               SEQUENCE LISTING

<110> Danmarks Tekniske Universitet

<120> CRISPR/Cas-based system

<130> P3781PC00

<160> 72

<170> BiSSAP 1.3.5

<210> 1
<211> 4107
<212> DNA
<213> artificial sequence

<223> "Codon- optimised Cas9 sequence"

<220> 
<223> Codon- optimised Cas9 sequence

<220> 
<221> CDS
<222> 1..4107
<223> /note="Codon- optimised Cas9 sequence"
      /transl_table=1

<400> 1
atg gac aag aag tac tcc atc ggc ctc gac atc ggc acc aac tcc gtg      48
Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      

ggc tgg gcg gtc atc acc gac gag tac aag gtc ccc tcc aag aag ttc      96
Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          

aag gtc ctg ggc aac acc gac cgg cac tcg atc aag aag aac ctg atc     144
Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              

ggc gcc ctg ctc ttc gac agc ggc gag acc gcc gag gcg acc cgc ctg     192
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  

aag cgg acc gcg cgt cgc cgc tac acc cgg cgc aag aac cgc atc tgc     240
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  

tac ctg cag gaa atc ttc tcc aac gag atg gcc aag gtg gac gac tcg     288
Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      

ttc ttc cac cgc ctg gag gag agc ttc ctg gtg gag gag gac aag aag     336
Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         

cac gag cgc cac ccg atc ttc ggc aac atc gtg gac gag gtg gcc tac     384
His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             

cac gag aag tac ccc acc atc tac cac ctc cgc aag aag ctg gtg gac     432
His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 

tcg acc gac aag gcg gac ctg cgg ctc atc tac ctg gcc ctc gcg cac     480
Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 

atg atc aag ttc cgc ggc cac ttc ctc atc gag ggc gac ctg aac ccg     528
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     

gac aac tcc gac gtg gac aag ctc ttc atc cag ctg gtg cag acc tac     576
Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         

aac cag ctg ttc gag gag aac ccc atc aac gcc agc ggc gtg gac gcc     624
Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             

aag gcg atc ctc tcc gcg cgc ctg agc aag tcc cgg cgc ctg gag aac     672
Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 

ctc atc gcc cag ctg ccg ggc gag aag aag aac ggc ctc ttc ggc aac     720
Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 

ctg atc gcg ctg tcg ctc ggc ctg acc ccc aac ttc aag agc aac ttc     768
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     

gac ctg gcc gag gac gcg aag ctc cag ctg tcc aag gac acc tac gac     816
Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         

gac gac ctg gac aac ctg ctc gcc cag atc ggc gac cag tac gcg gac     864
Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             

ctc ttc ctg gcc gcg aag aac ctc tcg gac gcc atc ctg ctc agc gac     912
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 

atc ctg cgg gtc aac acc gag atc acc aag gcc ccg ctg tcg gcg agc     960
Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 

atg atc aag cgg tac gac gag cac cac cag gac ctg acc ctg ctc aag    1008
Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     

gcc ctc gtg cgc cag cag ctg ccc gag aag tac aag gaa atc ttc ttc    1056
Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         

gac cag tcc aag aac ggc tac gcc ggc tac atc gac ggc ggc gcg tcg    1104
Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             

cag gag gag ttc tac aag ttc atc aag ccg atc ctg gag aag atg gac    1152
Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 

ggc acc gag gag ctg ctc gtc aag ctg aac cgc gag gac ctg ctc cgc    1200
Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 

aag cag cgg acc ttc gac aac ggc tcc atc ccg cac cag atc cac ctg    1248
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     

ggc gag ctc cac gcc atc ctc cgg cgc cag gag gac ttc tac ccc ttc    1296
Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         

ctg aag gac aac cgc gag aag atc gag aag atc ctg acc ttc cgc atc    1344
Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             

ccg tac tac gtc ggc ccc ctg gcc cgc ggc aac tcc cgg ttc gcg tgg    1392
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 

atg acc cgg aag tcg gag gag acc atc acc ccg tgg aac ttc gag gag    1440
Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 

gtc gtg gac aag ggc gcg tcc gcg cag tcg ttc atc gag cgc atg acc    1488
Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     

aac ttc gac aag aac ctc ccg aac gag aag gtc ctg ccc aag cac tcc    1536
Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         

ctg ctc tac gag tac ttc acc gtg tac aac gag ctg acc aag gtc aag    1584
Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             

tac gtg acc gag ggc atg cgg aag ccg gcc ttc ctg tcg ggc gag cag    1632
Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 

aag aag gcg atc gtg gac ctg ctc ttc aag acc aac cgc aag gtc acc    1680
Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 

gtg aag cag ctg aag gag gac tac ttc aag aag atc gag tgc ttc gac    1728
Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     

tcc gtc gag atc agc ggc gtg gag gac cgc ttc aac gcc tcc ctg ggc    1776
Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         

acc tac cac gac ctg ctc aag atc atc aag gac aag gac ttc ctc gac    1824
Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             

aac gag gag aac gag gac atc ctg gag gac atc gtc ctc acc ctg acc    1872
Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 

ctc ttc gag gac cgc gag atg atc gag gag cgg ctc aag acc tac gcc    1920
Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 

cac ctg ttc gac gac aag gtg atg aag cag ctg aag cgt cgc cgc tac    1968
His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     

acc ggc tgg ggc cgc ctc tcc cgg aag ctg atc aac ggc atc cgg gac    2016
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         

aag cag agc ggc aag acc atc ctg gac ttc ctc aag tcc gac ggc ttc    2064
Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             

gcc aac cgc aac ttc atg cag ctc atc cac gac gac agc ctg acc ttc    2112
Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 

aag gag gac atc cag aag gcc cag gtc tcg ggc cag ggc gac agc ctc    2160
Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 

cac gag cac atc gcc aac ctg gcg ggc tcc ccg gcg atc aag aag ggc    2208
His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     

atc ctc cag acc gtc aag gtc gtg gac gag ctg gtc aag gtg atg ggc    2256
Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         

cgc cac aag ccc gag aac atc gtg atc gag atg gcc cgg gag aac cag    2304
Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             

acc acc cag aag ggc cag aag aac tcg cgc gag cgg atg aag cgg atc    2352
Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 

gag gag ggc atc aag gag ctc ggc agc cag atc ctg aag gag cac ccg    2400
Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 

gtc gag aac acc cag ctg cag aac gag aag ctg tac ctc tac tac ctg    2448
Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     

cag aac ggc cgc gac atg tac gtg gac cag gag ctc gac atc aac cgg    2496
Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         

ctg tcc gac tac gac gtg gac cac atc gtg ccg cag tcc ttc ctg aag    2544
Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             

gac gac tcg atc gac aac aag gtc ctg acc cgc tcg gac aag aac cgg    2592
Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 

ggc aag tcc gac aac gtg ccc tcg gag gag gtc gtg aag aag atg aag    2640
Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 

aac tac tgg cgc cag ctg ctc aac gcc aag ctc atc acc cag cgc aag    2688
Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     

ttc gac aac ctg acc aag gcc gag cgg ggc ggc ctg agc gag ctc gac    2736
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         

aag gcg ggc ttc atc aag cgc cag ctg gtc gag acc cgg cag atc acc    2784
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             

aag cac gtg gcc cag atc ctg gac tcc cgg atg aac acc aag tac gac    2832
Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 

gag aac gac aag ctg atc cgc gag gtc aag gtg atc acc ctc aag agc    2880
Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 

aag ctg gtc tcc gac ttc cgc aag gac ttc cag ttc tac aag gtc cgg    2928
Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     

gag atc aac aac tac cac cac gcc cac gac gcg tac ctg aac gcc gtc    2976
Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         

gtg ggc acc gcg ctg atc aag aag tac ccg aag ctg gag tcc gag ttc    3024
Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu Glu Ser Glu Phe 
        995                 1000                1005            

gtc tac ggc gac tac aag gtc tac gac gtg cgc aag atg atc gcc aag    3072
Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys Met Ile Ala Lys 
    1010                1015                1020                

agc gag cag gag atc ggc aag gcc acc gcg aag tac ttc ttc tac tcc    3120
Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr Phe Phe Tyr Ser 
1025                1030                1035                1040

aac atc atg aac ttc ttc aag acc gag atc acc ctg gcc aac ggc gag    3168
Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu Ala Asn Gly Glu 
                1045                1050                1055    

atc cgc aag cgg ccc ctg atc gag acc aac ggc gag acc ggc gag atc    3216
Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu Thr Gly Glu Ile 
            1060                1065                1070        

gtc tgg gac aag ggc cgc gac ttc gcc acc gtc cgg aag gtg ctg tcg    3264
Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            

atg ccg cag gtc aac atc gtg aag aag acc gag gtg cag acc ggc ggc    3312
Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly Gly 
    1090                1095                1100                

ttc agc aag gag tcc atc ctc ccc aag cgc aac agc gac aag ctg atc    3360
Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120

gcc cgg aag aag gac tgg gac ccg aag aag tac ggc ggc ttc gac agc    3408
Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    

ccc acc gtc gcc tac tcc gtg ctg gtc gtg gcg aag gtc gag aag ggc    3456
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys Val Glu Lys Gly 
            1140                1145                1150        

aag agc aag aag ctg aag tcc gtg aag gag ctg ctc ggc atc acc atc    3504
Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu Gly Ile Thr Ile 
        1155                1160                1165            

atg gag cgc tcc tcg ttc gag aag aac ccg atc gac ttc ctg gag gcc    3552
Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp Phe Leu Glu Ala 
    1170                1175                1180                

aag ggc tac aag gag gtc aag aag gac ctc atc atc aag ctg ccc aag    3600
Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200

tac agc ctg ttc gag ctg gag aac ggc cgc aag cgg atg ctc gcc tcc    3648
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg Met Leu Ala Ser 
                1205                1210                1215    

gcg ggc gag ctg cag aag ggc aac gag ctg gcc ctc ccg tcg aag tac    3696
Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu Pro Ser Lys Tyr 
            1220                1225                1230        

gtc aac ttc ctg tac ctc gcg tcc cac tac gag aag ctg aag ggc tcg    3744
Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys Leu Lys Gly Ser 
        1235                1240                1245            

ccc gag gac aac gag cag aag cag ctc ttc gtg gag cag cac aag cac    3792
Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu Gln His Lys His 
    1250                1255                1260                

tac ctg gac gag atc atc gag cag atc agc gag ttc agc aag cgc gtc    3840
Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe Ser Lys Arg Val 
1265                1270                1275                1280

atc ctg gcc gac gcg aac ctc gac aag gtg ctg tcc gcc tac aac aag    3888
Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser Ala Tyr Asn Lys 
                1285                1290                1295    

cac cgc gac aag ccg atc cgg gag cag gcg gag aac atc atc cac ctg    3936
His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn Ile Ile His Leu 
            1300                1305                1310        

ttc acc ctc acc aac ctg ggc gcc ccc gcc gcg ttc aag tac ttc gac    3984
Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe Lys Tyr Phe Asp 
        1315                1320                1325            

acc acc atc gac cgc aag cgg tac acc tcc acc aag gag gtc ctc gac    4032
Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Val Leu Asp 
    1330                1335                1340                

gcg acc ctg atc cac cag agc atc acc ggc ctg tac gag acc cgc atc    4080
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360

gac ctg tcc cag ctc ggc ggc gac tga                                4107
Asp Leu Ser Gln Leu Gly Gly Asp     
                1365                

<210> 2
<211> 1368
<212> PRT
<213> artificial sequence

<223> Synthetic Construct
<223> "[CDS]:1..4107 from SEQ ID NO 1"

<220> 
<223> Synthetic Construct
      [CDS]:1..4107 from SEQ ID NO 1

<400> 2
Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         
His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             
His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 
Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     
Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         
Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     
Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         
Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     
Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         
Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             
Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 
Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         
Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 
Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 
Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     
Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             
Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 
His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 
Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 
His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 
Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         
Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             
Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 
Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 
Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu Glu Ser Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys Met Ile Ala Lys 
    1010                1015                1020                
Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu Ala Asn Gly Glu 
                1045                1050                1055    
Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys Val Glu Lys Gly 
            1140                1145                1150        
Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp Phe Leu Glu Ala 
    1170                1175                1180                
Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg Met Leu Ala Ser 
                1205                1210                1215    
Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu Pro Ser Lys Tyr 
            1220                1225                1230        
Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys Leu Lys Gly Ser 
        1235                1240                1245            
Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu Gln His Lys His 
    1250                1255                1260                
Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe Ser Lys Arg Val 
1265                1270                1275                1280
Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser Ala Tyr Asn Lys 
                1285                1290                1295    
His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn Ile Ile His Leu 
            1300                1305                1310        
Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe Lys Tyr Phe Asp 
        1315                1320                1325            
Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Val Leu Asp 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Gln Leu Gly Gly Asp 
                1365            

<210> 3
<211> 4107
<212> DNA
<213> Streptococcus pyogenes


<220> 
<221> CDS
<222> 1..4107
<223> /note="Cas9 sequence"
      /transl_table=11

<400> 3
atg gat aag aaa tac tca ata ggc tta gat atc ggc aca aat agc gtc      48
Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      

gga tgg gcg gtg atc act gat gaa tat aag gtt ccg tct aaa aag ttc      96
Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          

aag gtt ctg gga aat aca gac cgc cac agt atc aaa aaa aat ctt ata     144
Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              

ggg gct ctt tta ttt gac agt gga gag aca gcg gaa gcg act cgt ctc     192
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  

aaa cgg aca gct cgt aga agg tat aca cgt cgg aag aat cgt att tgt     240
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  

tat cta cag gag att ttt tca aat gag atg gcg aaa gta gat gat agt     288
Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      

ttc ttt cat cga ctt gaa gag tct ttt ttg gtg gaa gaa gac aag aag     336
Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         

cat gaa cgt cat cct att ttt gga aat ata gta gat gaa gtt gct tat     384
His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             

cat gag aaa tat cca act atc tat cat ctg cga aaa aaa ttg gta gat     432
His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 

tct act gat aaa gcg gat ttg cgc tta atc tat ttg gcc tta gcg cat     480
Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 

atg att aag ttt cgt ggt cat ttt ttg att gag gga gat tta aat cct     528
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     

gat aat agt gat gtg gac aaa cta ttt atc cag ttg gta caa acc tac     576
Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         

aat caa tta ttt gaa gaa aac cct att aac gca agt gga gta gat gct     624
Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             

aaa gcg att ctt tct gca cga ttg agt aaa tca aga cga tta gaa aat     672
Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 

ctc att gct cag ctc ccc ggt gag aag aaa aat ggc tta ttt ggg aat     720
Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 

ctc att gct ttg tca ttg ggt ttg acc cct aat ttt aaa tca aat ttt     768
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     

gat ttg gca gaa gat gct aaa tta cag ctt tca aaa gat act tac gat     816
Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         

gat gat tta gat aat tta ttg gcg caa att gga gat caa tat gct gat     864
Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             

ttg ttt ttg gca gct aag aat tta tca gat gct att tta ctt tca gat     912
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 

atc cta aga gta aat act gaa ata act aag gct ccc cta tca gct tca     960
Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 

atg att aaa cgc tac gat gaa cat cat caa gac ttg act ctt tta aaa    1008
Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     

gct tta gtt cga caa caa ctt cca gaa aag tat aaa gaa atc ttt ttt    1056
Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         

gat caa tca aaa aac gga tat gca ggt tat att gat ggg gga gct agc    1104
Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             

caa gaa gaa ttt tat aaa ttt atc aaa cca att tta gaa aaa atg gat    1152
Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 

ggt act gag gaa tta ttg gtg aaa cta aat cgt gaa gat ttg ctg cgc    1200
Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 

aag caa cgg acc ttt gac aac ggc tct att ccc cat caa att cac ttg    1248
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     

ggt gag ctg cat gct att ttg aga aga caa gaa gac ttt tat cca ttt    1296
Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         

tta aaa gac aat cgt gag aag att gaa aaa atc ttg act ttt cga att    1344
Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             

cct tat tat gtt ggt cca ttg gcg cgt ggc aat agt cgt ttt gca tgg    1392
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 

atg act cgg aag tct gaa gaa aca att acc cca tgg aat ttt gaa gaa    1440
Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 

gtt gtc gat aaa ggt gct tca gct caa tca ttt att gaa cgc atg aca    1488
Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     

aac ttt gat aaa aat ctt cca aat gaa aaa gta cta cca aaa cat agt    1536
Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         

ttg ctt tat gag tat ttt acg gtt tat aac gaa ttg aca aag gtc aaa    1584
Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             

tat gtt act gaa gga atg cga aaa cca gca ttt ctt tca ggt gaa cag    1632
Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 

aag aaa gcc att gtt gat tta ctc ttc aaa aca aat cga aaa gta acc    1680
Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 

gtt aag caa tta aaa gaa gat tat ttc aaa aaa ata gaa tgt ttt gat    1728
Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     

agt gtt gaa att tca gga gtt gaa gat aga ttt aat gct tca tta ggt    1776
Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         

acc tac cat gat ttg cta aaa att att aaa gat aaa gat ttt ttg gat    1824
Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             

aat gaa gaa aat gaa gat atc tta gag gat att gtt tta aca ttg acc    1872
Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 

tta ttt gaa gat agg gag atg att gag gaa aga ctt aaa aca tat gct    1920
Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 

cac ctc ttt gat gat aag gtg atg aaa cag ctt aaa cgt cgc cgt tat    1968
His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     

act ggt tgg gga cgt ttg tct cga aaa ttg att aat ggt att agg gat    2016
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         

aag caa tct ggc aaa aca ata tta gat ttt ttg aaa tca gat ggt ttt    2064
Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             

gcc aat cgc aat ttt atg cag ctg atc cat gat gat agt ttg aca ttt    2112
Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 

aaa gaa gac att caa aaa gca caa gtg tct gga caa ggc gat agt tta    2160
Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 

cat gaa cat att gca aat tta gct ggt agc cct gct att aaa aaa ggt    2208
His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     

att tta cag act gta aaa gtt gtt gat gaa ttg gtc aaa gta atg ggg    2256
Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         

cgg cat aag cca gaa aat atc gtt att gaa atg gca cgt gaa aat cag    2304
Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             

aca act caa aag ggc cag aaa aat tcg cga gag cgt atg aaa cga atc    2352
Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 

gaa gaa ggt atc aaa gaa tta gga agt cag att ctt aaa gag cat cct    2400
Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 

gtt gaa aat act caa ttg caa aat gaa aag ctc tat ctc tat tat ctc    2448
Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     

caa aat gga aga gac atg tat gtg gac caa gaa tta gat att aat cgt    2496
Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         

tta agt gat tat gat gtc gat cac att gtt cca caa agt ttc ctt aaa    2544
Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             

gac gat tca ata gac aat aag gtc tta acg cgt tct gat aaa aat cgt    2592
Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 

ggt aaa tcg gat aac gtt cca agt gaa gaa gta gtc aaa aag atg aaa    2640
Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 

aac tat tgg aga caa ctt cta aac gcc aag tta atc act caa cgt aag    2688
Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     

ttt gat aat tta acg aaa gct gaa cgt gga ggt ttg agt gaa ctt gat    2736
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         

aaa gct ggt ttt atc aaa cgc caa ttg gtt gaa act cgc caa atc act    2784
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             

aag cat gtg gca caa att ttg gat agt cgc atg aat act aaa tac gat    2832
Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 

gaa aat gat aaa ctt att cga gag gtt aaa gtg att acc tta aaa tct    2880
Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 

aaa tta gtt tct gac ttc cga aaa gat ttc caa ttc tat aaa gta cgt    2928
Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     

gag att aac aat tac cat cat gcc cat gat gcg tat cta aat gcc gtc    2976
Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         

gtt gga act gct ttg att aag aaa tat cca aaa ctt gaa tcg gag ttt    3024
Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu Glu Ser Glu Phe 
        995                 1000                1005            

gtc tat ggt gat tat aaa gtt tat gat gtt cgt aaa atg att gct aag    3072
Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys Met Ile Ala Lys 
    1010                1015                1020                

tct gag caa gaa ata ggc aaa gca acc gca aaa tat ttc ttt tac tct    3120
Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr Phe Phe Tyr Ser 
1025                1030                1035                1040

aat atc atg aac ttc ttc aaa aca gaa att aca ctt gca aat gga gag    3168
Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu Ala Asn Gly Glu 
                1045                1050                1055    

att cgc aaa cgc cct cta atc gaa act aat ggg gaa act gga gaa att    3216
Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu Thr Gly Glu Ile 
            1060                1065                1070        

gtc tgg gat aaa ggg cga gat ttt gcc aca gtg cgc aaa gta ttg tcc    3264
Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            

atg ccc caa gtc aat att gtc aag aaa aca gaa gta cag aca ggc gga    3312
Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly Gly 
    1090                1095                1100                

ttc tcc aag gag tca att tta cca aaa aga aat tcg gac aag ctt att    3360
Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120

gct cgt aaa aaa gac tgg gat cca aaa aaa tat ggt ggt ttt gat agt    3408
Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    

cca acg gta gct tat tca gtc cta gtg gtt gct aag gtg gaa aaa ggg    3456
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys Val Glu Lys Gly 
            1140                1145                1150        

aaa tcg aag aag tta aaa tcc gtt aaa gag tta cta ggg atc aca att    3504
Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu Gly Ile Thr Ile 
        1155                1160                1165            

atg gaa aga agt tcc ttt gaa aaa aat ccg att gac ttt tta gaa gct    3552
Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp Phe Leu Glu Ala 
    1170                1175                1180                

aaa gga tat aag gaa gtt aaa aaa gac tta atc att aaa cta cct aaa    3600
Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200

tat agt ctt ttt gag tta gaa aac ggt cgt aaa cgg atg ctg gct agt    3648
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg Met Leu Ala Ser 
                1205                1210                1215    

gcc gga gaa tta caa aaa gga aat gag ctg gct ctg cca agc aaa tat    3696
Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu Pro Ser Lys Tyr 
            1220                1225                1230        

gtg aat ttt tta tat tta gct agt cat tat gaa aag ttg aag ggt agt    3744
Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys Leu Lys Gly Ser 
        1235                1240                1245            

cca gaa gat aac gaa caa aaa caa ttg ttt gtg gag cag cat aag cat    3792
Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu Gln His Lys His 
    1250                1255                1260                

tat tta gat gag att att gag caa atc agt gaa ttt tct aag cgt gtt    3840
Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe Ser Lys Arg Val 
1265                1270                1275                1280

att tta gca gat gcc aat tta gat aaa gtt ctt agt gca tat aac aaa    3888
Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser Ala Tyr Asn Lys 
                1285                1290                1295    

cat aga gac aaa cca ata cgt gaa caa gca gaa aat att att cat tta    3936
His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn Ile Ile His Leu 
            1300                1305                1310        

ttt acg ttg acg aat ctt gga gct ccc gct gct ttt aaa tat ttt gat    3984
Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe Lys Tyr Phe Asp 
        1315                1320                1325            

aca aca att gat cgt aaa cga tat acg tct aca aaa gaa gtt tta gat    4032
Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Val Leu Asp 
    1330                1335                1340                

gcc act ctt atc cat caa tcc atc act ggt ctt tat gaa aca cgc att    4080
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360

gat ttg agt cag cta gga ggt gac tga                                4107
Asp Leu Ser Gln Leu Gly Gly Asp     
                1365                

<210> 4
<211> 20
<212> DNA
<213> artificial sequence

<223> "Target sequence"

<220> 
<223> Target sequence

<220> 
<221> misc_binding
<222> 1..20
<223> /note="ActIorf1-1 NT"

<400> 4
gtggctcgaa ggaggctcga                                                20


<210> 5
<211> 20
<212> DNA
<213> artificial sequence

<223> "Target sequence"

<220> 
<223> Target sequence

<220> 
<221> misc_binding
<222> 1..20
<223> /note="ActIorf1-2 T"

<400> 5
agctcgatca agtcgatggt                                                20


<210> 6
<211> 20
<212> DNA
<213> artificial sequence

<223> "Target sequence"

<220> 
<223> Target sequence

<220> 
<221> misc_binding
<222> 1..20
<223> /note="ActIorf1-3 T"

<400> 6
gaagcgcaga gtcgtcatca                                                20


<210> 7
<211> 20
<212> DNA
<213> artificial sequence

<223> "Target sequence"

<220> 
<223> Target sequence

<220> 
<221> misc_binding
<222> 1..20
<223> /note="ActIorf1-4 T"

<400> 7
cccctcgccc taccgttcac                                                20


<210> 8
<211> 20
<212> DNA
<213> artificial sequence

<223> "Target sequence"

<220> 
<223> Target sequence

<220> 
<221> misc_binding
<222> 1..20
<223> /note="ActIorf1-5 T"

<400> 8
gcgcgagtat ctgctgctgt                                                20


<210> 9
<211> 20
<212> DNA
<213> artificial sequence

<223> "Target sequence"

<220> 
<223> Target sequence

<220> 
<221> misc_binding
<222> 1..20
<223> /note="ActIorf1-6 T"

<400> 9
ctgcaacgcg taccacatga                                                20


<210> 10
<211> 20
<212> DNA
<213> artificial sequence

<223> "Target sequence"

<220> 
<223> Target sequence

<220> 
<221> misc_binding
<222> 1..20
<223> /note="Actvb-1 NT"

<400> 10
tcgccgcaac tgtcgaacac                                                20


<210> 11
<211> 20
<212> DNA
<213> artificial sequence

<223> "Target sequence"

<220> 
<223> Target sequence

<220> 
<221> misc_binding
<222> 1..20
<223> /note="Actvb-2 NT"

<400> 11
ctgccatctt cgaactccct                                                20


<210> 12
<211> 20
<212> DNA
<213> artificial sequence

<223> "Target sequence"

<220> 
<223> Target sequence

<220> 
<221> misc_binding
<222> 1..20
<223> /note="Actvb-3 T"

<400> 12
ttcccggtgt tcgacagttg                                                20


<210> 13
<211> 20
<212> DNA
<213> artificial sequence

<223> "Target sequence"

<220> 
<223> Target sequence

<220> 
<221> misc_binding
<222> 1..20
<223> /note="Actvb-4 T"

<400> 13
actggtctgc ctggctcgta                                                20


<210> 14
<211> 20
<212> DNA
<213> artificial sequence

<223> "Target sequence"

<220> 
<223> Target sequence

<220> 
<221> misc_binding
<222> 1..20
<223> /note="Actvb-5 NT"

<400> 14
atcttcgaac tccctaggcg                                                20


<210> 15
<211> 20
<212> DNA
<213> artificial sequence

<223> "Target sequence"

<220> 
<223> Target sequence

<220> 
<221> misc_binding
<222> 1..20
<223> /note="Actvb-6 NT"

<400> 15
gtcccggagc attccctggt                                                20


<210> 16
<211> 20
<212> DNA
<213> artificial sequence

<223> "Target sequence"

<220> 
<223> Target sequence

<220> 
<221> misc_binding
<222> 1..20
<223> /note="orf1p-S1 T"

<400> 16
gtgttcccct ccctgcctcg                                                20


<210> 17
<211> 20
<212> DNA
<213> artificial sequence

<223> "Target sequence"

<220> 
<223> Target sequence

<220> 
<221> misc_binding
<222> 1..20
<223> /note="orf1p-S3 T"

<400> 17
tccctcacgc gctcagcttt                                                20


<210> 18
<211> 20
<212> DNA
<213> artificial sequence

<223> "Target sequence"

<220> 
<223> Target sequence

<220> 
<221> misc_binding
<222> 1..20
<223> /note="orf1p-S5 T"

<400> 18
ctttgggcgc ccggctcgag                                                20


<210> 19
<211> 20
<212> DNA
<213> artificial sequence

<223> "Target sequence"

<220> 
<223> Target sequence

<220> 
<221> misc_binding
<222> 1..20
<223> /note="orf1p-A1 NT"

<400> 19
ccttcgaccg ccgctcgagc                                                20


<210> 20
<211> 20
<212> DNA
<213> artificial sequence

<223> "Target sequence"

<220> 
<223> Target sequence

<220> 
<221> misc_binding
<222> 1..20
<223> /note="orf1p-A4 NT"

<400> 20
gcccaaagct gagcgcgtga                                                20


<210> 21
<211> 20
<212> DNA
<213> artificial sequence

<223> "Target sequence"

<220> 
<223> Target sequence

<220> 
<221> misc_binding
<222> 1..20
<223> /note="orf1p-A5 NT"

<400> 21
tgagcgcgtg agggaccacg                                                20


<210> 22
<211> 20
<212> DNA
<213> artificial sequence

<223> "Target sequence"

<220> 
<223> Target sequence

<220> 
<221> misc_binding
<222> 1..20
<223> /note="ActIorf1-7 NT"

<400> 22
ttcgagcctc cttcgagcca                                                20


<210> 23
<211> 20
<212> DNA
<213> artificial sequence

<223> "Target sequence"

<220> 
<223> Target sequence

<220> 
<221> misc_binding
<222> 1..20
<223> /note="ActIorf1-8 NT"

<400> 23
ggcatcgagg ggtcccgtat                                                20


<210> 24
<211> 50
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..50
<223> /note="ActIorf1-F1"

<400> 24
catgccatgg gtggctcgaa ggaggctcga gttttagagc tagaaatagc                50


<210> 25
<211> 50
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..50
<223> /note="ActIorf1-F2"

<400> 25
catgccatgg agctcgatca agtcgatggt gttttagagc tagaaatagc                50


<210> 26
<211> 50
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..50
<223> /note="ActIorf1-F3"

<400> 26
catgccatgg gaagcgcaga gtcgtcatca gttttagagc tagaaatagc                50


<210> 27
<211> 50
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..50
<223> /note="ActIorf1-F4"

<400> 27
catgccatgg cccctcgccc taccgttcac gttttagagc tagaaatagc                50


<210> 28
<211> 50
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..50
<223> /note="ActIorf1-F5"

<400> 28
catgccatgg gcgcgagtat ctgctgctgt gttttagagc tagaaatagc                50


<210> 29
<211> 50
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..50
<223> /note="ActIorf1-F6"

<400> 29
catgccatgg ctgcaacgcg taccacatga gttttagagc tagaaatagc                50


<210> 30
<211> 50
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..50
<223> /note="ActIorf1-F7"

<400> 30
catgccatgg ttcgagcctc cttcgagcca gttttagagc tagaaatagc                50


<210> 31
<211> 50
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..50
<223> /note="ActIorf1-F8"

<400> 31
catgccatgg ggcatcgagg ggtcccgtat gttttagagc tagaaatagc                50


<210> 32
<211> 50
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..50
<223> /note="ActVB-F1"

<400> 32
catgccatgg tcgccgcaac tgtcgaacac gttttagagc tagaaatagc                50


<210> 33
<211> 50
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..50
<223> /note="ActVB-F2"

<400> 33
catgccatgg ctgccatctt cgaactccct gttttagagc tagaaatagc                50


<210> 34
<211> 50
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..50
<223> /note="ActVB-F3"

<400> 34
catgccatgg ttcccggtgt tcgacagttg gttttagagc tagaaatagc                50


<210> 35
<211> 50
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..50
<223> /note="ActVB-F4"

<400> 35
catgccatgg actggtctgc ctggctcgta gttttagagc tagaaatagc                50


<210> 36
<211> 50
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..50
<223> /note="ActVB-F5"

<400> 36
catgccatgg atcttcgaac tccctaggcg gttttagagc tagaaatagc                50


<210> 37
<211> 50
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..50
<223> /note="ActVB-F6"

<400> 37
catgccatgg gtcccggagc attccctggt gttttagagc tagaaatagc                50


<210> 38
<211> 50
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..50
<223> /note="orf1p-S1 T-F"

<400> 38
catgccatgg gtgttcccct ccctgcctcg gttttagagc tagaaatagc                50


<210> 39
<211> 50
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..50
<223> /note="orf1p-S3 T-F"

<400> 39
catgccatgg tccctcacgc gctcagcttt gttttagagc tagaaatagc                50


<210> 40
<211> 50
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..50
<223> /note="orf1p-S5 T-F"

<400> 40
catgccatgg ctttgggcgc ccggctcgag gttttagagc tagaaatagc                50


<210> 41
<211> 50
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..50
<223> /note="orf1p-A1 NT-F"

<400> 41
catgccatgg ccttcgaccg ccgctcgagc gttttagagc tagaaatagc                50


<210> 42
<211> 50
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..50
<223> /note="orf1p-A4 NT-F"

<400> 42
catgccatgg gcccaaagct gagcgcgtga gttttagagc tagaaatagc                50


<210> 43
<211> 50
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..50
<223> /note="orf1p-A5 NT-F"

<400> 43
catgccatgg tgagcgcgtg agggaccacg gttttagagc tagaaatagc                50


<210> 44
<211> 33
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..33
<223> /note="sgRNA-R"

<400> 44
acgcctacgt aaaaaaagca ccgactcggt gcc                                  33


<210> 45
<211> 18
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..18
<223> /note="gRNA check-F"

<400> 45
acatgtgcgg tcgatctt                                                  18


<210> 46
<211> 20
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..20
<223> /note="gRNA check-R"

<400> 46
tacgtaaaaa aagcaccgac                                                20


<210> 47
<211> 39
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..39
<223> /note="orf1-5'F"

<400> 47
tcgtcgaagg cactagaagg catccgctga acgagaccc                            39


<210> 48
<211> 40
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..40
<223> /note="orf1-5'R"

<400> 48
gctcacgtcg aagcgggtga ccacgcagga ctccgaagtc                           40


<210> 49
<211> 19
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..19
<223> /note="orf1-3'F"

<400> 49
tcacccgctt cgacgtgag                                                 19


<210> 50
<211> 38
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..38
<223> /note="orf1-3'R"

<400> 50
ggtcgatccc cgcatatagg ttcgccgagc accaggtc                             38


<210> 51
<211> 39
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..39
<223> /note="VB-5'F"

<400> 51
tcgtcgaagg cactagaagg cgactcgctc gccctgatg                            39


<210> 52
<211> 41
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..41
<223> /note="VB-5'R"

<400> 52
caccaacctg ctcgggctgc gccgtggaag tgggtgttga c                         41


<210> 53
<211> 18
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..18
<223> /note="VB-3'F"

<400> 53
gcagcccgag caggttgg                                                  18


<210> 54
<211> 38
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..38
<223> /note="VB-3'R"

<400> 54
ggtcgatccc cgcatatagg tccgttgcgg cgtccatc                             38


<210> 55
<211> 19
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..19
<223> /note="VB-check-F"

<400> 55
cggctggtgc gtcagcaac                                                 19


<210> 56
<211> 18
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..18
<223> /note="VB-check-R"

<400> 56
acgtggcggg tcgaacgg                                                  18


<210> 57
<211> 20
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..20
<223> /note="ORF1-check-F"

<400> 57
ccgccttgag gacctgtttg                                                20


<210> 58
<211> 19
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..19
<223> /note="ORF1-check-R"

<400> 58
acacgctgac cgacttggg                                                 19


<210> 59
<211> 20
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..20
<223> /note="CAS9-check-F"

<400> 59
tccacgagca catcgccaac                                                20


<210> 60
<211> 22
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..22
<223> /note="CAS9-check-R"

<400> 60
gaccttgtag tcgccgtaga cg                                             22


<210> 61
<211> 40
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..40
<223> /note="ScaligD-F"

<400> 61
tcgtcgaagg cactagaagg gcggtcgatc ttgacggctg                           40


<210> 62
<211> 40
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..40
<223> /note="ScaligD-R"

<400> 62
ggtcgatccc cgcatatagg tgccgccggg cgttttttat                           40


<210> 63
<211> 20
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..20
<223> /note="orf1-6 ligD test-F"

<400> 63
ccgccgacac cccgatcacc                                                20


<210> 64
<211> 20
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..20
<223> /note="orf1-6 ligD test-R"

<400> 64
accgcagctt ccgctccctg                                                20


<210> 65
<211> 20
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..20
<223> /note="vb2 ligD test-F"

<400> 65
cgaggtgatc gacgccaacc                                                20


<210> 66
<211> 20
<212> DNA
<213> artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> primer_bind
<222> 1..20
<223> /note="vb2 ligD test-R"

<400> 66
tcgccgagca ggatgatgtg                                                20


<210> 67
<211> 82
<212> DNA
<213> artificial sequence

<223> "core guide RNA sequence"

<220> 
<223> core guide RNA sequence

<220> 
<221> misc_binding
<222> 1..82
<223> /note="core guide RNA"

<400> 67
gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt     60

ggcaccgagt cggtgctttt tt                                              82


<210> 68
<211> 228
<212> DNA
<213> Artificial sequence

<223> "sgRNA scaffold"

<220> 
<223> sgRNA scaffold

<220> 
<221> misc_feature
<222> 1..228
<223> /note="sgRNA scaffold; n is A, T, G or C"

<400> 68
gcggtcgatc ttgacggctg gcgagaggtg cggggaggat ctgaccgacg cggtccacac     60

gtggcaccgc gatgctgttg tgggcacaat cgtgccggtt ggtaggatcg acggccatgg    120

nnnnnnnnnn nnnnnnnnnn gttttagagc tagaaatagc aagttaaaat aaggctagtc    180

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttacgta                 228


<210> 69
<211> 50
<212> DNA
<213> Artificial sequence

<223> "Primer"

<220> 
<223> Primer

<220> 
<221> misc_binding
<222> 1..50
<223> /note="Target-specific Fw primer; n is A, T, G or C"

<220> 
<221> misc_feature
<222> 11..30
<223> /note="n is a, c, g, or t"

<400> 69
catgccatgg nnnnnnnnnn nnnnnnnnnn gttttagagc tagaaatagc                50


<210> 70
<211> 1368
<212> PRT
<213> Streptococcus pyogenes

<223> "[CDS]:1..4107 from SEQ ID NO 3"

<220> 
<223> [CDS]:1..4107 from SEQ ID NO 3

<400> 70
Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         
His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             
His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 
Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     
Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         
Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     
Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         
Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     
Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         
Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             
Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 
Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         
Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 
Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 
Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     
Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             
Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 
His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 
Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 
His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 
Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         
Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             
Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 
Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 
Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu Glu Ser Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys Met Ile Ala Lys 
    1010                1015                1020                
Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu Ala Asn Gly Glu 
                1045                1050                1055    
Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys Val Glu Lys Gly 
            1140                1145                1150        
Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp Phe Leu Glu Ala 
    1170                1175                1180                
Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg Met Leu Ala Ser 
                1205                1210                1215    
Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu Pro Ser Lys Tyr 
            1220                1225                1230        
Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys Leu Lys Gly Ser 
        1235                1240                1245            
Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu Gln His Lys His 
    1250                1255                1260                
Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe Ser Lys Arg Val 
1265                1270                1275                1280
Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser Ala Tyr Asn Lys 
                1285                1290                1295    
His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn Ile Ile His Leu 
            1300                1305                1310        
Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe Lys Tyr Phe Asp 
        1315                1320                1325            
Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Val Leu Asp 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Gln Leu Gly Gly Asp 
                1365            

<210> 71
<211> 2181
<212> DNA
<213> Streptomyces carneus

<223> "ligD"

<220> 
<223> ligD

<220> 
<221> CDS
<222> 1..2181
<223> /transl_table=1

<400> 71
atc gag gtc cgg ctg agc aac ctg gac aag gtg ctc tat ccg gcg acc      48
Ile Glu Val Arg Leu Ser Asn Leu Asp Lys Val Leu Tyr Pro Ala Thr 
1               5                   10                  15      

ggc acc acc aag ggc gag gtc atc gag tac tac gcc gaa atc gcc ccg      96
Gly Thr Thr Lys Gly Glu Val Ile Glu Tyr Tyr Ala Glu Ile Ala Pro 
            20                  25                  30          

gcg atg ctg ccg cat atc gcg ggc cgg ccg atc acc cgg aaa cgg tgg     144
Ala Met Leu Pro His Ile Ala Gly Arg Pro Ile Thr Arg Lys Arg Trp 
        35                  40                  45              

ccg aac ggt gtc gcc gaa tcg tcg ttc ttc gag aag aac ctc ggc gcg     192
Pro Asn Gly Val Ala Glu Ser Ser Phe Phe Glu Lys Asn Leu Gly Ala 
    50                  55                  60                  

ggt aca ccg tcg tgg cta ccg cgc cgt gcc cag gaa cat tcc gac cgc     240
Gly Thr Pro Ser Trp Leu Pro Arg Arg Ala Gln Glu His Ser Asp Arg 
65                  70                  75                  80  

acc gcg cac tat ccg gtg atc tcg tcg cag gcc ggc ctg gtc tgg ctg     288
Thr Ala His Tyr Pro Val Ile Ser Ser Gln Ala Gly Leu Val Trp Leu 
                85                  90                  95      

ggt cag cag gcc gcc ctg gag atc cac gta ccg caa tgg cgc ttc gac     336
Gly Gln Gln Ala Ala Leu Glu Ile His Val Pro Gln Trp Arg Phe Asp 
            100                 105                 110         

ggc gat gcg cgc gga ccc gcg acg cgg ctg gtg ttc gat ctc gat ccc     384
Gly Asp Ala Arg Gly Pro Ala Thr Arg Leu Val Phe Asp Leu Asp Pro 
        115                 120                 125             

ggc ccc ggc gcg gga ctg ccc gaa tgc gcg cgg gtg gcg ctc ggg gtg     432
Gly Pro Gly Ala Gly Leu Pro Glu Cys Ala Arg Val Ala Leu Gly Val 
    130                 135                 140                 

cgg gat atg gtc gcc gaa atc ggg atg cgc gcg ttc ccg ctg acc agc     480
Arg Asp Met Val Ala Glu Ile Gly Met Arg Ala Phe Pro Leu Thr Ser 
145                 150                 155                 160 

ggt agc aaa ggt atc cac ctg tac gtc ccg ctg gac cgg gtg ctg agc     528
Gly Ser Lys Gly Ile His Leu Tyr Val Pro Leu Asp Arg Val Leu Ser 
                165                 170                 175     

ccc ggc ggg gcg tcc acg gtg gcc aaa cag gtc gcc gcg aat ctg gag     576
Pro Gly Gly Ala Ser Thr Val Ala Lys Gln Val Ala Ala Asn Leu Glu 
            180                 185                 190         

aaa ctc ctt ccc gac ctg gtc acc gcc acc atc gcg aag agt gtg cgg     624
Lys Leu Leu Pro Asp Leu Val Thr Ala Thr Ile Ala Lys Ser Val Arg 
        195                 200                 205             

gcc ggg aag gtg ttc ctg gac tgg agt cag aac aac ccg tcc aag acg     672
Ala Gly Lys Val Phe Leu Asp Trp Ser Gln Asn Asn Pro Ser Lys Thr 
    210                 215                 220                 

acc atc gca ccg tat tcg ctg cgc ggc cgc gag cag ccg aac gtc gcc     720
Thr Ile Ala Pro Tyr Ser Leu Arg Gly Arg Glu Gln Pro Asn Val Ala 
225                 230                 235                 240 

gca cca cgc cac tgg gcg gag ctc gag gac gcc cgt gaa ctg cgg cag     768
Ala Pro Arg His Trp Ala Glu Leu Glu Asp Ala Arg Glu Leu Arg Gln 
                245                 250                 255     

ctg cgg ttc gac gaa gtt ctg gag cgt tat cgg tcc gag ggt gat ctg     816
Leu Arg Phe Asp Glu Val Leu Glu Arg Tyr Arg Ser Glu Gly Asp Leu 
            260                 265                 270         

ctg gcc ggc ctg gat aca ccc ctg aac gac gcg ttg acg aaa tac cga     864
Leu Ala Gly Leu Asp Thr Pro Leu Asn Asp Ala Leu Thr Lys Tyr Arg 
        275                 280                 285             

tcg atg cgt gac ccg gcg cgt aca ccg gag ccg gta ccg ccg cat tcg     912
Ser Met Arg Asp Pro Ala Arg Thr Pro Glu Pro Val Pro Pro His Ser 
    290                 295                 300                 

ccc cgg ccc ggc ccc ggt gac cgc tat gtc gtc cac gaa cac cac gcc     960
Pro Arg Pro Gly Pro Gly Asp Arg Tyr Val Val His Glu His His Ala 
305                 310                 315                 320 

cgg cgg ttg cac tgg gat gtg cgg ttg gaa cgc gac ggg gtg ctg gtg    1008
Arg Arg Leu His Trp Asp Val Arg Leu Glu Arg Asp Gly Val Leu Val 
                325                 330                 335     

tcg tgg gcg gtg ccc aag ggg ccg ccg gaa agc acc cgg cag aat cgg    1056
Ser Trp Ala Val Pro Lys Gly Pro Pro Glu Ser Thr Arg Gln Asn Arg 
            340                 345                 350         

ctc gcc gtg cac acc gag gac cac ccg ctg gaa tac ctg gac ttc cac    1104
Leu Ala Val His Thr Glu Asp His Pro Leu Glu Tyr Leu Asp Phe His 
        355                 360                 365             

ggc acg atc ccg gcc ggc gag tac ggg gca ggg gag ctg tcg gtc tgg    1152
Gly Thr Ile Pro Ala Gly Glu Tyr Gly Ala Gly Glu Leu Ser Val Trp 
    370                 375                 380                 

gat acc ggc acc tac cgc gcc gag aaa tgg cgc gac gac gag gtg atc    1200
Asp Thr Gly Thr Tyr Arg Ala Glu Lys Trp Arg Asp Asp Glu Val Ile 
385                 390                 395                 400 

gtg gtt ttc cgg ggc gag cgg ctc aac ggc cgg tac gcc atg atc cgg    1248
Val Val Phe Arg Gly Glu Arg Leu Asn Gly Arg Tyr Ala Met Ile Arg 
                405                 410                 415     

acc gag ggc gat caa tgg ctg atg cat ctc atg aag gac cag ccc gcg    1296
Thr Glu Gly Asp Gln Trp Leu Met His Leu Met Lys Asp Gln Pro Ala 
            420                 425                 430         

acc ggg gaa ctg ccg cgt gga ctc acc ccc atg ctg gcc acc agt ggc    1344
Thr Gly Glu Leu Pro Arg Gly Leu Thr Pro Met Leu Ala Thr Ser Gly 
        435                 440                 445             

gaa gtg gcc ggg ctg ccg gac tcg gag tgg gcg ttc gaa cgt aaa tgg    1392
Glu Val Ala Gly Leu Pro Asp Ser Glu Trp Ala Phe Glu Arg Lys Trp 
    450                 455                 460                 

gac gga tac cgg ctg ctc gtc gaa atc gat gcc ggc gaa atg cgg ctg    1440
Asp Gly Tyr Arg Leu Leu Val Glu Ile Asp Ala Gly Glu Met Arg Leu 
465                 470                 475                 480 

cgc agc cgg gcc ggt aac gac gtc acc gcg cgc tat ccc cag ttg tcg    1488
Arg Ser Arg Ala Gly Asn Asp Val Thr Ala Arg Tyr Pro Gln Leu Ser 
                485                 490                 495     

gtg ctg gcc gag gag ctg gcc gac cat cag gtg ata ctc gac ggt gag    1536
Val Leu Ala Glu Glu Leu Ala Asp His Gln Val Ile Leu Asp Gly Glu 
            500                 505                 510         

ctc atc gtc cgc ggc ccc gac ggc gcg gtg aat atc gcg ctg ttg aag    1584
Leu Ile Val Arg Gly Pro Asp Gly Ala Val Asn Ile Ala Leu Leu Lys 
        515                 520                 525             

gcg aat ccg cgg cgc gcc gaa ttc ctg gcg ttc gat ctg ctg ttc ctc    1632
Ala Asn Pro Arg Arg Ala Glu Phe Leu Ala Phe Asp Leu Leu Phe Leu 
    530                 535                 540                 

gac ggc act tca ctg ctg cgc aaa cgc tac cgc gat cgg cgg cac gtg    1680
Asp Gly Thr Ser Leu Leu Arg Lys Arg Tyr Arg Asp Arg Arg His Val 
545                 550                 555                 560 

ctc gaa gcg ctg gcc gcg acc acc acc gaa ctc cgg gtg cca ccg cgc    1728
Leu Glu Ala Leu Ala Ala Thr Thr Thr Glu Leu Arg Val Pro Pro Arg 
                565                 570                 575     

tat gag ggc gac ggc acc gag gcc ctg cac cgc agc gaa gaa gat ggc    1776
Tyr Glu Gly Asp Gly Thr Glu Ala Leu His Arg Ser Glu Glu Asp Gly 
            580                 585                 590         

gcc gag ggc gtg atc gcc aaa cgg ctg gat tcg gtg tat ctg ccc ggg    1824
Ala Glu Gly Val Ile Ala Lys Arg Leu Asp Ser Val Tyr Leu Pro Gly 
        595                 600                 605             

acc cgc ggg cat tcg tgg gtg aag cac cgg aac tgg cgt acc cag gag    1872
Thr Arg Gly His Ser Trp Val Lys His Arg Asn Trp Arg Thr Gln Glu 
    610                 615                 620                 

gtg gtg atc ggg ggt atg cgg cgc agt aag gcg cga ccg ttc gcc tcg    1920
Val Val Ile Gly Gly Met Arg Arg Ser Lys Ala Arg Pro Phe Ala Ser 
625                 630                 635                 640 

ttg ctg gtc ggg ata ccg gcc gag gac ggc ctg gtg tat gcg ggc cgg    1968
Leu Leu Val Gly Ile Pro Ala Glu Asp Gly Leu Val Tyr Ala Gly Arg 
                645                 650                 655     

gtc ggg acc ggg ttc gac gaa gcg ggg atg acc gaa ctc gcg gcc cgg    2016
Val Gly Thr Gly Phe Asp Glu Ala Gly Met Thr Glu Leu Ala Ala Arg 
            660                 665                 670         

ctg cgc cgg tcg gaa cgt aag acg ccg ccg ttc acc aac gag atg tcg    2064
Leu Arg Arg Ser Glu Arg Lys Thr Pro Pro Phe Thr Asn Glu Met Ser 
        675                 680                 685             

gcc gat gaa ctc cgg gac gcg atc tgg gtg aca ccg aag atc aaa ggc    2112
Ala Asp Glu Leu Arg Asp Ala Ile Trp Val Thr Pro Lys Ile Lys Gly 
    690                 695                 700                 

act gtt cgc tac atg gat tgg acc gac ggc gga cgc ttc tgg cat cct    2160
Thr Val Arg Tyr Met Asp Trp Thr Asp Gly Gly Arg Phe Trp His Pro 
705                 710                 715                 720 

gcc tgg ctc ggc gag gtg tga                                        2181
Ala Trp Leu Gly Glu Val     
                725         

<210> 72
<211> 726
<212> PRT
<213> Streptomyces carneus

<223> "[CDS]:1..2181 from SEQ ID NO 71"

<220> 
<223> [CDS]:1..2181 from SEQ ID NO 71

<400> 72
Ile Glu Val Arg Leu Ser Asn Leu Asp Lys Val Leu Tyr Pro Ala Thr 
1               5                   10                  15      
Gly Thr Thr Lys Gly Glu Val Ile Glu Tyr Tyr Ala Glu Ile Ala Pro 
            20                  25                  30          
Ala Met Leu Pro His Ile Ala Gly Arg Pro Ile Thr Arg Lys Arg Trp 
        35                  40                  45              
Pro Asn Gly Val Ala Glu Ser Ser Phe Phe Glu Lys Asn Leu Gly Ala 
    50                  55                  60                  
Gly Thr Pro Ser Trp Leu Pro Arg Arg Ala Gln Glu His Ser Asp Arg 
65                  70                  75                  80  
Thr Ala His Tyr Pro Val Ile Ser Ser Gln Ala Gly Leu Val Trp Leu 
                85                  90                  95      
Gly Gln Gln Ala Ala Leu Glu Ile His Val Pro Gln Trp Arg Phe Asp 
            100                 105                 110         
Gly Asp Ala Arg Gly Pro Ala Thr Arg Leu Val Phe Asp Leu Asp Pro 
        115                 120                 125             
Gly Pro Gly Ala Gly Leu Pro Glu Cys Ala Arg Val Ala Leu Gly Val 
    130                 135                 140                 
Arg Asp Met Val Ala Glu Ile Gly Met Arg Ala Phe Pro Leu Thr Ser 
145                 150                 155                 160 
Gly Ser Lys Gly Ile His Leu Tyr Val Pro Leu Asp Arg Val Leu Ser 
                165                 170                 175     
Pro Gly Gly Ala Ser Thr Val Ala Lys Gln Val Ala Ala Asn Leu Glu 
            180                 185                 190         
Lys Leu Leu Pro Asp Leu Val Thr Ala Thr Ile Ala Lys Ser Val Arg 
        195                 200                 205             
Ala Gly Lys Val Phe Leu Asp Trp Ser Gln Asn Asn Pro Ser Lys Thr 
    210                 215                 220                 
Thr Ile Ala Pro Tyr Ser Leu Arg Gly Arg Glu Gln Pro Asn Val Ala 
225                 230                 235                 240 
Ala Pro Arg His Trp Ala Glu Leu Glu Asp Ala Arg Glu Leu Arg Gln 
                245                 250                 255     
Leu Arg Phe Asp Glu Val Leu Glu Arg Tyr Arg Ser Glu Gly Asp Leu 
            260                 265                 270         
Leu Ala Gly Leu Asp Thr Pro Leu Asn Asp Ala Leu Thr Lys Tyr Arg 
        275                 280                 285             
Ser Met Arg Asp Pro Ala Arg Thr Pro Glu Pro Val Pro Pro His Ser 
    290                 295                 300                 
Pro Arg Pro Gly Pro Gly Asp Arg Tyr Val Val His Glu His His Ala 
305                 310                 315                 320 
Arg Arg Leu His Trp Asp Val Arg Leu Glu Arg Asp Gly Val Leu Val 
                325                 330                 335     
Ser Trp Ala Val Pro Lys Gly Pro Pro Glu Ser Thr Arg Gln Asn Arg 
            340                 345                 350         
Leu Ala Val His Thr Glu Asp His Pro Leu Glu Tyr Leu Asp Phe His 
        355                 360                 365             
Gly Thr Ile Pro Ala Gly Glu Tyr Gly Ala Gly Glu Leu Ser Val Trp 
    370                 375                 380                 
Asp Thr Gly Thr Tyr Arg Ala Glu Lys Trp Arg Asp Asp Glu Val Ile 
385                 390                 395                 400 
Val Val Phe Arg Gly Glu Arg Leu Asn Gly Arg Tyr Ala Met Ile Arg 
                405                 410                 415     
Thr Glu Gly Asp Gln Trp Leu Met His Leu Met Lys Asp Gln Pro Ala 
            420                 425                 430         
Thr Gly Glu Leu Pro Arg Gly Leu Thr Pro Met Leu Ala Thr Ser Gly 
        435                 440                 445             
Glu Val Ala Gly Leu Pro Asp Ser Glu Trp Ala Phe Glu Arg Lys Trp 
    450                 455                 460                 
Asp Gly Tyr Arg Leu Leu Val Glu Ile Asp Ala Gly Glu Met Arg Leu 
465                 470                 475                 480 
Arg Ser Arg Ala Gly Asn Asp Val Thr Ala Arg Tyr Pro Gln Leu Ser 
                485                 490                 495     
Val Leu Ala Glu Glu Leu Ala Asp His Gln Val Ile Leu Asp Gly Glu 
            500                 505                 510         
Leu Ile Val Arg Gly Pro Asp Gly Ala Val Asn Ile Ala Leu Leu Lys 
        515                 520                 525             
Ala Asn Pro Arg Arg Ala Glu Phe Leu Ala Phe Asp Leu Leu Phe Leu 
    530                 535                 540                 
Asp Gly Thr Ser Leu Leu Arg Lys Arg Tyr Arg Asp Arg Arg His Val 
545                 550                 555                 560 
Leu Glu Ala Leu Ala Ala Thr Thr Thr Glu Leu Arg Val Pro Pro Arg 
                565                 570                 575     
Tyr Glu Gly Asp Gly Thr Glu Ala Leu His Arg Ser Glu Glu Asp Gly 
            580                 585                 590         
Ala Glu Gly Val Ile Ala Lys Arg Leu Asp Ser Val Tyr Leu Pro Gly 
        595                 600                 605             
Thr Arg Gly His Ser Trp Val Lys His Arg Asn Trp Arg Thr Gln Glu 
    610                 615                 620                 
Val Val Ile Gly Gly Met Arg Arg Ser Lys Ala Arg Pro Phe Ala Ser 
625                 630                 635                 640 
Leu Leu Val Gly Ile Pro Ala Glu Asp Gly Leu Val Tyr Ala Gly Arg 
                645                 650                 655     
Val Gly Thr Gly Phe Asp Glu Ala Gly Met Thr Glu Leu Ala Ala Arg 
            660                 665                 670         
Leu Arg Arg Ser Glu Arg Lys Thr Pro Pro Phe Thr Asn Glu Met Ser 
        675                 680                 685             
Ala Asp Glu Leu Arg Asp Ala Ile Trp Val Thr Pro Lys Ile Lys Gly 
    690                 695                 700                 
Thr Val Arg Tyr Met Asp Trp Thr Asp Gly Gly Arg Phe Trp His Pro 
705                 710                 715                 720 
Ala Trp Leu Gly Glu Val 
                725     

