                               SEQUENCE LISTING

<110> RESEARCH INSTITUTE AT NATIONWIDE CHILDREN'S HOSPITAL
      OHIO STATE INNOVATION FOUNDATION
 
<120> LIVE ATTENUATED VACCINES FOR PNEUMOVIRUSES AND RELATED METHODS 
      AND MATERIALS

<130> RINCH02WOU1

<140>
<141>

<150> 62/118,752
<151> 2015-02-20

<160> 9     

<170> PatentIn version 3.5

<210> 1
<211> 187
<212> PRT
<213> Human metapneumovirus

<400> 1
Met Ser Arg Lys Ala Pro Cys Lys Tyr Glu Val Arg Gly Lys Cys Asn 
1               5                   10                  15      


Arg Gly Ser Glu Cys Lys Phe Asn His Asn Tyr Trp Ser Trp Pro Asp 
            20                  25                  30          


Arg Tyr Leu Leu Ile Arg Ser Asn Tyr Leu Leu Asn Gln Leu Leu Arg 
        35                  40                  45              


Asn Thr Asp Arg Ala Asp Gly Leu Ser Ile Ile Ser Gly Ala Gly Arg 
    50                  55                  60                  


Glu Asp Arg Thr Gln Asp Phe Val Leu Gly Ser Thr Asn Val Val Gln 
65                  70                  75                  80  


Gly Tyr Ile Asp Asp Asn Gln Ser Ile Thr Lys Ala Ala Ala Cys Tyr 
                85                  90                  95      


Ser Leu His Asn Ile Ile Lys Gln Leu Gln Glu Val Glu Val Arg Gln 
            100                 105                 110         


Ala Arg Asp Ser Lys Leu Ser Asp Ser Lys His Val Ala Leu His Asn 
        115                 120                 125             


Leu Ile Leu Ser Tyr Met Glu Met Ser Lys Thr Pro Ala Ser Leu Ile 
    130                 135                 140                 


Asn Asn Leu Lys Arg Leu Pro Arg Glu Lys Leu Lys Lys Leu Ala Lys 
145                 150                 155                 160 


Leu Ile Ile Asp Leu Ser Ala Gly Ala Asp Asn Asp Ser Ser Tyr Ala 
                165                 170                 175     


Leu Gln Asp Ser Glu Ser Thr Asn Gln Val Gln 
            180                 185         


<210> 2
<211> 187
<212> PRT
<213> Human metapneumovirus

<400> 2
Met Ser Arg Lys Ala Pro Cys Lys Tyr Glu Val Arg Gly Lys Cys Asn 
1               5                   10                  15      


Arg Gly Ser Glu Cys Lys Phe Asn His Asn Tyr Trp Ser Trp Pro Asp 
            20                  25                  30          


Arg Tyr Leu Leu Leu Arg Ser Asn Tyr Leu Leu Asn Gln Leu Leu Arg 
        35                  40                  45              


Asn Thr Asp Lys Ala Asp Gly Leu Ser Ile Ile Ser Gly Ala Gly Arg 
    50                  55                  60                  


Glu Asp Arg Thr Gln Asp Phe Val Leu Gly Ser Thr Asn Val Val Gln 
65                  70                  75                  80  


Gly Tyr Ile Asp Asn Asn Gln Gly Ile Thr Lys Ala Ala Ala Cys Tyr 
                85                  90                  95      


Ser Leu His Asn Ile Ile Lys Gln Leu Gln Glu Ile Glu Val Arg Gln 
            100                 105                 110         


Ala Arg Asp Asn Lys Leu Ser Asp Ser Lys His Val Ala Leu His Asn 
        115                 120                 125             


Leu Ile Leu Ser Tyr Met Glu Met Ser Lys Thr Pro Ala Ser Leu Ile 
    130                 135                 140                 


Asn Asn Leu Lys Lys Leu Pro Arg Glu Lys Leu Lys Lys Leu Ala Lys 
145                 150                 155                 160 


Leu Ile Ile Asp Leu Ser Ala Gly Thr Asp Asn Asp Ser Ser Tyr Ala 
                165                 170                 175     


Leu Gln Asp Ser Glu Ser Thr Asn Gln Val Gln 
            180                 185         


<210> 3
<211> 186
<212> PRT
<213> Avian metapneumovirus

<400> 3
Met Ser Arg Arg Asn Pro Cys Arg Tyr Glu Ile Arg Gly Lys Cys Asn 
1               5                   10                  15      


Arg Gly Ser Ser Cys Thr Phe Asn His Asn Tyr Trp Ser Trp Pro Asp 
            20                  25                  30          


His Val Leu Leu Val Arg Ala Asn Tyr Met Leu Asn Gln Leu Leu Arg 
        35                  40                  45              


Asn Thr Asp Arg Thr Asp Gly Leu Ser Leu Ile Ser Gly Ala Gly Arg 
    50                  55                  60                  


Glu Asp Arg Thr Gln Asp Phe Val Leu Gly Ser Ala Asn Val Val Gln 
65                  70                  75                  80  


Asn Tyr Ile Glu Gly Asn Thr Thr Ile Thr Lys Ser Ala Ala Cys Tyr 
                85                  90                  95      


Ser Leu Tyr Asn Ile Ile Lys Gln Leu Gln Glu Asn Asp Val Lys Thr 
            100                 105                 110         


Ser Arg Asp Ser Met Leu Glu Asp Pro Lys His Val Ala Leu His Asn 
        115                 120                 125             


Leu Ile Leu Ser Tyr Val Asp Met Ser Lys Asn Pro Ala Ser Leu Ile 
    130                 135                 140                 


Asn Ser Leu Lys Arg Leu Pro Arg Glu Lys Leu Lys Lys Leu Ala Lys 
145                 150                 155                 160 


Ile Ile Leu Gln Leu Ser Ala Gly Pro Glu Ser Asp Asn Ala Ser Gly 
                165                 170                 175     


Asn Thr Leu Gln Lys Gly Asp Ser Asn Asn 
            180                 185     


<210> 4
<211> 186
<212> PRT
<213> Avian metapneumovirus

<400> 4
Met Ser Arg Arg Asn Pro Cys Arg Tyr Glu Thr Arg Gly Lys Cys Asn 
1               5                   10                  15      


Arg Gly Ser Ser Cys Thr Phe Asn His Asn Tyr Trp Ser Trp Pro Asp 
            20                  25                  30          


His Val Leu Leu Val Arg Ala Asn Tyr Met Leu Asn Gln Leu Val Arg 
        35                  40                  45              


Asn Thr Asp Arg Thr Asp Gly Leu Ser Leu Ile Ser Gly Ala Gly Arg 
    50                  55                  60                  


Glu Asp Arg Thr Gln Asp Phe Val Leu Gly Ser Ala Asn Val Val Gln 
65                  70                  75                  80  


Asn Tyr Ile Glu Gly Asn Ala Thr Ile Thr Lys Ser Ala Ala Cys Tyr 
                85                  90                  95      


Ser Leu Tyr Asn Ile Ile Lys Gln Leu Gln Glu Asn Asp Val Lys Ser 
            100                 105                 110         


Ala Arg Asp Leu Met Val Asp Asp Pro Lys His Val Ala Leu His Asn 
        115                 120                 125             


Leu Val Leu Ser Tyr Ile Asp Met Ser Lys Asn Pro Ala Asn Leu Ile 
    130                 135                 140                 


Asn Ser Leu Lys Arg Leu Pro Lys Glu Lys Leu Lys Lys Leu Ala Lys 
145                 150                 155                 160 


Ile Ile Ile Gln Leu Ser Ala Gly Ser Glu Gly Glu Asn Ala Asn Ser 
                165                 170                 175     


Asn Thr Leu Gln Lys Gly Asp Ser Ser Asn 
            180                 185     


<210> 5
<211> 184
<212> PRT
<213> Avian metapneumovirus

<400> 5
Met Ser Arg Lys Ala Pro Cys Lys Tyr Glu Val Arg Gly Lys Cys Asn 
1               5                   10                  15      


Arg Gly Ser Glu Cys Lys Phe Asn His Asn Tyr Trp Asn Trp Pro Asp 
            20                  25                  30          


Arg Tyr Leu Leu Leu Arg Ser Asn Tyr Leu Leu Asn Gln Leu Leu Arg 
        35                  40                  45              


Asn Thr Asp Arg Ser Asp Gly Leu Ser Leu Ile Ser Gly Ala Gly Arg 
    50                  55                  60                  


Asp Asp Arg Thr Gln Asp Phe Val Leu Gly Ser Thr Asn Val Val Gln 
65                  70                  75                  80  


Asn Tyr Ile Asp Asn Asn Glu Asn Ile Thr Lys Ala Ser Ala Cys Tyr 
                85                  90                  95      


Ser Leu Tyr Asn Ile Ile Lys Gln Leu Gln Glu Thr Asp Val Arg Gln 
            100                 105                 110         


Ala Arg Asp Asn Lys Val Asp Asp Ser Lys His Val Ala Leu His Asn 
        115                 120                 125             


Leu Val Leu Ser Tyr Met Glu Met Ser Lys Thr Pro Ala Ser Leu Ile 
    130                 135                 140                 


Asn Asn Leu Lys Lys Leu Pro Lys Glu Lys Leu Lys Lys Leu Ala Lys 
145                 150                 155                 160 


Leu Ile Ile Glu Leu Ser Ala Gly Val Glu Asn Asp Ser Thr Ala Ala 
                165                 170                 175     


Met Gln Asp Ser Ala Asn Ser Asp 
            180                 


<210> 6
<211> 194
<212> PRT
<213> Human respiratory syncytial virus

<400> 6
Met Ser Arg Arg Asn Pro Cys Lys Phe Glu Ile Arg Gly His Cys Leu 
1               5                   10                  15      


Asn Gly Lys Arg Cys His Phe Ser His Asn Tyr Phe Glu Trp Pro Pro 
            20                  25                  30          


His Ala Leu Leu Val Arg Gln Asn Phe Met Leu Asn Arg Ile Leu Lys 
        35                  40                  45              


Ser Met Asp Lys Ser Ile Asp Thr Leu Ser Glu Ile Ser Gly Ala Ala 
    50                  55                  60                  


Glu Leu Asp Arg Thr Glu Glu Tyr Ala Leu Gly Val Val Gly Val Leu 
65                  70                  75                  80  


Glu Ser Tyr Ile Gly Ser Ile Asn Asn Ile Thr Lys Gln Ser Ala Cys 
                85                  90                  95      


Val Ala Met Ser Lys Leu Leu Thr Glu Leu Asn Ser Asp Asp Ile Lys 
            100                 105                 110         


Lys Leu Arg Asp Asn Glu Glu Leu Asn Ser Pro Lys Ile Arg Val Tyr 
        115                 120                 125             


Asn Thr Val Ile Ser Tyr Ile Glu Ser Asn Arg Lys Asn Asn Lys Gln 
    130                 135                 140                 


Thr Ile His Leu Leu Lys Arg Leu Pro Ala Asp Val Leu Lys Lys Thr 
145                 150                 155                 160 


Ile Lys Asn Thr Leu Asp Ile His Lys Ser Ile Thr Ile Asn Asn Pro 
                165                 170                 175     


Lys Glu Ser Thr Val Ser Asp Thr Asn Asp His Ala Lys Asn Asn Asp 
            180                 185                 190         


Thr Thr 
        


<210> 7
<211> 195
<212> PRT
<213> Human respiratory syncytial virus

<400> 7
Met Ser Arg Arg Asn Pro Cys Lys Phe Glu Ile Arg Gly His Cys Leu 
1               5                   10                  15      


Asn Gly Arg Arg Cys His Tyr Ser His Asn Tyr Phe Glu Trp Pro Pro 
            20                  25                  30          


His Ala Leu Leu Val Arg Gln Asn Phe Met Leu Asn Lys Ile Leu Lys 
        35                  40                  45              


Ser Met Asp Lys Ser Ile Asp Thr Leu Ser Glu Ile Ser Gly Ala Ala 
    50                  55                  60                  


Glu Leu Asp Arg Thr Glu Glu Tyr Ala Leu Gly Ile Val Gly Val Leu 
65                  70                  75                  80  


Glu Ser Tyr Ile Gly Ser Ile Asn Asn Ile Thr Lys Gln Ser Ala Cys 
                85                  90                  95      


Val Ala Met Ser Lys Leu Leu Ile Glu Ile Asn Ser Asp Asp Ile Lys 
            100                 105                 110         


Lys Leu Arg Asp Asn Glu Glu Pro Asn Ser Pro Lys Ile Arg Val Tyr 
        115                 120                 125             


Asn Thr Val Ile Ser Tyr Ile Glu Ser Asn Arg Lys Asn Asn Lys Gln 
    130                 135                 140                 


Thr Ile His Leu Leu Lys Arg Leu Pro Ala Asp Val Leu Lys Lys Thr 
145                 150                 155                 160 


Ile Lys Asn Thr Leu Asp Ile His Lys Ser Ile Ile Ile Ser Asn Pro 
                165                 170                 175     


Lys Glu Ser Thr Val Asn Asp Gln Asn Asp Gln Thr Lys Asn Asn Asp 
            180                 185                 190         


Ile Thr Gly 
        195 


<210> 8
<211> 186
<212> PRT
<213> Bovine respiratory syncytial virus

<400> 8
Met Ser Arg Arg Asn Pro Cys Lys Tyr Glu Ile Arg Gly His Cys Leu 
1               5                   10                  15      


Asn Gly Lys Lys Cys His Phe Ser His Asn Tyr Phe Glu Trp Pro Pro 
            20                  25                  30          


His Ala Leu Leu Val Arg Gln Asn Phe Met Leu Asn Lys Ile Leu Lys 
        35                  40                  45              


Ser Met Asp Arg Asn Asn Asp Thr Leu Ser Glu Ile Ser Gly Ala Ala 
    50                  55                  60                  


Glu Leu Asp Arg Thr Glu Glu Tyr Ala Leu Gly Val Ile Gly Val Leu 
65                  70                  75                  80  


Glu Ser Tyr Leu Ser Ser Ile Asn Asn Ile Thr Lys Gln Ser Ala Cys 
                85                  90                  95      


Val Ala Met Ser Lys Leu Leu Ala Glu Ile Asn Asn Asp Asp Ile Lys 
            100                 105                 110         


Arg Leu Arg Asn Lys Glu Val Pro Thr Ser Pro Lys Ile Arg Ile Tyr 
        115                 120                 125             


Asn Thr Val Ile Ser Tyr Ile Asp Ser Asn Lys Arg Asn Thr Lys Gln 
    130                 135                 140                 


Thr Ile His Leu Leu Lys Arg Leu Pro Ala Asp Val Leu Lys Lys Thr 
145                 150                 155                 160 


Ile Lys Asn Thr Ile Asp Ile His Asn Glu Ile Asn Gly Asn Asn Gln 
                165                 170                 175     


Gly Asp Ile Asn Val Asp Glu Gln Asn Glu 
            180                 185     


<210> 9
<211> 176
<212> PRT
<213> Pneumonia virus 


<220>
<223> Pneumonia virus of mice

<400> 9
Met Ser Val Arg Pro Cys Lys Phe Glu Val Gln Gly Phe Cys Ser Arg 
1               5                   10                  15      


Gly Arg Asn Cys Lys Tyr Ser His Lys Tyr Trp Glu Trp Pro Leu Lys 
            20                  25                  30          


Thr Leu Met Leu Arg Gln Asn Tyr Met Leu Asn Arg Ile Tyr Arg Phe 
        35                  40                  45              


Leu Asp Thr Asn Thr Asp Ala Met Ser Asp Val Ser Gly Phe Asp Ala 
    50                  55                  60                  


Pro Gln Arg Thr Ala Glu Tyr Ala Leu Gly Thr Ile Gly Val Leu Lys 
65                  70                  75                  80  


Ser Tyr Leu Glu Lys Thr Asn Asn Ile Thr Lys Ser Ile Ala Cys Gly 
                85                  90                  95      


Ser Leu Ile Thr Val Leu Gln Asn Leu Asp Val Gly Leu Val Ile Gln 
            100                 105                 110         


Ala Arg Asp Ser Asn Thr Glu Asp Thr Asn Tyr Leu Arg Ser Cys Asn 
        115                 120                 125             


Thr Ile Leu Ser Tyr Ile Asp Lys Ile His Lys Lys Arg Gln Ile Ile 
    130                 135                 140                 


His Ile Leu Lys Arg Leu Pro Val Gly Val Leu Cys Asn Leu Ile Gln 
145                 150                 155                 160 


Ser Val Ile Ser Ile Glu Glu Lys Ile Asn Ser Ser Met Lys Thr Glu 
                165                 170                 175     

