                         SEQUENCE LISTING

<110>  INSERM
 
<120>  ANTIBODIES SPECIFIC TO GLYCOPROTEIN (GP) OF EBOLA VIRUS AND USES 
       FOR THE TREATMENT AND DIAGNOSIS OF EBOLA INFECTION

<130>  BIO14444 REYNARD-VOLCHKOV

<160>  18    

<170>  PatentIn version 3.3

<210>  1
<211>  363
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleotide sequence of VH domain of mAb 3327

<400>  1
gaggtgcagc ttcaggagtc aggacctagc ctcgggaaac cttctcagac tctgtccctc     60

acctgttctg tcactggcga ctccatcacc agtggttact ggaactggat ccggaaattc    120

ccagggagta agcttgagta catggggtac ataacctcca gtggtagcac ttactacaat    180

ccatctctca aaagtcgcat ctccatcact cgagacacat ccaagaacca gtattacctg    240

cagttgaatt ctgtgacttc tgaggacaca gccacctatt actgtgcaag agaaggggcc    300

cgatcatccg ggggctggta cttcgatgtc tggggcgcag ggaccacggt caccgtctcc    360

tca                                                                  363


<210>  2
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleotide sequence of HCDR1 of mAb 3327

<400>  2
ggcgactcca tcaccagtgg ttactggaac                                      30


<210>  3
<211>  48
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleotide sequence of HCDR2 of mAb 3327

<400>  3
tacataacct ccagtggtag cacttactac aatccatctc tcaaaagt                  48


<210>  4
<211>  39
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleotide sequence of HCDR3 of mAb 3327

<400>  4
gaaggggccc gatcatccgg gggctggtac ttcgatgtc                            39


<210>  5
<211>  121
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of VH domain of mAb 3327

<400>  5

Glu Val Gln Leu Gln Glu Ser Gly Pro Ser Leu Gly Lys Pro Ser Gln 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Ser Val Thr Gly Asp Ser Ile Thr Ser Gly 
            20                  25                  30          


Tyr Trp Asn Trp Ile Arg Lys Phe Pro Gly Ser Lys Leu Glu Tyr Met 
        35                  40                  45              


Gly Tyr Ile Thr Ser Ser Gly Ser Thr Tyr Tyr Asn Pro Ser Leu Lys 
    50                  55                  60                  


Ser Arg Ile Ser Ile Thr Arg Asp Thr Ser Lys Asn Gln Tyr Tyr Leu 
65                  70                  75                  80  


Gln Leu Asn Ser Val Thr Ser Glu Asp Thr Ala Thr Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Glu Gly Ala Arg Ser Ser Gly Gly Trp Tyr Phe Asp Val Trp Gly 
            100                 105                 110         


Ala Gly Thr Thr Val Thr Val Ser Ser 
        115                 120     


<210>  6
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of HCDR1 of mAb 3327

<400>  6

Gly Asp Ser Ile Thr Ser Gly Tyr Trp Asn 
1               5                   10  


<210>  7
<211>  16
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of HCDR2 of mAb 3327

<400>  7

Tyr Ile Thr Ser Ser Gly Ser Thr Tyr Tyr Asn Pro Ser Leu Lys Ser 
1               5                   10                  15      


<210>  8
<211>  13
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of HCDR3  of mAb 3327

<400>  8

Glu Gly Ala Arg Ser Ser Gly Gly Trp Tyr Phe Asp Val 
1               5                   10              


<210>  9
<211>  324
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleotide sequence of VL domain of mAb 3327

<400>  9
gacattgtga tgacccagtc tcacaaattc atgtccacat cagtaggaga cagggtcagc     60

atcacctgca aggccagtca ggatgtgagt tctgctgtag tttggtatca acaaaaacca    120

gggcaatctc ctaaactact gatttactgg gcatccaccc ggcacactgg agtccctgat    180

cgcttcacag gcagtggatc tgggacagat tatactctca ccatcagcag tgtgcaggct    240

gaagacctgg cactttatta ctgtcagcaa cattatagca ctccgacgtt cggtggaggc    300

accaagctgg aaatcaaacg ggct                                           324


<210>  10
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleotide sequence of LCDR1 of mAb 3327

<400>  10
aaggccagtc aggatgtgag ttctgctgta gtt                                  33


<210>  11
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleotide sequence of LCDR2 of mAb 3327

<400>  11
tgggcatcca cccggcacac t                                               21


<210>  12
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleotide sequence of LCDR3 of mAb 3327

<400>  12
cagcaacatt atagcactcc gacg                                            24


<210>  13
<211>  108
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of VL domain of mAb 3327

<400>  13

Asp Ile Val Met Thr Gln Ser His Lys Phe Met Ser Thr Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Ser Ile Thr Cys Lys Ala Ser Gln Asp Val Ser Ser Ala 
            20                  25                  30          


Val Val Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Trp Ala Ser Thr Arg His Thr Gly Val Pro Asp Arg Phe Thr Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Thr Leu Thr Ile Ser Ser Val Gln Ala 
65                  70                  75                  80  


Glu Asp Leu Ala Leu Tyr Tyr Cys Gln Gln His Tyr Ser Thr Pro Thr 
                85                  90                  95      


Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ala 
            100                 105             


<210>  14
<211>  11
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of LCDR1 of mAb 3327

<400>  14

Lys Ala Ser Gln Asp Val Ser Ser Ala Val Val 
1               5                   10      


<210>  15
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of LCDR2 of mAb 3327

<400>  15

Trp Ala Ser Thr Arg His Thr 
1               5           


<210>  16
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of LCDR3 of mAb 3327

<400>  16

Gln Gln His Tyr Ser Thr Pro Thr 
1               5               


<210>  17
<211>  32
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of peptide fused to MBP

<400>  17

Gly Gly Ser His His His His His His Gly Gly Ser Gln Asp Glu Gly 
1               5                   10                  15      


Ala Ala Ile Gly Leu Ala Trp Ile Pro Tyr Phe Gly Pro Ala Ala Glu 
            20                  25                  30          


<210>  18
<211>  676
<212>  PRT
<213>  Ebola virus

<400>  18

Met Gly Val Thr Gly Ile Leu Gln Leu Pro Arg Asp Arg Phe Lys Arg 
1               5                   10                  15      


Thr Ser Phe Phe Leu Trp Val Ile Ile Leu Phe Gln Arg Thr Phe Ser 
            20                  25                  30          


Ile Pro Leu Gly Val Ile His Asn Ser Thr Leu Gln Val Ser Asp Val 
        35                  40                  45              


Asp Lys Leu Val Cys Arg Asp Lys Leu Ser Ser Thr Asn Gln Leu Arg 
    50                  55                  60                  


Ser Val Gly Leu Asn Leu Glu Gly Asn Gly Val Ala Thr Asp Val Pro 
65                  70                  75                  80  


Ser Ala Thr Lys Arg Trp Gly Phe Arg Ser Gly Val Pro Pro Lys Val 
                85                  90                  95      


Val Asn Tyr Glu Ala Gly Glu Trp Ala Glu Asn Cys Tyr Asn Leu Glu 
            100                 105                 110         


Ile Lys Lys Pro Asp Gly Ser Glu Cys Leu Pro Ala Ala Pro Asp Gly 
        115                 120                 125             


Ile Arg Gly Phe Pro Arg Cys Arg Tyr Val His Lys Val Ser Gly Thr 
    130                 135                 140                 


Gly Pro Cys Ala Gly Asp Phe Ala Phe His Lys Glu Gly Ala Phe Phe 
145                 150                 155                 160 


Leu Tyr Asp Arg Leu Ala Ser Thr Val Ile Tyr Arg Gly Thr Thr Phe 
                165                 170                 175     


Ala Glu Gly Val Val Ala Phe Leu Ile Leu Pro Gln Ala Lys Lys Asp 
            180                 185                 190         


Phe Phe Ser Ser His Pro Leu Arg Glu Pro Val Asn Ala Thr Glu Asp 
        195                 200                 205             


Pro Ser Ser Gly Tyr Tyr Ser Thr Thr Ile Arg Tyr Gln Ala Thr Gly 
    210                 215                 220                 


Phe Gly Thr Asn Glu Thr Glu Tyr Leu Phe Glu Val Asp Asn Leu Thr 
225                 230                 235                 240 


Tyr Val Gln Leu Glu Ser Arg Phe Thr Pro Gln Phe Leu Leu Gln Leu 
                245                 250                 255     


Asn Glu Thr Ile Tyr Thr Ser Gly Lys Arg Ser Asn Thr Thr Gly Lys 
            260                 265                 270         


Leu Ile Trp Lys Val Asn Pro Glu Ile Asp Thr Thr Ile Gly Glu Trp 
        275                 280                 285             


Ala Phe Trp Glu Thr Lys Lys Asn Leu Thr Arg Lys Ile Arg Ser Glu 
    290                 295                 300                 


Glu Leu Ser Phe Thr Val Val Ser Asn Gly Ala Lys Asn Ile Ser Gly 
305                 310                 315                 320 


Gln Ser Pro Ala Arg Thr Ser Ser Asp Pro Gly Thr Asn Thr Thr Thr 
                325                 330                 335     


Glu Asp His Lys Ile Met Ala Ser Glu Asn Ser Ser Ala Met Val Gln 
            340                 345                 350         


Val His Ser Gln Gly Arg Glu Ala Ala Val Ser His Leu Thr Thr Leu 
        355                 360                 365             


Ala Thr Ile Ser Thr Ser Pro Gln Ser Leu Thr Thr Lys Pro Gly Pro 
    370                 375                 380                 


Asp Asn Ser Thr His Asn Thr Pro Val Tyr Lys Leu Asp Ile Ser Glu 
385                 390                 395                 400 


Ala Thr Gln Val Glu Gln His His Arg Arg Thr Asp Asn Asp Ser Thr 
                405                 410                 415     


Ala Ser Asp Thr Pro Ser Ala Thr Thr Ala Ala Gly Pro Pro Lys Ala 
            420                 425                 430         


Glu Asn Thr Asn Thr Ser Lys Ser Thr Asp Phe Leu Asp Pro Ala Thr 
        435                 440                 445             


Thr Thr Ser Pro Gln Asn His Ser Glu Thr Ala Gly Asn Asn Asn Thr 
    450                 455                 460                 


His His Gln Asp Thr Gly Glu Glu Ser Ala Ser Ser Gly Lys Leu Gly 
465                 470                 475                 480 


Leu Ile Thr Asn Thr Ile Ala Gly Val Ala Gly Leu Ile Thr Gly Gly 
                485                 490                 495     


Arg Arg Thr Arg Arg Glu Ala Ile Val Asn Ala Gln Pro Lys Cys Asn 
            500                 505                 510         


Pro Asn Leu His Tyr Trp Thr Thr Gln Asp Glu Gly Ala Ala Ile Gly 
        515                 520                 525             


Leu Ala Trp Ile Pro Tyr Phe Gly Pro Ala Ala Glu Gly Ile Tyr Ile 
    530                 535                 540                 


Glu Gly Leu Met His Asn Gln Asp Gly Leu Ile Cys Gly Leu Arg Gln 
545                 550                 555                 560 


Leu Ala Asn Glu Thr Thr Gln Ala Leu Gln Leu Phe Leu Arg Ala Thr 
                565                 570                 575     


Thr Glu Leu Arg Thr Phe Ser Ile Leu Asn Arg Lys Ala Ile Asp Phe 
            580                 585                 590         


Leu Leu Gln Arg Trp Gly Gly Thr Cys His Ile Leu Gly Pro Asp Cys 
        595                 600                 605             


Cys Ile Glu Pro His Asp Trp Thr Lys Asn Ile Thr Asp Lys Ile Asp 
    610                 615                 620                 


Gln Ile Ile His Asp Phe Val Asp Lys Thr Leu Pro Asp Gln Gly Asp 
625                 630                 635                 640 


Asn Asp Asn Trp Trp Thr Gly Trp Arg Gln Trp Ile Pro Ala Gly Ile 
                645                 650                 655     


Gly Val Thr Gly Val Ile Ile Ala Val Ile Ala Leu Phe Cys Ile Cys 
            660                 665                 670         


Lys Phe Val Phe 
        675     


