                         SEQUENCE LISTING

<110>  CODEXIS, INC.
       ALVIZO, Oscar
       AGARD, Nicholas J.
       XIE, Xinkai
       ENTWISTLE, David
       MOORE, Jeffrey C.
       KOSJEK, Birgit
 
<120>  KETOREDUCTASE POLYPEPTIDES FOR THE SYNTHESIS OF CHIRAL COMPOUNDS

<130>  CX2-148WO1

<150>  US 62/114,380
<151>  2015-02-10

<160>  246   

<170>  PatentIn version 3.5

<210>  1
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  1
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggactct gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg catccgatga agcaggctgg acgaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg cagtttccaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagta ttgaggggtt cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggctat atcaagaccc cgctggtcga tgatctggaa      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggccacat tggcgaaccg      660

aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  2
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  2

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Ala Val Ser 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Glu Gly Phe Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Tyr Ile Lys 
            180                 185                 190         


Thr Pro Leu Val Asp Asp Leu Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  3
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  3
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgttcgggat tgtaggcgac ccgactaccg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtacg aaaaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggttcacc gcacagtga                             759


<210>  4
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  4

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Phe Gly Ile Val Gly Asp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Phe Thr Ala Gln 
                245                 250         


<210>  5
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  5
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggcccg ccgactaccg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtacg aaaaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggttcacc gcacagtga                             759


<210>  6
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  6

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Pro Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Phe Thr Ala Gln 
                245                 250         


<210>  7
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  7
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgttcgggat tgtaggcgac ccgactaccg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtacg aaaaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaaaacc gcacagtga                             759


<210>  8
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  8

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Phe Gly Ile Val Gly Asp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Lys Thr Ala Gln 
                245                 250         


<210>  9
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  9
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgttcgggat tgtaggcgac ccgactaccg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtacg aaaaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  10
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  10

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Phe Gly Ile Val Gly Asp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  11
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  11
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattg gcgtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgttcgggat tgtaggcgac ccgactaccg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtacg aaaaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaaaacc gcacagtga                             759


<210>  12
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  12

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Phe Gly Ile Val Gly Asp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Lys Thr Ala Gln 
                245                 250         


<210>  13
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  13
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattg gcgtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgttcgggat tgtaggcgac ccgactaccg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggctgg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtacg aaaaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggttcacc gcacagtga                             759


<210>  14
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  14

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Phe Gly Ile Val Gly Asp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Trp Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Phe Thr Ala Gln 
                245                 250         


<210>  15
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  15
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg gcttcgggat tgtaggcgac ccgactaccg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtacg aaaaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggttcacc gcacagtga                             759


<210>  16
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  16

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Gly 
    130                 135                 140                 


Phe Gly Ile Val Gly Asp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Phe Thr Ala Gln 
                245                 250         


<210>  17
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  17
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg gctgggggat tgtaggcgac ccgactaccg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtacg aaaaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggttcacc gcacagtga                             759


<210>  18
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  18

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Gly 
    130                 135                 140                 


Trp Gly Ile Val Gly Asp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Phe Thr Ala Gln 
                245                 250         


<210>  19
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  19
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tttttgggat tgtaggctgg ccgactaccg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgtattc acagcgtacg ataaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggagaacc gcacagtaa                             759


<210>  20
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  20

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Phe Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Tyr Ser Gln 
        195                 200                 205             


Arg Thr Ile Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  21
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  21
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggcttt ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cgatgatgga tgggtatgaa      600

ggtgctgagg aaatgttttc acagcgtacg aagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  22
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  22

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Phe Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Gly Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  23
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  23
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgtattc acagcgtacg cacaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  24
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  24

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Tyr Ser Gln 
        195                 200                 205             


Arg Thr His Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  25
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  25
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  26
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  26

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  27
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  27
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggcgat ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtacg aaaaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  28
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  28

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Asp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  29
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  29
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggcgat ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtacg attaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  30
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  30

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Asp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Ile Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  31
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  31
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgttcgggat tgtaggcgat ccgactaccg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgtattc acagcgtacg ataaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggagaacc gcacagtaa                             759


<210>  32
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  32

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Phe Gly Ile Val Gly Asp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Tyr Ser Gln 
        195                 200                 205             


Arg Thr Ile Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  33
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  33
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgttcgggat tgtaggcgac ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cgatgatgga tgggtatgaa      600

ggtgctgagg aaatgtattc acagcgtacg cacaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  34
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  34

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Phe Gly Ile Val Gly Asp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Gly Tyr Glu Gly Ala Glu Glu Met Tyr Ser Gln 
        195                 200                 205             


Arg Thr His Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  35
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  35
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgttcgggat tgtaggcgac ccgactaccg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgtattc acagcgtacg aaaaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  36
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  36

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Phe Gly Ile Val Gly Asp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Tyr Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  37
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  37
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgttcgggat tgtaggcgat ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgtattc acagcgtacg tctaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  38
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  38

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Phe Gly Ile Val Gly Asp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Tyr Ser Gln 
        195                 200                 205             


Arg Thr Ser Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  39
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  39
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagatgacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  40
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  40

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Met Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  41
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  41
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  42
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  42

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  43
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  43
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtacg gaggaaccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  44
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  44

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Glu Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  45
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  45
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

aatgctgagg aaatgttttc acagcgtacg gagggtccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  46
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  46

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Asn Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Gly Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  47
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  47
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtacg gagcatccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  48
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  48

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu His Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  49
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  49
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggccaa atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  50
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  50

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Gln Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  51
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  51
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactcatg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcacactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  52
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  52

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr His Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Thr Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  53
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  53
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtacg gagcgtccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  54
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  54

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Arg Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  55
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  55
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagattacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  56
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  56

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Ile Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  57
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  57
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagtttacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  58
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  58

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Phe Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  59
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  59
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccatga tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  60
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  60

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Met Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  61
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  61
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccgata tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  62
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  62

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Asp Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  63
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  63
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acaggaaacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  64
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  64

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Glu Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  65
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  65
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtcatgagg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  66
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  66

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly His Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  67
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  67
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagtggacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  68
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  68

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Trp Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  69
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  69
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtacg gagacccgta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  70
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  70

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Arg Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  71
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  71
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccaaaa tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  72
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  72

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Lys Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  73
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  73
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccgaaa tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  74
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  74

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Glu Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  75
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  75
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgtttga tcagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  76
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  76

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Asp Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  77
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  77
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtcatgagg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  78
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  78

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly His Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  79
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  79
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccacaa tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  80
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  80

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Thr Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  81
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  81
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagacaacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  82
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  82

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Thr Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  83
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  83
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc aagtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  84
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  84

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gln Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  85
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  85
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggttttgagg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  86
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  86

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Phe Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  87
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  87
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtacg gagacccata tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  88
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  88

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr His Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  89
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  89
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acaggttacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  90
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  90

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Val Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  91
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  91
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggttatgagg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  92
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  92

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Tyr Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  93
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  93
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgaatga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  94
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  94

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Asn Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  95
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  95
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccaata tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  96
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  96

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Asn Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  97
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  97
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtacg ccaaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  98
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  98

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Pro Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  99
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  99
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgtttga tcagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  100
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  100

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Asp Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  101
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  101
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccggta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  102
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  102

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Gly Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  103
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  103
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactcatg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  104
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  104

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr His Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  105
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  105
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggttatgagg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  106
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  106

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Tyr Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  107
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  107
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagggtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  108
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  108

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Gly Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  109
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  109
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtttg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  110
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  110

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Leu Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  111
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  111
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgtttgg tcagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  112
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  112

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Gly Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  113
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  113
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctttgg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  114
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  114

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Leu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  115
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  115
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtcca gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  116
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  116

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Pro Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  117
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  117
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtacg gagggtccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  118
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  118

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Gly Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  119
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  119
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgaaaga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  120
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  120

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Lys Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  121
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  121
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccccta tgggtcacaa tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  122
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  122

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Asn Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  123
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  123
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggct tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggttatgagg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  124
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  124

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Leu Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Tyr Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  125
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  125
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgtttta tcagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  126
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  126

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Tyr Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  127
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  127
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtcgtgagg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  128
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  128

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Arg Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  129
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  129
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgttttc acagtatacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  130
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  130

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Tyr Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  131
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  131
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgcattc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  132
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  132

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met His Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  133
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  133
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgacttgcg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcctt atcaagaccc cgatgattga tgattatgaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  134
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  134

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Cys Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Leu Ile Lys 
            180                 185                 190         


Thr Pro Met Ile Asp Asp Tyr Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  135
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  135
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgctgagg aaatgtggtc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  136
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  136

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Glu Glu Met Trp Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  137
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  137
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc cggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgattatgaa      600

ggtgcttggg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  138
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  138

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Pro Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Tyr Glu Gly Ala Trp Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  139
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  139
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcctt atcaagaccc cgatgaatga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccccta tgggtcacca cggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  140
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  140

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Leu Ile Lys 
            180                 185                 190         


Thr Pro Met Asn Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His His Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  141
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  141
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacgacacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggcgat ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  142
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  142

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Asp Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Asp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  143
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  143
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcacg atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaatccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  144
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  144

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Thr Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Asn Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  145
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  145
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaatccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggaca gcatggtaa                             759


<210>  146
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  146

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Asn Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Trp 
                245                 250         


<210>  147
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  147
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggccct atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttttc acagggaacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  148
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  148

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Gly Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  149
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  149
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaagaa ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactactg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gagacaccta tgggtcacat cggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  150
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  150

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Glu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  151
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  151
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgaacga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  152
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  152

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Asn Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  153
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  153
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gaggcaccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggggc gcacagtaa                             759


<210>  154
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  154

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Ala Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Gly Ala Gln 
                245                 250         


<210>  155
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  155
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaagcgat ccgactacag gggcatactg tgcgtccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgacgaagaa      600

ggtgcttggg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  156
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  156

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Ser Asp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Trp Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  157
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  157
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgta tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggaca gcatggtaa                             759


<210>  158
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  158

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Tyr Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Trp 
                245                 250         


<210>  159
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  159
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gaggcaccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggaca gcatggtaa                             759


<210>  160
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  160

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Ala Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Trp 
                245                 250         


<210>  161
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  161
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgttg gtctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgcatga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggaca gcacagtaa                             759


<210>  162
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  162

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Gly Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met His Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  163
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  163
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggcgat ccgactacag gggcatactg tgcgtccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgacgaagaa      600

ggtgctgggg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  164
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  164

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Asp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Gly Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  165
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  165
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaattg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactactg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgcagga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  166
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  166

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Gln Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  167
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  167
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tttgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgcatga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gaggcaccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggaca gcacagtaa                             759


<210>  168
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  168

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met His Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Ala Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  169
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  169
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gagccaccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  170
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  170

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Pro Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  171
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  171
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc aagtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gaacacccga tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  172
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  172

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gln Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu His Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  173
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  173
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaagcgat ccgactacag gggcatactg tgcgtccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  174
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  174

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Ser Asp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  175
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  175
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tttgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaatccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggggc gcacagtaa                             759


<210>  176
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  176

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Asn Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Gly Ala Gln 
                245                 250         


<210>  177
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  177
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgcatta tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  178
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  178

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met His Tyr Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  179
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  179
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgta tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggggc gcatggtaa                             759


<210>  180
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  180

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Tyr Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Gly Ala Trp 
                245                 250         


<210>  181
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  181
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tttgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gaggcaccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggggc gcatggtaa                             759


<210>  182
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  182

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Ala Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Gly Ala Trp 
                245                 250         


<210>  183
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  183
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaacatttgt tcagcgtacg gaggcaccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggaca gcatggtaa                             759


<210>  184
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  184

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Thr Phe Val Gln 
        195                 200                 205             


Arg Thr Glu Ala Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Trp 
                245                 250         


<210>  185
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  185
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggattc aagtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactaccg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gaaactggta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  186
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  186

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gln Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Gly Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  187
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  187
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaagaa ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactactg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gaggtcccta tgggtcacat cggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  188
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  188

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Glu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Val Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  189
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  189
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggcgat ccgactacag gggcatactg tgtgtccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  190
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  190

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Asp Pro Thr Thr Gly Ala Tyr Cys Val Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  191
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  191
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggcgat ccgactacag gggcatactg tgtgtccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga ttcggaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  192
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  192

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Asp Pro Thr Thr Gly Ala Tyr Cys Val Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Ser Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  193
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  193
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactcccg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggccca atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gagcccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  194
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  194

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Pro Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Pro Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  195
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  195
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggcgat ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga ttcggaagaa      600

ggtgcttggg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  196
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  196

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Asp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Ser Glu Glu Gly Ala Trp Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  197
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  197
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaattg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactactg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gagacaatga tgggtcacat cggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  198
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  198

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Met Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  199
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  199
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggcgat ccgactacag gggcatactg tgcgtccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga ttcggaagaa      600

ggtgctttgg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  200
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  200

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Asp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Ser Glu Glu Gly Ala Leu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  201
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  201
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggcgat ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgacgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  202
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  202

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Asp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  203
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  203
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaattg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactactg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gaggtcaata tgggtcacat cggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  204
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  204

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Val Asn Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  205
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  205
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactaccg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gaacgcccga tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  206
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  206

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Arg Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  207
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  207
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaattg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactactg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gagacagaga tgggtcacat cggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  208
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  208

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Glu Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  209
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  209
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgcatga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  210
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  210

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met His Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  211
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  211
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaattg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactactg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gagacaacga tgggtcacat cggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gccgaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  212
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  212

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Thr Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  213
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  213
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactaccg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gaaactggta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  214
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  214

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Gly Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  215
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  215
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gaagaaggta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  216
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  216

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Glu Gly Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  217
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  217
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaatgg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactactg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gagacaaata tgggtcacat cggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  218
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  218

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Trp Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Asn Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  219
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  219
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaattg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactactg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gagacaaata tgggtcacat cggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  220
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  220

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Asn Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  221
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  221
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaattg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttctc ccagcgtacg gagggcaata tgggtcacat cggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  222
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  222

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Gly Asn Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  223
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  223
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gaacacaaga tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  224
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  224

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu His Lys Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  225
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  225
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactaccg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gaaactaaga tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  226
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  226

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Lys Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  227
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  227
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactaccg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gaacgcggta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  228
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  228

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Arg Gly Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  229
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  229
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agttggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  230
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  230

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Val Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  231
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  231
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgaaagctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  232
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  232

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Lys Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  233
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  233
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctacag aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  234
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  234

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Thr Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  235
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  235
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctcaag aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggaggacc gcacagtaa                             759


<210>  236
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  236

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Gln Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Arg Thr Ala Gln 
                245                 250         


<210>  237
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  237
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca aggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgt      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tatccgatga agcaggctgg cctaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt agtgaacaat gcagggatta tggtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg tgtgggggat tgtaggctgg ccgactacag gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctgcttt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccg atcaagaccc cgatgatgga tgatgaagaa      600

ggtgctgagg aaatgttttc acagcgtacg gagaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cggggggacc gcacagtaa                             759


<210>  238
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldo-keto reductase variant from Lactobacillus kefiri

<400>  238

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Met Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Trp Gly Ile Val Gly Trp Pro Thr Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Leu Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Met Met Asp Asp Glu Glu Gly Ala Glu Glu Met Phe Ser Gln 
        195                 200                 205             


Arg Thr Glu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Gly Thr Ala Gln 
                245                 250         


<210>  239
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic engineered aldo-keto reductase variant


<220>
<221>  MISC_FEATURE
<222>  (17)..(17)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (40)..(40)
<223>  Xaa is a basic residue

<220>
<221>  MISC_FEATURE
<222>  (64)..(64)
<223>  Xaa is an aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (68)..(68)
<223>  Xaa is a non-polar or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (71)..(71)
<223>  Xaa is a non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (94)..(94)
<223>  Xaa is a polar, aliphatic, or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (96)..(96)
<223>  Xaa is an aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (102)..(102)
<223>  Xaa is an acidic or polar residue

<220>
<221>  MISC_FEATURE
<222>  (110)..(110)
<223>  Xaa is an acidic or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (114)..(114)
<223>  Xaa is a non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (135)..(135)
<223>  Xaa is a basic residue

<220>
<221>  MISC_FEATURE
<222>  (144)..(144)
<223>  Xaa is a non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (145)..(145)
<223>  Xaa is an aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (147)..(147)
<223>  Xaa is a non-polar or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (149)..(149)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (150)..(150)
<223>  Xaa is an aromatic or acidic residue

<220>
<221>  MISC_FEATURE
<222>  (153)..(153)
<223>  Xaa is a polar or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (157)..(157)
<223>  Xaa is a non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (158)..(158)
<223>  Xaa is a non-polar or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (173)..(173)
<223>  Xaa is a non-polar or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (175)..(175)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (190)..(190)
<223>  Xaa is a polar, aliphatic, aromatic, or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (195)..(195)
<223>  Xaa is a non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (196)..(196)
<223>  Xaa is a polar, basic, non-polar, aromatic, or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (197)..(197)
<223>  Xaa is an aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (198)..(198)
<223>  Xaa is a polar or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (199)..(199)
<223>  Xaa is an acidic residue

<220>
<221>  MISC_FEATURE
<222>  (201)..(201)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (202)..(202)
<223>  Xaa is a aromatic or basic residue

<220>
<221>  MISC_FEATURE
<222>  (203)..(203)
<223>  Xaa is a non-polar, aromatic, aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (205)..(205)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (206)..(206)
<223>  Xaa is an aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (207)..(207)
<223>  Xaa is a non-polar, aromatic, acidic, or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (209)..(209)
<223>  Xaa is an aromatic, acidic, non-polar, polar, or aliphatic 
       residue

<220>
<221>  MISC_FEATURE
<222>  (210)..(210)
<223>  Xaa is a non-polar or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (211)..(211)
<223>  Xaa is an aromatic, acidic, aliphatic, polar, or non-polar 
       residue

<220>
<221>  MISC_FEATURE
<222>  (212)..(212)
<223>  Xaa is a non-polar, aliphatic, acidic, basic, or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (213)..(213)
<223>  Xaa is an aromatic, basic, acidic, polar, non-polar, or aliphatic
       residue

<220>
<221>  MISC_FEATURE
<222>  (217)..(217)
<223>  Xaa is a polar or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (226)..(226)
<223>  Xaa is an aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (233)..(233)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (249)..(249)
<223>  Xaa is a non-polar or basic residue

<220>
<221>  MISC_FEATURE
<222>  (250)..(250)
<223>  Xaa is a non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (252)..(252)
<223>  Xaa is an aromatic residue

<400>  239

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Xaa Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Xaa Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Xaa 
    50                  55                  60                  


Ser Asp Glu Xaa Gly Trp Xaa Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Xaa Val Xaa 
                85                  90                  95      


Lys Ser Val Glu Asp Xaa Thr Thr Glu Glu Trp Arg Lys Xaa Leu Ser 
            100                 105                 110         


Val Xaa Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Xaa Ala Ser Ile Ile Asn Met Ser Ser Xaa 
    130                 135                 140                 


Xaa Gly Xaa Val Xaa Xaa Pro Thr Xaa Gly Ala Tyr Xaa Xaa Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Xaa Cys Xaa Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Xaa Ile Lys 
            180                 185                 190         


Thr Pro Xaa Xaa Xaa Xaa Xaa Glu Xaa Xaa Xaa Glu Xaa Xaa Xaa Gln 
        195                 200                 205             


Xaa Xaa Xaa Xaa Xaa Met Gly His Xaa Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Xaa Cys Val Tyr Leu Ala Ser Xaa Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Xaa Xaa Ala Xaa 
                245                 250         


<210>  240
<211>  252
<212>  PRT
<213>  Artificial Sequences

<220>
<223>  Synthetic engineered aldo-keto reductase variant


<220>
<221>  MISC_FEATURE
<222>  (17)..(17)
<223>  Xaa is a polar or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (40)..(40)
<223>  Xaa is a basic or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (64)..(64)
<223>  Xaa is an aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (68)..(68)
<223>  Xaa is a non-polar or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (71)..(71)
<223>  Xaa is a non-polar or polar residue

<220>
<221>  MISC_FEATURE
<222>  (94)..(94)
<223>  Xaa is a polar, aliphatic, or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (96)..(96)
<223>  Xaa is an aliphatic or polar residue

<220>
<221>  MISC_FEATURE
<222>  (102)..(102)
<223>  Xaa is an acidic or polar residue

<220>
<221>  MISC_FEATURE
<222>  (110)..(110)
<223>  Xaa is an aliphatic, acidic, or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (114)..(114)
<223>  Xaa is a polar or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (135)..(135)
<223>  Xaa is a non-polar or basic residue

<220>
<221>  MISC_FEATURE
<222>  (144)..(144)
<223>  Xaa is an aliphatic or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (145)..(145)
<223>  Xaa is an acidic or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (147)..(147)
<223>  Xaa is an aromatic, non-polar, or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (149)..(149)
<223>  Xaa is a non-polar or polar residue

<220>
<221>  MISC_FEATURE
<222>  (150)..(150)
<223>  Xaa is an aromatic or acidic residue

<220>
<221>  MISC_FEATURE
<222>  (153)..(153)
<223>  Xaa is an aliphatic, polar or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (157)..(157)
<223>  Xaa is a non-polar or polar residue

<220>
<221>  MISC_FEATURE
<222>  (158)..(158)
<223>  Xaa is a non-polar or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (173)..(173)
<223>  Xaa is an acidic, non-polar, or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (175)..(175)
<223>  Xaa is an aliphatic or polar residue

<220>
<221>  MISC_FEATURE
<222>  (190)..(190)
<223>  Xaa is a polar, aliphatic, aromatic, or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (195)..(195)
<223>  Xaa is a non-polar or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (196)..(196)
<223>  Xaa is a polar, basic, non-polar, aromatic, or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (197)..(197)
<223>  Xaa is an acidic or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (198)..(198)
<223>  Xaa is an acidic, polar, or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (199)..(199)
<223>  Xaa is an aliphatic or acidic residue

<220>
<221>  MISC_FEATURE
<222>  (201)..(201)
<223>  Xaa is a non-polar or polar residue

<220>
<221>  MISC_FEATURE
<222>  (202)..(202)
<223>  Xaa is an aliphatic, aromatic, or basic residue

<220>
<221>  MISC_FEATURE
<222>  (203)..(203)
<223>  Xaa is an acidic, non-polar, aromatic, or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (205)..(205)
<223>  Xaa is a non-polar or polar residue

<220>
<221>  MISC_FEATURE
<222>  (206)..(206)
<223>  Xaa is a non-polar or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (207)..(207)
<223>  Xaa is a polar, non-polar, aromatic, acidic, or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (209)..(209)
<223>  Xaa is a basic, aromatic, acidic, non-polar, polar, or aliphatic 
       residue

<220>
<221>  MISC_FEATURE
<222>  (210)..(210)
<223>  Xaa is a polar, non-polar, or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (211)..(211)
<223>  Xaa is a basic, aromatic, acidic, aliphatic, polar, or non-polar 
       residue

<220>
<221>  MISC_FEATURE
<222>  (212)..(212)
<223>  Xaa is a polar, non-polar, aliphatic, acidic, basic, or aromatic 
       residue

<220>
<221>  MISC_FEATURE
<222>  (213)..(213)
<223>  Xaa is an aromatic, basic, acidic, polar, non-polar, or aliphatic
       residue

<220>
<221>  MISC_FEATURE
<222>  (217)..(217)
<223>  Xaa is an aliphatic, polar, or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (226)..(226)
<223>  Xaa is an aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (233)..(233)
<223>  Xaa is an acidic or polar residue

<220>
<221>  MISC_FEATURE
<222>  (249)..(249)
<223>  Xaa is an aromatic, non-polar, or basic residue

<220>
<221>  MISC_FEATURE
<222>  (250)..(250)
<223>  Xaa is a polar or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (252)..(252)
<223>  Xaa is a polar or aromatic residue

<400>  240

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Xaa Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Xaa Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Xaa 
    50                  55                  60                  


Ser Asp Glu Xaa Gly Trp Xaa Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Xaa Val Xaa 
                85                  90                  95      


Lys Ser Val Glu Asp Xaa Thr Thr Glu Glu Trp Arg Lys Xaa Leu Ser 
            100                 105                 110         


Val Xaa Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Xaa Ala Ser Ile Ile Asn Met Ser Ser Xaa 
    130                 135                 140                 


Xaa Gly Xaa Val Xaa Xaa Pro Thr Xaa Gly Ala Tyr Xaa Xaa Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Xaa Cys Xaa Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Xaa Ile Lys 
            180                 185                 190         


Thr Pro Xaa Xaa Xaa Xaa Xaa Glu Xaa Xaa Xaa Glu Xaa Xaa Xaa Gln 
        195                 200                 205             


Xaa Xaa Xaa Xaa Xaa Met Gly His Xaa Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Xaa Cys Val Tyr Leu Ala Ser Xaa Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Xaa Xaa Ala Xaa 
                245                 250         


<210>  241
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic engineered aldo-keto reductase variant


<220>
<221>  MISC_FEATURE
<222>  (17)..(17)
<223>  Xaa is a polar or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (40)..(40)
<223>  Xaa is a basic or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (64)..(64)
<223>  Xaa is an aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (68)..(68)
<223>  Xaa is a non-polar or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (71)..(71)
<223>  Xaa is a non-polar or polar residue

<220>
<221>  MISC_FEATURE
<222>  (94)..(94)
<223>  Xaa is a polar, aliphatic, or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (96)..(96)
<223>  Xaa is an aliphatic or polar residue

<220>
<221>  MISC_FEATURE
<222>  (102)..(102)
<223>  Xaa is an acidic or polar residue

<220>
<221>  MISC_FEATURE
<222>  (110)..(110)
<223>  Xaa is an aliphatic, acidic, or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (114)..(114)
<223>  Xaa is a polar or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (135)..(135)
<223>  Xaa is a non-polar or basic residue

<220>
<221>  MISC_FEATURE
<222>  (144)..(144)
<223>  Xaa is an aliphatic or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (145)..(145)
<223>  Xaa is an acidic or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (147)..(147)
<223>  Xaa is an aromatic, non-polar, or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (149)..(149)
<223>  Xaa is a non-polar or polar residue

<220>
<221>  MISC_FEATURE
<222>  (150)..(150)
<223>  Xaa is an aromatic or acidic residue

<220>
<221>  MISC_FEATURE
<222>  (153)..(153)
<223>  Xaa is an aliphatic, polar, or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (157)..(157)
<223>  Xaa is a non-polar or polar residue

<220>
<221>  MISC_FEATURE
<222>  (158)..(158)
<223>  Xaa is a non-polar or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (175)..(175)
<223>  Xaa is an aliphatic or polar residue

<220>
<221>  MISC_FEATURE
<222>  (190)..(190)
<223>  Xaa is a polar, aliphatic, aromatic, or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (195)..(195)
<223>  Xaa is a non-polar or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (196)..(196)
<223>  Xaa is a polar, basic, non-polar, aromatic, or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (197)..(197)
<223>  Xaa is an acidic or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (198)..(198)
<223>  Xaa is an acidic, polar, or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (199)..(199)
<223>  Xaa is an aliphatic or acidic residue

<220>
<221>  MISC_FEATURE
<222>  (201)..(201)
<223>  Xaa is a non-polar or polar residue

<220>
<221>  MISC_FEATURE
<222>  (202)..(202)
<223>  Xaa is an aliphatic, aromatic, or basic residue

<220>
<221>  MISC_FEATURE
<222>  (203)..(203)
<223>  Xaa is an acidic, non-polar, aromatic, or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (205)..(205)
<223>  Xaa is a non-polar or polar residue

<220>
<221>  MISC_FEATURE
<222>  (206)..(206)
<223>  Xaa is a non-polar or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (207)..(207)
<223>  Xaa is a polar, non-polar, aromatic, acidic, or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (209)..(209)
<223>  Xaa is a basic, aromatic, acidic, non-polar, polar, or aliphatic 
       residue

<220>
<221>  MISC_FEATURE
<222>  (210)..(210)
<223>  Xaa is a polar, non-polar, or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (211)..(211)
<223>  Xaa is a basic, aromatic, acidic, aliphatic, polar, or non-polar 
       residue

<220>
<221>  MISC_FEATURE
<222>  (212)..(212)
<223>  Xaa is a polar, non-polar, aliphatic, acidic, basic, or aromatic 
       residue

<220>
<221>  MISC_FEATURE
<222>  (213)..(213)
<223>  Xaa is an aromatic, basic, acidic, polar, non-polar, or aliphatic
       residue

<220>
<221>  MISC_FEATURE
<222>  (217)..(217)
<223>  Xaa is an aliphatic, polar, or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (226)..(226)
<223>  Xaa is an aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (233)..(233)
<223>  Xaa is an acidic or polar residue

<220>
<221>  MISC_FEATURE
<222>  (249)..(249)
<223>  Xaa is an aromatic, non-polar, or basic residue

<220>
<221>  MISC_FEATURE
<222>  (250)..(250)
<223>  Xaa is a polar or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (252)..(252)
<223>  Xaa is a polar or aromatic residue

<400>  241

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Xaa Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Xaa Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Xaa 
    50                  55                  60                  


Ser Asp Glu Xaa Gly Trp Xaa Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Xaa Val Xaa 
                85                  90                  95      


Lys Ser Val Glu Asp Xaa Thr Thr Glu Glu Trp Arg Lys Xaa Leu Ser 
            100                 105                 110         


Val Xaa Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Xaa Ala Ser Ile Ile Asn Met Ser Ser Xaa 
    130                 135                 140                 


Xaa Gly Xaa Val Xaa Xaa Pro Thr Xaa Gly Ala Tyr Xaa Xaa Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Leu Cys Xaa Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Xaa Ile Lys 
            180                 185                 190         


Thr Pro Xaa Xaa Xaa Xaa Xaa Glu Xaa Xaa Xaa Glu Xaa Xaa Xaa Gln 
        195                 200                 205             


Xaa Xaa Xaa Xaa Xaa Met Gly His Xaa Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Xaa Cys Val Tyr Leu Ala Ser Xaa Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Xaa Xaa Ala Xaa 
                245                 250         


<210>  242
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic engineered aldo-reductase variant


<220>
<221>  MISC_FEATURE
<222>  (17)..(17)
<223>  Xaa is a polar or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (40)..(40)
<223>  Xaa is a basic or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (64)..(64)
<223>  Xaa is an aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (68)..(68)
<223>  Xaa is a non-polar or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (71)..(71)
<223>  Xaa is a non-polar or polar residue

<220>
<221>  MISC_FEATURE
<222>  (94)..(94)
<223>  Xaa is a polar, aliphatic, or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (96)..(96)
<223>  Xaa is an aliphatic or polar residue

<220>
<221>  MISC_FEATURE
<222>  (102)..(102)
<223>  Xaa is an acidic or polar residue

<220>
<221>  MISC_FEATURE
<222>  (110)..(110)
<223>  Xaa is an aliphatic, acidic, or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (114)..(114)
<223>  Xaa is a polar or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (135)..(135)
<223>  Xaa is a non-polar or basic residue

<220>
<221>  MISC_FEATURE
<222>  (144)..(144)
<223>  Xaa is an aliphatic or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (145)..(145)
<223>  Xaa is an acidic or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (147)..(147)
<223>  Xaa is an aromatic, non-polar, or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (149)..(149)
<223>  Xaa is a non-polar or polar residue

<220>
<221>  MISC_FEATURE
<222>  (150)..(150)
<223>  Xaa is an aromatic or acidic residue

<220>
<221>  MISC_FEATURE
<222>  (153)..(153)
<223>  Xaa is an aliphatic, polar or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (157)..(157)
<223>  Xaa is a non-polar or polar residue

<220>
<221>  MISC_FEATURE
<222>  (158)..(158)
<223>  Xaa is a non-polar or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (173)..(173)
<223>  Xaa is an acidic, non-polar, or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (175)..(175)
<223>  Xaa is an aliphatic or polar residue

<220>
<221>  MISC_FEATURE
<222>  (190)..(190)
<223>  Xaa is a polar, aliphatic, aromatic, or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (195)..(195)
<223>  Xaa is a non-polar or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (196)..(196)
<223>  Xaa is a polar, basic, non-polar, aromatic, or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (197)..(197)
<223>  Xaa is an acidic or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (198)..(198)
<223>  Xaa is an acidic, polar, or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (199)..(199)
<223>  Xaa is an aliphatic or acidic residue

<220>
<221>  MISC_FEATURE
<222>  (201)..(201)
<223>  Xaa is a non-polar or polar residue

<220>
<221>  MISC_FEATURE
<222>  (202)..(202)
<223>  Xaa is an aliphatic, aromatic, or basic residue

<220>
<221>  MISC_FEATURE
<222>  (203)..(203)
<223>  Xaa is an acidic, non-polar, aromatic, or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (205)..(205)
<223>  Xaa is a non-polar or polar residue

<220>
<221>  MISC_FEATURE
<222>  (206)..(206)
<223>  Xaa is a non-polar or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (207)..(207)
<223>  Xaa is a polar, non-polar, aromatic, acidic, or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (209)..(209)
<223>  Xaa is a basic, aromatic, acidic, non-polar, polar, or aliphatic 
       residue

<220>
<221>  MISC_FEATURE
<222>  (210)..(210)
<223>  Xaa is a polar, non-polar, or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (211)..(211)
<223>  Xaa is a basic, aromatic, acidic, aliphatic, polar, or non-polar 
       residue

<220>
<221>  MISC_FEATURE
<222>  (212)..(212)
<223>  Xaa is a polar, non-polar, aliphatic, acidic, basic, or aromatic 
       residue

<220>
<221>  MISC_FEATURE
<222>  (213)..(213)
<223>  Xaa is an aromatic, basic, acidic, polar, non-polar, or aliphatic
       residue

<220>
<221>  MISC_FEATURE
<222>  (217)..(217)
<223>  Xaa is an aliphatic, polar, or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (226)..(226)
<223>  Xaa is an aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (233)..(233)
<223>  Xaa is an acidic or polar residue

<220>
<221>  MISC_FEATURE
<222>  (250)..(250)
<223>  Xaa is a polar or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (252)..(252)
<223>  Xaa is a polar or aromatic residue

<400>  242

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Xaa Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Xaa Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Xaa 
    50                  55                  60                  


Ser Asp Glu Xaa Gly Trp Xaa Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Xaa Val Xaa 
                85                  90                  95      


Lys Ser Val Glu Asp Xaa Thr Thr Glu Glu Trp Arg Lys Xaa Leu Ser 
            100                 105                 110         


Val Xaa Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Xaa Ala Ser Ile Ile Asn Met Ser Ser Xaa 
    130                 135                 140                 


Xaa Gly Xaa Val Xaa Xaa Pro Thr Xaa Gly Ala Tyr Xaa Xaa Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Xaa Cys Xaa Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Xaa Ile Lys 
            180                 185                 190         


Thr Pro Xaa Xaa Xaa Xaa Xaa Glu Xaa Xaa Xaa Glu Xaa Xaa Xaa Gln 
        195                 200                 205             


Xaa Xaa Xaa Xaa Xaa Met Gly His Xaa Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Xaa Cys Val Tyr Leu Ala Ser Xaa Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Gly Xaa Ala Xaa 
                245                 250         


<210>  243
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic engineered aldo-keto reductase variant


<220>
<221>  MISC_FEATURE
<222>  (17)..(17)
<223>  Xaa is a polar or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (40)..(40)
<223>  Xaa is a basic or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (64)..(64)
<223>  Xaa is an aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (68)..(68)
<223>  Xaa is a non-polar or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (71)..(71)
<223>  Xaa is a non-polar or polar residue

<220>
<221>  MISC_FEATURE
<222>  (94)..(94)
<223>  Xaa is a polar, aliphatic, or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (96)..(96)
<223>  Xaa is an aliphatic or polar residue

<220>
<221>  MISC_FEATURE
<222>  (102)..(102)
<223>  Xaa is an acidic or polar residue

<220>
<221>  MISC_FEATURE
<222>  (110)..(110)
<223>  Xaa is an aliphatic, acidic, or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (114)..(114)
<223>  Xaa is a polar or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (135)..(135)
<223>  Xaa is a non-polar or basic residue

<220>
<221>  MISC_FEATURE
<222>  (144)..(144)
<223>  Xaa is an aliphatic or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (145)..(145)
<223>  Xaa is an acidic  or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (147)..(147)
<223>  Xaa is an aromatic, non-polar, or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (149)..(149)
<223>  Xaa is a non-polar or polar residue

<220>
<221>  MISC_FEATURE
<222>  (150)..(150)
<223>  Xaa is an aromatic or acidic residue

<220>
<221>  MISC_FEATURE
<222>  (153)..(153)
<223>  Xaa is an aliphatic, polar, or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (157)..(157)
<223>  Xaa is a non-polar or polar residue

<220>
<221>  MISC_FEATURE
<222>  (158)..(158)
<223>  Xaa is a non-polar or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (175)..(175)
<223>  Xaa is an aliphatic or polar residue

<220>
<221>  MISC_FEATURE
<222>  (190)..(190)
<223>  Xaa is a polar, aliphatic, aromatic, or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (195)..(195)
<223>  Xaa is a non-polar or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (196)..(196)
<223>  Xaa is a polar, basic, non-polar, aromatic, or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (197)..(197)
<223>  Xaa is an acidic or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (198)..(198)
<223>  Xaa is an acidic, polar, or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (199)..(199)
<223>  Xaa is an aliphatic or acidic residue

<220>
<221>  MISC_FEATURE
<222>  (201)..(201)
<223>  Xaa is a non-polar or polar residue

<220>
<221>  MISC_FEATURE
<222>  (202)..(202)
<223>  Xaa is an aliphatic, aromatic, or basic residue

<220>
<221>  MISC_FEATURE
<222>  (203)..(203)
<223>  Xaa is an acidic, non-polar, aromatic, or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (205)..(205)
<223>  Xaa is a non-polar or polar residue

<220>
<221>  MISC_FEATURE
<222>  (206)..(206)
<223>  Xaa is a non-polar or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (207)..(207)
<223>  Xaa is a polar, non-polar, aromatic, acidic, or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (209)..(209)
<223>  Xaa is a basic, aromatic, acidic, non-polar, polar, or aliphatic 
       residue

<220>
<221>  MISC_FEATURE
<222>  (210)..(210)
<223>  Xaa is a polar, non-polar, or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (211)..(211)
<223>  Xaa is a basic, aromatic, acidic, aliphatic, polar, or non-polar 
       residue

<220>
<221>  MISC_FEATURE
<222>  (212)..(212)
<223>  Xaa is a polar, non-polar, aliphatic, acidic, basic, or aromatic 
       residue

<220>
<221>  MISC_FEATURE
<222>  (213)..(213)
<223>  Xaa is an aromatic, basic, acidic, polar, non-polar, or aliphatic
       residue

<220>
<221>  MISC_FEATURE
<222>  (217)..(217)
<223>  Xaa is an aliphatic, polar, or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (226)..(226)
<223>  Xaa is an aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (233)..(233)
<223>  Xaa is an acidic or polar residue

<220>
<221>  MISC_FEATURE
<222>  (250)..(250)
<223>  Xaa is a polar or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (252)..(252)
<223>  Xaa is a polar or aromatic residue

<400>  243

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Xaa Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Xaa Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Xaa 
    50                  55                  60                  


Ser Asp Glu Xaa Gly Trp Xaa Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Xaa Val Xaa 
                85                  90                  95      


Lys Ser Val Glu Asp Xaa Thr Thr Glu Glu Trp Arg Lys Xaa Leu Ser 
            100                 105                 110         


Val Xaa Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Xaa Ala Ser Ile Ile Asn Met Ser Ser Xaa 
    130                 135                 140                 


Xaa Gly Xaa Val Xaa Xaa Pro Thr Xaa Gly Ala Tyr Xaa Xaa Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Leu Cys Xaa Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Xaa Ile Lys 
            180                 185                 190         


Thr Pro Xaa Xaa Xaa Xaa Xaa Glu Xaa Xaa Xaa Glu Xaa Xaa Xaa Gln 
        195                 200                 205             


Xaa Xaa Xaa Xaa Xaa Met Gly His Xaa Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Xaa Cys Val Tyr Leu Ala Ser Xaa Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Gly Xaa Ala Xaa 
                245                 250         


<210>  244
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic engineered aldo-keto reductase variant


<220>
<221>  MISC_FEATURE
<222>  (17)..(17)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (40)..(40)
<223>  Xaa is a basic residue

<220>
<221>  MISC_FEATURE
<222>  (64)..(64)
<223>  Xaa is an aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (68)..(68)
<223>  Xaa is a non-polar or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (71)..(71)
<223>  Xaa is a non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (94)..(94)
<223>  Xaa is a polar, aliphatic, or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (96)..(96)
<223>  Xaa is an aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (102)..(102)
<223>  Xaa is an acidic or polar residue

<220>
<221>  MISC_FEATURE
<222>  (110)..(110)
<223>  Xaa is an acidic or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (114)..(114)
<223>  Xaa is a non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (135)..(135)
<223>  Xaa is a basic residue

<220>
<221>  MISC_FEATURE
<222>  (144)..(144)
<223>  Xaa is a non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (145)..(145)
<223>  Xaa is an aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (147)..(147)
<223>  Xaa is a non-polar or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (149)..(149)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (150)..(150)
<223>  Xaa is an aromatic or acidic residue

<220>
<221>  MISC_FEATURE
<222>  (153)..(153)
<223>  Xaa is a polar or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (157)..(157)
<223>  Xaa is a non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (158)..(158)
<223>  Xaa is a non-polar or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (175)..(175)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (190)..(190)
<223>  Xaa is a polar, aliphatic, aromatic, or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (195)..(195)
<223>  Xaa is a non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (196)..(196)
<223>  Xaa is a polar, basic, non-polar, aromatic, or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (197)..(197)
<223>  Xaa is an aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (198)..(198)
<223>  Xaa is a polar or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (199)..(199)
<223>  Xaa is an acidic residue

<220>
<221>  MISC_FEATURE
<222>  (201)..(201)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (202)..(202)
<223>  Xaa is an aromatic or basic residue

<220>
<221>  MISC_FEATURE
<222>  (203)..(203)
<223>  Xaa is a non-polar, aromatic, or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (205)..(205)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (206)..(206)
<223>  Xaa is an aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (207)..(207)
<223>  Xaa is a non-polar, aromatic, acidic, or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (209)..(209)
<223>  Xaa is an aromatic, acidic, non-polar, polar, or aliphatic 
       residue

<220>
<221>  MISC_FEATURE
<222>  (210)..(210)
<223>  Xaa is a non-polar or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (211)..(211)
<223>  Xaa is an aromatic, acidic, aliphatic, polar, or non-polar 
       residue

<220>
<221>  MISC_FEATURE
<222>  (212)..(212)
<223>  Xaa is a non-polar, aliphatic, acidic, basic, or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (213)..(213)
<223>  Xaa is an aromatic, basic, acidic, polar, non-polar, or aliphatic
       residue

<220>
<221>  MISC_FEATURE
<222>  (217)..(217)
<223>  Xaa is a polar or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (226)..(226)
<223>  Xaa is an aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (233)..(233)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (249)..(249)
<223>  Xaa is a non-polar or basic residue

<220>
<221>  MISC_FEATURE
<222>  (250)..(250)
<223>  Xaa is a non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (252)..(252)
<223>  Xaa is an aromatic residue

<400>  244

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Xaa Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Xaa Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Xaa 
    50                  55                  60                  


Ser Asp Glu Xaa Gly Trp Xaa Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Xaa Val Xaa 
                85                  90                  95      


Lys Ser Val Glu Asp Xaa Thr Thr Glu Glu Trp Arg Lys Xaa Leu Ser 
            100                 105                 110         


Val Xaa Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Xaa Ala Ser Ile Ile Asn Met Ser Ser Xaa 
    130                 135                 140                 


Xaa Gly Xaa Val Xaa Xaa Pro Thr Xaa Gly Ala Tyr Xaa Xaa Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Leu Cys Xaa Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Xaa Ile Lys 
            180                 185                 190         


Thr Pro Xaa Xaa Xaa Xaa Xaa Glu Xaa Xaa Xaa Glu Xaa Xaa Xaa Gln 
        195                 200                 205             


Xaa Xaa Xaa Xaa Xaa Met Gly His Xaa Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Xaa Cys Val Tyr Leu Ala Ser Xaa Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Xaa Xaa Ala Xaa 
                245                 250         


<210>  245
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic engineered aldo-keto reductase variant


<220>
<221>  MISC_FEATURE
<222>  (17)..(17)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (40)..(40)
<223>  Xaa is a basic residue

<220>
<221>  MISC_FEATURE
<222>  (64)..(64)
<223>  Xaa is an aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (68)..(68)
<223>  Xaa is a non-polar or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (71)..(71)
<223>  Xaa is a non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (94)..(94)
<223>  Xaa is a polar, aliphatic, or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (96)..(96)
<223>  Xaa is an aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (102)..(102)
<223>  Xaa is an acidic or polar residue

<220>
<221>  MISC_FEATURE
<222>  (110)..(110)
<223>  Xaa is an acidic or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (114)..(114)
<223>  Xaa is a non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (135)..(135)
<223>  Xaa is a basic residue

<220>
<221>  MISC_FEATURE
<222>  (144)..(144)
<223>  Xaa is a non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (145)..(145)
<223>  Xaa is an aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (147)..(147)
<223>  Xaa is a non-polar or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (149)..(149)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (150)..(150)
<223>  Xaa is an aromatic or acidic residue

<220>
<221>  MISC_FEATURE
<222>  (153)..(153)
<223>  Xaa is a polar or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (157)..(157)
<223>  Xaa is a non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (158)..(158)
<223>  Xaa is a non-polar or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (173)..(173)
<223>  Xaa is a non-polar or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (175)..(175)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (190)..(190)
<223>  Xaa is a polar, aliphatic, aromatic, or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (195)..(195)
<223>  Xaa is a non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (196)..(196)
<223>  Xaa is a polar, basic, non-polar, aromatic, or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (197)..(197)
<223>  Xaa is an aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (198)..(198)
<223>  Xaa is a polar or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (199)..(199)
<223>  Xaa is an acidic residue

<220>
<221>  MISC_FEATURE
<222>  (201)..(201)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (202)..(202)
<223>  Xaa is an aromatic or basic residue

<220>
<221>  MISC_FEATURE
<222>  (203)..(203)
<223>  Xaa is a non-polar, aromatic, or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (205)..(205)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (206)..(206)
<223>  Xaa is an aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (207)..(207)
<223>  Xaa is a non-polar, aromatic, acidic, or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (209)..(209)
<223>  Xaa is an aromatic, acidic, non-polar, polar, or aliphatic 
       residue

<220>
<221>  MISC_FEATURE
<222>  (210)..(210)
<223>  Xaa is a non-polar or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (211)..(211)
<223>  Xaa is an aromatic, acidic, aliphatic, polar, or non-polar 
       residue

<220>
<221>  MISC_FEATURE
<222>  (212)..(212)
<223>  Xaa is a non-polar, aliphatic, acidic, basic, or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (213)..(213)
<223>  Xaa is an aromatic, basic, acidic, polar, non-polar, or aliphatic
       residue

<220>
<221>  MISC_FEATURE
<222>  (217)..(217)
<223>  Xaa is a polar or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (226)..(226)
<223>  Xaa is an aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (233)..(233)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (250)..(250)
<223>  Xaa is a non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (252)..(252)
<223>  Xaa is an aromatic residue

<400>  245

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Xaa Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Xaa Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Xaa 
    50                  55                  60                  


Ser Asp Glu Xaa Gly Trp Xaa Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Xaa Val Xaa 
                85                  90                  95      


Lys Ser Val Glu Asp Xaa Thr Thr Glu Glu Trp Arg Lys Xaa Leu Ser 
            100                 105                 110         


Val Xaa Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Xaa Ala Ser Ile Ile Asn Met Ser Ser Xaa 
    130                 135                 140                 


Xaa Gly Xaa Val Xaa Xaa Pro Thr Xaa Gly Ala Tyr Xaa Xaa Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Xaa Cys Xaa Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Xaa Ile Lys 
            180                 185                 190         


Thr Pro Xaa Xaa Xaa Xaa Xaa Glu Xaa Xaa Xaa Glu Xaa Xaa Xaa Gln 
        195                 200                 205             


Xaa Xaa Xaa Xaa Xaa Met Gly His Xaa Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Xaa Cys Val Tyr Leu Ala Ser Xaa Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Gly Xaa Ala Xaa 
                245                 250         


<210>  246
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic engineered aldo-keto reductase variant


<220>
<221>  MISC_FEATURE
<222>  (17)..(17)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (40)..(40)
<223>  Xaa is a basic residue

<220>
<221>  MISC_FEATURE
<222>  (64)..(64)
<223>  Xaa is an aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (68)..(68)
<223>  Xaa is a non-polar or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (71)..(71)
<223>  Xaa is a non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (94)..(94)
<223>  Xaa is a polar, aliphatic, or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (96)..(96)
<223>  Xaa is an aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (102)..(102)
<223>  Xaa is an acidic or polar residue

<220>
<221>  MISC_FEATURE
<222>  (110)..(110)
<223>  Xaa is an acidic or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (114)..(114)
<223>  Xaa is a non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (135)..(135)
<223>  Xaa is a basic residue

<220>
<221>  MISC_FEATURE
<222>  (144)..(144)
<223>  Xaa is a non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (145)..(145)
<223>  Xaa is an aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (147)..(147)
<223>  Xaa is a non-polar or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (149)..(149)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (150)..(150)
<223>  Xaa is an aromatic or acidic residue

<220>
<221>  MISC_FEATURE
<222>  (153)..(153)
<223>  Xaa is a polar or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (157)..(157)
<223>  Xaa is a non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (158)..(158)
<223>  Xaa is a non-polar or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (175)..(175)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (190)..(190)
<223>  Xaa is a polar, aliphatic, aromatic, or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (195)..(195)
<223>  Xaa is a non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (196)..(196)
<223>  Xaa is a polar, basic, non-polar, aromatic, or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (197)..(197)
<223>  Xaa is an aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (198)..(198)
<223>  Xaa is a polar or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (199)..(199)
<223>  Xaa is an acidic residue

<220>
<221>  MISC_FEATURE
<222>  (201)..(201)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (202)..(202)
<223>  Xaa is an aromatic or basic residue

<220>
<221>  MISC_FEATURE
<222>  (203)..(203)
<223>  Xaa is a non-polar, aromatic, or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (205)..(205)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (206)..(206)
<223>  Xaa is an aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (207)..(207)
<223>  Xaa is a non-polar, aromatic, acidic, or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (209)..(209)
<223>  Xaa is an aromatic, acidic, non-polar, polar, or aliphatic 
       residue

<220>
<221>  MISC_FEATURE
<222>  (210)..(210)
<223>  Xaa is a non-polar or aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (211)..(211)
<223>  Xaa is an aromatic, acidic, aliphatic, polar, or non-polar 
       residue

<220>
<221>  MISC_FEATURE
<222>  (212)..(212)
<223>  Xaa is a non-polar, aliphatic, acidic, basic, or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (213)..(213)
<223>  Xaa is an aromatic, basic, acidic, polar, non-polar, or aliphatic
       residue

<220>
<221>  MISC_FEATURE
<222>  (217)..(217)
<223>  Xaa is a polar or aromatic residue

<220>
<221>  MISC_FEATURE
<222>  (226)..(226)
<223>  Xaa is an aliphatic residue

<220>
<221>  MISC_FEATURE
<222>  (233)..(233)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (250)..(250)
<223>  Xaa is a non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (252)..(252)
<223>  Xaa is an aromatic residue

<400>  246

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Xaa Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Xaa Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Xaa 
    50                  55                  60                  


Ser Asp Glu Xaa Gly Trp Xaa Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Xaa Val Xaa 
                85                  90                  95      


Lys Ser Val Glu Asp Xaa Thr Thr Glu Glu Trp Arg Lys Xaa Leu Ser 
            100                 105                 110         


Val Xaa Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Xaa Ala Ser Ile Ile Asn Met Ser Ser Xaa 
    130                 135                 140                 


Xaa Gly Xaa Val Xaa Xaa Pro Thr Xaa Gly Ala Tyr Xaa Xaa Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Leu Cys Xaa Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Xaa Ile Lys 
            180                 185                 190         


Thr Pro Xaa Xaa Xaa Xaa Xaa Glu Xaa Xaa Xaa Glu Xaa Xaa Xaa Gln 
        195                 200                 205             


Xaa Xaa Xaa Xaa Xaa Met Gly His Xaa Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Xaa Cys Val Tyr Leu Ala Ser Xaa Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Gly Xaa Ala Xaa 
                245                 250         


