               SEQUENCE LISTING

<110> DSM IP Assets B.V.

<120> PHOSPHOLIPASE C

<130> 31117-WO-PCT

<160> 4

<170> BiSSAP 1.2

<210> 1
<211> 1929
<212> DNA
<213> Artificial sequence

<220> 
<221> source
<222> 1..1929
<223> /organism="Artificial sequence"
      /note="CpO sequence phospholipase C of Hypocrea virens L061"
      /mol_type="unassigned DNA"

<400> 1
atgcgccccg gctctgctct ctccctcctt gctcttggat ccgttgctca ggctgccatc      60

tccttggaag acagctctct ttctcctcgt gagattgaga acctcgagcg ttccatcgag     120

gctcgctctc ttgttgacga catctggaac gatatcaaga acgccgccac ctgcactgcc     180

tgccagggta tcctcgtcct cctcaagggt gttgccatct tcggtgacga tgcgtttgtc     240

tccattgcca ctggtctttg caagctggcc aaggttgagg atgacgatgt ctgcgagggt     300

actgttgctc ttgaggctcc catcattgcc gatgccatcc gcaacatgga ccttggatcg     360

gatacttcca agctcttctg cggctctttc ctgggcctct gccccgagcc ttctgttcct     420

cagtggaaga tccccttccc cagcagcaag ccctccaccg gtcgtcccgc tcctagcggc     480

aagactcctc tcaaggtcgt ccagtactcc gacatccaca tcgaccctct gtacgtgtct     540

ggctccacca ccaactgcac caagcccgtc tgctgccgtc cctacactgc tgccgatgag     600

cctggtcaca gcacctcgcc tgctggtccc aacggtgacc acaagtgcga cacccccgtc     660

ggtctggaaa tatcaatgta ccaggccatc aagaacattg tccccgatgc tgccttgact     720

ctcttcaccg gcgacattgt cgaccacgct atctggaaca cctccaagcc ctacaaccag     780

aagcaaatct ccgatgccta cacctacatg agccagtacc tgggtctggt ctacggcact     840

gctggcaacc acgaagcgga ccctgccaac gccttccctc cccagtccat ctccaacagc     900

agccagtggg tgtacgatgc cctttctgct cagtggactc gctgggttgg tgcttctgct     960

gaggctcaga tcgagaacat cggtgcctac tcgaccaagt accccaacgg caacctgcgt    1020

atcatctccc tgaacaccaa cttctactac cgcatgaact tctggctata ccaggaagac    1080

attgagcagg accccgatgg ccagatcaag tggttggtgt ctgagctgga tgctgctgag    1140

aaggccggtg agcgtgtcta catcattggc cacatgccca tcggtgaatc ggatgctttc    1200

cacgctggaa gcaactacat cgaccaggtt gtcaaccgct actcttccac cattgctgcc    1260

atgttcttcg gtcacaccca cgttgaccac ttcgaggtca gctactccga ctactccaag    1320

caggatgcct cccacgccgt catggccagc tacatctgcc cctccctgac ccccacctcc    1380

ggtatgccct ccttccgtgt ctacgatgtt gaccccgtga ccttcgccgt ccttgacacc    1440

accacctaca ttgccgacat gaccaacgcc aacttccaga ccactggtcc tgtctggacc    1500

aagctctact ctgccaagga agtctacggc tccaagctca accctcccgt gaccgacccc    1560

tccgctgagc tcacccccgc cttctggcac aacgtcaccg ctctcttcga gtccaactcc    1620

gccatgttca accagtacat ctccctcaag tcccgcggct ggaacgtcgc ctcctgcact    1680

ggtgactgcc agaagcagga gatctgccag ctccgtgctg gccgctccga gagcaactgc    1740

gttgttccca gccctggtct gcacatctcc aagcgctccg acgagcgtca cggacactct    1800

caccaccgtg ctcacgacca ccaggagtgc ggtatgagcg ctggtatgaa gaccattggc    1860

tccctggcca tgcgcaagga cctcctgaac gagctccagg tccgtgtcaa cgagctgcgc    1920

gccaaggcc                                                            1929


<210> 2
<211> 643
<212> PRT
<213> Hypocrea virens


<400> 2
Met Arg Pro Gly Ser Ala Leu Ser Leu Leu Ala Leu Gly Ser Val Ala 
1               5                   10                  15      
Gln Ala Ala Ile Ser Leu Glu Asp Ser Ser Leu Ser Pro Arg Glu Ile 
            20                  25                  30          
Glu Asn Leu Glu Arg Ser Ile Glu Ala Arg Ser Leu Val Asp Asp Ile 
        35                  40                  45              
Trp Asn Asp Ile Lys Asn Ala Ala Thr Cys Thr Ala Cys Gln Gly Ile 
    50                  55                  60                  
Leu Val Leu Leu Lys Gly Val Ala Ile Phe Gly Asp Asp Ala Phe Val 
65                  70                  75                  80  
Ser Ile Ala Thr Gly Leu Cys Lys Leu Ala Lys Val Glu Asp Asp Asp 
                85                  90                  95      
Val Cys Glu Gly Thr Val Ala Leu Glu Ala Pro Ile Ile Ala Asp Ala 
            100                 105                 110         
Ile Arg Asn Met Asp Leu Gly Ser Asp Thr Ser Lys Leu Phe Cys Gly 
        115                 120                 125             
Ser Phe Leu Gly Leu Cys Pro Glu Pro Ser Val Pro Gln Trp Lys Ile 
    130                 135                 140                 
Pro Phe Pro Ser Ser Lys Pro Ser Thr Gly Arg Pro Ala Pro Ser Gly 
145                 150                 155                 160 
Lys Thr Pro Leu Lys Val Val Gln Tyr Ser Asp Ile His Ile Asp Pro 
                165                 170                 175     
Leu Tyr Val Ser Gly Ser Thr Thr Asn Cys Thr Lys Pro Val Cys Cys 
            180                 185                 190         
Arg Pro Tyr Thr Ala Ala Asp Glu Pro Gly His Ser Thr Ser Pro Ala 
        195                 200                 205             
Gly Pro Asn Gly Asp His Lys Cys Asp Thr Pro Val Gly Leu Glu Ile 
    210                 215                 220                 
Ser Met Tyr Gln Ala Ile Lys Asn Ile Val Pro Asp Ala Ala Leu Thr 
225                 230                 235                 240 
Leu Phe Thr Gly Asp Ile Val Asp His Ala Ile Trp Asn Thr Ser Lys 
                245                 250                 255     
Pro Tyr Asn Gln Lys Gln Ile Ser Asp Ala Tyr Thr Tyr Met Ser Gln 
            260                 265                 270         
Tyr Leu Gly Leu Val Tyr Gly Thr Ala Gly Asn His Glu Ala Asp Pro 
        275                 280                 285             
Ala Asn Ala Phe Pro Pro Gln Ser Ile Ser Asn Ser Ser Gln Trp Val 
    290                 295                 300                 
Tyr Asp Ala Leu Ser Ala Gln Trp Thr Arg Trp Val Gly Ala Ser Ala 
305                 310                 315                 320 
Glu Ala Gln Ile Glu Asn Ile Gly Ala Tyr Ser Thr Lys Tyr Pro Asn 
                325                 330                 335     
Gly Asn Leu Arg Ile Ile Ser Leu Asn Thr Asn Phe Tyr Tyr Arg Met 
            340                 345                 350         
Asn Phe Trp Leu Tyr Gln Glu Asp Ile Glu Gln Asp Pro Asp Gly Gln 
        355                 360                 365             
Ile Lys Trp Leu Val Ser Glu Leu Asp Ala Ala Glu Lys Ala Gly Glu 
    370                 375                 380                 
Arg Val Tyr Ile Ile Gly His Met Pro Ile Gly Glu Ser Asp Ala Phe 
385                 390                 395                 400 
His Ala Gly Ser Asn Tyr Ile Asp Gln Val Val Asn Arg Tyr Ser Ser 
                405                 410                 415     
Thr Ile Ala Ala Met Phe Phe Gly His Thr His Val Asp His Phe Glu 
            420                 425                 430         
Val Ser Tyr Ser Asp Tyr Ser Lys Gln Asp Ala Ser His Ala Val Met 
        435                 440                 445             
Ala Ser Tyr Ile Cys Pro Ser Leu Thr Pro Thr Ser Gly Met Pro Ser 
    450                 455                 460                 
Phe Arg Val Tyr Asp Val Asp Pro Val Thr Phe Ala Val Leu Asp Thr 
465                 470                 475                 480 
Thr Thr Tyr Ile Ala Asp Met Thr Asn Ala Asn Phe Gln Thr Thr Gly 
                485                 490                 495     
Pro Val Trp Thr Lys Leu Tyr Ser Ala Lys Glu Val Tyr Gly Ser Lys 
            500                 505                 510         
Leu Asn Pro Pro Val Thr Asp Pro Ser Ala Glu Leu Thr Pro Ala Phe 
        515                 520                 525             
Trp His Asn Val Thr Ala Leu Phe Glu Ser Asn Ser Ala Met Phe Asn 
    530                 535                 540                 
Gln Tyr Ile Ser Leu Lys Ser Arg Gly Trp Asn Val Ala Ser Cys Thr 
545                 550                 555                 560 
Gly Asp Cys Gln Lys Gln Glu Ile Cys Gln Leu Arg Ala Gly Arg Ser 
                565                 570                 575     
Glu Ser Asn Cys Val Val Pro Ser Pro Gly Leu His Ile Ser Lys Arg 
            580                 585                 590         
Ser Asp Glu Arg His Gly His Ser His His Arg Ala His Asp His Gln 
        595                 600                 605             
Glu Cys Gly Met Ser Ala Gly Met Lys Thr Ile Gly Ser Leu Ala Met 
    610                 615                 620                 
Arg Lys Asp Leu Leu Asn Glu Leu Gln Val Arg Val Asn Glu Leu Arg 
625                 630                 635                 640 
Ala Lys Ala 
            

<210> 3
<211> 13
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..13
<223> /organism="Artificial Sequence"
      /note="translational initiation sequence of the glucoamylase glaA
      promoter"
      /mol_type="unassigned DNA"

<400> 3
caccgtcaaa atg                                                       13


<210> 4
<211> 2145
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..2145
<223> /organism="Artificial Sequence"
      /note="L061-insert Artificial sequence"
      /mol_type="unassigned DNA"

<400> 4
gaattcaagc tagatgctaa gcgatattgc atggcaatat gtgttgatgc atgtgcttct      60

tccttcagct tcccctcgtg cagatgaggt ttggctataa attgaagtgg ttggtcgggg     120

ttccgtgagg ggctgaagtg cttcctccct tttagacgca actgagagcc tgagcttcat     180

ccccagcatc attacaccgt caaaatgcgc cccggctctg ctctctccct ccttgctctt     240

ggatccgttg ctcaggctgc catctccttg gaagacagct ctctttctcc tcgtgagatt     300

gagaacctcg agcgttccat cgaggctcgc tctcttgttg acgacatctg gaacgatatc     360

aagaacgccg ccacctgcac tgcctgccag ggtatcctcg tcctcctcaa gggtgttgcc     420

atcttcggtg acgatgcgtt tgtctccatt gccactggtc tttgcaagct ggccaaggtt     480

gaggatgacg atgtctgcga gggtactgtt gctcttgagg ctcccatcat tgccgatgcc     540

atccgcaaca tggaccttgg atcggatact tccaagctct tctgcggctc tttcctgggc     600

ctctgccccg agccttctgt tcctcagtgg aagatcccct tccccagcag caagccctcc     660

accggtcgtc ccgctcctag cggcaagact cctctcaagg tcgtccagta ctccgacatc     720

cacatcgacc ctctgtacgt gtctggctcc accaccaact gcaccaagcc cgtctgctgc     780

cgtccctaca ctgctgccga tgagcctggt cacagcacct cgcctgctgg tcccaacggt     840

gaccacaagt gcgacacccc cgtcggtctg gaaatatcaa tgtaccaggc catcaagaac     900

attgtccccg atgctgcctt gactctcttc accggcgaca ttgtcgacca cgctatctgg     960

aacacctcca agccctacaa ccagaagcaa atctccgatg cctacaccta catgagccag    1020

tacctgggtc tggtctacgg cactgctggc aaccacgaag cggaccctgc caacgccttc    1080

cctccccagt ccatctccaa cagcagccag tgggtgtacg atgccctttc tgctcagtgg    1140

actcgctggg ttggtgcttc tgctgaggct cagatcgaga acatcggtgc ctactcgacc    1200

aagtacccca acggcaacct gcgtatcatc tccctgaaca ccaacttcta ctaccgcatg    1260

aacttctggc tataccagga agacattgag caggaccccg atggccagat caagtggttg    1320

gtgtctgagc tggatgctgc tgagaaggcc ggtgagcgtg tctacatcat tggccacatg    1380

cccatcggtg aatcggatgc tttccacgct ggaagcaact acatcgacca ggttgtcaac    1440

cgctactctt ccaccattgc tgccatgttc ttcggtcaca cccacgttga ccacttcgag    1500

gtcagctact ccgactactc caagcaggat gcctcccacg ccgtcatggc cagctacatc    1560

tgcccctccc tgacccccac ctccggtatg ccctccttcc gtgtctacga tgttgacccc    1620

gtgaccttcg ccgtccttga caccaccacc tacattgccg acatgaccaa cgccaacttc    1680

cagaccactg gtcctgtctg gaccaagctc tactctgcca aggaagtcta cggctccaag    1740

ctcaaccctc ccgtgaccga cccctccgct gagctcaccc ccgccttctg gcacaacgtc    1800

accgctctct tcgagtccaa ctccgccatg ttcaaccagt acatctccct caagtcccgc    1860

ggctggaacg tcgcctcctg cactggtgac tgccagaagc aggagatctg ccagctccgt    1920

gctggccgct ccgagagcaa ctgcgttgtt cccagccctg gtctgcacat ctccaagcgc    1980

tccgacgagc gtcacggaca ctctcaccac cgtgctcacg accaccagga gtgcggtatg    2040

agcgctggta tgaagaccat tggctccctg gccatgcgca aggacctcct gaacgagctc    2100

caggtccgtg tcaacgagct gcgcgccaag gcctaaatta attaa                    2145


