                         SEQUENCE LISTING

<110>  THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA
       WEINER, David
       VILLARREAL, Daniel
 
<120>  ISG15 AND ITS USE AS AN ADJUVANT

<130>  UPVG0074 WO

<150>  62136325
<151>  2015-03-20

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  534
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mut ISG15 mouse DNA

<400>  1
atggactgga cctggattct gttcctcgtc gccgctgcta caagagtgca ttccgcctgg       60

gacctcaaag tgaagatgct cgggggtaac gacttcctgg tgtcagtcac taatagcatg      120

accgtgtccg agctgaagaa acagatcgca cagaaaattg gcgtcccagc ctttcagcag      180

aggctggccc accagacagc tgtgctccag gatggactga ctctcagctc cctgggcctc      240

ggaccctcta gtaccgtgat gctggtggtc cagaactgct ctgaacctct gagtatcctc      300

gtgaggaacg agagagggca ctctaatatc tacgaagtgt tcctgaccca gacagtcgac      360

acactcaaga aaaaggtgtc ccagcgggag caggtccatg aagaccagtt ctggctgagc      420

tttgagggac gccccatgga ggataaagaa ctgctcgggg aatatggtct gaagccccag      480

tgtaccgtca tcaaacatgc agcagcagca ggtggaggcg gagaccagtg tgcc            534


<210>  2
<211>  178
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mut ISG15 mouse protein

<400>  2

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Ala Trp Asp Leu Lys Val Lys Met Leu Gly Gly Asn Asp Phe 
            20                  25                  30          


Leu Val Ser Val Thr Asn Ser Met Thr Val Ser Glu Leu Lys Lys Gln 
        35                  40                  45              


Ile Ala Gln Lys Ile Gly Val Pro Ala Phe Gln Gln Arg Leu Ala His 
    50                  55                  60                  


Gln Thr Ala Val Leu Gln Asp Gly Leu Thr Leu Ser Ser Leu Gly Leu 
65                  70                  75                  80  


Gly Pro Ser Ser Thr Val Met Leu Val Val Gln Asn Cys Ser Glu Pro 
                85                  90                  95      


Leu Ser Ile Leu Val Arg Asn Glu Arg Gly His Ser Asn Ile Tyr Glu 
            100                 105                 110         


Val Phe Leu Thr Gln Thr Val Asp Thr Leu Lys Lys Lys Val Ser Gln 
        115                 120                 125             


Arg Glu Gln Val His Glu Asp Gln Phe Trp Leu Ser Phe Glu Gly Arg 
    130                 135                 140                 


Pro Met Glu Asp Lys Glu Leu Leu Gly Glu Tyr Gly Leu Lys Pro Gln 
145                 150                 155                 160 


Cys Thr Val Ile Lys His Ala Ala Ala Ala Gly Gly Gly Gly Asp Gln 
                165                 170                 175     


Cys Ala 
        


<210>  3
<211>  534
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  wt ISG15 mouse DNA

<400>  3
atggactgga catggattct gtttctggtg gcagccgcta ctcgggtgca tagtgcttgg       60

gacctcaaag tgaagatgct cgggggtaac gacttcctgg tgtcagtcac taatagcatg      120

accgtgtccg agctgaagaa acagatcgca cagaaaattg gagtcccagc atttcagcag      180

aggctggcac accagacagc tgtgctccag gatggactga ctctcagctc cctgggcctc      240

ggaccctcta gtaccgtgat gctggtggtc cagaactgct ctgaacctct gagtatcctc      300

gtgcggaacg agcgcgggca ctctaatatc tacgaagtgt tcctgaccca gacagtcgac      360

acactcaaga aaaaggtgtc ccagcgagag caggtccatg aagaccagtt ctggctgagc      420

tttgagggca gaccaatgga ggataaggaa ctgctcgggg aatatggtct gaaaccccag      480

tgcaccgtga tcaagcatct gaggctcaga ggaggaggag gcgaccagtg tgct            534


<210>  4
<211>  178
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  wt ISG15 mouse protein

<400>  4

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Ala Trp Asp Leu Lys Val Lys Met Leu Gly Gly Asn Asp Phe 
            20                  25                  30          


Leu Val Ser Val Thr Asn Ser Met Thr Val Ser Glu Leu Lys Lys Gln 
        35                  40                  45              


Ile Ala Gln Lys Ile Gly Val Pro Ala Phe Gln Gln Arg Leu Ala His 
    50                  55                  60                  


Gln Thr Ala Val Leu Gln Asp Gly Leu Thr Leu Ser Ser Leu Gly Leu 
65                  70                  75                  80  


Gly Pro Ser Ser Thr Val Met Leu Val Val Gln Asn Cys Ser Glu Pro 
                85                  90                  95      


Leu Ser Ile Leu Val Arg Asn Glu Arg Gly His Ser Asn Ile Tyr Glu 
            100                 105                 110         


Val Phe Leu Thr Gln Thr Val Asp Thr Leu Lys Lys Lys Val Ser Gln 
        115                 120                 125             


Arg Glu Gln Val His Glu Asp Gln Phe Trp Leu Ser Phe Glu Gly Arg 
    130                 135                 140                 


Pro Met Glu Asp Lys Glu Leu Leu Gly Glu Tyr Gly Leu Lys Pro Gln 
145                 150                 155                 160 


Cys Thr Val Ile Lys His Leu Arg Leu Arg Gly Gly Gly Gly Asp Gln 
                165                 170                 175     


Cys Ala 
        


<210>  5
<211>  546
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mut ISG15 human vers.1 DNA

<400>  5
atggactgga cttggattct gtttctggtc gccgctgcta ctcgcgtgca ttctggctgg       60

gacctgactg tgaagatgct ggctggaaac gagttccagg tgtccctgag ctcctctatg      120

agcgtctccg aactgaaggc tcagatcaca cagaaaattg gggtgcacgc ctttcagcag      180

aggctggctg tgcatccttc tggagtcgca ctgcaggacc gagtgccact ggcatctcag      240

ggactgggac caggaagtac tgtgctgctg gtggtcgaca agtgcgatga gccactgtca      300

atcctggtgc ggaacaacaa gggcagaagt tcaacctacg aagtgcgact gacccagaca      360

gtcgcccacc tgaagcagca ggtgagcgga ctggagggag tccaggacga tctgttctgg      420

ctgacctttg aggggaagcc cctggaagat cagctgcctc tgggagaata tggcctgaaa      480

cccctgtcca ctgtctttat gaatgccaga gccagaggag ccgccactga gccaggggga      540

aggagc                                                                 546


<210>  6
<211>  182
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mut ISG15 human vers.1 protein

<400>  6

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Gly Trp Asp Leu Thr Val Lys Met Leu Ala Gly Asn Glu Phe 
            20                  25                  30          


Gln Val Ser Leu Ser Ser Ser Met Ser Val Ser Glu Leu Lys Ala Gln 
        35                  40                  45              


Ile Thr Gln Lys Ile Gly Val His Ala Phe Gln Gln Arg Leu Ala Val 
    50                  55                  60                  


His Pro Ser Gly Val Ala Leu Gln Asp Arg Val Pro Leu Ala Ser Gln 
65                  70                  75                  80  


Gly Leu Gly Pro Gly Ser Thr Val Leu Leu Val Val Asp Lys Cys Asp 
                85                  90                  95      


Glu Pro Leu Ser Ile Leu Val Arg Asn Asn Lys Gly Arg Ser Ser Thr 
            100                 105                 110         


Tyr Glu Val Arg Leu Thr Gln Thr Val Ala His Leu Lys Gln Gln Val 
        115                 120                 125             


Ser Gly Leu Glu Gly Val Gln Asp Asp Leu Phe Trp Leu Thr Phe Glu 
    130                 135                 140                 


Gly Lys Pro Leu Glu Asp Gln Leu Pro Leu Gly Glu Tyr Gly Leu Lys 
145                 150                 155                 160 


Pro Leu Ser Thr Val Phe Met Asn Ala Arg Ala Arg Gly Ala Ala Thr 
                165                 170                 175     


Glu Pro Gly Gly Arg Ser 
            180         


<210>  7
<211>  546
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  wt ISG15 human DNA

<400>  7
atggattgga cttggattct gttcctggtc gctgccgcta ctcgcgtgca ctctggatgg       60

gacctgaccg tgaaaatgct ggctggaaac gagttccagg tgtccctgag ctcctctatg      120

agcgtctccg aactgaaggc tcagatcaca cagaaaattg gggtgcacgc ctttcagcag      180

aggctggctg tgcatccttc tggagtcgca ctgcaggacc gagtgccact ggcatctcag      240

ggactgggac caggaagtac tgtgctgctg gtggtcgaca agtgcgatga gccactgtca      300

atcctggtgc ggaacaacaa gggcagaagt tcaacctacg aagtgcgact gacccagaca      360

gtcgcccacc tgaagcagca ggtgagcgga ctggagggag tccaggacga tctgttctgg      420

ctgacctttg aggggaagcc cctggaagat cagctgcctc tgggagaata tggcctgaaa      480

cccctgagca ctgtctttat gaatctgagg ctgagaggcg ggggaactga acctggaggg      540

cggagc                                                                 546


<210>  8
<211>  182
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  wt ISG15 human protein

<400>  8

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Gly Trp Asp Leu Thr Val Lys Met Leu Ala Gly Asn Glu Phe 
            20                  25                  30          


Gln Val Ser Leu Ser Ser Ser Met Ser Val Ser Glu Leu Lys Ala Gln 
        35                  40                  45              


Ile Thr Gln Lys Ile Gly Val His Ala Phe Gln Gln Arg Leu Ala Val 
    50                  55                  60                  


His Pro Ser Gly Val Ala Leu Gln Asp Arg Val Pro Leu Ala Ser Gln 
65                  70                  75                  80  


Gly Leu Gly Pro Gly Ser Thr Val Leu Leu Val Val Asp Lys Cys Asp 
                85                  90                  95      


Glu Pro Leu Ser Ile Leu Val Arg Asn Asn Lys Gly Arg Ser Ser Thr 
            100                 105                 110         


Tyr Glu Val Arg Leu Thr Gln Thr Val Ala His Leu Lys Gln Gln Val 
        115                 120                 125             


Ser Gly Leu Glu Gly Val Gln Asp Asp Leu Phe Trp Leu Thr Phe Glu 
    130                 135                 140                 


Gly Lys Pro Leu Glu Asp Gln Leu Pro Leu Gly Glu Tyr Gly Leu Lys 
145                 150                 155                 160 


Pro Leu Ser Thr Val Phe Met Asn Leu Arg Leu Arg Gly Gly Gly Thr 
                165                 170                 175     


Glu Pro Gly Gly Arg Ser 
            180         


<210>  9
<211>  546
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mut ISG15 human vers.2 DNA

<400>  9
atggactgga cttggattct gttcctggtc gctgccgcaa cccgcgtgca ttctggctgg       60

gacctgactg tgaagatgct ggctggaaac gagttccagg tgtctctgag ctcctctatg      120

tctgtgagcg agctgaaggc ccagatcacc cagaagatcg gcgtgcacgc ctttcagcag      180

aggctggccg tgcaccctag cggcgtggcc ctgcaggacc gcgtgccact ggcatcccag      240

ggactgggac caggctctac cgtgctgctg gtggtggaca agtgcgatga gccactgagc      300

atcctggtgc ggaacaataa gggcagaagc tccacatacg aggtgcggct gacccagaca      360

gtggcccacc tgaagcagca ggtgtccgga ctggagggag tgcaggacga tctgttctgg      420

ctgacatttg agggcaagcc cctggaggat cagctgcctc tgggcgagta tggcctgaag      480

cccctgtcaa ccgtgtttat gaatctggct ctggcagcag caggcaccga accaggcggg      540

aggagc                                                                 546


<210>  10
<211>  182
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mut ISG15 human vers.2 protein

<400>  10

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Gly Trp Asp Leu Thr Val Lys Met Leu Ala Gly Asn Glu Phe 
            20                  25                  30          


Gln Val Ser Leu Ser Ser Ser Met Ser Val Ser Glu Leu Lys Ala Gln 
        35                  40                  45              


Ile Thr Gln Lys Ile Gly Val His Ala Phe Gln Gln Arg Leu Ala Val 
    50                  55                  60                  


His Pro Ser Gly Val Ala Leu Gln Asp Arg Val Pro Leu Ala Ser Gln 
65                  70                  75                  80  


Gly Leu Gly Pro Gly Ser Thr Val Leu Leu Val Val Asp Lys Cys Asp 
                85                  90                  95      


Glu Pro Leu Ser Ile Leu Val Arg Asn Asn Lys Gly Arg Ser Ser Thr 
            100                 105                 110         


Tyr Glu Val Arg Leu Thr Gln Thr Val Ala His Leu Lys Gln Gln Val 
        115                 120                 125             


Ser Gly Leu Glu Gly Val Gln Asp Asp Leu Phe Trp Leu Thr Phe Glu 
    130                 135                 140                 


Gly Lys Pro Leu Glu Asp Gln Leu Pro Leu Gly Glu Tyr Gly Leu Lys 
145                 150                 155                 160 


Pro Leu Ser Thr Val Phe Met Asn Leu Ala Leu Ala Ala Ala Gly Thr 
                165                 170                 175     


Glu Pro Gly Gly Arg Ser 
            180         


<210>  11
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8 HPV16 E7 epitope

<400>  11

Arg Ala His Tyr Asn Ile Val Thr Phe 
1               5                   


<210>  12
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C-terminal conjugation site for ISG15

<400>  12

Leu Arg Leu Arg Gly Gly 
1               5       


<210>  13
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutated C-terminal site for ISG15

<400>  13

Ala Ala Ala Ala Gly Gly 
1               5       


