                         SEQUENCE LISTING

<110>  THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA
       PETER MACCALLUM CANCER CENTRE
 
<120>  LISTERIA-BASED IMMUNOMODULATION

<130>  P-78794-PC

<150>  62/111,156
<151>  2015-02-03

<150>  PCT/US15/66885
<151>  2015-12-18

<160>  51    

<170>  PatentIn version 3.5

<210>  1
<211>  1329
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  recombinant nucleotide encoding the LLO protein

<400>  1
atgaaaaaaa taatgctagt ttttattaca cttatattag ttagtctacc aattgcgcaa       60

caaactgaag caaaggatgc atctgcattc aataaagaaa attcaatttc atccatggca      120

ccaccagcat ctccgcctgc aagtcctaag acgccaatcg aaaagaaaca cgcggatgaa      180

atcgataagt atatacaagg attggattac aataaaaaca atgtattagt ataccacgga      240

gatgcagtga caaatgtgcc gccaagaaaa ggttacaaag atggaaatga atatattgtt      300

gtggagaaaa agaagaaatc catcaatcaa aataatgcag acattcaagt tgtgaatgca      360

atttcgagcc taacctatcc aggtgctctc gtaaaagcga attcggaatt agtagaaaat      420

caaccagatg ttctccctgt aaaacgtgat tcattaacac tcagcattga tttgccaggt      480

atgactaatc aagacaataa aatagttgta aaaaatgcca ctaaatcaaa cgttaacaac      540

gcagtaaata cattagtgga aagatggaat gaaaaatatg ctcaagctta tccaaatgta      600

agtgcaaaaa ttgattatga tgacgaaatg gcttacagtg aatcacaatt aattgcgaaa      660

tttggtacag catttaaagc tgtaaataat agcttgaatg taaacttcgg cgcaatcagt      720

gaagggaaaa tgcaagaaga agtcattagt tttaaacaaa tttactataa cgtgaatgtt      780

aatgaaccta caagaccttc cagatttttc ggcaaagctg ttactaaaga gcagttgcaa      840

gcgcttggag tgaatgcaga aaatcctcct gcatatatct caagtgtggc gtatggccgt      900

caagtttatt tgaaattatc aactaattcc catagtacta aagtaaaagc tgcttttgat      960

gctgccgtaa gcggaaaatc tgtctcaggt gatgtagaac taacaaatat catcaaaaat     1020

tcttccttca aagccgtaat ttacggaggt tccgcaaaag atgaagttca aatcatcgac     1080

ggcaacctcg gagacttacg cgatattttg aaaaaaggcg ctacttttaa tcgagaaaca     1140

ccaggagttc ccattgctta tacaacaaac ttcctaaaag acaatgaatt agctgttatt     1200

aaaaacaact cagaatatat tgaaacaact tcaaaagctt atacagatgg aaaaattaac     1260

atcgatcact ctggaggata cgttgctcaa ttcaacattt cttgggatga agtaaattat     1320

gatctcgag                                                             1329


<210>  2
<211>  442
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LLO protein

<400>  2

Met Lys Lys Ile Met Leu Val Phe Ile Thr Leu Ile Leu Val Ser Leu 
1               5                   10                  15      


Pro Ile Ala Gln Gln Thr Glu Ala Lys Asp Ala Ser Ala Phe Asn Lys 
            20                  25                  30          


Glu Asn Ser Ile Ser Ser Met Ala Pro Pro Ala Ser Pro Pro Ala Ser 
        35                  40                  45              


Pro Lys Thr Pro Ile Glu Lys Lys His Ala Asp Glu Ile Asp Lys Tyr 
    50                  55                  60                  


Ile Gln Gly Leu Asp Tyr Asn Lys Asn Asn Val Leu Val Tyr His Gly 
65                  70                  75                  80  


Asp Ala Val Thr Asn Val Pro Pro Arg Lys Gly Tyr Lys Asp Gly Asn 
                85                  90                  95      


Glu Tyr Ile Val Val Glu Lys Lys Lys Lys Ser Ile Asn Gln Asn Asn 
            100                 105                 110         


Ala Asp Ile Gln Val Val Asn Ala Ile Ser Ser Leu Thr Tyr Pro Gly 
        115                 120                 125             


Ala Leu Val Lys Ala Asn Ser Glu Leu Val Glu Asn Gln Pro Asp Val 
    130                 135                 140                 


Leu Pro Val Lys Arg Asp Ser Leu Thr Leu Ser Ile Asp Leu Pro Gly 
145                 150                 155                 160 


Met Thr Asn Gln Asp Asn Lys Ile Val Val Lys Asn Ala Thr Lys Ser 
                165                 170                 175     


Asn Val Asn Asn Ala Val Asn Thr Leu Val Glu Arg Trp Asn Glu Lys 
            180                 185                 190         


Tyr Ala Gln Ala Tyr Pro Asn Val Ser Ala Lys Ile Asp Tyr Asp Asp 
        195                 200                 205             


Glu Met Ala Tyr Ser Glu Ser Gln Leu Ile Ala Lys Phe Gly Thr Ala 
    210                 215                 220                 


Phe Lys Ala Val Asn Asn Ser Leu Asn Val Asn Phe Gly Ala Ile Ser 
225                 230                 235                 240 


Glu Gly Lys Met Gln Glu Glu Val Ile Ser Phe Lys Gln Ile Tyr Tyr 
                245                 250                 255     


Asn Val Asn Val Asn Glu Pro Thr Arg Pro Ser Arg Phe Phe Gly Lys 
            260                 265                 270         


Ala Val Thr Lys Glu Gln Leu Gln Ala Leu Gly Val Asn Ala Glu Asn 
        275                 280                 285             


Pro Pro Ala Tyr Ile Ser Ser Val Ala Tyr Gly Arg Gln Val Tyr Leu 
    290                 295                 300                 


Lys Leu Ser Thr Asn Ser His Ser Thr Lys Val Lys Ala Ala Phe Asp 
305                 310                 315                 320 


Ala Ala Val Ser Gly Lys Ser Val Ser Gly Asp Val Glu Leu Thr Asn 
                325                 330                 335     


Ile Ile Lys Asn Ser Ser Phe Lys Ala Val Ile Tyr Gly Gly Ser Ala 
            340                 345                 350         


Lys Asp Glu Val Gln Ile Ile Asp Gly Asn Leu Gly Asp Leu Arg Asp 
        355                 360                 365             


Ile Leu Lys Lys Gly Ala Thr Phe Asn Arg Glu Thr Pro Gly Val Pro 
    370                 375                 380                 


Ile Ala Tyr Thr Thr Asn Phe Leu Lys Asp Asn Glu Leu Ala Val Ile 
385                 390                 395                 400 


Lys Asn Asn Ser Glu Tyr Ile Glu Thr Thr Ser Lys Ala Tyr Thr Asp 
                405                 410                 415     


Gly Lys Ile Asn Ile Asp His Ser Gly Gly Tyr Val Ala Gln Phe Asn 
            420                 425                 430         


Ile Ser Trp Asp Glu Val Asn Tyr Asp Leu 
        435                 440         


<210>  3
<211>  529
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LLO protein

<400>  3

Met Lys Lys Ile Met Leu Val Phe Ile Thr Leu Ile Leu Val Ser Leu 
1               5                   10                  15      


Pro Ile Ala Gln Gln Thr Glu Ala Lys Asp Ala Ser Ala Phe Asn Lys 
            20                  25                  30          


Glu Asn Ser Ile Ser Ser Met Ala Pro Pro Ala Ser Pro Pro Ala Ser 
        35                  40                  45              


Pro Lys Thr Pro Ile Glu Lys Lys His Ala Asp Glu Ile Asp Lys Tyr 
    50                  55                  60                  


Ile Gln Gly Leu Asp Tyr Asn Lys Asn Asn Val Leu Val Tyr His Gly 
65                  70                  75                  80  


Asp Ala Val Thr Asn Val Pro Pro Arg Lys Gly Tyr Lys Asp Gly Asn 
                85                  90                  95      


Glu Tyr Ile Val Val Glu Lys Lys Lys Lys Ser Ile Asn Gln Asn Asn 
            100                 105                 110         


Ala Asp Ile Gln Val Val Asn Ala Ile Ser Ser Leu Thr Tyr Pro Gly 
        115                 120                 125             


Ala Leu Val Lys Ala Asn Ser Glu Leu Val Glu Asn Gln Pro Asp Val 
    130                 135                 140                 


Leu Pro Val Lys Arg Asp Ser Leu Thr Leu Ser Ile Asp Leu Pro Gly 
145                 150                 155                 160 


Met Thr Asn Gln Asp Asn Lys Ile Val Val Lys Asn Ala Thr Lys Ser 
                165                 170                 175     


Asn Val Asn Asn Ala Val Asn Thr Leu Val Glu Arg Trp Asn Glu Lys 
            180                 185                 190         


Tyr Ala Gln Ala Tyr Pro Asn Val Ser Ala Lys Ile Asp Tyr Asp Asp 
        195                 200                 205             


Glu Met Ala Tyr Ser Glu Ser Gln Leu Ile Ala Lys Phe Gly Thr Ala 
    210                 215                 220                 


Phe Lys Ala Val Asn Asn Ser Leu Asn Val Asn Phe Gly Ala Ile Ser 
225                 230                 235                 240 


Glu Gly Lys Met Gln Glu Glu Val Ile Ser Phe Lys Gln Ile Tyr Tyr 
                245                 250                 255     


Asn Val Asn Val Asn Glu Pro Thr Arg Pro Ser Arg Phe Phe Gly Lys 
            260                 265                 270         


Ala Val Thr Lys Glu Gln Leu Gln Ala Leu Gly Val Asn Ala Glu Asn 
        275                 280                 285             


Pro Pro Ala Tyr Ile Ser Ser Val Ala Tyr Gly Arg Gln Val Tyr Leu 
    290                 295                 300                 


Lys Leu Ser Thr Asn Ser His Ser Thr Lys Val Lys Ala Ala Phe Asp 
305                 310                 315                 320 


Ala Ala Val Ser Gly Lys Ser Val Ser Gly Asp Val Glu Leu Thr Asn 
                325                 330                 335     


Ile Ile Lys Asn Ser Ser Phe Lys Ala Val Ile Tyr Gly Gly Ser Ala 
            340                 345                 350         


Lys Asp Glu Val Gln Ile Ile Asp Gly Asn Leu Gly Asp Leu Arg Asp 
        355                 360                 365             


Ile Leu Lys Lys Gly Ala Thr Phe Asn Arg Glu Thr Pro Gly Val Pro 
    370                 375                 380                 


Ile Ala Tyr Thr Thr Asn Phe Leu Lys Asp Asn Glu Leu Ala Val Ile 
385                 390                 395                 400 


Lys Asn Asn Ser Glu Tyr Ile Glu Thr Thr Ser Lys Ala Tyr Thr Asp 
                405                 410                 415     


Gly Lys Ile Asn Ile Asp His Ser Gly Gly Tyr Val Ala Gln Phe Asn 
            420                 425                 430         


Ile Ser Trp Asp Glu Val Asn Tyr Asp Pro Glu Gly Asn Glu Ile Val 
        435                 440                 445             


Gln His Lys Asn Trp Ser Glu Asn Asn Lys Ser Lys Leu Ala His Phe 
    450                 455                 460                 


Thr Ser Ser Ile Tyr Leu Pro Gly Asn Ala Arg Asn Ile Asn Val Tyr 
465                 470                 475                 480 


Ala Lys Glu Cys Thr Gly Leu Ala Trp Glu Trp Trp Arg Thr Val Ile 
                485                 490                 495     


Asp Asp Arg Asn Leu Pro Leu Val Lys Asn Arg Asn Ile Ser Ile Trp 
            500                 505                 510         


Gly Thr Thr Leu Tyr Pro Lys Tyr Ser Asn Lys Val Asp Asn Pro Ile 
        515                 520                 525             


Glu 
    


<210>  4
<211>  441
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  the N-terminal fragment of an LLO protein

<400>  4

Met Lys Lys Ile Met Leu Val Phe Ile Thr Leu Ile Leu Val Ser Leu 
1               5                   10                  15      


Pro Ile Ala Gln Gln Thr Glu Ala Lys Asp Ala Ser Ala Phe Asn Lys 
            20                  25                  30          


Glu Asn Ser Ile Ser Ser Val Ala Pro Pro Ala Ser Pro Pro Ala Ser 
        35                  40                  45              


Pro Lys Thr Pro Ile Glu Lys Lys His Ala Asp Glu Ile Asp Lys Tyr 
    50                  55                  60                  


Ile Gln Gly Leu Asp Tyr Asn Lys Asn Asn Val Leu Val Tyr His Gly 
65                  70                  75                  80  


Asp Ala Val Thr Asn Val Pro Pro Arg Lys Gly Tyr Lys Asp Gly Asn 
                85                  90                  95      


Glu Tyr Ile Val Val Glu Lys Lys Lys Lys Ser Ile Asn Gln Asn Asn 
            100                 105                 110         


Ala Asp Ile Gln Val Val Asn Ala Ile Ser Ser Leu Thr Tyr Pro Gly 
        115                 120                 125             


Ala Leu Val Lys Ala Asn Ser Glu Leu Val Glu Asn Gln Pro Asp Val 
    130                 135                 140                 


Leu Pro Val Lys Arg Asp Ser Leu Thr Leu Ser Ile Asp Leu Pro Gly 
145                 150                 155                 160 


Met Thr Asn Gln Asp Asn Lys Ile Val Val Lys Asn Ala Thr Lys Ser 
                165                 170                 175     


Asn Val Asn Asn Ala Val Asn Thr Leu Val Glu Arg Trp Asn Glu Lys 
            180                 185                 190         


Tyr Ala Gln Ala Tyr Ser Asn Val Ser Ala Lys Ile Asp Tyr Asp Asp 
        195                 200                 205             


Glu Met Ala Tyr Ser Glu Ser Gln Leu Ile Ala Lys Phe Gly Thr Ala 
    210                 215                 220                 


Phe Lys Ala Val Asn Asn Ser Leu Asn Val Asn Phe Gly Ala Ile Ser 
225                 230                 235                 240 


Glu Gly Lys Met Gln Glu Glu Val Ile Ser Phe Lys Gln Ile Tyr Tyr 
                245                 250                 255     


Asn Val Asn Val Asn Glu Pro Thr Arg Pro Ser Arg Phe Phe Gly Lys 
            260                 265                 270         


Ala Val Thr Lys Glu Gln Leu Gln Ala Leu Gly Val Asn Ala Glu Asn 
        275                 280                 285             


Pro Pro Ala Tyr Ile Ser Ser Val Ala Tyr Gly Arg Gln Val Tyr Leu 
    290                 295                 300                 


Lys Leu Ser Thr Asn Ser His Ser Thr Lys Val Lys Ala Ala Phe Asp 
305                 310                 315                 320 


Ala Ala Val Ser Gly Lys Ser Val Ser Gly Asp Val Glu Leu Thr Asn 
                325                 330                 335     


Ile Ile Lys Asn Ser Ser Phe Lys Ala Val Ile Tyr Gly Gly Ser Ala 
            340                 345                 350         


Lys Asp Glu Val Gln Ile Ile Asp Gly Asn Leu Gly Asp Leu Arg Asp 
        355                 360                 365             


Ile Leu Lys Lys Gly Ala Thr Phe Asn Arg Glu Thr Pro Gly Val Pro 
    370                 375                 380                 


Ile Ala Tyr Thr Thr Asn Phe Leu Lys Asp Asn Glu Leu Ala Val Ile 
385                 390                 395                 400 


Lys Asn Asn Ser Glu Tyr Ile Glu Thr Thr Ser Lys Ala Tyr Thr Asp 
                405                 410                 415     


Gly Lys Ile Asn Ile Asp His Ser Gly Gly Tyr Val Ala Gln Phe Asn 
            420                 425                 430         


Ile Ser Trp Asp Glu Val Asn Tyr Asp 
        435                 440     


<210>  5
<211>  416
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LLO fragment

<400>  5

Met Lys Lys Ile Met Leu Val Phe Ile Thr Leu Ile Leu Val Ser Leu 
1               5                   10                  15      


Pro Ile Ala Gln Gln Thr Glu Ala Lys Asp Ala Ser Ala Phe Asn Lys 
            20                  25                  30          


Glu Asn Ser Ile Ser Ser Val Ala Pro Pro Ala Ser Pro Pro Ala Ser 
        35                  40                  45              


Pro Lys Thr Pro Ile Glu Lys Lys His Ala Asp Glu Ile Asp Lys Tyr 
    50                  55                  60                  


Ile Gln Gly Leu Asp Tyr Asn Lys Asn Asn Val Leu Val Tyr His Gly 
65                  70                  75                  80  


Asp Ala Val Thr Asn Val Pro Pro Arg Lys Gly Tyr Lys Asp Gly Asn 
                85                  90                  95      


Glu Tyr Ile Val Val Glu Lys Lys Lys Lys Ser Ile Asn Gln Asn Asn 
            100                 105                 110         


Ala Asp Ile Gln Val Val Asn Ala Ile Ser Ser Leu Thr Tyr Pro Gly 
        115                 120                 125             


Ala Leu Val Lys Ala Asn Ser Glu Leu Val Glu Asn Gln Pro Asp Val 
    130                 135                 140                 


Leu Pro Val Lys Arg Asp Ser Leu Thr Leu Ser Ile Asp Leu Pro Gly 
145                 150                 155                 160 


Met Thr Asn Gln Asp Asn Lys Ile Val Val Lys Asn Ala Thr Lys Ser 
                165                 170                 175     


Asn Val Asn Asn Ala Val Asn Thr Leu Val Glu Arg Trp Asn Glu Lys 
            180                 185                 190         


Tyr Ala Gln Ala Tyr Ser Asn Val Ser Ala Lys Ile Asp Tyr Asp Asp 
        195                 200                 205             


Glu Met Ala Tyr Ser Glu Ser Gln Leu Ile Ala Lys Phe Gly Thr Ala 
    210                 215                 220                 


Phe Lys Ala Val Asn Asn Ser Leu Asn Val Asn Phe Gly Ala Ile Ser 
225                 230                 235                 240 


Glu Gly Lys Met Gln Glu Glu Val Ile Ser Phe Lys Gln Ile Tyr Tyr 
                245                 250                 255     


Asn Val Asn Val Asn Glu Pro Thr Arg Pro Ser Arg Phe Phe Gly Lys 
            260                 265                 270         


Ala Val Thr Lys Glu Gln Leu Gln Ala Leu Gly Val Asn Ala Glu Asn 
        275                 280                 285             


Pro Pro Ala Tyr Ile Ser Ser Val Ala Tyr Gly Arg Gln Val Tyr Leu 
    290                 295                 300                 


Lys Leu Ser Thr Asn Ser His Ser Thr Lys Val Lys Ala Ala Phe Asp 
305                 310                 315                 320 


Ala Ala Val Ser Gly Lys Ser Val Ser Gly Asp Val Glu Leu Thr Asn 
                325                 330                 335     


Ile Ile Lys Asn Ser Ser Phe Lys Ala Val Ile Tyr Gly Gly Ser Ala 
            340                 345                 350         


Lys Asp Glu Val Gln Ile Ile Asp Gly Asn Leu Gly Asp Leu Arg Asp 
        355                 360                 365             


Ile Leu Lys Lys Gly Ala Thr Phe Asn Arg Glu Thr Pro Gly Val Pro 
    370                 375                 380                 


Ile Ala Tyr Thr Thr Asn Phe Leu Lys Asp Asn Glu Leu Ala Val Ile 
385                 390                 395                 400 


Lys Asn Asn Ser Glu Tyr Ile Glu Thr Thr Ser Lys Ala Tyr Thr Asp 
                405                 410                 415     


<210>  6
<211>  639
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ActA protein

<400>  6

Met Gly Leu Asn Arg Phe Met Arg Ala Met Met Val Val Phe Ile Thr 
1               5                   10                  15      


Ala Asn Cys Ile Thr Ile Asn Pro Asp Ile Ile Phe Ala Ala Thr Asp 
            20                  25                  30          


Ser Glu Asp Ser Ser Leu Asn Thr Asp Glu Trp Glu Glu Glu Lys Thr 
        35                  40                  45              


Glu Glu Gln Pro Ser Glu Val Asn Thr Gly Pro Arg Tyr Glu Thr Ala 
    50                  55                  60                  


Arg Glu Val Ser Ser Arg Asp Ile Lys Glu Leu Glu Lys Ser Asn Lys 
65                  70                  75                  80  


Val Arg Asn Thr Asn Lys Ala Asp Leu Ile Ala Met Leu Lys Glu Lys 
                85                  90                  95      


Ala Glu Lys Gly Pro Asn Ile Asn Asn Asn Asn Ser Glu Gln Thr Glu 
            100                 105                 110         


Asn Ala Ala Ile Asn Glu Glu Ala Ser Gly Ala Asp Arg Pro Ala Ile 
        115                 120                 125             


Gln Val Glu Arg Arg His Pro Gly Leu Pro Ser Asp Ser Ala Ala Glu 
    130                 135                 140                 


Ile Lys Lys Arg Arg Lys Ala Ile Ala Ser Ser Asp Ser Glu Leu Glu 
145                 150                 155                 160 


Ser Leu Thr Tyr Pro Asp Lys Pro Thr Lys Val Asn Lys Lys Lys Val 
                165                 170                 175     


Ala Lys Glu Ser Val Ala Asp Ala Ser Glu Ser Asp Leu Asp Ser Ser 
            180                 185                 190         


Met Gln Ser Ala Asp Glu Ser Ser Pro Gln Pro Leu Lys Ala Asn Gln 
        195                 200                 205             


Gln Pro Phe Phe Pro Lys Val Phe Lys Lys Ile Lys Asp Ala Gly Lys 
    210                 215                 220                 


Trp Val Arg Asp Lys Ile Asp Glu Asn Pro Glu Val Lys Lys Ala Ile 
225                 230                 235                 240 


Val Asp Lys Ser Ala Gly Leu Ile Asp Gln Leu Leu Thr Lys Lys Lys 
                245                 250                 255     


Ser Glu Glu Val Asn Ala Ser Asp Phe Pro Pro Pro Pro Thr Asp Glu 
            260                 265                 270         


Glu Leu Arg Leu Ala Leu Pro Glu Thr Pro Met Leu Leu Gly Phe Asn 
        275                 280                 285             


Ala Pro Ala Thr Ser Glu Pro Ser Ser Phe Glu Phe Pro Pro Pro Pro 
    290                 295                 300                 


Thr Asp Glu Glu Leu Arg Leu Ala Leu Pro Glu Thr Pro Met Leu Leu 
305                 310                 315                 320 


Gly Phe Asn Ala Pro Ala Thr Ser Glu Pro Ser Ser Phe Glu Phe Pro 
                325                 330                 335     


Pro Pro Pro Thr Glu Asp Glu Leu Glu Ile Ile Arg Glu Thr Ala Ser 
            340                 345                 350         


Ser Leu Asp Ser Ser Phe Thr Arg Gly Asp Leu Ala Ser Leu Arg Asn 
        355                 360                 365             


Ala Ile Asn Arg His Ser Gln Asn Phe Ser Asp Phe Pro Pro Ile Pro 
    370                 375                 380                 


Thr Glu Glu Glu Leu Asn Gly Arg Gly Gly Arg Pro Thr Ser Glu Glu 
385                 390                 395                 400 


Phe Ser Ser Leu Asn Ser Gly Asp Phe Thr Asp Asp Glu Asn Ser Glu 
                405                 410                 415     


Thr Thr Glu Glu Glu Ile Asp Arg Leu Ala Asp Leu Arg Asp Arg Gly 
            420                 425                 430         


Thr Gly Lys His Ser Arg Asn Ala Gly Phe Leu Pro Leu Asn Pro Phe 
        435                 440                 445             


Ala Ser Ser Pro Val Pro Ser Leu Ser Pro Lys Val Ser Lys Ile Ser 
    450                 455                 460                 


Asp Arg Ala Leu Ile Ser Asp Ile Thr Lys Lys Thr Pro Phe Lys Asn 
465                 470                 475                 480 


Pro Ser Gln Pro Leu Asn Val Phe Asn Lys Lys Thr Thr Thr Lys Thr 
                485                 490                 495     


Val Thr Lys Lys Pro Thr Pro Val Lys Thr Ala Pro Lys Leu Ala Glu 
            500                 505                 510         


Leu Pro Ala Thr Lys Pro Gln Glu Thr Val Leu Arg Glu Asn Lys Thr 
        515                 520                 525             


Pro Phe Ile Glu Lys Gln Ala Glu Thr Asn Lys Gln Ser Ile Asn Met 
    530                 535                 540                 


Pro Ser Leu Pro Val Ile Gln Lys Glu Ala Thr Glu Ser Asp Lys Glu 
545                 550                 555                 560 


Glu Met Lys Pro Gln Thr Glu Glu Lys Met Val Glu Glu Ser Glu Ser 
                565                 570                 575     


Ala Asn Asn Ala Asn Gly Lys Asn Arg Ser Ala Gly Ile Glu Glu Gly 
            580                 585                 590         


Lys Leu Ile Ala Lys Ser Ala Glu Asp Glu Lys Ala Lys Glu Glu Pro 
        595                 600                 605             


Gly Asn His Thr Thr Leu Ile Leu Ala Met Leu Ala Ile Gly Val Phe 
    610                 615                 620                 


Ser Leu Gly Ala Phe Ile Lys Ile Ile Gln Leu Arg Lys Asn Asn 
625                 630                 635                 


<210>  7
<211>  1170
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  recombinant nucleotide encoding the ActA fragment

<400>  7
atgcgtgcga tgatggtggt tttcattact gccaattgca ttacgattaa ccccgacata       60

atatttgcag cgacagatag cgaagattct agtctaaaca cagatgaatg ggaagaagaa      120

aaaacagaag agcaaccaag cgaggtaaat acgggaccaa gatacgaaac tgcacgtgaa      180

gtaagttcac gtgatattaa agaactagaa aaatcgaata aagtgagaaa tacgaacaaa      240

gcagacctaa tagcaatgtt gaaagaaaaa gcagaaaaag gtccaaatat caataataac      300

aacagtgaac aaactgagaa tgcggctata aatgaagagg cttcaggagc cgaccgacca      360

gctatacaag tggagcgtcg tcatccagga ttgccatcgg atagcgcagc ggaaattaaa      420

aaaagaagga aagccatagc atcatcggat agtgagcttg aaagccttac ttatccggat      480

aaaccaacaa aagtaaataa gaaaaaagtg gcgaaagagt cagttgcgga tgcttctgaa      540

agtgacttag attctagcat gcagtcagca gatgagtctt caccacaacc tttaaaagca      600

aaccaacaac catttttccc taaagtattt aaaaaaataa aagatgcggg gaaatgggta      660

cgtgataaaa tcgacgaaaa tcctgaagta aagaaagcga ttgttgataa aagtgcaggg      720

ttaattgacc aattattaac caaaaagaaa agtgaagagg taaatgcttc ggacttcccg      780

ccaccaccta cggatgaaga gttaagactt gctttgccag agacaccaat gcttcttggt      840

tttaatgctc ctgctacatc agaaccgagc tcattcgaat ttccaccacc acctacggat      900

gaagagttaa gacttgcttt gccagagacg ccaatgcttc ttggttttaa tgctcctgct      960

acatcggaac cgagctcgtt cgaatttcca ccgcctccaa cagaagatga actagaaatc     1020

atccgggaaa cagcatcctc gctagattct agttttacaa gaggggattt agctagtttg     1080

agaaatgcta ttaatcgcca tagtcaaaat ttctctgatt tcccaccaat cccaacagaa     1140

gaagagttga acgggagagg cggtagacca                                      1170


<210>  8
<211>  669
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  recombinant nucleotide encoding the ActA fragment

<400>  8
tttatcacgt acccatttcc ccgcatcttt tattttttta aatactttag ggaaaaatgg       60

tttttgattt gcttttaaag gttgtggtgt agactcgtct gctgactgca tgctagaatc      120

taagtcactt tcagaagcat ccacaactga ctctttcgcc acttttctct tatttgcttt      180

tgttggttta tctggataag taaggctttc aagctcacta tccgacgacg ctatggcttt      240

tcttcttttt ttaatttccg ctgcgctatc cgatgacaga cctggatgac gacgctccac      300

ttgcagagtt ggtcggtcga ctcctgaagc ctcttcattt atagccacat ttcctgtttg      360

ctcaccgttg ttattattgt tattcggacc tttctctgct tttgctttca acattgctat      420

taggtctgct ttgttcgtat ttttcacttt attcgatttt tctagttcct caatatcacg      480

tgaacttact tcacgtgcag tttcgtatct tggtcccgta tttacctcgc ttggctgctc      540

ttctgttttt tcttcttccc attcatctgt gtttagactg gaatcttcgc tatctgtcgc      600

tgcaaatatt atgtcggggt taatcgtaat gcagttggca gtaatgaaaa ctaccatcat      660

cgcacgcat                                                              669


<210>  9
<211>  390
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal fragment of an ActA protein

<400>  9

Met Arg Ala Met Met Val Val Phe Ile Thr Ala Asn Cys Ile Thr Ile 
1               5                   10                  15      


Asn Pro Asp Ile Ile Phe Ala Ala Thr Asp Ser Glu Asp Ser Ser Leu 
            20                  25                  30          


Asn Thr Asp Glu Trp Glu Glu Glu Lys Thr Glu Glu Gln Pro Ser Glu 
        35                  40                  45              


Val Asn Thr Gly Pro Arg Tyr Glu Thr Ala Arg Glu Val Ser Ser Arg 
    50                  55                  60                  


Asp Ile Lys Glu Leu Glu Lys Ser Asn Lys Val Arg Asn Thr Asn Lys 
65                  70                  75                  80  


Ala Asp Leu Ile Ala Met Leu Lys Glu Lys Ala Glu Lys Gly Pro Asn 
                85                  90                  95      


Ile Asn Asn Asn Asn Ser Glu Gln Thr Glu Asn Ala Ala Ile Asn Glu 
            100                 105                 110         


Glu Ala Ser Gly Ala Asp Arg Pro Ala Ile Gln Val Glu Arg Arg His 
        115                 120                 125             


Pro Gly Leu Pro Ser Asp Ser Ala Ala Glu Ile Lys Lys Arg Arg Lys 
    130                 135                 140                 


Ala Ile Ala Ser Ser Asp Ser Glu Leu Glu Ser Leu Thr Tyr Pro Asp 
145                 150                 155                 160 


Lys Pro Thr Lys Val Asn Lys Lys Lys Val Ala Lys Glu Ser Val Ala 
                165                 170                 175     


Asp Ala Ser Glu Ser Asp Leu Asp Ser Ser Met Gln Ser Ala Asp Glu 
            180                 185                 190         


Ser Ser Pro Gln Pro Leu Lys Ala Asn Gln Gln Pro Phe Phe Pro Lys 
        195                 200                 205             


Val Phe Lys Lys Ile Lys Asp Ala Gly Lys Trp Val Arg Asp Lys Ile 
    210                 215                 220                 


Asp Glu Asn Pro Glu Val Lys Lys Ala Ile Val Asp Lys Ser Ala Gly 
225                 230                 235                 240 


Leu Ile Asp Gln Leu Leu Thr Lys Lys Lys Ser Glu Glu Val Asn Ala 
                245                 250                 255     


Ser Asp Phe Pro Pro Pro Pro Thr Asp Glu Glu Leu Arg Leu Ala Leu 
            260                 265                 270         


Pro Glu Thr Pro Met Leu Leu Gly Phe Asn Ala Pro Ala Thr Ser Glu 
        275                 280                 285             


Pro Ser Ser Phe Glu Phe Pro Pro Pro Pro Thr Asp Glu Glu Leu Arg 
    290                 295                 300                 


Leu Ala Leu Pro Glu Thr Pro Met Leu Leu Gly Phe Asn Ala Pro Ala 
305                 310                 315                 320 


Thr Ser Glu Pro Ser Ser Phe Glu Phe Pro Pro Pro Pro Thr Glu Asp 
                325                 330                 335     


Glu Leu Glu Ile Ile Arg Glu Thr Ala Ser Ser Leu Asp Ser Ser Phe 
            340                 345                 350         


Thr Arg Gly Asp Leu Ala Ser Leu Arg Asn Ala Ile Asn Arg His Ser 
        355                 360                 365             


Gln Asn Phe Ser Asp Phe Pro Pro Ile Pro Thr Glu Glu Glu Leu Asn 
    370                 375                 380                 


Gly Arg Gly Gly Arg Pro 
385                 390 


<210>  10
<211>  1170
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  recombinant nucleotide encoding a fragment of an ActA protein

<400>  10
atgcgtgcga tgatggtggt tttcattact gccaattgca ttacgattaa ccccgacata       60

atatttgcag cgacagatag cgaagattct agtctaaaca cagatgaatg ggaagaagaa      120

aaaacagaag agcaaccaag cgaggtaaat acgggaccaa gatacgaaac tgcacgtgaa      180

gtaagttcac gtgatattaa agaactagaa aaatcgaata aagtgagaaa tacgaacaaa      240

gcagacctaa tagcaatgtt gaaagaaaaa gcagaaaaag gtccaaatat caataataac      300

aacagtgaac aaactgagaa tgcggctata aatgaagagg cttcaggagc cgaccgacca      360

gctatacaag tggagcgtcg tcatccagga ttgccatcgg atagcgcagc ggaaattaaa      420

aaaagaagga aagccatagc atcatcggat agtgagcttg aaagccttac ttatccggat      480

aaaccaacaa aagtaaataa gaaaaaagtg gcgaaagagt cagttgcgga tgcttctgaa      540

agtgacttag attctagcat gcagtcagca gatgagtctt caccacaacc tttaaaagca      600

aaccaacaac catttttccc taaagtattt aaaaaaataa aagatgcggg gaaatgggta      660

cgtgataaaa tcgacgaaaa tcctgaagta aagaaagcga ttgttgataa aagtgcaggg      720

ttaattgacc aattattaac caaaaagaaa agtgaagagg taaatgcttc ggacttcccg      780

ccaccaccta cggatgaaga gttaagactt gctttgccag agacaccaat gcttcttggt      840

tttaatgctc ctgctacatc agaaccgagc tcattcgaat ttccaccacc acctacggat      900

gaagagttaa gacttgcttt gccagagacg ccaatgcttc ttggttttaa tgctcctgct      960

acatcggaac cgagctcgtt cgaatttcca ccgcctccaa cagaagatga actagaaatc     1020

atccgggaaa cagcatcctc gctagattct agttttacaa gaggggattt agctagtttg     1080

agaaatgcta ttaatcgcca tagtcaaaat ttctctgatt tcccaccaat cccaacagaa     1140

gaagagttga acgggagagg cggtagacca                                      1170


<210>  11
<211>  100
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  truncated ActA protein

<400>  11

Met Gly Leu Asn Arg Phe Met Arg Ala Met Met Val Val Phe Ile Thr 
1               5                   10                  15      


Ala Asn Cys Ile Thr Ile Asn Pro Asp Ile Ile Phe Ala Ala Thr Asp 
            20                  25                  30          


Ser Glu Asp Ser Ser Leu Asn Thr Asp Glu Trp Glu Glu Glu Lys Thr 
        35                  40                  45              


Glu Glu Gln Pro Ser Glu Val Asn Thr Gly Pro Arg Tyr Glu Thr Ala 
    50                  55                  60                  


Arg Glu Val Ser Ser Arg Asp Ile Lys Glu Leu Glu Lys Ser Asn Lys 
65                  70                  75                  80  


Val Arg Asn Thr Asn Lys Ala Asp Leu Ile Ala Met Leu Lys Glu Lys 
                85                  90                  95      


Ala Glu Lys Gly 
            100 


<210>  12
<211>  639
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ActA protein

<400>  12

Met Gly Leu Asn Arg Phe Met Arg Ala Met Met Val Val Phe Ile Thr 
1               5                   10                  15      


Ala Asn Cys Ile Thr Ile Asn Pro Asp Ile Ile Phe Ala Ala Thr Asp 
            20                  25                  30          


Ser Glu Asp Ser Ser Leu Asn Thr Asp Glu Trp Glu Glu Glu Lys Thr 
        35                  40                  45              


Glu Glu Gln Pro Ser Glu Val Asn Thr Gly Pro Arg Tyr Glu Thr Ala 
    50                  55                  60                  


Arg Glu Val Ser Ser Arg Asp Ile Glu Glu Leu Glu Lys Ser Asn Lys 
65                  70                  75                  80  


Val Lys Asn Thr Asn Lys Ala Asp Leu Ile Ala Met Leu Lys Ala Lys 
                85                  90                  95      


Ala Glu Lys Gly Pro Asn Asn Asn Asn Asn Asn Gly Glu Gln Thr Gly 
            100                 105                 110         


Asn Val Ala Ile Asn Glu Glu Ala Ser Gly Val Asp Arg Pro Thr Leu 
        115                 120                 125             


Gln Val Glu Arg Arg His Pro Gly Leu Ser Ser Asp Ser Ala Ala Glu 
    130                 135                 140                 


Ile Lys Lys Arg Arg Lys Ala Ile Ala Ser Ser Asp Ser Glu Leu Glu 
145                 150                 155                 160 


Ser Leu Thr Tyr Pro Asp Lys Pro Thr Lys Ala Asn Lys Arg Lys Val 
                165                 170                 175     


Ala Lys Glu Ser Val Val Asp Ala Ser Glu Ser Asp Leu Asp Ser Ser 
            180                 185                 190         


Met Gln Ser Ala Asp Glu Ser Thr Pro Gln Pro Leu Lys Ala Asn Gln 
        195                 200                 205             


Lys Pro Phe Phe Pro Lys Val Phe Lys Lys Ile Lys Asp Ala Gly Lys 
    210                 215                 220                 


Trp Val Arg Asp Lys Ile Asp Glu Asn Pro Glu Val Lys Lys Ala Ile 
225                 230                 235                 240 


Val Asp Lys Ser Ala Gly Leu Ile Asp Gln Leu Leu Thr Lys Lys Lys 
                245                 250                 255     


Ser Glu Glu Val Asn Ala Ser Asp Phe Pro Pro Pro Pro Thr Asp Glu 
            260                 265                 270         


Glu Leu Arg Leu Ala Leu Pro Glu Thr Pro Met Leu Leu Gly Phe Asn 
        275                 280                 285             


Ala Pro Thr Pro Ser Glu Pro Ser Ser Phe Glu Phe Pro Pro Pro Pro 
    290                 295                 300                 


Thr Asp Glu Glu Leu Arg Leu Ala Leu Pro Glu Thr Pro Met Leu Leu 
305                 310                 315                 320 


Gly Phe Asn Ala Pro Ala Thr Ser Glu Pro Ser Ser Phe Glu Phe Pro 
                325                 330                 335     


Pro Pro Pro Thr Glu Asp Glu Leu Glu Ile Met Arg Glu Thr Ala Pro 
            340                 345                 350         


Ser Leu Asp Ser Ser Phe Thr Ser Gly Asp Leu Ala Ser Leu Arg Ser 
        355                 360                 365             


Ala Ile Asn Arg His Ser Glu Asn Phe Ser Asp Phe Pro Pro Ile Pro 
    370                 375                 380                 


Thr Glu Glu Glu Leu Asn Gly Arg Gly Gly Arg Pro Thr Ser Glu Glu 
385                 390                 395                 400 


Phe Ser Ser Leu Asn Ser Gly Asp Phe Thr Asp Asp Glu Asn Ser Glu 
                405                 410                 415     


Thr Thr Glu Glu Glu Ile Asp Arg Leu Ala Asp Leu Arg Asp Arg Gly 
            420                 425                 430         


Thr Gly Lys His Ser Arg Asn Ala Gly Phe Leu Pro Leu Asn Pro Phe 
        435                 440                 445             


Ile Ser Ser Pro Val Pro Ser Leu Thr Pro Lys Val Pro Lys Ile Ser 
    450                 455                 460                 


Ala Pro Ala Leu Ile Ser Asp Ile Thr Lys Lys Ala Pro Phe Lys Asn 
465                 470                 475                 480 


Pro Ser Gln Pro Leu Asn Val Phe Asn Lys Lys Thr Thr Thr Lys Thr 
                485                 490                 495     


Val Thr Lys Lys Pro Thr Pro Val Lys Thr Ala Pro Lys Leu Ala Glu 
            500                 505                 510         


Leu Pro Ala Thr Lys Pro Gln Glu Thr Val Leu Arg Glu Asn Lys Thr 
        515                 520                 525             


Pro Phe Ile Glu Lys Gln Ala Glu Thr Asn Lys Gln Ser Ile Asn Met 
    530                 535                 540                 


Pro Ser Leu Pro Val Ile Gln Lys Glu Ala Thr Glu Ser Asp Lys Glu 
545                 550                 555                 560 


Glu Met Lys Pro Gln Thr Glu Glu Lys Met Val Glu Glu Ser Glu Ser 
                565                 570                 575     


Ala Asn Asn Ala Asn Gly Lys Asn Arg Ser Ala Gly Ile Glu Glu Gly 
            580                 585                 590         


Lys Leu Ile Ala Lys Ser Ala Glu Asp Glu Lys Ala Lys Glu Glu Pro 
        595                 600                 605             


Gly Asn His Thr Thr Leu Ile Leu Ala Met Leu Ala Ile Gly Val Phe 
    610                 615                 620                 


Ser Leu Gly Ala Phe Ile Lys Ile Ile Gln Leu Arg Lys Asn Asn 
625                 630                 635                 


<210>  13
<211>  93
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  truncated ActA protein

<400>  13

Ala Thr Asp Ser Glu Asp Ser Ser Leu Asn Thr Asp Glu Trp Glu Glu 
1               5                   10                  15      


Glu Lys Thr Glu Glu Gln Pro Ser Glu Val Asn Thr Gly Pro Arg Tyr 
            20                  25                  30          


Glu Thr Ala Arg Glu Val Ser Ser Arg Asp Ile Glu Glu Leu Glu Lys 
        35                  40                  45              


Ser Asn Lys Val Lys Asn Thr Asn Lys Ala Asp Leu Ile Ala Met Leu 
    50                  55                  60                  


Lys Ala Lys Ala Glu Lys Gly Pro Asn Asn Asn Asn Asn Asn Gly Glu 
65                  70                  75                  80  


Gln Thr Gly Asn Val Ala Ile Asn Glu Glu Ala Ser Gly 
                85                  90              


<210>  14
<211>  200
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  truncated ActA protein

<400>  14

Ala Thr Asp Ser Glu Asp Ser Ser Leu Asn Thr Asp Glu Trp Glu Glu 
1               5                   10                  15      


Glu Lys Thr Glu Glu Gln Pro Ser Glu Val Asn Thr Gly Pro Arg Tyr 
            20                  25                  30          


Glu Thr Ala Arg Glu Val Ser Ser Arg Asp Ile Glu Glu Leu Glu Lys 
        35                  40                  45              


Ser Asn Lys Val Lys Asn Thr Asn Lys Ala Asp Leu Ile Ala Met Leu 
    50                  55                  60                  


Lys Ala Lys Ala Glu Lys Gly Pro Asn Asn Asn Asn Asn Asn Gly Glu 
65                  70                  75                  80  


Gln Thr Gly Asn Val Ala Ile Asn Glu Glu Ala Ser Gly Val Asp Arg 
                85                  90                  95      


Pro Thr Leu Gln Val Glu Arg Arg His Pro Gly Leu Ser Ser Asp Ser 
            100                 105                 110         


Ala Ala Glu Ile Lys Lys Arg Arg Lys Ala Ile Ala Ser Ser Asp Ser 
        115                 120                 125             


Glu Leu Glu Ser Leu Thr Tyr Pro Asp Lys Pro Thr Lys Ala Asn Lys 
    130                 135                 140                 


Arg Lys Val Ala Lys Glu Ser Val Val Asp Ala Ser Glu Ser Asp Leu 
145                 150                 155                 160 


Asp Ser Ser Met Gln Ser Ala Asp Glu Ser Thr Pro Gln Pro Leu Lys 
                165                 170                 175     


Ala Asn Gln Lys Pro Phe Phe Pro Lys Val Phe Lys Lys Ile Lys Asp 
            180                 185                 190         


Ala Gly Lys Trp Val Arg Asp Lys 
        195                 200 


<210>  15
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  truncated ActA protein

<400>  15

Ala Thr Asp Ser Glu Asp Ser Ser Leu Asn Thr Asp Glu Trp Glu Glu 
1               5                   10                  15      


Glu Lys Thr Glu Glu Gln Pro Ser Glu Val Asn Thr Gly Pro Arg Tyr 
            20                  25                  30          


Glu Thr Ala Arg Glu Val Ser Ser Arg Asp Ile Glu Glu Leu Glu Lys 
        35                  40                  45              


Ser Asn Lys Val Lys Asn Thr Asn Lys Ala Asp Leu Ile Ala Met Leu 
    50                  55                  60                  


Lys Ala Lys Ala Glu Lys Gly Pro Asn Asn Asn Asn Asn Asn Gly Glu 
65                  70                  75                  80  


Gln Thr Gly Asn Val Ala Ile Asn Glu Glu Ala Ser Gly Val Asp Arg 
                85                  90                  95      


Pro Thr Leu Gln Val Glu Arg Arg His Pro Gly Leu Ser Ser Asp Ser 
            100                 105                 110         


Ala Ala Glu Ile Lys Lys Arg Arg Lys Ala Ile Ala Ser Ser Asp Ser 
        115                 120                 125             


Glu Leu Glu Ser Leu Thr Tyr Pro Asp Lys Pro Thr Lys Ala Asn Lys 
    130                 135                 140                 


Arg Lys Val Ala Lys Glu Ser Val Val Asp Ala Ser Glu Ser Asp Leu 
145                 150                 155                 160 


Asp Ser Ser Met Gln Ser Ala Asp Glu Ser Thr Pro Gln Pro Leu Lys 
                165                 170                 175     


Ala Asn Gln Lys Pro Phe Phe Pro Lys Val Phe Lys Lys Ile Lys Asp 
            180                 185                 190         


Ala Gly Lys Trp Val Arg Asp Lys Ile Asp Glu Asn Pro Glu Val Lys 
        195                 200                 205             


Lys Ala Ile Val Asp Lys Ser Ala Gly Leu Ile Asp Gln Leu Leu Thr 
    210                 215                 220                 


Lys Lys Lys Ser Glu Glu Val Asn Ala Ser Asp Phe Pro Pro Pro Pro 
225                 230                 235                 240 


Thr Asp Glu Glu Leu Arg Leu Ala Leu Pro Glu Thr Pro Met Leu Leu 
                245                 250                 255     


Gly Phe Asn Ala Pro Thr Pro Ser Glu Pro Ser Ser Phe Glu Phe Pro 
            260                 265                 270         


Pro Pro Pro Thr Asp Glu Glu Leu Arg Leu Ala Leu Pro Glu Thr Pro 
        275                 280                 285             


Met Leu Leu Gly Phe Asn Ala Pro Ala Thr Ser Glu Pro Ser Ser 
    290                 295                 300             


<210>  16
<211>  370
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  truncated ActA protein

<400>  16

Ala Thr Asp Ser Glu Asp Ser Ser Leu Asn Thr Asp Glu Trp Glu Glu 
1               5                   10                  15      


Glu Lys Thr Glu Glu Gln Pro Ser Glu Val Asn Thr Gly Pro Arg Tyr 
            20                  25                  30          


Glu Thr Ala Arg Glu Val Ser Ser Arg Asp Ile Glu Glu Leu Glu Lys 
        35                  40                  45              


Ser Asn Lys Val Lys Asn Thr Asn Lys Ala Asp Leu Ile Ala Met Leu 
    50                  55                  60                  


Lys Ala Lys Ala Glu Lys Gly Pro Asn Asn Asn Asn Asn Asn Gly Glu 
65                  70                  75                  80  


Gln Thr Gly Asn Val Ala Ile Asn Glu Glu Ala Ser Gly Val Asp Arg 
                85                  90                  95      


Pro Thr Leu Gln Val Glu Arg Arg His Pro Gly Leu Ser Ser Asp Ser 
            100                 105                 110         


Ala Ala Glu Ile Lys Lys Arg Arg Lys Ala Ile Ala Ser Ser Asp Ser 
        115                 120                 125             


Glu Leu Glu Ser Leu Thr Tyr Pro Asp Lys Pro Thr Lys Ala Asn Lys 
    130                 135                 140                 


Arg Lys Val Ala Lys Glu Ser Val Val Asp Ala Ser Glu Ser Asp Leu 
145                 150                 155                 160 


Asp Ser Ser Met Gln Ser Ala Asp Glu Ser Thr Pro Gln Pro Leu Lys 
                165                 170                 175     


Ala Asn Gln Lys Pro Phe Phe Pro Lys Val Phe Lys Lys Ile Lys Asp 
            180                 185                 190         


Ala Gly Lys Trp Val Arg Asp Lys Ile Asp Glu Asn Pro Glu Val Lys 
        195                 200                 205             


Lys Ala Ile Val Asp Lys Ser Ala Gly Leu Ile Asp Gln Leu Leu Thr 
    210                 215                 220                 


Lys Lys Lys Ser Glu Glu Val Asn Ala Ser Asp Phe Pro Pro Pro Pro 
225                 230                 235                 240 


Thr Asp Glu Glu Leu Arg Leu Ala Leu Pro Glu Thr Pro Met Leu Leu 
                245                 250                 255     


Gly Phe Asn Ala Pro Thr Pro Ser Glu Pro Ser Ser Phe Glu Phe Pro 
            260                 265                 270         


Pro Pro Pro Thr Asp Glu Glu Leu Arg Leu Ala Leu Pro Glu Thr Pro 
        275                 280                 285             


Met Leu Leu Gly Phe Asn Ala Pro Ala Thr Ser Glu Pro Ser Ser Phe 
    290                 295                 300                 


Glu Phe Pro Pro Pro Pro Thr Glu Asp Glu Leu Glu Ile Met Arg Glu 
305                 310                 315                 320 


Thr Ala Pro Ser Leu Asp Ser Ser Phe Thr Ser Gly Asp Leu Ala Ser 
                325                 330                 335     


Leu Arg Ser Ala Ile Asn Arg His Ser Glu Asn Phe Ser Asp Phe Pro 
            340                 345                 350         


Leu Ile Pro Thr Glu Glu Glu Leu Asn Gly Arg Gly Gly Arg Pro Thr 
        355                 360                 365             


Ser Glu 
    370 


<210>  17
<211>  226
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  truncated ActA fused to hly signal peptide

<400>  17

Met Lys Lys Ile Met Leu Val Phe Ile Thr Leu Ile Leu Val Ser Leu 
1               5                   10                  15      


Pro Ile Ala Gln Gln Thr Glu Ala Ser Arg Ala Thr Asp Ser Glu Asp 
            20                  25                  30          


Ser Ser Leu Asn Thr Asp Glu Trp Glu Glu Glu Lys Thr Glu Glu Gln 
        35                  40                  45              


Pro Ser Glu Val Asn Thr Gly Pro Arg Tyr Glu Thr Ala Arg Glu Val 
    50                  55                  60                  


Ser Ser Arg Asp Ile Glu Glu Leu Glu Lys Ser Asn Lys Val Lys Asn 
65                  70                  75                  80  


Thr Asn Lys Ala Asp Leu Ile Ala Met Leu Lys Ala Lys Ala Glu Lys 
                85                  90                  95      


Gly Pro Asn Asn Asn Asn Asn Asn Gly Glu Gln Thr Gly Asn Val Ala 
            100                 105                 110         


Ile Asn Glu Glu Ala Ser Gly Val Asp Arg Pro Thr Leu Gln Val Glu 
        115                 120                 125             


Arg Arg His Pro Gly Leu Ser Ser Asp Ser Ala Ala Glu Ile Lys Lys 
    130                 135                 140                 


Arg Arg Lys Ala Ile Ala Ser Ser Asp Ser Glu Leu Glu Ser Leu Thr 
145                 150                 155                 160 


Tyr Pro Asp Lys Pro Thr Lys Ala Asn Lys Arg Lys Val Ala Lys Glu 
                165                 170                 175     


Ser Val Val Asp Ala Ser Glu Ser Asp Leu Asp Ser Ser Met Gln Ser 
            180                 185                 190         


Ala Asp Glu Ser Thr Pro Gln Pro Leu Lys Ala Asn Gln Lys Pro Phe 
        195                 200                 205             


Phe Pro Lys Val Phe Lys Lys Ile Lys Asp Ala Gly Lys Trp Val Arg 
    210                 215                 220                 


Asp Lys 
225     


<210>  18
<211>  678
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  recombinant nucleotide encoding a truncated ActA fused to hly 
       signal peptide

<400>  18
atgaaaaaaa taatgctagt ttttattaca cttatattag ttagtctacc aattgcgcaa       60

caaactgaag catctagagc gacagatagc gaagattcca gtctaaacac agatgaatgg      120

gaagaagaaa aaacagaaga gcagccaagc gaggtaaata cgggaccaag atacgaaact      180

gcacgtgaag taagttcacg tgatattgag gaactagaaa aatcgaataa agtgaaaaat      240

acgaacaaag cagacctaat agcaatgttg aaagcaaaag cagagaaagg tccgaataac      300

aataataaca acggtgagca aacaggaaat gtggctataa atgaagaggc ttcaggagtc      360

gaccgaccaa ctctgcaagt ggagcgtcgt catccaggtc tgtcatcgga tagcgcagcg      420

gaaattaaaa aaagaagaaa agccatagcg tcgtcggata gtgagcttga aagccttact      480

tatccagata aaccaacaaa agcaaataag agaaaagtgg cgaaagagtc agttgtggat      540

gcttctgaaa gtgacttaga ttctagcatg cagtcagcag acgagtctac accacaacct      600

ttaaaagcaa atcaaaaacc atttttccct aaagtattta aaaaaataaa agatgcgggg      660

aaatgggtac gtgataaa                                                    678


<210>  19
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PEST sequence

<400>  19

Lys Glu Asn Ser Ile Ser Ser Met Ala Pro Pro Ala Ser Pro Pro Ala 
1               5                   10                  15      


Ser Pro Lys Thr Pro Ile Glu Lys Lys His Ala Asp Glu Ile Asp Lys 
            20                  25                  30          


<210>  20
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PEST sequence

<400>  20

Lys Glu Asn Ser Ile Ser Ser Met Ala Pro Pro Ala Ser Pro Pro Ala 
1               5                   10                  15      


Ser Pro Lys 
            


<210>  21
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PEST sequence

<400>  21

Lys Thr Glu Glu Gln Pro Ser Glu Val Asn Thr Gly Pro Arg 
1               5                   10                  


<210>  22
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PEST sequence

<400>  22

Lys Ala Ser Val Thr Asp Thr Ser Glu Gly Asp Leu Asp Ser Ser Met 
1               5                   10                  15      


Gln Ser Ala Asp Glu Ser Thr Pro Gln Pro Leu Lys 
            20                  25              


<210>  23
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PEST sequence

<400>  23

Lys Asn Glu Glu Val Asn Ala Ser Asp Phe Pro Pro Pro Pro Thr Asp 
1               5                   10                  15      


Glu Glu Leu Arg 
            20  


<210>  24
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PEST sequence

<400>  24

Arg Gly Gly Ile Pro Thr Ser Glu Glu Phe Ser Ser Leu Asn Ser Gly 
1               5                   10                  15      


Asp Phe Thr Asp Asp Glu Asn Ser Glu Thr Thr Glu Glu Glu Ile Asp 
            20                  25                  30          


Arg 
    


<210>  25
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PEST sequence

<400>  25

Lys Glu Ser Val Val Asp Ala Ser Glu Ser Asp Leu Asp Ser Ser Met 
1               5                   10                  15      


Gln Ser Ala Asp Glu Ser Thr Pro Gln Pro Leu Lys 
            20                  25              


<210>  26
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PEST sequence

<400>  26

Lys Ser Glu Glu Val Asn Ala Ser Asp Phe Pro Pro Pro Pro Thr Asp 
1               5                   10                  15      


Glu Glu Leu Arg 
            20  


<210>  27
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PEST sequence

<400>  27

Arg Gly Gly Arg Pro Thr Ser Glu Glu Phe Ser Ser Leu Asn Ser Gly 
1               5                   10                  15      


Asp Phe Thr Asp Asp Glu Asn Ser Glu Thr Thr Glu Glu Glu Ile Asp 
            20                  25                  30          


Arg 
    


<210>  28
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PEST sequence

<400>  28

Arg Ser Glu Val Thr Ile Ser Pro Ala Glu Thr Pro Glu Ser Pro Pro 
1               5                   10                  15      


Ala Thr Pro 
            


<210>  29
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PEST sequence

<400>  29

Lys Gln Asn Thr Ala Ser Thr Glu Thr Thr Thr Thr Asn Glu Gln Pro 
1               5                   10                  15      


Lys 
    


<210>  30
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PEST sequence

<400>  30

Lys Gln Asn Thr Ala Asn Thr Glu Thr Thr Thr Thr Asn Glu Gln Pro 
1               5                   10                  15      


Lys 
    


<210>  31
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  31
ccatggtgac aggctggcat c                                                 21


<210>  32
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  32
gctagcctaa tggatgtatt ttctagg                                           27


<210>  33
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  33
ttaattaaca aatagttggt atagtcc                                           27


<210>  34
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  34
gacgatgcca gcctgtcacc atggaaaact cctctc                                 36


<210>  35
<211>  156
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  truncated p60 promoter

<400>  35
caaatagttg gtatagtcct ctttagcctt tggagtatta tctcatcatt tgttttttag       60

gtgaaaactg ggtaaactta gtattatcaa tataaaatta attctcaaat acttaattac      120

gtactgggat tttctgaaaa aagagaggag ttttcc                                156


<210>  36
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  36
ggcgccacta actcaacgct agtag                                             25


<210>  37
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  37
gctagccagc aaagaaaaac aaacacg                                           27


<210>  38
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  38
gtcgacggtc accggcgcca ctaactcaac gctagtag                               38


<210>  39
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  39
ttaattaagc tagccagcaa agaaaaacaa acacg                                  35


<210>  40
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  40
atgaaaaaaa taatgctagt ttttattac                                         29


<210>  41
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  41
gcggccgctt aatgatgatg atgatgatgt ggtttctgag aacagatg                    48


<210>  42
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  42
gcaagtgtga ctctacgctt cg                                                22


<210>  43
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  43
tgcccattaa caggtcttcc a                                                 21


<210>  44
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  44
tgcgtacaaa gcacacacgt agacattcgt ac                                     32


<210>  45
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  45
tgacatcgtt tgtgtttgag ctag                                              24


<210>  46
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  46
gcagcgctct ctataccagg tac                                               23


<210>  47
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  47
ttaatgtcca tgttatgtct ccgttatagc tcatcgta                               38


<210>  48
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  48
tgggatggcc aagaaattc                                                    19


<210>  49
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  49
ctaccatgtc ttccgttgct tg                                                22


<210>  50
<211>  1256
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lm-dd?actA

<400>  50
gcgccaaatc attggttgat tggtgaggat gtctgtgtgc gtgggtcgcg agatgggcga       60

ataagaagca ttaaagatcc tgacaaatat aatcaagcgg ctcatatgaa agattacgaa      120

tcgcttccac tcacagagga aggcgactgg ggcggagttc attataatag tggtatcccg      180

aataaagcag cctataatac tatcactaaa cttggaaaag aaaaaacaga acagctttat      240

tttcgcgcct taaagtacta tttaacgaaa aaatcccagt ttaccgatgc gaaaaaagcg      300

cttcaacaag cagcgaaaga tttatatggt gaagatgctt ctaaaaaagt tgctgaagct      360

tgggaagcag ttggggttaa ctgattaaca aatgttagag aaaaattaat tctccaagtg      420

atattcttaa aataattcat gaatattttt tcttatatta gctaattaag aagataacta      480

actgctaatc caatttttaa cggaacaaat tagtgaaaat gaaggccgaa ttttccttgt      540

tctaaaaagg ttgtattagc gtatcacgag gagggagtat aagtgggatt aaacagattt      600

atgcgtgcga tgatggtggt tttcattact gccaattgca ttacgattaa ccccgacgtc      660

gacccatacg acgttaattc ttgcaatgtt agctattggc gtgttctctt taggggcgtt      720

tatcaaaatt attcaattaa gaaaaaataa ttaaaaacac agaacgaaag aaaaagtgag      780

gtgaatgata tgaaattcaa aaaggtggtt ctaggtatgt gcttgatcgc aagtgttcta      840

gtctttccgg taacgataaa agcaaatgcc tgttgtgatg aatacttaca aacacccgca      900

gctccgcatg atattgacag caaattacca cataaactta gttggtccgc ggataacccg      960

acaaatactg acgtaaatac gcactattgg ctttttaaac aagcggaaaa aatactagct     1020

aaagatgtaa atcatatgcg agctaattta atgaatgaac ttaaaaaatt cgataaacaa     1080

atagctcaag gaatatatga tgcggatcat aaaaatccat attatgatac tagtacattt     1140

ttatctcatt tttataatcc tgatagagat aatacttatt tgccgggttt tgctaatgcg     1200

aaaataacag gagcaaagta tttcaatcaa tcggtgactg attaccgaga agggaa         1256


<210>  51
<211>  6523
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  plasmid pAdv142 (6523 bp)

<400>  51
cggagtgtat actggcttac tatgttggca ctgatgaggg tgtcagtgaa gtgcttcatg       60

tggcaggaga aaaaaggctg caccggtgcg tcagcagaat atgtgataca ggatatattc      120

cgcttcctcg ctcactgact cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc      180

ttacgaacgg ggcggagatt tcctggaaga tgccaggaag atacttaaca gggaagtgag      240

agggccgcgg caaagccgtt tttccatagg ctccgccccc ctgacaagca tcacgaaatc      300

tgacgctcaa atcagtggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc      360

cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg      420

ctgttatggc cgcgtttgtc tcattccacg cctgacactc agttccgggt aggcagttcg      480

ctccaagctg gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg      540

taactatcgt cttgagtcca acccggaaag acatgcaaaa gcaccactgg cagcagccac      600

tggtaattga tttagaggag ttagtcttga agtcatgcgc cggttaaggc taaactgaaa      660

ggacaagttt tggtgactgc gctcctccaa gccagttacc tcggttcaaa gagttggtag      720

ctcagagaac cttcgaaaaa ccgccctgca aggcggtttt ttcgttttca gagcaagaga      780

ttacgcgcag accaaaacga tctcaagaag atcatcttat taatcagata aaatatttct      840

agccctcctt tgattagtat attcctatct taaagttact tttatgtgga ggcattaaca      900

tttgttaatg acgtcaaaag gatagcaaga ctagaataaa gctataaagc aagcatataa      960

tattgcgttt catctttaga agcgaatttc gccaatatta taattatcaa aagagagggg     1020

tggcaaacgg tatttggcat tattaggtta aaaaatgtag aaggagagtg aaacccatga     1080

aaaaaataat gctagttttt attacactta tattagttag tctaccaatt gcgcaacaaa     1140

ctgaagcaaa ggatgcatct gcattcaata aagaaaattc aatttcatcc atggcaccac     1200

cagcatctcc gcctgcaagt cctaagacgc caatcgaaaa gaaacacgcg gatgaaatcg     1260

ataagtatat acaaggattg gattacaata aaaacaatgt attagtatac cacggagatg     1320

cagtgacaaa tgtgccgcca agaaaaggtt acaaagatgg aaatgaatat attgttgtgg     1380

agaaaaagaa gaaatccatc aatcaaaata atgcagacat tcaagttgtg aatgcaattt     1440

cgagcctaac ctatccaggt gctctcgtaa aagcgaattc ggaattagta gaaaatcaac     1500

cagatgttct ccctgtaaaa cgtgattcat taacactcag cattgatttg ccaggtatga     1560

ctaatcaaga caataaaata gttgtaaaaa atgccactaa atcaaacgtt aacaacgcag     1620

taaatacatt agtggaaaga tggaatgaaa aatatgctca agcttatcca aatgtaagtg     1680

caaaaattga ttatgatgac gaaatggctt acagtgaatc acaattaatt gcgaaatttg     1740

gtacagcatt taaagctgta aataatagct tgaatgtaaa cttcggcgca atcagtgaag     1800

ggaaaatgca agaagaagtc attagtttta aacaaattta ctataacgtg aatgttaatg     1860

aacctacaag accttccaga tttttcggca aagctgttac taaagagcag ttgcaagcgc     1920

ttggagtgaa tgcagaaaat cctcctgcat atatctcaag tgtggcgtat ggccgtcaag     1980

tttatttgaa attatcaact aattcccata gtactaaagt aaaagctgct tttgatgctg     2040

ccgtaagcgg aaaatctgtc tcaggtgatg tagaactaac aaatatcatc aaaaattctt     2100

ccttcaaagc cgtaatttac ggaggttccg caaaagatga agttcaaatc atcgacggca     2160

acctcggaga cttacgcgat attttgaaaa aaggcgctac ttttaatcga gaaacaccag     2220

gagttcccat tgcttataca acaaacttcc taaaagacaa tgaattagct gttattaaaa     2280

acaactcaga atatattgaa acaacttcaa aagcttatac agatggaaaa attaacatcg     2340

atcactctgg aggatacgtt gctcaattca acatttcttg ggatgaagta aattatgatc     2400

tcgagattgt gggaggctgg gagtgcgaga agcattccca accctggcag gtgcttgtgg     2460

cctctcgtgg cagggcagtc tgcggcggtg ttctggtgca cccccagtgg gtcctcacag     2520

ctgcccactg catcaggaac aaaagcgtga tcttgctggg tcggcacagc ctgtttcatc     2580

ctgaagacac aggccaggta tttcaggtca gccacagctt cccacacccg ctctacgata     2640

tgagcctcct gaagaatcga ttcctcaggc caggtgatga ctccagccac gacctcatgc     2700

tgctccgcct gtcagagcct gccgagctca cggatgctgt gaaggtcatg gacctgccca     2760

cccaggagcc agcactgggg accacctgct acgcctcagg ctggggcagc attgaaccag     2820

aggagttctt gaccccaaag aaacttcagt gtgtggacct ccatgttatt tccaatgacg     2880

tgtgtgcgca agttcaccct cagaaggtga ccaagttcat gctgtgtgct ggacgctgga     2940

cagggggcaa aagcacctgc tcgggtgatt ctgggggccc acttgtctgt tatggtgtgc     3000

ttcaaggtat cacgtcatgg ggcagtgaac catgtgccct gcccgaaagg ccttccctgt     3060

acaccaaggt ggtgcattac cggaagtgga tcaaggacac catcgtggcc aacccctaac     3120

ccgggccact aactcaacgc tagtagtgga tttaatccca aatgagccaa cagaaccaga     3180

accagaaaca gaacaagtaa cattggagtt agaaatggaa gaagaaaaaa gcaatgattt     3240

cgtgtgaata atgcacgaaa tcattgctta tttttttaaa aagcgatata ctagatataa     3300

cgaaacaacg aactgaataa agaatacaaa aaaagagcca cgaccagtta aagcctgaga     3360

aactttaact gcgagcctta attgattacc accaatcaat taaagaagtc gagacccaaa     3420

atttggtaaa gtatttaatt actttattaa tcagatactt aaatatctgt aaacccatta     3480

tatcgggttt ttgaggggat ttcaagtctt taagaagata ccaggcaatc aattaagaaa     3540

aacttagttg attgcctttt ttgttgtgat tcaactttga tcgtagcttc taactaatta     3600

attttcgtaa gaaaggagaa cagctgaatg aatatccctt ttgttgtaga aactgtgctt     3660

catgacggct tgttaaagta caaatttaaa aatagtaaaa ttcgctcaat cactaccaag     3720

ccaggtaaaa gtaaaggggc tatttttgcg tatcgctcaa aaaaaagcat gattggcgga     3780

cgtggcgttg ttctgacttc cgaagaagcg attcacgaaa atcaagatac atttacgcat     3840

tggacaccaa acgtttatcg ttatggtacg tatgcagacg aaaaccgttc atacactaaa     3900

ggacattctg aaaacaattt aagacaaatc aataccttct ttattgattt tgatattcac     3960

acggaaaaag aaactatttc agcaagcgat attttaacaa cagctattga tttaggtttt     4020

atgcctacgt taattatcaa atctgataaa ggttatcaag catattttgt tttagaaacg     4080

ccagtctatg tgacttcaaa atcagaattt aaatctgtca aagcagccaa aataatctcg     4140

caaaatatcc gagaatattt tggaaagtct ttgccagttg atctaacgtg caatcatttt     4200

gggattgctc gtataccaag aacggacaat gtagaatttt ttgatcccaa ttaccgttat     4260

tctttcaaag aatggcaaga ttggtctttc aaacaaacag ataataaggg ctttactcgt     4320

tcaagtctaa cggttttaag cggtacagaa ggcaaaaaac aagtagatga accctggttt     4380

aatctcttat tgcacgaaac gaaattttca ggagaaaagg gtttagtagg gcgcaatagc     4440

gttatgttta ccctctcttt agcctacttt agttcaggct attcaatcga aacgtgcgaa     4500

tataatatgt ttgagtttaa taatcgatta gatcaaccct tagaagaaaa agaagtaatc     4560

aaaattgtta gaagtgccta ttcagaaaac tatcaagggg ctaataggga atacattacc     4620

attctttgca aagcttgggt atcaagtgat ttaaccagta aagatttatt tgtccgtcaa     4680

gggtggttta aattcaagaa aaaaagaagc gaacgtcaac gtgttcattt gtcagaatgg     4740

aaagaagatt taatggctta tattagcgaa aaaagcgatg tatacaagcc ttatttagcg     4800

acgaccaaaa aagagattag agaagtgcta ggcattcctg aacggacatt agataaattg     4860

ctgaaggtac tgaaggcgaa tcaggaaatt ttctttaaga ttaaaccagg aagaaatggt     4920

ggcattcaac ttgctagtgt taaatcattg ttgctatcga tcattaaatt aaaaaaagaa     4980

gaacgagaaa gctatataaa ggcgctgaca gcttcgttta atttagaacg tacatttatt     5040

caagaaactc taaacaaatt ggcagaacgc cccaaaacgg acccacaact cgatttgttt     5100

agctacgata caggctgaaa ataaaacccg cactatgcca ttacatttat atctatgata     5160

cgtgtttgtt tttctttgct ggctagctta attgcttata tttacctgca ataaaggatt     5220

tcttacttcc attatactcc cattttccaa aaacatacgg ggaacacggg aacttattgt     5280

acaggccacc tcatagttaa tggtttcgag ccttcctgca atctcatcca tggaaatata     5340

ttcatccccc tgccggccta ttaatgtgac ttttgtgccc ggcggatatt cctgatccag     5400

ctccaccata aattggtcca tgcaaattcg gccggcaatt ttcaggcgtt ttcccttcac     5460

aaggatgtcg gtccctttca attttcggag ccagccgtcc gcatagccta caggcaccgt     5520

cccgatccat gtgtcttttt ccgctgtgta ctcggctccg tagctgacgc tctcgccttt     5580

tctgatcagt ttgacatgtg acagtgtcga atgcagggta aatgccggac gcagctgaaa     5640

cggtatctcg tccgacatgt cagcagacgg gcgaaggcca tacatgccga tgccgaatct     5700

gactgcatta aaaaagcctt ttttcagccg gagtccagcg gcgctgttcg cgcagtggac     5760

cattagattc tttaacggca gcggagcaat cagctcttta aagcgctcaa actgcattaa     5820

gaaatagcct ctttcttttt catccgctgt cgcaaaatgg gtaaataccc ctttgcactt     5880

taaacgaggg ttgcggtcaa gaattgccat cacgttctga acttcttcct ctgtttttac     5940

accaagtctg ttcatccccg tatcgacctt cagatgaaaa tgaagagaac cttttttcgt     6000

gtggcgggct gcctcctgaa gccattcaac agaataacct gttaaggtca cgtcatactc     6060

agcagcgatt gccacatact ccgggggaac cgcgccaagc accaatatag gcgccttcaa     6120

tccctttttg cgcagtgaaa tcgcttcatc caaaatggcc acggccaagc atgaagcacc     6180

tgcgtcaaga gcagcctttg ctgtttctgc atcaccatgc ccgtaggcgt ttgctttcac     6240

aactgccatc aagtggacat gttcaccgat atgttttttc atattgctga cattttcctt     6300

tatcgcggac aagtcaattt ccgcccacgt atctctgtaa aaaggttttg tgctcatgga     6360

aaactcctct cttttttcag aaaatcccag tacgtaatta agtatttgag aattaatttt     6420

atattgatta atactaagtt tacccagttt tcacctaaaa aacaaatgat gagataatag     6480

ctccaaaggc taaagaggac tataccaact atttgttaat taa                       6523


