Phe Thr Gly Pro Thr Gln Cys Glu Ser Pro 
            500                 505                 510         


Tyr Thr Cys Thr Lys Leu Asn Asp Trp Tyr Ser Gln Cys Leu 
        515                 520                 525     


<210>  3
<211>  509
<212>  PRT
<213>  Myceliophthora thermophila

<400>  3

Gln Asn Ala Cys Thr Leu Thr Ala Glu Asn His Pro Ser Leu Thr Trp 
1               5                   10                  15      


Ser Lys Cys Thr Ser Gly Gly Ser Cys Thr Ser Val Gln Gly Ser Ile 
            20                  25                  30          


Thr Ile Asp Ala Asn Trp Arg Trp Thr His Arg Thr Asp Ser Ala Thr 
        35                  40                  45              


Asn Cys Tyr Glu Gly Asn Lys Trp Asp Thr Ser Tyr Cys Ser Asp Gly 
    50                  55                  60                  


Pro Ser Cys Ala Ser Lys Cys Cys Ile Asp Gly Ala Asp Tyr Ser Ser 
65                  70                  75                  80  


Thr Tyr Gly Ile Thr Thr Ser Gly Asn Ser Leu Asn Leu Lys Phe Val 
                85                  90                  95      


Thr Lys Gly Gln Tyr Ser Thr Asn Ile Gly Ser Arg Thr Tyr Leu Met 
            100                 105                 110         


Glu Ser Asp Thr Lys Tyr Gln Met Phe Gln Leu Leu Gly Asn Glu Phe 
        115                 120                 125             


Thr Phe Asp Val Asp Val Ser Asn Leu Gly Cys Gly Leu Asn Gly Ala 
    130                 135                 140                 


Leu Tyr Phe Val Ser Met Asp Ala Asp Gly Gly Met Ser Lys Tyr Ser 
145                 150                 155                 160 


Gly Asn Lys Ala Gly Ala Lys Tyr Gly Thr Gly Tyr Cys Asp Ser Gln 
                165                 170                 175     


Cys Pro Arg Asp Leu Lys Phe Ile Asn Gly Glu Ala Asn Val Glu Asn 
            180                 185                 190         


Trp Gln Ser Ser Thr Asn Asp Ala Asn Ala Gly Thr Gly Lys Tyr Gly 
        195                 200                 205             


Ser Cys Cys Ser Glu Met Asp Val Trp Glu Ala Asn Asn Met Ala Ala 
    210                 215                 220                 


Ala Phe Thr Pro His Pro Cys Thr Val Ile Gly Gln Ser Arg Cys Glu 
225                 230                 235                 240 


Gly Asp Ser Cys Gly Gly Thr Tyr Ser Thr Asp Arg Tyr Ala Gly Ile 
                245                 250                 255     


Cys Asp Pro Asp Gly Cys Asp Phe Asn Ser Tyr Arg Gln Gly Asn Lys 
            260                 265                 270         


Thr Phe Tyr Gly Lys Gly Met Thr Val Asp Thr Thr Lys Lys Ile Thr 
        275                 280                 285             


Val Val Thr Gln Phe Leu Lys Asn Ser Ala Gly Glu Leu Ser Glu Ile 
    290                 295                 300                 


Lys Arg Phe Tyr Val Gln Asn Gly Lys Val Ile Pro Asn Ser Glu Ser 
305                 310                 315                 320 


Thr Ile Pro Gly Val Glu Gly Asn Ser Ile Thr Gln Asp Trp Cys Asp 
                325                 330                 335     


Arg Gln Lys Ala Ala Phe Gly Asp Val Thr Asp Phe Gln Asp Lys Gly 
            340                 345                 350         


Gly Met Val Gln Met Gly Lys Ala Leu Ala Gly Pro Met Val Leu Val 
        355                 360                 365             


Met Ser Ile Trp Asp Asp His Ala Val Asn Met Leu Trp Leu Asp Ser 
    370                 375                 380                 


Thr Trp Pro Ile Asp Gly Ala Gly Lys Pro Gly Ala Glu Arg Gly Ala 
385                 390                 395                 400 


Cys Pro Thr Thr Ser Gly Val Pro Ala Glu Val Glu Ala Glu Ala Pro 
                405                 410                 415     


Asn Ser Asn Val Ile Phe Ser Asn Ile Arg Phe Gly Pro Ile Gly Ser 
            420                 425                 430         


Thr Val Ser Gly Leu Pro Asp Gly Gly Ser Gly Asn Pro Asn Pro Pro 
        435                 440                 445             


Val Ser Ser Ser Thr Pro Val Pro Ser Ser Ser Thr Thr Ser Ser Gly 
    450                 455                 460                 


Ser Ser Gly Pro Thr Gly Gly Thr Gly Val Ala Lys His Tyr Glu Gln 
465                 470                 475                 480 


Cys Gly Gly Ile Gly Phe Thr Gly Pro Thr Gln Cys Glu Ser Pro Tyr 
                485                 490                 495     


Thr Cys Thr Lys Leu Asn Asp Trp Tyr Ser Gln Cys Leu 
            500                 505                 


<210>  4
<211>  1648
<212>  DNA
<213>  Secuencia Artificial

<220>
<223>  Polinucletido que codifica para la preprotena mutante Cbh1N209D
       de SEQ ID NO: 5

<400>  4
atgtacgcca agttcgcgac cctcgccgcc cttgtggctg gcgccgctgc tcagaacgcc       60

tgcactctga ccgctgagaa ccacccctcg ctgacgtggt ccaagtgcac gtctggcggc      120

agctgcacca gcgtccaggg ttccatcacc atcgacgcca actggcggtg gactcaccgg      180

accgatagcg ccaccaactg ctacgagggc aacaagtggg atacttcgta ctgcagcgat      240

ggtccttctt gcgcctccaa gtgctgcatc gacggcgctg actactcgag cacctatggc      300

atcaccacga gcggtaactc cctgaacctc aagttcgtca ccaagggcca gtactcgacc      360

aacatcggct cgcgtaccta cctgatggag agcgacacca agtaccagag taagttcctc      420

tcgcacccgg ccgccgggag atgatggcgc ccagcccgct gacgcgaatg acacagtgtt      480

ccagctcctc ggcaacgagt tcaccttcga tgtcgacgtc tccaacctcg gctgcggcct      540

caatggcgcc ctctacttcg tgtccatgga tgccgatggt ggcatgtcca agtactcggg      600

caacaaggca ggtgccaagt acggtaccgg ctactgtgat tctcagtgcc cccgcgacct      660

caagttcatc aacggcgagg ccaacgtaga ggactggcag agctcgacca acgatgccaa      720

cgccggcacg ggcaagtacg gcagctgctg ctccgagatg gacgtctggg aggccaacaa      780

catggccgcc gccttcactc cccacccttg caccgtgatc ggccagtcgc gctgcgaggg      840

cgactcgtgc ggcggtacct acagcaccga ccgctatgcc ggcatctgcg accccgacgg      900

atgcgacttc aactcgtacc gccagggcaa caagaccttc tacggcaagg gcatgacggt      960

cgacacgacc aagaagatca cggtcgtcac ccagttcctc aagaactcgg ccggcgagct     1020

ctccgagatc aagcggttct acgtccagaa cggcaaggtc atccccaact ccgagtccac     1080

catcccgggc gtcgagggca actccatcac ccaggactgg tgcgaccgcc agaaggccgc     1140

cttcggcgac gtgaccgact tccaggacaa gggcggcatg gtccagatgg gcaaggccct     1200

cgcggggccc atggtcctcg tcatgtccat ctgggacgac cacgccgtca acatgctctg     1260

gctcgactcc acctggccca tcgacggcgc cggcaagccg ggcgccgagc gcggtgcctg     1320

ccccaccacc tcgggcgtcc ccgctgaggt cgaggccgag gcccccaact ccaacgtcat     1380

cttctccaac atccgcttcg gccccatcgg ctccaccgtc tccggcctgc ccgacggcgg     1440

cagcggcaac cccaacccgc ccgtcagctc gtccaccccg gtcccctcct cgtccaccac     1500

atcctccggt tcctccggcc cgactggcgg cacgggtgtc gctaagcact atgagcaatg     1560

cggaggaatc gggttcactg gccctaccca gtgcgagagc ccctacactt gcaccaagct     1620

gaatgactgg tactcgcagt gcctgtaa                                        1648


<210>  5
<211>  526
<212>  PRT
<213>  Secuencia Artificial

<220>
<223>  Preprotena de la Cbh1 mutante Cbh1N209D


<220>
<221>  SIGNAL
<222>  (1)..(17)

<400>  5

Met Tyr Ala Lys Phe Ala Thr Leu Ala Ala Leu Val Ala Gly Ala Ala 
1               5                   10                  15      


Ala Gln Asn Ala Cys Thr Leu Thr Ala Glu Asn His Pro Ser Leu Thr 
            20                  25                  30          


Trp Ser Lys Cys Thr Ser Gly Gly Ser Cys Thr Ser Val Gln Gly Ser 
        35                  40                  45              


Ile Thr Ile Asp Ala Asn Trp Arg Trp Thr His Arg Thr Asp Ser Ala 
    50                  55                  60                  


Thr Asn Cys Tyr Glu Gly Asn Lys Trp Asp Thr Ser Tyr Cys Ser Asp 
65                  70                  75                  80  


Gly Pro Ser Cys Ala Ser Lys Cys Cys Ile Asp Gly Ala Asp Tyr Ser 
                85                  90                  95      


Ser Thr Tyr Gly Ile Thr Thr Ser Gly Asn Ser Leu Asn Leu Lys Phe 
            100                 105                 110         


Val Thr Lys Gly Gln Tyr Ser Thr Asn Ile Gly Ser Arg Thr Tyr Leu 
        115                 120                 125             


Met Glu Ser Asp Thr Lys Tyr Gln Met Phe Gln Leu Leu Gly Asn Glu 
    130                 135                 140                 


Phe Thr Phe Asp Val Asp Val Ser Asn Leu Gly Cys Gly Leu Asn Gly 
145                 150                 155                 160 


Ala Leu Tyr Phe Val Ser Met Asp Ala Asp Gly Gly Met Ser Lys Tyr 
                165                 170                 175     


Ser Gly Asn Lys Ala Gly Ala Lys Tyr Gly Thr Gly Tyr Cys Asp Ser 
            180                 185                 190         


Gln Cys Pro Arg Asp Leu Lys Phe Ile Asn Gly Glu Ala Asn Val Glu 
        195                 200                 205             


Asp Trp Gln Ser Ser Thr Asn Asp Ala Asn Ala Gly Thr Gly Lys Tyr 
    210                 215                 220                 


Gly Ser Cys Cys Ser Glu Met Asp Val Trp Glu Ala Asn Asn Met Ala 
225                 230                 235                 240 


Ala Ala Phe Thr Pro His Pro Cys Thr Val Ile Gly Gln Ser Arg Cys 
                245                 250                 255     


Glu Gly Asp Ser Cys Gly Gly Thr Tyr Ser Thr Asp Arg Tyr Ala Gly 
            260                 265                 270         


Ile Cys Asp Pro Asp Gly Cys Asp Phe Asn Ser Tyr Arg Gln Gly Asn 
        275                 280                 285             


Lys Thr Phe Tyr Gly Lys Gly Met Thr Val Asp Thr Thr Lys Lys Ile 
    290                 295                 300                 


Thr Val Val Thr Gln Phe Leu Lys Asn Ser Ala Gly Glu Leu Ser Glu 
305                 310                 315                 320 


Ile Lys Arg Phe Tyr Val Gln Asn Gly Lys Val Ile Pro Asn Ser Glu 
                325                 330                 335     


Ser Thr Ile Pro Gly Val Glu Gly Asn Ser Ile Thr Gln Asp Trp Cys 
            340                 345                 350         


Asp Arg Gln Lys Ala Ala Phe Gly Asp Val Thr Asp Phe Gln Asp Lys 
        355                 360                 365             


Gly Gly Met Val Gln Met Gly Lys Ala Leu Ala Gly Pro Met Val Leu 
    370                 375                 380                 


Val Met Ser Ile Trp Asp Asp His Ala Val Asn Met Leu Trp Leu Asp 
385                 390                 395                 400 


Ser Thr Trp Pro Ile Asp Gly Ala Gly Lys Pro Gly Ala Glu Arg Gly 
                405                 410                 415     


Ala Cys Pro Thr Thr Ser Gly Val Pro Ala Glu Val Glu Ala Glu Ala 
            420                 425                 430         


Pro Asn Ser Asn Val Ile Phe Ser Asn Ile Arg Phe Gly Pro Ile Gly 
        435                 440                 445             


Ser Thr Val Ser Gly Leu Pro Asp Gly Gly Ser Gly Asn Pro Asn Pro 
    450                 455                 460                 


Pro Val Ser Ser Ser Thr Pro Val Pro Ser Ser Ser Thr Thr Ser Ser 
465                 470                 475                 480 


Gly Ser Ser Gly Pro Thr Gly Gly Thr Gly Val Ala Lys His Tyr Glu 
                485                 490                 495     


Gln Cys Gly Gly Ile Gly Phe Thr Gly Pro Thr Gln Cys Glu Ser Pro 
            500                 505                 510         


Tyr Thr Cys Thr Lys Leu Asn Asp Trp Tyr Ser Gln Cys Leu 
        515                 520                 525     


<210>  6
<211>  509
<212>  PRT
<213>  Secuencia Artificial

<220>
<223>  Protena madura Cbh1N209D

<400>  6

Gln Asn Ala Cys Thr Leu Thr Ala Glu Asn His Pro Ser Leu Thr Trp 
1               5                   10                  15      


Ser Lys Cys Thr Ser Gly Gly Ser Cys Thr Ser Val Gln Gly Ser Ile 
            20                  25                  30          


Thr Ile Asp Ala Asn Trp Arg Trp Thr His Arg Thr Asp Ser Ala Thr 
        35                  40                  45              


Asn Cys Tyr Glu Gly Asn Lys Trp Asp Thr Ser Tyr Cys Ser Asp Gly 
    50                  55                  60                  


Pro Ser Cys Ala Ser Lys Cys Cys Ile Asp Gly Ala Asp Tyr Ser Ser 
65                  70                  75                  80  


Thr Tyr Gly Ile Thr Thr Ser Gly Asn Ser Leu Asn Leu Lys Phe Val 
                85                  90                  95      


Thr Lys Gly Gln Tyr Ser Thr Asn Ile Gly Ser Arg Thr Tyr Leu Met 
            100                 105                 110         


Glu Ser Asp Thr Lys Tyr Gln Met Phe Gln Leu Leu Gly Asn Glu Phe 
        115                 120                 125             


Thr Phe Asp Val Asp Val Ser Asn Leu Gly Cys Gly Leu Asn Gly Ala 
    130                 135                 140                 


Leu Tyr Phe Val Ser Met Asp Ala Asp Gly Gly Met Ser Lys Tyr Ser 
145                 150                 155                 160 


Gly Asn Lys Ala Gly Ala Lys Tyr Gly Thr Gly Tyr Cys Asp Ser Gln 
                165                 170                 175     


Cys Pro Arg Asp Leu Lys Phe Ile Asn Gly Glu Ala Asn Val Glu Asp 
            180                 185                 190         


Trp Gln Ser Ser Thr Asn Asp Ala Asn Ala Gly Thr Gly Lys Tyr Gly 
        195                 200                 205             


Ser Cys Cys Ser Glu Met Asp Val Trp Glu Ala Asn Asn Met Ala Ala 
    210                 215                 220                 


Ala Phe Thr Pro His Pro Cys Thr Val Ile Gly Gln Ser Arg Cys Glu 
225                 230                 235                 240 


Gly Asp Ser Cys Gly Gly Thr Tyr Ser Thr Asp Arg Tyr Ala Gly Ile 
                245                 250                 255     


Cys Asp Pro Asp Gly Cys Asp Phe Asn Ser Tyr Arg Gln Gly Asn Lys 
            260                 265                 270         


Thr Phe Tyr Gly Lys Gly Met Thr Val Asp Thr Thr Lys Lys Ile Thr 
        275                 280                 285             


Val Val Thr Gln Phe Leu Lys Asn Ser Ala Gly Glu Leu Ser Glu Ile 
    290                 295                 300                 


Lys Arg Phe Tyr Val Gln Asn Gly Lys Val Ile Pro Asn Ser Glu Ser 
305                 310                 315                 320 


Thr Ile Pro Gly Val Glu Gly Asn Ser Ile Thr Gln Asp Trp Cys Asp 
                325                 330                 335     


Arg Gln Lys Ala Ala Phe Gly Asp Val Thr Asp Phe Gln Asp Lys Gly 
            340                 345                 350         


Gly Met Val Gln Met Gly Lys Ala Leu Ala Gly Pro Met Val Leu Val 
        355                 360                 365             


Met Ser Ile Trp Asp Asp His Ala Val Asn Met Leu Trp Leu Asp Ser 
    370                 375                 380                 


Thr Trp Pro Ile Asp Gly Ala Gly Lys Pro Gly Ala Glu Arg Gly Ala 
385                 390                 395                 400 


Cys Pro Thr Thr Ser Gly Val Pro Ala Glu Val Glu Ala Glu Ala Pro 
                405                 410                 415     


Asn Ser Asn Val Ile Phe Ser Asn Ile Arg Phe Gly Pro Ile Gly Ser 
            420                 425                 430         


Thr Val Ser Gly Leu Pro Asp Gly Gly Ser Gly Asn Pro Asn Pro Pro 
        435                 440                 445             


Val Ser Ser Ser Thr Pro Val Pro Ser Ser Ser Thr Thr Ser Ser Gly 
    450                 455                 460                 


Ser Ser Gly Pro Thr Gly Gly Thr Gly Val Ala Lys His Tyr Glu Gln 
465                 470                 475                 480 


Cys Gly Gly Ile Gly Phe Thr Gly Pro Thr Gln Cys Glu Ser Pro Tyr 
                485                 490                 495     


Thr Cys Thr Lys Leu Asn Asp Trp Tyr Ser Gln Cys Leu 
            500                 505                 


<210>  7
<211>  1648
<212>  DNA
<213>  Secuencia Artificial

<220>
<223>  Polinucletido que codifica para la preprotena mutante Cbh1N209E
       de SEQ ID NO: 8

<400>  7
atgtacgcca agttcgcgac cctcgccgcc cttgtggctg gcgccgctgc tcagaacgcc       60

tgcactctga ccgctgagaa ccacccctcg ctgacgtggt ccaagtgcac gtctggcggc      120

agctgcacca gcgtccaggg ttccatcacc atcgacgcca actggcggtg gactcaccgg      180

accgatagcg ccaccaactg ctacgagggc aacaagtggg atacttcgta ctgcagcgat      240

ggtccttctt gcgcctccaa gtgctgcatc gacggcgctg actactcgag cacctatggc      300

atcaccacga gcggtaactc cctgaacctc aagttcgtca ccaagggcca gtactcgacc      360

aacatcggct cgcgtaccta cctgatggag agcgacacca agtaccagag taagttcctc      420

tcgcacccgg ccgccgggag atgatggcgc ccagcccgct gacgcgaatg acacagtgtt      480

ccagctcctc ggcaacgagt tcaccttcga tgtcgacgtc tccaacctcg gctgcggcct      540

caatggcgcc ctctacttcg tgtccatgga tgccgatggt ggcatgtcca agtactcggg      600

caacaaggca ggtgccaagt acggtaccgg ctactgtgat tctcagtgcc cccgcgacct      660

caagttcatc aacggcgagg ccaacgtaga ggagtggcag agctcgacca acgatgccaa      720

cgccggcacg ggcaagtacg gcagctgctg ctccgagatg gacgtctggg aggccaacaa      780

catggccgcc gccttcactc cccacccttg caccgtgatc ggccagtcgc gctgcgaggg      840

cgactcgtgc ggcggtacct acagcaccga ccgctatgcc ggcatctgcg accccgacgg      900

atgcgacttc aactcgtacc gccagggcaa caagaccttc tacggcaagg gcatgacggt      960

cgacacgacc aagaagatca cggtcgtcac ccagttcctc aagaactcgg ccggcgagct     1020

ctccgagatc aagcggttct acgtccagaa cggcaaggtc atccccaact ccgagtccac     1080

catcccgggc gtcgagggca actccatcac ccaggactgg tgcgaccgcc agaaggccgc     1140

cttcggcgac gtgaccgact tccaggacaa gggcggcatg gtccagatgg gcaaggccct     1200

cgcggggccc atggtcctcg tcatgtccat ctgggacgac cacgccgtca acatgctctg     1260

gctcgactcc acctggccca tcgacggcgc cggcaagccg ggcgccgagc gcggtgcctg     1320

ccccaccacc tcgggcgtcc ccgctgaggt cgaggccgag gcccccaact ccaacgtcat     1380

cttctccaac atccgcttcg gccccatcgg ctccaccgtc tccggcctgc ccgacggcgg     1440

cagcggcaac cccaacccgc ccgtcagctc gtccaccccg gtcccctcct cgtccaccac     1500

atcctccggt tcctccggcc cgactggcgg cacgggtgtc gctaagcact atgagcaatg     1560

cggaggaatc gggttcactg gccctaccca gtgcgagagc ccctacactt gcaccaagct     1620

gaatgactgg tactcgcagt gcctgtaa                                        1648


<210>  8
<211>  526
<212>  PRT
<213>  Secuencia Artificial

<220>
<223>  Preprotena de la Cbh1 mutante Cbh1N209E


<220>
<221>  SIGNAL
<222>  (1)..(17)

<400>  8

Met Tyr Ala Lys Phe Ala Thr Leu Ala Ala Leu Val Ala Gly Ala Ala 
1               5                   10                  15      


Ala Gln Asn Ala Cys Thr Leu Thr Ala Glu Asn His Pro Ser Leu Thr 
            20                  25                  30          


Trp Ser Lys Cys Thr Ser Gly Gly Ser Cys Thr Ser Val Gln Gly Ser 
        35                  40                  45              


Ile Thr Ile Asp Ala Asn Trp Arg Trp Thr His Arg Thr Asp Ser Ala 
    50                  55                  60                  


Thr Asn Cys Tyr Glu Gly Asn Lys Trp Asp Thr Ser Tyr Cys Ser Asp 
65                  70                  75                  80  


Gly Pro Ser Cys Ala Ser Lys Cys Cys Ile Asp Gly Ala Asp Tyr Ser 
                85                  90                  95      


Ser Thr Tyr Gly Ile Thr Thr Ser Gly Asn Ser Leu Asn Leu Lys Phe 
            100                 105                 110         


Val Thr Lys Gly Gln Tyr Ser Thr Asn Ile Gly Ser Arg Thr Tyr Leu 
        115                 120                 125             


Met Glu Ser Asp Thr Lys Tyr Gln Met Phe Gln Leu Leu Gly Asn Glu 
    130                 135                 140                 


Phe Thr Phe Asp Val Asp Val Ser Asn Leu Gly Cys Gly Leu Asn Gly 
145                 150                 155                 160 


Ala Leu Tyr Phe Val Ser Met Asp Ala Asp Gly Gly Met Ser Lys Tyr 
                165                 170                 175     


Ser Gly Asn Lys Ala Gly Ala Lys Tyr Gly Thr Gly Tyr Cys Asp Ser 
            180                 185                 190         


Gln Cys Pro Arg Asp Leu Lys Phe Ile Asn Gly Glu Ala Asn Val Glu 
        195                 200                 205             


Glu Trp Gln Ser Ser Thr Asn Asp Ala Asn Ala Gly Thr Gly Lys Tyr 
    210                 215                 220                 


Gly Ser Cys Cys Ser Glu Met Asp Val Trp Glu Ala Asn Asn Met Ala 
225                 230                 235                 240 


Ala Ala Phe Thr Pro His Pro Cys Thr Val Ile Gly Gln Ser Arg Cys 
                245                 250                 255     


Glu Gly Asp Ser Cys Gly Gly Thr Tyr Ser Thr Asp Arg Tyr Ala Gly 
            260                 265                 270         


Ile Cys Asp Pro Asp Gly Cys Asp Phe Asn Ser Tyr Arg Gln Gly Asn 
        275                 280                 285             


Lys Thr Phe Tyr Gly Lys Gly Met Thr Val Asp Thr Thr Lys Lys Ile 
    290                 295                 300                 


Thr Val Val Thr Gln Phe Leu Lys Asn Ser Ala Gly Glu Leu Ser Glu 
305                 310                 315                 320 


Ile Lys Arg Phe Tyr Val Gln Asn Gly Lys Val Ile Pro Asn Ser Glu 
                325                 330                 335     


Ser Thr Ile Pro Gly Val Glu Gly Asn Ser Ile Thr Gln Asp Trp Cys 
            340                 345                 350         


Asp Arg Gln Lys Ala Ala Phe Gly Asp Val Thr Asp Phe Gln Asp Lys 
        355                 360                 365             


Gly Gly Met Val Gln Met Gly Lys Ala Leu Ala Gly Pro Met Val Leu 
    370                 375                 380                 


Val Met Ser Ile Trp Asp Asp His Ala Val Asn Met Leu Trp Leu Asp 
385                 390                 395                 400 


Ser Thr Trp Pro Ile Asp Gly Ala Gly Lys Pro Gly Ala Glu Arg Gly 
                405                 410                 415     


Ala Cys Pro Thr Thr Ser Gly Val Pro Ala Glu Val Glu Ala Glu Ala 
            420                 425                 430         


Pro Asn Ser Asn Val Ile Phe Ser Asn Ile Arg Phe Gly Pro Ile Gly 
        435                 440                 445             


Ser Thr Val Ser Gly Leu Pro Asp Gly Gly Ser Gly Asn Pro Asn Pro 
    450                 455                 460                 


Pro Val Ser Ser Ser Thr Pro Val Pro Ser Ser Ser Thr Thr Ser Ser 
465                 470                 475                 480 


Gly Ser Ser Gly Pro Thr Gly Gly Thr Gly Val Ala Lys His Tyr Glu 
                485                 490                 495     


Gln Cys Gly Gly Ile Gly Phe Thr Gly Pro Thr Gln Cys Glu Ser Pro 
            500                 505                 510         


Tyr Thr Cys Thr Lys Leu Asn Asp Trp Tyr Ser Gln Cys Leu 
        515                 520                 525     


<210>  9
<211>  509
<212>  PRT
<213>  Secuencia Artificial

<220>
<223>  Protena madura Cbh1N209E

<400>  9

Gln Asn Ala Cys Thr Leu Thr Ala Glu Asn His Pro Ser Leu Thr Trp 
1               5                   10                  15      


Ser Lys Cys Thr Ser Gly Gly Ser Cys Thr Ser Val Gln Gly Ser Ile 
            20                  25                  30          


Thr Ile Asp Ala Asn Trp Arg Trp Thr His Arg Thr Asp Ser Ala Thr 
        35                  40                  45              


Asn Cys Tyr Glu Gly Asn Lys Trp Asp Thr Ser Tyr Cys Ser Asp Gly 
    50                  55                  60                  


Pro Ser Cys Ala Ser Lys Cys Cys Ile Asp Gly Ala Asp Tyr Ser Ser 
65                  70                  75                  80  


Thr Tyr Gly Ile Thr Thr Ser Gly Asn Ser Leu Asn Leu Lys Phe Val 
                85                  90                  95      


Thr Lys Gly Gln Tyr Ser Thr Asn Ile Gly Ser Arg Thr Tyr Leu Met 
            100                 105                 110         


Glu Ser Asp Thr Lys Tyr Gln Met Phe Gln Leu Leu Gly Asn Glu Phe 
        115                 120                 125             


Thr Phe Asp Val Asp Val Ser Asn Leu Gly Cys Gly Leu Asn Gly Ala 
    130                 135                 140                 


Leu Tyr Phe Val Ser Met Asp Ala Asp Gly Gly Met Ser Lys Tyr Ser 
145                 150                 155                 160 


Gly Asn Lys Ala Gly Ala Lys Tyr Gly Thr Gly Tyr Cys Asp Ser Gln 
                165                 170                 175     


Cys Pro Arg Asp Leu Lys Phe Ile Asn Gly Glu Ala Asn Val Glu Glu 
            180                 185                 190         


Trp Gln Ser Ser Thr Asn Asp Ala Asn Ala Gly Thr Gly Lys Tyr Gly 
        195                 200                 205             


Ser Cys Cys Ser Glu Met Asp Val Trp Glu Ala Asn Asn Met Ala Ala 
    210                 215                 220                 


Ala Phe Thr Pro His Pro Cys Thr Val Ile Gly Gln Ser Arg Cys Glu 
225                 230                 235                 240 


Gly Asp Ser Cys Gly Gly Thr Tyr Ser Thr Asp Arg Tyr Ala Gly Ile 
                245                 250                 255     


Cys Asp Pro Asp Gly Cys Asp Phe Asn Ser Tyr Arg Gln Gly Asn Lys 
            260                 265                 270         


Thr Phe Tyr Gly Lys Gly Met Thr Val Asp Thr Thr Lys Lys Ile Thr 
        275                 280                 285             


Val Val Thr Gln Phe Leu Lys Asn Ser Ala Gly Glu Leu Ser Glu Ile 
    290                 295                 300                 


Lys Arg Phe Tyr Val Gln Asn Gly Lys Val Ile Pro Asn Ser Glu Ser 
305                 310                 315                 320 


Thr Ile Pro Gly Val Glu Gly Asn Ser Ile Thr Gln Asp Trp Cys Asp 
                325                 330                 335     


Arg Gln Lys Ala Ala Phe Gly Asp Val Thr Asp Phe Gln Asp Lys Gly 
            340                 345                 350         


Gly Met Val Gln Met Gly Lys Ala Leu Ala Gly Pro Met Val Leu Val 
        355                 360                 365             


Met Ser Ile Trp Asp Asp His Ala Val Asn Met Leu Trp Leu Asp Ser 
    370                 375                 380                 


Thr Trp Pro Ile Asp Gly Ala Gly Lys Pro Gly Ala Glu Arg Gly Ala 
385                 390                 395                 400 


Cys Pro Thr Thr Ser Gly Val Pro Ala Glu Val Glu Ala Glu Ala Pro 
                405                 410                 415     


Asn Ser Asn Val Ile Phe Ser Asn Ile Arg Phe Gly Pro Ile Gly Ser 
            420                 425                 430         


Thr Val Ser Gly Leu Pro Asp Gly Gly Ser Gly Asn Pro Asn Pro Pro 
        435                 440                 445             


Val Ser Ser Ser Thr Pro Val Pro Ser Ser Ser Thr Thr Ser Ser Gly 
    450                 455                 460                 


Ser Ser Gly Pro Thr Gly Gly Thr Gly Val Ala Lys His Tyr Glu Gln 
465                 470                 475                 480 


Cys Gly Gly Ile Gly Phe Thr Gly Pro Thr Gln Cys Glu Ser Pro Tyr 
                485                 490                 495     


Thr Cys Thr Lys Leu Asn Asp Trp Tyr Ser Gln Cys Leu 
            500                 505                 


<210>  10
<211>  52
<212>  DNA
<213>  Secuencia Artificial

<220>
<223>  Oligonucletido 1

<400>  10
ccgcggtggc ggccgctcta gacgctgcac tgtggcacga ctaccagtga tc               52


<210>  11
<211>  52
<212>  DNA
<213>  Secuencia Artificial

<220>
<223>  Oligonucletido 2

<400>  11
gctgcagccc gggggatccc caggctaatt gtcgcgtcgc ttcggacgga ca               52


<210>  12
<211>  52
<212>  DNA
<213>  Secuencia Artificial

<220>
<223>  Oligonucletido 3

<400>  12
catggtcata gaattcgata tcaacctctc tgaaggaggt tctgagacac gc               52


<210>  13
<211>  60
<212>  DNA
<213>  Secuencia Artificial

<220>
<223>  Oligonucletido 4

<400>  13
tgggtaccgg gccccccctc gagctagaag aagggcgtaa ataagaagct ataatagctt       60


<210>  14
<211>  22
<212>  DNA
<213>  Secuencia Artificial

<220>
<223>  Oligonucletido 5

<400>  14
aacaagtggg atacttcgta ct                                                22


<210>  15
<211>  21
<212>  DNA
<213>  Secuencia Artificial

<220>
<223>  Oligonucletido 6

<400>  15
atccatggac acgaagtaga g                                                 21


<210>  16
<211>  33
<212>  DNA
<213>  Secuencia Artificial

<220>
<223>  Oligonucletido 7

<400>  16
ccgacatatg aagcagtacc tccagtacct cgc                                    33


<210>  17
<211>  35
<212>  DNA
<213>  Secuencia Artificial

<220>
<223>  Oligonucletido 8

<400>  17
gctgaattct tagacgttga cagtcgagcc gatgg                                  35


<210>  18
<211>  26
<212>  DNA
<213>  Secuencia Artificial

<220>
<223>  Oligonucletido 9

<400>  18
gtgctgatcc tcttccgtcc catatg                                            26


<210>  19
<211>  24
<212>  DNA
<213>  Secuencia Artificial

<220>
<223>  Oligonucletido 10

<400>  19
ctcgaggtcg acggtatcga taag                                              24


