                         SEQUENCE LISTING

<110>  Mirza, et al.
 
<120>  Treatment of cancer using inhibitors of TGF-Beta and PD-1

<130>  27129/49343

<150>  US 62/191,797
<151>  2015-07-13

<150>  US 62/143,016
<151>  2015-04-03

<160>  30    

<170>  PatentIn version 3.5

<210>  1
<211>  366
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic nucleotide


<220>
<221>  misc_feature
<223>  Clone XPA.42.068 heavy chain

<400>  1
caggttcagc tggtgcagtc tggggctgag gtgaagaagc ctggggcctc agtgaaggtc       60

tcctgcaagg cttctggata caccttcacc ggctactata tgcactgggt gcgacaggcc      120

cctggacaag ggcttgagtg gatgggatgg atcaacccta acactggtgg cacaaactat      180

gcacagaagt ttcagggcag ggtcaccatg accagggaca cgtccatcag cacagcctac      240

atggagctga gcaggctgag atctgacgac acggccgttt attactgtgc gagatcattc      300

ctgtggctgg ttccctctga tgcttttgat atctggggcc aagggacaat ggtcaccgtc      360

tcttca                                                                 366


<210>  2
<211>  122
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<223>  Clone XPA.42.068 heavy chain

<220>
<221>  MISC_FEATURE
<222>  (26)..(33)
<223>  H-CDR1

<220>
<221>  MISC_FEATURE
<222>  (51)..(58)
<223>  H-CDR2

<220>
<221>  MISC_FEATURE
<222>  (97)..(111)
<223>  H-CDR3

<400>  2

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Gly Tyr 
            20                  25                  30          


Tyr Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Trp Ile Asn Pro Asn Thr Gly Gly Thr Asn Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Met Thr Arg Asp Thr Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Phe Leu Trp Leu Val Pro Ser Asp Ala Phe Asp Ile Trp 
            100                 105                 110         


Gly Gln Gly Thr Met Val Thr Val Ser Ser 
        115                 120         


<210>  3
<211>  327
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic nucleotide


<220>
<221>  misc_feature
<223>  Clone XPA.42.068 light chain

<400>  3
tcttctgagc tgactcagcc accctcagtg tccgtggccc caggagagaa ggccaggatt       60

acctgtgggg ggaataacat tggacgtaaa agtgtacatt ggtaccagca gaggccaggc      120

caggcccctg ttgtggtcct ctactatgat agagtcagac cctcagggat ccctgagcga      180

ttttctggct ccaactctgg gaacacggcc accctgacca tcaccagggt cgaagccggg      240

gatgaggccg actatttttg tcaggtgtgg gataacacta gtgagcatgt ggtcttcggc      300

ggaggcaccc agctgaccgt cctaggc                                          327


<210>  4
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<223>  Clone XPA.42.068 light chain

<220>
<221>  MISC_FEATURE
<222>  (26)..(31)
<223>  L-CDR1

<220>
<221>  MISC_FEATURE
<222>  (49)..(51)
<223>  L-CDR2

<220>
<221>  MISC_FEATURE
<222>  (88)..(97)
<223>  L-CDR3

<400>  4

Ser Ser Glu Leu Thr Gln Pro Pro Ser Val Ser Val Ala Pro Gly Glu 
1               5                   10                  15      


Lys Ala Arg Ile Thr Cys Gly Gly Asn Asn Ile Gly Arg Lys Ser Val 
            20                  25                  30          


His Trp Tyr Gln Gln Arg Pro Gly Gln Ala Pro Val Val Val Leu Tyr 
        35                  40                  45              


Tyr Asp Arg Val Arg Pro Ser Gly Ile Pro Glu Arg Phe Ser Gly Ser 
    50                  55                  60                  


Asn Ser Gly Asn Thr Ala Thr Leu Thr Ile Thr Arg Val Glu Ala Gly 
65                  70                  75                  80  


Asp Glu Ala Asp Tyr Phe Cys Gln Val Trp Asp Asn Thr Ser Glu His 
                85                  90                  95      


Val Val Phe Gly Gly Gly Thr Gln Leu Thr Val Leu Gly 
            100                 105                 


<210>  5
<211>  363
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic nucleotide


<220>
<221>  misc_feature
<223>  Clone XPA.42.089 heavy chain

<400>  5
caggtgcagc tggtacagtc tggggctgag gtgaagaagc ctgggtcctc ggtgaaggtc       60

tcctgcaagg cttctggagg caccttcagc agctatgcta tcagctgggt gcgacaggcc      120

cctggacaag ggcttgagtg gatgggaggg atcatcccta tctttggtac agcaaactac      180

gcacagaagt tccagggcag agtcacgatt accgcggacg aatccacgag cacagcctac      240

atggagctga gcagcctgag atctgaggac acggccgtgt attactgtgc gaggggacta      300

tgggaggttc gggcccttcc gtcggtctac tggggccagg gaaccctggt caccgtctcc      360

tca                                                                    363


<210>  6
<211>  121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<223>  Clone XPA.42.089 heavy chain

<220>
<221>  MISC_FEATURE
<222>  (26)..(33)
<223>  H-CDR1

<220>
<221>  MISC_FEATURE
<222>  (51)..(58)
<223>  H-CDR2

<220>
<221>  MISC_FEATURE
<222>  (97)..(110)
<223>  H-CDR3

<400>  6

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Gly Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Ile Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Gly Ile Ile Pro Ile Phe Gly Thr Ala Asn Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Ala Asp Glu Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Leu Trp Glu Val Arg Ala Leu Pro Ser Val Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120     


<210>  7
<211>  327
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic nucleotide


<220>
<221>  misc_feature
<223>  Clone XPA.42.089 light chain

<400>  7
tcctatgagc tgacacagcc accctcagtg tccgtggccc cgggacagac ggccagaatt       60

acctgtgggg caaatgacat tggaagtaaa agtgtccact ggtaccagca gaaggcaggc      120

caggcccctg tactggtcgt ctctgaagat atcatccggc cctcagggat ccctgagcga      180

atctctggct ccaactctgg gaacacggcc accctgacca tcagcagggt cgaagccggg      240

gatgaggccg actattactg tcaagtttgg gatagggata gtgatcaata tgtctttgga      300

actgggacca aggtcaccgt cctaggc                                          327


<210>  8
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<223>  Clone XPA.42.089 light chain

<220>
<221>  MISC_FEATURE
<222>  (26)..(31)
<223>  L-CDR1

<220>
<221>  MISC_FEATURE
<222>  (49)..(51)
<223>  L-CDR2

<220>
<221>  MISC_FEATURE
<222>  (88)..(97)
<223>  L-CDR3

<400>  8

Ser Tyr Glu Leu Thr Gln Pro Pro Ser Val Ser Val Ala Pro Gly Gln 
1               5                   10                  15      


Thr Ala Arg Ile Thr Cys Gly Ala Asn Asp Ile Gly Ser Lys Ser Val 
            20                  25                  30          


His Trp Tyr Gln Gln Lys Ala Gly Gln Ala Pro Val Leu Val Val Ser 
        35                  40                  45              


Glu Asp Ile Ile Arg Pro Ser Gly Ile Pro Glu Arg Ile Ser Gly Ser 
    50                  55                  60                  


Asn Ser Gly Asn Thr Ala Thr Leu Thr Ile Ser Arg Val Glu Ala Gly 
65                  70                  75                  80  


Asp Glu Ala Asp Tyr Tyr Cys Gln Val Trp Asp Arg Asp Ser Asp Gln 
                85                  90                  95      


Tyr Val Phe Gly Thr Gly Thr Lys Val Thr Val Leu Gly 
            100                 105                 


<210>  9
<211>  366
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic nucleotide


<220>
<221>  misc_feature
<223>  Clone XPA.42.681 heavy chain

<400>  9
caggttcagc tggtgcagtc tggggctgag gtgaagaagc ctggggcctc agtgaaggtc       60

tcctgcaagg cttctggata caccttcacc ggctactata tgcactgggt gcgacaggcc      120

cctggacaag ggcttgagtg gatgggatgg atcaacccta acactggtgg cacaaactat      180

gcacagaagt ttcagggcag ggtcaccatg accagggaca cgtccatcag cacagcctac      240

atggagctga gcaggctgag atctgacgac acggccgttt attactgtgc gagatcattc      300

ctgtggctgg ttccctctga tgcttttgat atctggggcc aagggacaat ggtcaccgtc      360

tcttca                                                                 366


<210>  10
<211>  122
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<223>  Clone XPA.42.681 heavy chain

<220>
<221>  MISC_FEATURE
<222>  (26)..(33)
<223>  H-CDR1

<220>
<221>  MISC_FEATURE
<222>  (51)..(58)
<223>  H-CDR2

<220>
<221>  MISC_FEATURE
<222>  (97)..(111)
<223>  H-CDR3

<400>  10

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Gly Tyr 
            20                  25                  30          


Tyr Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Trp Ile Asn Pro Asn Thr Gly Gly Thr Asn Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Met Thr Arg Asp Thr Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Phe Leu Trp Leu Val Pro Ser Asp Ala Phe Asp Ile Trp 
            100                 105                 110         


Gly Gln Gly Thr Met Val Thr Val Ser Ser 
        115                 120         


<210>  11
<211>  327
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic nucleotide


<220>
<221>  misc_feature
<223>  Clone XPA.42.681 light chain

<400>  11
tcctatgtgc tgactcagcc accctcagtg tccgtggccc caggaaagac ggccaggatt       60

acctgtgggg gaaacaacat tggatttaga agtgtgcact ggtaccaaca gaagtcaggc      120

caggcccctg tcctggtcat ctattttgat cgcgcccggc cctcagggat ccctgagcga      180

ttctctgcct ccaactctga gaacacggcc accctgacca tcaggagggt cgaagccggg      240

gatgaggccg actattactg tcaggtgtgg gatagtgaca gtgatgatct agtcttcggc      300

ggaggcaccc agctgaccgt cctaggt                                          327


<210>  12
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<223>  Clone XPA.42.681 light chain

<220>
<221>  MISC_FEATURE
<222>  (26)..(31)
<223>  L-CDR1

<220>
<221>  MISC_FEATURE
<222>  (49)..(51)
<223>  L-CDR2

<220>
<221>  MISC_FEATURE
<222>  (88)..(98)
<223>  L-CDR3

<400>  12

Ser Tyr Val Leu Thr Gln Pro Pro Ser Val Ser Val Ala Pro Gly Lys 
1               5                   10                  15      


Thr Ala Arg Ile Thr Cys Gly Gly Asn Asn Ile Gly Phe Arg Ser Val 
            20                  25                  30          


His Trp Tyr Gln Gln Lys Ser Gly Gln Ala Pro Val Leu Val Ile Tyr 
        35                  40                  45              


Phe Asp Arg Ala Arg Pro Ser Gly Ile Pro Glu Arg Phe Ser Ala Ser 
    50                  55                  60                  


Asn Ser Glu Asn Thr Ala Thr Leu Thr Ile Arg Arg Val Glu Ala Gly 
65                  70                  75                  80  


Asp Glu Ala Asp Tyr Tyr Cys Gln Val Trp Asp Ser Asp Ser Asp Asp 
                85                  90                  95      


Leu Val Phe Gly Gly Gly Thr Gln Leu Thr Val Leu Gly 
            100                 105                 


<210>  13
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<223>  Clone XPA.42.068 - H-CDR1

<400>  13

Gly Tyr Thr Phe Thr Gly Tyr Tyr 
1               5               


<210>  14
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<223>  Clone XPA.42.068 - H-CDR2

<400>  14

Ile Asn Pro Asn Thr Gly Gly Thr 
1               5               


<210>  15
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<223>  Clone XPA.42.068 - H-CDR3

<400>  15

Ala Arg Ser Phe Leu Trp Leu Val Pro Ser Asp Ala Phe Asp Ile 
1               5                   10                  15  


<210>  16
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<223>  Clone XPA.42.068 - L-CDR1

<400>  16

Asn Ile Gly Arg Lys Ser 
1               5       


<210>  17
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<223>  Clone XPA.42.068 - L-CDR2

<400>  17

Tyr Asp Arg 
1           


<210>  18
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<223>  Clone XPA.42.068 - L-CDR3

<400>  18

Gln Val Trp Asp Asn Thr Ser Glu His Val 
1               5                   10  


<210>  19
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<223>  Clone XPA.42.089 - H-CDR1

<400>  19

Gly Gly Thr Phe Ser Ser Tyr Ala 
1               5               


<210>  20
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<223>  Clone XPA.42.089 - H-CDR2

<400>  20

Ile Ile Pro Ile Phe Gly Thr Ala 
1               5               


<210>  21
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<223>  Clone XPA.42.089 - H-CDR3

<400>  21

Ala Arg Gly Leu Trp Glu Val Arg Ala Leu Pro Ser Val Tyr 
1               5                   10                  


<210>  22
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<223>  Clone XPA.42.089 - L-CDR1

<400>  22

Asp Ile Gly Ser Lys Ser 
1               5       


<210>  23
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<223>  Clone XPA.42.089 - L-CDR2

<400>  23

Glu Asp Ile 
1           


<210>  24
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<223>  Clone XPA.42.089 - L-CDR3

<400>  24

Gln Val Trp Asp Arg Asp Ser Asp Gln Tyr 
1               5                   10  


<210>  25
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<223>  Clone XPA.42-681 - H-CDR1

<400>  25

Gly Tyr Thr Phe Thr Gly Tyr Tyr 
1               5               


<210>  26
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<223>  Clone XPA.42-681 - H-CDR2

<400>  26

Ile Asn Pro Asn Thr Gly Gly Thr 
1               5               


<210>  27
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<223>  Clone XPA.42-681 - H-CDR3

<400>  27

Ala Arg Ser Phe Leu Trp Leu Val Pro Ser Asp Ala Phe Asp Ile 
1               5                   10                  15  


<210>  28
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<223>  Clone XPA.42-681 - L-CDR1

<400>  28

Asn Ile Gly Phe Arg Ser 
1               5       


<210>  29
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<223>  Clone XPA.42-681 - L-CDR2

<400>  29

Phe Asp Arg 
1           


<210>  30
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<223>  Clone XPA.42-681 - L-CDR3

<400>  30

Gln Val Trp Asp Ser Asp Ser Asp Asp Leu Val 
1               5                   10      


