                         SEQUENCE LISTING

<110>  Longo, Michael
 
<120>  Method of Protein Purification

<130>  458202.25580

<150>  62128512
<151>  2015-03-05

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  1
atatatacat atggcaatgc agatgcagct tg                                     32


<210>  2
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  2
tatatcctag gttattagtc tttggcatct cccactcc                               38


<210>  3
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  3
agttgcccat atgaaggagg atcaagttgt gg                                     32


<210>  4
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  4
gtacaaccta ggttattact ccttcgcatc cccaattcc                              39


<210>  5
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  5
attgggcgcg cctggaaaac ctgtattttc agggatccaa agaaccgacc ctgctg           56


<210>  6
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  6
agctgcggcc gcttattagt cgaacaggtt tttaac                                 36


<210>  7
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  7
actgcaactg aattccacca acgtcgctca gagatcatac agattactac tgg              53


<210>  8
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  8
agctgcggcc gcttatcagt ctttggcatc tcccactcc                              39


<210>  9
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  9
gaataggatc caaagaaccg accctgctg                                         29


<210>  10
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  10
ggaattcagt tgcagtggta aagccagagc cagtgctgcc agtgctgcca gtgtcgaaca       60

ggtttttaac                                                              70


<210>  11
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  11
agctgcggcc gcttatcagt ctttggcatc tcccactcc                              39


<210>  12
<211>  1017
<212>  DNA
<213>  Homo sapiens

<400>  12
atggcaatgc agatgcagct tgaagcaaat gcagatactt cagtggaaga agaaagcttt       60

ggcccacaac ccatttcacg gttagagcag tgtggcataa atgccaacga tgtgaagaaa      120

ttggaagaag ctggattcca tactgtggag gctgttgcct atgcgccaaa gaaggagcta      180

ataaatatta agggaattag tgaagccaaa gctgataaaa ttctggctga ggcagctaaa      240

ttagttccaa tgggtttcac cactgcaact gaattccacc aaaggcggtc agagatcata      300

cagattacta ctggctccaa agagcttgac aaactacttc aaggtggaat tgagactgga      360

tctatcacag aaatgtttgg agaattccga actgggaaga cccagatctg tcatacgcta      420

gctgtcacct gccagcttcc cattgaccgg ggtggaggtg aaggaaaggc catgtacatt      480

gacactgagg gtacctttag gccagaacgg ctgctggcag tggctgagag gtatggtctc      540

tctggcagtg atgtcctgga taatgtagcc tatgctcgcg cgttcaacac agaccaccag      600

acccagctcc tttatcaagc atcagccatg atggtagaat ctaggtatgc actgcttatt      660

gtagacagtg ccaccgccct ttacagaaca gactactcgg gtcgaggtga gctttcagcc      720

aggcagatgc acttggccag gtttctgcgg atgcttctgc gactcgctga tgagtttggt      780

gtagcagtgg taatcactaa tcaggtggta gctcaagtgg atggagcagc gatgtttgct      840

gctgatccca aaaaacctat tggaggaaat atcatcgccc atgcatcaac aaccagattg      900

tatctgagga aaggaagagg ggaaaccaga atctgcaaaa tctacgactc tccctgtctt      960

cctgaagctg aagctatgtt cgccattaat gcagatggag tgggagatgc caaagac        1017


<210>  13
<211>  93
<212>  DNA
<213>  Homo sapiens

<400>  13
aaagaaccga ccctgctggg tttccacacc gcttccggta aaaaagttaa aatcgctaaa       60

gaatccctgg acaaagttaa aaacctgttc gac                                    93


<210>  14
<211>  765
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fusion sequence

<400>  14
ggtttcacca ctgcaactga attccaccaa aggcggtcag agatcataca gattactact       60

ggctccaaag agcttgacaa actacttcaa ggtggaattg agactggatc tatcacagaa      120

atgtttggag aattccgaac tgggaagacc cagatctgtc atacgctagc tgtcacctgc      180

cagcttccca ttgaccgggg tggaggtgaa ggaaaggcca tgtacattga cactgagggt      240

acctttaggc cagaacggct gctggcagtg gctgagaggt atggtctctc tggcagtgat      300

gtcctggata atgtagccta tgctcgcgcg ttcaacacag accaccagac ccagctcctt      360

tatcaagcat cagccatgat ggtagaatct aggtatgcac tgcttattgt agacagtgcc      420

accgcccttt acagaacaga ctactcgggt cgaggtgagc tttcagccag gcagatgcac      480

ttggccaggt ttctgcggat gcttctgcga ctcgctgatg agtttggtgt agcagtggta      540

atcactaatc aggtggtagc tcaagtggat ggagcagcga tgtttgctgc tgatcccaaa      600

aaacctattg gaggaaatat catcgcccat gcatcaacaa ccagattgta tctgaggaaa      660

ggaagagggg aaaccagaat ctgcaaaatc tacgactctc cctgtcttcc tgaagctgaa      720

gctatgttcg ccattaatgc agatggagtg ggagatgcca aagac                      765


<210>  15
<211>  1020
<212>  DNA
<213>  Homo sapiens

<400>  15
atgaaggagg atcaagttgt ggcggaagaa ccaggattcc aagatgaaga ggaatctttg       60

tttcaagata ttgacctgtt acagaaacat ggaattaacg tggctgacat taagaaactg      120

aaatcagtag gaatctgtac catcaaaggt atacagatga caacaagaag agctctatgc      180

aatgtcaaag gactctcaga agccaaagta gacaagatta aagaggcagc gaacaaacta      240

attgaaccag gattcttgac tgcatttgag tatagtgaaa agaggaaaat ggttttccat      300

atcaccaccg ggagccagga atttgataag ttactaggag gtggaattga aagtatggca      360

attacagaag cttttggaga atttcgtact ggaaaaaccc agctttctca taccctctgt      420

gtgacagctc aacttccagg agctggtggc tacccaggag gaaagattat cttcattgat      480

acagaaaata ctttccgtcc agatcgcctt agggacattg ctgatcgctt taatgtagac      540

catgatgcag tactggacaa cgtactttat gcacgtgcat atactagtga acatcagatg      600

gagctacttg attatgtagc agcaaagttc catgaagaag ctggcatctt caagctattg      660

attatcgatt caataatggc actttttcga gtggatttca gtggccgtgg ggagttggcc      720

gaacggcagc aaaaattggc ccagatgttg tcacgactcc aaaaaatctc agaagaatat      780

aacgtggctg tttttgtgac caatcaaatg actgccgatc caggagcaac tatgaccttt      840

caggcagatc ccaaaaaacc cattggggga cacattctgg ctcatgcttc aacaacaaga      900

ataagcttgc gaaagggaag aggagagctc agaattgcca agatttatga cagtcctgag      960

atgcctgaaa atgaagccac cttcgcaata actgctggag gaattgggga tgcgaaggag     1020


<210>  16
<211>  1296
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fusion sequence

<400>  16
atggacacca ttcatcacca tcaccatcac aacactagta tgaaaatcga agaaggtaaa       60

ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa      120

ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa      180

ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc      240

tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag      300

gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac      360

ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca      420

aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg      480

ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt      540

tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct      600

ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca      660

gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc      720

aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta      780

ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt      840

gacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact      900

gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct      960

tacgaggaag agttggcgaa agatccacgt attgccgcca caatggaaaa cgcccagaaa     1020

ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg     1080

gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact     1140

aattcgagct cgactagtgg atctggtggg gcgcgcctgg aaaacctgta ttttcaggga     1200

tccaaagaac cgaccctgct gggtttccac accgcttccg gtaaaaaagt taaaatcgct     1260

aaagaatccc tggacaaagt taaaaacctg ttcgac                               1296


<210>  17
<211>  2088
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fusion sequence

<400>  17
atggacacca ttcatcacca tcaccatcac aacactagta tgaaaatcga agaaggtaaa       60

ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa      120

ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa      180

ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc      240

tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag      300

gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac      360

ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca      420

aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg      480

ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt      540

tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct      600

ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca      660

gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc      720

aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta      780

ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt      840

gacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact      900

gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct      960

tacgaggaag agttggcgaa agatccacgt attgccgcca caatggaaaa cgcccagaaa     1020

ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg     1080

gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact     1140

aattcgagct cgactagtgg atctggtggg gcgcgcctgg aaaacctgta ttttcaggga     1200

tccaaagaac cgaccctgct gggtttccac accgcttccg gtaaaaaagt taaaatcgct     1260

aaagaatccc tggacaaagt taaaaacctg ttcgacactg gcagcactgg cagcactggc     1320

tctggtttca ccactgcaac tgaattccac caaaggcggt cagagatcat acagattact     1380

actggctcca aagagcttga caaactactt caaggtggaa ttgagactgg atctatcaca     1440

gaaatgtttg gagaattccg aactgggaag acccagatct gtcatacgct agctgtcacc     1500

tgccagcttc ccattgaccg gggtggaggt gaaggaaagg ccatgtacat tgacactgag     1560

ggtaccttta ggccagaacg gctgctggca gtggctgaga ggtatggtct ctctggcagt     1620

gatgtcctgg ataatgtagc ctatgctcgc gcgttcaaca cagaccacca gacccagctc     1680

ctttatcaag catcagccat gatggtagaa tctaggtatg cactgcttat tgtagacagt     1740

gccaccgccc tttacagaac agactactcg ggtcgaggtg agctttcagc caggcagatg     1800

cacttggcca ggtttctgcg gatgcttctg cgactcgctg atgagtttgg tgtagcagtg     1860

gtaatcacta atcaggtggt agctcaagtg gatggagcag cgatgtttgc tgctgatccc     1920

aaaaaaccta ttggaggaaa tatcatcgcc catgcatcaa caaccagatt gtatctgagg     1980

aaaggaagag gggaaaccag aatctgcaaa atctacgact ctccctgtct tcctgaagct     2040

gaagctatgt tcgccattaa tgcagatgga gtgggagatg ccaaagac                  2088


<210>  18
<211>  339
<212>  PRT
<213>  Homo sapiens

<400>  18

Met Ala Met Gln Met Gln Leu Glu Ala Asn Ala Asp Thr Ser Val Glu 
1               5                   10                  15      


Glu Glu Ser Phe Gly Pro Gln Pro Ile Ser Arg Leu Glu Gln Cys Gly 
            20                  25                  30          


Ile Asn Ala Asn Asp Val Lys Lys Leu Glu Glu Ala Gly Phe His Thr 
        35                  40                  45              


Val Glu Ala Val Ala Tyr Ala Pro Lys Lys Glu Leu Ile Asn Ile Lys 
    50                  55                  60                  


Gly Ile Ser Glu Ala Lys Ala Asp Lys Ile Leu Ala Glu Ala Ala Lys 
65                  70                  75                  80  


Leu Val Pro Met Gly Phe Thr Thr Ala Thr Glu Phe His Gln Arg Arg 
                85                  90                  95      


Ser Glu Ile Ile Gln Ile Thr Thr Gly Ser Lys Glu Leu Asp Lys Leu 
            100                 105                 110         


Leu Gln Gly Gly Ile Glu Thr Gly Ser Ile Thr Glu Met Phe Gly Glu 
        115                 120                 125             


Phe Arg Thr Gly Lys Thr Gln Ile Cys His Thr Leu Ala Val Thr Cys 
    130                 135                 140                 


Gln Leu Pro Ile Asp Arg Gly Gly Gly Glu Gly Lys Ala Met Tyr Ile 
145                 150                 155                 160 


Asp Thr Glu Gly Thr Phe Arg Pro Glu Arg Leu Leu Ala Val Ala Glu 
                165                 170                 175     


Arg Tyr Gly Leu Ser Gly Ser Asp Val Leu Asp Asn Val Ala Tyr Ala 
            180                 185                 190         


Arg Ala Phe Asn Thr Asp His Gln Thr Gln Leu Leu Tyr Gln Ala Ser 
        195                 200                 205             


Ala Met Met Val Glu Ser Arg Tyr Ala Leu Leu Ile Val Asp Ser Ala 
    210                 215                 220                 


Thr Ala Leu Tyr Arg Thr Asp Tyr Ser Gly Arg Gly Glu Leu Ser Ala 
225                 230                 235                 240 


Arg Gln Met His Leu Ala Arg Phe Leu Arg Met Leu Leu Arg Leu Ala 
                245                 250                 255     


Asp Glu Phe Gly Val Ala Val Val Ile Thr Asn Gln Val Val Ala Gln 
            260                 265                 270         


Val Asp Gly Ala Ala Met Phe Ala Ala Asp Pro Lys Lys Pro Ile Gly 
        275                 280                 285             


Gly Asn Ile Ile Ala His Ala Ser Thr Thr Arg Leu Tyr Leu Arg Lys 
    290                 295                 300                 


Gly Arg Gly Glu Thr Arg Ile Cys Lys Ile Tyr Asp Ser Pro Cys Leu 
305                 310                 315                 320 


Pro Glu Ala Glu Ala Met Phe Ala Ile Asn Ala Asp Gly Val Gly Asp 
                325                 330                 335     


Ala Lys Asp 
            


<210>  19
<211>  31
<212>  PRT
<213>  Homo sapiens

<400>  19

Lys Glu Pro Thr Leu Leu Gly Phe His Thr Ala Ser Gly Lys Lys Val 
1               5                   10                  15      


Lys Ile Ala Lys Glu Ser Leu Asp Lys Val Lys Asn Leu Phe Asp 
            20                  25                  30      


<210>  20
<211>  255
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion Protein

<400>  20

Gly Phe Thr Thr Ala Thr Glu Phe His Gln Arg Arg Ser Glu Ile Ile 
1               5                   10                  15      


Gln Ile Thr Thr Gly Ser Lys Glu Leu Asp Lys Leu Leu Gln Gly Gly 
            20                  25                  30          


Ile Glu Thr Gly Ser Ile Thr Glu Met Phe Gly Glu Phe Arg Thr Gly 
        35                  40                  45              


Lys Thr Gln Ile Cys His Thr Leu Ala Val Thr Cys Gln Leu Pro Ile 
    50                  55                  60                  


Asp Arg Gly Gly Gly Glu Gly Lys Ala Met Tyr Ile Asp Thr Glu Gly 
65                  70                  75                  80  


Thr Phe Arg Pro Glu Arg Leu Leu Ala Val Ala Glu Arg Tyr Gly Leu 
                85                  90                  95      


Ser Gly Ser Asp Val Leu Asp Asn Val Ala Tyr Ala Arg Ala Phe Asn 
            100                 105                 110         


Thr Asp His Gln Thr Gln Leu Leu Tyr Gln Ala Ser Ala Met Met Val 
        115                 120                 125             


Glu Ser Arg Tyr Ala Leu Leu Ile Val Asp Ser Ala Thr Ala Leu Tyr 
    130                 135                 140                 


Arg Thr Asp Tyr Ser Gly Arg Gly Glu Leu Ser Ala Arg Gln Met His 
145                 150                 155                 160 


Leu Ala Arg Phe Leu Arg Met Leu Leu Arg Leu Ala Asp Glu Phe Gly 
                165                 170                 175     


Val Ala Val Val Ile Thr Asn Gln Val Val Ala Gln Val Asp Gly Ala 
            180                 185                 190         


Ala Met Phe Ala Ala Asp Pro Lys Lys Pro Ile Gly Gly Asn Ile Ile 
        195                 200                 205             


Ala His Ala Ser Thr Thr Arg Leu Tyr Leu Arg Lys Gly Arg Gly Glu 
    210                 215                 220                 


Thr Arg Ile Cys Lys Ile Tyr Asp Ser Pro Cys Leu Pro Glu Ala Glu 
225                 230                 235                 240 


Ala Met Phe Ala Ile Asn Ala Asp Gly Val Gly Asp Ala Lys Asp 
                245                 250                 255 


<210>  21
<211>  340
<212>  PRT
<213>  Homo sapiens

<400>  21

Met Lys Glu Asp Gln Val Val Ala Glu Glu Pro Gly Phe Gln Asp Glu 
1               5                   10                  15      


Glu Glu Ser Leu Phe Gln Asp Ile Asp Leu Leu Gln Lys His Gly Ile 
            20                  25                  30          


Asn Val Ala Asp Ile Lys Lys Leu Lys Ser Val Gly Ile Cys Thr Ile 
        35                  40                  45              


Lys Gly Ile Gln Met Thr Thr Arg Arg Ala Leu Cys Asn Val Lys Gly 
    50                  55                  60                  


Leu Ser Glu Ala Lys Val Asp Lys Ile Lys Glu Ala Ala Asn Lys Leu 
65                  70                  75                  80  


Ile Glu Pro Gly Phe Leu Thr Ala Phe Glu Tyr Ser Glu Lys Arg Lys 
                85                  90                  95      


Met Val Phe His Ile Thr Thr Gly Ser Gln Glu Phe Asp Lys Leu Leu 
            100                 105                 110         


Gly Gly Gly Ile Glu Ser Met Ala Ile Thr Glu Ala Phe Gly Glu Phe 
        115                 120                 125             


Arg Thr Gly Lys Thr Gln Leu Ser His Thr Leu Cys Val Thr Ala Gln 
    130                 135                 140                 


Leu Pro Gly Ala Gly Gly Tyr Pro Gly Gly Lys Ile Ile Phe Ile Asp 
145                 150                 155                 160 


Thr Glu Asn Thr Phe Arg Pro Asp Arg Leu Arg Asp Ile Ala Asp Arg 
                165                 170                 175     


Phe Asn Val Asp His Asp Ala Val Leu Asp Asn Val Leu Tyr Ala Arg 
            180                 185                 190         


Ala Tyr Thr Ser Glu His Gln Met Glu Leu Leu Asp Tyr Val Ala Ala 
        195                 200                 205             


Lys Phe His Glu Glu Ala Gly Ile Phe Lys Leu Leu Ile Ile Asp Ser 
    210                 215                 220                 


Ile Met Ala Leu Phe Arg Val Asp Phe Ser Gly Arg Gly Glu Leu Ala 
225                 230                 235                 240 


Glu Arg Gln Gln Lys Leu Ala Gln Met Leu Ser Arg Leu Gln Lys Ile 
                245                 250                 255     


Ser Glu Glu Tyr Asn Val Ala Val Phe Val Thr Asn Gln Met Thr Ala 
            260                 265                 270         


Asp Pro Gly Ala Thr Met Thr Phe Gln Ala Asp Pro Lys Lys Pro Ile 
        275                 280                 285             


Gly Gly His Ile Leu Ala His Ala Ser Thr Thr Arg Ile Ser Leu Arg 
    290                 295                 300                 


Lys Gly Arg Gly Glu Leu Arg Ile Ala Lys Ile Tyr Asp Ser Pro Glu 
305                 310                 315                 320 


Met Pro Glu Asn Glu Ala Thr Phe Ala Ile Thr Ala Gly Gly Ile Gly 
                325                 330                 335     


Asp Ala Lys Glu 
            340 


<210>  22
<211>  432
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein

<400>  22

Met Asp Thr Ile His His His His His His Asn Thr Ser Met Lys Ile 
1               5                   10                  15      


Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys Gly Tyr Asn 
            20                  25                  30          


Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr Gly Ile Lys 
        35                  40                  45              


Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe Pro Gln Val 
    50                  55                  60                  


Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala His Asp Arg 
65                  70                  75                  80  


Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile Thr Pro Asp 
                85                  90                  95      


Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp Ala Val Arg 
            100                 105                 110         


Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu Ala Leu Ser 
        115                 120                 125             


Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys Thr Trp Glu 
    130                 135                 140                 


Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly Lys Ser Ala 
145                 150                 155                 160 


Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro Leu Ile Ala 
                165                 170                 175     


Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys Tyr Asp Ile 
            180                 185                 190         


Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly Leu Thr Phe 
        195                 200                 205             


Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp Thr Asp Tyr 
    210                 215                 220                 


Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala Met Thr Ile 
225                 230                 235                 240 


Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys Val Asn Tyr 
                245                 250                 255     


Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser Lys Pro Phe 
            260                 265                 270         


Val Gly Val Leu Ser Ala Gly Ile Asp Ala Ala Ser Pro Asn Lys Glu 
        275                 280                 285             


Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp Glu Gly Leu 
    290                 295                 300                 


Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala Leu Lys Ser 
305                 310                 315                 320 


Tyr Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala Thr Met Glu 
                325                 330                 335     


Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln Met Ser Ala 
            340                 345                 350         


Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala Ser Gly Arg 
        355                 360                 365             


Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn Ser Ser Ser 
    370                 375                 380                 


Thr Ser Gly Ser Gly Gly Ala Arg Leu Glu Asn Leu Tyr Phe Gln Gly 
385                 390                 395                 400 


Ser Lys Glu Pro Thr Leu Leu Gly Phe His Thr Ala Ser Gly Lys Lys 
                405                 410                 415     


Val Lys Ile Ala Lys Glu Ser Leu Asp Lys Val Lys Asn Leu Phe Asp 
            420                 425                 430         


<210>  23
<211>  696
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein

<400>  23

Met Asp Thr Ile His His His His His His Asn Thr Ser Met Lys Ile 
1               5                   10                  15      


Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys Gly Tyr Asn 
            20                  25                  30          


Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr Gly Ile Lys 
        35                  40                  45              


Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe Pro Gln Val 
    50                  55                  60                  


Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala His Asp Arg 
65                  70                  75                  80  


Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile Thr Pro Asp 
                85                  90                  95      


Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp Ala Val Arg 
            100                 105                 110         


Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu Ala Leu Ser 
        115                 120                 125             


Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys Thr Trp Glu 
    130                 135                 140                 


Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly Lys Ser Ala 
145                 150                 155                 160 


Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro Leu Ile Ala 
                165                 170                 175     


Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys Tyr Asp Ile 
            180                 185                 190         


Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly Leu Thr Phe 
        195                 200                 205             


Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp Thr Asp Tyr 
    210                 215                 220                 


Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala Met Thr Ile 
225                 230                 235                 240 


Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys Val Asn Tyr 
                245                 250                 255     


Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser Lys Pro Phe 
            260                 265                 270         


Val Gly Val Leu Ser Ala Gly Ile Asp Ala Ala Ser Pro Asn Lys Glu 
        275                 280                 285             


Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp Glu Gly Leu 
    290                 295                 300                 


Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala Leu Lys Ser 
305                 310                 315                 320 


Tyr Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala Thr Met Glu 
                325                 330                 335     


Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln Met Ser Ala 
            340                 345                 350         


Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala Ser Gly Arg 
        355                 360                 365             


Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn Ser Ser Ser 
    370                 375                 380                 


Thr Ser Gly Ser Gly Gly Ala Arg Leu Glu Asn Leu Tyr Phe Gln Gly 
385                 390                 395                 400 


Ser Lys Glu Pro Thr Leu Leu Gly Phe His Thr Ala Ser Gly Lys Lys 
                405                 410                 415     


Val Lys Ile Ala Lys Glu Ser Leu Asp Lys Val Lys Asn Leu Phe Asp 
            420                 425                 430         


Thr Gly Ser Thr Gly Ser Thr Gly Ser Gly Phe Thr Thr Ala Thr Glu 
        435                 440                 445             


Phe His Gln Arg Arg Ser Glu Ile Ile Gln Ile Thr Thr Gly Ser Lys 
    450                 455                 460                 


Glu Leu Asp Lys Leu Leu Gln Gly Gly Ile Glu Thr Gly Ser Ile Thr 
465                 470                 475                 480 


Glu Met Phe Gly Glu Phe Arg Thr Gly Lys Thr Gln Ile Cys His Thr 
                485                 490                 495     


Leu Ala Val Thr Cys Gln Leu Pro Ile Asp Arg Gly Gly Gly Glu Gly 
            500                 505                 510         


Lys Ala Met Tyr Ile Asp Thr Glu Gly Thr Phe Arg Pro Glu Arg Leu 
        515                 520                 525             


Leu Ala Val Ala Glu Arg Tyr Gly Leu Ser Gly Ser Asp Val Leu Asp 
    530                 535                 540                 


Asn Val Ala Tyr Ala Arg Ala Phe Asn Thr Asp His Gln Thr Gln Leu 
545                 550                 555                 560 


Leu Tyr Gln Ala Ser Ala Met Met Val Glu Ser Arg Tyr Ala Leu Leu 
                565                 570                 575     


Ile Val Asp Ser Ala Thr Ala Leu Tyr Arg Thr Asp Tyr Ser Gly Arg 
            580                 585                 590         


Gly Glu Leu Ser Ala Arg Gln Met His Leu Ala Arg Phe Leu Arg Met 
        595                 600                 605             


Leu Leu Arg Leu Ala Asp Glu Phe Gly Val Ala Val Val Ile Thr Asn 
    610                 615                 620                 


Gln Val Val Ala Gln Val Asp Gly Ala Ala Met Phe Ala Ala Asp Pro 
625                 630                 635                 640 


Lys Lys Pro Ile Gly Gly Asn Ile Ile Ala His Ala Ser Thr Thr Arg 
                645                 650                 655     


Leu Tyr Leu Arg Lys Gly Arg Gly Glu Thr Arg Ile Cys Lys Ile Tyr 
            660                 665                 670         


Asp Ser Pro Cys Leu Pro Glu Ala Glu Ala Met Phe Ala Ile Asn Ala 
        675                 680                 685             


Asp Gly Val Gly Asp Ala Lys Asp 
    690                 695     


<210>  24
<211>  597
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pRSFDuet-1 plasmid multiple cloning site

<400>  24
gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt       60

atgcgactcc tgcattagga aattaatacg actcactata ggggaattgt gagcggataa      120

caattcccct gtagaaataa ttttgtttaa ctttaataag gagatatacc atgggcagca      180

gccatcacca tcatcaccac agccaggatc cgaattcgag ctcggcgcgc ctgcaggtcg      240

acaagcttgc ggccgcataa tgcttaagtc gaacagaaag taatcgtatt gtacacggcc      300

gcataatcga aattaatacg actcactata ggggaattgt gagcggataa caattcccca      360

tcttagtata ttagttaagt ataagaagga gatatacata tggcagatct caattggata      420

tcggccggcc acgcgatcgc tgacgtcggt accctcgagt ctggtaaaga aaccgctgct      480

gcgaaatttg aacgccagca catggactcg tctactagcg cagcttaatt aacctaggct      540

gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttg         597


