                         SEQUENCE LISTING

<110>  Radiometer Medical ApS
 
<120>  Modified creatinase

<130>  P259

<160>  48    

<170>  PatentIn version 3.5

<210>  1
<211>  1215
<212>  DNA
<213>  Alcaligenes strain:KS-85


<220>
<221>  CDS
<222>  (1)..(1215)
<223>  AB016788.1 Alcaligenes sp. strain:KS-85. gene encoding creatine 
       amidinohydrolase [Q9RHU9]

<400>  1
atg act gac gac atg ttg cac gtg atg aaa tgg cac aac ggc gaa aaa         48
Met Thr Asp Asp Met Leu His Val Met Lys Trp His Asn Gly Glu Lys           
1               5                   10                  15                

gat tat tcg ccg ttt tcg gat gcc gag atg acc cgc cgc caa aac gac         96
Asp Tyr Ser Pro Phe Ser Asp Ala Glu Met Thr Arg Arg Gln Asn Asp           
            20                  25                  30                    

gtt cgc ggc tgg atg gcc aag aac aat gtc gat gcg gcg ctg ttc acc        144
Val Arg Gly Trp Met Ala Lys Asn Asn Val Asp Ala Ala Leu Phe Thr           
        35                  40                  45                        

tct tat cac tgc atc aac tac tat tcc ggc tgg ctg tac tgc tat ttc        192
Ser Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp Leu Tyr Cys Tyr Phe           
    50                  55                  60                            

gga cgc aag tac ggc atg gtc atc gac cac aac aac gcc acg acg att        240
Gly Arg Lys Tyr Gly Met Val Ile Asp His Asn Asn Ala Thr Thr Ile           
65                  70                  75                  80            

tcg gcc ggc atc gac ggc ggc cag ccc tgg cgc cgc agc ttc ggc gac        288
Ser Ala Gly Ile Asp Gly Gly Gln Pro Trp Arg Arg Ser Phe Gly Asp           
                85                  90                  95                

aac atc acc tac acc gac tgg cgc cgc gac aat ttc tat cgc gcc gtg        336
Asn Ile Thr Tyr Thr Asp Trp Arg Arg Asp Asn Phe Tyr Arg Ala Val           
            100                 105                 110                   

cgc cag ctg acc acg ggc gcc aag cgc atc ggc atc gag ttc gac cac        384
Arg Gln Leu Thr Thr Gly Ala Lys Arg Ile Gly Ile Glu Phe Asp His           
        115                 120                 125                       

gtc aat ctc gac ttc cgc cgc cag ctc gag gaa gcc cta ccg ggc gtc        432
Val Asn Leu Asp Phe Arg Arg Gln Leu Glu Glu Ala Leu Pro Gly Val           
    130                 135                 140                           

gag ttc gtc gac atc agc cag ccc tcg atg tgg atg cgc acc atc aag        480
Glu Phe Val Asp Ile Ser Gln Pro Ser Met Trp Met Arg Thr Ile Lys           
145                 150                 155                 160           

tcg ctc gaa gag cag aag ctg atc cgc gaa ggc gcc cgc gtg tgt gac        528
Ser Leu Glu Glu Gln Lys Leu Ile Arg Glu Gly Ala Arg Val Cys Asp           
                165                 170                 175               

gtc ggc ggc gcg gcc tgc gcg gct gcc atc aag gcc ggc gtg ccc gag        576
Val Gly Gly Ala Ala Cys Ala Ala Ala Ile Lys Ala Gly Val Pro Glu           
            180                 185                 190                   

cat gaa gtg gcg atc gcc acc acc aat gcg atg atc cgc gag atc gcc        624
His Glu Val Ala Ile Ala Thr Thr Asn Ala Met Ile Arg Glu Ile Ala           
        195                 200                 205                       

aaa tcg ttc ccc ttc gtg gag ctg atg gac acc tgg acc tgg ttc cag        672
Lys Ser Phe Pro Phe Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln           
    210                 215                 220                           

tcg ggc atc aac acc gac ggc gcg cac aat ccg gtc acc aac cgc atc        720
Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro Val Thr Asn Arg Ile           
225                 230                 235                 240           

gtg caa tcc ggc gac atc ctt tcg ctc aac acc ttc ccg atg atc ttc        768
Val Gln Ser Gly Asp Ile Leu Ser Leu Asn Thr Phe Pro Met Ile Phe           
                245                 250                 255               

ggc tac tac acc gcg ctg gag cgc acg ctg ttc tgc gac cat gtc gat        816
Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Leu Phe Cys Asp His Val Asp           
            260                 265                 270                   

gac gcc agc ctc gac atc tgg gag aag aac gtg gcc gtg cat cgc cgc        864
Asp Ala Ser Leu Asp Ile Trp Glu Lys Asn Val Ala Val His Arg Arg           
        275                 280                 285                       

ggg ctc gag ctg atc aag ccg ggc gcg cgc tgc aag gac atc gcc atc        912
Gly Leu Glu Leu Ile Lys Pro Gly Ala Arg Cys Lys Asp Ile Ala Ile           
    290                 295                 300                           

gag ctc aac gag atg tac cgc gag tgg gac ctg ctg aag tac cgc tcc        960
Glu Leu Asn Glu Met Tyr Arg Glu Trp Asp Leu Leu Lys Tyr Arg Ser           
305                 310                 315                 320           

ttc ggc tat ggc cac tcc ttc ggc gtg ctg tgc cac tac tac ggt cgc       1008
Phe Gly Tyr Gly His Ser Phe Gly Val Leu Cys His Tyr Tyr Gly Arg           
                325                 330                 335               

gag gcg ggc gtg gag ctg cgc gag gac atc gac acc gag ctg aag ccc       1056
Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Asp Thr Glu Leu Lys Pro           
            340                 345                 350                   

ggc atg gtg gtc tcc atg gag ccg atg gtg atg ctg ccg gag ggc atg       1104
Gly Met Val Val Ser Met Glu Pro Met Val Met Leu Pro Glu Gly Met           
        355                 360                 365                       

ccc ggt gcc ggc ggc tat cgc gag cac gac atc ctg atc gtc ggg gag       1152
Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile Leu Ile Val Gly Glu           
    370                 375                 380                           

gac ggt gcc gag aac atc acc ggc ttc ccg ttc ggt ccg gaa cac aac       1200
Asp Gly Ala Glu Asn Ile Thr Gly Phe Pro Phe Gly Pro Glu His Asn           
385                 390                 395                 400           

atc atc cgc aac tga                                                   1215
Ile Ile Arg Asn                                                           
                                                                          


<210>  2
<211>  404
<212>  PRT
<213>  Alcaligenes strain:KS-85

<400>  2

Met Thr Asp Asp Met Leu His Val Met Lys Trp His Asn Gly Glu Lys 
1               5                   10                  15      


Asp Tyr Ser Pro Phe Ser Asp Ala Glu Met Thr Arg Arg Gln Asn Asp 
            20                  25                  30          


Val Arg Gly Trp Met Ala Lys Asn Asn Val Asp Ala Ala Leu Phe Thr 
        35                  40                  45              


Ser Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp Leu Tyr Cys Tyr Phe 
    50                  55                  60                  


Gly Arg Lys Tyr Gly Met Val Ile Asp His Asn Asn Ala Thr Thr Ile 
65                  70                  75                  80  


Ser Ala Gly Ile Asp Gly Gly Gln Pro Trp Arg Arg Ser Phe Gly Asp 
                85                  90                  95      


Asn Ile Thr Tyr Thr Asp Trp Arg Arg Asp Asn Phe Tyr Arg Ala Val 
            100                 105                 110         


Arg Gln Leu Thr Thr Gly Ala Lys Arg Ile Gly Ile Glu Phe Asp His 
        115                 120                 125             


Val Asn Leu Asp Phe Arg Arg Gln Leu Glu Glu Ala Leu Pro Gly Val 
    130                 135                 140                 


Glu Phe Val Asp Ile Ser Gln Pro Ser Met Trp Met Arg Thr Ile Lys 
145                 150                 155                 160 


Ser Leu Glu Glu Gln Lys Leu Ile Arg Glu Gly Ala Arg Val Cys Asp 
                165                 170                 175     


Val Gly Gly Ala Ala Cys Ala Ala Ala Ile Lys Ala Gly Val Pro Glu 
            180                 185                 190         


His Glu Val Ala Ile Ala Thr Thr Asn Ala Met Ile Arg Glu Ile Ala 
        195                 200                 205             


Lys Ser Phe Pro Phe Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln 
    210                 215                 220                 


Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro Val Thr Asn Arg Ile 
225                 230                 235                 240 


Val Gln Ser Gly Asp Ile Leu Ser Leu Asn Thr Phe Pro Met Ile Phe 
                245                 250                 255     


Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Leu Phe Cys Asp His Val Asp 
            260                 265                 270         


Asp Ala Ser Leu Asp Ile Trp Glu Lys Asn Val Ala Val His Arg Arg 
        275                 280                 285             


Gly Leu Glu Leu Ile Lys Pro Gly Ala Arg Cys Lys Asp Ile Ala Ile 
    290                 295                 300                 


Glu Leu Asn Glu Met Tyr Arg Glu Trp Asp Leu Leu Lys Tyr Arg Ser 
305                 310                 315                 320 


Phe Gly Tyr Gly His Ser Phe Gly Val Leu Cys His Tyr Tyr Gly Arg 
                325                 330                 335     


Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Asp Thr Glu Leu Lys Pro 
            340                 345                 350         


Gly Met Val Val Ser Met Glu Pro Met Val Met Leu Pro Glu Gly Met 
        355                 360                 365             


Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile Leu Ile Val Gly Glu 
    370                 375                 380                 


Asp Gly Ala Glu Asn Ile Thr Gly Phe Pro Phe Gly Pro Glu His Asn 
385                 390                 395                 400 


Ile Ile Arg Asn 
                


<210>  3
<211>  404
<212>  PRT
<213>  Alcaligenes sp.


<220>
<221>  mat_peptide
<222>  (1)..(404)
<223>  Mutant creatine amidinohydrolase _Alcaligenes sp. Q9RHU9 with 
       C175A+C299A substitutions

<400>  3

Met Thr Asp Asp Met Leu His Val Met Lys Trp His Asn Gly Glu Lys 
1               5                   10                  15      


Asp Tyr Ser Pro Phe Ser Asp Ala Glu Met Thr Arg Arg Gln Asn Asp 
            20                  25                  30          


Val Arg Gly Trp Met Ala Lys Asn Asn Val Asp Ala Ala Leu Phe Thr 
        35                  40                  45              


Ser Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp Leu Tyr Cys Tyr Phe 
    50                  55                  60                  


Gly Arg Lys Tyr Gly Met Val Ile Asp His Asn Asn Ala Thr Thr Ile 
65                  70                  75                  80  


Ser Ala Gly Ile Asp Gly Gly Gln Pro Trp Arg Arg Ser Phe Gly Asp 
                85                  90                  95      


Asn Ile Thr Tyr Thr Asp Trp Arg Arg Asp Asn Phe Tyr Arg Ala Val 
            100                 105                 110         


Arg Gln Leu Thr Thr Gly Ala Lys Arg Ile Gly Ile Glu Phe Asp His 
        115                 120                 125             


Val Asn Leu Asp Phe Arg Arg Gln Leu Glu Glu Ala Leu Pro Gly Val 
    130                 135                 140                 


Glu Phe Val Asp Ile Ser Gln Pro Ser Met Trp Met Arg Thr Ile Lys 
145                 150                 155                 160 


Ser Leu Glu Glu Gln Lys Leu Ile Arg Glu Gly Ala Arg Val Ala Asp 
                165                 170                 175     


Val Gly Gly Ala Ala Cys Ala Ala Ala Ile Lys Ala Gly Val Pro Glu 
            180                 185                 190         


His Glu Val Ala Ile Ala Thr Thr Asn Ala Met Ile Arg Glu Ile Ala 
        195                 200                 205             


Lys Ser Phe Pro Phe Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln 
    210                 215                 220                 


Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro Val Thr Asn Arg Ile 
225                 230                 235                 240 


Val Gln Ser Gly Asp Ile Leu Ser Leu Asn Thr Phe Pro Met Ile Phe 
                245                 250                 255     


Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Leu Phe Cys Asp His Val Asp 
            260                 265                 270         


Asp Ala Ser Leu Asp Ile Trp Glu Lys Asn Val Ala Val His Arg Arg 
        275                 280                 285             


Gly Leu Glu Leu Ile Lys Pro Gly Ala Arg Ala Lys Asp Ile Ala Ile 
    290                 295                 300                 


Glu Leu Asn Glu Met Tyr Arg Glu Trp Asp Leu Leu Lys Tyr Arg Ser 
305                 310                 315                 320 


Phe Gly Tyr Gly His Ser Phe Gly Val Leu Cys His Tyr Tyr Gly Arg 
                325                 330                 335     


Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Asp Thr Glu Leu Lys Pro 
            340                 345                 350         


Gly Met Val Val Ser Met Glu Pro Met Val Met Leu Pro Glu Gly Met 
        355                 360                 365             


Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile Leu Ile Val Gly Glu 
    370                 375                 380                 


Asp Gly Ala Glu Asn Ile Thr Gly Phe Pro Phe Gly Pro Glu His Asn 
385                 390                 395                 400 


Ile Ile Arg Asn 
                


<210>  4
<211>  404
<212>  PRT
<213>  Alcaligenes sp.


<220>
<221>  mat_peptide
<222>  (1)..(404)
<223>  Mutant creatine amidinohydrolase _Alcaligenes sp. Q9RHU9 with 
       C175A+C299A+C268L substitutions

<400>  4

Met Thr Asp Asp Met Leu His Val Met Lys Trp His Asn Gly Glu Lys 
1               5                   10                  15      


Asp Tyr Ser Pro Phe Ser Asp Ala Glu Met Thr Arg Arg Gln Asn Asp 
            20                  25                  30          


Val Arg Gly Trp Met Ala Lys Asn Asn Val Asp Ala Ala Leu Phe Thr 
        35                  40                  45              


Ser Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp Leu Tyr Cys Tyr Phe 
    50                  55                  60                  


Gly Arg Lys Tyr Gly Met Val Ile Asp His Asn Asn Ala Thr Thr Ile 
65                  70                  75                  80  


Ser Ala Gly Ile Asp Gly Gly Gln Pro Trp Arg Arg Ser Phe Gly Asp 
                85                  90                  95      


Asn Ile Thr Tyr Thr Asp Trp Arg Arg Asp Asn Phe Tyr Arg Ala Val 
            100                 105                 110         


Arg Gln Leu Thr Thr Gly Ala Lys Arg Ile Gly Ile Glu Phe Asp His 
        115                 120                 125             


Val Asn Leu Asp Phe Arg Arg Gln Leu Glu Glu Ala Leu Pro Gly Val 
    130                 135                 140                 


Glu Phe Val Asp Ile Ser Gln Pro Ser Met Trp Met Arg Thr Ile Lys 
145                 150                 155                 160 


Ser Leu Glu Glu Gln Lys Leu Ile Arg Glu Gly Ala Arg Val Ala Asp 
                165                 170                 175     


Val Gly Gly Ala Ala Cys Ala Ala Ala Ile Lys Ala Gly Val Pro Glu 
            180                 185                 190         


His Glu Val Ala Ile Ala Thr Thr Asn Ala Met Ile Arg Glu Ile Ala 
        195                 200                 205             


Lys Ser Phe Pro Phe Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln 
    210                 215                 220                 


Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro Val Thr Asn Arg Ile 
225                 230                 235                 240 


Val Gln Ser Gly Asp Ile Leu Ser Leu Asn Thr Phe Pro Met Ile Phe 
                245                 250                 255     


Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Leu Phe Leu Asp His Val Asp 
            260                 265                 270         


Asp Ala Ser Leu Asp Ile Trp Glu Lys Asn Val Ala Val His Arg Arg 
        275                 280                 285             


Gly Leu Glu Leu Ile Lys Pro Gly Ala Arg Ala Lys Asp Ile Ala Ile 
    290                 295                 300                 


Glu Leu Asn Glu Met Tyr Arg Glu Trp Asp Leu Leu Lys Tyr Arg Ser 
305                 310                 315                 320 


Phe Gly Tyr Gly His Ser Phe Gly Val Leu Cys His Tyr Tyr Gly Arg 
                325                 330                 335     


Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Asp Thr Glu Leu Lys Pro 
            340                 345                 350         


Gly Met Val Val Ser Met Glu Pro Met Val Met Leu Pro Glu Gly Met 
        355                 360                 365             


Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile Leu Ile Val Gly Glu 
    370                 375                 380                 


Asp Gly Ala Glu Asn Ile Thr Gly Phe Pro Phe Gly Pro Glu His Asn 
385                 390                 395                 400 


Ile Ile Arg Asn 
                


<210>  5
<211>  1203
<212>  DNA
<213>  Ochrobactrum anthropi


<220>
<221>  CDS
<222>  (1)..(1203)
<223>  AIK40910.1 Ochrobactrum anthropi gene encoding creatine 
       amidinohydrolase [A0A076WGB5]

<400>  5
atg ctg cat gtc atg aaa tgg cac aat ggc gag aag gac tat tcg ccg         48
Met Leu His Val Met Lys Trp His Asn Gly Glu Lys Asp Tyr Ser Pro           
1               5                   10                  15                

ttc tcc gag gcc gaa atg acc cgc cgt cag aac gac gtg cgg ggc tgg         96
Phe Ser Glu Ala Glu Met Thr Arg Arg Gln Asn Asp Val Arg Gly Trp           
            20                  25                  30                    

atg gcc aag aac gac gtc gat gcg gcg ctg ttc acc tcc tac cat tgc        144
Met Ala Lys Asn Asp Val Asp Ala Ala Leu Phe Thr Ser Tyr His Cys           
        35                  40                  45                        

atc aac tat tat tcg ggt tgg ctg tac tgc tat ttc ggc cgc aaa tac        192
Ile Asn Tyr Tyr Ser Gly Trp Leu Tyr Cys Tyr Phe Gly Arg Lys Tyr           
    50                  55                  60                            

ggc atg gtc atc gac cac aac aag gcg acc acc atc tcc gcc ggc atc        240
Gly Met Val Ile Asp His Asn Lys Ala Thr Thr Ile Ser Ala Gly Ile           
65                  70                  75                  80            

gat ggc ggc cag ccc tgg cgg cgc agc ttc ggc gac aac atc acc tat        288
Asp Gly Gly Gln Pro Trp Arg Arg Ser Phe Gly Asp Asn Ile Thr Tyr           
                85                  90                  95                

acc gac tgg cgc cgg gac aat ttc tat cag gca gtg cgc cag ttg acg        336
Thr Asp Trp Arg Arg Asp Asn Phe Tyr Gln Ala Val Arg Gln Leu Thr           
            100                 105                 110                   

aag ggc gcc aag cgc gtc ggc atc gaa ttc gat cat gtc tcg ctc gac        384
Lys Gly Ala Lys Arg Val Gly Ile Glu Phe Asp His Val Ser Leu Asp           
        115                 120                 125                       

ttc cgc cgc cag ctc gag gaa gcg ctg ccg ggc gtc gag ttc gtg gat        432
Phe Arg Arg Gln Leu Glu Glu Ala Leu Pro Gly Val Glu Phe Val Asp           
    130                 135                 140                           

gtc ggc cag ccc tcg atg tgg atg cgc acg atc aag tcg gcg gag gag        480
Val Gly Gln Pro Ser Met Trp Met Arg Thr Ile Lys Ser Ala Glu Glu           
145                 150                 155                 160           

cag aag ctg atc cgc gaa gga gcg cgc gtg tgt gac gta ggc ggt gcg        528
Gln Lys Leu Ile Arg Glu Gly Ala Arg Val Cys Asp Val Gly Gly Ala           
                165                 170                 175               

gcc tgc gcg gcg gcg gtc aag gct ggc gta ccg gaa cat gaa gtc gcc        576
Ala Cys Ala Ala Ala Val Lys Ala Gly Val Pro Glu His Glu Val Ala           
            180                 185                 190                   

atc gcc acc acc aat gca atg gtc cgc gag atc gcc aag tcg ttt ccg        624
Ile Ala Thr Thr Asn Ala Met Val Arg Glu Ile Ala Lys Ser Phe Pro           
        195                 200                 205                       

ttc gtc gag ctg atg gac acc tgg acc tgg ttc cag tcg ggc atc aac        672
Phe Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln Ser Gly Ile Asn           
    210                 215                 220                           

acc gac ggc gcc cac aac ccg gtc aca aac cgc atc gtg cag tcg ggc        720
Thr Asp Gly Ala His Asn Pro Val Thr Asn Arg Ile Val Gln Ser Gly           
225                 230                 235                 240           

gat atc ctt tcg ctc aat acc ttc ccg atg atc ttc ggc tat tac acc        768
Asp Ile Leu Ser Leu Asn Thr Phe Pro Met Ile Phe Gly Tyr Tyr Thr           
                245                 250                 255               

gcg ctc gag cgg acg ctg ttc tgc gac cat gtc gac gat gcc agc ctc        816
Ala Leu Glu Arg Thr Leu Phe Cys Asp His Val Asp Asp Ala Ser Leu           
            260                 265                 270                   

gat atc tgg gag aag aac gtg gcc gtc cac cgt cgc ggg ctg gag ctg        864
Asp Ile Trp Glu Lys Asn Val Ala Val His Arg Arg Gly Leu Glu Leu           
        275                 280                 285                       

atc aag ccg ggc gcc cgc tgc aag gac atc gcg ctc gaa ctc aac gac        912
Ile Lys Pro Gly Ala Arg Cys Lys Asp Ile Ala Leu Glu Leu Asn Asp           
    290                 295                 300                           

atg tat cgc gag tgg gac ctg ctg aag tac cgt tcg ttc ggc tac ggc        960
Met Tyr Arg Glu Trp Asp Leu Leu Lys Tyr Arg Ser Phe Gly Tyr Gly           
305                 310                 315                 320           

cat tcc ttc ggc gtg ctg tgc cac tac tat ggc cgc gag gcc ggg gtg       1008
His Ser Phe Gly Val Leu Cys His Tyr Tyr Gly Arg Glu Ala Gly Val           
                325                 330                 335               

gaa ctg cgc gag gac atc gac acg gtg ctg gag ccc ggc atg gtg gtt       1056
Glu Leu Arg Glu Asp Ile Asp Thr Val Leu Glu Pro Gly Met Val Val           
            340                 345                 350                   

tcc atg gag ccg atg gtg atg ctg ccg gaa ggc gcg ccg ggc gcc ggc       1104
Ser Met Glu Pro Met Val Met Leu Pro Glu Gly Ala Pro Gly Ala Gly           
        355                 360                 365                       

ggc tat cgc gag cac gac atc ctg atc gtc aag gag gac agc gcc gag       1152
Gly Tyr Arg Glu His Asp Ile Leu Ile Val Lys Glu Asp Ser Ala Glu           
    370                 375                 380                           

aac atc acc ggc ttc ccc ttc ggc ccc gag cac aac atc atc aag aac       1200
Asn Ile Thr Gly Phe Pro Phe Gly Pro Glu His Asn Ile Ile Lys Asn           
385                 390                 395                 400           

tga                                                                   1203


<210>  6
<211>  400
<212>  PRT
<213>  Ochrobactrum anthropi

<400>  6

Met Leu His Val Met Lys Trp His Asn Gly Glu Lys Asp Tyr Ser Pro 
1               5                   10                  15      


Phe Ser Glu Ala Glu Met Thr Arg Arg Gln Asn Asp Val Arg Gly Trp 
            20                  25                  30          


Met Ala Lys Asn Asp Val Asp Ala Ala Leu Phe Thr Ser Tyr His Cys 
        35                  40                  45              


Ile Asn Tyr Tyr Ser Gly Trp Leu Tyr Cys Tyr Phe Gly Arg Lys Tyr 
    50                  55                  60                  


Gly Met Val Ile Asp His Asn Lys Ala Thr Thr Ile Ser Ala Gly Ile 
65                  70                  75                  80  


Asp Gly Gly Gln Pro Trp Arg Arg Ser Phe Gly Asp Asn Ile Thr Tyr 
                85                  90                  95      


Thr Asp Trp Arg Arg Asp Asn Phe Tyr Gln Ala Val Arg Gln Leu Thr 
            100                 105                 110         


Lys Gly Ala Lys Arg Val Gly Ile Glu Phe Asp His Val Ser Leu Asp 
        115                 120                 125             


Phe Arg Arg Gln Leu Glu Glu Ala Leu Pro Gly Val Glu Phe Val Asp 
    130                 135                 140                 


Val Gly Gln Pro Ser Met Trp Met Arg Thr Ile Lys Ser Ala Glu Glu 
145                 150                 155                 160 


Gln Lys Leu Ile Arg Glu Gly Ala Arg Val Cys Asp Val Gly Gly Ala 
                165                 170                 175     


Ala Cys Ala Ala Ala Val Lys Ala Gly Val Pro Glu His Glu Val Ala 
            180                 185                 190         


Ile Ala Thr Thr Asn Ala Met Val Arg Glu Ile Ala Lys Ser Phe Pro 
        195                 200                 205             


Phe Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln Ser Gly Ile Asn 
    210                 215                 220                 


Thr Asp Gly Ala His Asn Pro Val Thr Asn Arg Ile Val Gln Ser Gly 
225                 230                 235                 240 


Asp Ile Leu Ser Leu Asn Thr Phe Pro Met Ile Phe Gly Tyr Tyr Thr 
                245                 250                 255     


Ala Leu Glu Arg Thr Leu Phe Cys Asp His Val Asp Asp Ala Ser Leu 
            260                 265                 270         


Asp Ile Trp Glu Lys Asn Val Ala Val His Arg Arg Gly Leu Glu Leu 
        275                 280                 285             


Ile Lys Pro Gly Ala Arg Cys Lys Asp Ile Ala Leu Glu Leu Asn Asp 
    290                 295                 300                 


Met Tyr Arg Glu Trp Asp Leu Leu Lys Tyr Arg Ser Phe Gly Tyr Gly 
305                 310                 315                 320 


His Ser Phe Gly Val Leu Cys His Tyr Tyr Gly Arg Glu Ala Gly Val 
                325                 330                 335     


Glu Leu Arg Glu Asp Ile Asp Thr Val Leu Glu Pro Gly Met Val Val 
            340                 345                 350         


Ser Met Glu Pro Met Val Met Leu Pro Glu Gly Ala Pro Gly Ala Gly 
        355                 360                 365             


Gly Tyr Arg Glu His Asp Ile Leu Ile Val Lys Glu Asp Ser Ala Glu 
    370                 375                 380                 


Asn Ile Thr Gly Phe Pro Phe Gly Pro Glu His Asn Ile Ile Lys Asn 
385                 390                 395                 400 


<210>  7
<211>  400
<212>  PRT
<213>  Ochrobactrum anthropi


<220>
<221>  mat_peptide
<222>  (1)..(400)
<223>  Mutant creatine amidinohydrolase _Ochrobactrum anthropi 
       A0A076WGB5 with C171A+C295A substitutions

<400>  7

Met Leu His Val Met Lys Trp His Asn Gly Glu Lys Asp Tyr Ser Pro 
1               5                   10                  15      


Phe Ser Glu Ala Glu Met Thr Arg Arg Gln Asn Asp Val Arg Gly Trp 
            20                  25                  30          


Met Ala Lys Asn Asp Val Asp Ala Ala Leu Phe Thr Ser Tyr His Cys 
        35                  40                  45              


Ile Asn Tyr Tyr Ser Gly Trp Leu Tyr Cys Tyr Phe Gly Arg Lys Tyr 
    50                  55                  60                  


Gly Met Val Ile Asp His Asn Lys Ala Thr Thr Ile Ser Ala Gly Ile 
65                  70                  75                  80  


Asp Gly Gly Gln Pro Trp Arg Arg Ser Phe Gly Asp Asn Ile Thr Tyr 
                85                  90                  95      


Thr Asp Trp Arg Arg Asp Asn Phe Tyr Gln Ala Val Arg Gln Leu Thr 
            100                 105                 110         


Lys Gly Ala Lys Arg Val Gly Ile Glu Phe Asp His Val Ser Leu Asp 
        115                 120                 125             


Phe Arg Arg Gln Leu Glu Glu Ala Leu Pro Gly Val Glu Phe Val Asp 
    130                 135                 140                 


Val Gly Gln Pro Ser Met Trp Met Arg Thr Ile Lys Ser Ala Glu Glu 
145                 150                 155                 160 


Gln Lys Leu Ile Arg Glu Gly Ala Arg Val Ala Asp Val Gly Gly Ala 
                165                 170                 175     


Ala Cys Ala Ala Ala Val Lys Ala Gly Val Pro Glu His Glu Val Ala 
            180                 185                 190         


Ile Ala Thr Thr Asn Ala Met Val Arg Glu Ile Ala Lys Ser Phe Pro 
        195                 200                 205             


Phe Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln Ser Gly Ile Asn 
    210                 215                 220                 


Thr Asp Gly Ala His Asn Pro Val Thr Asn Arg Ile Val Gln Ser Gly 
225                 230                 235                 240 


Asp Ile Leu Ser Leu Asn Thr Phe Pro Met Ile Phe Gly Tyr Tyr Thr 
                245                 250                 255     


Ala Leu Glu Arg Thr Leu Phe Cys Asp His Val Asp Asp Ala Ser Leu 
            260                 265                 270         


Asp Ile Trp Glu Lys Asn Val Ala Val His Arg Arg Gly Leu Glu Leu 
        275                 280                 285             


Ile Lys Pro Gly Ala Arg Ala Lys Asp Ile Ala Leu Glu Leu Asn Asp 
    290                 295                 300                 


Met Tyr Arg Glu Trp Asp Leu Leu Lys Tyr Arg Ser Phe Gly Tyr Gly 
305                 310                 315                 320 


His Ser Phe Gly Val Leu Cys His Tyr Tyr Gly Arg Glu Ala Gly Val 
                325                 330                 335     


Glu Leu Arg Glu Asp Ile Asp Thr Val Leu Glu Pro Gly Met Val Val 
            340                 345                 350         


Ser Met Glu Pro Met Val Met Leu Pro Glu Gly Ala Pro Gly Ala Gly 
        355                 360                 365             


Gly Tyr Arg Glu His Asp Ile Leu Ile Val Lys Glu Asp Ser Ala Glu 
    370                 375                 380                 


Asn Ile Thr Gly Phe Pro Phe Gly Pro Glu His Asn Ile Ile Lys Asn 
385                 390                 395                 400 


<210>  8
<211>  400
<212>  PRT
<213>  Ochrobactrum anthropi


<220>
<221>  mat_peptide
<222>  (1)..(400)
<223>  Mutant creatine amidinohydrolase _Ochrobactrum anthropi 
       A0A076WGB5 with C171A+C295A+C264L substitutions

<400>  8

Met Leu His Val Met Lys Trp His Asn Gly Glu Lys Asp Tyr Ser Pro 
1               5                   10                  15      


Phe Ser Glu Ala Glu Met Thr Arg Arg Gln Asn Asp Val Arg Gly Trp 
            20                  25                  30          


Met Ala Lys Asn Asp Val Asp Ala Ala Leu Phe Thr Ser Tyr His Cys 
        35                  40                  45              


Ile Asn Tyr Tyr Ser Gly Trp Leu Tyr Cys Tyr Phe Gly Arg Lys Tyr 
    50                  55                  60                  


Gly Met Val Ile Asp His Asn Lys Ala Thr Thr Ile Ser Ala Gly Ile 
65                  70                  75                  80  


Asp Gly Gly Gln Pro Trp Arg Arg Ser Phe Gly Asp Asn Ile Thr Tyr 
                85                  90                  95      


Thr Asp Trp Arg Arg Asp Asn Phe Tyr Gln Ala Val Arg Gln Leu Thr 
            100                 105                 110         


Lys Gly Ala Lys Arg Val Gly Ile Glu Phe Asp His Val Ser Leu Asp 
        115                 120                 125             


Phe Arg Arg Gln Leu Glu Glu Ala Leu Pro Gly Val Glu Phe Val Asp 
    130                 135                 140                 


Val Gly Gln Pro Ser Met Trp Met Arg Thr Ile Lys Ser Ala Glu Glu 
145                 150                 155                 160 


Gln Lys Leu Ile Arg Glu Gly Ala Arg Val Ala Asp Val Gly Gly Ala 
                165                 170                 175     


Ala Cys Ala Ala Ala Val Lys Ala Gly Val Pro Glu His Glu Val Ala 
            180                 185                 190         


Ile Ala Thr Thr Asn Ala Met Val Arg Glu Ile Ala Lys Ser Phe Pro 
        195                 200                 205             


Phe Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln Ser Gly Ile Asn 
    210                 215                 220                 


Thr Asp Gly Ala His Asn Pro Val Thr Asn Arg Ile Val Gln Ser Gly 
225                 230                 235                 240 


Asp Ile Leu Ser Leu Asn Thr Phe Pro Met Ile Phe Gly Tyr Tyr Thr 
                245                 250                 255     


Ala Leu Glu Arg Thr Leu Phe Leu Asp His Val Asp Asp Ala Ser Leu 
            260                 265                 270         


Asp Ile Trp Glu Lys Asn Val Ala Val His Arg Arg Gly Leu Glu Leu 
        275                 280                 285             


Ile Lys Pro Gly Ala Arg Ala Lys Asp Ile Ala Leu Glu Leu Asn Asp 
    290                 295                 300                 


Met Tyr Arg Glu Trp Asp Leu Leu Lys Tyr Arg Ser Phe Gly Tyr Gly 
305                 310                 315                 320 


His Ser Phe Gly Val Leu Cys His Tyr Tyr Gly Arg Glu Ala Gly Val 
                325                 330                 335     


Glu Leu Arg Glu Asp Ile Asp Thr Val Leu Glu Pro Gly Met Val Val 
            340                 345                 350         


Ser Met Glu Pro Met Val Met Leu Pro Glu Gly Ala Pro Gly Ala Gly 
        355                 360                 365             


Gly Tyr Arg Glu His Asp Ile Leu Ile Val Lys Glu Asp Ser Ala Glu 
    370                 375                 380                 


Asn Ile Thr Gly Phe Pro Phe Gly Pro Glu His Asn Ile Ile Lys Asn 
385                 390                 395                 400 


<210>  9
<211>  1215
<212>  DNA
<213>  Mesorhizobium sp


<220>
<221>  CDS
<222>  (1)..(1215)
<223>  ESY78205.1 Mesorhizobium sp. LNHC221B00 gene encoding creatine 
       amidinohydrolase [LNHC221B00]

<400>  9
atg act gac gac atg ctg cac gtg gtg aaa tgg cac aac gga gag aag         48
Met Thr Asp Asp Met Leu His Val Val Lys Trp His Asn Gly Glu Lys           
1               5                   10                  15                

gac tat tct ccc ttc tcg gaa gct gag atg aag cgc cgc caa aac gac         96
Asp Tyr Ser Pro Phe Ser Glu Ala Glu Met Lys Arg Arg Gln Asn Asp           
            20                  25                  30                    

gta cgt cgc tgg atg gcc gac aac aac gtc gac gcg gcc ctg ttc acc        144
Val Arg Arg Trp Met Ala Asp Asn Asn Val Asp Ala Ala Leu Phe Thr           
        35                  40                  45                        

tcc tat cat tgc atc aac tac tat tcc ggt tgg ctc tac tgc tac ttc        192
Ser Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp Leu Tyr Cys Tyr Phe           
    50                  55                  60                            

ggc cgc aag tac ggc atg gtc atc gac cag gac aac gcc acg acc atc        240
Gly Arg Lys Tyr Gly Met Val Ile Asp Gln Asp Asn Ala Thr Thr Ile           
65                  70                  75                  80            

tcg gcc ggc att gat ggc ggc cag ccc tat cgc cgc agc ttc ggc gac        288
Ser Ala Gly Ile Asp Gly Gly Gln Pro Tyr Arg Arg Ser Phe Gly Asp           
                85                  90                  95                

aac att acc tac acg gac tgg cgc cgc gac aac tac tat cgc gcc gtg        336
Asn Ile Thr Tyr Thr Asp Trp Arg Arg Asp Asn Tyr Tyr Arg Ala Val           
            100                 105                 110                   

cgc cag ttg acg gcg ggc gcc aag cgc gtc ggc atc gag ttc gat cat        384
Arg Gln Leu Thr Ala Gly Ala Lys Arg Val Gly Ile Glu Phe Asp His           
        115                 120                 125                       

gtc aat ctc gat ttc cgc cgg caa ctc gaa gag gcg ctg ccg ggc gtc        432
Val Asn Leu Asp Phe Arg Arg Gln Leu Glu Glu Ala Leu Pro Gly Val           
    130                 135                 140                           

gaa ttc atc gat att gcc caa ccc tcg atg tgg atg cgc tcg atc aaa        480
Glu Phe Ile Asp Ile Ala Gln Pro Ser Met Trp Met Arg Ser Ile Lys           
145                 150                 155                 160           

tcg gtg gag gag cac acc ttg atc cgc gag ggg gcg cgc gtc agc gac        528
Ser Val Glu Glu His Thr Leu Ile Arg Glu Gly Ala Arg Val Ser Asp           
                165                 170                 175               

gtc ggc ggc gca gcg tgc gtg gcg gcg gta aag gcc ggc gtt ccg gag        576
Val Gly Gly Ala Ala Cys Val Ala Ala Val Lys Ala Gly Val Pro Glu           
            180                 185                 190                   

cac gag gtg gcg atc gcc acc acc gat gca atg atc cgc gaa atc gcg        624
His Glu Val Ala Ile Ala Thr Thr Asp Ala Met Ile Arg Glu Ile Ala           
        195                 200                 205                       

aaa tcg cac ccc ttc gtc gaa ctg atg gac acc tgg acc tgg ttc cag        672
Lys Ser His Pro Phe Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln           
    210                 215                 220                           

tcg ggc atc aac acc gat ggc gcg cac aat ccg gtc acg aac cgc gtc        720
Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro Val Thr Asn Arg Val           
225                 230                 235                 240           

gtg cgg gct gga gac atc ctg tcg ctc aac acc ttt ccg atg atc ttc        768
Val Arg Ala Gly Asp Ile Leu Ser Leu Asn Thr Phe Pro Met Ile Phe           
                245                 250                 255               

ggc tac tac acc gca ctc gaa cgg acg ctg ttc tgc gac cac gcc gac        816
Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Leu Phe Cys Asp His Ala Asp           
            260                 265                 270                   

gat gcc agc ctg gac gta tgg cag aag aat gtc gcc gtg cat cgc cgt        864
Asp Ala Ser Leu Asp Val Trp Gln Lys Asn Val Ala Val His Arg Arg           
        275                 280                 285                       

ggt ctc gag ctg atc aag cct ggc gtc cgt tgc aag gac atc gca atc        912
Gly Leu Glu Leu Ile Lys Pro Gly Val Arg Cys Lys Asp Ile Ala Ile           
    290                 295                 300                           

gag ctc aac gag atg tac cgc gag tgg gat ctc ctg aaa tac cgg tcc        960
Glu Leu Asn Glu Met Tyr Arg Glu Trp Asp Leu Leu Lys Tyr Arg Ser           
305                 310                 315                 320           

ttc ggg tat ggc cac tcc ttc ggt gtg ctc tgc cac tat tat ggc cgc       1008
Phe Gly Tyr Gly His Ser Phe Gly Val Leu Cys His Tyr Tyr Gly Arg           
                325                 330                 335               

gag gct ggc gtc gag ttg cgt gag gac atc gaa aca gtg ctg gag ccc       1056
Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Glu Thr Val Leu Glu Pro           
            340                 345                 350                   

ggc atg gtg gtg tcg atg gag ccg atg gtc atg ttg ccg gaa ggc acg       1104
Gly Met Val Val Ser Met Glu Pro Met Val Met Leu Pro Glu Gly Thr           
        355                 360                 365                       

cca ggc gcc ggc ggc tac cgc gag cat gac atc ctg atc gtc aag gac       1152
Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile Leu Ile Val Lys Asp           
    370                 375                 380                           

gac ggc gcc gaa aac atc acc ggt ttc ccc ttc ggg ccg gaa cac aac       1200
Asp Gly Ala Glu Asn Ile Thr Gly Phe Pro Phe Gly Pro Glu His Asn           
385                 390                 395                 400           

atc atc agg aat tga                                                   1215
Ile Ile Arg Asn                                                           
                                                                          


<210>  10
<211>  404
<212>  PRT
<213>  Mesorhizobium sp

<400>  10

Met Thr Asp Asp Met Leu His Val Val Lys Trp His Asn Gly Glu Lys 
1               5                   10                  15      


Asp Tyr Ser Pro Phe Ser Glu Ala Glu Met Lys Arg Arg Gln Asn Asp 
            20                  25                  30          


Val Arg Arg Trp Met Ala Asp Asn Asn Val Asp Ala Ala Leu Phe Thr 
        35                  40                  45              


Ser Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp Leu Tyr Cys Tyr Phe 
    50                  55                  60                  


Gly Arg Lys Tyr Gly Met Val Ile Asp Gln Asp Asn Ala Thr Thr Ile 
65                  70                  75                  80  


Ser Ala Gly Ile Asp Gly Gly Gln Pro Tyr Arg Arg Ser Phe Gly Asp 
                85                  90                  95      


Asn Ile Thr Tyr Thr Asp Trp Arg Arg Asp Asn Tyr Tyr Arg Ala Val 
            100                 105                 110         


Arg Gln Leu Thr Ala Gly Ala Lys Arg Val Gly Ile Glu Phe Asp His 
        115                 120                 125             


Val Asn Leu Asp Phe Arg Arg Gln Leu Glu Glu Ala Leu Pro Gly Val 
    130                 135                 140                 


Glu Phe Ile Asp Ile Ala Gln Pro Ser Met Trp Met Arg Ser Ile Lys 
145                 150                 155                 160 


Ser Val Glu Glu His Thr Leu Ile Arg Glu Gly Ala Arg Val Ser Asp 
                165                 170                 175     


Val Gly Gly Ala Ala Cys Val Ala Ala Val Lys Ala Gly Val Pro Glu 
            180                 185                 190         


His Glu Val Ala Ile Ala Thr Thr Asp Ala Met Ile Arg Glu Ile Ala 
        195                 200                 205             


Lys Ser His Pro Phe Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln 
    210                 215                 220                 


Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro Val Thr Asn Arg Val 
225                 230                 235                 240 


Val Arg Ala Gly Asp Ile Leu Ser Leu Asn Thr Phe Pro Met Ile Phe 
                245                 250                 255     


Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Leu Phe Cys Asp His Ala Asp 
            260                 265                 270         


Asp Ala Ser Leu Asp Val Trp Gln Lys Asn Val Ala Val His Arg Arg 
        275                 280                 285             


Gly Leu Glu Leu Ile Lys Pro Gly Val Arg Cys Lys Asp Ile Ala Ile 
    290                 295                 300                 


Glu Leu Asn Glu Met Tyr Arg Glu Trp Asp Leu Leu Lys Tyr Arg Ser 
305                 310                 315                 320 


Phe Gly Tyr Gly His Ser Phe Gly Val Leu Cys His Tyr Tyr Gly Arg 
                325                 330                 335     


Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Glu Thr Val Leu Glu Pro 
            340                 345                 350         


Gly Met Val Val Ser Met Glu Pro Met Val Met Leu Pro Glu Gly Thr 
        355                 360                 365             


Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile Leu Ile Val Lys Asp 
    370                 375                 380                 


Asp Gly Ala Glu Asn Ile Thr Gly Phe Pro Phe Gly Pro Glu His Asn 
385                 390                 395                 400 


Ile Ile Arg Asn 
                


<210>  11
<211>  404
<212>  PRT
<213>  Mesorhizobium sp


<220>
<221>  mat_peptide
<222>  (1)..(404)
<223>  Mutant creatine amidinohydrolase _Mesorhizobium sp.  X6DLM3 with 
       S175A+C299A substitutions

<400>  11

Met Thr Asp Asp Met Leu His Val Val Lys Trp His Asn Gly Glu Lys 
1               5                   10                  15      


Asp Tyr Ser Pro Phe Ser Glu Ala Glu Met Lys Arg Arg Gln Asn Asp 
            20                  25                  30          


Val Arg Arg Trp Met Ala Asp Asn Asn Val Asp Ala Ala Leu Phe Thr 
        35                  40                  45              


Ser Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp Leu Tyr Cys Tyr Phe 
    50                  55                  60                  


Gly Arg Lys Tyr Gly Met Val Ile Asp Gln Asp Asn Ala Thr Thr Ile 
65                  70                  75                  80  


Ser Ala Gly Ile Asp Gly Gly Gln Pro Tyr Arg Arg Ser Phe Gly Asp 
                85                  90                  95      


Asn Ile Thr Tyr Thr Asp Trp Arg Arg Asp Asn Tyr Tyr Arg Ala Val 
            100                 105                 110         


Arg Gln Leu Thr Ala Gly Ala Lys Arg Val Gly Ile Glu Phe Asp His 
        115                 120                 125             


Val Asn Leu Asp Phe Arg Arg Gln Leu Glu Glu Ala Leu Pro Gly Val 
    130                 135                 140                 


Glu Phe Ile Asp Ile Ala Gln Pro Ser Met Trp Met Arg Ser Ile Lys 
145                 150                 155                 160 


Ser Val Glu Glu His Thr Leu Ile Arg Glu Gly Ala Arg Val Ala Asp 
                165                 170                 175     


Val Gly Gly Ala Ala Cys Val Ala Ala Val Lys Ala Gly Val Pro Glu 
            180                 185                 190         


His Glu Val Ala Ile Ala Thr Thr Asp Ala Met Ile Arg Glu Ile Ala 
        195                 200                 205             


Lys Ser His Pro Phe Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln 
    210                 215                 220                 


Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro Val Thr Asn Arg Val 
225                 230                 235                 240 


Val Arg Ala Gly Asp Ile Leu Ser Leu Asn Thr Phe Pro Met Ile Phe 
                245                 250                 255     


Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Leu Phe Cys Asp His Ala Asp 
            260                 265                 270         


Asp Ala Ser Leu Asp Val Trp Gln Lys Asn Val Ala Val His Arg Arg 
        275                 280                 285             


Gly Leu Glu Leu Ile Lys Pro Gly Val Arg Ala Lys Asp Ile Ala Ile 
    290                 295                 300                 


Glu Leu Asn Glu Met Tyr Arg Glu Trp Asp Leu Leu Lys Tyr Arg Ser 
305                 310                 315                 320 


Phe Gly Tyr Gly His Ser Phe Gly Val Leu Cys His Tyr Tyr Gly Arg 
                325                 330                 335     


Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Glu Thr Val Leu Glu Pro 
            340                 345                 350         


Gly Met Val Val Ser Met Glu Pro Met Val Met Leu Pro Glu Gly Thr 
        355                 360                 365             


Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile Leu Ile Val Lys Asp 
    370                 375                 380                 


Asp Gly Ala Glu Asn Ile Thr Gly Phe Pro Phe Gly Pro Glu His Asn 
385                 390                 395                 400 


Ile Ile Arg Asn 
                


<210>  12
<211>  404
<212>  PRT
<213>  Mesorhizobium sp


<220>
<221>  mat_peptide
<222>  (1)..(404)
<223>  Mutant creatine amidinohydrolase _Mesorhizobium sp.  X6DLM3 with 
       S175A+C299A+C268L substitutions

<400>  12

Met Thr Asp Asp Met Leu His Val Val Lys Trp His Asn Gly Glu Lys 
1               5                   10                  15      


Asp Tyr Ser Pro Phe Ser Glu Ala Glu Met Lys Arg Arg Gln Asn Asp 
            20                  25                  30          


Val Arg Arg Trp Met Ala Asp Asn Asn Val Asp Ala Ala Leu Phe Thr 
        35                  40                  45              


Ser Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp Leu Tyr Cys Tyr Phe 
    50                  55                  60                  


Gly Arg Lys Tyr Gly Met Val Ile Asp Gln Asp Asn Ala Thr Thr Ile 
65                  70                  75                  80  


Ser Ala Gly Ile Asp Gly Gly Gln Pro Tyr Arg Arg Ser Phe Gly Asp 
                85                  90                  95      


Asn Ile Thr Tyr Thr Asp Trp Arg Arg Asp Asn Tyr Tyr Arg Ala Val 
            100                 105                 110         


Arg Gln Leu Thr Ala Gly Ala Lys Arg Val Gly Ile Glu Phe Asp His 
        115                 120                 125             


Val Asn Leu Asp Phe Arg Arg Gln Leu Glu Glu Ala Leu Pro Gly Val 
    130                 135                 140                 


Glu Phe Ile Asp Ile Ala Gln Pro Ser Met Trp Met Arg Ser Ile Lys 
145                 150                 155                 160 


Ser Val Glu Glu His Thr Leu Ile Arg Glu Gly Ala Arg Val Ala Asp 
                165                 170                 175     


Val Gly Gly Ala Ala Cys Val Ala Ala Val Lys Ala Gly Val Pro Glu 
            180                 185                 190         


His Glu Val Ala Ile Ala Thr Thr Asp Ala Met Ile Arg Glu Ile Ala 
        195                 200                 205             


Lys Ser His Pro Phe Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln 
    210                 215                 220                 


Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro Val Thr Asn Arg Val 
225                 230                 235                 240 


Val Arg Ala Gly Asp Ile Leu Ser Leu Asn Thr Phe Pro Met Ile Phe 
                245                 250                 255     


Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Leu Phe Leu Asp His Ala Asp 
            260                 265                 270         


Asp Ala Ser Leu Asp Val Trp Gln Lys Asn Val Ala Val His Arg Arg 
        275                 280                 285             


Gly Leu Glu Leu Ile Lys Pro Gly Val Arg Ala Lys Asp Ile Ala Ile 
    290                 295                 300                 


Glu Leu Asn Glu Met Tyr Arg Glu Trp Asp Leu Leu Lys Tyr Arg Ser 
305                 310                 315                 320 


Phe Gly Tyr Gly His Ser Phe Gly Val Leu Cys His Tyr Tyr Gly Arg 
                325                 330                 335     


Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Glu Thr Val Leu Glu Pro 
            340                 345                 350         


Gly Met Val Val Ser Met Glu Pro Met Val Met Leu Pro Glu Gly Thr 
        355                 360                 365             


Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile Leu Ile Val Lys Asp 
    370                 375                 380                 


Asp Gly Ala Glu Asn Ile Thr Gly Phe Pro Phe Gly Pro Glu His Asn 
385                 390                 395                 400 


Ile Ile Arg Asn 
                


<210>  13
<211>  1218
<212>  DNA
<213>  Roseovarius sp


<220>
<221>  CDS
<222>  (1)..(1218)
<223>  EDM33382.1 Roseovarius sp. TM1035 gene encoding creatine 
       amidinohydrolase [A6DVF8]

<400>  13
atg ctt gac gac atg ttg cac gtc acg gaa tgg cac aac ggc gaa aag         48
Met Leu Asp Asp Met Leu His Val Thr Glu Trp His Asn Gly Glu Lys           
1               5                   10                  15                

gag ttt tcg ccc ttt tcc gac aat gaa atg gcc cgc cgc cag aac gaa         96
Glu Phe Ser Pro Phe Ser Asp Asn Glu Met Ala Arg Arg Gln Asn Glu           
            20                  25                  30                    

ttg cgc gtc tgg atg gcc gat aac aac gtc gat gcg gcg ctc ttc acg        144
Leu Arg Val Trp Met Ala Asp Asn Asn Val Asp Ala Ala Leu Phe Thr           
        35                  40                  45                        

tcc tat cac tgc att aac tat tac tca ggc tgg ctc tac tgc tat ttc        192
Ser Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp Leu Tyr Cys Tyr Phe           
    50                  55                  60                            

ggt cgc aaa tat ggc atg gtc att gac caa aag aac gcc acg acc att        240
Gly Arg Lys Tyr Gly Met Val Ile Asp Gln Lys Asn Ala Thr Thr Ile           
65                  70                  75                  80            

tcc gca ggc atc gac ggc ggc cag ccc tgg cgg cga aca ttc ggc agc        288
Ser Ala Gly Ile Asp Gly Gly Gln Pro Trp Arg Arg Thr Phe Gly Ser           
                85                  90                  95                

aat gtc acc tat acc gac tgg cgg cgc gac aat ttc tac cgc gcg gtg        336
Asn Val Thr Tyr Thr Asp Trp Arg Arg Asp Asn Phe Tyr Arg Ala Val           
            100                 105                 110                   

cag ggc ctg acc aag ggt gcc cgc cgc gtc ggc atc gag ttt gac cat        384
Gln Gly Leu Thr Lys Gly Ala Arg Arg Val Gly Ile Glu Phe Asp His           
        115                 120                 125                       

gtt tcg ctc gat tat cgc cag ctt ttg cag gat gcc ctg ccc ggc gtc        432
Val Ser Leu Asp Tyr Arg Gln Leu Leu Gln Asp Ala Leu Pro Gly Val           
    130                 135                 140                           

gaa ttg gtg gac gtg agc caa ccc tcg atg tgg atg cgc acc atc aaa        480
Glu Leu Val Asp Val Ser Gln Pro Ser Met Trp Met Arg Thr Ile Lys           
145                 150                 155                 160           

tcc gcc gag gaa atc aag ctc atc acc gaa ggc gcg cgc atc tgt gac        528
Ser Ala Glu Glu Ile Lys Leu Ile Thr Glu Gly Ala Arg Ile Cys Asp           
                165                 170                 175               

gtg ggg ggc tat gcc gtg gcg ggc gct gtc aag gca ggc gtg ccc gaa        576
Val Gly Gly Tyr Ala Val Ala Gly Ala Val Lys Ala Gly Val Pro Glu           
            180                 185                 190                   

cac gaa gtg gcg att gcg ggc aca aat gcg atg atc cgc gag att gcc        624
His Glu Val Ala Ile Ala Gly Thr Asn Ala Met Ile Arg Glu Ile Ala           
        195                 200                 205                       

aaa tcc ttc ccc ttt gtc gaa ctg atg gac acc tgg aca tgg ttc cag        672
Lys Ser Phe Pro Phe Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln           
    210                 215                 220                           

tcg ggc atc aac acc gat ggc gcg cat aac ccc gtg acc aac cgc gtg        720
Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro Val Thr Asn Arg Val           
225                 230                 235                 240           

gtg caa tcg ggc gat atc ctc agc ctc aac acc ttc ccg atg atc ttt        768
Val Gln Ser Gly Asp Ile Leu Ser Leu Asn Thr Phe Pro Met Ile Phe           
                245                 250                 255               

ggc tat tac acc gcg ctc gaa cgc acg ctt ttt tgc gat cat gtc gat        816
Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Leu Phe Cys Asp His Val Asp           
            260                 265                 270                   

gac gcc agc ctc gac atc tgg gag aaa aac gtc gcc gtg cat cgt cgc        864
Asp Ala Ser Leu Asp Ile Trp Glu Lys Asn Val Ala Val His Arg Arg           
        275                 280                 285                       

ggg ctg gag ttg atg aaa ccc ggc gcg cgc tgc atg gat atc gcg att        912
Gly Leu Glu Leu Met Lys Pro Gly Ala Arg Cys Met Asp Ile Ala Ile           
    290                 295                 300                           

gag ctg aac gag atg tat cgc gaa tgg gac ctg ctg aaa tac cgc tct        960
Glu Leu Asn Glu Met Tyr Arg Glu Trp Asp Leu Leu Lys Tyr Arg Ser           
305                 310                 315                 320           

ttc gga tat ggc cac agc ttt ggc gtg ctc agc cac tat tat ggc cgt       1008
Phe Gly Tyr Gly His Ser Phe Gly Val Leu Ser His Tyr Tyr Gly Arg           
                325                 330                 335               

gag gcg ggc gtg gaa ctg cgc gag gat atc gac acc gtg ctg aaa ccc       1056
Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Asp Thr Val Leu Lys Pro           
            340                 345                 350                   

ggc atg gtc gtg tcg atg gaa ccc atg gtg atg atc ccc gaa ggc cag       1104
Gly Met Val Val Ser Met Glu Pro Met Val Met Ile Pro Glu Gly Gln           
        355                 360                 365                       

ccc ggc gcc ggt ggc tac cgc gag cat gac att ctg gtc atc gga gaa       1152
Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile Leu Val Ile Gly Glu           
    370                 375                 380                           

gac ggg gcc gag aat atc acc ggc ttc ccc ttc gga cct gaa cat aac       1200
Asp Gly Ala Glu Asn Ile Thr Gly Phe Pro Phe Gly Pro Glu His Asn           
385                 390                 395                 400           

atc gtc ggc aaa ggc taa                                               1218
Ile Val Gly Lys Gly                                                       
                405                                                       


<210>  14
<211>  405
<212>  PRT
<213>  Roseovarius sp

<400>  14

Met Leu Asp Asp Met Leu His Val Thr Glu Trp His Asn Gly Glu Lys 
1               5                   10                  15      


Glu Phe Ser Pro Phe Ser Asp Asn Glu Met Ala Arg Arg Gln Asn Glu 
            20                  25                  30          


Leu Arg Val Trp Met Ala Asp Asn Asn Val Asp Ala Ala Leu Phe Thr 
        35                  40                  45              


Ser Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp Leu Tyr Cys Tyr Phe 
    50                  55                  60                  


Gly Arg Lys Tyr Gly Met Val Ile Asp Gln Lys Asn Ala Thr Thr Ile 
65                  70                  75                  80  


Ser Ala Gly Ile Asp Gly Gly Gln Pro Trp Arg Arg Thr Phe Gly Ser 
                85                  90                  95      


Asn Val Thr Tyr Thr Asp Trp Arg Arg Asp Asn Phe Tyr Arg Ala Val 
            100                 105                 110         


Gln Gly Leu Thr Lys Gly Ala Arg Arg Val Gly Ile Glu Phe Asp His 
        115                 120                 125             


Val Ser Leu Asp Tyr Arg Gln Leu Leu Gln Asp Ala Leu Pro Gly Val 
    130                 135                 140                 


Glu Leu Val Asp Val Ser Gln Pro Ser Met Trp Met Arg Thr Ile Lys 
145                 150                 155                 160 


Ser Ala Glu Glu Ile Lys Leu Ile Thr Glu Gly Ala Arg Ile Cys Asp 
                165                 170                 175     


Val Gly Gly Tyr Ala Val Ala Gly Ala Val Lys Ala Gly Val Pro Glu 
            180                 185                 190         


His Glu Val Ala Ile Ala Gly Thr Asn Ala Met Ile Arg Glu Ile Ala 
        195                 200                 205             


Lys Ser Phe Pro Phe Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln 
    210                 215                 220                 


Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro Val Thr Asn Arg Val 
225                 230                 235                 240 


Val Gln Ser Gly Asp Ile Leu Ser Leu Asn Thr Phe Pro Met Ile Phe 
                245                 250                 255     


Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Leu Phe Cys Asp His Val Asp 
            260                 265                 270         


Asp Ala Ser Leu Asp Ile Trp Glu Lys Asn Val Ala Val His Arg Arg 
        275                 280                 285             


Gly Leu Glu Leu Met Lys Pro Gly Ala Arg Cys Met Asp Ile Ala Ile 
    290                 295                 300                 


Glu Leu Asn Glu Met Tyr Arg Glu Trp Asp Leu Leu Lys Tyr Arg Ser 
305                 310                 315                 320 


Phe Gly Tyr Gly His Ser Phe Gly Val Leu Ser His Tyr Tyr Gly Arg 
                325                 330                 335     


Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Asp Thr Val Leu Lys Pro 
            340                 345                 350         


Gly Met Val Val Ser Met Glu Pro Met Val Met Ile Pro Glu Gly Gln 
        355                 360                 365             


Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile Leu Val Ile Gly Glu 
    370                 375                 380                 


Asp Gly Ala Glu Asn Ile Thr Gly Phe Pro Phe Gly Pro Glu His Asn 
385                 390                 395                 400 


Ile Val Gly Lys Gly 
                405 


<210>  15
<211>  405
<212>  PRT
<213>  Roseovarius sp.


<220>
<221>  mat_peptide
<222>  (1)..(405)
<223>  Mutant creatine amidinohydrolase _Roseovarius sp. A6DVF8 with 
       C175A+C299A substitutions

<400>  15

Met Leu Asp Asp Met Leu His Val Thr Glu Trp His Asn Gly Glu Lys 
1               5                   10                  15      


Glu Phe Ser Pro Phe Ser Asp Asn Glu Met Ala Arg Arg Gln Asn Glu 
            20                  25                  30          


Leu Arg Val Trp Met Ala Asp Asn Asn Val Asp Ala Ala Leu Phe Thr 
        35                  40                  45              


Ser Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp Leu Tyr Cys Tyr Phe 
    50                  55                  60                  


Gly Arg Lys Tyr Gly Met Val Ile Asp Gln Lys Asn Ala Thr Thr Ile 
65                  70                  75                  80  


Ser Ala Gly Ile Asp Gly Gly Gln Pro Trp Arg Arg Thr Phe Gly Ser 
                85                  90                  95      


Asn Val Thr Tyr Thr Asp Trp Arg Arg Asp Asn Phe Tyr Arg Ala Val 
            100                 105                 110         


Gln Gly Leu Thr Lys Gly Ala Arg Arg Val Gly Ile Glu Phe Asp His 
        115                 120                 125             


Val Ser Leu Asp Tyr Arg Gln Leu Leu Gln Asp Ala Leu Pro Gly Val 
    130                 135                 140                 


Glu Leu Val Asp Val Ser Gln Pro Ser Met Trp Met Arg Thr Ile Lys 
145                 150                 155                 160 


Ser Ala Glu Glu Ile Lys Leu Ile Thr Glu Gly Ala Arg Ile Ala Asp 
                165                 170                 175     


Val Gly Gly Tyr Ala Val Ala Gly Ala Val Lys Ala Gly Val Pro Glu 
            180                 185                 190         


His Glu Val Ala Ile Ala Gly Thr Asn Ala Met Ile Arg Glu Ile Ala 
        195                 200                 205             


Lys Ser Phe Pro Phe Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln 
    210                 215                 220                 


Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro Val Thr Asn Arg Val 
225                 230                 235                 240 


Val Gln Ser Gly Asp Ile Leu Ser Leu Asn Thr Phe Pro Met Ile Phe 
                245                 250                 255     


Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Leu Phe Cys Asp His Val Asp 
            260                 265                 270         


Asp Ala Ser Leu Asp Ile Trp Glu Lys Asn Val Ala Val His Arg Arg 
        275                 280                 285             


Gly Leu Glu Leu Met Lys Pro Gly Ala Arg Ala Met Asp Ile Ala Ile 
    290                 295                 300                 


Glu Leu Asn Glu Met Tyr Arg Glu Trp Asp Leu Leu Lys Tyr Arg Ser 
305                 310                 315                 320 


Phe Gly Tyr Gly His Ser Phe Gly Val Leu Ser His Tyr Tyr Gly Arg 
                325                 330                 335     


Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Asp Thr Val Leu Lys Pro 
            340                 345                 350         


Gly Met Val Val Ser Met Glu Pro Met Val Met Ile Pro Glu Gly Gln 
        355                 360                 365             


Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile Leu Val Ile Gly Glu 
    370                 375                 380                 


Asp Gly Ala Glu Asn Ile Thr Gly Phe Pro Phe Gly Pro Glu His Asn 
385                 390                 395                 400 


Ile Val Gly Lys Gly 
                405 


<210>  16
<211>  405
<212>  PRT
<213>  Roseovarius sp.


<220>
<221>  mat_peptide
<222>  (1)..(405)
<223>  Mutant creatine amidinohydrolase _Roseovarius sp. A6DVF8 with 
       C175A+C299A+C268L substitutions

<400>  16

Met Leu Asp Asp Met Leu His Val Thr Glu Trp His Asn Gly Glu Lys 
1               5                   10                  15      


Glu Phe Ser Pro Phe Ser Asp Asn Glu Met Ala Arg Arg Gln Asn Glu 
            20                  25                  30          


Leu Arg Val Trp Met Ala Asp Asn Asn Val Asp Ala Ala Leu Phe Thr 
        35                  40                  45              


Ser Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp Leu Tyr Cys Tyr Phe 
    50                  55                  60                  


Gly Arg Lys Tyr Gly Met Val Ile Asp Gln Lys Asn Ala Thr Thr Ile 
65                  70                  75                  80  


Ser Ala Gly Ile Asp Gly Gly Gln Pro Trp Arg Arg Thr Phe Gly Ser 
                85                  90                  95      


Asn Val Thr Tyr Thr Asp Trp Arg Arg Asp Asn Phe Tyr Arg Ala Val 
            100                 105                 110         


Gln Gly Leu Thr Lys Gly Ala Arg Arg Val Gly Ile Glu Phe Asp His 
        115                 120                 125             


Val Ser Leu Asp Tyr Arg Gln Leu Leu Gln Asp Ala Leu Pro Gly Val 
    130                 135                 140                 


Glu Leu Val Asp Val Ser Gln Pro Ser Met Trp Met Arg Thr Ile Lys 
145                 150                 155                 160 


Ser Ala Glu Glu Ile Lys Leu Ile Thr Glu Gly Ala Arg Ile Ala Asp 
                165                 170                 175     


Val Gly Gly Tyr Ala Val Ala Gly Ala Val Lys Ala Gly Val Pro Glu 
            180                 185                 190         


His Glu Val Ala Ile Ala Gly Thr Asn Ala Met Ile Arg Glu Ile Ala 
        195                 200                 205             


Lys Ser Phe Pro Phe Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln 
    210                 215                 220                 


Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro Val Thr Asn Arg Val 
225                 230                 235                 240 


Val Gln Ser Gly Asp Ile Leu Ser Leu Asn Thr Phe Pro Met Ile Phe 
                245                 250                 255     


Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Leu Phe Leu Asp His Val Asp 
            260                 265                 270         


Asp Ala Ser Leu Asp Ile Trp Glu Lys Asn Val Ala Val His Arg Arg 
        275                 280                 285             


Gly Leu Glu Leu Met Lys Pro Gly Ala Arg Ala Met Asp Ile Ala Ile 
    290                 295                 300                 


Glu Leu Asn Glu Met Tyr Arg Glu Trp Asp Leu Leu Lys Tyr Arg Ser 
305                 310                 315                 320 


Phe Gly Tyr Gly His Ser Phe Gly Val Leu Ser His Tyr Tyr Gly Arg 
                325                 330                 335     


Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Asp Thr Val Leu Lys Pro 
            340                 345                 350         


Gly Met Val Val Ser Met Glu Pro Met Val Met Ile Pro Glu Gly Gln 
        355                 360                 365             


Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile Leu Val Ile Gly Glu 
    370                 375                 380                 


Asp Gly Ala Glu Asn Ile Thr Gly Phe Pro Phe Gly Pro Glu His Asn 
385                 390                 395                 400 


Ile Val Gly Lys Gly 
                405 


<210>  17
<211>  1218
<212>  DNA
<213>  Roseovarius sp.


<220>
<221>  CDS
<222>  (1)..(1218)
<223>  EAQ25420.1 Roseovarius sp. 217 gene encoding creatine 
       amidinohydrolase [A3W1E4]

<400>  17
atg ctt gac gac atg ctg cac gtg acc gaa tgg cac aac gga gaa aag         48
Met Leu Asp Asp Met Leu His Val Thr Glu Trp His Asn Gly Glu Lys           
1               5                   10                  15                

gaa ttt tcg ccc ttt tcc gac aat gaa atg gcc cgc cgc cag aac gaa         96
Glu Phe Ser Pro Phe Ser Asp Asn Glu Met Ala Arg Arg Gln Asn Glu           
            20                  25                  30                    

ttg cgc gtc tgg atg gcc gac aac aat gtc gat gcg gcg ctc ttc acg        144
Leu Arg Val Trp Met Ala Asp Asn Asn Val Asp Ala Ala Leu Phe Thr           
        35                  40                  45                        

tcc tat cac tgc att aac tat tac tcg ggc tgg ctc tac tgc tat ttc        192
Ser Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp Leu Tyr Cys Tyr Phe           
    50                  55                  60                            

ggt cgc aaa tat ggc atg gtc att gac caa aag aac gcc acg acg att        240
Gly Arg Lys Tyr Gly Met Val Ile Asp Gln Lys Asn Ala Thr Thr Ile           
65                  70                  75                  80            

tcc gca ggc atc gac ggc ggt cag ccg tgg cgc cgg acc ttt ggc agc        288
Ser Ala Gly Ile Asp Gly Gly Gln Pro Trp Arg Arg Thr Phe Gly Ser           
                85                  90                  95                

aac gtc acc tat acc gac tgg cgg cgc gac aat ttc tat cgc gcg gtg        336
Asn Val Thr Tyr Thr Asp Trp Arg Arg Asp Asn Phe Tyr Arg Ala Val           
            100                 105                 110                   

cag ggc ctg acc aag ggc gcc cgc cgt gtg ggc atc gag ttt gac cat        384
Gln Gly Leu Thr Lys Gly Ala Arg Arg Val Gly Ile Glu Phe Asp His           
        115                 120                 125                       

gtc tcg ctc gac tac cgc caa ctc ttg cag gat gcg cta ccg ggc gtc        432
Val Ser Leu Asp Tyr Arg Gln Leu Leu Gln Asp Ala Leu Pro Gly Val           
    130                 135                 140                           

gaa ctg gtg gac gtg agc cag ccc tcg atg tgg atg cgc acc atc aaa        480
Glu Leu Val Asp Val Ser Gln Pro Ser Met Trp Met Arg Thr Ile Lys           
145                 150                 155                 160           

tcc gcc gag gaa atc aag ctc atc acc gaa ggc gcg cgc atc tgc gac        528
Ser Ala Glu Glu Ile Lys Leu Ile Thr Glu Gly Ala Arg Ile Cys Asp           
                165                 170                 175               

gtc ggc ggc tat gcc gtg gcc ggt gcc gtc aag gca ggc gtg ccc gag        576
Val Gly Gly Tyr Ala Val Ala Gly Ala Val Lys Ala Gly Val Pro Glu           
            180                 185                 190                   

cat gag gtg gcc atc gcg ggc acc aac gcg atg atc cgc gag atc gcc        624
His Glu Val Ala Ile Ala Gly Thr Asn Ala Met Ile Arg Glu Ile Ala           
        195                 200                 205                       

aaa tcc ttc ccc ttt gtc gaa ctg atg gat acc tgg aca tgg ttc cag        672
Lys Ser Phe Pro Phe Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln           
    210                 215                 220                           

tcg ggc atc aac acc gac ggc gcg cat aac ccc gtg acc aac cgc gtg        720
Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro Val Thr Asn Arg Val           
225                 230                 235                 240           

gtg caa tcg ggc gac atc ctc agc ctc aac acc ttt ccg atg atc ttt        768
Val Gln Ser Gly Asp Ile Leu Ser Leu Asn Thr Phe Pro Met Ile Phe           
                245                 250                 255               

ggc tat tac acc gcg ctc gaa cgc acg ctt ttt tgc gat cac gtc gat        816
Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Leu Phe Cys Asp His Val Asp           
            260                 265                 270                   

gac gcc agc ctc gac atc tgg gag aaa aac gtc gcc gtg cat cgc cgc        864
Asp Ala Ser Leu Asp Ile Trp Glu Lys Asn Val Ala Val His Arg Arg           
        275                 280                 285                       

ggg ctg gaa ctg atg aag ccc ggc gcg cgc tgc atg gat atc gcg atc        912
Gly Leu Glu Leu Met Lys Pro Gly Ala Arg Cys Met Asp Ile Ala Ile           
    290                 295                 300                           

gaa ctc aat gag atg tat cgc gac tgg gat ctg ctg aaa tac cgc tcc        960
Glu Leu Asn Glu Met Tyr Arg Asp Trp Asp Leu Leu Lys Tyr Arg Ser           
305                 310                 315                 320           

ttt ggc tac ggc cac agc ttt ggc gtg ctc agc cac tac tat ggc cgc       1008
Phe Gly Tyr Gly His Ser Phe Gly Val Leu Ser His Tyr Tyr Gly Arg           
                325                 330                 335               

gag gca ggg gtg gaa ctg cgc gag gat atc gac acc gtg ctg aaa ccc       1056
Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Asp Thr Val Leu Lys Pro           
            340                 345                 350                   

ggc atg gtc gtg tcg atg gaa ccg atg gtg atg atc ccc gag ggc cag       1104
Gly Met Val Val Ser Met Glu Pro Met Val Met Ile Pro Glu Gly Gln           
        355                 360                 365                       

ccc ggt gcc ggt ggc tac cgc gag cat gac att ctg gtc atc ggt gaa       1152
Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile Leu Val Ile Gly Glu           
    370                 375                 380                           

gat ggg gcc gag aat atc acc ggc ttt ccg ttc ggc ccc gaa cat aac       1200
Asp Gly Ala Glu Asn Ile Thr Gly Phe Pro Phe Gly Pro Glu His Asn           
385                 390                 395                 400           

atc gtc ggc aaa ggc taa                                               1218
Ile Val Gly Lys Gly                                                       
                405                                                       


<210>  18
<211>  405
<212>  PRT
<213>  Roseovarius sp.

<400>  18

Met Leu Asp Asp Met Leu His Val Thr Glu Trp His Asn Gly Glu Lys 
1               5                   10                  15      


Glu Phe Ser Pro Phe Ser Asp Asn Glu Met Ala Arg Arg Gln Asn Glu 
            20                  25                  30          


Leu Arg Val Trp Met Ala Asp Asn Asn Val Asp Ala Ala Leu Phe Thr 
        35                  40                  45              


Ser Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp Leu Tyr Cys Tyr Phe 
    50                  55                  60                  


Gly Arg Lys Tyr Gly Met Val Ile Asp Gln Lys Asn Ala Thr Thr Ile 
65                  70                  75                  80  


Ser Ala Gly Ile Asp Gly Gly Gln Pro Trp Arg Arg Thr Phe Gly Ser 
                85                  90                  95      


Asn Val Thr Tyr Thr Asp Trp Arg Arg Asp Asn Phe Tyr Arg Ala Val 
            100                 105                 110         


Gln Gly Leu Thr Lys Gly Ala Arg Arg Val Gly Ile Glu Phe Asp His 
        115                 120                 125             


Val Ser Leu Asp Tyr Arg Gln Leu Leu Gln Asp Ala Leu Pro Gly Val 
    130                 135                 140                 


Glu Leu Val Asp Val Ser Gln Pro Ser Met Trp Met Arg Thr Ile Lys 
145                 150                 155                 160 


Ser Ala Glu Glu Ile Lys Leu Ile Thr Glu Gly Ala Arg Ile Cys Asp 
                165                 170                 175     


Val Gly Gly Tyr Ala Val Ala Gly Ala Val Lys Ala Gly Val Pro Glu 
            180                 185                 190         


His Glu Val Ala Ile Ala Gly Thr Asn Ala Met Ile Arg Glu Ile Ala 
        195                 200                 205             


Lys Ser Phe Pro Phe Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln 
    210                 215                 220                 


Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro Val Thr Asn Arg Val 
225                 230                 235                 240 


Val Gln Ser Gly Asp Ile Leu Ser Leu Asn Thr Phe Pro Met Ile Phe 
                245                 250                 255     


Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Leu Phe Cys Asp His Val Asp 
            260                 265                 270         


Asp Ala Ser Leu Asp Ile Trp Glu Lys Asn Val Ala Val His Arg Arg 
        275                 280                 285             


Gly Leu Glu Leu Met Lys Pro Gly Ala Arg Cys Met Asp Ile Ala Ile 
    290                 295                 300                 


Glu Leu Asn Glu Met Tyr Arg Asp Trp Asp Leu Leu Lys Tyr Arg Ser 
305                 310                 315                 320 


Phe Gly Tyr Gly His Ser Phe Gly Val Leu Ser His Tyr Tyr Gly Arg 
                325                 330                 335     


Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Asp Thr Val Leu Lys Pro 
            340                 345                 350         


Gly Met Val Val Ser Met Glu Pro Met Val Met Ile Pro Glu Gly Gln 
        355                 360                 365             


Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile Leu Val Ile Gly Glu 
    370                 375                 380                 


Asp Gly Ala Glu Asn Ile Thr Gly Phe Pro Phe Gly Pro Glu His Asn 
385                 390                 395                 400 


Ile Val Gly Lys Gly 
                405 


<210>  19
<211>  405
<212>  PRT
<213>  Roseovarius sp


<220>
<221>  mat_peptide
<222>  (1)..(405)
<223>  Mutant creatine amidinohydrolase _Roseovarius sp. A3W1E4 with 
       C175A+C299A substitutions

<400>  19

Met Leu Asp Asp Met Leu His Val Thr Glu Trp His Asn Gly Glu Lys 
1               5                   10                  15      


Glu Phe Ser Pro Phe Ser Asp Asn Glu Met Ala Arg Arg Gln Asn Glu 
            20                  25                  30          


Leu Arg Val Trp Met Ala Asp Asn Asn Val Asp Ala Ala Leu Phe Thr 
        35                  40                  45              


Ser Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp Leu Tyr Cys Tyr Phe 
    50                  55                  60                  


Gly Arg Lys Tyr Gly Met Val Ile Asp Gln Lys Asn Ala Thr Thr Ile 
65                  70                  75                  80  


Ser Ala Gly Ile Asp Gly Gly Gln Pro Trp Arg Arg Thr Phe Gly Ser 
                85                  90                  95      


Asn Val Thr Tyr Thr Asp Trp Arg Arg Asp Asn Phe Tyr Arg Ala Val 
            100                 105                 110         


Gln Gly Leu Thr Lys Gly Ala Arg Arg Val Gly Ile Glu Phe Asp His 
        115                 120                 125             


Val Ser Leu Asp Tyr Arg Gln Leu Leu Gln Asp Ala Leu Pro Gly Val 
    130                 135                 140                 


Glu Leu Val Asp Val Ser Gln Pro Ser Met Trp Met Arg Thr Ile Lys 
145                 150                 155                 160 


Ser Ala Glu Glu Ile Lys Leu Ile Thr Glu Gly Ala Arg Ile Ala Asp 
                165                 170                 175     


Val Gly Gly Tyr Ala Val Ala Gly Ala Val Lys Ala Gly Val Pro Glu 
            180                 185                 190         


His Glu Val Ala Ile Ala Gly Thr Asn Ala Met Ile Arg Glu Ile Ala 
        195                 200                 205             


Lys Ser Phe Pro Phe Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln 
    210                 215                 220                 


Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro Val Thr Asn Arg Val 
225                 230                 235                 240 


Val Gln Ser Gly Asp Ile Leu Ser Leu Asn Thr Phe Pro Met Ile Phe 
                245                 250                 255     


Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Leu Phe Cys Asp His Val Asp 
            260                 265                 270         


Asp Ala Ser Leu Asp Ile Trp Glu Lys Asn Val Ala Val His Arg Arg 
        275                 280                 285             


Gly Leu Glu Leu Met Lys Pro Gly Ala Arg Ala Met Asp Ile Ala Ile 
    290                 295                 300                 


Glu Leu Asn Glu Met Tyr Arg Asp Trp Asp Leu Leu Lys Tyr Arg Ser 
305                 310                 315                 320 


Phe Gly Tyr Gly His Ser Phe Gly Val Leu Ser His Tyr Tyr Gly Arg 
                325                 330                 335     


Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Asp Thr Val Leu Lys Pro 
            340                 345                 350         


Gly Met Val Val Ser Met Glu Pro Met Val Met Ile Pro Glu Gly Gln 
        355                 360                 365             


Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile Leu Val Ile Gly Glu 
    370                 375                 380                 


Asp Gly Ala Glu Asn Ile Thr Gly Phe Pro Phe Gly Pro Glu His Asn 
385                 390                 395                 400 


Ile Val Gly Lys Gly 
                405 


<210>  20
<211>  405
<212>  PRT
<213>  Roseovarius sp.


<220>
<221>  mat_peptide
<222>  (1)..(405)
<223>  Mutant creatine amidinohydrolase _Roseovarius sp. A3W1E4 with 
       C175A+C299A-C268L substitutions

<400>  20

Met Leu Asp Asp Met Leu His Val Thr Glu Trp His Asn Gly Glu Lys 
1               5                   10                  15      


Glu Phe Ser Pro Phe Ser Asp Asn Glu Met Ala Arg Arg Gln Asn Glu 
            20                  25                  30          


Leu Arg Val Trp Met Ala Asp Asn Asn Val Asp Ala Ala Leu Phe Thr 
        35                  40                  45              


Ser Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp Leu Tyr Cys Tyr Phe 
    50                  55                  60                  


Gly Arg Lys Tyr Gly Met Val Ile Asp Gln Lys Asn Ala Thr Thr Ile 
65                  70                  75                  80  


Ser Ala Gly Ile Asp Gly Gly Gln Pro Trp Arg Arg Thr Phe Gly Ser 
                85                  90                  95      


Asn Val Thr Tyr Thr Asp Trp Arg Arg Asp Asn Phe Tyr Arg Ala Val 
            100                 105                 110         


Gln Gly Leu Thr Lys Gly Ala Arg Arg Val Gly Ile Glu Phe Asp His 
        115                 120                 125             


Val Ser Leu Asp Tyr Arg Gln Leu Leu Gln Asp Ala Leu Pro Gly Val 
    130                 135                 140                 


Glu Leu Val Asp Val Ser Gln Pro Ser Met Trp Met Arg Thr Ile Lys 
145                 150                 155                 160 


Ser Ala Glu Glu Ile Lys Leu Ile Thr Glu Gly Ala Arg Ile Ala Asp 
                165                 170                 175     


Val Gly Gly Tyr Ala Val Ala Gly Ala Val Lys Ala Gly Val Pro Glu 
            180                 185                 190         


His Glu Val Ala Ile Ala Gly Thr Asn Ala Met Ile Arg Glu Ile Ala 
        195                 200                 205             


Lys Ser Phe Pro Phe Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln 
    210                 215                 220                 


Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro Val Thr Asn Arg Val 
225                 230                 235                 240 


Val Gln Ser Gly Asp Ile Leu Ser Leu Asn Thr Phe Pro Met Ile Phe 
                245                 250                 255     


Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Leu Phe Leu Asp His Val Asp 
            260                 265                 270         


Asp Ala Ser Leu Asp Ile Trp Glu Lys Asn Val Ala Val His Arg Arg 
        275                 280                 285             


Gly Leu Glu Leu Met Lys Pro Gly Ala Arg Ala Met Asp Ile Ala Ile 
    290                 295                 300                 


Glu Leu Asn Glu Met Tyr Arg Asp Trp Asp Leu Leu Lys Tyr Arg Ser 
305                 310                 315                 320 


Phe Gly Tyr Gly His Ser Phe Gly Val Leu Ser His Tyr Tyr Gly Arg 
                325                 330                 335     


Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Asp Thr Val Leu Lys Pro 
            340                 345                 350         


Gly Met Val Val Ser Met Glu Pro Met Val Met Ile Pro Glu Gly Gln 
        355                 360                 365             


Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile Leu Val Ile Gly Glu 
    370                 375                 380                 


Asp Gly Ala Glu Asn Ile Thr Gly Phe Pro Phe Gly Pro Glu His Asn 
385                 390                 395                 400 


Ile Val Gly Lys Gly 
                405 


<210>  21
<211>  1215
<212>  DNA
<213>  Paracoccus denitrificans


<220>
<221>  CDS
<222>  (1)..(1215)
<223>  ABL69129.1 Paracoccus denitrificans PD1222 gene encoding creatine
       amidinohydrolase [A1B0T5]

<400>  21
atg acc gac gac atg ctt cat gtc atg gaa tgg cat aat ggc gat aag         48
Met Thr Asp Asp Met Leu His Val Met Glu Trp His Asn Gly Asp Lys           
1               5                   10                  15                

gat ttt tcc cct ttc tcg gat gcc gag atg cag cgg cgc cag gac gac         96
Asp Phe Ser Pro Phe Ser Asp Ala Glu Met Gln Arg Arg Gln Asp Asp           
            20                  25                  30                    

atg cgc cgc tgg atg gcc ggg aac ggc gtc gat gcg gca ctg ttc acc        144
Met Arg Arg Trp Met Ala Gly Asn Gly Val Asp Ala Ala Leu Phe Thr           
        35                  40                  45                        

tcg tat cac tgc atc aac tat tat tcg ggc tgg ctc tac tgc tat ttc        192
Ser Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp Leu Tyr Cys Tyr Phe           
    50                  55                  60                            

ggc cgc aaa tac ggc atg gtc atc acc cag gac gcg gcg acc acc atc        240
Gly Arg Lys Tyr Gly Met Val Ile Thr Gln Asp Ala Ala Thr Thr Ile           
65                  70                  75                  80            

agc gcc ggc atc gat ggc ggt cag ccg tgg cgg cgc agc ttt ggc ggc        288
Ser Ala Gly Ile Asp Gly Gly Gln Pro Trp Arg Arg Ser Phe Gly Gly           
                85                  90                  95                

aac gtc acc tat acc gat tgg cgg cgc gac aat tat ttc cgc gcg gtg        336
Asn Val Thr Tyr Thr Asp Trp Arg Arg Asp Asn Tyr Phe Arg Ala Val           
            100                 105                 110                   

cgg cag ctg acc ccc ggc gtc aag cgg ctg gga atc gag ttc gac cat        384
Arg Gln Leu Thr Pro Gly Val Lys Arg Leu Gly Ile Glu Phe Asp His           
        115                 120                 125                       

gtc aac atg gac ttg cgc cgc cag ctt gag gca gcc ctg ccg ggg gtg        432
Val Asn Met Asp Leu Arg Arg Gln Leu Glu Ala Ala Leu Pro Gly Val           
    130                 135                 140                           

gaa ttc gtc gat gtc ggc cag ccc tcg atg tgg atg cgc tcg atc aag        480
Glu Phe Val Asp Val Gly Gln Pro Ser Met Trp Met Arg Ser Ile Lys           
145                 150                 155                 160           

tcg gcc gag gaa cac aag ctg atc cgc gag ggc gcg cgc atc tgc gac        528
Ser Ala Glu Glu His Lys Leu Ile Arg Glu Gly Ala Arg Ile Cys Asp           
                165                 170                 175               

gtg ggc ggc gcg gcg gtg gcg gct gcg gtc aag gcg ggc gtg ccc gag        576
Val Gly Gly Ala Ala Val Ala Ala Ala Val Lys Ala Gly Val Pro Glu           
            180                 185                 190                   

cac gag gtc gcc atc gcc tcg acc aat gcc atg atc cgc gag atc gcc        624
His Glu Val Ala Ile Ala Ser Thr Asn Ala Met Ile Arg Glu Ile Ala           
        195                 200                 205                       

gcc tcc ttc ccc ttc gtc gag ctg atg gat acc tgg acc tgg ttc cag        672
Ala Ser Phe Pro Phe Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln           
    210                 215                 220                           

tcc ggc atc aac acc gac ggg gcg cat aac ccg gtg acg aac aag aag        720
Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro Val Thr Asn Lys Lys           
225                 230                 235                 240           

atc gca tcg ggc gag atc ctg tcg ctg aac tgc ttc ccg atg atc ttc        768
Ile Ala Ser Gly Glu Ile Leu Ser Leu Asn Cys Phe Pro Met Ile Phe           
                245                 250                 255               

ggc tat tat acc gcg ctg gaa cgc acg atg ttt tgc gac agc gtg gac        816
Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Met Phe Cys Asp Ser Val Asp           
            260                 265                 270                   

gat gcc agc ctc gac atc tgg gaa aag aac gtc gcc gtg cat cgc cgg        864
Asp Ala Ser Leu Asp Ile Trp Glu Lys Asn Val Ala Val His Arg Arg           
        275                 280                 285                       

ggc ctg gaa ctg atc aag ccc ggt gcg aaa tgc aac gag atc gca ttg        912
Gly Leu Glu Leu Ile Lys Pro Gly Ala Lys Cys Asn Glu Ile Ala Leu           
    290                 295                 300                           

gag ctc aac gac atg tac cgc cag tgg gat ctg ctg aaa tat cgc agc        960
Glu Leu Asn Asp Met Tyr Arg Gln Trp Asp Leu Leu Lys Tyr Arg Ser           
305                 310                 315                 320           

ttc ggc tat ggc cac tcc ttc ggc gtc ctg agc cac tat tac ggg cgc       1008
Phe Gly Tyr Gly His Ser Phe Gly Val Leu Ser His Tyr Tyr Gly Arg           
                325                 330                 335               

gag gcc ggg gtc gag ctg cgc gag gac atc gag acc gag ctg aag ccc       1056
Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Glu Thr Glu Leu Lys Pro           
            340                 345                 350                   

ggc atg gtg gtc tcg atg gaa ccg atg gtg atg ttg ccc gag ggt gcg       1104
Gly Met Val Val Ser Met Glu Pro Met Val Met Leu Pro Glu Gly Ala           
        355                 360                 365                       

ccc ggc gcg ggc ggc tat cgc gag cat gac atc ctg atc gtg acc gag       1152
Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile Leu Ile Val Thr Glu           
    370                 375                 380                           

gac ggg gcc gat aac atc acc ggg ttc ccc ttc ggc ccc gag cac aac       1200
Asp Gly Ala Asp Asn Ile Thr Gly Phe Pro Phe Gly Pro Glu His Asn           
385                 390                 395                 400           

atc atc cgc aac tga                                                   1215
Ile Ile Arg Asn                                                           
                                                                          


<210>  22
<211>  404
<212>  PRT
<213>  Paracoccus denitrificans

<400>  22

Met Thr Asp Asp Met Leu His Val Met Glu Trp His Asn Gly Asp Lys 
1               5                   10                  15      


Asp Phe Ser Pro Phe Ser Asp Ala Glu Met Gln Arg Arg Gln Asp Asp 
            20                  25                  30          


Met Arg Arg Trp Met Ala Gly Asn Gly Val Asp Ala Ala Leu Phe Thr 
        35                  40                  45              


Ser Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp Leu Tyr Cys Tyr Phe 
    50                  55                  60                  


Gly Arg Lys Tyr Gly Met Val Ile Thr Gln Asp Ala Ala Thr Thr Ile 
65                  70                  75                  80  


Ser Ala Gly Ile Asp Gly Gly Gln Pro Trp Arg Arg Ser Phe Gly Gly 
                85                  90                  95      


Asn Val Thr Tyr Thr Asp Trp Arg Arg Asp Asn Tyr Phe Arg Ala Val 
            100                 105                 110         


Arg Gln Leu Thr Pro Gly Val Lys Arg Leu Gly Ile Glu Phe Asp His 
        115                 120                 125             


Val Asn Met Asp Leu Arg Arg Gln Leu Glu Ala Ala Leu Pro Gly Val 
    130                 135                 140                 


Glu Phe Val Asp Val Gly Gln Pro Ser Met Trp Met Arg Ser Ile Lys 
145                 150                 155                 160 


Ser Ala Glu Glu His Lys Leu Ile Arg Glu Gly Ala Arg Ile Cys Asp 
                165                 170                 175     


Val Gly Gly Ala Ala Val Ala Ala Ala Val Lys Ala Gly Val Pro Glu 
            180                 185                 190         


His Glu Val Ala Ile Ala Ser Thr Asn Ala Met Ile Arg Glu Ile Ala 
        195                 200                 205             


Ala Ser Phe Pro Phe Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln 
    210                 215                 220                 


Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro Val Thr Asn Lys Lys 
225                 230                 235                 240 


Ile Ala Ser Gly Glu Ile Leu Ser Leu Asn Cys Phe Pro Met Ile Phe 
                245                 250                 255     


Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Met Phe Cys Asp Ser Val Asp 
            260                 265                 270         


Asp Ala Ser Leu Asp Ile Trp Glu Lys Asn Val Ala Val His Arg Arg 
        275                 280                 285             


Gly Leu Glu Leu Ile Lys Pro Gly Ala Lys Cys Asn Glu Ile Ala Leu 
    290                 295                 300                 


Glu Leu Asn Asp Met Tyr Arg Gln Trp Asp Leu Leu Lys Tyr Arg Ser 
305                 310                 315                 320 


Phe Gly Tyr Gly His Ser Phe Gly Val Leu Ser His Tyr Tyr Gly Arg 
                325                 330                 335     


Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Glu Thr Glu Leu Lys Pro 
            340                 345                 350         


Gly Met Val Val Ser Met Glu Pro Met Val Met Leu Pro Glu Gly Ala 
        355                 360                 365             


Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile Leu Ile Val Thr Glu 
    370                 375                 380                 


Asp Gly Ala Asp Asn Ile Thr Gly Phe Pro Phe Gly Pro Glu His Asn 
385                 390                 395                 400 


Ile Ile Arg Asn 
                


<210>  23
<211>  404
<212>  PRT
<213>  Paracoccus denitrificans


<220>
<221>  mat_peptide
<222>  (1)..(404)
<223>  Mutant creatine amidinohydrolase _Paracoccus denitrificans A1B0T5
       with C175A+C299A substitutions

<400>  23

Met Thr Asp Asp Met Leu His Val Met Glu Trp His Asn Gly Asp Lys 
1               5                   10                  15      


Asp Phe Ser Pro Phe Ser Asp Ala Glu Met Gln Arg Arg Gln Asp Asp 
            20                  25                  30          


Met Arg Arg Trp Met Ala Gly Asn Gly Val Asp Ala Ala Leu Phe Thr 
        35                  40                  45              


Ser Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp Leu Tyr Cys Tyr Phe 
    50                  55                  60                  


Gly Arg Lys Tyr Gly Met Val Ile Thr Gln Asp Ala Ala Thr Thr Ile 
65                  70                  75                  80  


Ser Ala Gly Ile Asp Gly Gly Gln Pro Trp Arg Arg Ser Phe Gly Gly 
                85                  90                  95      


Asn Val Thr Tyr Thr Asp Trp Arg Arg Asp Asn Tyr Phe Arg Ala Val 
            100                 105                 110         


Arg Gln Leu Thr Pro Gly Val Lys Arg Leu Gly Ile Glu Phe Asp His 
        115                 120                 125             


Val Asn Met Asp Leu Arg Arg Gln Leu Glu Ala Ala Leu Pro Gly Val 
    130                 135                 140                 


Glu Phe Val Asp Val Gly Gln Pro Ser Met Trp Met Arg Ser Ile Lys 
145                 150                 155                 160 


Ser Ala Glu Glu His Lys Leu Ile Arg Glu Gly Ala Arg Ile Ala Asp 
                165                 170                 175     


Val Gly Gly Ala Ala Val Ala Ala Ala Val Lys Ala Gly Val Pro Glu 
            180                 185                 190         


His Glu Val Ala Ile Ala Ser Thr Asn Ala Met Ile Arg Glu Ile Ala 
        195                 200                 205             


Ala Ser Phe Pro Phe Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln 
    210                 215                 220                 


Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro Val Thr Asn Lys Lys 
225                 230                 235                 240 


Ile Ala Ser Gly Glu Ile Leu Ser Leu Asn Cys Phe Pro Met Ile Phe 
                245                 250                 255     


Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Met Phe Cys Asp Ser Val Asp 
            260                 265                 270         


Asp Ala Ser Leu Asp Ile Trp Glu Lys Asn Val Ala Val His Arg Arg 
        275                 280                 285             


Gly Leu Glu Leu Ile Lys Pro Gly Ala Lys Ala Asn Glu Ile Ala Leu 
    290                 295                 300                 


Glu Leu Asn Asp Met Tyr Arg Gln Trp Asp Leu Leu Lys Tyr Arg Ser 
305                 310                 315                 320 


Phe Gly Tyr Gly His Ser Phe Gly Val Leu Ser His Tyr Tyr Gly Arg 
                325                 330                 335     


Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Glu Thr Glu Leu Lys Pro 
            340                 345                 350         


Gly Met Val Val Ser Met Glu Pro Met Val Met Leu Pro Glu Gly Ala 
        355                 360                 365             


Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile Leu Ile Val Thr Glu 
    370                 375                 380                 


Asp Gly Ala Asp Asn Ile Thr Gly Phe Pro Phe Gly Pro Glu His Asn 
385                 390                 395                 400 


Ile Ile Arg Asn 
                


<210>  24
<211>  404
<212>  PRT
<213>  Paracoccus denitrificans


<220>
<221>  mat_peptide
<222>  (1)..(404)
<223>  Mutant creatine amidinohydrolase _Paracoccus denitrificans A1B0T5
       with C175A+C299A+C268L substitutions

<400>  24

Met Thr Asp Asp Met Leu His Val Met Glu Trp His Asn Gly Asp Lys 
1               5                   10                  15      


Asp Phe Ser Pro Phe Ser Asp Ala Glu Met Gln Arg Arg Gln Asp Asp 
            20                  25                  30          


Met Arg Arg Trp Met Ala Gly Asn Gly Val Asp Ala Ala Leu Phe Thr 
        35                  40                  45              


Ser Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp Leu Tyr Cys Tyr Phe 
    50                  55                  60                  


Gly Arg Lys Tyr Gly Met Val Ile Thr Gln Asp Ala Ala Thr Thr Ile 
65                  70                  75                  80  


Ser Ala Gly Ile Asp Gly Gly Gln Pro Trp Arg Arg Ser Phe Gly Gly 
                85                  90                  95      


Asn Val Thr Tyr Thr Asp Trp Arg Arg Asp Asn Tyr Phe Arg Ala Val 
            100                 105                 110         


Arg Gln Leu Thr Pro Gly Val Lys Arg Leu Gly Ile Glu Phe Asp His 
        115                 120                 125             


Val Asn Met Asp Leu Arg Arg Gln Leu Glu Ala Ala Leu Pro Gly Val 
    130                 135                 140                 


Glu Phe Val Asp Val Gly Gln Pro Ser Met Trp Met Arg Ser Ile Lys 
145                 150                 155                 160 


Ser Ala Glu Glu His Lys Leu Ile Arg Glu Gly Ala Arg Ile Ala Asp 
                165                 170                 175     


Val Gly Gly Ala Ala Val Ala Ala Ala Val Lys Ala Gly Val Pro Glu 
            180                 185                 190         


His Glu Val Ala Ile Ala Ser Thr Asn Ala Met Ile Arg Glu Ile Ala 
        195                 200                 205             


Ala Ser Phe Pro Phe Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln 
    210                 215                 220                 


Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro Val Thr Asn Lys Lys 
225                 230                 235                 240 


Ile Ala Ser Gly Glu Ile Leu Ser Leu Asn Cys Phe Pro Met Ile Phe 
                245                 250                 255     


Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Met Phe Leu Asp Ser Val Asp 
            260                 265                 270         


Asp Ala Ser Leu Asp Ile Trp Glu Lys Asn Val Ala Val His Arg Arg 
        275                 280                 285             


Gly Leu Glu Leu Ile Lys Pro Gly Ala Lys Ala Asn Glu Ile Ala Leu 
    290                 295                 300                 


Glu Leu Asn Asp Met Tyr Arg Gln Trp Asp Leu Leu Lys Tyr Arg Ser 
305                 310                 315                 320 


Phe Gly Tyr Gly His Ser Phe Gly Val Leu Ser His Tyr Tyr Gly Arg 
                325                 330                 335     


Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Glu Thr Glu Leu Lys Pro 
            340                 345                 350         


Gly Met Val Val Ser Met Glu Pro Met Val Met Leu Pro Glu Gly Ala 
        355                 360                 365             


Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile Leu Ile Val Thr Glu 
    370                 375                 380                 


Asp Gly Ala Asp Asn Ile Thr Gly Phe Pro Phe Gly Pro Glu His Asn 
385                 390                 395                 400 


Ile Ile Arg Asn 
                


<210>  25
<211>  1215
<212>  DNA
<213>  Rubellimicrobium mesophilum


<220>
<221>  CDS
<222>  (1)..(1215)
<223>  EYD77030.1 Rubellimicrobium mesophilum DSM 19309 gene encoding 
       creatine amidinohydrolase [A0A017HRV0]

<400>  25
atg gcc gag gac atg ctg cac gta atg gga tgg cac aac ggg gat aag         48
Met Ala Glu Asp Met Leu His Val Met Gly Trp His Asn Gly Asp Lys           
1               5                   10                  15                

gaa tat tca ccc ttc tcc gag gcc gag atg agc cga cgg caa ggc gat         96
Glu Tyr Ser Pro Phe Ser Glu Ala Glu Met Ser Arg Arg Gln Gly Asp           
            20                  25                  30                    

atc cgg aca tgg atg gcc gag aac gac gtc gat gcc gcg ctg ttc acg        144
Ile Arg Thr Trp Met Ala Glu Asn Asp Val Asp Ala Ala Leu Phe Thr           
        35                  40                  45                        

tcc tat cat tgc atc aat tac tat tcc ggc tgg ctc tat tgc caa ttc        192
Ser Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp Leu Tyr Cys Gln Phe           
    50                  55                  60                            

ggc cgg aga tac gga atg atc gtc acc cag gac agg gcg ctg acg gtg        240
Gly Arg Arg Tyr Gly Met Ile Val Thr Gln Asp Arg Ala Leu Thr Val           
65                  70                  75                  80            

tcc gcc ggg atc gac ggc ggc cag ccc tgg cga cgg agc ttc ggc gac        288
Ser Ala Gly Ile Asp Gly Gly Gln Pro Trp Arg Arg Ser Phe Gly Asp           
                85                  90                  95                

aac atc acc tac acc gat tgg cgg cgc gac aac ttc tac cgt gcg gtg        336
Asn Ile Thr Tyr Thr Asp Trp Arg Arg Asp Asn Phe Tyr Arg Ala Val           
            100                 105                 110                   

cgc cag aac ctg ccg ggc gtg agg cgc ctc gga atc gag ttc gac cac        384
Arg Gln Asn Leu Pro Gly Val Arg Arg Leu Gly Ile Glu Phe Asp His           
        115                 120                 125                       

gtc tcg ctc gac ttc cga cgt cag ctc ggc gag gcg ctg ccg ggc gtg        432
Val Ser Leu Asp Phe Arg Arg Gln Leu Gly Glu Ala Leu Pro Gly Val           
    130                 135                 140                           

gag ttc gtg gac gtg ggc cag ccc tcg atg tgg atg cgg acc atc aag        480
Glu Phe Val Asp Val Gly Gln Pro Ser Met Trp Met Arg Thr Ile Lys           
145                 150                 155                 160           

tcc gag gag gag cgg agg ctc atc cgc gag ggc gcg cgg gtc tgc gac        528
Ser Glu Glu Glu Arg Arg Leu Ile Arg Glu Gly Ala Arg Val Cys Asp           
                165                 170                 175               

gtc ggt ggc gcg gcg gtg gcg gag gcg gtg agg gcg ggc gtg ccc gag        576
Val Gly Gly Ala Ala Val Ala Glu Ala Val Arg Ala Gly Val Pro Glu           
            180                 185                 190                   

cat gag gtc gcc atc gcc tcg acc aat gcg atg atc cgc gag atc gcg        624
His Glu Val Ala Ile Ala Ser Thr Asn Ala Met Ile Arg Glu Ile Ala           
        195                 200                 205                       

agg tcg ttc ccc tat gtg gag ctg atg gac acc tgg acc tgg ttc cag        672
Arg Ser Phe Pro Tyr Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln           
    210                 215                 220                           

tcg ggc atc aac acc gat ggc gcg cac aac ccg gtc acc aat cgc gtg        720
Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro Val Thr Asn Arg Val           
225                 230                 235                 240           

gtc cag tcg ggg gac atc ctg tcg ctc aac tgc ttc ccg atg atc ttc        768
Val Gln Ser Gly Asp Ile Leu Ser Leu Asn Cys Phe Pro Met Ile Phe           
                245                 250                 255               

ggc tac tac acc gcg ctg gag cgg acg atg ttc tgc gac cac gtg gac        816
Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Met Phe Cys Asp His Val Asp           
            260                 265                 270                   

gac gcg agc ctc gac atc tgg gag aag aac gtg gcc gtc cac cgg cgg        864
Asp Ala Ser Leu Asp Ile Trp Glu Lys Asn Val Ala Val His Arg Arg           
        275                 280                 285                       

ggg ctg gag ctg atc cga ccc ggg gcg aag tgc aac gag atc gcc gcc        912
Gly Leu Glu Leu Ile Arg Pro Gly Ala Lys Cys Asn Glu Ile Ala Ala           
    290                 295                 300                           

gag ctg aac gag atg tac cgg cag tgg gac ctg ctg cag tat cgc agc        960
Glu Leu Asn Glu Met Tyr Arg Gln Trp Asp Leu Leu Gln Tyr Arg Ser           
305                 310                 315                 320           

ttc ggc tac ggg cat tcc ttc ggg gtg ctc tgc cac tac tac ggc cgc       1008
Phe Gly Tyr Gly His Ser Phe Gly Val Leu Cys His Tyr Tyr Gly Arg           
                325                 330                 335               

gag gcg ggc gtg gag ctg cgg gag gac atc gac acc gag ctg aag ccc       1056
Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Asp Thr Glu Leu Lys Pro           
            340                 345                 350                   

ggg atg gtc gtc tcc atg gag ccc atg gtg atg atc ccg aac ggc aac       1104
Gly Met Val Val Ser Met Glu Pro Met Val Met Ile Pro Asn Gly Asn           
        355                 360                 365                       

ccc ggg gcg ggc ggc tac cgc gag cac gac ata ctg atc gtg acc gag       1152
Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile Leu Ile Val Thr Glu           
    370                 375                 380                           

gat ggg gcg gag aac atc acc aag ttc ccc ttc ggc ccc gag cac aac       1200
Asp Gly Ala Glu Asn Ile Thr Lys Phe Pro Phe Gly Pro Glu His Asn           
385                 390                 395                 400           

gtc atc cgc aac tga                                                   1215
Val Ile Arg Asn                                                           
                                                                          


<210>  26
<211>  404
<212>  PRT
<213>  Rubellimicrobium mesophilum

<400>  26

Met Ala Glu Asp Met Leu His Val Met Gly Trp His Asn Gly Asp Lys 
1               5                   10                  15      


Glu Tyr Ser Pro Phe Ser Glu Ala Glu Met Ser Arg Arg Gln Gly Asp 
            20                  25                  30          


Ile Arg Thr Trp Met Ala Glu Asn Asp Val Asp Ala Ala Leu Phe Thr 
        35                  40                  45              


Ser Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp Leu Tyr Cys Gln Phe 
    50                  55                  60                  


Gly Arg Arg Tyr Gly Met Ile Val Thr Gln Asp Arg Ala Leu Thr Val 
65                  70                  75                  80  


Ser Ala Gly Ile Asp Gly Gly Gln Pro Trp Arg Arg Ser Phe Gly Asp 
                85                  90                  95      


Asn Ile Thr Tyr Thr Asp Trp Arg Arg Asp Asn Phe Tyr Arg Ala Val 
            100                 105                 110         


Arg Gln Asn Leu Pro Gly Val Arg Arg Leu Gly Ile Glu Phe Asp His 
        115                 120                 125             


Val Ser Leu Asp Phe Arg Arg Gln Leu Gly Glu Ala Leu Pro Gly Val 
    130                 135                 140                 


Glu Phe Val Asp Val Gly Gln Pro Ser Met Trp Met Arg Thr Ile Lys 
145                 150                 155                 160 


Ser Glu Glu Glu Arg Arg Leu Ile Arg Glu Gly Ala Arg Val Cys Asp 
                165                 170                 175     


Val Gly Gly Ala Ala Val Ala Glu Ala Val Arg Ala Gly Val Pro Glu 
            180                 185                 190         


His Glu Val Ala Ile Ala Ser Thr Asn Ala Met Ile Arg Glu Ile Ala 
        195                 200                 205             


Arg Ser Phe Pro Tyr Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln 
    210                 215                 220                 


Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro Val Thr Asn Arg Val 
225                 230                 235                 240 


Val Gln Ser Gly Asp Ile Leu Ser Leu Asn Cys Phe Pro Met Ile Phe 
                245                 250                 255     


Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Met Phe Cys Asp His Val Asp 
            260                 265                 270         


Asp Ala Ser Leu Asp Ile Trp Glu Lys Asn Val Ala Val His Arg Arg 
        275                 280                 285             


Gly Leu Glu Leu Ile Arg Pro Gly Ala Lys Cys Asn Glu Ile Ala Ala 
    290                 295                 300                 


Glu Leu Asn Glu Met Tyr Arg Gln Trp Asp Leu Leu Gln Tyr Arg Ser 
305                 310                 315                 320 


Phe Gly Tyr Gly His Ser Phe Gly Val Leu Cys His Tyr Tyr Gly Arg 
                325                 330                 335     


Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Asp Thr Glu Leu Lys Pro 
            340                 345                 350         


Gly Met Val Val Ser Met Glu Pro Met Val Met Ile Pro Asn Gly Asn 
        355                 360                 365             


Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile Leu Ile Val Thr Glu 
    370                 375                 380                 


Asp Gly Ala Glu Asn Ile Thr Lys Phe Pro Phe Gly Pro Glu His Asn 
385                 390                 395                 400 


Val Ile Arg Asn 
                


<210>  27
<211>  404
<212>  PRT
<213>  Rubellimicrobium mesophilum


<220>
<221>  mat_peptide
<222>  (1)..(404)
<223>  Mutant creatine amidinohydrolase _Rubellimicrobium mesophilum 
       A0A017HRV0 with C175A+C299A substitutions

<400>  27

Met Ala Glu Asp Met Leu His Val Met Gly Trp His Asn Gly Asp Lys 
1               5                   10                  15      


Glu Tyr Ser Pro Phe Ser Glu Ala Glu Met Ser Arg Arg Gln Gly Asp 
            20                  25                  30          


Ile Arg Thr Trp Met Ala Glu Asn Asp Val Asp Ala Ala Leu Phe Thr 
        35                  40                  45              


Ser Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp Leu Tyr Cys Gln Phe 
    50                  55                  60                  


Gly Arg Arg Tyr Gly Met Ile Val Thr Gln Asp Arg Ala Leu Thr Val 
65                  70                  75                  80  


Ser Ala Gly Ile Asp Gly Gly Gln Pro Trp Arg Arg Ser Phe Gly Asp 
                85                  90                  95      


Asn Ile Thr Tyr Thr Asp Trp Arg Arg Asp Asn Phe Tyr Arg Ala Val 
            100                 105                 110         


Arg Gln Asn Leu Pro Gly Val Arg Arg Leu Gly Ile Glu Phe Asp His 
        115                 120                 125             


Val Ser Leu Asp Phe Arg Arg Gln Leu Gly Glu Ala Leu Pro Gly Val 
    130                 135                 140                 


Glu Phe Val Asp Val Gly Gln Pro Ser Met Trp Met Arg Thr Ile Lys 
145                 150                 155                 160 


Ser Glu Glu Glu Arg Arg Leu Ile Arg Glu Gly Ala Arg Val Ala Asp 
                165                 170                 175     


Val Gly Gly Ala Ala Val Ala Glu Ala Val Arg Ala Gly Val Pro Glu 
            180                 185                 190         


His Glu Val Ala Ile Ala Ser Thr Asn Ala Met Ile Arg Glu Ile Ala 
        195                 200                 205             


Arg Ser Phe Pro Tyr Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln 
    210                 215                 220                 


Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro Val Thr Asn Arg Val 
225                 230                 235                 240 


Val Gln Ser Gly Asp Ile Leu Ser Leu Asn Cys Phe Pro Met Ile Phe 
                245                 250                 255     


Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Met Phe Cys Asp His Val Asp 
            260                 265                 270         


Asp Ala Ser Leu Asp Ile Trp Glu Lys Asn Val Ala Val His Arg Arg 
        275                 280                 285             


Gly Leu Glu Leu Ile Arg Pro Gly Ala Lys Ala Asn Glu Ile Ala Ala 
    290                 295                 300                 


Glu Leu Asn Glu Met Tyr Arg Gln Trp Asp Leu Leu Gln Tyr Arg Ser 
305                 310                 315                 320 


Phe Gly Tyr Gly His Ser Phe Gly Val Leu Cys His Tyr Tyr Gly Arg 
                325                 330                 335     


Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Asp Thr Glu Leu Lys Pro 
            340                 345                 350         


Gly Met Val Val Ser Met Glu Pro Met Val Met Ile Pro Asn Gly Asn 
        355                 360                 365             


Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile Leu Ile Val Thr Glu 
    370                 375                 380                 


Asp Gly Ala Glu Asn Ile Thr Lys Phe Pro Phe Gly Pro Glu His Asn 
385                 390                 395                 400 


Val Ile Arg Asn 
                


<210>  28
<211>  404
<212>  PRT
<213>  Rubellimicrobium mesophilum


<220>
<221>  mat_peptide
<222>  (1)..(404)
<223>  Mutant creatine amidinohydrolase _Rubellimicrobium mesophilum 
       A0A017HRV0 with C175A+C299A+C268L substitutions

<400>  28

Met Ala Glu Asp Met Leu His Val Met Gly Trp His Asn Gly Asp Lys 
1               5                   10                  15      


Glu Tyr Ser Pro Phe Ser Glu Ala Glu Met Ser Arg Arg Gln Gly Asp 
            20                  25                  30          


Ile Arg Thr Trp Met Ala Glu Asn Asp Val Asp Ala Ala Leu Phe Thr 
        35                  40                  45              


Ser Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp Leu Tyr Cys Gln Phe 
    50                  55                  60                  


Gly Arg Arg Tyr Gly Met Ile Val Thr Gln Asp Arg Ala Leu Thr Val 
65                  70                  75                  80  


Ser Ala Gly Ile Asp Gly Gly Gln Pro Trp Arg Arg Ser Phe Gly Asp 
                85                  90                  95      


Asn Ile Thr Tyr Thr Asp Trp Arg Arg Asp Asn Phe Tyr Arg Ala Val 
            100                 105                 110         


Arg Gln Asn Leu Pro Gly Val Arg Arg Leu Gly Ile Glu Phe Asp His 
        115                 120                 125             


Val Ser Leu Asp Phe Arg Arg Gln Leu Gly Glu Ala Leu Pro Gly Val 
    130                 135                 140                 


Glu Phe Val Asp Val Gly Gln Pro Ser Met Trp Met Arg Thr Ile Lys 
145                 150                 155                 160 


Ser Glu Glu Glu Arg Arg Leu Ile Arg Glu Gly Ala Arg Val Ala Asp 
                165                 170                 175     


Val Gly Gly Ala Ala Val Ala Glu Ala Val Arg Ala Gly Val Pro Glu 
            180                 185                 190         


His Glu Val Ala Ile Ala Ser Thr Asn Ala Met Ile Arg Glu Ile Ala 
        195                 200                 205             


Arg Ser Phe Pro Tyr Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln 
    210                 215                 220                 


Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro Val Thr Asn Arg Val 
225                 230                 235                 240 


Val Gln Ser Gly Asp Ile Leu Ser Leu Asn Cys Phe Pro Met Ile Phe 
                245                 250                 255     


Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Met Phe Leu Asp His Val Asp 
            260                 265                 270         


Asp Ala Ser Leu Asp Ile Trp Glu Lys Asn Val Ala Val His Arg Arg 
        275                 280                 285             


Gly Leu Glu Leu Ile Arg Pro Gly Ala Lys Ala Asn Glu Ile Ala Ala 
    290                 295                 300                 


Glu Leu Asn Glu Met Tyr Arg Gln Trp Asp Leu Leu Gln Tyr Arg Ser 
305                 310                 315                 320 


Phe Gly Tyr Gly His Ser Phe Gly Val Leu Cys His Tyr Tyr Gly Arg 
                325                 330                 335     


Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Asp Thr Glu Leu Lys Pro 
            340                 345                 350         


Gly Met Val Val Ser Met Glu Pro Met Val Met Ile Pro Asn Gly Asn 
        355                 360                 365             


Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile Leu Ile Val Thr Glu 
    370                 375                 380                 


Asp Gly Ala Glu Asn Ile Thr Lys Phe Pro Phe Gly Pro Glu His Asn 
385                 390                 395                 400 


Val Ile Arg Asn 
                


<210>  29
<211>  1215
<212>  DNA
<213>  Loktanella vestfoldensis


<220>
<221>  CDS
<222>  (1)..(1215)
<223>  EAQ08246.1 Loktanella vestfoldensis SKA53 gene encoding creatine 
       amidinohydrolase [A3V128]

<400>  29
atg gac gac atg ctc cac gtc atg gaa tgg cac aac ggc gag aag gag         48
Met Asp Asp Met Leu His Val Met Glu Trp His Asn Gly Glu Lys Glu           
1               5                   10                  15                

ttc tcg ccc ttt tca gat aac gaa atg gcc cgt cgc cag aac gaa ttg         96
Phe Ser Pro Phe Ser Asp Asn Glu Met Ala Arg Arg Gln Asn Glu Leu           
            20                  25                  30                    

cgc gac tgg atg ggc aag aac gat gtc gat gca tca ctc ttc acc tct        144
Arg Asp Trp Met Gly Lys Asn Asp Val Asp Ala Ser Leu Phe Thr Ser           
        35                  40                  45                        

tat cac tgc atc aac tat tat agc gga tgg ctg tac tgc tat ttc ggt        192
Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp Leu Tyr Cys Tyr Phe Gly           
    50                  55                  60                            

cgt aaa tac ggc atg gtc atc gac cag aag aac gcc act acg atc tct        240
Arg Lys Tyr Gly Met Val Ile Asp Gln Lys Asn Ala Thr Thr Ile Ser           
65                  70                  75                  80            

gcc ggc atc gac ggc ggc caa ccc ttc cgt cgc agc ttc ggc aac aac        288
Ala Gly Ile Asp Gly Gly Gln Pro Phe Arg Arg Ser Phe Gly Asn Asn           
                85                  90                  95                

atc acg tat act gac tgg cgt cgt gac aac ttc tac cgc gcg atc cag        336
Ile Thr Tyr Thr Asp Trp Arg Arg Asp Asn Phe Tyr Arg Ala Ile Gln           
            100                 105                 110                   

caa ctg aca ccg ggt gcc aag cgc atc ggt atc gaa ttc gac cac gtg        384
Gln Leu Thr Pro Gly Ala Lys Arg Ile Gly Ile Glu Phe Asp His Val           
        115                 120                 125                       

tcg ctg gaa tac cgc caa ctg ctt cag gat gcc ctg ccg ggc gtc gag        432
Ser Leu Glu Tyr Arg Gln Leu Leu Gln Asp Ala Leu Pro Gly Val Glu           
    130                 135                 140                           

ttc gtt gac gtc ggt cag ccc gcg atg tgg atg cgg acg atc aag tcg        480
Phe Val Asp Val Gly Gln Pro Ala Met Trp Met Arg Thr Ile Lys Ser           
145                 150                 155                 160           

gcc gaa gaa atc aag ctg atc aaa gaa ggc gcc cgc gtc gcc gac gtt        528
Ala Glu Glu Ile Lys Leu Ile Lys Glu Gly Ala Arg Val Ala Asp Val           
                165                 170                 175               

ggc ggt gct gct gtg gcc gct gcg gtc aaa gcc ggt gtt ccc gaa cac        576
Gly Gly Ala Ala Val Ala Ala Ala Val Lys Ala Gly Val Pro Glu His           
            180                 185                 190                   

gaa gtc gcc att gcc agt acc aat gcg atg atc cgc gag att gcc aac        624
Glu Val Ala Ile Ala Ser Thr Asn Ala Met Ile Arg Glu Ile Ala Asn           
        195                 200                 205                       

tct ttc ccc ttc gtc gaa ttg atg gac acc tgg acc tgg ttc cag tcc        672
Ser Phe Pro Phe Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln Ser           
    210                 215                 220                           

ggc att aac acc gac ggc gcg cac aac ccg gta acg aac aag aaa gtg        720
Gly Ile Asn Thr Asp Gly Ala His Asn Pro Val Thr Asn Lys Lys Val           
225                 230                 235                 240           

cag tcg ggt gaa atc ctc agc ctc aac act ttc ccg atg atc ttt ggc        768
Gln Ser Gly Glu Ile Leu Ser Leu Asn Thr Phe Pro Met Ile Phe Gly           
                245                 250                 255               

tac tac acg gcg ctg gaa cgc aca ctg ttc tgc gac cac gtt gat gat        816
Tyr Tyr Thr Ala Leu Glu Arg Thr Leu Phe Cys Asp His Val Asp Asp           
            260                 265                 270                   

gca agc ctc gat atc tgg gag aag aac gtc aag gtc cac gag cgt gga        864
Ala Ser Leu Asp Ile Trp Glu Lys Asn Val Lys Val His Glu Arg Gly           
        275                 280                 285                       

ctc gag ttg atc aag ccg ggc gcg cgc tgc atg gat atc gcg atc gag        912
Leu Glu Leu Ile Lys Pro Gly Ala Arg Cys Met Asp Ile Ala Ile Glu           
    290                 295                 300                           

ctc aac gag atg tat cgc gaa tgg gac ctg ctg aag tat cgt tcg ttc        960
Leu Asn Glu Met Tyr Arg Glu Trp Asp Leu Leu Lys Tyr Arg Ser Phe           
305                 310                 315                 320           

gga tac ggc cac agc ttt ggc gtt ctg agc cat tac tat ggc cgc gag       1008
Gly Tyr Gly His Ser Phe Gly Val Leu Ser His Tyr Tyr Gly Arg Glu           
                325                 330                 335               

gct ggc gta gaa ctt cgc gaa gac att gaa acg gag ctc aag cca gga       1056
Ala Gly Val Glu Leu Arg Glu Asp Ile Glu Thr Glu Leu Lys Pro Gly           
            340                 345                 350                   

atg gtc gtg tcc atg gag ccc atg gtc atg atc ccg gaa ggt cag cct       1104
Met Val Val Ser Met Glu Pro Met Val Met Ile Pro Glu Gly Gln Pro           
        355                 360                 365                       

ggc gct ggc ggc tac cgt gag cat gac atc cta gtg atc ggc gag gac       1152
Gly Ala Gly Gly Tyr Arg Glu His Asp Ile Leu Val Ile Gly Glu Asp           
    370                 375                 380                           

aac aca gtc gag aac atc acc gga ttc cca ttc ggc ccc gag cac aat       1200
Asn Thr Val Glu Asn Ile Thr Gly Phe Pro Phe Gly Pro Glu His Asn           
385                 390                 395                 400           

gtc atc aag aac taa                                                   1215
Val Ile Lys Asn                                                           
                                                                          


<210>  30
<211>  404
<212>  PRT
<213>  Loktanella vestfoldensis

<400>  30

Met Asp Asp Met Leu His Val Met Glu Trp His Asn Gly Glu Lys Glu 
1               5                   10                  15      


Phe Ser Pro Phe Ser Asp Asn Glu Met Ala Arg Arg Gln Asn Glu Leu 
            20                  25                  30          


Arg Asp Trp Met Gly Lys Asn Asp Val Asp Ala Ser Leu Phe Thr Ser 
        35                  40                  45              


Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp Leu Tyr Cys Tyr Phe Gly 
    50                  55                  60                  


Arg Lys Tyr Gly Met Val Ile Asp Gln Lys Asn Ala Thr Thr Ile Ser 
65                  70                  75                  80  


Ala Gly Ile Asp Gly Gly Gln Pro Phe Arg Arg Ser Phe Gly Asn Asn 
                85                  90                  95      


Ile Thr Tyr Thr Asp Trp Arg Arg Asp Asn Phe Tyr Arg Ala Ile Gln 
            100                 105                 110         


Gln Leu Thr Pro Gly Ala Lys Arg Ile Gly Ile Glu Phe Asp His Val 
        115                 120                 125             


Ser Leu Glu Tyr Arg Gln Leu Leu Gln Asp Ala Leu Pro Gly Val Glu 
    130                 135                 140                 


Phe Val Asp Val Gly Gln Pro Ala Met Trp Met Arg Thr Ile Lys Ser 
145                 150                 155                 160 


Ala Glu Glu Ile Lys Leu Ile Lys Glu Gly Ala Arg Val Ala Asp Val 
                165                 170                 175     


Gly Gly Ala Ala Val Ala Ala Ala Val Lys Ala Gly Val Pro Glu His 
            180                 185                 190         


Glu Val Ala Ile Ala Ser Thr Asn Ala Met Ile Arg Glu Ile Ala Asn 
        195                 200                 205             


Ser Phe Pro Phe Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln Ser 
    210                 215                 220                 


Gly Ile Asn Thr Asp Gly Ala His Asn Pro Val Thr Asn Lys Lys Val 
225                 230                 235                 240 


Gln Ser Gly Glu Ile Leu Ser Leu Asn Thr Phe Pro Met Ile Phe Gly 
                245                 250                 255     


Tyr Tyr Thr Ala Leu Glu Arg Thr Leu Phe Cys Asp His Val Asp Asp 
            260                 265                 270         


Ala Ser Leu Asp Ile Trp Glu Lys Asn Val Lys Val His Glu Arg Gly 
        275                 280                 285             


Leu Glu Leu Ile Lys Pro Gly Ala Arg Cys Met Asp Ile Ala Ile Glu 
    290                 295                 300                 


Leu Asn Glu Met Tyr Arg Glu Trp Asp Leu Leu Lys Tyr Arg Ser Phe 
305                 310                 315                 320 


Gly Tyr Gly His Ser Phe Gly Val Leu Ser His Tyr Tyr Gly Arg Glu 
                325                 330                 335     


Ala Gly Val Glu Leu Arg Glu Asp Ile Glu Thr Glu Leu Lys Pro Gly 
            340                 345                 350         


Met Val Val Ser Met Glu Pro Met Val Met Ile Pro Glu Gly Gln Pro 
        355                 360                 365             


Gly Ala Gly Gly Tyr Arg Glu His Asp Ile Leu Val Ile Gly Glu Asp 
    370                 375                 380                 


Asn Thr Val Glu Asn Ile Thr Gly Phe Pro Phe Gly Pro Glu His Asn 
385                 390                 395                 400 


Val Ile Lys Asn 
                


<210>  31
<211>  404
<212>  PRT
<213>  Loktanella vestfoldensis


<220>
<221>  mat_peptide
<222>  (1)..(404)
<223>  Mutant creatine amidinohydrolase _Loktanella vestfoldensis  
       A3V128 with C298A substitution

<400>  31

Met Asp Asp Met Leu His Val Met Glu Trp His Asn Gly Glu Lys Glu 
1               5                   10                  15      


Phe Ser Pro Phe Ser Asp Asn Glu Met Ala Arg Arg Gln Asn Glu Leu 
            20                  25                  30          


Arg Asp Trp Met Gly Lys Asn Asp Val Asp Ala Ser Leu Phe Thr Ser 
        35                  40                  45              


Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp Leu Tyr Cys Tyr Phe Gly 
    50                  55                  60                  


Arg Lys Tyr Gly Met Val Ile Asp Gln Lys Asn Ala Thr Thr Ile Ser 
65                  70                  75                  80  


Ala Gly Ile Asp Gly Gly Gln Pro Phe Arg Arg Ser Phe Gly Asn Asn 
                85                  90                  95      


Ile Thr Tyr Thr Asp Trp Arg Arg Asp Asn Phe Tyr Arg Ala Ile Gln 
            100                 105                 110         


Gln Leu Thr Pro Gly Ala Lys Arg Ile Gly Ile Glu Phe Asp His Val 
        115                 120                 125             


Ser Leu Glu Tyr Arg Gln Leu Leu Gln Asp Ala Leu Pro Gly Val Glu 
    130                 135                 140                 


Phe Val Asp Val Gly Gln Pro Ala Met Trp Met Arg Thr Ile Lys Ser 
145                 150                 155                 160 


Ala Glu Glu Ile Lys Leu Ile Lys Glu Gly Ala Arg Val Ala Asp Val 
                165                 170                 175     


Gly Gly Ala Ala Val Ala Ala Ala Val Lys Ala Gly Val Pro Glu His 
            180                 185                 190         


Glu Val Ala Ile Ala Ser Thr Asn Ala Met Ile Arg Glu Ile Ala Asn 
        195                 200                 205             


Ser Phe Pro Phe Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln Ser 
    210                 215                 220                 


Gly Ile Asn Thr Asp Gly Ala His Asn Pro Val Thr Asn Lys Lys Val 
225                 230                 235                 240 


Gln Ser Gly Glu Ile Leu Ser Leu Asn Thr Phe Pro Met Ile Phe Gly 
                245                 250                 255     


Tyr Tyr Thr Ala Leu Glu Arg Thr Leu Phe Cys Asp His Val Asp Asp 
            260                 265                 270         


Ala Ser Leu Asp Ile Trp Glu Lys Asn Val Lys Val His Glu Arg Gly 
        275                 280                 285             


Leu Glu Leu Ile Lys Pro Gly Ala Arg Ala Met Asp Ile Ala Ile Glu 
    290                 295                 300                 


Leu Asn Glu Met Tyr Arg Glu Trp Asp Leu Leu Lys Tyr Arg Ser Phe 
305                 310                 315                 320 


Gly Tyr Gly His Ser Phe Gly Val Leu Ser His Tyr Tyr Gly Arg Glu 
                325                 330                 335     


Ala Gly Val Glu Leu Arg Glu Asp Ile Glu Thr Glu Leu Lys Pro Gly 
            340                 345                 350         


Met Val Val Ser Met Glu Pro Met Val Met Ile Pro Glu Gly Gln Pro 
        355                 360                 365             


Gly Ala Gly Gly Tyr Arg Glu His Asp Ile Leu Val Ile Gly Glu Asp 
    370                 375                 380                 


Asn Thr Val Glu Asn Ile Thr Gly Phe Pro Phe Gly Pro Glu His Asn 
385                 390                 395                 400 


Val Ile Lys Asn 
                


<210>  32
<211>  404
<212>  PRT
<213>  Loktanella vestfoldensis


<220>
<221>  mat_peptide
<222>  (1)..(404)
<223>  Mutant creatine amidinohydrolase _Loktanella vestfoldensis  
       A3V128 with C298A +C267L substitutions

<400>  32

Met Asp Asp Met Leu His Val Met Glu Trp His Asn Gly Glu Lys Glu 
1               5                   10                  15      


Phe Ser Pro Phe Ser Asp Asn Glu Met Ala Arg Arg Gln Asn Glu Leu 
            20                  25                  30          


Arg Asp Trp Met Gly Lys Asn Asp Val Asp Ala Ser Leu Phe Thr Ser 
        35                  40                  45              


Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp Leu Tyr Cys Tyr Phe Gly 
    50                  55                  60                  


Arg Lys Tyr Gly Met Val Ile Asp Gln Lys Asn Ala Thr Thr Ile Ser 
65                  70                  75                  80  


Ala Gly Ile Asp Gly Gly Gln Pro Phe Arg Arg Ser Phe Gly Asn Asn 
                85                  90                  95      


Ile Thr Tyr Thr Asp Trp Arg Arg Asp Asn Phe Tyr Arg Ala Ile Gln 
            100                 105                 110         


Gln Leu Thr Pro Gly Ala Lys Arg Ile Gly Ile Glu Phe Asp His Val 
        115                 120                 125             


Ser Leu Glu Tyr Arg Gln Leu Leu Gln Asp Ala Leu Pro Gly Val Glu 
    130                 135                 140                 


Phe Val Asp Val Gly Gln Pro Ala Met Trp Met Arg Thr Ile Lys Ser 
145                 150                 155                 160 


Ala Glu Glu Ile Lys Leu Ile Lys Glu Gly Ala Arg Val Ala Asp Val 
                165                 170                 175     


Gly Gly Ala Ala Val Ala Ala Ala Val Lys Ala Gly Val Pro Glu His 
            180                 185                 190         


Glu Val Ala Ile Ala Ser Thr Asn Ala Met Ile Arg Glu Ile Ala Asn 
        195                 200                 205             


Ser Phe Pro Phe Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln Ser 
    210                 215                 220                 


Gly Ile Asn Thr Asp Gly Ala His Asn Pro Val Thr Asn Lys Lys Val 
225                 230                 235                 240 


Gln Ser Gly Glu Ile Leu Ser Leu Asn Thr Phe Pro Met Ile Phe Gly 
                245                 250                 255     


Tyr Tyr Thr Ala Leu Glu Arg Thr Leu Phe Leu Asp His Val Asp Asp 
            260                 265                 270         


Ala Ser Leu Asp Ile Trp Glu Lys Asn Val Lys Val His Glu Arg Gly 
        275                 280                 285             


Leu Glu Leu Ile Lys Pro Gly Ala Arg Ala Met Asp Ile Ala Ile Glu 
    290                 295                 300                 


Leu Asn Glu Met Tyr Arg Glu Trp Asp Leu Leu Lys Tyr Arg Ser Phe 
305                 310                 315                 320 


Gly Tyr Gly His Ser Phe Gly Val Leu Ser His Tyr Tyr Gly Arg Glu 
                325                 330                 335     


Ala Gly Val Glu Leu Arg Glu Asp Ile Glu Thr Glu Leu Lys Pro Gly 
            340                 345                 350         


Met Val Val Ser Met Glu Pro Met Val Met Ile Pro Glu Gly Gln Pro 
        355                 360                 365             


Gly Ala Gly Gly Tyr Arg Glu His Asp Ile Leu Val Ile Gly Glu Asp 
    370                 375                 380                 


Asn Thr Val Glu Asn Ile Thr Gly Phe Pro Phe Gly Pro Glu His Asn 
385                 390                 395                 400 


Val Ile Lys Asn 
                


<210>  33
<211>  1230
<212>  DNA
<213>  Lutibaculum baratangense


<220>
<221>  CDS
<222>  (1)..(1230)
<223>  ESR24399.1 Lutibaculum baratangense AMV1 gene encoding creatine 
       amidinohydrolase [V4RGE5]

<400>  33
atg ctg gac aag acg atc ctc gac gac atg gtg cac gtc acg gag tgg         48
Met Leu Asp Lys Thr Ile Leu Asp Asp Met Val His Val Thr Glu Trp           
1               5                   10                  15                

cac aac ggc gaa aag gaa ttc ctg ccg ttc tcc gac gcc gag atg agc         96
His Asn Gly Glu Lys Glu Phe Leu Pro Phe Ser Asp Ala Glu Met Ser           
            20                  25                  30                    

cgg cga cag gac gac gtg cgc agt tgg atg gga gcg aat aac gtc gat        144
Arg Arg Gln Asp Asp Val Arg Ser Trp Met Gly Ala Asn Asn Val Asp           
        35                  40                  45                        

gcg gcg ctc ttc acc tcg tat cac tgc atc aac tac tat tcg ggc tgg        192
Ala Ala Leu Phe Thr Ser Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp           
    50                  55                  60                            

ctc tac tgc tat ttc ggc cgc agg tac ggc atg gtg atc acg ccc gac        240
Leu Tyr Cys Tyr Phe Gly Arg Arg Tyr Gly Met Val Ile Thr Pro Asp           
65                  70                  75                  80            

gcg gcc acg acc atc tcc gcc ggc atc gac ggc ggc cag ccc tgg cgg        288
Ala Ala Thr Thr Ile Ser Ala Gly Ile Asp Gly Gly Gln Pro Trp Arg           
                85                  90                  95                

cgg acc ttc ggc aac aac gtc acc tac acc gac tgg cgc cgc gac aac        336
Arg Thr Phe Gly Asn Asn Val Thr Tyr Thr Asp Trp Arg Arg Asp Asn           
            100                 105                 110                   

tac tac cag gcg gtg agg cag ctc ctg ccg ggt gtc agg cgg ctg ggc        384
Tyr Tyr Gln Ala Val Arg Gln Leu Leu Pro Gly Val Arg Arg Leu Gly           
        115                 120                 125                       

atc gaa ttc gac cac gtg tcg ctc gac ttc cgt cgg gac ctc gaa gcg        432
Ile Glu Phe Asp His Val Ser Leu Asp Phe Arg Arg Asp Leu Glu Ala           
    130                 135                 140                           

gcg ctg ccg ggg gtg gag ttc gtc gac gtg ggc cag ccc tcg atg tgg        480
Ala Leu Pro Gly Val Glu Phe Val Asp Val Gly Gln Pro Ser Met Trp           
145                 150                 155                 160           

atg cgc acc atc aag tcc gcg gag gag cag aag ctg atc cgc gaa ggc        528
Met Arg Thr Ile Lys Ser Ala Glu Glu Gln Lys Leu Ile Arg Glu Gly           
                165                 170                 175               

gcc cgc atc tgc gac atc ggc ggc gag gcg gtg gcg aag gcc gtg aag        576
Ala Arg Ile Cys Asp Ile Gly Gly Glu Ala Val Ala Lys Ala Val Lys           
            180                 185                 190                   

gcc ggc gtc ccg gag cac gag gtg gcc atc gcc tcg acc aac gcg atg        624
Ala Gly Val Pro Glu His Glu Val Ala Ile Ala Ser Thr Asn Ala Met           
        195                 200                 205                       

atc cgc gag atc gcc gag tcc ttt ccc tac gtc gag ctg atg gac acc        672
Ile Arg Glu Ile Ala Glu Ser Phe Pro Tyr Val Glu Leu Met Asp Thr           
    210                 215                 220                           

tgg acg tgg ttc cag tcc ggg atc aac acc gac ggc gcc cac aat ccg        720
Trp Thr Trp Phe Gln Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro           
225                 230                 235                 240           

gtc acg aac cgc gtg gtg cag tca ggc gac atc ctc tcg ctc aac tgc        768
Val Thr Asn Arg Val Val Gln Ser Gly Asp Ile Leu Ser Leu Asn Cys           
                245                 250                 255               

ttc ccc atg atc ttc ggc tat tac acc gcg ctc gag cgg acg atg ttc        816
Phe Pro Met Ile Phe Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Met Phe           
            260                 265                 270                   

tgc gac cac gtc gac gac gcg agc ctc gac gta tgg gag aag aac gtc        864
Cys Asp His Val Asp Asp Ala Ser Leu Asp Val Trp Glu Lys Asn Val           
        275                 280                 285                       

gcg gtt cac cgg cgc ggg ctc gag ctg atc cgc ccc ggg aag aag tgc        912
Ala Val His Arg Arg Gly Leu Glu Leu Ile Arg Pro Gly Lys Lys Cys           
    290                 295                 300                           

ggg gag atc gcg cag gag ctg aac cag atg tac cgc gag tgg gat ctg        960
Gly Glu Ile Ala Gln Glu Leu Asn Gln Met Tyr Arg Glu Trp Asp Leu           
305                 310                 315                 320           

ctg cag tac cgc tcc ttc ggc tac ggc cac tca ttc ggg gtc ctc tcc       1008
Leu Gln Tyr Arg Ser Phe Gly Tyr Gly His Ser Phe Gly Val Leu Ser           
                325                 330                 335               

cac tac tac gga cgc gaa gcc ggg gtg gaa ctg cgg gag gac atc gac       1056
His Tyr Tyr Gly Arg Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Asp           
            340                 345                 350                   

acc gag ctc aag ccc ggc atg gtc gtg tcc atg gag ccg atg gtg atg       1104
Thr Glu Leu Lys Pro Gly Met Val Val Ser Met Glu Pro Met Val Met           
        355                 360                 365                       

atc ccc gag ggg aag ccc ggc gcc ggt ggc tac cgg gag cac gac ata       1152
Ile Pro Glu Gly Lys Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile           
    370                 375                 380                           

ctg atc gtc acc gag gat ggc gcg gag aac ata acc ggc ttc ccg ttc       1200
Leu Ile Val Thr Glu Asp Gly Ala Glu Asn Ile Thr Gly Phe Pro Phe           
385                 390                 395                 400           

ggg ccg gag cac aac gtg atc cgc aac tga                               1230
Gly Pro Glu His Asn Val Ile Arg Asn                                       
                405                                                       


<210>  34
<211>  409
<212>  PRT
<213>  Lutibaculum baratangense

<400>  34

Met Leu Asp Lys Thr Ile Leu Asp Asp Met Val His Val Thr Glu Trp 
1               5                   10                  15      


His Asn Gly Glu Lys Glu Phe Leu Pro Phe Ser Asp Ala Glu Met Ser 
            20                  25                  30          


Arg Arg Gln Asp Asp Val Arg Ser Trp Met Gly Ala Asn Asn Val Asp 
        35                  40                  45              


Ala Ala Leu Phe Thr Ser Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp 
    50                  55                  60                  


Leu Tyr Cys Tyr Phe Gly Arg Arg Tyr Gly Met Val Ile Thr Pro Asp 
65                  70                  75                  80  


Ala Ala Thr Thr Ile Ser Ala Gly Ile Asp Gly Gly Gln Pro Trp Arg 
                85                  90                  95      


Arg Thr Phe Gly Asn Asn Val Thr Tyr Thr Asp Trp Arg Arg Asp Asn 
            100                 105                 110         


Tyr Tyr Gln Ala Val Arg Gln Leu Leu Pro Gly Val Arg Arg Leu Gly 
        115                 120                 125             


Ile Glu Phe Asp His Val Ser Leu Asp Phe Arg Arg Asp Leu Glu Ala 
    130                 135                 140                 


Ala Leu Pro Gly Val Glu Phe Val Asp Val Gly Gln Pro Ser Met Trp 
145                 150                 155                 160 


Met Arg Thr Ile Lys Ser Ala Glu Glu Gln Lys Leu Ile Arg Glu Gly 
                165                 170                 175     


Ala Arg Ile Cys Asp Ile Gly Gly Glu Ala Val Ala Lys Ala Val Lys 
            180                 185                 190         


Ala Gly Val Pro Glu His Glu Val Ala Ile Ala Ser Thr Asn Ala Met 
        195                 200                 205             


Ile Arg Glu Ile Ala Glu Ser Phe Pro Tyr Val Glu Leu Met Asp Thr 
    210                 215                 220                 


Trp Thr Trp Phe Gln Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro 
225                 230                 235                 240 


Val Thr Asn Arg Val Val Gln Ser Gly Asp Ile Leu Ser Leu Asn Cys 
                245                 250                 255     


Phe Pro Met Ile Phe Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Met Phe 
            260                 265                 270         


Cys Asp His Val Asp Asp Ala Ser Leu Asp Val Trp Glu Lys Asn Val 
        275                 280                 285             


Ala Val His Arg Arg Gly Leu Glu Leu Ile Arg Pro Gly Lys Lys Cys 
    290                 295                 300                 


Gly Glu Ile Ala Gln Glu Leu Asn Gln Met Tyr Arg Glu Trp Asp Leu 
305                 310                 315                 320 


Leu Gln Tyr Arg Ser Phe Gly Tyr Gly His Ser Phe Gly Val Leu Ser 
                325                 330                 335     


His Tyr Tyr Gly Arg Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Asp 
            340                 345                 350         


Thr Glu Leu Lys Pro Gly Met Val Val Ser Met Glu Pro Met Val Met 
        355                 360                 365             


Ile Pro Glu Gly Lys Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile 
    370                 375                 380                 


Leu Ile Val Thr Glu Asp Gly Ala Glu Asn Ile Thr Gly Phe Pro Phe 
385                 390                 395                 400 


Gly Pro Glu His Asn Val Ile Arg Asn 
                405                 


<210>  35
<211>  409
<212>  PRT
<213>  Lutibaculum baratangense


<220>
<221>  mat_peptide
<222>  (1)..(409)
<223>  Mutant creatine amidinohydrolase _Lutibaculum baratangense V4RGE5
       with C180A+C304A  substitutions

<400>  35

Met Leu Asp Lys Thr Ile Leu Asp Asp Met Val His Val Thr Glu Trp 
1               5                   10                  15      


His Asn Gly Glu Lys Glu Phe Leu Pro Phe Ser Asp Ala Glu Met Ser 
            20                  25                  30          


Arg Arg Gln Asp Asp Val Arg Ser Trp Met Gly Ala Asn Asn Val Asp 
        35                  40                  45              


Ala Ala Leu Phe Thr Ser Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp 
    50                  55                  60                  


Leu Tyr Cys Tyr Phe Gly Arg Arg Tyr Gly Met Val Ile Thr Pro Asp 
65                  70                  75                  80  


Ala Ala Thr Thr Ile Ser Ala Gly Ile Asp Gly Gly Gln Pro Trp Arg 
                85                  90                  95      


Arg Thr Phe Gly Asn Asn Val Thr Tyr Thr Asp Trp Arg Arg Asp Asn 
            100                 105                 110         


Tyr Tyr Gln Ala Val Arg Gln Leu Leu Pro Gly Val Arg Arg Leu Gly 
        115                 120                 125             


Ile Glu Phe Asp His Val Ser Leu Asp Phe Arg Arg Asp Leu Glu Ala 
    130                 135                 140                 


Ala Leu Pro Gly Val Glu Phe Val Asp Val Gly Gln Pro Ser Met Trp 
145                 150                 155                 160 


Met Arg Thr Ile Lys Ser Ala Glu Glu Gln Lys Leu Ile Arg Glu Gly 
                165                 170                 175     


Ala Arg Ile Ala Asp Ile Gly Gly Glu Ala Val Ala Lys Ala Val Lys 
            180                 185                 190         


Ala Gly Val Pro Glu His Glu Val Ala Ile Ala Ser Thr Asn Ala Met 
        195                 200                 205             


Ile Arg Glu Ile Ala Glu Ser Phe Pro Tyr Val Glu Leu Met Asp Thr 
    210                 215                 220                 


Trp Thr Trp Phe Gln Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro 
225                 230                 235                 240 


Val Thr Asn Arg Val Val Gln Ser Gly Asp Ile Leu Ser Leu Asn Cys 
                245                 250                 255     


Phe Pro Met Ile Phe Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Met Phe 
            260                 265                 270         


Cys Asp His Val Asp Asp Ala Ser Leu Asp Val Trp Glu Lys Asn Val 
        275                 280                 285             


Ala Val His Arg Arg Gly Leu Glu Leu Ile Arg Pro Gly Lys Lys Ala 
    290                 295                 300                 


Gly Glu Ile Ala Gln Glu Leu Asn Gln Met Tyr Arg Glu Trp Asp Leu 
305                 310                 315                 320 


Leu Gln Tyr Arg Ser Phe Gly Tyr Gly His Ser Phe Gly Val Leu Ser 
                325                 330                 335     


His Tyr Tyr Gly Arg Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Asp 
            340                 345                 350         


Thr Glu Leu Lys Pro Gly Met Val Val Ser Met Glu Pro Met Val Met 
        355                 360                 365             


Ile Pro Glu Gly Lys Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile 
    370                 375                 380                 


Leu Ile Val Thr Glu Asp Gly Ala Glu Asn Ile Thr Gly Phe Pro Phe 
385                 390                 395                 400 


Gly Pro Glu His Asn Val Ile Arg Asn 
                405                 


<210>  36
<211>  409
<212>  PRT
<213>  Lutibaculum baratangense


<220>
<221>  mat_peptide
<222>  (1)..(409)
<223>  Mutant creatine amidinohydrolase _Lutibaculum baratangense V4RGE5
       with C180A+C304A+C273L substitutions

<400>  36

Met Leu Asp Lys Thr Ile Leu Asp Asp Met Val His Val Thr Glu Trp 
1               5                   10                  15      


His Asn Gly Glu Lys Glu Phe Leu Pro Phe Ser Asp Ala Glu Met Ser 
            20                  25                  30          


Arg Arg Gln Asp Asp Val Arg Ser Trp Met Gly Ala Asn Asn Val Asp 
        35                  40                  45              


Ala Ala Leu Phe Thr Ser Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp 
    50                  55                  60                  


Leu Tyr Cys Tyr Phe Gly Arg Arg Tyr Gly Met Val Ile Thr Pro Asp 
65                  70                  75                  80  


Ala Ala Thr Thr Ile Ser Ala Gly Ile Asp Gly Gly Gln Pro Trp Arg 
                85                  90                  95      


Arg Thr Phe Gly Asn Asn Val Thr Tyr Thr Asp Trp Arg Arg Asp Asn 
            100                 105                 110         


Tyr Tyr Gln Ala Val Arg Gln Leu Leu Pro Gly Val Arg Arg Leu Gly 
        115                 120                 125             


Ile Glu Phe Asp His Val Ser Leu Asp Phe Arg Arg Asp Leu Glu Ala 
    130                 135                 140                 


Ala Leu Pro Gly Val Glu Phe Val Asp Val Gly Gln Pro Ser Met Trp 
145                 150                 155                 160 


Met Arg Thr Ile Lys Ser Ala Glu Glu Gln Lys Leu Ile Arg Glu Gly 
                165                 170                 175     


Ala Arg Ile Ala Asp Ile Gly Gly Glu Ala Val Ala Lys Ala Val Lys 
            180                 185                 190         


Ala Gly Val Pro Glu His Glu Val Ala Ile Ala Ser Thr Asn Ala Met 
        195                 200                 205             


Ile Arg Glu Ile Ala Glu Ser Phe Pro Tyr Val Glu Leu Met Asp Thr 
    210                 215                 220                 


Trp Thr Trp Phe Gln Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro 
225                 230                 235                 240 


Val Thr Asn Arg Val Val Gln Ser Gly Asp Ile Leu Ser Leu Asn Cys 
                245                 250                 255     


Phe Pro Met Ile Phe Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Met Phe 
            260                 265                 270         


Leu Asp His Val Asp Asp Ala Ser Leu Asp Val Trp Glu Lys Asn Val 
        275                 280                 285             


Ala Val His Arg Arg Gly Leu Glu Leu Ile Arg Pro Gly Lys Lys Ala 
    290                 295                 300                 


Gly Glu Ile Ala Gln Glu Leu Asn Gln Met Tyr Arg Glu Trp Asp Leu 
305                 310                 315                 320 


Leu Gln Tyr Arg Ser Phe Gly Tyr Gly His Ser Phe Gly Val Leu Ser 
                325                 330                 335     


His Tyr Tyr Gly Arg Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Asp 
            340                 345                 350         


Thr Glu Leu Lys Pro Gly Met Val Val Ser Met Glu Pro Met Val Met 
        355                 360                 365             


Ile Pro Glu Gly Lys Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile 
    370                 375                 380                 


Leu Ile Val Thr Glu Asp Gly Ala Glu Asn Ile Thr Gly Phe Pro Phe 
385                 390                 395                 400 


Gly Pro Glu His Asn Val Ile Arg Asn 
                405                 


<210>  37
<211>  1218
<212>  DNA
<213>  Roseobacter sp


<220>
<221>  CDS
<222>  (1)..(1218)
<223>  EDM71125.1 Roseobacter sp. AzwK-3b gene encoding creatine 
       amidinohydrolase [A6FQQ7]

<400>  37
atg ctt gac gac atg ctc cac gtg acc gaa tgg cac aat gga gaa aag         48
Met Leu Asp Asp Met Leu His Val Thr Glu Trp His Asn Gly Glu Lys           
1               5                   10                  15                

gaa ttt tcg cct ttt tcc gac aac gaa atg gcg cgc agg cag aat gaa         96
Glu Phe Ser Pro Phe Ser Asp Asn Glu Met Ala Arg Arg Gln Asn Glu           
            20                  25                  30                    

ctg cgc gac tgg atg gcc aag aac gac gtc gat gcg gtg ctg ctc acg        144
Leu Arg Asp Trp Met Ala Lys Asn Asp Val Asp Ala Val Leu Leu Thr           
        35                  40                  45                        

tct tat cac tgc att aca tat tac tca ggc tgg ctg tat tgc tat ttc        192
Ser Tyr His Cys Ile Thr Tyr Tyr Ser Gly Trp Leu Tyr Cys Tyr Phe           
    50                  55                  60                            

ggt cgc aaa tac gga atg gtg atc gac cag aag act gcc acc acc gtt        240
Gly Arg Lys Tyr Gly Met Val Ile Asp Gln Lys Thr Ala Thr Thr Val           
65                  70                  75                  80            

tcg gcg ggc atc gat ggc ggt caa ccc tgg cgc cgg agc ttt ggc aac        288
Ser Ala Gly Ile Asp Gly Gly Gln Pro Trp Arg Arg Ser Phe Gly Asn           
                85                  90                  95                

aac gtc acc tat acc gat tgg cgg cgc gac aat ttc tat cgt gca gtg        336
Asn Val Thr Tyr Thr Asp Trp Arg Arg Asp Asn Phe Tyr Arg Ala Val           
            100                 105                 110                   

cag ggg ctt acc cag ggc gcg cgg cgt gtg ggc atc gag ttc gac cat        384
Gln Gly Leu Thr Gln Gly Ala Arg Arg Val Gly Ile Glu Phe Asp His           
        115                 120                 125                       

gtc tcg ctg gaa tat cgc gat ctg ttg cag gat gcg ctg ccg ggt gtg        432
Val Ser Leu Glu Tyr Arg Asp Leu Leu Gln Asp Ala Leu Pro Gly Val           
    130                 135                 140                           

gac ttc gtc gat gtc agc cag ccg tcg atg tgg atg cgc acg atc aaa        480
Asp Phe Val Asp Val Ser Gln Pro Ser Met Trp Met Arg Thr Ile Lys           
145                 150                 155                 160           

tca gac gag gag atc aag ctg atc cgc gaa ggc gca cgg gtg gcg gat        528
Ser Asp Glu Glu Ile Lys Leu Ile Arg Glu Gly Ala Arg Val Ala Asp           
                165                 170                 175               

gtc ggc ggt tat gcc gtg gcc gcc gcc gtg caa gcg ggc gtg ccg gag        576
Val Gly Gly Tyr Ala Val Ala Ala Ala Val Gln Ala Gly Val Pro Glu           
            180                 185                 190                   

cat gag gtc gcc atc gcg ggc acc aat gcg atg atc cgc gag atc gcc        624
His Glu Val Ala Ile Ala Gly Thr Asn Ala Met Ile Arg Glu Ile Ala           
        195                 200                 205                       

aaa tcc ttc ccc ttt gtc gag ttg atg gac acc tgg acc tgg ttc cag        672
Lys Ser Phe Pro Phe Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln           
    210                 215                 220                           

tca ggt atc aat acc gac ggt gcg cat aac ccg gtg acg aac cgc gtg        720
Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro Val Thr Asn Arg Val           
225                 230                 235                 240           

gtg caa tcg ggc gat atc ctc agc ctc aac acc ttt ccg atg atc ttc        768
Val Gln Ser Gly Asp Ile Leu Ser Leu Asn Thr Phe Pro Met Ile Phe           
                245                 250                 255               

ggc tat tac acc gcg ctg gag cgc acg ctg ttt tgc gac tcg gtg gat        816
Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Leu Phe Cys Asp Ser Val Asp           
            260                 265                 270                   

gat gcg agc ttg gat gtc tgg gaa aag aac gtg gca gtg cat cgt cgc        864
Asp Ala Ser Leu Asp Val Trp Glu Lys Asn Val Ala Val His Arg Arg           
        275                 280                 285                       

gga ctc gag ctt atg aag ccg ggt gcg cgc tgc atg gat atc gcg atc        912
Gly Leu Glu Leu Met Lys Pro Gly Ala Arg Cys Met Asp Ile Ala Ile           
    290                 295                 300                           

gaa ctg aac gag atg tat cgc gaa tgg gac ctg ctg aaa tac cgc tcg        960
Glu Leu Asn Glu Met Tyr Arg Glu Trp Asp Leu Leu Lys Tyr Arg Ser           
305                 310                 315                 320           

ttc ggc tat ggc cac agc ttt ggc gtg ctc agt cac tat tac ggg cgc       1008
Phe Gly Tyr Gly His Ser Phe Gly Val Leu Ser His Tyr Tyr Gly Arg           
                325                 330                 335               

gag gcc ggg gtc gaa ctg cgc gag gat atc gac acg gtg ctc aag ccc       1056
Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Asp Thr Val Leu Lys Pro           
            340                 345                 350                   

ggc atg gtc gtg tcc atg gag ccg atg gtc atg atc ccc gaa ggg gcg       1104
Gly Met Val Val Ser Met Glu Pro Met Val Met Ile Pro Glu Gly Ala           
        355                 360                 365                       

ccc ggt gcg ggc ggc tac cgc gag cat gat atc ctc gtg atc ggc gag       1152
Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile Leu Val Ile Gly Glu           
    370                 375                 380                           

gat ggg gcg gag aac atc acc ggg ttt ccc ttc ggc ccc gag cac aac       1200
Asp Gly Ala Glu Asn Ile Thr Gly Phe Pro Phe Gly Pro Glu His Asn           
385                 390                 395                 400           

atc gtc ggc tcc aac tga                                               1218
Ile Val Gly Ser Asn                                                       
                405                                                       


<210>  38
<211>  405
<212>  PRT
<213>  Roseobacter sp

<400>  38

Met Leu Asp Asp Met Leu His Val Thr Glu Trp His Asn Gly Glu Lys 
1               5                   10                  15      


Glu Phe Ser Pro Phe Ser Asp Asn Glu Met Ala Arg Arg Gln Asn Glu 
            20                  25                  30          


Leu Arg Asp Trp Met Ala Lys Asn Asp Val Asp Ala Val Leu Leu Thr 
        35                  40                  45              


Ser Tyr His Cys Ile Thr Tyr Tyr Ser Gly Trp Leu Tyr Cys Tyr Phe 
    50                  55                  60                  


Gly Arg Lys Tyr Gly Met Val Ile Asp Gln Lys Thr Ala Thr Thr Val 
65                  70                  75                  80  


Ser Ala Gly Ile Asp Gly Gly Gln Pro Trp Arg Arg Ser Phe Gly Asn 
                85                  90                  95      


Asn Val Thr Tyr Thr Asp Trp Arg Arg Asp Asn Phe Tyr Arg Ala Val 
            100                 105                 110         


Gln Gly Leu Thr Gln Gly Ala Arg Arg Val Gly Ile Glu Phe Asp His 
        115                 120                 125             


Val Ser Leu Glu Tyr Arg Asp Leu Leu Gln Asp Ala Leu Pro Gly Val 
    130                 135                 140                 


Asp Phe Val Asp Val Ser Gln Pro Ser Met Trp Met Arg Thr Ile Lys 
145                 150                 155                 160 


Ser Asp Glu Glu Ile Lys Leu Ile Arg Glu Gly Ala Arg Val Ala Asp 
                165                 170                 175     


Val Gly Gly Tyr Ala Val Ala Ala Ala Val Gln Ala Gly Val Pro Glu 
            180                 185                 190         


His Glu Val Ala Ile Ala Gly Thr Asn Ala Met Ile Arg Glu Ile Ala 
        195                 200                 205             


Lys Ser Phe Pro Phe Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln 
    210                 215                 220                 


Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro Val Thr Asn Arg Val 
225                 230                 235                 240 


Val Gln Ser Gly Asp Ile Leu Ser Leu Asn Thr Phe Pro Met Ile Phe 
                245                 250                 255     


Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Leu Phe Cys Asp Ser Val Asp 
            260                 265                 270         


Asp Ala Ser Leu Asp Val Trp Glu Lys Asn Val Ala Val His Arg Arg 
        275                 280                 285             


Gly Leu Glu Leu Met Lys Pro Gly Ala Arg Cys Met Asp Ile Ala Ile 
    290                 295                 300                 


Glu Leu Asn Glu Met Tyr Arg Glu Trp Asp Leu Leu Lys Tyr Arg Ser 
305                 310                 315                 320 


Phe Gly Tyr Gly His Ser Phe Gly Val Leu Ser His Tyr Tyr Gly Arg 
                325                 330                 335     


Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Asp Thr Val Leu Lys Pro 
            340                 345                 350         


Gly Met Val Val Ser Met Glu Pro Met Val Met Ile Pro Glu Gly Ala 
        355                 360                 365             


Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile Leu Val Ile Gly Glu 
    370                 375                 380                 


Asp Gly Ala Glu Asn Ile Thr Gly Phe Pro Phe Gly Pro Glu His Asn 
385                 390                 395                 400 


Ile Val Gly Ser Asn 
                405 


<210>  39
<211>  405
<212>  PRT
<213>  Roseobacter sp


<220>
<221>  mat_peptide
<222>  (1)..(405)
<223>  Mutant creatine amidinohydrolase Roseobacter sp. _A6FQQ7 with 
       C299A  substitution

<400>  39

Met Leu Asp Asp Met Leu His Val Thr Glu Trp His Asn Gly Glu Lys 
1               5                   10                  15      


Glu Phe Ser Pro Phe Ser Asp Asn Glu Met Ala Arg Arg Gln Asn Glu 
            20                  25                  30          


Leu Arg Asp Trp Met Ala Lys Asn Asp Val Asp Ala Val Leu Leu Thr 
        35                  40                  45              


Ser Tyr His Cys Ile Thr Tyr Tyr Ser Gly Trp Leu Tyr Cys Tyr Phe 
    50                  55                  60                  


Gly Arg Lys Tyr Gly Met Val Ile Asp Gln Lys Thr Ala Thr Thr Val 
65                  70                  75                  80  


Ser Ala Gly Ile Asp Gly Gly Gln Pro Trp Arg Arg Ser Phe Gly Asn 
                85                  90                  95      


Asn Val Thr Tyr Thr Asp Trp Arg Arg Asp Asn Phe Tyr Arg Ala Val 
            100                 105                 110         


Gln Gly Leu Thr Gln Gly Ala Arg Arg Val Gly Ile Glu Phe Asp His 
        115                 120                 125             


Val Ser Leu Glu Tyr Arg Asp Leu Leu Gln Asp Ala Leu Pro Gly Val 
    130                 135                 140                 


Asp Phe Val Asp Val Ser Gln Pro Ser Met Trp Met Arg Thr Ile Lys 
145                 150                 155                 160 


Ser Asp Glu Glu Ile Lys Leu Ile Arg Glu Gly Ala Arg Val Ala Asp 
                165                 170                 175     


Val Gly Gly Tyr Ala Val Ala Ala Ala Val Gln Ala Gly Val Pro Glu 
            180                 185                 190         


His Glu Val Ala Ile Ala Gly Thr Asn Ala Met Ile Arg Glu Ile Ala 
        195                 200                 205             


Lys Ser Phe Pro Phe Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln 
    210                 215                 220                 


Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro Val Thr Asn Arg Val 
225                 230                 235                 240 


Val Gln Ser Gly Asp Ile Leu Ser Leu Asn Thr Phe Pro Met Ile Phe 
                245                 250                 255     


Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Leu Phe Cys Asp Ser Val Asp 
            260                 265                 270         


Asp Ala Ser Leu Asp Val Trp Glu Lys Asn Val Ala Val His Arg Arg 
        275                 280                 285             


Gly Leu Glu Leu Met Lys Pro Gly Ala Arg Ala Met Asp Ile Ala Ile 
    290                 295                 300                 


Glu Leu Asn Glu Met Tyr Arg Glu Trp Asp Leu Leu Lys Tyr Arg Ser 
305                 310                 315                 320 


Phe Gly Tyr Gly His Ser Phe Gly Val Leu Ser His Tyr Tyr Gly Arg 
                325                 330                 335     


Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Asp Thr Val Leu Lys Pro 
            340                 345                 350         


Gly Met Val Val Ser Met Glu Pro Met Val Met Ile Pro Glu Gly Ala 
        355                 360                 365             


Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile Leu Val Ile Gly Glu 
    370                 375                 380                 


Asp Gly Ala Glu Asn Ile Thr Gly Phe Pro Phe Gly Pro Glu His Asn 
385                 390                 395                 400 


Ile Val Gly Ser Asn 
                405 


<210>  40
<211>  405
<212>  PRT
<213>  Roseobacter sp.


<220>
<221>  mat_peptide
<222>  (1)..(405)
<223>  Mutant creatine amidinohydrolase Roseobacter sp. _A6FQQ7 with 
       C299A+ C268L  substitution

<400>  40

Met Leu Asp Asp Met Leu His Val Thr Glu Trp His Asn Gly Glu Lys 
1               5                   10                  15      


Glu Phe Ser Pro Phe Ser Asp Asn Glu Met Ala Arg Arg Gln Asn Glu 
            20                  25                  30          


Leu Arg Asp Trp Met Ala Lys Asn Asp Val Asp Ala Val Leu Leu Thr 
        35                  40                  45              


Ser Tyr His Cys Ile Thr Tyr Tyr Ser Gly Trp Leu Tyr Cys Tyr Phe 
    50                  55                  60                  


Gly Arg Lys Tyr Gly Met Val Ile Asp Gln Lys Thr Ala Thr Thr Val 
65                  70                  75                  80  


Ser Ala Gly Ile Asp Gly Gly Gln Pro Trp Arg Arg Ser Phe Gly Asn 
                85                  90                  95      


Asn Val Thr Tyr Thr Asp Trp Arg Arg Asp Asn Phe Tyr Arg Ala Val 
            100                 105                 110         


Gln Gly Leu Thr Gln Gly Ala Arg Arg Val Gly Ile Glu Phe Asp His 
        115                 120                 125             


Val Ser Leu Glu Tyr Arg Asp Leu Leu Gln Asp Ala Leu Pro Gly Val 
    130                 135                 140                 


Asp Phe Val Asp Val Ser Gln Pro Ser Met Trp Met Arg Thr Ile Lys 
145                 150                 155                 160 


Ser Asp Glu Glu Ile Lys Leu Ile Arg Glu Gly Ala Arg Val Ala Asp 
                165                 170                 175     


Val Gly Gly Tyr Ala Val Ala Ala Ala Val Gln Ala Gly Val Pro Glu 
            180                 185                 190         


His Glu Val Ala Ile Ala Gly Thr Asn Ala Met Ile Arg Glu Ile Ala 
        195                 200                 205             


Lys Ser Phe Pro Phe Val Glu Leu Met Asp Thr Trp Thr Trp Phe Gln 
    210                 215                 220                 


Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro Val Thr Asn Arg Val 
225                 230                 235                 240 


Val Gln Ser Gly Asp Ile Leu Ser Leu Asn Thr Phe Pro Met Ile Phe 
                245                 250                 255     


Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Leu Phe Leu Asp Ser Val Asp 
            260                 265                 270         


Asp Ala Ser Leu Asp Val Trp Glu Lys Asn Val Ala Val His Arg Arg 
        275                 280                 285             


Gly Leu Glu Leu Met Lys Pro Gly Ala Arg Ala Met Asp Ile Ala Ile 
    290                 295                 300                 


Glu Leu Asn Glu Met Tyr Arg Glu Trp Asp Leu Leu Lys Tyr Arg Ser 
305                 310                 315                 320 


Phe Gly Tyr Gly His Ser Phe Gly Val Leu Ser His Tyr Tyr Gly Arg 
                325                 330                 335     


Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Asp Thr Val Leu Lys Pro 
            340                 345                 350         


Gly Met Val Val Ser Met Glu Pro Met Val Met Ile Pro Glu Gly Ala 
        355                 360                 365             


Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile Leu Val Ile Gly Glu 
    370                 375                 380                 


Asp Gly Ala Glu Asn Ile Thr Gly Phe Pro Phe Gly Pro Glu His Asn 
385                 390                 395                 400 


Ile Val Gly Ser Asn 
                405 


<210>  41
<211>  1233
<212>  DNA
<213>  Dinoroseobacter shibae


<220>
<221>  CDS
<222>  (1)..(1233)
<223>  ABV93862.1 Dinoroseobacter shibae DSM 16493 gene encoding 
       creatine amidinohydrolase [A8LQJ5]

<400>  41
atg gac ggt aat acc aac gtc gac gac atg ctc cac gtc atg gaa tgg         48
Met Asp Gly Asn Thr Asn Val Asp Asp Met Leu His Val Met Glu Trp           
1               5                   10                  15                

cat aac ggc gaa aaa gag ttc tcg ccc ttt tcg gac acc gag atg gcc         96
His Asn Gly Glu Lys Glu Phe Ser Pro Phe Ser Asp Thr Glu Met Ala           
            20                  25                  30                    

cgt cgg caa aac gaa ttg cgc gac tgg atg gcc aag aac gat gtc gat        144
Arg Arg Gln Asn Glu Leu Arg Asp Trp Met Ala Lys Asn Asp Val Asp           
        35                  40                  45                        

gcg tcg ctc ttc acc tcg tat cac tgc atc aac tat tac agc ggc tgg        192
Ala Ser Leu Phe Thr Ser Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp           
    50                  55                  60                            

ctg tat tgc tat ttc ggc cgt aaa tac ggc atg gtc atc gac cag aag        240
Leu Tyr Cys Tyr Phe Gly Arg Lys Tyr Gly Met Val Ile Asp Gln Lys           
65                  70                  75                  80            

aac gcc acg acg atc tcc gcc ggg atc gat ggc ggc cag ccc ttc cgc        288
Asn Ala Thr Thr Ile Ser Ala Gly Ile Asp Gly Gly Gln Pro Phe Arg           
                85                  90                  95                

cgg agc ttt ggc aac aac atc acc tac acc gac tgg cgc cgc gac aac        336
Arg Ser Phe Gly Asn Asn Ile Thr Tyr Thr Asp Trp Arg Arg Asp Asn           
            100                 105                 110                   

ttt tat cgc gcg atc cag cag ctg acc ccg ggc gcc aag cgc atc ggc        384
Phe Tyr Arg Ala Ile Gln Gln Leu Thr Pro Gly Ala Lys Arg Ile Gly           
        115                 120                 125                       

atc gag ttc gac cat gtc tcg ctc gag tac cgc cag ctg ctg cag gat        432
Ile Glu Phe Asp His Val Ser Leu Glu Tyr Arg Gln Leu Leu Gln Asp           
    130                 135                 140                           

gcg ctg ccg ggc gtc gag ttc gtc gat gtc ggc cag ccc gcc atg tgg        480
Ala Leu Pro Gly Val Glu Phe Val Asp Val Gly Gln Pro Ala Met Trp           
145                 150                 155                 160           

atg cgc acc atc aag tcc gcc gaa gag atc aag ctg atc aag gaa ggc        528
Met Arg Thr Ile Lys Ser Ala Glu Glu Ile Lys Leu Ile Lys Glu Gly           
                165                 170                 175               

gcg cgc gtc gcc gac gtg ggt ggc gcg gcc gtg gcc gca gcg gtc aag        576
Ala Arg Val Ala Asp Val Gly Gly Ala Ala Val Ala Ala Ala Val Lys           
            180                 185                 190                   

gcc ggt gtc ccc gag cat gaa gtg gcc atc gcc ggc acc acc gcg atg        624
Ala Gly Val Pro Glu His Glu Val Ala Ile Ala Gly Thr Thr Ala Met           
        195                 200                 205                       

atc cgc gag atc gcg aac tcc ttc ccc ttc gtc gag ctg atg gac acc        672
Ile Arg Glu Ile Ala Asn Ser Phe Pro Phe Val Glu Leu Met Asp Thr           
    210                 215                 220                           

tgg acc tgg ttc cag tcc ggc atc aac acc gac ggc gcc cat aac ccg        720
Trp Thr Trp Phe Gln Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro           
225                 230                 235                 240           

gtg acc aac aag aag gtg cag tcg ggc gag atc ctc agc ctc aac acc        768
Val Thr Asn Lys Lys Val Gln Ser Gly Glu Ile Leu Ser Leu Asn Thr           
                245                 250                 255               

ttc ccg atg atc ttc ggc tat tac acc gcg ctg gaa cgg acc ctg ttc        816
Phe Pro Met Ile Phe Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Leu Phe           
            260                 265                 270                   

tgt gac cat gtg gac gat gcc agc ctc gac atc tgg gaa aag aac gtg        864
Cys Asp His Val Asp Asp Ala Ser Leu Asp Ile Trp Glu Lys Asn Val           
        275                 280                 285                       

aag gtg cac gag cgt ggc ctg cag ctg atc aag ccc ggc gcg cgc tgc        912
Lys Val His Glu Arg Gly Leu Gln Leu Ile Lys Pro Gly Ala Arg Cys           
    290                 295                 300                           

atg gac atc gcg atc gag ctc aac gag atg tat cgc gag tgg gac ctg        960
Met Asp Ile Ala Ile Glu Leu Asn Glu Met Tyr Arg Glu Trp Asp Leu           
305                 310                 315                 320           

ctg aag tat cgc tcc ttc ggc tac ggg cac agc ttc ggt gtg ctg agc       1008
Leu Lys Tyr Arg Ser Phe Gly Tyr Gly His Ser Phe Gly Val Leu Ser           
                325                 330                 335               

cac tac tac ggc cgc gag gcg ggc gtc gag ctc cgc gag gat atc gaa       1056
His Tyr Tyr Gly Arg Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Glu           
            340                 345                 350                   

acc gag ctg aag cca ggg atg gtg gtg tcc atg gag ccg atg gtg atg       1104
Thr Glu Leu Lys Pro Gly Met Val Val Ser Met Glu Pro Met Val Met           
        355                 360                 365                       

atc ccc gag ggt cag ccg ggg gcc ggg ggc tac cgc gag cat gac atc       1152
Ile Pro Glu Gly Gln Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile           
    370                 375                 380                           

ctg gtc atc aac gac gac aac acg gtg gaa aac atc acc ggg ttc ccc       1200
Leu Val Ile Asn Asp Asp Asn Thr Val Glu Asn Ile Thr Gly Phe Pro           
385                 390                 395                 400           

ttc ggc ccc gag cac aac atc atc aag aac tga                           1233
Phe Gly Pro Glu His Asn Ile Ile Lys Asn                                   
                405                 410                                   


<210>  42
<211>  410
<212>  PRT
<213>  Dinoroseobacter shibae

<400>  42

Met Asp Gly Asn Thr Asn Val Asp Asp Met Leu His Val Met Glu Trp 
1               5                   10                  15      


His Asn Gly Glu Lys Glu Phe Ser Pro Phe Ser Asp Thr Glu Met Ala 
            20                  25                  30          


Arg Arg Gln Asn Glu Leu Arg Asp Trp Met Ala Lys Asn Asp Val Asp 
        35                  40                  45              


Ala Ser Leu Phe Thr Ser Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp 
    50                  55                  60                  


Leu Tyr Cys Tyr Phe Gly Arg Lys Tyr Gly Met Val Ile Asp Gln Lys 
65                  70                  75                  80  


Asn Ala Thr Thr Ile Ser Ala Gly Ile Asp Gly Gly Gln Pro Phe Arg 
                85                  90                  95      


Arg Ser Phe Gly Asn Asn Ile Thr Tyr Thr Asp Trp Arg Arg Asp Asn 
            100                 105                 110         


Phe Tyr Arg Ala Ile Gln Gln Leu Thr Pro Gly Ala Lys Arg Ile Gly 
        115                 120                 125             


Ile Glu Phe Asp His Val Ser Leu Glu Tyr Arg Gln Leu Leu Gln Asp 
    130                 135                 140                 


Ala Leu Pro Gly Val Glu Phe Val Asp Val Gly Gln Pro Ala Met Trp 
145                 150                 155                 160 


Met Arg Thr Ile Lys Ser Ala Glu Glu Ile Lys Leu Ile Lys Glu Gly 
                165                 170                 175     


Ala Arg Val Ala Asp Val Gly Gly Ala Ala Val Ala Ala Ala Val Lys 
            180                 185                 190         


Ala Gly Val Pro Glu His Glu Val Ala Ile Ala Gly Thr Thr Ala Met 
        195                 200                 205             


Ile Arg Glu Ile Ala Asn Ser Phe Pro Phe Val Glu Leu Met Asp Thr 
    210                 215                 220                 


Trp Thr Trp Phe Gln Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro 
225                 230                 235                 240 


Val Thr Asn Lys Lys Val Gln Ser Gly Glu Ile Leu Ser Leu Asn Thr 
                245                 250                 255     


Phe Pro Met Ile Phe Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Leu Phe 
            260                 265                 270         


Cys Asp His Val Asp Asp Ala Ser Leu Asp Ile Trp Glu Lys Asn Val 
        275                 280                 285             


Lys Val His Glu Arg Gly Leu Gln Leu Ile Lys Pro Gly Ala Arg Cys 
    290                 295                 300                 


Met Asp Ile Ala Ile Glu Leu Asn Glu Met Tyr Arg Glu Trp Asp Leu 
305                 310                 315                 320 


Leu Lys Tyr Arg Ser Phe Gly Tyr Gly His Ser Phe Gly Val Leu Ser 
                325                 330                 335     


His Tyr Tyr Gly Arg Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Glu 
            340                 345                 350         


Thr Glu Leu Lys Pro Gly Met Val Val Ser Met Glu Pro Met Val Met 
        355                 360                 365             


Ile Pro Glu Gly Gln Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile 
    370                 375                 380                 


Leu Val Ile Asn Asp Asp Asn Thr Val Glu Asn Ile Thr Gly Phe Pro 
385                 390                 395                 400 


Phe Gly Pro Glu His Asn Ile Ile Lys Asn 
                405                 410 


<210>  43
<211>  410
<212>  PRT
<213>  Dinoroseobacter shibae


<220>
<221>  mat_peptide
<222>  (1)..(410)
<223>  Mutant creatine amidinohydrolase Roseobacter sp. _A8LQJ5 with 
       C304A  substitution

<400>  43

Met Asp Gly Asn Thr Asn Val Asp Asp Met Leu His Val Met Glu Trp 
1               5                   10                  15      


His Asn Gly Glu Lys Glu Phe Ser Pro Phe Ser Asp Thr Glu Met Ala 
            20                  25                  30          


Arg Arg Gln Asn Glu Leu Arg Asp Trp Met Ala Lys Asn Asp Val Asp 
        35                  40                  45              


Ala Ser Leu Phe Thr Ser Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp 
    50                  55                  60                  


Leu Tyr Cys Tyr Phe Gly Arg Lys Tyr Gly Met Val Ile Asp Gln Lys 
65                  70                  75                  80  


Asn Ala Thr Thr Ile Ser Ala Gly Ile Asp Gly Gly Gln Pro Phe Arg 
                85                  90                  95      


Arg Ser Phe Gly Asn Asn Ile Thr Tyr Thr Asp Trp Arg Arg Asp Asn 
            100                 105                 110         


Phe Tyr Arg Ala Ile Gln Gln Leu Thr Pro Gly Ala Lys Arg Ile Gly 
        115                 120                 125             


Ile Glu Phe Asp His Val Ser Leu Glu Tyr Arg Gln Leu Leu Gln Asp 
    130                 135                 140                 


Ala Leu Pro Gly Val Glu Phe Val Asp Val Gly Gln Pro Ala Met Trp 
145                 150                 155                 160 


Met Arg Thr Ile Lys Ser Ala Glu Glu Ile Lys Leu Ile Lys Glu Gly 
                165                 170                 175     


Ala Arg Val Ala Asp Val Gly Gly Ala Ala Val Ala Ala Ala Val Lys 
            180                 185                 190         


Ala Gly Val Pro Glu His Glu Val Ala Ile Ala Gly Thr Thr Ala Met 
        195                 200                 205             


Ile Arg Glu Ile Ala Asn Ser Phe Pro Phe Val Glu Leu Met Asp Thr 
    210                 215                 220                 


Trp Thr Trp Phe Gln Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro 
225                 230                 235                 240 


Val Thr Asn Lys Lys Val Gln Ser Gly Glu Ile Leu Ser Leu Asn Thr 
                245                 250                 255     


Phe Pro Met Ile Phe Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Leu Phe 
            260                 265                 270         


Cys Asp His Val Asp Asp Ala Ser Leu Asp Ile Trp Glu Lys Asn Val 
        275                 280                 285             


Lys Val His Glu Arg Gly Leu Gln Leu Ile Lys Pro Gly Ala Arg Ala 
    290                 295                 300                 


Met Asp Ile Ala Ile Glu Leu Asn Glu Met Tyr Arg Glu Trp Asp Leu 
305                 310                 315                 320 


Leu Lys Tyr Arg Ser Phe Gly Tyr Gly His Ser Phe Gly Val Leu Ser 
                325                 330                 335     


His Tyr Tyr Gly Arg Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Glu 
            340                 345                 350         


Thr Glu Leu Lys Pro Gly Met Val Val Ser Met Glu Pro Met Val Met 
        355                 360                 365             


Ile Pro Glu Gly Gln Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile 
    370                 375                 380                 


Leu Val Ile Asn Asp Asp Asn Thr Val Glu Asn Ile Thr Gly Phe Pro 
385                 390                 395                 400 


Phe Gly Pro Glu His Asn Ile Ile Lys Asn 
                405                 410 


<210>  44
<211>  409
<212>  PRT
<213>  Dinoroseobacter shibae


<220>
<221>  mat_peptide
<222>  (1)..(409)
<223>  Mutant creatine amidinohydrolase Roseobacter sp._A8LQJ5 with 
       C304A +C273L  substitutions

<400>  44

Met Asp Gly Asn Thr Asn Val Asp Asp Met Leu His Val Met Glu Trp 
1               5                   10                  15      


His Asn Gly Glu Lys Glu Phe Ser Pro Phe Ser Asp Thr Glu Met Ala 
            20                  25                  30          


Arg Arg Gln Asn Glu Leu Arg Asp Trp Met Ala Lys Asn Asp Val Asp 
        35                  40                  45              


Ala Ser Leu Phe Thr Ser Tyr His Cys Ile Asn Tyr Tyr Ser Gly Trp 
    50                  55                  60                  


Leu Tyr Cys Tyr Phe Gly Arg Lys Tyr Gly Met Val Ile Asp Gln Lys 
65                  70                  75                  80  


Asn Ala Thr Thr Ile Ser Ala Gly Ile Asp Gly Gly Gln Pro Phe Arg 
                85                  90                  95      


Arg Ser Phe Gly Asn Asn Ile Thr Tyr Thr Asp Trp Arg Arg Asp Asn 
            100                 105                 110         


Phe Tyr Arg Ala Ile Gln Gln Leu Thr Pro Gly Ala Lys Arg Ile Gly 
        115                 120                 125             


Ile Glu Phe Asp His Val Ser Leu Glu Tyr Arg Gln Leu Leu Gln Asp 
    130                 135                 140                 


Ala Leu Pro Gly Val Glu Phe Val Asp Val Gly Gln Pro Ala Met Trp 
145                 150                 155                 160 


Met Arg Thr Ile Lys Ser Ala Glu Glu Ile Lys Leu Ile Lys Glu Gly 
                165                 170                 175     


Ala Arg Val Ala Asp Val Gly Gly Ala Ala Val Ala Ala Ala Val Lys 
            180                 185                 190         


Ala Gly Val Pro Glu His Glu Val Ala Ile Ala Gly Thr Thr Ala Met 
        195                 200                 205             


Ile Arg Glu Ile Ala Asn Ser Phe Pro Phe Val Glu Leu Met Asp Thr 
    210                 215                 220                 


Trp Thr Trp Phe Gln Ser Gly Ile Asn Thr Asp Gly Ala His Asn Pro 
225                 230                 235                 240 


Val Thr Asn Lys Lys Val Gln Ser Gly Glu Ile Leu Ser Leu Asn Thr 
                245                 250                 255     


Phe Pro Met Ile Phe Gly Tyr Tyr Thr Ala Leu Glu Arg Thr Leu Phe 
            260                 265                 270         


Leu Asp His Val Asp Asp Ala Ser Leu Asp Ile Trp Glu Lys Asn Val 
        275                 280                 285             


Lys Val His Glu Arg Gly Leu Gln Leu Ile Lys Pro Gly Ala Arg Ala 
    290                 295                 300                 


Met Asp Ile Ala Ile Glu Leu Asn Glu Met Tyr Arg Glu Trp Asp Leu 
305                 310                 315                 320 


Leu Lys Tyr Arg Ser Phe Gly Tyr Gly His Ser Phe Gly Val Leu Ser 
                325                 330                 335     


His Tyr Tyr Gly Arg Glu Ala Gly Val Glu Leu Arg Glu Asp Ile Glu 
            340                 345                 350         


Thr Glu Leu Lys Pro Gly Met Val Val Ser Met Glu Pro Met Val Met 
        355                 360                 365             


Ile Pro Glu Gly Gln Pro Gly Ala Gly Gly Tyr Arg Glu His Asp Ile 
    370                 375                 380                 


Leu Val Ile Asn Asp Asp Asn Thr Val Glu Asn Ile Thr Gly Phe Pro 
385                 390                 395                 400 


Phe Gly Pro Glu His Asn Ile Ile Lys 
                405                 


<210>  45
<211>  1140
<212>  DNA
<213>  Paracoccus denitrificans


<220>
<221>  CDS
<222>  (1)..(1140)
<223>  ABL71480.1 Paracoccus denitrificans PD1222 gene encoding creatine
       amidinohydrolase [A1B7I6]

<400>  45
atg cag cgg cgc cag gac gac atg cgc cgc tgg atg gcc ggg aac ggc         48
Met Gln Arg Arg Gln Asp Asp Met Arg Arg Trp Met Ala Gly Asn Gly           
1               5                   10                  15                

gtc gat gcg gca ctg ttc acc tcg tat cac tgc atc aac tat tat tcg         96
Val Asp Ala Ala Leu Phe Thr Ser Tyr His Cys Ile Asn Tyr Tyr Ser           
            20                  25                  30                    

ggc tgg ctc tac tgc tat ttc ggc cgc aaa tac ggc atg gtc atc acc        144
Gly Trp Leu Tyr Cys Tyr Phe Gly Arg Lys Tyr Gly Met Val Ile Thr           
        35                  40                  45                        

cag gac gcg gcg acc acc atc agc gcc ggc atc gat ggc ggt cag ccg        192
Gln Asp Ala Ala Thr Thr Ile Ser Ala Gly Ile Asp Gly Gly Gln Pro           
    50                  55                  60                            

tgg cgg cgc agc ttt ggc ggc aac gtc acc tat acc gat tgg cgg cgc        240
Trp Arg Arg Ser Phe Gly Gly Asn Val Thr Tyr Thr Asp Trp Arg Arg           
65                  70                  75                  80            

gac aat tat ttc cgc gcg gtg cgg cag ctg acc ccc ggc gtc aag cgg        288
Asp Asn Tyr Phe Arg Ala Val Arg Gln Leu Thr Pro Gly Val Lys Arg           
                85                  90                  95                

ctg gga atc gag ttc gac cat gtc aac atg gac ttg cgc cgc cag ctt        336
Leu Gly Ile Glu Phe Asp His Val Asn Met Asp Leu Arg Arg Gln Leu           
            100                 105                 110                   

gag gca gcc ctg ccg ggg gtg gaa ttc gtc gat gtc ggc cag ccc tcg        384
Glu Ala Ala Leu Pro Gly Val Glu Phe Val Asp Val Gly Gln Pro Ser           
        115                 120                 125                       

atg tgg atg cgc tcg atc aag tcg gcc gag gaa cac aag ctg atc cgc        432
Met Trp Met Arg Ser Ile Lys Ser Ala Glu Glu His Lys Leu Ile Arg           
    130                 135                 140                           

gag ggc gcg cgc atc tgc gac gtg ggc ggc gcg gcg gtg gcg gct gcg        480
Glu Gly Ala Arg Ile Cys Asp Val Gly Gly Ala Ala Val Ala Ala Ala           
145                 150                 155                 160           

gtc aag gcg ggc gtg ccc gag cac gag gtc gcc atc gcc tcg acc aat        528
Val Lys Ala Gly Val Pro Glu His Glu Val Ala Ile Ala Ser Thr Asn           
                165                 170                 175               

gcc atg atc cgc gag gtc gcc gcc tcc ttc ccc ttc gtc gag ctg atg        576
Ala Met Ile Arg Glu Val Ala Ala Ser Phe Pro Phe Val Glu Leu Met           
            180                 185                 190                   

gat acc tgg acc tgg ttc cag tcc ggc atc aac acc gac ggg gcg cat        624
Asp Thr Trp Thr Trp Phe Gln Ser Gly Ile Asn Thr Asp Gly Ala His           
        195                 200                 205                       

aac ccg gtg acg aac aag aag atc gca tcg ggc gag atc ctg tcg ctg        672
Asn Pro Val Thr Asn Lys Lys Ile Ala Ser Gly Glu Ile Leu Ser Leu           
    210                 215                 220                           

aac tgc ttc ccg atg atc ttc ggc tat tat acc gcg ctg gaa cgc acg        720
Asn Cys Phe Pro Met Ile Phe Gly Tyr Tyr Thr Ala Leu Glu Arg Thr           
225                 230                 235                 240           

atg ttt tgc gac agc gtg gac gat gcc agc ctc gac atc tgg gaa aag        768
Met Phe Cys Asp Ser Val Asp Asp Ala Ser Leu Asp Ile Trp Glu Lys           
                245                 250                 255               

aac gtc gcc gtg cat cgc cgg ggc ctg gaa ctg atc aag ccc ggt gcg        816
Asn Val Ala Val His Arg Arg Gly Leu Glu Leu Ile Lys Pro Gly Ala           
            260                 265                 270                   

aaa tgc aac gag atc gca ttg gag ctc aac gac atg tac cgc cag tgg        864
Lys Cys Asn Glu Ile Ala Leu Glu Leu Asn Asp Met Tyr Arg Gln Trp           
        275                 280                 285                       

gat ctg ctg aaa tat cgc agc ttc ggc tat ggc cac tcc ttc ggc gtc        912
Asp Leu Leu Lys Tyr Arg Ser Phe Gly Tyr Gly His Ser Phe Gly Val           
    290                 295                 300                           

ctg agc cac tat tac ggg cgc gag gcc ggg gtc gag ctg cgc gag gac        960
Leu Ser His Tyr Tyr Gly Arg Glu Ala Gly Val Glu Leu Arg Glu Asp           
305                 310                 315                 320           

atc gag acc gag ctg aag ccc ggc atg gtg gtc tcg atg gaa ccg atg       1008
Ile Glu Thr Glu Leu Lys Pro Gly Met Val Val Ser Met Glu Pro Met           
                325                 330                 335               

gtg atg ttg ccc gag ggt gcg ccc ggc gcg ggc ggc tat cgc gag cat       1056
Val Met Leu Pro Glu Gly Ala Pro Gly Ala Gly Gly Tyr Arg Glu His           
            340                 345                 350                   

gac atc ctg atc gtg acc gag gac ggg gcc gat aac atc acc ggg ttc       1104
Asp Ile Leu Ile Val Thr Glu Asp Gly Ala Asp Asn Ile Thr Gly Phe           
        355                 360                 365                       

ccc ttc ggc ccc gag cac aac atc atc cgc aac tga                       1140
Pro Phe Gly Pro Glu His Asn Ile Ile Arg Asn                               
    370                 375                                               


<210>  46
<211>  379
<212>  PRT
<213>  Paracoccus denitrificans

<400>  46

Met Gln Arg Arg Gln Asp Asp Met Arg Arg Trp Met Ala Gly Asn Gly 
1               5                   10                  15      


Val Asp Ala Ala Leu Phe Thr Ser Tyr His Cys Ile Asn Tyr Tyr Ser 
            20                  25                  30          


Gly Trp Leu Tyr Cys Tyr Phe Gly Arg Lys Tyr Gly Met Val Ile Thr 
        35                  40                  45              


Gln Asp Ala Ala Thr Thr Ile Ser Ala Gly Ile Asp Gly Gly Gln Pro 
    50                  55                  60                  


Trp Arg Arg Ser Phe Gly Gly Asn Val Thr Tyr Thr Asp Trp Arg Arg 
65                  70                  75                  80  


Asp Asn Tyr Phe Arg Ala Val Arg Gln Leu Thr Pro Gly Val Lys Arg 
                85                  90                  95      


Leu Gly Ile Glu Phe Asp His Val Asn Met Asp Leu Arg Arg Gln Leu 
            100                 105                 110         


Glu Ala Ala Leu Pro Gly Val Glu Phe Val Asp Val Gly Gln Pro Ser 
        115                 120                 125             


Met Trp Met Arg Ser Ile Lys Ser Ala Glu Glu His Lys Leu Ile Arg 
    130                 135                 140                 


Glu Gly Ala Arg Ile Cys Asp Val Gly Gly Ala Ala Val Ala Ala Ala 
145                 150                 155                 160 


Val Lys Ala Gly Val Pro Glu His Glu Val Ala Ile Ala Ser Thr Asn 
                165                 170                 175     


Ala Met Ile Arg Glu Val Ala Ala Ser Phe Pro Phe Val Glu Leu Met 
            180                 185                 190         


Asp Thr Trp Thr Trp Phe Gln Ser Gly Ile Asn Thr Asp Gly Ala His 
        195                 200                 205             


Asn Pro Val Thr Asn Lys Lys Ile Ala Ser Gly Glu Ile Leu Ser Leu 
    210                 215                 220                 


Asn Cys Phe Pro Met Ile Phe Gly Tyr Tyr Thr Ala Leu Glu Arg Thr 
225                 230                 235                 240 


Met Phe Cys Asp Ser Val Asp Asp Ala Ser Leu Asp Ile Trp Glu Lys 
                245                 250                 255     


Asn Val Ala Val His Arg Arg Gly Leu Glu Leu Ile Lys Pro Gly Ala 
            260                 265                 270         


Lys Cys Asn Glu Ile Ala Leu Glu Leu Asn Asp Met Tyr Arg Gln Trp 
        275                 280                 285             


Asp Leu Leu Lys Tyr Arg Ser Phe Gly Tyr Gly His Ser Phe Gly Val 
    290                 295                 300                 


Leu Ser His Tyr Tyr Gly Arg Glu Ala Gly Val Glu Leu Arg Glu Asp 
305                 310                 315                 320 


Ile Glu Thr Glu Leu Lys Pro Gly Met Val Val Ser Met Glu Pro Met 
                325                 330                 335     


Val Met Leu Pro Glu Gly Ala Pro Gly Ala Gly Gly Tyr Arg Glu His 
            340                 345                 350         


Asp Ile Leu Ile Val Thr Glu Asp Gly Ala Asp Asn Ile Thr Gly Phe 
        355                 360                 365             


Pro Phe Gly Pro Glu His Asn Ile Ile Arg Asn 
    370                 375                 


<210>  47
<211>  379
<212>  PRT
<213>  Paracoccus denitrificans


<220>
<221>  mat_peptide
<222>  (1)..(379)
<223>  Mutant creatine amidinohydrolase Paracoccus denitrificans _A1B7I6
       with C150A+C274A substitutions

<400>  47

Met Gln Arg Arg Gln Asp Asp Met Arg Arg Trp Met Ala Gly Asn Gly 
1               5                   10                  15      


Val Asp Ala Ala Leu Phe Thr Ser Tyr His Cys Ile Asn Tyr Tyr Ser 
            20                  25                  30          


Gly Trp Leu Tyr Cys Tyr Phe Gly Arg Lys Tyr Gly Met Val Ile Thr 
        35                  40                  45              


Gln Asp Ala Ala Thr Thr Ile Ser Ala Gly Ile Asp Gly Gly Gln Pro 
    50                  55                  60                  


Trp Arg Arg Ser Phe Gly Gly Asn Val Thr Tyr Thr Asp Trp Arg Arg 
65                  70                  75                  80  


Asp Asn Tyr Phe Arg Ala Val Arg Gln Leu Thr Pro Gly Val Lys Arg 
                85                  90                  95      


Leu Gly Ile Glu Phe Asp His Val Asn Met Asp Leu Arg Arg Gln Leu 
            100                 105                 110         


Glu Ala Ala Leu Pro Gly Val Glu Phe Val Asp Val Gly Gln Pro Ser 
        115                 120                 125             


Met Trp Met Arg Ser Ile Lys Ser Ala Glu Glu His Lys Leu Ile Arg 
    130                 135                 140                 


Glu Gly Ala Arg Ile Ala Asp Val Gly Gly Ala Ala Val Ala Ala Ala 
145                 150                 155                 160 


Val Lys Ala Gly Val Pro Glu His Glu Val Ala Ile Ala Ser Thr Asn 
                165                 170                 175     


Ala Met Ile Arg Glu Val Ala Ala Ser Phe Pro Phe Val Glu Leu Met 
            180                 185                 190         


Asp Thr Trp Thr Trp Phe Gln Ser Gly Ile Asn Thr Asp Gly Ala His 
        195                 200                 205             


Asn Pro Val Thr Asn Lys Lys Ile Ala Ser Gly Glu Ile Leu Ser Leu 
    210                 215                 220                 


Asn Cys Phe Pro Met Ile Phe Gly Tyr Tyr Thr Ala Leu Glu Arg Thr 
225                 230                 235                 240 


Met Phe Cys Asp Ser Val Asp Asp Ala Ser Leu Asp Ile Trp Glu Lys 
                245                 250                 255     


Asn Val Ala Val His Arg Arg Gly Leu Glu Leu Ile Lys Pro Gly Ala 
            260                 265                 270         


Lys Ala Asn Glu Ile Ala Leu Glu Leu Asn Asp Met Tyr Arg Gln Trp 
        275                 280                 285             


Asp Leu Leu Lys Tyr Arg Ser Phe Gly Tyr Gly His Ser Phe Gly Val 
    290                 295                 300                 


Leu Ser His Tyr Tyr Gly Arg Glu Ala Gly Val Glu Leu Arg Glu Asp 
305                 310                 315                 320 


Ile Glu Thr Glu Leu Lys Pro Gly Met Val Val Ser Met Glu Pro Met 
                325                 330                 335     


Val Met Leu Pro Glu Gly Ala Pro Gly Ala Gly Gly Tyr Arg Glu His 
            340                 345                 350         


Asp Ile Leu Ile Val Thr Glu Asp Gly Ala Asp Asn Ile Thr Gly Phe 
        355                 360                 365             


Pro Phe Gly Pro Glu His Asn Ile Ile Arg Asn 
    370                 375                 


<210>  48
<211>  379
<212>  PRT
<213>  Paracoccus denitrificans


<220>
<221>  mat_peptide
<222>  (1)..(379)
<223>  Mutant creatine amidinohydrolase Paracoccus denitrificans _A1B7I6
       with C150A+C274A+C243L substitutions

<400>  48

Met Gln Arg Arg Gln Asp Asp Met Arg Arg Trp Met Ala Gly Asn Gly 
1               5                   10                  15      


Val Asp Ala Ala Leu Phe Thr Ser Tyr His Cys Ile Asn Tyr Tyr Ser 
            20                  25                  30          


Gly Trp Leu Tyr Cys Tyr Phe Gly Arg Lys Tyr Gly Met Val Ile Thr 
        35                  40                  45              


Gln Asp Ala Ala Thr Thr Ile Ser Ala Gly Ile Asp Gly Gly Gln Pro 
    50                  55                  60                  


Trp Arg Arg Ser Phe Gly Gly Asn Val Thr Tyr Thr Asp Trp Arg Arg 
65                  70                  75                  80  


Asp Asn Tyr Phe Arg Ala Val Arg Gln Leu Thr Pro Gly Val Lys Arg 
                85                  90                  95      


Leu Gly Ile Glu Phe Asp His Val Asn Met Asp Leu Arg Arg Gln Leu 
            100                 105                 110         


Glu Ala Ala Leu Pro Gly Val Glu Phe Val Asp Val Gly Gln Pro Ser 
        115                 120                 125             


Met Trp Met Arg Ser Ile Lys Ser Ala Glu Glu His Lys Leu Ile Arg 
    130                 135                 140                 


Glu Gly Ala Arg Ile Ala Asp Val Gly Gly Ala Ala Val Ala Ala Ala 
145                 150                 155                 160 


Val Lys Ala Gly Val Pro Glu His Glu Val Ala Ile Ala Ser Thr Asn 
                165                 170                 175     


Ala Met Ile Arg Glu Val Ala Ala Ser Phe Pro Phe Val Glu Leu Met 
            180                 185                 190         


Asp Thr Trp Thr Trp Phe Gln Ser Gly Ile Asn Thr Asp Gly Ala His 
        195                 200                 205             


Asn Pro Val Thr Asn Lys Lys Ile Ala Ser Gly Glu Ile Leu Ser Leu 
    210                 215                 220                 


Asn Cys Phe Pro Met Ile Phe Gly Tyr Tyr Thr Ala Leu Glu Arg Thr 
225                 230                 235                 240 


Met Phe Leu Asp Ser Val Asp Asp Ala Ser Leu Asp Ile Trp Glu Lys 
                245                 250                 255     


Asn Val Ala Val His Arg Arg Gly Leu Glu Leu Ile Lys Pro Gly Ala 
            260                 265                 270         


Lys Ala Asn Glu Ile Ala Leu Glu Leu Asn Asp Met Tyr Arg Gln Trp 
        275                 280                 285             


Asp Leu Leu Lys Tyr Arg Ser Phe Gly Tyr Gly His Ser Phe Gly Val 
    290                 295                 300                 


Leu Ser His Tyr Tyr Gly Arg Glu Ala Gly Val Glu Leu Arg Glu Asp 
305                 310                 315                 320 


Ile Glu Thr Glu Leu Lys Pro Gly Met Val Val Ser Met Glu Pro Met 
                325                 330                 335     


Val Met Leu Pro Glu Gly Ala Pro Gly Ala Gly Gly Tyr Arg Glu His 
            340                 345                 350         


Asp Ile Leu Ile Val Thr Glu Asp Gly Ala Asp Asn Ile Thr Gly Phe 
        355                 360                 365             


Pro Phe Gly Pro Glu His Asn Ile Ile Arg Asn 
    370                 375                 


