                         SEQUENCE LISTING

<110>  THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA
       INOVIO PHARMACEUTICALS, INC.
       WEINER, David
       MORROW, Matthew
       WISE, Megan
 
<120>  VACCINES WITH CD40 AS AN ADJUVANT

<130>  VGX0139 WO

<150>  62136283
<151>  2015-03-20

<150>  62303984
<151>  2016-03-04

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  789
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human wtCD40L DNA

<400>  1
atgattgaaa cctacaacca gacctcacct cgctccgctg ccacaggact gcccattagt       60

atgaaaatct ttatgtatct gctgacagtc ttcctgatca cccagatgat tggcagcgcc      120

ctgtttgcag tgtacctgca ccggagactg gacaagatcg aggatgaacg caacctgcat      180

gaggacttcg tgtttatgaa aacaattcag cgatgcaaca ctggagaacg gtccctgtct      240

ctgctgaatt gtgaggaaat caagtcccag ttcgagggct ttgtgaagga cattatgctg      300

aacaaagagg aaacaaagaa agagaactct ttcgaaatgc agaaagggga tcagaatcct      360

cagatcgccg ctcacgtcat ttctgaggcc agctccaaga ccacaagtgt gctgcagtgg      420

gctgaaaaag gatactatac catgagtaac aatctggtca cactggaaaa cgggaagcag      480

ctgactgtga aaagacaggg actgtactat atctatgctc aggtgacctt ctgcagcaat      540

agggaggcct ctagtcaggc tccctttatc gcaagcctgt gcctgaagtc ccctggcagg      600

ttcgaacgca ttctgctgcg agcagccaac acacactcaa gcgcaaaacc ctgcggccag      660

cagtcaattc atctgggagg agtcttcgag ctgcagccag gagccagcgt gtttgtcaat      720

gtgactgatc ccagtcaggt gtcacatgga actggcttca cctcctttgg gctgctgaag      780

ctgtgatga                                                              789


<210>  2
<211>  261
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human wtCD40L protein

<400>  2

Met Ile Glu Thr Tyr Asn Gln Thr Ser Pro Arg Ser Ala Ala Thr Gly 
1               5                   10                  15      


Leu Pro Ile Ser Met Lys Ile Phe Met Tyr Leu Leu Thr Val Phe Leu 
            20                  25                  30          


Ile Thr Gln Met Ile Gly Ser Ala Leu Phe Ala Val Tyr Leu His Arg 
        35                  40                  45              


Arg Leu Asp Lys Ile Glu Asp Glu Arg Asn Leu His Glu Asp Phe Val 
    50                  55                  60                  


Phe Met Lys Thr Ile Gln Arg Cys Asn Thr Gly Glu Arg Ser Leu Ser 
65                  70                  75                  80  


Leu Leu Asn Cys Glu Glu Ile Lys Ser Gln Phe Glu Gly Phe Val Lys 
                85                  90                  95      


Asp Ile Met Leu Asn Lys Glu Glu Thr Lys Lys Glu Asn Ser Phe Glu 
            100                 105                 110         


Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser 
        115                 120                 125             


Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly 
    130                 135                 140                 


Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln 
145                 150                 155                 160 


Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr 
                165                 170                 175     


Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser 
            180                 185                 190         


Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala 
        195                 200                 205             


Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His 
    210                 215                 220                 


Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn 
225                 230                 235                 240 


Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe 
                245                 250                 255     


Gly Leu Leu Lys Leu 
            260     


<210>  3
<211>  453
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human mCD40L DNA

<400>  3
atgcagaagg gagatcagaa tccccagatc gccgctcatg tcattagcga ggctagctcc       60

aagaccacat ccgtgctgca gtgggcagaa aaaggctact atacaatgtc caacaatctg      120

gtcactctgg aaaacggaaa gcagctgacc gtgaaacgac agggcctgta ctatatctac      180

gcccaggtga cattctgcag caatagagag gcttctagtc aggcaccctt tatcgccagt      240

ctgtgcctga agtcacctgg gcggttcgaa agaattctgc tgcgggcagc caacactcac      300

tcaagcgcca aaccatgcgg gcagcagtct attcatctgg gaggggtgtt cgagctgcag      360

ccaggagcta gcgtgttcgt caatgtgacc gacccatcac aggtgtctca cgggactggc      420

ttcacatcat ttggactgct gaaactgtga tga                                   453


<210>  4
<211>  149
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human mCD40L protein

<400>  4

Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser 
1               5                   10                  15      


Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly 
            20                  25                  30          


Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln 
        35                  40                  45              


Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr 
    50                  55                  60                  


Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser 
65                  70                  75                  80  


Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala 
                85                  90                  95      


Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His 
            100                 105                 110         


Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn 
        115                 120                 125             


Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe 
    130                 135                 140                 


Gly Leu Leu Lys Leu 
145                 


<210>  5
<211>  768
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human ucCD40L DNA

<400>  5
atgattgaaa cctacaacca gacctcacct cgctccgctg ccacaggact gcccattagt       60

atgaaaatct ttatgtatct gctgacagtc ttcctgatca cccagatgat tggctccgct      120

ctgtttgcag tgtacctgca ccggagactg gacaagatcg aggatgaacg caacctgcat      180

gaggacttcg tgtttatgaa aacaattcag cgatgcaaca ctggagaacg gtccctgtct      240

ctgctgaatt gtgaggaaat caagtcccag ttcgagggct ttgtgaagga cattatgctg      300

aacaaagagg aaacaaagaa agagaacgat cagaatcctc agatcgccgc tcacgtcatt      360

tctgaggcca gctccaagac cacaagtgtg ctgcagtggg ctgaaaaagg atactatacc      420

atgagtaaca atctggtcac actggaaaac gggaagcagc tgactgtgaa aagacaggga      480

ctgtactata tctatgctca ggtgaccttc tgcagcaata gggaggcctc tagtcaggct      540

ccctttatcg caagcctgtg cctgaagtcc cctggcaggt tcgaacgcat tctgctgcga      600

gcagccaaca cacactcaag cgcaaaaccc tgcggccagc agtcaattca tctgggagga      660

gtcttcgagc tgcagccagg agccagcgtg tttgtcaatg tgactgatcc cagtcaggtg      720

tcacatggaa ctggcttcac ctcctttggg ctgctgaagc tgtgatga                   768


<210>  6
<211>  254
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human ucCD40L protein

<400>  6

Met Ile Glu Thr Tyr Asn Gln Thr Ser Pro Arg Ser Ala Ala Thr Gly 
1               5                   10                  15      


Leu Pro Ile Ser Met Lys Ile Phe Met Tyr Leu Leu Thr Val Phe Leu 
            20                  25                  30          


Ile Thr Gln Met Ile Gly Ser Ala Leu Phe Ala Val Tyr Leu His Arg 
        35                  40                  45              


Arg Leu Asp Lys Ile Glu Asp Glu Arg Asn Leu His Glu Asp Phe Val 
    50                  55                  60                  


Phe Met Lys Thr Ile Gln Arg Cys Asn Thr Gly Glu Arg Ser Leu Ser 
65                  70                  75                  80  


Leu Leu Asn Cys Glu Glu Ile Lys Ser Gln Phe Glu Gly Phe Val Lys 
                85                  90                  95      


Asp Ile Met Leu Asn Lys Glu Glu Thr Lys Lys Glu Asn Asp Gln Asn 
            100                 105                 110         


Pro Gln Ile Ala Ala His Val Ile Ser Glu Ala Ser Ser Lys Thr Thr 
        115                 120                 125             


Ser Val Leu Gln Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn Asn 
    130                 135                 140                 


Leu Val Thr Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Gln Gly 
145                 150                 155                 160 


Leu Tyr Tyr Ile Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg Glu Ala 
                165                 170                 175     


Ser Ser Gln Ala Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser Pro Gly 
            180                 185                 190         


Arg Phe Glu Arg Ile Leu Leu Arg Ala Ala Asn Thr His Ser Ser Ala 
        195                 200                 205             


Lys Pro Cys Gly Gln Gln Ser Ile His Leu Gly Gly Val Phe Glu Leu 
    210                 215                 220                 


Gln Pro Gly Ala Ser Val Phe Val Asn Val Thr Asp Pro Ser Gln Val 
225                 230                 235                 240 


Ser His Gly Thr Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu 
                245                 250                 


<210>  7
<211>  783
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mouse wtCD40L DNA

<400>  7
atgatcgaaa cttattccca gccttcccct cggtccgtcg ccacaggtct gccagcctca       60

atgaaaatct ttatgtatct cctcactgtg ttcctgatca cccagatgat tggcagcgtc      120

ctgtttgctg tgtacctgca caggagactc gacaaggtcg aggaagaagt gaatctgcat      180

gaagatttcg tgtttatcaa gaaactgaag cgctgcaaca aaggggaggg ttcactgagc      240

ctgctcaatt gtgaagagat gcggcgccag ttcgaggacc tggtgaagga tatcacactc      300

aacaaagaag agaagaaaga aaactccttc gagatgcagc gaggggacga agatccacag      360

attgccgctc acgtggtctc cgaggcaaac tctaatgcag ccagtgtgct gcagtgggcc      420

aagaaaggtt actatactat gaagagtaac ctggtcatgc tcgaaaatgg caagcagctg      480

accgtgaaaa gagagggact ctactatgtc tatacccagg tgacattctg cagcaaccga      540

gaacccagct cccagaggcc ttttatcgtg gggctgtggc tcaagccctc tagtggttcc      600

gagcggattc tgctcaaagc tgcaaataca cactcaagct cccagctgtg tgaacagcag      660

tctgtccatc tgggaggagt gttcgagctc caggcaggag cttcagtgtt tgtcaacgtg      720

actgaggcca gccaggtcat tcatcgggtg ggcttctcta gttttggact gctcaagctg      780

tag                                                                    783


<210>  8
<211>  260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse wtCD40L protein

<400>  8

Met Ile Glu Thr Tyr Ser Gln Pro Ser Pro Arg Ser Val Ala Thr Gly 
1               5                   10                  15      


Leu Pro Ala Ser Met Lys Ile Phe Met Tyr Leu Leu Thr Val Phe Leu 
            20                  25                  30          


Ile Thr Gln Met Ile Gly Ser Val Leu Phe Ala Val Tyr Leu His Arg 
        35                  40                  45              


Arg Leu Asp Lys Val Glu Glu Glu Val Asn Leu His Glu Asp Phe Val 
    50                  55                  60                  


Phe Ile Lys Lys Leu Lys Arg Cys Asn Lys Gly Glu Gly Ser Leu Ser 
65                  70                  75                  80  


Leu Leu Asn Cys Glu Glu Met Arg Arg Gln Phe Glu Asp Leu Val Lys 
                85                  90                  95      


Asp Ile Thr Leu Asn Lys Glu Glu Lys Lys Glu Asn Ser Phe Glu Met 
            100                 105                 110         


Gln Arg Gly Asp Glu Asp Pro Gln Ile Ala Ala His Val Val Ser Glu 
        115                 120                 125             


Ala Asn Ser Asn Ala Ala Ser Val Leu Gln Trp Ala Lys Lys Gly Tyr 
    130                 135                 140                 


Tyr Thr Met Lys Ser Asn Leu Val Met Leu Glu Asn Gly Lys Gln Leu 
145                 150                 155                 160 


Thr Val Lys Arg Glu Gly Leu Tyr Tyr Val Tyr Thr Gln Val Thr Phe 
                165                 170                 175     


Cys Ser Asn Arg Glu Pro Ser Ser Gln Arg Pro Phe Ile Val Gly Leu 
            180                 185                 190         


Trp Leu Lys Pro Ser Ser Gly Ser Glu Arg Ile Leu Leu Lys Ala Ala 
        195                 200                 205             


Asn Thr His Ser Ser Ser Gln Leu Cys Glu Gln Gln Ser Val His Leu 
    210                 215                 220                 


Gly Gly Val Phe Glu Leu Gln Ala Gly Ala Ser Val Phe Val Asn Val 
225                 230                 235                 240 


Thr Glu Ala Ser Gln Val Ile His Arg Val Gly Phe Ser Ser Phe Gly 
                245                 250                 255     


Leu Leu Lys Leu 
            260 


<210>  9
<211>  441
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mouse mCD40L DNA

<400>  9
atggatgaag acccccagat tgccgcacac gtcgtcagcg aagctaactc aaacgccgcc       60

tccgtcctcc agtgggctaa gaagggatac tatactatga agagtaacct ggtcatgctg      120

gagaatggga agcagctgac cgtgaaacgg gaaggtctgt actatgtcta cacccaggtg      180

acattctgct ctaacaggga gcccagctcc cagagacctt ttatcgtggg cctgtggctc      240

aagccatcta gtggcagcga acgcattctg ctcaaagccg ctaatacaca ctcaagctcc      300

cagctgtgtg agcagcagtc tgtccatctg ggcggagtgt tcgaactcca ggcaggggcc      360

tcagtgtttg tcaacgtgac cgaggctagc caggtcatcc acagggtggg gttctctagt      420

tttggtctgc tcaaactgta g                                                441


<210>  10
<211>  146
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse mCD40L protein

<400>  10

Met Asp Glu Asp Pro Gln Ile Ala Ala His Val Val Ser Glu Ala Asn 
1               5                   10                  15      


Ser Asn Ala Ala Ser Val Leu Gln Trp Ala Lys Lys Gly Tyr Tyr Thr 
            20                  25                  30          


Met Lys Ser Asn Leu Val Met Leu Glu Asn Gly Lys Gln Leu Thr Val 
        35                  40                  45              


Lys Arg Glu Gly Leu Tyr Tyr Val Tyr Thr Gln Val Thr Phe Cys Ser 
    50                  55                  60                  


Asn Arg Glu Pro Ser Ser Gln Arg Pro Phe Ile Val Gly Leu Trp Leu 
65                  70                  75                  80  


Lys Pro Ser Ser Gly Ser Glu Arg Ile Leu Leu Lys Ala Ala Asn Thr 
                85                  90                  95      


His Ser Ser Ser Gln Leu Cys Glu Gln Gln Ser Val His Leu Gly Gly 
            100                 105                 110         


Val Phe Glu Leu Gln Ala Gly Ala Ser Val Phe Val Asn Val Thr Glu 
        115                 120                 125             


Ala Ser Gln Val Ile His Arg Val Gly Phe Ser Ser Phe Gly Leu Leu 
    130                 135                 140                 


Lys Leu 
145     


<210>  11
<211>  762
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mouse ucCD40L DNA

<400>  11
atgattgaaa cttactcaca gccctcccct cggtccgtcg caactggtct gcctgcctcc       60

atgaaaatct ttatgtatct cctgactgtg ttcctgatca cccagatgat tggcagcgtc      120

ctgtttgctg tgtacctgca caggagactc gacaaggtcg aggaagaagt gaatctgcat      180

gaagatttcg tgtttatcaa gaaactgaag cgctgcaaca aaggggaggg ttcactgagc      240

ctgctcaatt gtgaagagat gcggcgccag ttcgaagacc tggtgaagga tatcacactc      300

aacaaagaag agaagaaaga aaatgacgag gacccccaga ttgccgctca cgtggtctcc      360

gaggcaaact ctaatgcagc cagtgtgctg cagtgggcca agaaagggta ctatactatg      420

aagagtaacc tggtcatgct cgaaaatggc aagcagctga ccgtgaaaag agagggactc      480

tactatgtct atacccaggt gacattctgc agcaaccgag aacccagctc ccagaggcct      540

tttatcgtgg ggctgtggct caagccctct agtggttccg agcggattct gctcaaagct      600

gcaaatacac actcaagctc ccagctgtgt gaacagcagt ctgtccatct gggaggagtg      660

ttcgagctcc aggcaggtgc ttcagtgttt gtcaacgtga ctgaggccag ccaggtcatt      720

catagagtgg gcttctctag ttttggactg ctcaagctgt ag                         762


<210>  12
<211>  253
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse ucCD40L protein

<400>  12

Met Ile Glu Thr Tyr Ser Gln Pro Ser Pro Arg Ser Val Ala Thr Gly 
1               5                   10                  15      


Leu Pro Ala Ser Met Lys Ile Phe Met Tyr Leu Leu Thr Val Phe Leu 
            20                  25                  30          


Ile Thr Gln Met Ile Gly Ser Val Leu Phe Ala Val Tyr Leu His Arg 
        35                  40                  45              


Arg Leu Asp Lys Val Glu Glu Glu Val Asn Leu His Glu Asp Phe Val 
    50                  55                  60                  


Phe Ile Lys Lys Leu Lys Arg Cys Asn Lys Gly Glu Gly Ser Leu Ser 
65                  70                  75                  80  


Leu Leu Asn Cys Glu Glu Met Arg Arg Gln Phe Glu Asp Leu Val Lys 
                85                  90                  95      


Asp Ile Thr Leu Asn Lys Glu Glu Lys Lys Glu Asn Asp Glu Asp Pro 
            100                 105                 110         


Gln Ile Ala Ala His Val Val Ser Glu Ala Asn Ser Asn Ala Ala Ser 
        115                 120                 125             


Val Leu Gln Trp Ala Lys Lys Gly Tyr Tyr Thr Met Lys Ser Asn Leu 
    130                 135                 140                 


Val Met Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Glu Gly Leu 
145                 150                 155                 160 


Tyr Tyr Val Tyr Thr Gln Val Thr Phe Cys Ser Asn Arg Glu Pro Ser 
                165                 170                 175     


Ser Gln Arg Pro Phe Ile Val Gly Leu Trp Leu Lys Pro Ser Ser Gly 
            180                 185                 190         


Ser Glu Arg Ile Leu Leu Lys Ala Ala Asn Thr His Ser Ser Ser Gln 
        195                 200                 205             


Leu Cys Glu Gln Gln Ser Val His Leu Gly Gly Val Phe Glu Leu Gln 
    210                 215                 220                 


Ala Gly Ala Ser Val Phe Val Asn Val Thr Glu Ala Ser Gln Val Ile 
225                 230                 235                 240 


His Arg Val Gly Phe Ser Ser Phe Gly Leu Leu Lys Leu 
                245                 250             


<210>  13
<211>  471
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  macaca MT CD40L DNA

<400>  13
ggatccgcca ccatgcagaa gggagaccag aaccctcaga tcgccgctca cgtcatttcc       60

gaggcttcca gcaagaccac aagcgtgctg cagtgggcag aaaaaggcta ctatacaatg      120

tctaacaatc tggtgaccct ggagaacggg aagcagctga ccgtgaaacg gcagggactc      180

tactatatct acgcccaggt gacattctgc tccaatcgcg aggcttctag tcaggcaccc      240

tttatcgcca gtctgtgcct caagtcacct gggaggttcg aaagaattct gctcagggca      300

gccaacactc actcatccgc taaaccctgc ggccagcagt ctattcatct gggcggggtc      360

ttcgaactcc agccaggagc ctccgtgttc gtcaatgtga cagatcccag ccaggtgtct      420

catggaactg gcttcaccag ctttggcctg ctcaaactgt gataactcga g               471


<210>  14
<211>  149
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  macaca MT CD40L protein

<400>  14

Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser 
1               5                   10                  15      


Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly 
            20                  25                  30          


Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln 
        35                  40                  45              


Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr 
    50                  55                  60                  


Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser 
65                  70                  75                  80  


Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala 
                85                  90                  95      


Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His 
            100                 105                 110         


Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn 
        115                 120                 125             


Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe 
    130                 135                 140                 


Gly Leu Leu Lys Leu 
145                 


