                         SEQUENCE LISTING

<110>  Board of Regents, The University of Texas System
       Zhong, Guangming
 
<120>  METHODS AND COMPOSITIONS FOR ATTENUATED CHLAMYDIA AS VACCINE AND 
       VECTOR

<130>  9237-54WO

<150>  US 62/118,961
<151>  2015-02-20

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  159
<212>  PRT
<213>  Chlamydia trachomatis

<400>  1

Met Ser Ala Ala Thr Ser Gln Ile Gly Asp Thr Gln Tyr Val Ser Ser 
1               5                   10                  15      


Leu Pro Pro Leu Glu Pro Leu Gly Thr Pro Pro Ile Ala Glu Leu Leu 
            20                  25                  30          


Phe Ser Ile Tyr Ser Leu Leu Leu Glu Ala Val Glu Ile Arg Gln Glu 
        35                  40                  45              


Thr Ile Leu Thr Gln Ser Lys Gln Leu Asn Asp Asn Thr Asn Ile Gln 
    50                  55                  60                  


Gln Gln Leu Asn Gln Glu Thr Asn Gln Ile Lys Tyr Ala Val Val Gly 
65                  70                  75                  80  


Ser Gly Ala Lys Glu Asp Glu Ile Thr Arg Val Gln Asn Gln Asn Gln 
                85                  90                  95      


Asn Tyr Ser Ala Gln Arg Ser Asn Ile Gln Asp Gln Leu Val Thr Ala 
            100                 105                 110         


Arg Gln Asn Gly Gln Ile Ile Leu Ser His Ala Ser Thr Asn Ile Asn 
        115                 120                 125             


Ile Met Gln Gln Ile Ala Gln Gln Asn Ser Ser Phe Ile Lys Thr Leu 
    130                 135                 140                 


Asn Ser Val Gly Ser Thr Val Asn Gln Leu Asn Lys Pro Leu Ser 
145                 150                 155                 


<210>  2
<211>  408
<212>  PRT
<213>  Chlamydia trachomatis

<400>  2

Met Met Lys Pro Leu Arg Phe Gly Tyr Phe Phe Cys Ala Ile Tyr Phe 
1               5                   10                  15      


Thr Leu Leu Gln Ala Ala Phe Ala Lys Glu Pro Asn Ser Cys Pro Asp 
            20                  25                  30          


Cys Gln Asn Asn Trp Lys Glu Val Thr His Thr Asp Gln Leu Pro Glu 
        35                  40                  45              


Asn Ile Ile His Ala Asp Asp Ala Cys Tyr His Ser Gly Tyr Val Gln 
    50                  55                  60                  


Ala Leu Ile Asp Met His Phe Leu Asp Ser Cys Cys Gln Val Ile Val 
65                  70                  75                  80  


Glu Asn Gln Thr Ala Tyr Leu Phe Ser Leu Pro Thr Asp Asp Val Thr 
                85                  90                  95      


Arg Asn Ala Ile Ile Asn Leu Ile Lys Asp Leu Pro Phe Ile His Ser 
            100                 105                 110         


Val Glu Ile Cys Gln Ala Ser Tyr Gln Thr Cys His His Gln Gly Pro 
        115                 120                 125             


His Gly Lys Thr Ser Leu Pro Glu Gln Arg Ser Phe Cys Thr Lys Val 
    130                 135                 140                 


Cys Gly Lys Glu Ala Ile Trp Leu Pro Gln Asn Thr Ile Leu Phe Ser 
145                 150                 155                 160 


Pro Leu Val Ala Asp Pro Arg Gln Ala Thr Asn Ser Ala Gly Ile Arg 
                165                 170                 175     


Phe Asn Asp Glu Val Leu Gly Lys Arg Val Gly Ser Ala Thr Phe Gly 
            180                 185                 190         


Gly Asp Phe Ile Phe Leu Arg Leu Phe Asp Ile Ser Arg Phe His Gly 
        195                 200                 205             


Asp Met Asp Ile Gly Leu Gln Gly Ala Val Phe Ser Val Phe Asp Leu 
    210                 215                 220                 


Asp His Pro Glu Ala Cys Met Val Asn Ser Asp Phe Phe Val Ala Ala 
225                 230                 235                 240 


Leu Cys Asn Phe Ala Val Asn Lys Trp Ser Tyr Arg Phe Arg Leu Trp 
                245                 250                 255     


His Leu Ser Ser His Leu Gly Asp Glu Phe Ile Leu Ala Asn Gln Leu 
            260                 265                 270         


Pro Pro Lys Lys Arg Tyr Asn Arg Ser Asp Glu Ala Val Asp Phe Phe 
        275                 280                 285             


Ala Ser Phe Arg Tyr Thr Pro Gln Ile Arg Val Tyr Gly Gly Ile Gly 
    290                 295                 300                 


Tyr Ile Ile Ser Arg Asp Leu Thr Phe Pro Glu Asp Pro Leu Tyr Phe 
305                 310                 315                 320 


Glu Gly Gly Ile Glu Leu Arg Pro Phe Gly Leu Arg Glu Asp Asn Leu 
                325                 330                 335     


His Ala Gln Pro Val Phe Ala Met His Phe Arg Phe Trp Glu Glu His 
            340                 345                 350         


Asp Phe Ser Ile Asp Gln Thr Tyr Ile Val Gly Met Glu Trp Ser Lys 
        355                 360                 365             


Phe Gln Asp Val Gly Arg Lys Val Arg Ala Val Leu Glu Tyr His Gln 
    370                 375                 380                 


Gly Phe Ser His Glu Gly Gln Phe Val Arg Glu Glu Cys Asp Tyr Tyr 
385                 390                 395                 400 


Gly Phe Arg Leu Ser Tyr Gly Phe 
                405             


<210>  3
<211>  360
<212>  PRT
<213>  Chlamydia trachomatis

<400>  3

Met Val Ser Phe Asp Leu Asn Asp Pro Val Arg Asn Thr Asp Asn His 
1               5                   10                  15      


Tyr Arg Asn Ile Asn Arg Met Leu Asn Ser Ala Thr Cys Ala Ala Gly 
            20                  25                  30          


Gly Ile Gly Leu Leu Thr Pro Val Val Cys Ser Pro Met Gly Ala Phe 
        35                  40                  45              


Cys Phe Ala Gln Gly Pro Ser Ser Ala Glu Asp Leu Gly His Arg Ile 
    50                  55                  60                  


Gln His Phe Val Ala Cys Ser Gly Pro Ala Ala Gly Phe Tyr Ser Leu 
65                  70                  75                  80  


Ser Asn Glu Arg Ile Met Phe Glu Glu Ala Ala Val Pro Ser Val Leu 
                85                  90                  95      


Glu Ala Val Glu Ala Thr Phe Trp Ile Ser Ala Phe Ala Arg Leu Arg 
            100                 105                 110         


Gly Asn Glu Pro Ser Thr Cys Asp Thr Val Met Met Ser Cys Val Ile 
        115                 120                 125             


Gly Cys Ile Ser Leu Val Cys Gly Ala Met Phe Val Ala Ile Val Ser 
    130                 135                 140                 


Cys Ala Val Lys Ile Ser Arg Ile Val Arg Thr Met Thr Gln Ala His 
145                 150                 155                 160 


Ala Leu Arg Glu Thr Ile Gln Arg Gln Leu Ala Ala Arg Ala Thr Asp 
                165                 170                 175     


Met Arg Ser Ala Tyr Ser Lys Leu Lys Gly Ile Ile Ala Ile Arg Ala 
            180                 185                 190         


Leu Asn Glu Val Glu Arg Gly His Arg Lys Leu Arg Asn Lys Met Ile 
        195                 200                 205             


Thr Ala Phe Val Ala Asn Ala Leu Ile Thr Leu Ala Phe Cys Ala Leu 
    210                 215                 220                 


Leu Ala Ser Ala Val Ile Ala Ala Phe Phe Phe Gly Ala Ala Ser Ala 
225                 230                 235                 240 


Gly Leu Ala Ser Val Phe Phe Gly Cys Leu Trp Gly Gly Ile Gly Ala 
                245                 250                 255     


Leu Ala Val Gly Val Leu Val Gly Ile Val Ser Gly Ile Cys Gln Arg 
            260                 265                 270         


Asn Tyr Lys Val Glu Ala Ala Arg Cys Ile Gln Arg Gly Ala Leu Tyr 
        275                 280                 285             


Ala Leu Val Leu Glu Lys Met Gln Arg Phe Pro Lys Glu Phe Leu Lys 
    290                 295                 300                 


Asp Gly Val Ala Lys Ser Val Val Ala Ile Gln Ala Gly Glu Ser Leu 
305                 310                 315                 320 


Asp Thr Gly Glu Leu Ala Trp Glu Glu Met Pro Ser Ile Thr Ala Cys 
                325                 330                 335     


Leu Gly Arg Glu Gly Met Asp Ala Gln Ala Tyr Ser Phe Leu Ser Ala 
            340                 345                 350         


Ser Pro Leu Asp Ala Arg Ile Glu 
        355                 360 


<210>  4
<211>  159
<212>  PRT
<213>  Chlamydia muridarum

<400>  4

Met Ser Ala Pro Ile Pro Pro Ala Lys Asp Ser Lys Tyr Val Ser Ala 
1               5                   10                  15      


Leu Pro Pro Leu Glu Pro Leu Arg Thr Pro Pro Met Ala Glu Leu Leu 
            20                  25                  30          


Phe Gly Ile Tyr Ser Leu Leu Leu Glu Ala Val Glu Ile Arg Gln Gln 
        35                  40                  45              


Thr Val Leu Thr Gln Ser Gln Gln Leu Asn Asp Asn Thr Asn Ile Gln 
    50                  55                  60                  


Gln Gln Leu Asn Gln Glu Thr Asn Arg Ile Lys Tyr Ala Val Val Asn 
65                  70                  75                  80  


Ala Gly Ala Lys Glu Asp Glu Ile Thr Arg Val Gln Asn Gln Asn Gln 
                85                  90                  95      


Asn Tyr Ser Ala Gln Arg Ser Asn Ile Gln Asp Gln Leu Val Thr Ala 
            100                 105                 110         


Arg Gln Asn Gly Gln Ile Ile Leu Ser His Ala Ser Thr Asn Ile Asn 
        115                 120                 125             


Ile Met Gln Gln Ile Ala Gln Met Asn Ser Ser Phe Ile Lys Thr Leu 
    130                 135                 140                 


Asn Ser Val Gly Ser Thr Val Asn Gln Leu Asn Lys Pro Leu Ser 
145                 150                 155                 


<210>  5
<211>  408
<212>  PRT
<213>  Chlamydia muridarum

<400>  5

Met Met Glu Pro Leu Arg Phe Gly Tyr Phe Phe Cys Ile Leu Phe Ile 
1               5                   10                  15      


Gly Leu Leu Gln Thr Val Phe Ala Lys Glu Pro Asp Ser Cys Pro Asp 
            20                  25                  30          


Cys Lys Asn Asn Trp Lys Glu Val Thr His Thr Asp Gln Leu Pro Glu 
        35                  40                  45              


Asn Ile Ile His Ala Asp Asp Ala Cys Tyr His Ser Gly Tyr Val Gln 
    50                  55                  60                  


Ala Leu Ile Asp Met His Phe Leu Asp Ser Cys Cys Gln Val Ile Val 
65                  70                  75                  80  


Glu Asp Gln Thr Ala Tyr Leu Phe Ser Leu Pro Gln Asp Asp Val Thr 
                85                  90                  95      


Arg Asn Ala Ile Ile Asn Leu Ile Lys Asp Leu Pro Phe Ile Gln Ser 
            100                 105                 110         


Val Glu Ile Cys Gln Ala Ser Tyr Gln Thr Cys His His Gln Gly Pro 
        115                 120                 125             


Tyr Gly Lys Ser Ser Leu Pro Glu Gln Arg Ser Phe Cys Thr Lys Val 
    130                 135                 140                 


Cys Gly Lys Glu Ala Ile Trp Leu Pro Gln Asn Thr Ile Leu Phe Thr 
145                 150                 155                 160 


Pro Leu Val Ala Asp Pro Arg Gln Ala Thr Asn Ser Ala Gly Ile Arg 
                165                 170                 175     


Phe Asn Asp Glu Val Ile Gly Lys Arg Val Gly Ser Ala Val Phe Gly 
            180                 185                 190         


Gly Asp Phe Ile Phe Leu Arg Leu Phe Asp Val Ser Arg Phe His Gly 
        195                 200                 205             


Asp Met Asp Ile Gly Leu Gln Gly Ala Val Phe Ser Val Phe Asp Leu 
    210                 215                 220                 


Asp Asn Pro Asp Ala Cys Met Val Asn Ser Asp Phe Phe Val Ser Ala 
225                 230                 235                 240 


Leu Leu Ser Phe Ala Val Asn Lys Trp Ser Tyr Arg Leu Arg Leu Trp 
                245                 250                 255     


His Leu Ser Ser His Leu Gly Asp Glu Phe Ile Leu Ala Asn Gln Leu 
            260                 265                 270         


Pro Pro Gly Lys Arg Tyr Asn Arg Ser Asp Glu Ala Val Asp Phe Phe 
        275                 280                 285             


Ala Ser Phe Arg Tyr Thr Pro Gln Ile Arg Val Tyr Gly Gly Ile Gly 
    290                 295                 300                 


Tyr Ile Ile Ser Arg Asp Leu Thr Phe Pro Glu Asp Pro Leu Tyr Phe 
305                 310                 315                 320 


Glu Gly Gly Leu Glu Leu Arg Pro Phe Gly Leu Arg Glu Asp Asn Leu 
                325                 330                 335     


His Ala Gln Pro Ile Phe Ala Met His Phe Arg Phe Trp Gly Glu His 
            340                 345                 350         


Asp Phe Ser Ile Asp Gln Thr Tyr Ile Leu Gly Met Glu Trp Ser Lys 
        355                 360                 365             


Phe Gln Asp Val Gly Arg Lys Ile Arg Ala Val Leu Glu Tyr His Gln 
    370                 375                 380                 


Gly Phe Ser His Glu Gly Gln Phe Val Arg Glu Glu Cys Asp Tyr Tyr 
385                 390                 395                 400 


Gly Phe Arg Leu Ser Tyr Gly Phe 
                405             


<210>  6
<211>  365
<212>  PRT
<213>  Chlamydia muridarum

<400>  6

Met Val Ser Phe Asp Leu Ser Val Thr Thr Thr Asn Ile Gly Ala Gly 
1               5                   10                  15      


Tyr Asp Asp Ile Gln Arg Met Leu Asn Gly Val Thr Cys Ser Ser Gly 
            20                  25                  30          


Gly Met Gly Leu Leu Thr Pro Ser Ala Cys Ser Pro Met Ser Ser Phe 
        35                  40                  45              


Cys Ser Ser Asn Gln Pro Tyr Ser Ala Arg Asp Leu Lys Asn Arg Ile 
    50                  55                  60                  


His Gln Phe Cys Gln His Ser Gly Pro Ile Thr Gly Phe Tyr Ser Leu 
65                  70                  75                  80  


Tyr Asn Glu Lys Ile Met Phe Glu Glu Ala Leu Leu Val Pro Thr Val 
                85                  90                  95      


Leu Glu Ala Val Glu Ser Thr Phe Trp Ile Ser Ala Leu Ser Arg Leu 
            100                 105                 110         


Gly Gly Glu Arg Pro Ser Thr Phe Asp Thr Val Ile Leu Ser Phe Phe 
        115                 120                 125             


Val Gly Leu Ile Ser Leu Val Cys Gly Ala Met Phe Val Gly Ile Val 
    130                 135                 140                 


Ser Ser Ala Val Lys Ile Tyr Arg Leu Met Gln Thr Met Arg Gln Ala 
145                 150                 155                 160 


Arg Thr Leu Asn Glu Asn Val Gln Arg Leu Leu Ala Pro Gln Ala Thr 
                165                 170                 175     


Asn Met Arg Ser Ala Phe Ala Lys Leu Lys Gly Ile Val Ala Ser Lys 
            180                 185                 190         


Ala Leu Asp Gln Val Glu Gln Gly Tyr Arg Lys Phe Arg Asn Arg Met 
        195                 200                 205             


Ile Thr Ser Phe Val Ala Asn Ala Leu Ile Thr Ile Ala Phe Cys Ala 
    210                 215                 220                 


Leu Leu Ala Ser Val Ile Leu Ser Ala Phe Phe Ile Gly Gly Ala Ser 
225                 230                 235                 240 


Gly Cys Leu Met Ala Ala Phe Phe Gly Cys Leu Gly Val Gly Leu Gly 
                245                 250                 255     


Ser Leu Thr Ile Gly Met Leu Val Gly Ile Val Ser Ala Ile Cys Gln 
            260                 265                 270         


Arg Lys His Lys Gln Glu Ala Ala Arg Cys Ile Gln Arg Gly Ile Phe 
        275                 280                 285             


Tyr Ser Leu Ile Leu Glu Gln Met Gln Arg Phe Pro Lys Asp Phe Phe 
    290                 295                 300                 


Arg Asp Pro Val Ala Lys Ser Ile Met Ala Ile Gln Ala Gly Glu Ala 
305                 310                 315                 320 


Leu Asp Glu Gly Lys Leu Ser Trp Lys Glu Met Pro Ser Ile Thr Ala 
                325                 330                 335     


Cys Leu Gly Arg Glu Gly Leu Asp Ala Gln Ala Tyr Ser Phe Ile Ser 
            340                 345                 350         


Ser Ser Pro Leu Asp Gly Arg Ile Glu Glu Ala Phe Arg 
        355                 360                 365 


