                         SEQUENCE LISTING

<110>  Wiesmann, et al.
 
<120>  TREATMENT OF BREAST CANCER

<130>  PAT056690 (27527/50292)

<150>  62/109,893
<151>  2015-01-30

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  1404
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  H-RX1 heavy chain coding region

<400>  1
atgggttggt cttgcatcat tctctttctc gtcgctaccg caactggtgt acactcccaa       60

gttcaacttc aagaatcagg ccccggactc gttaaaccct ctcaaactct ctctcttact      120

tgcactgtat ccgattactc tattacttca gactacgctt ggaactggat cagacaattt      180

cccggaaaag gactcgaatg gatgggatat atctcttact ctggctcaac ctcttacaac      240

ccctctctca aatctcgaat aacaatctca cgcgatactt ctaaaaatca attctcactt      300

caacttaact ccgttactgc cgccgacact gccgtttact actgtgcttc cttcgattac      360

gcccacgcta tggattattg gggacaagga actaccgtca ctgtcagctc agccagcaca      420

aagggcccat cggtcttccc cctggcaccc tcctccaaga gcacctctgg gggcacagcg      480

gccctgggct gcctggtcaa ggactacttc cccgaaccgg tgacggtgtc gtggaactca      540

ggcgccctga ccagcggcgt gcacaccttc ccggctgtcc tacagtcctc aggactctac      600

tccctcagca gcgtggtgac cgtgccctcc agcagcttgg gcacccagac ctacatctgc      660

aacgtgaatc acaagcccag caacaccaag gtggacaaga gagttgagcc caaatcttgt      720

gacaaaactc acacatgtcc accgtgccca gcacctgaac tcctgggggg accgtcagtc      780

ttcctcttcc ccccaaaacc caaggacacc ctcatgatct cccggacccc tgaggtcaca      840

tgcgtggtgg tggacgtgag ccacgaagac cctgaggtca agttcaactg gtacgtggac      900

ggcgtggagg tgcataatgc caagacaaag ccgcgggagg agcagtacaa cagcacgtac      960

cgtgtggtca gcgtcctcac cgtcctgcac caggactggc tgaatggcaa ggagtacaag     1020

tgcaaggtct ccaacaaagc cctcccagcc cccatcgaga aaaccatctc caaagccaaa     1080

gggcagcccc gagaaccaca ggtgtacacc ctgcccccat cccgggagga gatgaccaag     1140

aaccaggtca gcctgacctg cctggtcaaa ggcttctatc ccagcgacat cgccgtggag     1200

tgggagagca atgggcagcc ggagaacaac tacaagacca cgcctcccgt gctggactcc     1260

gacggctcct tcttcctcta tagcaagctc accgtggaca agagcaggtg gcagcagggg     1320

aacgtcttct catgctccgt gatgcatgag gctctgcaca accactacac gcagaagagc     1380

ctctccctgt ccccgggtaa atga                                            1404


<210>  2
<211>  467
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  H-RX1 heavy chain

<400>  2

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys 
            20                  25                  30          


Pro Ser Gln Thr Leu Ser Leu Thr Cys Thr Val Ser Asp Tyr Ser Ile 
        35                  40                  45              


Thr Ser Asp Tyr Ala Trp Asn Trp Ile Arg Gln Phe Pro Gly Lys Gly 
    50                  55                  60                  


Leu Glu Trp Met Gly Tyr Ile Ser Tyr Ser Gly Ser Thr Ser Tyr Asn 
65                  70                  75                  80  


Pro Ser Leu Lys Ser Arg Ile Thr Ile Ser Arg Asp Thr Ser Lys Asn 
                85                  90                  95      


Gln Phe Ser Leu Gln Leu Asn Ser Val Thr Ala Ala Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Ser Phe Asp Tyr Ala His Ala Met Asp Tyr Trp Gly 
        115                 120                 125             


Gln Gly Thr Thr Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser 
    130                 135                 140                 


Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala 
145                 150                 155                 160 


Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 
                165                 170                 175     


Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 
            180                 185                 190         


Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 
        195                 200                 205             


Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His 
    210                 215                 220                 


Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys 
225                 230                 235                 240 


Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
                245                 250                 255     


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
            260                 265                 270         


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
        275                 280                 285             


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
    290                 295                 300                 


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
305                 310                 315                 320 


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
                325                 330                 335     


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
            340                 345                 350         


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
        355                 360                 365             


Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 
    370                 375                 380                 


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
385                 390                 395                 400 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
                405                 410                 415     


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
            420                 425                 430         


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
        435                 440                 445             


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
    450                 455                 460                 


Pro Gly Lys 
465         


<210>  3
<211>  705
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  H-RX1 light chain coding region

<400>  3
atggtctcta ctccccaatt cctcgtcttt ctgctctttt ggatccctgc ttctcgcggc       60

gacatagttc tcacacaatc accagcattc ctctcagtta cacccggcga aaaagtaacc      120

tttacctgtc aggcttctca atctatcggc acttctattc actggtatca acaaaaaacc      180

gatcaagctc ctaaactcct cataaaatac gcatccgaat ccatctccgg tatcccctcc      240

agattttcag gctccggctc cggcacagat ttcaccctta ccattagctc agttgaagcc      300

gaagacgcag ctgattacta ctgtcaacaa ataaactcat ggcccactac tttcggcggc      360

ggcactaaac tcgaaataaa acgtacggtg gctgcaccat ctgtcttcat cttcccgcca      420

tctgatgagc agttgaaatc tggaactgcc tctgttgtgt gcctgctgaa taacttctat      480

cccagagagg ccaaagtaca gtggaaggtg gataacgccc tccaatcggg taactcccag      540

gagagtgtca cagagcagga cagcaaggac agcacctaca gcctcagcag caccctgacg      600

ctgagcaaag cagactacga gaaacacaaa gtctacgcct gcgaagtcac ccatcagggc      660

ctgagctcgc ccgtcacaaa gagcttcaac aggggagagt gttag                      705


<210>  4
<211>  234
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  H-RX1 light chain

<400>  4

Met Val Ser Thr Pro Gln Phe Leu Val Phe Leu Leu Phe Trp Ile Pro 
1               5                   10                  15      


Ala Ser Arg Gly Asp Ile Val Leu Thr Gln Ser Pro Ala Phe Leu Ser 
            20                  25                  30          


Val Thr Pro Gly Glu Lys Val Thr Phe Thr Cys Gln Ala Ser Gln Ser 
        35                  40                  45              


Ile Gly Thr Ser Ile His Trp Tyr Gln Gln Lys Thr Asp Gln Ala Pro 
    50                  55                  60                  


Lys Leu Leu Ile Lys Tyr Ala Ser Glu Ser Ile Ser Gly Ile Pro Ser 
65                  70                  75                  80  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
                85                  90                  95      


Ser Val Glu Ala Glu Asp Ala Ala Asp Tyr Tyr Cys Gln Gln Ile Asn 
            100                 105                 110         


Ser Trp Pro Thr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
        115                 120                 125             


Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln 
    130                 135                 140                 


Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr 
145                 150                 155                 160 


Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser 
                165                 170                 175     


Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr 
            180                 185                 190         


Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys 
        195                 200                 205             


His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro 
    210                 215                 220                 


Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
225                 230                 


<210>  5
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  H-CDR1

<400>  5

Ser Asp Tyr Ala Trp Asn 
1               5       


<210>  6
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  H-CDR2

<400>  6

Tyr Ile Ser Tyr Ser Gly Ser Thr Ser Tyr Asn Pro Ser Leu Lys Ser 
1               5                   10                  15      


<210>  7
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  H-CDR3

<400>  7

Phe Asp Tyr Ala His Ala Met Asp Tyr 
1               5                   


<210>  8
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  L-CDR1

<400>  8

Gln Ala Ser Gln Ser Ile Gly Thr Ser Ile His 
1               5                   10      


<210>  9
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  L-CDR2

<400>  9

Tyr Ala Ser Glu Ser Ile Ser 
1               5           


<210>  10
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  L-CDR3

<400>  10

Gln Gln Ile Asn Ser Trp Pro Thr Thr 
1               5                   


<210>  11
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  11

Arg Phe Arg Asp Asn Thr Pro Asn 
1               5               


<210>  12
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  12

Arg Phe Arg Asp Asn Thr Ala Asn 
1               5               


<210>  13
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  13

Ile Thr Phe Glu Phe Val Asp Gln Glu 
1               5                   


<210>  14
<211>  256
<212>  PRT
<213>  Homo sapiens

<400>  14

Met Thr Ala Pro Gly Ala Ala Gly Arg Cys Pro Pro Thr Thr Trp Leu 
1               5                   10                  15      


Gly Ser Leu Leu Leu Leu Val Cys Leu Leu Ala Ser Arg Ser Ile Thr 
            20                  25                  30          


Glu Glu Val Ser Glu Tyr Cys Ser His Met Ile Gly Ser Gly His Leu 
        35                  40                  45              


Gln Ser Leu Gln Arg Leu Ile Asp Ser Gln Met Glu Thr Ser Cys Gln 
    50                  55                  60                  


Ile Thr Phe Glu Phe Val Asp Gln Glu Gln Leu Lys Asp Pro Val Cys 
65                  70                  75                  80  


Tyr Leu Lys Lys Ala Phe Leu Leu Val Gln Asp Ile Met Glu Asp Thr 
                85                  90                  95      


Met Arg Phe Arg Asp Asn Thr Pro Asn Ala Ile Ala Ile Val Gln Leu 
            100                 105                 110         


Gln Glu Leu Ser Leu Arg Leu Lys Ser Cys Phe Thr Lys Asp Tyr Glu 
        115                 120                 125             


Glu His Asp Lys Ala Cys Val Arg Thr Phe Tyr Glu Thr Pro Leu Gln 
    130                 135                 140                 


Leu Leu Glu Lys Val Lys Asn Val Phe Asn Glu Thr Lys Asn Leu Leu 
145                 150                 155                 160 


Asp Lys Asp Trp Asn Ile Phe Ser Lys Asn Cys Asn Asn Ser Phe Ala 
                165                 170                 175     


Glu Cys Ser Ser Gln Gly His Glu Arg Gln Ser Glu Gly Ser Ser Ser 
            180                 185                 190         


Pro Gln Leu Gln Glu Ser Val Phe His Leu Leu Val Pro Ser Val Ile 
        195                 200                 205             


Leu Val Leu Leu Ala Val Gly Gly Leu Leu Phe Tyr Arg Trp Arg Arg 
    210                 215                 220                 


Arg Ser His Gln Glu Pro Gln Arg Ala Asp Ser Pro Leu Glu Gln Pro 
225                 230                 235                 240 


Glu Gly Ser Pro Leu Thr Gln Asp Asp Arg Gln Val Glu Leu Pro Val 
                245                 250                 255     


<210>  15
<211>  554
<212>  PRT
<213>  Homo sapiens

<400>  15

Met Thr Ala Pro Gly Ala Ala Gly Arg Cys Pro Pro Thr Thr Trp Leu 
1               5                   10                  15      


Gly Ser Leu Leu Leu Leu Val Cys Leu Leu Ala Ser Arg Ser Ile Thr 
            20                  25                  30          


Glu Glu Val Ser Glu Tyr Cys Ser His Met Ile Gly Ser Gly His Leu 
        35                  40                  45              


Gln Ser Leu Gln Arg Leu Ile Asp Ser Gln Met Glu Thr Ser Cys Gln 
    50                  55                  60                  


Ile Thr Phe Glu Phe Val Asp Gln Glu Gln Leu Lys Asp Pro Val Cys 
65                  70                  75                  80  


Tyr Leu Lys Lys Ala Phe Leu Leu Val Gln Asp Ile Met Glu Asp Thr 
                85                  90                  95      


Met Arg Phe Arg Asp Asn Thr Pro Asn Ala Ile Ala Ile Val Gln Leu 
            100                 105                 110         


Gln Glu Leu Ser Leu Arg Leu Lys Ser Cys Phe Thr Lys Asp Tyr Glu 
        115                 120                 125             


Glu His Asp Lys Ala Cys Val Arg Thr Phe Tyr Glu Thr Pro Leu Gln 
    130                 135                 140                 


Leu Leu Glu Lys Val Lys Asn Val Phe Asn Glu Thr Lys Asn Leu Leu 
145                 150                 155                 160 


Asp Lys Asp Trp Asn Ile Phe Ser Lys Asn Cys Asn Asn Ser Phe Ala 
                165                 170                 175     


Glu Cys Ser Ser Gln Asp Val Val Thr Lys Pro Asp Cys Asn Cys Leu 
            180                 185                 190         


Tyr Pro Lys Ala Ile Pro Ser Ser Asp Pro Ala Ser Val Ser Pro His 
        195                 200                 205             


Gln Pro Leu Ala Pro Ser Met Ala Pro Val Ala Gly Leu Thr Trp Glu 
    210                 215                 220                 


Asp Ser Glu Gly Thr Glu Gly Ser Ser Leu Leu Pro Gly Glu Gln Pro 
225                 230                 235                 240 


Leu His Thr Val Asp Pro Gly Ser Ala Lys Gln Arg Pro Pro Arg Ser 
                245                 250                 255     


Thr Cys Gln Ser Phe Glu Pro Pro Glu Thr Pro Val Val Lys Asp Ser 
            260                 265                 270         


Thr Ile Gly Gly Ser Pro Gln Pro Arg Pro Ser Val Gly Ala Phe Asn 
        275                 280                 285             


Pro Gly Met Glu Asp Ile Leu Asp Ser Ala Met Gly Thr Asn Trp Val 
    290                 295                 300                 


Pro Glu Glu Ala Ser Gly Glu Ala Ser Glu Ile Pro Val Pro Gln Gly 
305                 310                 315                 320 


Thr Glu Leu Ser Pro Ser Arg Pro Gly Gly Gly Ser Met Gln Thr Glu 
                325                 330                 335     


Pro Ala Arg Pro Ser Asn Phe Leu Ser Ala Ser Ser Pro Leu Pro Ala 
            340                 345                 350         


Ser Ala Lys Gly Gln Gln Pro Ala Asp Val Thr Gly Thr Ala Leu Pro 
        355                 360                 365             


Arg Val Gly Pro Val Arg Pro Thr Gly Gln Asp Trp Asn His Thr Pro 
    370                 375                 380                 


Gln Lys Thr Asp His Pro Ser Ala Leu Leu Arg Asp Pro Pro Glu Pro 
385                 390                 395                 400 


Gly Ser Pro Arg Ile Ser Ser Leu Arg Pro Gln Gly Leu Ser Asn Pro 
                405                 410                 415     


Ser Thr Leu Ser Ala Gln Pro Gln Leu Ser Arg Ser His Ser Ser Gly 
            420                 425                 430         


Ser Val Leu Pro Leu Gly Glu Leu Glu Gly Arg Arg Ser Thr Arg Asp 
        435                 440                 445             


Arg Arg Ser Pro Ala Glu Pro Glu Gly Gly Pro Ala Ser Glu Gly Ala 
    450                 455                 460                 


Ala Arg Pro Leu Pro Arg Phe Asn Ser Val Pro Leu Thr Asp Thr Gly 
465                 470                 475                 480 


His Glu Arg Gln Ser Glu Gly Ser Ser Ser Pro Gln Leu Gln Glu Ser 
                485                 490                 495     


Val Phe His Leu Leu Val Pro Ser Val Ile Leu Val Leu Leu Ala Val 
            500                 505                 510         


Gly Gly Leu Leu Phe Tyr Arg Trp Arg Arg Arg Ser His Gln Glu Pro 
        515                 520                 525             


Gln Arg Ala Asp Ser Pro Leu Glu Gln Pro Glu Gly Ser Pro Leu Thr 
    530                 535                 540                 


Gln Asp Asp Arg Gln Val Glu Leu Pro Val 
545                 550                 


<210>  16
<211>  438
<212>  PRT
<213>  Homo sapiens

<400>  16

Met Thr Ala Pro Gly Ala Ala Gly Arg Cys Pro Pro Thr Thr Trp Leu 
1               5                   10                  15      


Gly Ser Leu Leu Leu Leu Val Cys Leu Leu Ala Ser Arg Ser Ile Thr 
            20                  25                  30          


Glu Glu Val Ser Glu Tyr Cys Ser His Met Ile Gly Ser Gly His Leu 
        35                  40                  45              


Gln Ser Leu Gln Arg Leu Ile Asp Ser Gln Met Glu Thr Ser Cys Gln 
    50                  55                  60                  


Ile Thr Phe Glu Phe Val Asp Gln Glu Gln Leu Lys Asp Pro Val Cys 
65                  70                  75                  80  


Tyr Leu Lys Lys Ala Phe Leu Leu Val Gln Asp Ile Met Glu Asp Thr 
                85                  90                  95      


Met Arg Phe Arg Asp Asn Thr Pro Asn Ala Ile Ala Ile Val Gln Leu 
            100                 105                 110         


Gln Glu Leu Ser Leu Arg Leu Lys Ser Cys Phe Thr Lys Asp Tyr Glu 
        115                 120                 125             


Glu His Asp Lys Ala Cys Val Arg Thr Phe Tyr Glu Thr Pro Leu Gln 
    130                 135                 140                 


Leu Leu Glu Lys Val Lys Asn Val Phe Asn Glu Thr Lys Asn Leu Leu 
145                 150                 155                 160 


Asp Lys Asp Trp Asn Ile Phe Ser Lys Asn Cys Asn Asn Ser Phe Ala 
                165                 170                 175     


Glu Cys Ser Ser Gln Asp Val Val Thr Lys Pro Asp Cys Asn Cys Leu 
            180                 185                 190         


Tyr Pro Lys Ala Ile Pro Ser Ser Asp Pro Ala Ser Val Ser Pro His 
        195                 200                 205             


Gln Pro Leu Ala Pro Ser Met Ala Pro Val Ala Gly Leu Thr Trp Glu 
    210                 215                 220                 


Asp Ser Glu Gly Thr Glu Gly Ser Ser Leu Leu Pro Gly Glu Gln Pro 
225                 230                 235                 240 


Leu His Thr Val Asp Pro Gly Ser Ala Lys Gln Arg Pro Pro Arg Ser 
                245                 250                 255     


Thr Cys Gln Ser Phe Glu Pro Pro Glu Thr Pro Val Val Lys Asp Ser 
            260                 265                 270         


Thr Ile Gly Gly Ser Pro Gln Pro Arg Pro Ser Val Gly Ala Phe Asn 
        275                 280                 285             


Pro Gly Met Glu Asp Ile Leu Asp Ser Ala Met Gly Thr Asn Trp Val 
    290                 295                 300                 


Pro Glu Glu Ala Ser Gly Glu Ala Ser Glu Ile Pro Val Pro Gln Gly 
305                 310                 315                 320 


Thr Glu Leu Ser Pro Ser Arg Pro Gly Gly Gly Ser Met Gln Thr Glu 
                325                 330                 335     


Pro Ala Arg Pro Ser Asn Phe Leu Ser Ala Ser Ser Pro Leu Pro Ala 
            340                 345                 350         


Ser Ala Lys Gly Gln Gln Pro Ala Asp Val Thr Gly His Glu Arg Gln 
        355                 360                 365             


Ser Glu Gly Ser Ser Ser Pro Gln Leu Gln Glu Ser Val Phe His Leu 
    370                 375                 380                 


Leu Val Pro Ser Val Ile Leu Val Leu Leu Ala Val Gly Gly Leu Leu 
385                 390                 395                 400 


Phe Tyr Arg Trp Arg Arg Arg Ser His Gln Glu Pro Gln Arg Ala Asp 
                405                 410                 415     


Ser Pro Leu Glu Gln Pro Glu Gly Ser Pro Leu Thr Gln Asp Asp Arg 
            420                 425                 430         


Gln Val Glu Leu Pro Val 
        435             


<210>  17
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG4 hinge sequence

<400>  17

Cys Pro Ser Cys 
1               


<210>  18
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG1 hinge sequence

<400>  18

Cys Pro Pro Cys 
1               


