               SEQUENCE LISTING

<110> B.R.A.H.M.S. GmbH

<120> Free Histone Proteins as Biomarkers

<130> X3493 PCT BLN

<150> EP15155599.2
<151> 2015-02-18
<150> US62/114,300
<151> 2015-02-10

<160> 41

<170> BiSSAP 1.3

<210> 1
<211> 102
<212> PRT
<213> Homo sapiens

<220> 
<223> amino acid sequence of human histone H4, uniprot ID P62805.2,
      initial methionine not included

<400> 1
Ser Gly Arg Gly Lys Gly Gly Lys Gly Leu Gly Lys Gly Gly Ala Lys 
1               5                   10                  15      
Arg His Arg Lys Val Leu Arg Asp Asn Ile Gln Gly Ile Thr Lys Pro 
            20                  25                  30          
Ala Ile Arg Arg Leu Ala Arg Arg Gly Gly Val Lys Arg Ile Ser Gly 
        35                  40                  45              
Leu Ile Tyr Glu Glu Thr Arg Gly Val Leu Lys Val Phe Leu Glu Asn 
    50                  55                  60                  
Val Ile Arg Asp Ala Val Thr Tyr Thr Glu His Ala Lys Arg Lys Thr 
65                  70                  75                  80  
Val Thr Ala Met Asp Val Val Tyr Ala Leu Lys Arg Gln Gly Arg Thr 
                85                  90                  95      
Leu Tyr Gly Phe Gly Gly 
            100         

<210> 2
<211> 57
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of 46-102 of SEQ ID NO: 1

<400> 2
Ile Ser Gly Leu Ile Tyr Glu Glu Thr Arg Gly Val Leu Lys Val Phe 
1               5                   10                  15      
Leu Glu Asn Val Ile Arg Asp Ala Val Thr Tyr Thr Glu His Ala Lys 
            20                  25                  30          
Arg Lys Thr Val Thr Ala Met Asp Val Val Tyr Ala Leu Lys Arg Gln 
        35                  40                  45              
Gly Arg Thr Leu Tyr Gly Phe Gly Gly 
    50                  55          

<210> 3
<211> 10
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence 46-55 of SEQ ID NO: 1

<400> 3
Ile Ser Gly Leu Ile Tyr Glu Glu Thr Arg 
1               5                   10  

<210> 4
<211> 8
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence 60-67 of SEQ ID NO: 1

<400> 4
Val Phe Leu Glu Asn Val Ile Arg 
1               5               

<210> 5
<211> 12
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence 80-91 with acetylated K90 of SEQ ID NO: 1

<220> 
<221> MOD_RES
<222> 12
<223> ACETYLATION

<400> 5
Thr Val Thr Ala Met Asp Val Val Tyr Ala Leu Lys 
1               5                   10          

<210> 6
<211> 12
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence 24-35 of SEQ ID NO: 1

<400> 6
Asp Asn Ile Gln Gly Ile Thr Lys Pro Ala Ile Arg 
1               5                   10          

<210> 7
<211> 10
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence 68-77 of SEQ ID NO: 1

<400> 7
Asp Ala Val Thr Tyr Thr Glu His Ala Lys 
1               5                   10  

<210> 8
<211> 16
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of H4-GR-17, position 2-17 of SEQ ID NO:1

<400> 8
Gly Arg Gly Lys Gly Gly Lys Gly Leu Gly Lys Gly Gly Ala Lys Arg 
1               5                   10                  15      



<210> 9
<211> 17
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of H4-GH-18, position 2-18 of SEQ ID NO:1

<400> 9
Gly Arg Gly Lys Gly Gly Lys Gly Leu Gly Lys Gly Gly Ala Lys Arg 
1               5                   10                  15      
His 
    

<210> 10
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of H4-LI-9, position 22-30 of SEQ ID NO:1

<400> 10
Leu Arg Asp Asn Ile Gln Gly Ile Thr 
1               5                   

<210> 11
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of H4-LR-15, position 22-35 of SEQ ID NO:1

<400> 11
Leu Arg Asp Asn Ile Gln Gly Ile Thr Lys Pro Ala Ile Arg 
1               5                   10                  

<210> 12
<211> 16
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of H4-LL-17, position 22-37 of SEQ ID NO:1

<400> 12
Leu Arg Asp Asn Ile Gln Gly Ile Thr Lys Pro Ala Ile Arg Arg Leu 
1               5                   10                  15      



<210> 13
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of H4-PR-15, position 32-45 of SEQ ID NO:1

<400> 13
Pro Ala Ile Arg Arg Leu Ala Arg Arg Gly Gly Val Lys Arg 
1               5                   10                  

<210> 14
<211> 16
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of H4-PS-17, position 32-47 of SEQ ID NO:1

<400> 14
Pro Ala Ile Arg Arg Leu Ala Arg Arg Gly Gly Val Lys Arg Ile Ser 
1               5                   10                  15      



<210> 15
<211> 12
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of H4-EE-13, position 52-63 of SEQ ID NO:1

<400> 15
Glu Glu Thr Arg Gly Val Leu Lys Val Phe Leu Glu 
1               5                   10          

<210> 16
<211> 13
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of H4-EN-14, position 52-64 of SEQ ID NO:1

<400> 16
Glu Glu Thr Arg Gly Val Leu Lys Val Phe Leu Glu Asn 
1               5                   10              

<210> 17
<211> 12
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of H4-RR-13, position 67-78 of SEQ ID NO:1

<400> 17
Arg Asp Ala Val Thr Tyr Thr Glu His Ala Lys Arg 
1               5                   10          

<210> 18
<211> 13
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of H4- RK-14, position 67-79 of SEQ ID NO:1

<400> 18
Arg Asp Ala Val Thr Tyr Thr Glu His Ala Lys Arg Lys 
1               5                   10              

<210> 19
<211> 12
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of H4-TK-13, position 80-91 of SEQ ID NO:1

<400> 19
Thr Val Thr Ala Met Asp Val Val Tyr Ala Leu Lys 
1               5                   10          

<210> 20
<211> 13
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of H4-TR-14, position 80-92 of SEQ ID NO:1

<400> 20
Thr Val Thr Ala Met Asp Val Val Tyr Ala Leu Lys Arg 
1               5                   10              

<210> 21
<211> 8
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of H4-RG-9, position 92-99 of SEQ ID NO:1

<400> 21
Arg Gln Gly Arg Thr Leu Tyr Gly 
1               5               

<210> 22
<211> 11
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of H4-RG-12, position 92-102 of SEQ ID NO:1

<400> 22
Arg Gln Gly Arg Thr Leu Tyr Gly Phe Gly Gly 
1               5                   10      

<210> 23
<211> 130
<212> PRT
<213> Homo sapiens

<220> 
<223> amino acid sequence of human histone H2A type 1, uniprot ID
      Q96QV6, initial methionine not included

<400> 23
Ser Gly Arg Gly Lys Gln Gly Gly Lys Ala Arg Ala Lys Ser Lys Ser 
1               5                   10                  15      
Arg Ser Ser Arg Ala Gly Leu Gln Phe Pro Val Gly Arg Ile His Arg 
            20                  25                  30          
Leu Leu Arg Lys Gly Asn Tyr Ala Glu Arg Ile Gly Ala Gly Ala Pro 
        35                  40                  45              
Val Tyr Leu Ala Ala Val Leu Glu Tyr Leu Thr Ala Glu Ile Leu Glu 
    50                  55                  60                  
Leu Ala Gly Asn Ala Ser Arg Asp Asn Lys Lys Thr Arg Ile Ile Pro 
65                  70                  75                  80  
Arg His Leu Gln Leu Ala Ile Arg Asn Asp Glu Glu Leu Asn Lys Leu 
                85                  90                  95      
Leu Gly Gly Val Thr Ile Ala Gln Gly Gly Val Leu Pro Asn Ile Gln 
            100                 105                 110         
Ala Val Leu Leu Pro Lys Lys Thr Glu Ser His His His Lys Ala Gln 
        115                 120                 125             
Ser Lys 
    130 

<210> 24
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of HA-AR-10, position 21-29 of SEQ ID NO:23

<400> 24
Ala Gly Leu Gln Phe Pro Val Gly Arg 
1               5                   

<210> 25
<211> 11
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of HA-AH-12, position 21-31 of SEQ ID NO:23

<400> 25
Ala Gly Leu Gln Phe Pro Val Gly Arg Ile His 
1               5                   10      

<210> 26
<211> 22
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of HA-IL-23, position 30-51 of SEQ ID NO:23

<400> 26
Ile His Arg Leu Leu Arg Lys Gly Asn Tyr Ala Glu Arg Ile Gly Ala 
1               5                   10                  15      
Gly Ala Pro Val Tyr Leu 
            20          

<210> 27
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of HA-IA-25, position 30-53 of SEQ ID NO:23

<400> 27
Ile His Arg Leu Leu Arg Lys Gly Asn Tyr Ala Glu Arg Ile Gly Ala 
1               5                   10                  15      
Gly Ala Pro Val Tyr Leu Ala Ala 
            20                  

<210> 28
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence 82-88 of SEQ ID NO:23

<400> 28
His Leu Gln Leu Ala Ile Arg 
1               5           

<210> 29
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence 89-95 of SEQ ID NO:23

<400> 29
Asn Asp Glu Glu Leu Asn Lys 
1               5           

<210> 30
<211> 19
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence 100-118 of SEQ ID NO:23

<400> 30
Val Thr Ile Ala Gln Gly Gly Val Leu Pro Asn Ile Gln Ala Val Leu 
1               5                   10                  15      
Leu Pro Lys 
            

<210> 31
<211> 126
<212> PRT
<213> Homo sapiens

<220> 
<223> acid sequence of human histone H2B,uniprot ID P62807

<400> 31
Met Pro Glu Pro Ala Lys Ser Ala Pro Ala Pro Lys Lys Gly Ser Lys 
1               5                   10                  15      
Lys Ala Val Thr Lys Ala Gln Lys Lys Asp Gly Lys Lys Arg Lys Arg 
            20                  25                  30          
Ser Arg Lys Glu Ser Tyr Ser Val Tyr Val Tyr Lys Val Leu Lys Gln 
        35                  40                  45              
Val His Pro Asp Thr Gly Ile Ser Ser Lys Ala Met Gly Ile Met Asn 
    50                  55                  60                  
Ser Phe Val Asn Asp Ile Phe Glu Arg Ile Ala Gly Glu Ala Ser Arg 
65                  70                  75                  80  
Leu Ala His Tyr Asn Lys Arg Ser Thr Ile Thr Ser Arg Glu Ile Gln 
                85                  90                  95      
Thr Ala Val Arg Leu Leu Leu Pro Gly Glu Leu Ala Lys His Ala Val 
            100                 105                 110         
Ser Glu Gly Thr Lys Ala Val Thr Lys Tyr Thr Ser Ser Lys 
        115                 120                 125     

<210> 32
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence 94-100 of SEQ ID NO: 31

<400> 32
Glu Ile Gln Thr Ala Val Arg 
1               5           

<210> 33
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence 101-109 of SEQ ID NO: 31

<400> 33
Leu Leu Leu Pro Gly Glu Leu Ala Lys 
1               5                   

<210> 34
<211> 193
<212> PRT
<213> Homo sapiens

<220> 
<223> amino acid sequence of human histone H2B, uniprot ID Q6DN03.3,
      initial methionine not included

<400> 34
Met Pro Glu Pro Ala Lys Phe Ala Pro Ala Pro Lys Lys Gly Ser Lys 
1               5                   10                  15      
Lys Ala Val Thr Lys Ala Gln Lys Lys Asp Gly Lys Lys Arg Lys Arg 
            20                  25                  30          
Ser Arg Lys Glu Ser Tyr Ser Ile Tyr Val Tyr Lys Val Leu Lys Arg 
        35                  40                  45              
Val His Pro Asp Thr Gly Ile Trp Cys Lys Ala Met Gly Ile Met Asn 
    50                  55                  60                  
Ser Phe Leu Asn Asp Ile Phe Glu Arg Ile Ala Gly Glu Ala Ser Arg 
65                  70                  75                  80  
Leu Ala His Tyr Asn Lys Arg Ser Thr Ile Thr Ser Arg Arg Ser Arg 
                85                  90                  95      
Arg Pro Cys Ala Cys Cys Cys Pro Ala Ser Trp Pro Ser Thr Pro Cys 
            100                 105                 110         
Pro Arg Ala Pro Arg Arg Ser Pro Ser Thr Pro Ala Pro Ser Glu Ser 
        115                 120                 125             
Leu Pro Gly Pro Gly Ala Arg Ser Leu Pro Pro Ser Leu Pro Pro Arg 
    130                 135                 140                 
Val Ala Gly Cys Phe Val Ser Lys Gly Ser Phe Gln Gly His Leu Thr 
145                 150                 155                 160 
Thr Ser Val Lys Glu Ser Phe Leu Cys Cys Gln Ser Gln Leu Met Phe 
                165                 170                 175     
Leu Ala Ser Arg Leu Val Asn Phe Arg Arg Ala His Asn Thr Lys His 
            180                 185                 190         
Arg 
    

<210> 35
<211> 13
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence 181-193 of SEQ ID NO: 34

<400> 35
Leu Val Asn Phe Arg Arg Ala His Asn Thr Lys His Arg 
1               5                   10              

<210> 36
<211> 135
<212> PRT
<213> Homo sapiens

<220> 
<223> amino acid sequence of human histone H3.1 , uniprot ID P68431.2,
      initial methionine not included

<400> 36
Ala Arg Thr Lys Gln Thr Ala Arg Lys Ser Thr Gly Gly Lys Ala Pro 
1               5                   10                  15      
Arg Lys Gln Leu Ala Thr Lys Ala Ala Arg Lys Ser Ala Pro Ala Thr 
            20                  25                  30          
Gly Gly Val Lys Lys Pro His Arg Tyr Arg Pro Gly Thr Val Ala Leu 
        35                  40                  45              
Arg Glu Ile Arg Arg Tyr Gln Lys Ser Thr Glu Leu Leu Ile Arg Lys 
    50                  55                  60                  
Leu Pro Phe Gln Arg Leu Val Arg Glu Ile Ala Gln Asp Phe Lys Thr 
65                  70                  75                  80  
Asp Leu Arg Phe Gln Ser Ser Ala Val Met Ala Leu Gln Glu Ala Cys 
                85                  90                  95      
Glu Ala Tyr Leu Val Gly Leu Phe Glu Asp Thr Asn Leu Cys Ala Ile 
            100                 105                 110         
His Ala Lys Arg Val Thr Ile Met Pro Lys Asp Ile Gln Leu Ala Arg 
        115                 120                 125             
Arg Ile Arg Gly Glu Arg Ala 
    130                 135 

<210> 37
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence 57-63 of SEQ ID NO: 36

<400> 37
Ser Thr Glu Leu Leu Ile Arg 
1               5           

<210> 38
<211> 6
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence 117-122 of SEQ ID NO: 36

<400> 38
Val Thr Ile Met Pro Lys 
1               5       

<210> 39
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence 41-49 of SEQ ID NO: 36

<400> 39
Tyr Arg Pro Gly Thr Val Ala Leu Arg 
1               5                   

<210> 40
<211> 129
<212> PRT
<213> Homo sapiens

<220> 
<223> amino acid sequence of human histone H2A type 3, uniprot ID
      Q7L7L0.3, initial methionine not included), Sigma recombinant H2A
      SRP0406

<400> 40
Ser Gly Arg Gly Lys Gln Gly Gly Lys Ala Arg Ala Lys Ala Lys Ser 
1               5                   10                  15      
Arg Ser Ser Arg Ala Gly Leu Gln Phe Pro Val Gly Arg Val His Arg 
            20                  25                  30          
Leu Leu Arg Lys Gly Asn Tyr Ser Glu Arg Val Gly Ala Gly Ala Pro 
        35                  40                  45              
Val Tyr Leu Ala Ala Val Leu Glu Tyr Leu Thr Ala Glu Ile Leu Glu 
    50                  55                  60                  
Leu Ala Gly Asn Ala Ala Arg Asp Asn Lys Lys Thr Arg Ile Ile Pro 
65                  70                  75                  80  
Arg His Leu Gln Leu Ala Ile Arg Asn Asp Glu Glu Leu Asn Lys Leu 
                85                  90                  95      
Leu Gly Arg Val Thr Ile Ala Gln Gly Gly Val Leu Pro Asn Ile Gln 
            100                 105                 110         
Ala Val Leu Leu Pro Lys Lys Thr Glu Ser His His Lys Ala Lys Gly 
        115                 120                 125             
Lys 
    

<210> 41
<211> 193
<212> PRT
<213> Homo sapiens

<220> 
<223> amino acid sequence of human histone H1, uniprot ID P07305,
      initial methionine not included

<400> 41
Thr Glu Asn Ser Thr Ser Ala Pro Ala Ala Lys Pro Lys Arg Ala Lys 
1               5                   10                  15      
Ala Ser Lys Lys Ser Thr Asp His Pro Lys Tyr Ser Asp Met Ile Val 
            20                  25                  30          
Ala Ala Ile Gln Ala Glu Lys Asn Arg Ala Gly Ser Ser Arg Gln Ser 
        35                  40                  45              
Ile Gln Lys Tyr Ile Lys Ser His Tyr Lys Val Gly Glu Asn Ala Asp 
    50                  55                  60                  
Ser Gln Ile Lys Leu Ser Ile Lys Arg Leu Val Thr Thr Gly Val Leu 
65                  70                  75                  80  
Lys Gln Thr Lys Gly Val Gly Ala Ser Gly Ser Phe Arg Leu Ala Lys 
                85                  90                  95      
Ser Asp Glu Pro Lys Lys Ser Val Ala Phe Lys Lys Thr Lys Lys Glu 
            100                 105                 110         
Ile Lys Lys Val Ala Thr Pro Lys Lys Ala Ser Lys Pro Lys Lys Ala 
        115                 120                 125             
Ala Ser Lys Ala Pro Thr Lys Lys Pro Lys Ala Thr Pro Val Lys Lys 
    130                 135                 140                 
Ala Lys Lys Lys Leu Ala Ala Thr Pro Lys Lys Ala Lys Lys Pro Lys 
145                 150                 155                 160 
Thr Val Lys Ala Lys Pro Val Lys Ala Ser Lys Pro Lys Lys Ala Lys 
                165                 170                 175     
Pro Val Lys Pro Lys Ala Lys Ser Ser Ala Lys Arg Ala Gly Lys Lys 
            180                 185                 190         
Lys 
    

