                         SEQUENCE LISTING

<110>  DE CAMPOS ALVES VIDIGAL, Joana
       VENTURA, Andrea
 
<120>  COMPOSITION FOR CO-EXPRESSION OF RNA MOLECULES AND METHOD OF USE 
       THEREOF

<130>  228770

<150>  US 62/114,957
<151>  2015-02-11

<160>  47    

<170>  PatentIn version 3.5

<210>  1
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 110nt DNA oligo


<220>
<221>  misc_feature
<222>  (24)..(43)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (71)..(90)
<223>  n is a, c, g, or t

<400>  1
cttgtggaaa ggacgaaaca ccgnnnnnnn nnnnnnnnnn nnngtttggg tcttcgagaa       60

gacctcaccg nnnnnnnnnn nnnnnnnnnn gttttagagc tagaaatagc                 110


<210>  2
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 3' region of the U6 promoter (forward primer)

<400>  2
tttcttggct ttatatatct tgtggaaagg acgaaac                                37


<210>  3
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 5' region of the sgRNA scaffold (reverse primer)

<400>  3
gactagcctt attttaactt gctatttcta gctctaaaac                             40


<210>  4
<211>  137
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Vector insert


<220>
<221>  misc_feature
<222>  (42)..(56)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (83)..(97)
<223>  n is a, c, g, or t

<400>  4
tttcttggct ttatatatct tgtggaaagg acgaaacacc gnnnnnnnnn nnnnnntttg       60

ggtcttcgag aagacctcac cgnnnnnnnn nnnnnnngtt ttagagctag aaatagcaag      120

ttaaaataag gctagtc                                                     137


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Vector forward primer

<400>  5
ggcaagtttg tggaattggt                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Vector reverse primer

<400>  6
tctctaggca ccggttcaat                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: sgRNA1 probe

<400>  7
ttggacgccc tcgcagtggc                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: sgRNA2 probe

<400>  8
cctgttcggc acacctgctg                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: sgRNA target sites (forward primer)

<400>  9
aagttcgagg ccatctctga                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: sgRNA target sites (reverse primer)

<400>  10
caggtgaagt cgctccctac                                                   20


<210>  11
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: gRNA1

<400>  11
ggacgaaaca ccgtaagttt aacgttgata gccgttttag agctagaa                    48


<210>  12
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: gRNA2

<400>  12
ggacgaaaca ccgcctctgt agaatcgatc gaggttttag agctagaa                    48


<210>  13
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Oligo A gRNA1 DNA oligo

<400>  13
gcgacaaaac cgaaaatctg t                                                 21


<210>  14
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Oligo A gRNA2 DNA oligo

<400>  14
gatgtcttta atctacctcg a                                                 21


<210>  15
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Oligo B gRNA1 DNA oligo

<400>  15
gcacatgtca gcgttttcaa t                                                 21


<210>  16
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Oligo B gRNA2 DNA oligo

<400>  16
gttccaagta ggtagatgcg a                                                 21


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Oligo C gRNA1 DNA oligo

<400>  17
gttcagtccc aatcgagtgc                                                   20


<210>  18
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Oligo C gRNA2 DNA oligo

<400>  18
gctggtcgca ccgagatcta g                                                 21


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Oligo D gRNA1 DNA oligo

<400>  19
ggagagggga acggtatcct                                                   20


<210>  20
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Oligo D gRNA2 DNA oligo

<400>  20
gtgacaacat attcggtagt a                                                 21


<210>  21
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Oligo E gRNA1 DNA oligo

<400>  21
gagatgacag gggccatgga g                                                 21


<210>  22
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Oligo E gRNA2 DNA oligo

<400>  22
gccttctcaa aaaacttacc a                                                 21


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Oligo F gRNA1 DNA oligo

<400>  23
ggctatcaac gttaaactta                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Oligo F gRNA2 DNA oligo

<400>  24
gcttctgcct ccgcgcaaat                                                   20


<210>  25
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Oligo G gRNA1 DNA oligo

<400>  25
gccccgagcc atggccgcgt c                                                 21


<210>  26
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Oligo G gRNA2 DNA oligo

<400>  26
gggaaaagtc tccaccggac g                                                 21


<210>  27
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Oligo H gRNA1 DNA oligo

<400>  27
gctggagctt ggggacctta g                                                 21


<210>  28
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Oligo H gRNA2 DNA oligo

<400>  28
gtgtcctccc tcacaccgct a                                                 21


<210>  29
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Oligo I gRNA1 DNA oligo

<400>  29
gcctctgtag aatcgatcga g                                                 21


<210>  30
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Oligo I gRNA2 DNA oligo

<400>  30
gtaagtttaa cgttgatagc c                                                 21


<210>  31
<211>  3396
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: pDonor sequence

<400>  31
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc       60

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga      120

gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc      180

caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc      240

ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag      300

cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa      360

agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac      420

cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg      480

caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg      540

gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg      600

taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca      660

ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgatgaaga      720

cctgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa      780

agtggcaccg agtcggtgct tttttgtttt agagctagaa atagcaagtt aaaataaggc      840

tagtccgttt ttagcgcgtg cgccaattct gcagacaaat ggctctagag agggcctatt      900

tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattggaat      960

taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt     1020

tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt     1080

aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgt     1140

ttgggtcttc atcaagctta tcgataccgt cgacctcgag ggggggcccg gtacccagct     1200

tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc     1260

ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt     1320

gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc     1380

ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg     1440

ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct     1500

cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca     1560

cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga     1620

accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc     1680

acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg     1740

cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat     1800

acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt     1860

atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc     1920

agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg     1980

acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg     2040

gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg     2100

gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg     2160

gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca     2220

gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga     2280

acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga     2340

tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt     2400

ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt     2460

catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat     2520

ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag     2580

caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct     2640

ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt     2700

tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg     2760

cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca     2820

aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt     2880

tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat     2940

gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac     3000

cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa     3060

aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt     3120

tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt     3180

tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa     3240

gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt     3300

atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa     3360

taggggttcc gcgcacattt ccccgaaaag tgccac                               3396


<210>  32
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: F1

<400>  32
tttcttggct ttatatatct tgtggaaagg acgaaac                                37


<210>  33
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: R1

<400>  33
gactagcctt attttaactt gctatttcta gctctaaaac                             40


<210>  34
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: F2

<400>  34
ggcaagtttg tggaattggt                                                   20


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: R2

<400>  35
tctctaggca ccggttcaat                                                   20


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: F3

<400>  36
aagttcgagg ccatctctga                                                   20


<210>  37
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: R3

<400>  37
caggtgaagt cgctccctac                                                   20


<210>  38
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: F4

<400>  38
ccagtactct cctcccctca                                                   20


<210>  39
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: R4

<400>  39
ctcgggtggc tcataaggta                                                   20


<210>  40
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: F5

<400>  40
tttgagacta taaatatgca tgcgagaaaa gccttgtttg                             40


<210>  41
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: F6

<400>  41
tttgagactt tatatatgca tgcgagaaaa gccttgtttg                             40


<210>  42
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: mU6

<400>  42
gcaggggcca tgctaatctt ctctgtatcg                                        30


<210>  43
<211>  435
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: pDonor_hU6

<400>  43
gaagacctgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt       60

gaaaaagtgg caccgagtcg gtgctttttt gttttagagc tagaaatagc aagttaaaat      120

aaggctagtc cgtttttagc gcgtgcgcca attctgcaga caaatggctc tagagagggc      180

ctatttccca tgattccttc atatttgcat atacgataca aggctgttag agagataatt      240

ggaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa      300

taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt      360

accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac      420

accgtttggg tcttc                                                       435


<210>  44
<211>  508
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: pDonor_mU6

<400>  44
gaagacctgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt       60

gaaaaagtgg caccgagtcg gtgctttttt gttttagagc tagaaatagc aagttaaaat      120

aaggctagtc cgtttttagc gcgtgcgcca attctgcaga caaatggctc tagatctaga      180

gatccgacgc cgccatctct aggcccgcgc cggccccctc gcacagactt gtgggagaag      240

ctcggctact cccctgcccc ggttaatttg catataatat ttcctagtaa ctatagaggc      300

ttaatgtgcg ataaaagaca gataatctgt tctttttaat actagctaca ttttacatga      360

taggcttgga tttctataag agatacaaat actaaattat tattttaaaa aacagcacaa      420

aaggaaactc accctaactg taaagtaatt gtgtgttttg agactataaa tatgcatgcg      480

agaaaagcct tgtttggttt gggtcttc                                         508


<210>  45
<211>  445
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: pDonor_sU6

<400>  45
gaagacctgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt       60

gaaaaagtgg caccgagtcg gtgctttttt gttttagagc tagaaatagc aagttaaaat      120

aaggctagtc cgtttttagc gcgtgcgcca attctgcaga caaatggctc tagagagggc      180

ctatttccca tgattccttc atatttgcat ataatatttc ctagtaacta tagaggctta      240

atgtgcgata aaagacagat aatctgttct ttttaatact agctacattt tacatgatag      300

gcttggattt ctataagaga tacaaatact aaattattat tttaaaaaac agcacaaaag      360

gaaacttacc gtaacttgaa agtaattgtg tgttttgaga ctttatatat gcatgcgaga      420

aaagccttgt ttggtttggg tcttc                                            445


<210>  46
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Oligo_hU6


<220>
<221>  misc_feature
<222>  (24)..(43)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (71)..(90)
<223>  n is a, c, g, or t

<400>  46
cttgtggaaa ggacgaaaca ccgnnnnnnn nnnnnnnnnn nnngtttggg tcttcgagaa       60

gacctcaccg nnnnnnnnnn nnnnnnnnnn gttttagagc tagaaatagc                 110


<210>  47
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Oligo_s/mU6


<220>
<221>  misc_feature
<222>  (24)..(43)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (71)..(90)
<223>  n is a, c, g, or t

<400>  47
catgcgagaa aagccttgtt tggnnnnnnn nnnnnnnnnn nnngtttggg tcttcgagaa       60

gacctcaccg nnnnnnnnnn nnnnnnnnnn gttttagagc tagaaatagc                 110


