                         SEQUENCE LISTING

<110>  EnviroLogix Inc.
 
<120>  SUBSTRATE MOLECULE

<130>  049224-1058WO1

<140>  PCT/US16/14753
<141>  2016-01-25

<150>  62/110,237
<151>  2015-01-30

<160>  46    

<170>  PatentIn version 3.5

<210>  1
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<223>  3'-FAM-Modified

<400>  1
attacagagt cactgccacg gagtgcatcg cgcgggcc                               38


<210>  2
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<223>  5'-Quencher-Modified

<400>  2
ggcccgcgcg atgcactccg tggcagtgac tctgtaat                               38


<210>  3
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<223>  3'-FAM-Modified

<400>  3
ccacggagtg catcgcgcgg gcc                                               23


<210>  4
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  4
attacagagt cactgccacg ga                                                22


<210>  5
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  5
attacagagt cactgccacg gagtgcatcg cgcgggcc                               38


<210>  6
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<223>  5'-5IAbRQ-Modified

<400>  6
ggcccgcgcg atgcactccg tggcagtgac tctgtaat                               38


<210>  7
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<223>  3'-36-FAM-Modified

<400>  7
attacagagt cactgccacg gagtgcatcg cgcgggcc                               38


<210>  8
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  modified_base
<222>  (14)..(14)
<223>  2'-o-methyl nucleotide


<220>
<223>  3'-36-FAM-Modified

<400>  8
attacagagt cacugccacg gagtgcatcg cgcgggcc                               38


<210>  9
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  modified_base
<222>  (16)..(16)
<223>  2'-o-methyl nucleotide

<220>
<223>  3'-36-FAM-Modified

<400>  9
attacagagt cactgccacg gagtgcatcg cgcgggcc                               38


<210>  10
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  modified_base
<222>  (15)..(15)
<223>  2'-o-methyl nucleotide


<220>
<223>  3'-36-FAM-Modified

<400>  10
attacagagt cactgccacg gagtgcatcg cgcgggcc                               38


<210>  11
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<223>  3'-36-FAM-Modified

<400>  11
tacagagtca ctgccacgga gtgcatcgcg cgggcc                                 36


<210>  12
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<223>  3'-36-FAM-Modified

<400>  12
acagagtcac tgccacggag tgcatcgcgc gggcc                                  35


<210>  13
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<223>  3'-36-FAM-Modified

<400>  13
cagagtcact gccacggagt gcatcgcgcg ggcc                                   34


<210>  14
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<223>  3'-36-FAM-Modified

<400>  14
agagtcactg ccacggagtg catcgcgcgg gcc                                    33


<210>  15
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<223>  3'-36-FAM-Modified

<400>  15
gagtcactgc cacggagtgc atcgcgcggg cc                                     32


<210>  16
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  modified_base
<222>  (15)..(15)
<223>  2'-o-methyl nucleotide

<220>
<221>  modified_base
<222>  (16)..(16)
<223>  2'-o-methyl nucleotide


<220>
<223>  3'-36-FAM-Modified

<400>  16
attacagagt cactgccacg gagtgcatcg cgcgggcc                               38


<210>  17
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<223>  5'-IAbRQ-Modified

<220>
<223>  3'-C3 Spacer-Modified

<400>  17
ggcccgcgcg atgcactccg tggcagtgac tctgtaat                               38


<210>  18
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  modified_base
<222>  (7)..(7)
<223>  2'-o-methyl nucleotide


<220>
<223>  3'-36-FAM-Modified

<400>  18
attacagagt cactgccacg gagtgcatcg cgcgggcc                               38


<210>  19
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  modified_base
<222>  (8)..(8)
<223>  2'-o-methyl nucleotide


<220>
<223>  3'-36-FAM-Modified

<400>  19
attacagagt cactgccacg gagtgcatcg cgcgggcc                               38


<210>  20
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  modified_base
<222>  (9)..(9)
<223>  2'-o-methyl nucleotide


<220>
<223>  3'-36-FAM-Modified

<400>  20
attacagagt cactgccacg gagtgcatcg cgcgggcc                               38


<210>  21
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  modified_base
<222>  (10)..(10)
<223>  2'-o-methyl nucleotide


<220>
<223>  3'-36-FAM-Modified

<400>  21
attacagagu cactgccacg gagtgcatcg cgcgggcc                               38


<210>  22
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  modified_base
<222>  (11)..(11)
<223>  2'-o-methyl nucleotide

<220>
<223>  3'-36-FAM-Modified

<400>  22
attacagagt cactgccacg gagtgcatcg cgcgggcc                               38


<210>  23
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  modified_base
<222>  (7)..(7)
<223>  2'-o-methyl nucleotide

<220>
<221>  modified_base
<222>  (12)..(12)
<223>  2'-o-methyl nucleotide


<220>
<223>  3'-36-FAM-Modified

<400>  23
attacagagt cactgccacg gagtgcatcg cgcgggcc                               38


<210>  24
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  modified_base
<222>  (7)..(7)
<223>  2'-o-methyl nucleotide

<220>
<221>  modified_base
<222>  (13)..(13)
<223>  2'-o-methyl nucleotide


<220>
<223>  3'-36-FAM-Modified

<400>  24
attacagagt cactgccacg gagtgcatcg cgcgggcc                               38


<210>  25
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<223>  5'-BHQ-1-Modified

<220>
<223>  3'-C3 Spacer-Modified

<400>  25
ggcccgcgcg atgcactccg tggcagtgac tctgtaat                               38


<210>  26
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic
       oligonucleotide


<220>
<221>  misc_feature
<222>  (6)..(12)
<223>  n is a, c, g, or t, unknown or other

<400>  26
gagtcnnnnn nn                                                           12


<210>  27
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic
       oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(7)
<223>  n is a, c, g, or t, unknown or other


<400>  27
nnnnnnngac tc                                                           12


<210>  28
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic
       oligonucleotide


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is a, c, g, or t, unknown or other

<220>
<221>  misc_feature
<222>  (7)..(12)
<223>  n is a, c, g, or t, unknown or other


<400>  28
gagtncnnnn nn                                                           12


<210>  29
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic
       oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(7)
<223>  n is a, c, g, or t, unknown or other


<400>  29
nnnnnnngac tc                                                           12


<210>  30
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic
       oligonucleotide


<220>
<221>  misc_feature
<222>  (6)..(10)
<223>  n is a, c, g, or t, unknown or other


<400>  30
ggatcnnnnn                                                              10


<210>  31
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic
       oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(5)
<223>  n is a, c, g, or t, unknown or other


<400>  31
nnnnngatcc                                                              10


<210>  32
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic
       oligonucleotide


<220>
<221>  misc_feature
<222>  (6)..(10)
<223>  n is a, c, g, or t, unknown or other


<400>  32
gagtcnnnnn                                                              10


<210>  33
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic
       oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(5)
<223>  n is a, c, g, or t, unknown or other



<400>  33
nnnnngactc                                                              10


<210>  34
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  enzyme recognition sequence


<220>
<221>  misc_feature
<222>  (6)..(10)
<223>  n is a, c, g, or t, unknown or other


<400>  34
gagtcnnnnn                                                              10


<210>  35
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic
       oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(5)
<223>  n is a, c, g, or t, unknown or other


<400>  35
nnnnngactc                                                              10


<210>  36
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic
       oligonucleotide


<220>
<221>  misc_feature
<222>  (6)..(14)
<223>  n is a, c, g, or t, unknown or other


<400>  36
gagtcnnnnn nnnn                                                         14


<210>  37
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic
       oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(9)
<223>  n is a, c, g, or t, unknown or other


<400>  37
nnnnnnnnng actc                                                         14


<210>  38
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  n is a, c, g, or t, unknown or other

<220>
<221>  misc_feature
<222>  (10)..(15)
<223>  n is a, c, g, or t, unknown or other

<220>
<221>  misc_feature
<222>  (21)..(24)
<223>  n is a, c, g, or t, unknown or other

<400>  38
nnnngactcn nnnnngagtc nnnn                                              24


<210>  39
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  n is a, c, g, or t, unknown or other

<220>
<221>  misc_feature
<222>  (10)..(13)
<223>  n is a, c, g, or t, unknown or other

<220>
<221>  misc_feature
<222>  (19)..(22)
<223>  n is a, c, g, or t, unknown or other

<400>  39
nnnngactcn nnngagtcnn nn                                                22


<210>  40
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer


<220>
<221>  modified_base
<222>  (25)..(25)
<223>  2'-o-methyl nucleotide

<220>
<221>  modified_base
<222>  (33)..(33)
<223>  2'-o-methyl nucleotide

<220>
<221>  modified_base
<222>  (34)..(34)
<223>  2'-o-methyl nucleotide

<220>
<221>  modified_base
<222>  (35)..(35)
<223>  2'-o-methyl nucleotide

<220>
<221>  modified_base
<222>  (36)..(36)
<223>  2'-o-methyl nucleotide

<220>
<221>  modified_base
<222>  (37)..(37)
<223>  2'-o-methyl nucleotide

<400>  40
tgactccata tggagtcaca tcaccgaaat accgcca                                37


<210>  41
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer


<220>
<221>  modified_base
<222>  (24)..(24)
<223>  2'-o-methyl nucleotide

<220>
<221>  modified_base
<222>  (32)..(32)
<223>  2'-o-methyl nucleotide

<220>
<221>  modified_base
<222>  (33)..(33)
<223>  2'-o-methyl nucleotide

<220>
<221>  modified_base
<222>  (34)..(34)
<223>  2'-o-methyl nucleotide

<220>
<221>  modified_base
<222>  (35)..(35)
<223>  2'-o-methyl nucleotide

<220>
<221>  modified_base
<222>  (36)..(36)
<223>  2'-o-methyl nucleotide

<400>  41
gactcgatat cgagtctttc caccgaaata ccgcca                                 36


<210>  42
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer


<220>
<221>  modified_base
<222>  (24)..(24)
<223>  2'-o-methyl nucleotide

<220>
<221>  modified_base
<222>  (32)..(32)
<223>  2'-o-methyl nucleotide

<220>
<221>  modified_base
<222>  (33)..(33)
<223>  2'-o-methyl nucleotide

<220>
<221>  modified_base
<222>  (34)..(34)
<223>  2'-o-methyl nucleotide

<220>
<221>  modified_base
<222>  (35)..(35)
<223>  2'-o-methyl nucleotide

<220>
<221>  modified_base
<222>  (36)..(36)
<223>  2'-o-methyl nucleotide

<400>  42
gaaagactcg cgagtctttc caccgaaata ccgcca                                 36


<210>  43
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer


<220>
<221>  modified_base
<222>  (25)..(25)
<223>  2'-o-methyl nucleotide

<220>
<221>  modified_base
<222>  (42)..(42)
<223>  2'-o-methyl nucleotide

<220>
<221>  modified_base
<222>  (43)..(43)
<223>  2'-o-methyl nucleotide

<220>
<221>  modified_base
<222>  (44)..(44)
<223>  2'-o-methyl nucleotide

<220>
<221>  modified_base
<222>  (45)..(45)
<223>  2'-o-methyl nucleotide

<220>
<221>  modified_base
<222>  (46)..(46)
<223>  2'-o-methyl nucleotide

<400>  43
tgactccata tggagtcaca tcggcatcat tattatcttt gugaac                      46


<210>  44
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer


<220>
<221>  modified_base
<222>  (24)..(24)
<223>  2'-o-methyl nucleotide

<220>
<221>  modified_base
<222>  (41)..(41)
<223>  2'-o-methyl nucleotide

<220>
<221>  modified_base
<222>  (42)..(42)
<223>  2'-o-methyl nucleotide

<220>
<221>  modified_base
<222>  (43)..(43)
<223>  2'-o-methyl nucleotide

<220>
<221>  modified_base
<222>  (44)..(44)
<223>  2'-o-methyl nucleotide

<220>
<221>  modified_base
<222>  (45)..(45)
<223>  2'-o-methyl nucleotide

<400>  44
gactcgatat cgagtctttc cggcatcatt attatctttg ugaac                       45


<210>  45
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer


<220>
<221>  modified_base
<222>  (24)..(24)
<223>  2'-o-methyl nucleotide

<220>
<221>  modified_base
<222>  (41)..(41)
<223>  2'-o-methyl nucleotide

<220>
<221>  modified_base
<222>  (42)..(42)
<223>  2'-o-methyl nucleotide

<220>
<221>  modified_base
<222>  (43)..(43)
<223>  2'-o-methyl nucleotide

<220>
<221>  modified_base
<222>  (44)..(44)
<223>  2'-o-methyl nucleotide

<220>
<221>  modified_base
<222>  (45)..(45)
<223>  2'-o-methyl nucleotide

<400>  45
gaaagactcg cgagtctttc cggcatcatt attatctttg ugaac                       45


<210>  46
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<223>  5'-CalRed-Modified

<220>
<223>  3'-BHQ2-Modified

<400>  46
cgcctgtgaa ctttattggc g                                                 21


