                         SEQUENCE LISTING

<110>  Agency for Science, Technology amd Research
 
<120>  Dermatopontin as a therapeutic for metabolic disorders

<130>  9869SG3639

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  201
<212>  PRT
<213>  artificial

<220>
<223>  Recombinant dermatopontin (human)

<400>  1

Met Asp Leu Ser Leu Leu Trp Val Leu Leu Pro Leu Val Thr Met Ala 
1               5                   10                  15      


Trp Gly Gln Tyr Gly Asp Tyr Gly Tyr Pro Tyr Gln Gln Tyr His Asp 
            20                  25                  30          


Tyr Ser Asp Asp Gly Trp Val Asn Leu Asn Arg Gln Gly Phe Ser Tyr 
        35                  40                  45              


Gln Cys Pro Gln Gly Gln Val Ile Val Ala Val Arg Ser Ile Phe Ser 
    50                  55                  60                  


Lys Lys Glu Gly Ser Asp Arg Gln Trp Asn Tyr Ala Cys Met Pro Thr 
65                  70                  75                  80  


Pro Gln Ser Leu Gly Glu Pro Thr Glu Cys Trp Trp Glu Glu Ile Asn 
                85                  90                  95      


Arg Ala Gly Met Glu Trp Tyr Gln Thr Cys Ser Asn Asn Gly Leu Val 
            100                 105                 110         


Ala Gly Phe Gln Ser Arg Tyr Phe Glu Ser Val Leu Asp Arg Glu Trp 
        115                 120                 125             


Gln Phe Tyr Cys Cys Arg Tyr Ser Lys Arg Cys Pro Tyr Ser Cys Trp 
    130                 135                 140                 


Leu Thr Thr Glu Tyr Pro Gly His Tyr Gly Glu Glu Met Asp Met Ile 
145                 150                 155                 160 


Ser Tyr Asn Tyr Asp Tyr Tyr Ile Arg Gly Ala Thr Thr Thr Phe Ser 
                165                 170                 175     


Ala Val Glu Arg Asp Arg Gln Trp Lys Phe Ile Met Cys Arg Met Thr 
            180                 185                 190         


Glu Tyr Asp Cys Glu Phe Ala Asn Val 
        195                 200     


<210>  2
<211>  201
<212>  PRT
<213>  artificial

<220>
<223>  Recombinant dermatopontin (bovine)

<400>  2

Met Asp Leu Thr Leu Leu Trp Val Leu Leu Pro Leu Val Thr Val Ala 
1               5                   10                  15      


Trp Gly Gln Tyr Gly Asp Tyr Gly Tyr Ser Tyr His Gln Tyr His Asp 
            20                  25                  30          


Tyr Ser Asp Asp Gly Trp Val Asn Leu Asn Arg Gln Gly Phe Ser Tyr 
        35                  40                  45              


Gln Cys Pro His Gly Gln Val Val Val Ala Val Arg Ser Ile Phe Asn 
    50                  55                  60                  


Lys Lys Glu Gly Ser Asp Arg Gln Trp Asn Tyr Ala Cys Met Pro Thr 
65                  70                  75                  80  


Pro Gln Ser Leu Gly Glu Pro Thr Glu Cys Trp Trp Glu Glu Ile Asn 
                85                  90                  95      


Arg Ala Gly Met Glu Trp Tyr Gln Thr Cys Ser Asn Asn Gly Leu Val 
            100                 105                 110         


Ala Gly Phe Gln Ser Arg Tyr Phe Glu Ser Val Leu Asp Arg Glu Trp 
        115                 120                 125             


Gln Phe Tyr Cys Cys Arg Tyr Ser Lys Arg Cys Pro Tyr Ser Cys Trp 
    130                 135                 140                 


Leu Thr Thr Glu Tyr Pro Gly His Tyr Gly Glu Glu Met Asp Met Ile 
145                 150                 155                 160 


Ser Tyr Asn Tyr Asp Tyr Tyr Met Arg Gly Ala Thr Thr Thr Phe Ser 
                165                 170                 175     


Ala Val Glu Arg Asp Arg Gln Trp Lys Phe Ile Met Cys Arg Met Thr 
            180                 185                 190         


Asp Tyr Asp Cys Glu Phe Ala Asn Val 
        195                 200     


<210>  3
<211>  201
<212>  PRT
<213>  artificial

<220>
<223>  Recombinant dermatopontin (murine)

<400>  3

Met Asp Leu Thr Leu Leu Trp Val Leu Leu Pro Leu Val Thr Thr Ala 
1               5                   10                  15      


Trp Gly Gln Tyr Gly Gly Tyr Gly Tyr Pro Tyr Gln Gln Tyr Gln Asp 
            20                  25                  30          


Tyr Gly Asp Asp Gly Trp Val Asn Leu Asn Arg Gln Gly Phe Ser Tyr 
        35                  40                  45              


Gln Cys Pro His Gly Gln Val Val Val Ala Val Arg Ser Ile Phe Ser 
    50                  55                  60                  


Lys Lys Glu Gly Ser Asp Arg Gln Trp Asn Tyr Ala Cys Met Pro Thr 
65                  70                  75                  80  


Pro Gln Ser Leu Gly Glu Pro Thr Glu Cys Trp Trp Glu Glu Ile Asn 
                85                  90                  95      


Arg Ala Gly Met Glu Trp Tyr Gln Lys Cys Ser Asn Asn Gly Leu Val 
            100                 105                 110         


Ala Gly Phe Gln Ser Arg Tyr Phe Glu Ser Val Leu Asp Arg Glu Trp 
        115                 120                 125             


Gln Phe Tyr Cys Cys Arg Tyr Ser Lys Arg Cys Pro Tyr Ser Cys Trp 
    130                 135                 140                 


Met Thr Thr Glu Tyr Pro Ser His Tyr Gly Glu Asp Met Asp Met Ile 
145                 150                 155                 160 


Ser Tyr Asp Tyr Asp Phe Tyr Met Arg Gly Ala Thr Thr Thr Phe Ser 
                165                 170                 175     


Ala Val Glu Arg Asp Arg Gln Trp Lys Phe Ile Met Cys Arg Met Thr 
            180                 185                 190         


Asp Tyr Asp Cys Glu Phe Glu Asn Val 
        195                 200     


<210>  4
<211>  183
<212>  PRT
<213>  artificial

<220>
<223>  Recombinant dermatopontin (porcine)

<400>  4

Gln Tyr Gly Asp Tyr Gly Tyr Pro Tyr Gln Gln Tyr His Asp Tyr Ser 
1               5                   10                  15      


Asp Asp Gly Trp Val Asn Leu Asn Arg Gln Gly Phe Ser Tyr Gln Cys 
            20                  25                  30          


Pro His Gly Gln Val Val Val Ala Val Arg Ser Ile Phe Asn Lys Lys 
        35                  40                  45              


Glu Gly Ser Asp Arg Gln Trp Asn Tyr Ala Cys Met Pro Thr Pro Gln 
    50                  55                  60                  


Ser Leu Gly Glu Pro Ser Glu Cys Trp Trp Glu Glu Ile Asn Arg Ala 
65                  70                  75                  80  


Gly Met Glu Trp Tyr Gln Thr Cys Ser Asn Asn Gly Leu Val Ala Gly 
                85                  90                  95      


Phe Gln Ser Arg Tyr Phe Glu Ser Val Leu Asp Arg Glu Trp Gln Phe 
            100                 105                 110         


Tyr Cys Cys Arg Tyr Ser Lys Arg Cys Pro Tyr Ser Cys Trp Met Thr 
        115                 120                 125             


Thr Glu Tyr Pro Gly His Tyr Gly Glu Glu Met Asp Met Ile Ser Tyr 
    130                 135                 140                 


Asn Tyr Asp Tyr Tyr Met Arg Gly Ala Thr Thr Thr Phe Ser Ala Val 
145                 150                 155                 160 


Glu Arg Asp Arg Gln Trp Lys Phe Ile Met Cys Arg Met Thr Asp Tyr 
                165                 170                 175     


Asp Cys Glu Phe Ala Asn Val 
            180             


<210>  5
<211>  606
<212>  DNA
<213>  artificial

<220>
<223>  Human dermatopontin (DPT) nucleic acid sequence used to generate 
       recombinant DPT constructs

<400>  5
atggacctca gtcttctctg ggtacttctg cccctagtca ccatggcctg gggccagtat       60

ggcgattatg gatacccata ccagcagtat catgactaca gcgatgatgg gtgggtgaat      120

ttgaaccggc aaggcttcag ctaccagtgt ccccaggggc aggtgatagt ggccgtgagg      180

agcatcttca gcaagaagga aggttctgac agacaatgga actacgcctg catgcccacg      240

ccacagagcc tcggggaacc cacggagtgc tggtgggagg agatcaacag ggctggcatg      300

gaatggtacc agacgtgctc caacaatggg ctggtggcag gattccagag ccgctacttc      360

gagtcagtgc tggatcggga gtggcagttt tactgttgtc gctacagcaa gaggtgccca      420

tattcctgct ggctaacaat agaatatcca ggtcactatg gtgaggaaat ggacatgatt      480

tcctacaatt atgattacta tatccgagga gcaacaacca ctttctctgc agtggaaagg      540

gatcgccagt ggaagttcat aatgtgccgg atgactgaat acgactgtga atttgcaaat      600

gtttag                                                                 606


<210>  6
<211>  18
<212>  PRT
<213>  artificial

<220>
<223>  Conserved N-terminal secretory sequence (human)

<400>  6

Met Asp Leu Ser Leu Leu Trp Val Leu Leu Pro Leu Val Thr Met Ala 
1               5                   10                  15      


Trp Gly 
        


<210>  7
<211>  18
<212>  PRT
<213>  artificial

<220>
<223>  Conserved N-terminal secretory sequence (bovine)

<400>  7

Met Asp Leu Thr Leu Leu Trp Val Leu Leu Pro Leu Val Thr Val Ala 
1               5                   10                  15      


Trp Gly 
        


<210>  8
<211>  18
<212>  PRT
<213>  artificial

<220>
<223>  Conserved N-terminal secretory sequence (murine)

<400>  8

Met Asp Leu Thr Leu Leu Trp Val Leu Leu Pro Leu Val Thr Thr Ala 
1               5                   10                  15      


Trp Gly 
        


<210>  9
<211>  20
<212>  PRT
<213>  artificial

<220>
<223>  Nuclear localisation sequence of nucleoplasmin

<400>  9

Ala Val Lys Arg Pro Ala Ala Thr Lys Lys Ala Gly Gln Ala Lys Lys 
1               5                   10                  15      


Lys Lys Leu Asp 
            20  


<210>  10
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  Nuclear localisation sequence of c-myc

<400>  10

Pro Ala Ala Lys Arg Val Lys Leu Asp 
1               5                   


<210>  11
<211>  624
<212>  DNA
<213>  artificial

<220>
<223>  His-tagged recombinant dermatopontin (rDPT-His)

<400>  11
atggacctca gtcttctctg ggtacttctg cccctagtca ccatggcctg gggccagtat       60

ggcgattatg gatacccata ccagcagtat catgactaca gcgatgatgg gtgggtgaat      120

ttgaaccggc aaggcttcag ctaccagtgt ccccaggggc aggtgatagt ggccgtgagg      180

agcatcttca gcaagaagga aggttctgac agacaatgga actacgcctg catgcccacg      240

ccacagagcc tcggggaacc cacggagtgc tggtgggagg agatcaacag ggctggcatg      300

gaatggtacc agacgtgctc caacaatggg ctggtggcag gattccagag ccgctacttc      360

gagtcagtgc tggatcggga gtggcagttt tactgttgtc gctacagcaa gaggtgccca      420

tattcctgct ggctaacaat agaatatcca ggtcactatg gtgaggaaat ggacatgatt      480

tcctacaatt atgattacta tatccgagga gcaacaacca ctttctctgc agtggaaagg      540

gatcgccagt ggaagttcat aatgtgccgg atgactgaat acgactgtga atttgcaaat      600

gttcatcatc atcatcatca ttga                                             624


<210>  12
<211>  207
<212>  PRT
<213>  artificial

<220>
<223>  His-tagged recombinant dermatopontin (rDPT-His)

<400>  12

Met Asp Leu Ser Leu Leu Trp Val Leu Leu Pro Leu Val Thr Met Ala 
1               5                   10                  15      


Trp Gly Gln Tyr Gly Asp Tyr Gly Tyr Pro Tyr Gln Gln Tyr His Asp 
            20                  25                  30          


Tyr Ser Asp Asp Gly Trp Val Asn Leu Asn Arg Gln Gly Phe Ser Tyr 
        35                  40                  45              


Gln Cys Pro Gln Gly Gln Val Ile Val Ala Val Arg Ser Ile Phe Ser 
    50                  55                  60                  


Lys Lys Glu Gly Ser Asp Arg Gln Trp Asn Tyr Ala Cys Met Pro Thr 
65                  70                  75                  80  


Pro Gln Ser Leu Gly Glu Pro Thr Glu Cys Trp Trp Glu Glu Ile Asn 
                85                  90                  95      


Arg Ala Gly Met Glu Trp Tyr Gln Thr Cys Ser Asn Asn Gly Leu Val 
            100                 105                 110         


Ala Gly Phe Gln Ser Arg Tyr Phe Glu Ser Val Leu Asp Arg Glu Trp 
        115                 120                 125             


Gln Phe Tyr Cys Cys Arg Tyr Ser Lys Arg Cys Pro Tyr Ser Cys Trp 
    130                 135                 140                 


Leu Thr Thr Glu Tyr Pro Gly His Tyr Gly Glu Glu Met Asp Met Ile 
145                 150                 155                 160 


Ser Tyr Asn Tyr Asp Tyr Tyr Ile Arg Gly Ala Thr Thr Thr Phe Ser 
                165                 170                 175     


Ala Val Glu Arg Asp Arg Gln Trp Lys Phe Ile Met Cys Arg Met Thr 
            180                 185                 190         


Glu Tyr Asp Cys Glu Phe Ala Asn Val His His His His His His 
        195                 200                 205         


