                         SEQUENCE LISTING

<110>  CIBUS US LLC
       CIBUS Europe B.V.
       LINCOLN et al.
 
<120>  MUTATED ACETOHYDROXYACID SYNTHASE GENES IN EUPHORBIACEAE AND 
       PLANT MATERIAL COMPRISING SUCH GENES

<130>  CIBUS-032-PCT

<140>  PCT/US2016/012717
<141>  2016-01-08

<150>  US 62/102,009
<151>  2015-01-10

<150>  US 62/102,010
<151>  2015-01-10

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  658
<212>  PRT
<213>  Manihot esculenta


<220>
<221>  misc_feature
<223>  Amino acid sequence for a wild type Cassava (Manihot esculenta) 
       AHAS protein

<400>  1

Met Ala Ala Ala Ser Thr Ser Ala Ala Thr Thr Ile Pro Lys Pro Ser 
1               5                   10                  15      


Ser His Ile Ser Ser Ser Ser Arg Ser Ser Ile Phe Ile Ser Arg Phe 
            20                  25                  30          


Thr Leu Pro Leu Ser Leu Asn Pro Gln Lys Ala Ile Pro His Arg Ser 
        35                  40                  45              


Leu His Ile Ser Asn Ser Val Ser Lys Pro Thr Thr Pro Ala Pro Ser 
    50                  55                  60                  


Ser Ser Thr Thr Leu Thr Ile Pro Gln Ala Ser Pro Pro Arg Phe Ser 
65                  70                  75                  80  


Pro Asp Glu Ala Arg Lys Gly Ala Asp Ile Leu Val Glu Ala Leu Glu 
                85                  90                  95      


Arg Gln Gly Val Thr Asp Val Phe Ala Tyr Pro Gly Gly Ala Ser Met 
            100                 105                 110         


Glu Ile His Gln Ala Leu Thr Arg Ser Pro Ile Ile Arg Asn Val Leu 
        115                 120                 125             


Pro Arg His Glu Gln Gly Gly Val Phe Ala Ala Glu Gly Tyr Ala Arg 
    130                 135                 140                 


Ala Ser Gly Lys Pro Gly Val Cys Ile Ala Thr Ser Gly Pro Gly Ala 
145                 150                 155                 160 


Thr Asn Leu Val Ser Gly Leu Ala Asp Ala Leu Leu Asp Ser Val Pro 
                165                 170                 175     


Ile Val Ala Ile Thr Gly Gln Val Pro Arg Arg Met Ile Gly Thr Asp 
            180                 185                 190         


Ala Phe Gln Glu Thr Pro Ile Val Glu Val Thr Arg Ser Ile Thr Lys 
        195                 200                 205             


His Asn Tyr Leu Val Leu Asp Val Asp Asp Ile Pro Arg Ile Val Ser 
    210                 215                 220                 


Glu Ala Phe Phe Leu Ala Thr Ser Gly Arg Pro Gly Pro Val Leu Ile 
225                 230                 235                 240 


Asp Val Pro Lys Asp Ile Gln Gln Gln Leu Ala Val Pro Asn Trp Asn 
                245                 250                 255     


Thr Pro Ile Lys Leu Pro Gly Tyr Met Ser Arg Leu Pro Lys Val Pro 
            260                 265                 270         


Asn Glu Ser His Leu Glu Gln Ile Val Arg Leu Ile Phe Glu Ser Lys 
        275                 280                 285             


Lys Pro Val Leu Tyr Val Gly Gly Gly Cys Leu Asn Ser Ser Glu Glu 
    290                 295                 300                 


Leu Arg Lys Phe Val Glu Leu Thr Gly Ile Pro Val Ala Ser Thr Leu 
305                 310                 315                 320 


Met Gly Leu Gly Ala Phe Pro Val Gly His Glu Leu Ser Leu Gln Met 
                325                 330                 335     


Leu Gly Met His Gly Thr Val Tyr Ala Asn Tyr Ser Val Asp Lys Ser 
            340                 345                 350         


Asp Leu Leu Leu Ala Phe Gly Val Arg Phe Asp Asp Arg Val Thr Gly 
        355                 360                 365             


Lys Leu Glu Ala Phe Ala Ser Arg Ala Lys Ile Val His Ile Asp Ile 
    370                 375                 380                 


Asp Ser Ala Glu Ile Gly Lys Asn Lys Gln Pro His Val Ser Val Cys 
385                 390                 395                 400 


Ala Asp Val Lys Phe Ala Leu Gln Gly Met Asn Lys Ile Leu Glu Ser 
                405                 410                 415     


Arg Cys Ala Lys Ser Lys Leu Asp Phe Lys Ala Trp Arg Glu Glu Leu 
            420                 425                 430         


Asn Glu Gln Lys Ser Lys Tyr Pro Leu Lys Tyr Lys Thr Phe Gly Asp 
        435                 440                 445             


Ala Ile Pro Pro Gln Tyr Ala Ile Gln Val Leu Asp Glu Leu Thr Asp 
    450                 455                 460                 


Gly Asn Ala Ile Ile Ser Thr Gly Val Gly Gln His Gln Met Trp Ala 
465                 470                 475                 480 


Ala Gln Phe Tyr Lys Tyr Lys Arg Pro Arg Gln Trp Leu Thr Ser Gly 
                485                 490                 495     


Gly Leu Gly Ala Met Gly Phe Gly Leu Pro Ala Ala Ile Gly Ala Ala 
            500                 505                 510         


Val Ala Asn Pro Gly Ala Val Val Val Asp Ile Asp Gly Asp Gly Ser 
        515                 520                 525             


Phe Ile Met Asn Val Gln Glu Leu Ala Thr Ile Arg Val Glu Asn Leu 
    530                 535                 540                 


Pro Ile Lys Ile Met Leu Leu Asn Asn Gln His Leu Gly Met Val Val 
545                 550                 555                 560 


Gln Trp Glu Asp Arg Phe Tyr Lys Ala Asn Arg Ala His Thr Tyr Leu 
                565                 570                 575     


Gly Asp Pro Ser Lys Glu Ser Glu Ile Phe Pro Asn Met Leu Lys Phe 
            580                 585                 590         


Ala Glu Ala Cys Gly Ile Pro Ala Ala Arg Val Thr Arg Lys Glu Gly 
        595                 600                 605             


Leu Arg Met Ala Ile Gln Lys Met Leu Asp Thr Pro Gly Pro Tyr Leu 
    610                 615                 620                 


Leu Asp Val Ile Val Pro His Gln Glu His Val Leu Pro Met Ile Pro 
625                 630                 635                 640 


Ser Gly Gly Ala Phe Lys Asp Val Ile Thr Glu Gly Asp Gly Arg Thr 
                645                 650                 655     


Lys Tyr 
        


<210>  2
<211>  1977
<212>  DNA
<213>  Manihot esculenta


<220>
<221>  misc_feature
<223>  Nucleotide sequence encoding a wild type Cassava (Manihot 
       esculenta) AHAS protein

<400>  2
atggcggcgg cgtctacctc tgcggctacc actatcccta aaccctcttc tcacatttct       60

tcctcctcca gatcttcaat cttcatttcc agattcaccc tcccattgtc tctcaacccc      120

caaaaggcca ttcctcaccg ctctctccac atatcaaact ctgtctctaa acctacaacc      180

cctgccccct catcctccac caccttaacc attcctcaag cgtctcctcc caggttttct      240

cctgatgaag ctcgaaaagg cgccgacatc ctcgttgaag cgctggaacg ccaaggggtc      300

actgatgtat ttgcttatcc aggcggcgca tccatggaga tccatcaagc cctgactcgc      360

tcacctataa ttcgcaatgt cctcccgcgc catgagcaag gtggggtctt tgcggctgag      420

ggatatgctc gcgcttctgg caagcctggc gtctgtatcg caacctcggg acccggcgct      480

acaaatctcg taagtggctt ggcagacgct ctccttgaca gcgtccccat tgtggctatc      540

accggccaag ttcctcgccg catgattggc accgacgcat tccaagaaac tcccattgtt      600

gaggtaactc ggtcaataac taagcacaat tacctggtcc ttgatgttga tgatattcct      660

agaattgtaa gtgaagcttt ctttttggcc acctcgggac gtcctggccc agttctgatt      720

gatgtaccaa aagatataca acaacaatta gctgttccaa attggaatac acctattaaa      780

ttgcctggtt acatgtcgag gttgcctaaa gtgcccaacg aatcacattt ggagcagatt      840

gtgaggctaa tttttgagtc aaagaaaccg gttttatacg tgggaggtgg gtgtttaaat      900

tcaagtgagg agttgagaaa gtttgtcgag ttaactggga tcccagtggc tagtactttg      960

atggggctcg gagcattccc agttggccac gaattgtcat tacaaatgct tggaatgcat     1020

ggaactgttt atgctaacta ctcggtggat aagagtgatt tgttgcttgc gtttggggtg     1080

aggtttgatg acagggtgac aggcaagctt gaggcctttg caagcagagc taagattgtt     1140

cacattgata ttgattccgc tgagattggg aaaaataagc agccccatgt gtctgtttgt     1200

gcagatgtga agtttgcctt gcaagggatg aacaagattt tggagagcag atgtgctaag     1260

agtaagctag attttaaggc ttggagggag gagttgaatg agcagaaaag taaatatcca     1320

ttgaaataca agacatttgg agatgcaatt cctcctcagt acgccataca agttctcgat     1380

gaattaacag atgggaatgc cattataagt actggcgttg gacaacatca gatgtgggct     1440

gctcaatttt acaagtacaa gagaccacgg caatggttga cgtcaggggg attaggggct     1500

atgggttttg gattgcctgc cgccattggg gctgctgttg ctaatcctgg tgcagttgtt     1560

gtagatattg atggtgatgg aagttttatc atgaatgtcc aggagttggc aacaattcgt     1620

gtggagaatc tgccaattaa aataatgctt ttgaataatc agcatttggg aatggtggta     1680

caatgggagg accgattcta caaggctaat agagctcata cttatttggg ggatccatca     1740

aaggagtctg agattttccc caatatgttg aagtttgcag aagcttgtgg aatacctgct     1800

gctcgcgtga caagaaaaga gggtcttaga atggcgattc agaaaatgct agatactcca     1860

gggccatact tgttggatgt gattgtgccc catcaagaac atgtgctgcc catgatccca     1920

agtgggggag cttttaagga tgtgataact gagggtgatg gaagaacgaa gtattga        1977


<210>  3
<211>  658
<212>  PRT
<213>  Manihot esculenta


<220>
<221>  misc_feature
<223>  Cassava (Manihot esculenta) AHAS comprising a W574S mutation

<400>  3

Met Ala Ala Ala Ser Thr Ser Ala Ala Thr Thr Ile Pro Lys Pro Ser 
1               5                   10                  15      


Ser His Ile Ser Ser Ser Ser Arg Ser Ser Ile Phe Ile Ser Arg Phe 
            20                  25                  30          


Thr Leu Pro Leu Ser Leu Asn Pro Gln Lys Ala Ile Pro His Arg Ser 
        35                  40                  45              


Leu His Ile Ser Asn Ser Val Ser Lys Pro Thr Thr Pro Ala Pro Ser 
    50                  55                  60                  


Ser Ser Thr Thr Leu Thr Ile Pro Gln Ala Ser Pro Pro Arg Phe Ser 
65                  70                  75                  80  


Pro Asp Glu Ala Arg Lys Gly Ala Asp Ile Leu Val Glu Ala Leu Glu 
                85                  90                  95      


Arg Gln Gly Val Thr Asp Val Phe Ala Tyr Pro Gly Gly Ala Ser Met 
            100                 105                 110         


Glu Ile His Gln Ala Leu Thr Arg Ser Pro Ile Ile Arg Asn Val Leu 
        115                 120                 125             


Pro Arg His Glu Gln Gly Gly Val Phe Ala Ala Glu Gly Tyr Ala Arg 
    130                 135                 140                 


Ala Ser Gly Lys Pro Gly Val Cys Ile Ala Thr Ser Gly Pro Gly Ala 
145                 150                 155                 160 


Thr Asn Leu Val Ser Gly Leu Ala Asp Ala Leu Leu Asp Ser Val Pro 
                165                 170                 175     


Ile Val Ala Ile Thr Gly Gln Val Pro Arg Arg Met Ile Gly Thr Asp 
            180                 185                 190         


Ala Phe Gln Glu Thr Pro Ile Val Glu Val Thr Arg Ser Ile Thr Lys 
        195                 200                 205             


His Asn Tyr Leu Val Leu Asp Val Asp Asp Ile Pro Arg Ile Val Ser 
    210                 215                 220                 


Glu Ala Phe Phe Leu Ala Thr Ser Gly Arg Pro Gly Pro Val Leu Ile 
225                 230                 235                 240 


Asp Val Pro Lys Asp Ile Gln Gln Gln Leu Ala Val Pro Asn Trp Asn 
                245                 250                 255     


Thr Pro Ile Lys Leu Pro Gly Tyr Met Ser Arg Leu Pro Lys Val Pro 
            260                 265                 270         


Asn Glu Ser His Leu Glu Gln Ile Val Arg Leu Ile Phe Glu Ser Lys 
        275                 280                 285             


Lys Pro Val Leu Tyr Val Gly Gly Gly Cys Leu Asn Ser Ser Glu Glu 
    290                 295                 300                 


Leu Arg Lys Phe Val Glu Leu Thr Gly Ile Pro Val Ala Ser Thr Leu 
305                 310                 315                 320 


Met Gly Leu Gly Ala Phe Pro Val Gly His Glu Leu Ser Leu Gln Met 
                325                 330                 335     


Leu Gly Met His Gly Thr Val Tyr Ala Asn Tyr Ser Val Asp Lys Ser 
            340                 345                 350         


Asp Leu Leu Leu Ala Phe Gly Val Arg Phe Asp Asp Arg Val Thr Gly 
        355                 360                 365             


Lys Leu Glu Ala Phe Ala Ser Arg Ala Lys Ile Val His Ile Asp Ile 
    370                 375                 380                 


Asp Ser Ala Glu Ile Gly Lys Asn Lys Gln Pro His Val Ser Val Cys 
385                 390                 395                 400 


Ala Asp Val Lys Phe Ala Leu Gln Gly Met Asn Lys Ile Leu Glu Ser 
                405                 410                 415     


Arg Cys Ala Lys Ser Lys Leu Asp Phe Lys Ala Trp Arg Glu Glu Leu 
            420                 425                 430         


Asn Glu Gln Lys Ser Lys Tyr Pro Leu Lys Tyr Lys Thr Phe Gly Asp 
        435                 440                 445             


Ala Ile Pro Pro Gln Tyr Ala Ile Gln Val Leu Asp Glu Leu Thr Asp 
    450                 455                 460                 


Gly Asn Ala Ile Ile Ser Thr Gly Val Gly Gln His Gln Met Trp Ala 
465                 470                 475                 480 


Ala Gln Phe Tyr Lys Tyr Lys Arg Pro Arg Gln Trp Leu Thr Ser Gly 
                485                 490                 495     


Gly Leu Gly Ala Met Gly Phe Gly Leu Pro Ala Ala Ile Gly Ala Ala 
            500                 505                 510         


Val Ala Asn Pro Gly Ala Val Val Val Asp Ile Asp Gly Asp Gly Ser 
        515                 520                 525             


Phe Ile Met Asn Val Gln Glu Leu Ala Thr Ile Arg Val Glu Asn Leu 
    530                 535                 540                 


Pro Ile Lys Ile Met Leu Leu Asn Asn Gln His Leu Gly Met Val Val 
545                 550                 555                 560 


Gln Ser Glu Asp Arg Phe Tyr Lys Ala Asn Arg Ala His Thr Tyr Leu 
                565                 570                 575     


Gly Asp Pro Ser Lys Glu Ser Glu Ile Phe Pro Asn Met Leu Lys Phe 
            580                 585                 590         


Ala Glu Ala Cys Gly Ile Pro Ala Ala Arg Val Thr Arg Lys Glu Gly 
        595                 600                 605             


Leu Arg Met Ala Ile Gln Lys Met Leu Asp Thr Pro Gly Pro Tyr Leu 
    610                 615                 620                 


Leu Asp Val Ile Val Pro His Gln Glu His Val Leu Pro Met Ile Pro 
625                 630                 635                 640 


Ser Gly Gly Ala Phe Lys Asp Val Ile Thr Glu Gly Asp Gly Arg Thr 
                645                 650                 655     


Lys Tyr 
        


<210>  4
<211>  670
<212>  PRT
<213>  Arabidopsis thaliana


<220>
<221>  misc_feature
<223>  Amino acid sequence for Arabidopsis AHAS I

<400>  4

Met Ala Ala Ala Thr Thr Thr Thr Thr Thr Ser Ser Ser Ile Ser Phe 
1               5                   10                  15      


Ser Thr Lys Pro Ser Pro Ser Ser Ser Lys Ser Pro Leu Pro Ile Ser 
            20                  25                  30          


Arg Phe Ser Leu Pro Phe Ser Leu Asn Pro Asn Lys Ser Ser Ser Ser 
        35                  40                  45              


Ser Arg Arg Arg Gly Ile Lys Ser Ser Ser Pro Ser Ser Ile Ser Ala 
    50                  55                  60                  


Val Leu Asn Thr Thr Thr Asn Val Thr Thr Thr Pro Ser Pro Thr Lys 
65                  70                  75                  80  


Pro Thr Lys Pro Glu Thr Phe Ile Ser Arg Phe Ala Pro Asp Gln Pro 
                85                  90                  95      


Arg Lys Gly Ala Asp Ile Leu Val Glu Ala Leu Glu Arg Gln Gly Val 
            100                 105                 110         


Glu Thr Val Phe Ala Tyr Pro Gly Gly Ala Ser Met Glu Ile His Gln 
        115                 120                 125             


Ala Leu Thr Arg Ser Ser Ser Ile Arg Asn Val Leu Pro Arg His Glu 
    130                 135                 140                 


Gln Gly Gly Val Phe Ala Ala Glu Gly Tyr Ala Arg Ser Ser Gly Lys 
145                 150                 155                 160 


Pro Gly Ile Cys Ile Ala Thr Ser Gly Pro Gly Ala Thr Asn Leu Val 
                165                 170                 175     


Ser Gly Leu Ala Asp Ala Leu Leu Asp Ser Val Pro Leu Val Ala Ile 
            180                 185                 190         


Thr Gly Gln Val Pro Arg Arg Met Ile Gly Thr Asp Ala Phe Gln Glu 
        195                 200                 205             


Thr Pro Ile Val Glu Val Thr Arg Ser Ile Thr Lys His Asn Tyr Leu 
    210                 215                 220                 


Val Met Asp Val Glu Asp Ile Pro Arg Ile Ile Glu Glu Ala Phe Phe 
225                 230                 235                 240 


Leu Ala Thr Ser Gly Arg Pro Gly Pro Val Leu Val Asp Val Pro Lys 
                245                 250                 255     


Asp Ile Gln Gln Gln Leu Ala Ile Pro Asn Trp Glu Gln Ala Met Arg 
            260                 265                 270         


Leu Pro Gly Tyr Met Ser Arg Met Pro Lys Pro Pro Glu Asp Ser His 
        275                 280                 285             


Leu Glu Gln Ile Val Arg Leu Ile Ser Glu Ser Lys Lys Pro Val Leu 
    290                 295                 300                 


Tyr Val Gly Gly Gly Cys Leu Asn Ser Ser Asp Glu Leu Gly Arg Phe 
305                 310                 315                 320 


Val Glu Leu Thr Gly Ile Pro Val Ala Ser Thr Leu Met Gly Leu Gly 
                325                 330                 335     


Ser Tyr Pro Cys Asp Asp Glu Leu Ser Leu His Met Leu Gly Met His 
            340                 345                 350         


Gly Thr Val Tyr Ala Asn Tyr Ala Val Glu His Ser Asp Leu Leu Leu 
        355                 360                 365             


Ala Phe Gly Val Arg Phe Asp Asp Arg Val Thr Gly Lys Leu Glu Ala 
    370                 375                 380                 


Phe Ala Ser Arg Ala Lys Ile Val His Ile Asp Ile Asp Ser Ala Glu 
385                 390                 395                 400 


Ile Gly Lys Asn Lys Thr Pro His Val Ser Val Cys Gly Asp Val Lys 
                405                 410                 415     


Leu Ala Leu Gln Gly Met Asn Lys Val Leu Glu Asn Arg Ala Glu Glu 
            420                 425                 430         


Leu Lys Leu Asp Phe Gly Val Trp Arg Asn Glu Leu Asn Val Gln Lys 
        435                 440                 445             


Gln Lys Phe Pro Leu Ser Phe Lys Thr Phe Gly Glu Ala Ile Pro Pro 
    450                 455                 460                 


Gln Tyr Ala Ile Lys Val Leu Asp Glu Leu Thr Asp Gly Lys Ala Ile 
465                 470                 475                 480 


Ile Ser Thr Gly Val Gly Gln His Gln Met Trp Ala Ala Gln Phe Tyr 
                485                 490                 495     


Asn Tyr Lys Lys Pro Arg Gln Trp Leu Ser Ser Gly Gly Leu Gly Ala 
            500                 505                 510         


Met Gly Phe Gly Leu Pro Ala Ala Ile Gly Ala Ser Val Ala Asn Pro 
        515                 520                 525             


Asp Ala Ile Val Val Asp Ile Asp Gly Asp Gly Ser Phe Ile Met Asn 
    530                 535                 540                 


Val Gln Glu Leu Ala Thr Ile Arg Val Glu Asn Leu Pro Val Lys Val 
545                 550                 555                 560 


Leu Leu Leu Asn Asn Gln His Leu Gly Met Val Met Gln Trp Glu Asp 
                565                 570                 575     


Arg Phe Tyr Lys Ala Asn Arg Ala His Thr Phe Leu Gly Asp Pro Ala 
            580                 585                 590         


Gln Glu Asp Glu Ile Phe Pro Asn Met Leu Leu Phe Ala Ala Ala Cys 
        595                 600                 605             


Gly Ile Pro Ala Ala Arg Val Thr Lys Lys Ala Asp Leu Arg Glu Ala 
    610                 615                 620                 


Ile Gln Thr Met Leu Asp Thr Pro Gly Pro Tyr Leu Leu Asp Val Ile 
625                 630                 635                 640 


Cys Pro His Gln Glu His Val Leu Pro Met Ile Pro Ser Gly Gly Thr 
                645                 650                 655     


Phe Asn Asp Val Ile Thr Glu Gly Asp Gly Arg Ile Lys Tyr 
            660                 665                 670 


<210>  5
<211>  658
<212>  PRT
<213>  Manihot esculenta


<220>
<221>  misc_feature
<223>  Amino acid sequence for Cassava (Manihot esculenta) AHAS 
       comprising a D376A mutation

<400>  5

Met Ala Ala Ala Ser Thr Ser Ala Ala Thr Thr Ile Pro Lys Pro Ser 
1               5                   10                  15      


Ser His Ile Ser Ser Ser Ser Arg Ser Ser Ile Phe Ile Ser Arg Phe 
            20                  25                  30          


Thr Leu Pro Leu Ser Leu Asn Pro Gln Lys Ala Ile Pro His Arg Ser 
        35                  40                  45              


Leu His Ile Ser Asn Ser Val Ser Lys Pro Thr Thr Pro Ala Pro Ser 
    50                  55                  60                  


Ser Ser Thr Thr Leu Thr Ile Pro Gln Ala Ser Pro Pro Arg Phe Ser 
65                  70                  75                  80  


Pro Asp Glu Ala Arg Lys Gly Ala Asp Ile Leu Val Glu Ala Leu Glu 
                85                  90                  95      


Arg Gln Gly Val Thr Asp Val Phe Ala Tyr Pro Gly Gly Ala Ser Met 
            100                 105                 110         


Glu Ile His Gln Ala Leu Thr Arg Ser Pro Ile Ile Arg Asn Val Leu 
        115                 120                 125             


Pro Arg His Glu Gln Gly Gly Val Phe Ala Ala Glu Gly Tyr Ala Arg 
    130                 135                 140                 


Ala Ser Gly Lys Pro Gly Val Cys Ile Ala Thr Ser Gly Pro Gly Ala 
145                 150                 155                 160 


Thr Asn Leu Val Ser Gly Leu Ala Asp Ala Leu Leu Asp Ser Val Pro 
                165                 170                 175     


Ile Val Ala Ile Thr Gly Gln Val Pro Arg Arg Met Ile Gly Thr Asp 
            180                 185                 190         


Ala Phe Gln Glu Thr Pro Ile Val Glu Val Thr Arg Ser Ile Thr Lys 
        195                 200                 205             


His Asn Tyr Leu Val Leu Asp Val Asp Asp Ile Pro Arg Ile Val Ser 
    210                 215                 220                 


Glu Ala Phe Phe Leu Ala Thr Ser Gly Arg Pro Gly Pro Val Leu Ile 
225                 230                 235                 240 


Asp Val Pro Lys Asp Ile Gln Gln Gln Leu Ala Val Pro Asn Trp Asn 
                245                 250                 255     


Thr Pro Ile Lys Leu Pro Gly Tyr Met Ser Arg Leu Pro Lys Val Pro 
            260                 265                 270         


Asn Glu Ser His Leu Glu Gln Ile Val Arg Leu Ile Phe Glu Ser Lys 
        275                 280                 285             


Lys Pro Val Leu Tyr Val Gly Gly Gly Cys Leu Asn Ser Ser Glu Glu 
    290                 295                 300                 


Leu Arg Lys Phe Val Glu Leu Thr Gly Ile Pro Val Ala Ser Thr Leu 
305                 310                 315                 320 


Met Gly Leu Gly Ala Phe Pro Val Gly His Glu Leu Ser Leu Gln Met 
                325                 330                 335     


Leu Gly Met His Gly Thr Val Tyr Ala Asn Tyr Ser Val Asp Lys Ser 
            340                 345                 350         


Asp Leu Leu Leu Ala Phe Gly Val Arg Phe Asp Ala Arg Val Thr Gly 
        355                 360                 365             


Lys Leu Glu Ala Phe Ala Ser Arg Ala Lys Ile Val His Ile Asp Ile 
    370                 375                 380                 


Asp Ser Ala Glu Ile Gly Lys Asn Lys Gln Pro His Val Ser Val Cys 
385                 390                 395                 400 


Ala Asp Val Lys Phe Ala Leu Gln Gly Met Asn Lys Ile Leu Glu Ser 
                405                 410                 415     


Arg Cys Ala Lys Ser Lys Leu Asp Phe Lys Ala Trp Arg Glu Glu Leu 
            420                 425                 430         


Asn Glu Gln Lys Ser Lys Tyr Pro Leu Lys Tyr Lys Thr Phe Gly Asp 
        435                 440                 445             


Ala Ile Pro Pro Gln Tyr Ala Ile Gln Val Leu Asp Glu Leu Thr Asp 
    450                 455                 460                 


Gly Asn Ala Ile Ile Ser Thr Gly Val Gly Gln His Gln Met Trp Ala 
465                 470                 475                 480 


Ala Gln Phe Tyr Lys Tyr Lys Arg Pro Arg Gln Trp Leu Thr Ser Gly 
                485                 490                 495     


Gly Leu Gly Ala Met Gly Phe Gly Leu Pro Ala Ala Ile Gly Ala Ala 
            500                 505                 510         


Val Ala Asn Pro Gly Ala Val Val Val Asp Ile Asp Gly Asp Gly Ser 
        515                 520                 525             


Phe Ile Met Asn Val Gln Glu Leu Ala Thr Ile Arg Val Glu Asn Leu 
    530                 535                 540                 


Pro Ile Lys Ile Met Leu Leu Asn Asn Gln His Leu Gly Met Val Val 
545                 550                 555                 560 


Gln Ser Glu Asp Arg Phe Tyr Lys Ala Asn Arg Ala His Thr Tyr Leu 
                565                 570                 575     


Gly Asp Pro Ser Lys Glu Ser Glu Ile Phe Pro Asn Met Leu Lys Phe 
            580                 585                 590         


Ala Glu Ala Cys Gly Ile Pro Ala Ala Arg Val Thr Arg Lys Glu Gly 
        595                 600                 605             


Leu Arg Met Ala Ile Gln Lys Met Leu Asp Thr Pro Gly Pro Tyr Leu 
    610                 615                 620                 


Leu Asp Val Ile Val Pro His Gln Glu His Val Leu Pro Met Ile Pro 
625                 630                 635                 640 


Ser Gly Gly Ala Phe Lys Asp Val Ile Thr Glu Gly Asp Gly Arg Thr 
                645                 650                 655     


Lys Tyr 
        


<210>  6
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: crRNA region spacer sequence

<400>  6
acgaggaggg gcttgggttg tgg                                               23


<210>  7
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: AHAS1 sgRNA

<400>  7
atttgggaat ggtggtacaa tgggagg                                           27


<210>  8
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: AHAS2 sgRNA

<400>  8
gaacaatcaa catctgggta tggttgttca atgg                                   34


<210>  9
<211>  113
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: AHAS1-2-C GRON

<400>  9
tctgccaatt aaaataatgc ttttgaataa tcagcatttg ggaatggtgg tacaatcgga       60

agaccgattc tacaaggcta atagagctca tacttatttg ggggatccat caa             113


<210>  10
<211>  113
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: AHAS2-2-C GRON

<400>  10
acctgcctgt gaagatcttg ttactgaaca atcaacatct gggtatggtt gttcagtcgg       60

aggacagatt ctatcattcc aacagagcac atacatattt agggaaccca tca             113


