                         SEQUENCE LISTING

<110>  Bio-Rad Laboratories, Inc.
       Maar, Dianna
       Cooper, Samantha
       Wei, Yang
 
<120>  SMALL NUCLEIC ACID QUANTIFICATION USING SPLIT CYCLE AMPLIFICATION

<130>  1002066

<150>  US 62/117,381
<151>  2015-02-17

<160>  35    

<170>  PatentIn version 3.5

<210>  1
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide primer


<220>
<221>  misc_feature
<222>  (1)..(10)
<223>  n is a, c, g or t; n may be present/absent

<220>
<221>  misc_feature
<222>  (12)..(30)
<223>  n is t; may be present or absent

<220>
<221>  misc_feature
<222>  (33)..(51)
<223>  n is t; may be present or absent

<220>
<221>  misc_feature
<222>  (52)..(61)
<223>  n is a, c, g or t; n may be present/absent (e.g., 1-5, 2-5, or 
       2-10)

<220>
<221>  misc_feature
<222>  (52)..(61)
<223>  sequence is complementary to a portion of a non-polyA region 
       adjacent a the polyA tail

<400>  1
nnnnnnnnnn tnnnnnnnnn nnnnnnnnnn ytnnnnnnnn nnnnnnnnnn nnnnnnnnnn       60

n                                                                       61


<210>  2
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic RT primer sequence

<400>  2
tttttctttt tacgc                                                        15


<210>  3
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR primer complement to RT primer

<400>  3
gcgtaaaaag aaaaa                                                        15


<210>  4
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic hsa-let-7a-5p alignment sequence

<400>  4
aactatacaa cctactacct ca                                                22


<210>  5
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic hsa-let-7b-5p alignment sequence

<400>  5
aaccacacaa cctactacct ca                                                22


<210>  6
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic hsa-let-7c-5p alignment sequence

<400>  6
aaccatacaa cctactacct ca                                                22


<210>  7
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic hsa-let-7d-5p alignment sequence

<400>  7
aactatgcaa cctactacct ct                                                22


<210>  8
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic hsa-let-7e-5p alignment sequence

<400>  8
aactatacaa cctcctacct ca                                                22


<210>  9
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic hsa-let-7f-5p alignment sequence

<400>  9
aactatacaa tctactacct ca                                                22


<210>  10
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic hsa-let-7g-5p alignment sequence

<400>  10
aactgtacaa actactacct ca                                                22


<210>  11
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic hsa-let-7i-5p alignment sequence

<400>  11
aacagcacaa actactacct ca                                                22


<210>  12
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic hsa-miR-98-5p alignment sequence

<400>  12
aacaatacaa cttactacct ca                                                22


<210>  13
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic target DNA sequence 5' to 3' - complement

<400>  13
tcccgctggc aactaacggt gacagacgtg ggct                                   34


<210>  14
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic target sequence 5' to 3'

<400>  14
agcccacgtc tgtcaccgtt agttgccagc ggga                                   34


<210>  15
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic poly-T sequence with non-homopolymeric nucleotide


<220>
<221>  misc_feature
<222>  (1)..(5)
<223>  n is a, c, g, or t

<400>  15
nnnnnttttt twtttt                                                       16


<210>  16
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic poly-A sequence

<400>  16
aaaaaaaaaa aaaaaa                                                       16


<210>  17
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic poly-A sequence with non-homopolymeric nucleotide

<400>  17
aaaawaaaaa a                                                            11


<210>  18
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic poly-T sequence


<220>
<221>  misc_feature
<222>  (12)..(16)
<223>  n is t; may be wholly present (n = 5) or wholly absent (n = 0)

<400>  18
tttttttttt tnnnnn                                                       16


<210>  19
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic poly-A sequence

<400>  19
aaaaaaaaaa a                                                            11


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic sequence - Target RNA

<400>  20
ggtgcagaca gtggcaatca                                                   20


<210>  21
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic sequence - Target RNA

<400>  21
ggctgcagac agtggcaatc aaaaaaaaaa aaaaaaaaa                              39


<210>  22
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic RT primer

<400>  22
tttttttttt gtttttgat                                                    19


<210>  23
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic cDNA sequence

<400>  23
tttttttttt gtttttgatt gccactgtct gcacc                                  35


<210>  24
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synethetic PCR primer 1

<400>  24
tcgggtgcag acag                                                         14


<210>  25
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR primer 2

<400>  25
aggttgtttt tgattgc                                                      17


<210>  26
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR product

<400>  26
aggttgtttt tgattgccac tgtctgcacc cga                                    33


<210>  27
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR product (reverse complement)

<400>  27
tcgggtgcag acagtggcaa tcaaaaacaa cct                                    33


<210>  28
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic RT primer

<400>  28
tgattgccac                                                              10


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic cDNA sequence

<400>  29
tgattgccac tgtctgcacc                                                   20


<210>  30
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide sequence

<400>  30
tgattgccac tgtctgcagt a                                                 21


<210>  31
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic terminal transferase sequence

<400>  31
tttttttttt tttttttttg attgccactg tctgcacc                               38


<210>  32
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic RT primer

<400>  32
tttttttttt tttttgtttt tgattgccac                                        30


<210>  33
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR primer 2

<400>  33
aggttttttt tttttgattg c                                                 21


<210>  34
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR product

<400>  34
aggttttttt ttttgattgc cactgtctgc acccga                                 36


<210>  35
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic PCR product (reverse complement)

<400>  35
tcgggtgcag acagtggcaa tcaaaaaaaa aaacct                                 36


