
                                SEQUENCE LISTING

<110> Bielas, Jason H.
      Gregory Mark, T.

<120> COMPOSITIONS AND METHODS FOR TARGET
  NUCLEIC ACID MOLECULE ENRICHMENT
  

<130> 360056.432WO

<140> PCT                 
<141> 2016-03-31  

<150> US 62/140,930       
<151> 2015-03-31  

<160> 22

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 897
<212> DNA
<213> Artificial Sequence

<220> 
<223> Sequencing cassette

<220>
<221> misc_feature   
<222> (1)...(76)
<223> Adaptor sequence

<220>
<221> misc_feature   
<222> (77)...(90)
<223> 14 nucleotide bar code

<220>
<221> misc_feature   
<222> (77)...(90)
<223> n = A, T, C or G

<220>
<221> misc_feature   
<222> (91)...(105)
<223> XcmI restriction site

<220>
<221> misc_feature   
<222> (106)...(882)
<223> GFP insert

<400> 1
caagcagaag acggcatacg agatctagta cgcggtctgc cttgccagcc cgctcagaga      60
tgtgtataag agacagnnnn nnnnnnnnnn ccaatactcg agtggttgac agctagctca     120
gtcctaggta taatgctagc caatttcaca aggaggcagc taatggtgag caagggcgag     180
gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt gaacggccac     240
aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag     300
ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc     360
tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag     420
tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac     480
tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg     540
aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac     600
aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc     660
aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac     720
acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc     780
gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc     840
gccgccggga tcactcacgg catggacgag ctgtacaagt aaccatacca tggttgg        897

<210> 2
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 2
caagcagaag acggcatacg a                                                21

<210> 3
<211> 92
<212> DNA
<213> Artificial Sequence

<220> 
<223> Targeting primer

<400> 3
aatgatacgg cgaccaccga gatctacact agatcgcgcc tccctcgcgc catcagagat      60
gtgtataaga gacaggctca tggtgggggc ag                                    92

<210> 4
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 4
aatgatacgg cgaccaccga                                                  20

<210> 5
<211> 7
<212> DNA
<213> Artificial Sequence

<220> 
<223> 7 nucleotide barcode

<220>
<221> misc_feature   
<222> (1)...(7)
<223> n = A,T,C or G

<400> 5
nnnnnnn                                                                 7

<210> 6
<211> 3568
<212> DNA
<213> Artificial Sequence

<220> 
<223> Vector Sequence with GFP insert

<220>
<221> misc_feature   
<222> (402)...(477)
<223> Adaptor sequence

<220>
<221> misc_feature   
<222> (478)...(491)
<223> 14 nucleotide bar code

<220>
<221> misc_feature   
<222> (478)...(491)
<223> n = A,T,C or G

<220>
<221> misc_feature   
<222> (492)...(506)
<223> XcmI restriction site

<220>
<221> misc_feature   
<222> (507)...(1283)
<223> GFP insert

<220>
<221> misc_feature   
<222> (1284)...(1298)
<223> XcmI restriction site

<400> 6
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca      60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg     120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc     180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc     240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat     300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt     360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ccaagcagaa gacggcatac     420
gagatctagt acgcggtctg ccttgccagc ccgctcagag atgtgtataa gagacagnnn     480
nnnnnnnnnn nccaatactc gagtggttga cagctagctc agtcctaggt ataatgctag     540
ccaatttcac aaggaggcag ctaatggtga gcaagggcga ggagctgttc accggggtgg     600
tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg     660
agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca     720
agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca     780
gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct     840
acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg     900
tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg     960
aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata    1020
tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg    1080
aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc    1140
ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca    1200
acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg    1260
gcatggacga gctgtacaag taaccatacc atggttgggg atcctctaga gtcgacctgc    1320
aggcatgcaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg    1380
ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa    1440
tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac    1500
ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt    1560
gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga    1620
gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca    1680
ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg    1740
ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt    1800
cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc    1860
ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct    1920
tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc    1980
gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta    2040
tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca    2100
gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag    2160
tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag    2220
ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt    2280
agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa    2340
gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg    2400
attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga    2460
agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta    2520
atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc    2580
cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg    2640
ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga    2700
agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt    2760
tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt    2820
gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc    2880
caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc    2940
ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca    3000
gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag    3060
tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg    3120
tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa    3180
cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa    3240
cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga    3300
gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga    3360
atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg    3420
agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt    3480
ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa    3540
aataggcgta tcacgaggcc ctttcgtc                                       3568

<210> 7
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<223> Sense strand index sequence

<220>
<221> misc_feature   
<222> (16)...(18)
<223> non-complementary sequence

<400> 7
gcatagcgca tgcagcccca ctagcgactc gat                                   33

<210> 8
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> Antisense strand index sequence

<220>
<221> misc_feature   
<222> (16)...(18)
<223> non-complementary sequence

<400> 8
tcgagtcgct agtgcttttg catgcgctat gc                                    32

<210> 9
<211> 76
<212> DNA
<213> Artificial Sequence

<220> 
<223> Nextera Adaptor Sequence

<400> 9
caagcagaag acggcatacg agatctagta cgcggtctgc cttgccagcc cgctcagaga      60
tgtgtataag agacag                                                      76

<210> 10
<211> 75
<212> DNA
<213> Artificial Sequence

<220> 
<223> Nextera Adaptor Sequence

<400> 10
aatgatacgg cgaccaccga gatctacact agatcgcgcc tccctcgcgc catcagagat      60
gtgtataaga gacag                                                       75

<210> 11
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> Nextera Adaptor Sequence

<400> 11
caagcagaag acggcatacg agat                                             24

<210> 12
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<223> Nextera Adaptor Sequence

<400> 12
aatgatacgg cgaccaccga gatctacac                                        29

<210> 13
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> Ion Xpress Adaptor Sequence 1

<400> 13
ccatctcatc cctgcgtgtc tccgactcag                                       30

<210> 14
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> Ion Xpress Adaptor Sequence 2

<400> 14
cctctctatg ggcagtcggt gat                                              23

<210> 15
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Roche 454 Adaptor Sequence 1

<400> 15
cgtatcgcct ccctcgcgcc atcag                                            25

<210> 16
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Roche 454 Adaptor Sequence 2

<400> 16
ctatgcgcct tgccagcccg ctcag                                            25

<210> 17
<211> 14
<212> DNA
<213> Artificial Sequence

<220> 
<223> 14 nucleotide barcode

<220>
<221> misc_feature   
<222> (1)...(14)
<223> n = A,T,C or G

<400> 17
nnnnnnnnnn nnnn                                                        14

<210> 18
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> p53 exon 5-6 Forward primer

<400> 18
cacttgtgcc ctgactttca                                                  20

<210> 19
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> p53 exon 5-6 Reverse primer

<400> 19
cttaacccct cctcccagag                                                  20

<210> 20
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> Nested p53 exon 5 primer

<220>
<221> misc_feature   
<222> (1)...(33)
<223> Adaptor read primer

<220>
<221> misc_feature   
<222> (34)...(50)
<223> p53 exon 5 specific sequence

<400> 20
tcgtcggcag cgtcagatgt gtataagaga caggctcatg gtgggggcag                 50

<210> 21
<211> 51
<212> DNA
<213> Artificial Sequence

<220> 
<223> Nextera Index primer S505

<220>
<221> misc_feature   
<222> (1)...(29)
<223> flow cell adaptor sequence

<220>
<221> misc_feature   
<222> (30)...(37)
<223> index sequence

<220>
<221> misc_feature   
<222> (38)...(51)
<223> Adaptor read primer

<400> 21
aatgatacgg cgaccaccga gatctacacg taaggagtcg tcggcagcgt c               51

<210> 22
<211> 51
<212> DNA
<213> Artificial Sequence

<220> 
<223> Nextera Index primer S506

<220>
<221> misc_feature   
<222> (1)...(29)
<223> flow cell adaptor sequence

<220>
<221> misc_feature   
<222> (30)...(37)
<223> index sequence

<220>
<221> misc_feature   
<222> (38)...(51)
<223> Adaptor read primer

<400> 22
aatgatacgg cgaccaccga gatctacaca ctgcatatcg tcggcagcgt c               51
