                         SEQUENCE LISTING

<110>  MEIOGENIX
       UNIVERSITE PIERRE ET MARIE CURIE (PARIS 6)
       INSTITUT CURIE
       CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
 
<120>  Procd pour induire des recombinaisons miotiques cibles

<130>  B1960PC

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  12349
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmide P1 comprenant un acide nuclique codant pour la protine
       de fusion Cas9 sauvage-Spo11


<220>
<221>  promoter
<222>  (1)..(397)
<223>  promoteur ADH1

<220>
<221>  gene
<222>  (431)..(5974)
<223>  Gne codant la protine de fusion Cas9-Spo11

<220>
<221>  terminator
<222>  (6010)..(6198)
<223>  terminateur ADH1

<220>
<221>  rep_origin
<222>  (6605)..(7287)
<223>  Origine de rplication bactrienne ColE1

<220>
<221>  gene
<222>  (7385)..(8045)
<223>  Gne de rsistance  l'ampiciline

<220>
<221>  gene
<222>  (8809)..(9631)
<223>  Gne TRP1

<220>
<221>  rep_origin
<222>  (9688)..(10126)
<223>  Origine de rplication F1

<220>
<221>  gene
<222>  (10344)..(11363)
<223>  Gne de rsistance au G418 (KanMx)

<400>  1
caacttcttt tctttttttt tcttttctct ctcccccgtt gttgtctcac catatccgca       60

atgacaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga cagcaccaac      120

agatgtcgtt gttccagagc tgatgagggg tatctcgaag cacacgaaac tttttccttc      180

cttcattcac gcacactact ctctaatgag caacggtata cggccttcct tccagttact      240

tgaatttgaa ataaaaaaaa gtttgctgtc ttgctatcaa gtataaatag acctgcaatt      300

attaatcttt tgtttcctcg tcattgttct cgttcccttt cttccttgtt tctttttctg      360

cacaatattt caagctatac caagcataca atcaactcca agcttgaagc aagcctcctg      420

aaagactagt atgggaaaac ctattcctaa tcctctgctg ggcctggatt ctaccggagg      480

catggcccct aagaaaaagc ggaaggtgga cggcggaggt attcatggag ttcctgctgc      540

gatggacaag aagtattcta tcggactgga tatcgggact aatagcgtcg ggtgggccgt      600

gatcactgac gagtacaagg tgccctctaa gaagttcaag gtgctcggga acaccgaccg      660

gcattccatc aagaaaaatc tgatcggagc tctcctcttt gattcagggg agaccgctga      720

agcaacccgc ctcaagcgga ctgctagacg gcggtacacc aggaggaaga accggatttg      780

ttaccttcaa gagatattct ccaacgaaat ggcaaaggtc gacgacagct tcttccatag      840

gctggaagaa tcattcctcg tggaagagga taagaagcat gaacggcatc ccatcttcgg      900

taatatcgtc gacgaggtgg cctatcacga gaaataccca accatctacc atcttcgcaa      960

aaagctggtg gactcaaccg acaaggcaga cctccggctt atctacctgg ccctggccca     1020

catgatcaag ttcagaggcc acttcctgat cgagggcgac ctcaatcctg acaatagcga     1080

tgtggataaa ctgttcatcc agctggtgca gacttacaac cagctctttg aagagaaccc     1140

catcaatgca agcggagtcg atgccaaggc cattctgtca gcccggctgt caaagagccg     1200

cagacttgag aatcttatcg ctcagctgcc gggtgaaaag aaaaatggac tgttcgggaa     1260

cctgattgct ctttcacttg ggctgactcc caatttcaag tctaatttcg acctggcaga     1320

ggatgccaag ctgcaactgt ccaaggacac ctatgatgac gatctcgaca acctcctggc     1380

ccagatcggt gaccaatacg ccgacctttt ccttgctgct aagaatcttt ctgacgccat     1440

cctgctgtct gacattctcc gcgtgaacac tgaaatcacc aaggcccctc tttcagcttc     1500

aatgattaag cggtatgatg agcaccacca ggacctgacc ctgcttaagg cactcgtccg     1560

gcagcagctt ccggagaagt acaaggaaat cttctttgac cagtcaaaga atggatacgc     1620

cggctacatc gacggaggtg cctcccaaga ggaattttat aagtttatca aacctatcct     1680

tgagaagatg gacggcaccg aagagctcct cgtgaaactg aatcgggagg atctgctgcg     1740

gaagcagcgc actttcgaca atgggagcat tccccaccag atccatcttg gggagcttca     1800

cgccatcctt cggcgccaag aggacttcta cccctttctt aaggacaaca gggagaagat     1860

tgagaaaatt ctcactttcc gcatccccta ctacgtggga cccctcgcca gaggaaatag     1920

ccggtttgct tggatgacca gaaagtcaga agaaactatc actccctgga acttcgaaga     1980

ggtggtggac aagggagcca gcgctcagtc attcatcgaa cggatgacta acttcgataa     2040

gaacctcccc aatgagaagg tcctgccgaa acattccctg ctctacgagt actttaccgt     2100

gtacaacgag ctgaccaagg tgaaatatgt caccgaaggg atgaggaagc ccgcattcct     2160

gtcaggcgaa caaaagaagg caattgtgga ccttctgttc aagaccaata gaaaggtgac     2220

cgtgaagcag ctgaaggagg actatttcaa gaaaattgaa tgcttcgact ctgtggagat     2280

tagcggggtc gaagatcggt tcaacgcaag cctgggtacc taccatgatc tgcttaagat     2340

catcaaggac aaggattttc tggacaatga ggagaacgag gacatccttg aggacattgt     2400

cctgactctc actctgttcg aggaccggga aatgatcgag gagaggctta agacctacgc     2460

ccatctgttc gacgataaag tgatgaagca acttaaacgg agaagatata ccggatgggg     2520

acgccttagc cgcaaactca tcaacggaat ccgggacaaa cagagcggaa agaccattct     2580

tgatttcctt aagagcgacg gattcgctaa tcgcaacttc atgcaactta tccatgatga     2640

ttccctgacc tttaaggagg acatccagaa ggcccaagtg tctggacaag gtgactcact     2700

gcacgagcat atcgcaaatc tggctggttc acccgctatt aagaagggta ttctccagac     2760

cgtgaaagtc gtggacgagc tggtcaaggt gatgggtcgc cataaaccag agaacattgt     2820

catcgagatg gccagggaaa accagactac ccagaaggga cagaagaaca gcagggagcg     2880

gatgaaaaga attgaggaag ggattaagga gctcgggtca cagatcctta aagagcaccc     2940

ggtggaaaac acccagcttc agaatgagaa gctctatctg tactaccttc aaaatggacg     3000

cgatatgtat gtggaccaag agcttgatat caacaggctc tcagactacg acgtggacca     3060

tatcgtccct cagagcttcc tcaaagacga ctcaattgac aataaggtgc tgactcgctc     3120

agacaagaac cggggaaagt cagataacgt gccctcagag gaagtcgtga aaaagatgaa     3180

gaactattgg cgccagcttc tgaacgcaaa gctgatcact cagcggaagt tcgacaatct     3240

cactaaggct gagaggggcg gactgagcga actggacaaa gcaggattca ttaaacggca     3300

acttgtggag actcggcaga ttactaaaca tgtcgcccaa atccttgact cacgcatgaa     3360

taccaagtac gacgaaaacg acaaacttat ccgcgaggtg aaggtgatta ccctgaagtc     3420

caagctggtc agcgatttca gaaaggactt tcaattctac aaagtgcggg agatcaataa     3480

ctatcatcat gctcatgacg catatctgaa tgccgtggtg ggaaccgccc tgatcaagaa     3540

gtacccaaag ctggaaagcg agttcgtgta cggagactac aaggtctacg acgtgcgcaa     3600

gatgattgcc aaatctgagc aggagatcgg aaaggccacc gcaaagtact tcttctacag     3660

caacatcatg aatttcttca agaccgaaat cacccttgca aacggtgaga tccggaagag     3720

gccgctcatc gagactaatg gggagactgg cgaaatcgtg tgggacaagg gcagagattt     3780

cgctaccgtg cgcaaagtgc tttctatgcc tcaagtgaac atcgtgaaga aaaccgaggt     3840

gcaaaccgga ggcttttcta aggaatcaat cctccccaag cgcaactccg acaagctcat     3900

tgcaaggaag aaggattggg accctaagaa gtacggcgga ttcgattcac caactgtggc     3960

ttattctgtc ctggtcgtgg ctaaggtgga aaaaggaaag tctaagaagc tcaagagcgt     4020

gaaggaactg ctgggtatca ccattatgga gcgcagctcc ttcgagaaga acccaattga     4080

ctttctcgaa gccaaaggtt acaaggaagt caagaaggac cttatcatca agctcccaaa     4140

gtatagcctg ttcgaactgg agaatgggcg gaagcggatg ctcgcctccg ctggcgaact     4200

tcagaagggt aatgagctgg ctctcccctc caagtacgtg aatttcctct accttgcaag     4260

ccattacgag aagctgaagg ggagccccga ggacaacgag caaaagcaac tgtttgtgga     4320

gcagcataag cattatctgg acgagatcat tgagcagatt tccgagtttt ctaaacgcgt     4380

cattctcgct gatgccaacc tcgataaagt ccttagcgca tacaataagc acagagacaa     4440

accaattcgg gagcaggctg agaatatcat ccacctgttc accctcacca atcttggtgc     4500

ccctgccgca ttcaagtact tcgacaccac catcgaccgg aaacgctata cctccaccaa     4560

agaagtgctg gacgccaccc tcatccacca gagcatcacc ggactttacg aaactcggat     4620

tgacctctca cagctcggag gggatccgga atttatggcc atggaggccc cggggatccg     4680

tatggctttg gagggattgc ggaaaaaata taaaacaagg caggaattgg tcaaagcact     4740

cactcctaaa agacggtcca ttcacttgaa ctccaatggt cactccaacg gaactccctg     4800

ttcaaacgca gatgttttgg ctcatattaa gcatttcctg tcattggcgg ctaattcatt     4860

agagcaacat caacagccta tttcaatcgt ctttcaaaac aaaaaaaaaa aaggcgatac     4920

aagcagtcct gacattcaca caacattgga cttccctttg aatggcccgc atctatgcac     4980

tcatcagttc aagttgaaaa gatgcgcaat ccttttaaac ttattgaaag tcgttatgga     5040

aaaattaccg ctaggtaaaa acactacagt gagagatatc ttctactcca acgtggaatt     5100

gtttcaaaga caagcaaacg tagtccagtg gctggacgtt atacgcttta atttcaagct     5160

ctctccaaga aaatccttaa acattatacc agctcaaaag ggtttagttt attcgccttt     5220

ccccattgat atttatgaca atattctgac atgtgaaaat gaaccaaaga tgcaaaagca     5280

aacaattttc cctggtaagc cctgtctaat tccatttttc caagatgatg cggtcatcaa     5340

gttagggaca acaagtatgt gtaatattgt aatagtggaa aaagaagctg tcttcaccaa     5400

attagtaaat aattatcaca agttgagtac aaataccatg ctcattacag gtaagggatt     5460

tccagatttc ttgacaaggt tattcctaaa aaaactagaa caatattgct ccaaattgat     5520

atcggactgt tctatattta ccgatgcgga cccctatggg attagcatag ccctaaatta     5580

tactcactcg aatgaacgca acgcttatat ttgcacgatg gcaaactata aaggaattcg     5640

tattacgcaa gttttggcac aaaataatga agtgcataac aaatccattc aattattgag     5700

tttgaatcag cgcgactact ccttagccaa gaatttgata gcatctctga ctgccaacag     5760

ctgggatatt gcaacttcac cattaaagaa cgtcatcata gaatgtcagc gggaaatttt     5820

tttccaaaag aaagctgaaa tgaacgagat tgatgccaga atttttgaat acaaatccca     5880

ccaccatcat catcacggag actacaagga tgacgatgac aaggactaca aggatgacga     5940

tgacaaggac tacaaggatg acgatgacaa gtaatgagtc gacaacccct gcagccaagc     6000

taattccggg cgaatttctt atgatttatg atttttatta ttaaataagt tataaaaaaa     6060

ataagtgtat acaaatttta aagtgactct taggttttaa aacgaaaatt cttgttcttg     6120

agtaactctt tcctgtaggt caggttgctt tctcaggtat agcatgaggt cgctcttatt     6180

gaccacacct ctaccggcat gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt     6240

gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag     6300

cctggggtgc ctaatgagtg aggtaactca cattaattgc gttgcgctca ctgcccgctt     6360

tccagtcggg aaacctgtcg tgccagctgg attaatgaat cggccaacgc gcggggagag     6420

gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg     6480

ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat     6540

caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta     6600

aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa     6660

atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc     6720

cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt     6780

ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca     6840

gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg     6900

accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat     6960

cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta     7020

cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct     7080

gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac     7140

aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa     7200

aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa     7260

actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt     7320

taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca     7380

gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca     7440

tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc     7500

ccagtgctgc aatgataccg cgagacccac gctcaccggc ctccagattt atcagcaata     7560

aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc     7620

cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc     7680

aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca     7740

ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa     7800

gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca     7860

ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt     7920

tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt     7980

tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg     8040

ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga     8100

tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc     8160

agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg     8220

acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag     8280

ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     8340

gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg     8400

acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat     8460

gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg     8520

gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc     8580

tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccataa acgacattac     8640

tatatatata atataggaag catttaatag acagcatcgt aatatatgtg tactttgcag     8700

ttatgacgcc agatggcagt agtggaagat attctttatt gaaaaatagc ttgtcacctt     8760

acgtacaatc ttgatccgga gcttttcttt ttttgccgat taagaattaa ttcggtcgaa     8820

aaaagaaaag gagagggcca agagggaggg cattggtgac tattgagcac gtgagtatac     8880

gtgattaagc acacaaaggc agcttggagt atgtctgtta ttaatttcac aggtagttct     8940

ggtccattgg tgaaagtttg cggcttgcag agcacagagg ccgcagaatg tgctctagat     9000

tccgatgctg acttgctggg tattatatgt gtgcccaata gaaagagaac aattgacccg     9060

gttattgcaa ggaaaatttc aagtcttgta aaagcatata aaaatagttc aggcactccg     9120

aaatacttgg ttggcgtgtt tcgtaatcaa cctaaggagg atgttttggc tctggtcaat     9180

gattacggca ttgatatcgt ccaactgcat ggagatgagt cgtggcaaga ataccaagag     9240

ttcctcggtt tgccagttat taaaagactc gtatttccaa aagactgcaa catactactc     9300

agtgcagctt cacagaaacc tcattcgttt attcccttgt ttgattcaga agcaggtggg     9360

acaggtgaac ttttggattg gaactcgatt tctgactggg ttggaaggca agagagcccc     9420

gaaagcttac attttatgtt agctggtgga ctgacgccag aaaatgttgg tgatgcgctt     9480

agattaaatg gcgttattgg tgttgatgta agcggaggtg tggagacaaa tggtgtaaaa     9540

gactctaaca aaatagcaaa tttcgtcaaa aatgctaaga aataggttat tactgagtag     9600

tatttattta agtattgttt gtgcacttgc cgatctatgc ggtgtgaaat accgcacaga     9660

tgcgtaagga gaaaataccg catcaggaaa ttgtaaacgt taatattttg ttaaaattcg     9720

cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc     9780

cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga     9840

gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg     9900

atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag     9960

cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga    10020

acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg    10080

tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg    10140

cgtcgcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct    10200

cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa    10260

cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtcgtcca agctttcgat    10320

catcgatgaa ttcgagctcg ttttcgacag cagtatagcg accagcattc acatacgatt    10380

gacgcatgat attactttct gcgcacttaa cttcgcatct gggcagatga tgtcgaggcg    10440

aaaaaaaata taaatcacgc taacatttga ttaaaataga acaactacaa tataaaaaaa    10500

ctatacaaat gacaagttct tgaaaacaag aatcttttta ttgtcagtac tgattagaaa    10560

aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat    10620

ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg    10680

gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat    10740

ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc    10800

ggtgagaatg gcaaaagctt atgcatttct ttccagactt gttcaacagg ccagccatta    10860

cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga    10920

gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac    10980

cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct    11040

aatacctgga atgctgtttt gccggggatc gcagtggtga gtaaccatgc atcatcagga    11100

gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg    11160

accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct    11220

ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg    11280

cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg cggcctcgaa    11340

acgtgagtct tttccttacc catggttgtt tatgttcgga tgtgatgtga gaactgtatc    11400

ctagcaagat tttaaaagga agtatatgaa agaagaacct cagtggcaaa tcctaacctt    11460

ttatatttct ctacaggggc gcggcgtggg gacaattcaa cgcgtctgtg aggggagcgt    11520

ttccctgctc gcaggtctgc agcgaggagc cgtaattttt gcttcgcgcc gtgcggccat    11580

caaaatgtat ggatgcaaat gattatacat ggggatgtat gggctaaatg tacgggcgac    11640

agtcacatca tgcccctgag ctgcgcacgt caagactgtc aaggagggta ttctgggcct    11700

ccatgtcgct ggccgggtga cccggcgggg acgaggcaag ctaaacagat ctggcgcgcc    11760

ttaattaacc ccgagctcga gatcccgagc ttgcaaatta aagccttcga gcgtcccaaa    11820

accttctcaa gcaaggtttt cagtataatg ttacatgcgt acacgcgtct gtacagaaaa    11880

aaaagaaaaa tttgaaatat aaataacgtt cttaatacta acataactat aaaaaaataa    11940

atagggacct agacttcagg ttgtctaact ccttcctttt cggttagagc ggatgtgggg    12000

ggagggcgtg aatgtaagcg tgacataact aattacatga tatccttttg ttgtttccgg    12060

gtgtacaata tggacttcct cttttctggc aaccaaaccc atacatcggg attcctataa    12120

taccttcgtt ggtctcccta acatgtaggt ggcggagggg agatatacaa tagaacagat    12180

accagacaag acataatggg ctaaacaaaa ctacaccaat tacactgcct cattgatggt    12240

ggtacataac gaactaatac tgtagcccta gacttgatag ccatcatcat atcgaagttt    12300

cactaccctt tttccatttg ccatctattg aagtaataat aggcgcatg                12349


<210>  2
<211>  12349
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmide P2 comprenant un acide nuclique codant pour la protine
       de fusion Cas9*-Spo11


<220>
<221>  promoter
<222>  (1)..(397)
<223>  Promoteur ADH1

<220>
<221>  gene
<222>  (431)..(5974)
<223>  Gne codant la protine de fusion Ca9*-Spo11

<220>
<221>  terminator
<222>  (6010)..(6198)
<223>  Terminateur ADH1

<220>
<221>  rep_origin
<222>  (6605)..(7287)
<223>  Origine de rplication bactrienne ColE1

<220>
<221>  gene
<222>  (7385)..(8045)
<223>  Gne de rsistance  l'ampiciline

<220>
<221>  gene
<222>  (8809)..(9631)
<223>  Gne TRP1

<220>
<221>  rep_origin
<222>  (9688)..(10126)
<223>  Origine de rplication F1

<220>
<221>  gene
<222>  (10344)..(11363)
<223>  Gne de rsistance au G418 (KanMX)

<400>  2
caacttcttt tctttttttt tcttttctct ctcccccgtt gttgtctcac catatccgca       60

atgacaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga cagcaccaac      120

agatgtcgtt gttccagagc tgatgagggg tatctcgaag cacacgaaac tttttccttc      180

cttcattcac gcacactact ctctaatgag caacggtata cggccttcct tccagttact      240

tgaatttgaa ataaaaaaaa gtttgctgtc ttgctatcaa gtataaatag acctgcaatt      300

attaatcttt tgtttcctcg tcattgttct cgttcccttt cttccttgtt tctttttctg      360

cacaatattt caagctatac caagcataca atcaactcca agcttgaagc aagcctcctg      420

aaagactagt atgggaaaac ctattcctaa tcctctgctg ggcctggatt ctaccggagg      480

catggcccct aagaaaaagc ggaaggtgga cggcggaggt attcatggag ttcctgctgc      540

gatggacaag aagtattcta tcggactggc catcgggact aatagcgtcg ggtgggccgt      600

gatcactgac gagtacaagg tgccctctaa gaagttcaag gtgctcggga acaccgaccg      660

gcattccatc aagaaaaatc tgatcggagc tctcctcttt gattcagggg agaccgctga      720

agcaacccgc ctcaagcgga ctgctagacg gcggtacacc aggaggaaga accggatttg      780

ttaccttcaa gagatattct ccaacgaaat ggcaaaggtc gacgacagct tcttccatag      840

gctggaagaa tcattcctcg tggaagagga taagaagcat gaacggcatc ccatcttcgg      900

taatatcgtc gacgaggtgg cctatcacga gaaataccca accatctacc atcttcgcaa      960

aaagctggtg gactcaaccg acaaggcaga cctccggctt atctacctgg ccctggccca     1020

catgatcaag ttcagaggcc acttcctgat cgagggcgac ctcaatcctg acaatagcga     1080

tgtggataaa ctgttcatcc agctggtgca gacttacaac cagctctttg aagagaaccc     1140

catcaatgca agcggagtcg atgccaaggc cattctgtca gcccggctgt caaagagccg     1200

cagacttgag aatcttatcg ctcagctgcc gggtgaaaag aaaaatggac tgttcgggaa     1260

cctgattgct ctttcacttg ggctgactcc caatttcaag tctaatttcg acctggcaga     1320

ggatgccaag ctgcaactgt ccaaggacac ctatgatgac gatctcgaca acctcctggc     1380

ccagatcggt gaccaatacg ccgacctttt ccttgctgct aagaatcttt ctgacgccat     1440

cctgctgtct gacattctcc gcgtgaacac tgaaatcacc aaggcccctc tttcagcttc     1500

aatgattaag cggtatgatg agcaccacca ggacctgacc ctgcttaagg cactcgtccg     1560

gcagcagctt ccggagaagt acaaggaaat cttctttgac cagtcaaaga atggatacgc     1620

cggctacatc gacggaggtg cctcccaaga ggaattttat aagtttatca aacctatcct     1680

tgagaagatg gacggcaccg aagagctcct cgtgaaactg aatcgggagg atctgctgcg     1740

gaagcagcgc actttcgaca atgggagcat tccccaccag atccatcttg gggagcttca     1800

cgccatcctt cggcgccaag aggacttcta cccctttctt aaggacaaca gggagaagat     1860

tgagaaaatt ctcactttcc gcatccccta ctacgtggga cccctcgcca gaggaaatag     1920

ccggtttgct tggatgacca gaaagtcaga agaaactatc actccctgga acttcgaaga     1980

ggtggtggac aagggagcca gcgctcagtc attcatcgaa cggatgacta acttcgataa     2040

gaacctcccc aatgagaagg tcctgccgaa acattccctg ctctacgagt actttaccgt     2100

gtacaacgag ctgaccaagg tgaaatatgt caccgaaggg atgaggaagc ccgcattcct     2160

gtcaggcgaa caaaagaagg caattgtgga ccttctgttc aagaccaata gaaaggtgac     2220

cgtgaagcag ctgaaggagg actatttcaa gaaaattgaa tgcttcgact ctgtggagat     2280

tagcggggtc gaagatcggt tcaacgcaag cctgggtacc taccatgatc tgcttaagat     2340

catcaaggac aaggattttc tggacaatga ggagaacgag gacatccttg aggacattgt     2400

cctgactctc actctgttcg aggaccggga aatgatcgag gagaggctta agacctacgc     2460

ccatctgttc gacgataaag tgatgaagca acttaaacgg agaagatata ccggatgggg     2520

acgccttagc cgcaaactca tcaacggaat ccgggacaaa cagagcggaa agaccattct     2580

tgatttcctt aagagcgacg gattcgctaa tcgcaacttc atgcaactta tccatgatga     2640

ttccctgacc tttaaggagg acatccagaa ggcccaagtg tctggacaag gtgactcact     2700

gcacgagcat atcgcaaatc tggctggttc acccgctatt aagaagggta ttctccagac     2760

cgtgaaagtc gtggacgagc tggtcaaggt gatgggtcgc cataaaccag agaacattgt     2820

catcgagatg gccagggaaa accagactac ccagaaggga cagaagaaca gcagggagcg     2880

gatgaaaaga attgaggaag ggattaagga gctcgggtca cagatcctta aagagcaccc     2940

ggtggaaaac acccagcttc agaatgagaa gctctatctg tactaccttc aaaatggacg     3000

cgatatgtat gtggaccaag agcttgatat caacaggctc tcagactacg acgtggacgc     3060

tatcgtccct cagagcttcc tcaaagacga ctcaattgac aataaggtgc tgactcgctc     3120

agacaagaac cggggaaagt cagataacgt gccctcagag gaagtcgtga aaaagatgaa     3180

gaactattgg cgccagcttc tgaacgcaaa gctgatcact cagcggaagt tcgacaatct     3240

cactaaggct gagaggggcg gactgagcga actggacaaa gcaggattca ttaaacggca     3300

acttgtggag actcggcaga ttactaaaca tgtcgcccaa atccttgact cacgcatgaa     3360

taccaagtac gacgaaaacg acaaacttat ccgcgaggtg aaggtgatta ccctgaagtc     3420

caagctggtc agcgatttca gaaaggactt tcaattctac aaagtgcggg agatcaataa     3480

ctatcatcat gctcatgacg catatctgaa tgccgtggtg ggaaccgccc tgatcaagaa     3540

gtacccaaag ctggaaagcg agttcgtgta cggagactac aaggtctacg acgtgcgcaa     3600

gatgattgcc aaatctgagc aggagatcgg aaaggccacc gcaaagtact tcttctacag     3660

caacatcatg aatttcttca agaccgaaat cacccttgca aacggtgaga tccggaagag     3720

gccgctcatc gagactaatg gggagactgg cgaaatcgtg tgggacaagg gcagagattt     3780

cgctaccgtg cgcaaagtgc tttctatgcc tcaagtgaac atcgtgaaga aaaccgaggt     3840

gcaaaccgga ggcttttcta aggaatcaat cctccccaag cgcaactccg acaagctcat     3900

tgcaaggaag aaggattggg accctaagaa gtacggcgga ttcgattcac caactgtggc     3960

ttattctgtc ctggtcgtgg ctaaggtgga aaaaggaaag tctaagaagc tcaagagcgt     4020

gaaggaactg ctgggtatca ccattatgga gcgcagctcc ttcgagaaga acccaattga     4080

ctttctcgaa gccaaaggtt acaaggaagt caagaaggac cttatcatca agctcccaaa     4140

gtatagcctg ttcgaactgg agaatgggcg gaagcggatg ctcgcctccg ctggcgaact     4200

tcagaagggt aatgagctgg ctctcccctc caagtacgtg aatttcctct accttgcaag     4260

ccattacgag aagctgaagg ggagccccga ggacaacgag caaaagcaac tgtttgtgga     4320

gcagcataag cattatctgg acgagatcat tgagcagatt tccgagtttt ctaaacgcgt     4380

cattctcgct gatgccaacc tcgataaagt ccttagcgca tacaataagc acagagacaa     4440

accaattcgg gagcaggctg agaatatcat ccacctgttc accctcacca atcttggtgc     4500

ccctgccgca ttcaagtact tcgacaccac catcgaccgg aaacgctata cctccaccaa     4560

agaagtgctg gacgccaccc tcatccacca gagcatcacc ggactttacg aaactcggat     4620

tgacctctca cagctcggag gggatccgga atttatggcc atggaggccc cggggatccg     4680

tatggctttg gagggattgc ggaaaaaata taaaacaagg caggaattgg tcaaagcact     4740

cactcctaaa agacggtcca ttcacttgaa ctccaatggt cactccaacg gaactccctg     4800

ttcaaacgca gatgttttgg ctcatattaa gcatttcctg tcattggcgg ctaattcatt     4860

agagcaacat caacagccta tttcaatcgt ctttcaaaac aaaaaaaaaa aaggcgatac     4920

aagcagtcct gacattcaca caacattgga cttccctttg aatggcccgc atctatgcac     4980

tcatcagttc aagttgaaaa gatgcgcaat ccttttaaac ttattgaaag tcgttatgga     5040

aaaattaccg ctaggtaaaa acactacagt gagagatatc ttctactcca acgtggaatt     5100

gtttcaaaga caagcaaacg tagtccagtg gctggacgtt atacgcttta atttcaagct     5160

ctctccaaga aaatccttaa acattatacc agctcaaaag ggtttagttt attcgccttt     5220

ccccattgat atttatgaca atattctgac atgtgaaaat gaaccaaaga tgcaaaagca     5280

aacaattttc cctggtaagc cctgtctaat tccatttttc caagatgatg cggtcatcaa     5340

gttagggaca acaagtatgt gtaatattgt aatagtggaa aaagaagctg tcttcaccaa     5400

attagtaaat aattatcaca agttgagtac aaataccatg ctcattacag gtaagggatt     5460

tccagatttc ttgacaaggt tattcctaaa aaaactagaa caatattgct ccaaattgat     5520

atcggactgt tctatattta ccgatgcgga cccctatggg attagcatag ccctaaatta     5580

tactcactcg aatgaacgca acgcttatat ttgcacgatg gcaaactata aaggaattcg     5640

tattacgcaa gttttggcac aaaataatga agtgcataac aaatccattc aattattgag     5700

tttgaatcag cgcgactact ccttagccaa gaatttgata gcatctctga ctgccaacag     5760

ctgggatatt gcaacttcac cattaaagaa cgtcatcata gaatgtcagc gggaaatttt     5820

tttccaaaag aaagctgaaa tgaacgagat tgatgccaga atttttgaat acaaatccca     5880

ccaccatcat catcacggag actacaagga tgacgatgac aaggactaca aggatgacga     5940

tgacaaggac tacaaggatg acgatgacaa gtaatgagtc gacaacccct gcagccaagc     6000

taattccggg cgaatttctt atgatttatg atttttatta ttaaataagt tataaaaaaa     6060

ataagtgtat acaaatttta aagtgactct taggttttaa aacgaaaatt cttgttcttg     6120

agtaactctt tcctgtaggt caggttgctt tctcaggtat agcatgaggt cgctcttatt     6180

gaccacacct ctaccggcat gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt     6240

gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag     6300

cctggggtgc ctaatgagtg aggtaactca cattaattgc gttgcgctca ctgcccgctt     6360

tccagtcggg aaacctgtcg tgccagctgg attaatgaat cggccaacgc gcggggagag     6420

gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg     6480

ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat     6540

caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta     6600

aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa     6660

atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc     6720

cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt     6780

ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca     6840

gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg     6900

accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat     6960

cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta     7020

cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct     7080

gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac     7140

aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa     7200

aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa     7260

actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt     7320

taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca     7380

gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca     7440

tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc     7500

ccagtgctgc aatgataccg cgagacccac gctcaccggc ctccagattt atcagcaata     7560

aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc     7620

cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc     7680

aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca     7740

ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa     7800

gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca     7860

ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt     7920

tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt     7980

tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg     8040

ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga     8100

tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc     8160

agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg     8220

acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag     8280

ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     8340

gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg     8400

acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat     8460

gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg     8520

gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc     8580

tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccataa acgacattac     8640

tatatatata atataggaag catttaatag acagcatcgt aatatatgtg tactttgcag     8700

ttatgacgcc agatggcagt agtggaagat attctttatt gaaaaatagc ttgtcacctt     8760

acgtacaatc ttgatccgga gcttttcttt ttttgccgat taagaattaa ttcggtcgaa     8820

aaaagaaaag gagagggcca agagggaggg cattggtgac tattgagcac gtgagtatac     8880

gtgattaagc acacaaaggc agcttggagt atgtctgtta ttaatttcac aggtagttct     8940

ggtccattgg tgaaagtttg cggcttgcag agcacagagg ccgcagaatg tgctctagat     9000

tccgatgctg acttgctggg tattatatgt gtgcccaata gaaagagaac aattgacccg     9060

gttattgcaa ggaaaatttc aagtcttgta aaagcatata aaaatagttc aggcactccg     9120

aaatacttgg ttggcgtgtt tcgtaatcaa cctaaggagg atgttttggc tctggtcaat     9180

gattacggca ttgatatcgt ccaactgcat ggagatgagt cgtggcaaga ataccaagag     9240

ttcctcggtt tgccagttat taaaagactc gtatttccaa aagactgcaa catactactc     9300

agtgcagctt cacagaaacc tcattcgttt attcccttgt ttgattcaga agcaggtggg     9360

acaggtgaac ttttggattg gaactcgatt tctgactggg ttggaaggca agagagcccc     9420

gaaagcttac attttatgtt agctggtgga ctgacgccag aaaatgttgg tgatgcgctt     9480

agattaaatg gcgttattgg tgttgatgta agcggaggtg tggagacaaa tggtgtaaaa     9540

gactctaaca aaatagcaaa tttcgtcaaa aatgctaaga aataggttat tactgagtag     9600

tatttattta agtattgttt gtgcacttgc cgatctatgc ggtgtgaaat accgcacaga     9660

tgcgtaagga gaaaataccg catcaggaaa ttgtaaacgt taatattttg ttaaaattcg     9720

cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc     9780

cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga     9840

gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg     9900

atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag     9960

cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga    10020

acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg    10080

tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg    10140

cgtcgcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct    10200

cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa    10260

cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtcgtcca agctttcgat    10320

catcgatgaa ttcgagctcg ttttcgacag cagtatagcg accagcattc acatacgatt    10380

gacgcatgat attactttct gcgcacttaa cttcgcatct gggcagatga tgtcgaggcg    10440

aaaaaaaata taaatcacgc taacatttga ttaaaataga acaactacaa tataaaaaaa    10500

ctatacaaat gacaagttct tgaaaacaag aatcttttta ttgtcagtac tgattagaaa    10560

aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat    10620

ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg    10680

gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat    10740

ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc    10800

ggtgagaatg gcaaaagctt atgcatttct ttccagactt gttcaacagg ccagccatta    10860

cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga    10920

gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac    10980

cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct    11040

aatacctgga atgctgtttt gccggggatc gcagtggtga gtaaccatgc atcatcagga    11100

gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg    11160

accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct    11220

ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg    11280

cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg cggcctcgaa    11340

acgtgagtct tttccttacc catggttgtt tatgttcgga tgtgatgtga gaactgtatc    11400

ctagcaagat tttaaaagga agtatatgaa agaagaacct cagtggcaaa tcctaacctt    11460

ttatatttct ctacaggggc gcggcgtggg gacaattcaa cgcgtctgtg aggggagcgt    11520

ttccctgctc gcaggtctgc agcgaggagc cgtaattttt gcttcgcgcc gtgcggccat    11580

caaaatgtat ggatgcaaat gattatacat ggggatgtat gggctaaatg tacgggcgac    11640

agtcacatca tgcccctgag ctgcgcacgt caagactgtc aaggagggta ttctgggcct    11700

ccatgtcgct ggccgggtga cccggcgggg acgaggcaag ctaaacagat ctggcgcgcc    11760

ttaattaacc ccgagctcga gatcccgagc ttgcaaatta aagccttcga gcgtcccaaa    11820

accttctcaa gcaaggtttt cagtataatg ttacatgcgt acacgcgtct gtacagaaaa    11880

aaaagaaaaa tttgaaatat aaataacgtt cttaatacta acataactat aaaaaaataa    11940

atagggacct agacttcagg ttgtctaact ccttcctttt cggttagagc ggatgtgggg    12000

ggagggcgtg aatgtaagcg tgacataact aattacatga tatccttttg ttgtttccgg    12060

gtgtacaata tggacttcct cttttctggc aaccaaaccc atacatcggg attcctataa    12120

taccttcgtt ggtctcccta acatgtaggt ggcggagggg agatatacaa tagaacagat    12180

accagacaag acataatggg ctaaacaaaa ctacaccaat tacactgcct cattgatggt    12240

ggtacataac gaactaatac tgtagcccta gacttgatag ccatcatcat atcgaagttt    12300

cactaccctt tttccatttg ccatctattg aagtaataat aggcgcatg                12349


<210>  3
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Squence signal de localisation nuclaire

<400>  3

Pro Lys Lys Lys Arg Lys Val 
1               5           


<210>  4
<211>  20
<212>  PRT
<213>  VIH-I

<400>  4

Gly Arg Lys Lys Arg Arg Gln Arg Arg Arg Pro Pro Gln Pro Lys Lys 
1               5                   10                  15      


Lys Arg Lys Val 
            20  


<210>  5
<211>  19
<212>  PRT
<213>  Virus de l'hpatite B humaine

<400>  5

Pro Leu Ser Ser Ile Phe Ser Arg Ile Gly Asp Pro Pro Lys Lys Lys 
1               5                   10                  15      


Arg Lys Val 
            


<210>  6
<211>  1839
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protine de fusion comprenant la construction Cas9 sauvage -Spo11

<400>  6

Met Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr Gly 
1               5                   10                  15      


Gly Met Ala Pro Lys Lys Lys Arg Lys Val Asp Gly Gly Gly Ile His 
            20                  25                  30          


Gly Val Pro Ala Ala Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile 
        35                  40                  45              


Gly Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val 
    50                  55                  60                  


Pro Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile 
65                  70                  75                  80  


Lys Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala 
                85                  90                  95      


Glu Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg 
            100                 105                 110         


Lys Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala 
        115                 120                 125             


Lys Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val 
    130                 135                 140                 


Glu Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val 
145                 150                 155                 160 


Asp Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg 
                165                 170                 175     


Lys Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr 
            180                 185                 190         


Leu Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu 
        195                 200                 205             


Gly Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln 
    210                 215                 220                 


Leu Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala 
225                 230                 235                 240 


Ser Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser 
                245                 250                 255     


Arg Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn 
            260                 265                 270         


Gly Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn 
        275                 280                 285             


Phe Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser 
    290                 295                 300                 


Lys Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly 
305                 310                 315                 320 


Asp Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala 
                325                 330                 335     


Ile Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala 
            340                 345                 350         


Pro Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp 
        355                 360                 365             


Leu Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr 
    370                 375                 380                 


Lys Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile 
385                 390                 395                 400 


Asp Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile 
                405                 410                 415     


Leu Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg 
            420                 425                 430         


Glu Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro 
        435                 440                 445             


His Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu 
    450                 455                 460                 


Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile 
465                 470                 475                 480 


Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn 
                485                 490                 495     


Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro 
            500                 505                 510         


Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe 
        515                 520                 525             


Ile Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val 
    530                 535                 540                 


Leu Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu 
545                 550                 555                 560 


Leu Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe 
                565                 570                 575     


Leu Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr 
            580                 585                 590         


Asn Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys 
        595                 600                 605             


Ile Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe 
    610                 615                 620                 


Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp 
625                 630                 635                 640 


Lys Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile 
                645                 650                 655     


Val Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg 
            660                 665                 670         


Leu Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu 
        675                 680                 685             


Lys Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile 
    690                 695                 700                 


Asn Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu 
705                 710                 715                 720 


Lys Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp 
                725                 730                 735     


Asp Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly 
            740                 745                 750         


Gln Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro 
        755                 760                 765             


Ala Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu 
    770                 775                 780                 


Val Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met 
785                 790                 795                 800 


Ala Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu 
                805                 810                 815     


Arg Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile 
            820                 825                 830         


Leu Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu 
        835                 840                 845             


Tyr Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu 
    850                 855                 860                 


Leu Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro 
865                 870                 875                 880 


Gln Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg 
                885                 890                 895     


Ser Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val 
            900                 905                 910         


Val Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu 
        915                 920                 925             


Ile Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly 
    930                 935                 940                 


Leu Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu 
945                 950                 955                 960 


Thr Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met 
                965                 970                 975     


Asn Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val 
            980                 985                 990         


Ile Thr Leu Lys Ser Lys Leu Val  Ser Asp Phe Arg Lys  Asp Phe Gln 
        995                 1000                 1005             


Phe Tyr  Lys Val Arg Glu Ile  Asn Asn Tyr His His  Ala His Asp 
    1010                 1015                 1020             


Ala Tyr  Leu Asn Ala Val Val  Gly Thr Ala Leu Ile  Lys Lys Tyr 
    1025                 1030                 1035             


Pro Lys  Leu Glu Ser Glu Phe  Val Tyr Gly Asp Tyr  Lys Val Tyr 
    1040                 1045                 1050             


Asp Val  Arg Lys Met Ile Ala  Lys Ser Glu Gln Glu  Ile Gly Lys 
    1055                 1060                 1065             


Ala Thr  Ala Lys Tyr Phe Phe  Tyr Ser Asn Ile Met  Asn Phe Phe 
    1070                 1075                 1080             


Lys Thr  Glu Ile Thr Leu Ala  Asn Gly Glu Ile Arg  Lys Arg Pro 
    1085                 1090                 1095             


Leu Ile  Glu Thr Asn Gly Glu  Thr Gly Glu Ile Val  Trp Asp Lys 
    1100                 1105                 1110             


Gly Arg  Asp Phe Ala Thr Val  Arg Lys Val Leu Ser  Met Pro Gln 
    1115                 1120                 1125             


Val Asn  Ile Val Lys Lys Thr  Glu Val Gln Thr Gly  Gly Phe Ser 
    1130                 1135                 1140             


Lys Glu  Ser Ile Leu Pro Lys  Arg Asn Ser Asp Lys  Leu Ile Ala 
    1145                 1150                 1155             


Arg Lys  Lys Asp Trp Asp Pro  Lys Lys Tyr Gly Gly  Phe Asp Ser 
    1160                 1165                 1170             


Pro Thr  Val Ala Tyr Ser Val  Leu Val Val Ala Lys  Val Glu Lys 
    1175                 1180                 1185             


Gly Lys  Ser Lys Lys Leu Lys  Ser Val Lys Glu Leu  Leu Gly Ile 
    1190                 1195                 1200             


Thr Ile  Met Glu Arg Ser Ser  Phe Glu Lys Asn Pro  Ile Asp Phe 
    1205                 1210                 1215             


Leu Glu  Ala Lys Gly Tyr Lys  Glu Val Lys Lys Asp  Leu Ile Ile 
    1220                 1225                 1230             


Lys Leu  Pro Lys Tyr Ser Leu  Phe Glu Leu Glu Asn  Gly Arg Lys 
    1235                 1240                 1245             


Arg Met  Leu Ala Ser Ala Gly  Glu Leu Gln Lys Gly  Asn Glu Leu 
    1250                 1255                 1260             


Ala Leu  Pro Ser Lys Tyr Val  Asn Phe Leu Tyr Leu  Ala Ser His 
    1265                 1270                 1275             


Tyr Glu  Lys Leu Lys Gly Ser  Pro Glu Asp Asn Glu  Gln Lys Gln 
    1280                 1285                 1290             


Leu Phe  Val Glu Gln His Lys  His Tyr Leu Asp Glu  Ile Ile Glu 
    1295                 1300                 1305             


Gln Ile  Ser Glu Phe Ser Lys  Arg Val Ile Leu Ala  Asp Ala Asn 
    1310                 1315                 1320             


Leu Asp  Lys Val Leu Ser Ala  Tyr Asn Lys His Arg  Asp Lys Pro 
    1325                 1330                 1335             


Ile Arg  Glu Gln Ala Glu Asn  Ile Ile His Leu Phe  Thr Leu Thr 
    1340                 1345                 1350             


Asn Leu  Gly Ala Pro Ala Ala  Phe Lys Tyr Phe Asp  Thr Thr Ile 
    1355                 1360                 1365             


Asp Arg  Lys Arg Tyr Thr Ser  Thr Lys Glu Val Leu  Asp Ala Thr 
    1370                 1375                 1380             


Leu Ile  His Gln Ser Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp 
    1385                 1390                 1395             


Leu Ser  Gln Leu Gly Gly Asp  Pro Glu Phe Met Ala  Met Glu Ala 
    1400                 1405                 1410             


Pro Gly  Ile Arg Met Ala Leu  Glu Gly Leu Arg Lys  Lys Tyr Lys 
    1415                 1420                 1425             


Thr Arg  Gln Glu Leu Val Lys  Ala Leu Thr Pro Lys  Arg Arg Ser 
    1430                 1435                 1440             


Ile His  Leu Asn Ser Asn Gly  His Ser Asn Gly Thr  Pro Cys Ser 
    1445                 1450                 1455             


Asn Ala  Asp Val Leu Ala His  Ile Lys His Phe Leu  Ser Leu Ala 
    1460                 1465                 1470             


Ala Asn  Ser Leu Glu Gln His  Gln Gln Pro Ile Ser  Ile Val Phe 
    1475                 1480                 1485             


Gln Asn  Lys Lys Lys Lys Gly  Asp Thr Ser Ser Pro  Asp Ile His 
    1490                 1495                 1500             


Thr Thr  Leu Asp Phe Pro Leu  Asn Gly Pro His Leu  Cys Thr His 
    1505                 1510                 1515             


Gln Phe  Lys Leu Lys Arg Cys  Ala Ile Leu Leu Asn  Leu Leu Lys 
    1520                 1525                 1530             


Val Val  Met Glu Lys Leu Pro  Leu Gly Lys Asn Thr  Thr Val Arg 
    1535                 1540                 1545             


Asp Ile  Phe Tyr Ser Asn Val  Glu Leu Phe Gln Arg  Gln Ala Asn 
    1550                 1555                 1560             


Val Val  Gln Trp Leu Asp Val  Ile Arg Phe Asn Phe  Lys Leu Ser 
    1565                 1570                 1575             


Pro Arg  Lys Ser Leu Asn Ile  Ile Pro Ala Gln Lys  Gly Leu Val 
    1580                 1585                 1590             


Tyr Ser  Pro Phe Pro Ile Asp  Ile Tyr Asp Asn Ile  Leu Thr Cys 
    1595                 1600                 1605             


Glu Asn  Glu Pro Lys Met Gln  Lys Gln Thr Ile Phe  Pro Gly Lys 
    1610                 1615                 1620             


Pro Cys  Leu Ile Pro Phe Phe  Gln Asp Asp Ala Val  Ile Lys Leu 
    1625                 1630                 1635             


Gly Thr  Thr Ser Met Cys Asn  Ile Val Ile Val Glu  Lys Glu Ala 
    1640                 1645                 1650             


Val Phe  Thr Lys Leu Val Asn  Asn Tyr His Lys Leu  Ser Thr Asn 
    1655                 1660                 1665             


Thr Met  Leu Ile Thr Gly Lys  Gly Phe Pro Asp Phe  Leu Thr Arg 
    1670                 1675                 1680             


Leu Phe  Leu Lys Lys Leu Glu  Gln Tyr Cys Ser Lys  Leu Ile Ser 
    1685                 1690                 1695             


Asp Cys  Ser Ile Phe Thr Asp  Ala Asp Pro Tyr Gly  Ile Ser Ile 
    1700                 1705                 1710             


Ala Leu  Asn Tyr Thr His Ser  Asn Glu Arg Asn Ala  Tyr Ile Cys 
    1715                 1720                 1725             


Thr Met  Ala Asn Tyr Lys Gly  Ile Arg Ile Thr Gln  Val Leu Ala 
    1730                 1735                 1740             


Gln Asn  Asn Glu Val His Asn  Lys Ser Ile Gln Leu  Leu Ser Leu 
    1745                 1750                 1755             


Asn Gln  Arg Asp Tyr Ser Leu  Ala Lys Asn Leu Ile  Ala Ser Leu 
    1760                 1765                 1770             


Thr Ala  Asn Ser Trp Asp Ile  Ala Thr Ser Pro Leu  Lys Asn Val 
    1775                 1780                 1785             


Ile Ile  Glu Cys Gln Arg Glu  Ile Phe Phe Gln Lys  Lys Ala Glu 
    1790                 1795                 1800             


Met Asn  Glu Ile Asp Ala Arg  Ile Phe Glu Tyr Lys  Asp Tyr Lys 
    1805                 1810                 1815             


Asp Asp  Asp Asp Lys Asp Tyr  Lys Asp Asp Asp Asp  Lys Asp Tyr 
    1820                 1825                 1830             


Lys Asp  Asp Asp Asp Lys 
    1835                 


<210>  7
<211>  1839
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protine de fusion comprenant la construction Cas9*-Spo11

<400>  7

Met Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr Gly 
1               5                   10                  15      


Gly Met Ala Pro Lys Lys Lys Arg Lys Val Asp Gly Gly Gly Ile His 
            20                  25                  30          


Gly Val Pro Ala Ala Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile 
        35                  40                  45              


Gly Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val 
    50                  55                  60                  


Pro Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile 
65                  70                  75                  80  


Lys Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala 
                85                  90                  95      


Glu Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg 
            100                 105                 110         


Lys Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala 
        115                 120                 125             


Lys Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val 
    130                 135                 140                 


Glu Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val 
145                 150                 155                 160 


Asp Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg 
                165                 170                 175     


Lys Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr 
            180                 185                 190         


Leu Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu 
        195                 200                 205             


Gly Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln 
    210                 215                 220                 


Leu Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala 
225                 230                 235                 240 


Ser Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser 
                245                 250                 255     


Arg Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn 
            260                 265                 270         


Gly Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn 
        275                 280                 285             


Phe Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser 
    290                 295                 300                 


Lys Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly 
305                 310                 315                 320 


Asp Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala 
                325                 330                 335     


Ile Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala 
            340                 345                 350         


Pro Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp 
        355                 360                 365             


Leu Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr 
    370                 375                 380                 


Lys Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile 
385                 390                 395                 400 


Asp Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile 
                405                 410                 415     


Leu Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg 
            420                 425                 430         


Glu Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro 
        435                 440                 445             


His Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu 
    450                 455                 460                 


Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile 
465                 470                 475                 480 


Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn 
                485                 490                 495     


Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro 
            500                 505                 510         


Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe 
        515                 520                 525             


Ile Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val 
    530                 535                 540                 


Leu Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu 
545                 550                 555                 560 


Leu Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe 
                565                 570                 575     


Leu Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr 
            580                 585                 590         


Asn Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys 
        595                 600                 605             


Ile Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe 
    610                 615                 620                 


Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp 
625                 630                 635                 640 


Lys Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile 
                645                 650                 655     


Val Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg 
            660                 665                 670         


Leu Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu 
        675                 680                 685             


Lys Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile 
    690                 695                 700                 


Asn Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu 
705                 710                 715                 720 


Lys Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp 
                725                 730                 735     


Asp Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly 
            740                 745                 750         


Gln Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro 
        755                 760                 765             


Ala Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu 
    770                 775                 780                 


Val Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met 
785                 790                 795                 800 


Ala Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu 
                805                 810                 815     


Arg Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile 
            820                 825                 830         


Leu Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu 
        835                 840                 845             


Tyr Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu 
    850                 855                 860                 


Leu Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro 
865                 870                 875                 880 


Gln Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg 
                885                 890                 895     


Ser Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val 
            900                 905                 910         


Val Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu 
        915                 920                 925             


Ile Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly 
    930                 935                 940                 


Leu Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu 
945                 950                 955                 960 


Thr Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met 
                965                 970                 975     


Asn Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val 
            980                 985                 990         


Ile Thr Leu Lys Ser Lys Leu Val  Ser Asp Phe Arg Lys  Asp Phe Gln 
        995                 1000                 1005             


Phe Tyr  Lys Val Arg Glu Ile  Asn Asn Tyr His His  Ala His Asp 
    1010                 1015                 1020             


Ala Tyr  Leu Asn Ala Val Val  Gly Thr Ala Leu Ile  Lys Lys Tyr 
    1025                 1030                 1035             


Pro Lys  Leu Glu Ser Glu Phe  Val Tyr Gly Asp Tyr  Lys Val Tyr 
    1040                 1045                 1050             


Asp Val  Arg Lys Met Ile Ala  Lys Ser Glu Gln Glu  Ile Gly Lys 
    1055                 1060                 1065             


Ala Thr  Ala Lys Tyr Phe Phe  Tyr Ser Asn Ile Met  Asn Phe Phe 
    1070                 1075                 1080             


Lys Thr  Glu Ile Thr Leu Ala  Asn Gly Glu Ile Arg  Lys Arg Pro 
    1085                 1090                 1095             


Leu Ile  Glu Thr Asn Gly Glu  Thr Gly Glu Ile Val  Trp Asp Lys 
    1100                 1105                 1110             


Gly Arg  Asp Phe Ala Thr Val  Arg Lys Val Leu Ser  Met Pro Gln 
    1115                 1120                 1125             


Val Asn  Ile Val Lys Lys Thr  Glu Val Gln Thr Gly  Gly Phe Ser 
    1130                 1135                 1140             


Lys Glu  Ser Ile Leu Pro Lys  Arg Asn Ser Asp Lys  Leu Ile Ala 
    1145                 1150                 1155             


Arg Lys  Lys Asp Trp Asp Pro  Lys Lys Tyr Gly Gly  Phe Asp Ser 
    1160                 1165                 1170             


Pro Thr  Val Ala Tyr Ser Val  Leu Val Val Ala Lys  Val Glu Lys 
    1175                 1180                 1185             


Gly Lys  Ser Lys Lys Leu Lys  Ser Val Lys Glu Leu  Leu Gly Ile 
    1190                 1195                 1200             


Thr Ile  Met Glu Arg Ser Ser  Phe Glu Lys Asn Pro  Ile Asp Phe 
    1205                 1210                 1215             


Leu Glu  Ala Lys Gly Tyr Lys  Glu Val Lys Lys Asp  Leu Ile Ile 
    1220                 1225                 1230             


Lys Leu  Pro Lys Tyr Ser Leu  Phe Glu Leu Glu Asn  Gly Arg Lys 
    1235                 1240                 1245             


Arg Met  Leu Ala Ser Ala Gly  Glu Leu Gln Lys Gly  Asn Glu Leu 
    1250                 1255                 1260             


Ala Leu  Pro Ser Lys Tyr Val  Asn Phe Leu Tyr Leu  Ala Ser His 
    1265                 1270                 1275             


Tyr Glu  Lys Leu Lys Gly Ser  Pro Glu Asp Asn Glu  Gln Lys Gln 
    1280                 1285                 1290             


Leu Phe  Val Glu Gln His Lys  His Tyr Leu Asp Glu  Ile Ile Glu 
    1295                 1300                 1305             


Gln Ile  Ser Glu Phe Ser Lys  Arg Val Ile Leu Ala  Asp Ala Asn 
    1310                 1315                 1320             


Leu Asp  Lys Val Leu Ser Ala  Tyr Asn Lys His Arg  Asp Lys Pro 
    1325                 1330                 1335             


Ile Arg  Glu Gln Ala Glu Asn  Ile Ile His Leu Phe  Thr Leu Thr 
    1340                 1345                 1350             


Asn Leu  Gly Ala Pro Ala Ala  Phe Lys Tyr Phe Asp  Thr Thr Ile 
    1355                 1360                 1365             


Asp Arg  Lys Arg Tyr Thr Ser  Thr Lys Glu Val Leu  Asp Ala Thr 
    1370                 1375                 1380             


Leu Ile  His Gln Ser Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp 
    1385                 1390                 1395             


Leu Ser  Gln Leu Gly Gly Asp  Pro Glu Phe Met Ala  Met Glu Ala 
    1400                 1405                 1410             


Pro Gly  Ile Arg Met Ala Leu  Glu Gly Leu Arg Lys  Lys Tyr Lys 
    1415                 1420                 1425             


Thr Arg  Gln Glu Leu Val Lys  Ala Leu Thr Pro Lys  Arg Arg Ser 
    1430                 1435                 1440             


Ile His  Leu Asn Ser Asn Gly  His Ser Asn Gly Thr  Pro Cys Ser 
    1445                 1450                 1455             


Asn Ala  Asp Val Leu Ala His  Ile Lys His Phe Leu  Ser Leu Ala 
    1460                 1465                 1470             


Ala Asn  Ser Leu Glu Gln His  Gln Gln Pro Ile Ser  Ile Val Phe 
    1475                 1480                 1485             


Gln Asn  Lys Lys Lys Lys Gly  Asp Thr Ser Ser Pro  Asp Ile His 
    1490                 1495                 1500             


Thr Thr  Leu Asp Phe Pro Leu  Asn Gly Pro His Leu  Cys Thr His 
    1505                 1510                 1515             


Gln Phe  Lys Leu Lys Arg Cys  Ala Ile Leu Leu Asn  Leu Leu Lys 
    1520                 1525                 1530             


Val Val  Met Glu Lys Leu Pro  Leu Gly Lys Asn Thr  Thr Val Arg 
    1535                 1540                 1545             


Asp Ile  Phe Tyr Ser Asn Val  Glu Leu Phe Gln Arg  Gln Ala Asn 
    1550                 1555                 1560             


Val Val  Gln Trp Leu Asp Val  Ile Arg Phe Asn Phe  Lys Leu Ser 
    1565                 1570                 1575             


Pro Arg  Lys Ser Leu Asn Ile  Ile Pro Ala Gln Lys  Gly Leu Val 
    1580                 1585                 1590             


Tyr Ser  Pro Phe Pro Ile Asp  Ile Tyr Asp Asn Ile  Leu Thr Cys 
    1595                 1600                 1605             


Glu Asn  Glu Pro Lys Met Gln  Lys Gln Thr Ile Phe  Pro Gly Lys 
    1610                 1615                 1620             


Pro Cys  Leu Ile Pro Phe Phe  Gln Asp Asp Ala Val  Ile Lys Leu 
    1625                 1630                 1635             


Gly Thr  Thr Ser Met Cys Asn  Ile Val Ile Val Glu  Lys Glu Ala 
    1640                 1645                 1650             


Val Phe  Thr Lys Leu Val Asn  Asn Tyr His Lys Leu  Ser Thr Asn 
    1655                 1660                 1665             


Thr Met  Leu Ile Thr Gly Lys  Gly Phe Pro Asp Phe  Leu Thr Arg 
    1670                 1675                 1680             


Leu Phe  Leu Lys Lys Leu Glu  Gln Tyr Cys Ser Lys  Leu Ile Ser 
    1685                 1690                 1695             


Asp Cys  Ser Ile Phe Thr Asp  Ala Asp Pro Tyr Gly  Ile Ser Ile 
    1700                 1705                 1710             


Ala Leu  Asn Tyr Thr His Ser  Asn Glu Arg Asn Ala  Tyr Ile Cys 
    1715                 1720                 1725             


Thr Met  Ala Asn Tyr Lys Gly  Ile Arg Ile Thr Gln  Val Leu Ala 
    1730                 1735                 1740             


Gln Asn  Asn Glu Val His Asn  Lys Ser Ile Gln Leu  Leu Ser Leu 
    1745                 1750                 1755             


Asn Gln  Arg Asp Tyr Ser Leu  Ala Lys Asn Leu Ile  Ala Ser Leu 
    1760                 1765                 1770             


Thr Ala  Asn Ser Trp Asp Ile  Ala Thr Ser Pro Leu  Lys Asn Val 
    1775                 1780                 1785             


Ile Ile  Glu Cys Gln Arg Glu  Ile Phe Phe Gln Lys  Lys Ala Glu 
    1790                 1795                 1800             


Met Asn  Glu Ile Asp Ala Arg  Ile Phe Glu Tyr Lys  Asp Tyr Lys 
    1805                 1810                 1815             


Asp Asp  Asp Asp Lys Asp Tyr  Lys Asp Asp Asp Asp  Lys Asp Tyr 
    1820                 1825                 1830             


Lys Asp  Asp Asp Asp Lys 
    1835                 


<210>  8
<211>  1368
<212>  PRT
<213>  Streptococcus pyogenes

<400>  8

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


<210>  9
<211>  398
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  9

Met Ala Leu Glu Gly Leu Arg Lys Lys Tyr Lys Thr Arg Gln Glu Leu 
1               5                   10                  15      


Val Lys Ala Leu Thr Pro Lys Arg Arg Ser Ile His Leu Asn Ser Asn 
            20                  25                  30          


Gly His Ser Asn Gly Thr Pro Cys Ser Asn Ala Asp Val Leu Ala His 
        35                  40                  45              


Ile Lys His Phe Leu Ser Leu Ala Ala Asn Ser Leu Glu Gln His Gln 
    50                  55                  60                  


Gln Pro Ile Ser Ile Val Phe Gln Asn Lys Lys Lys Lys Gly Asp Thr 
65                  70                  75                  80  


Ser Ser Pro Asp Ile His Thr Thr Leu Asp Phe Pro Leu Asn Gly Pro 
                85                  90                  95      


His Leu Cys Thr His Gln Phe Lys Leu Lys Arg Cys Ala Ile Leu Leu 
            100                 105                 110         


Asn Leu Leu Lys Val Val Met Glu Lys Leu Pro Leu Gly Lys Asn Thr 
        115                 120                 125             


Thr Val Arg Asp Ile Phe Tyr Ser Asn Val Glu Leu Phe Gln Arg Gln 
    130                 135                 140                 


Ala Asn Val Val Gln Trp Leu Asp Val Ile Arg Phe Asn Phe Lys Leu 
145                 150                 155                 160 


Ser Pro Arg Lys Ser Leu Asn Ile Ile Pro Ala Gln Lys Gly Leu Val 
                165                 170                 175     


Tyr Ser Pro Phe Pro Ile Asp Ile Tyr Asp Asn Ile Leu Thr Cys Glu 
            180                 185                 190         


Asn Glu Pro Lys Met Gln Lys Gln Thr Ile Phe Pro Gly Lys Pro Cys 
        195                 200                 205             


Leu Ile Pro Phe Phe Gln Asp Asp Ala Val Ile Lys Leu Gly Thr Thr 
    210                 215                 220                 


Ser Met Cys Asn Ile Val Ile Val Glu Lys Glu Ala Val Phe Thr Lys 
225                 230                 235                 240 


Leu Val Asn Asn Tyr His Lys Leu Ser Thr Asn Thr Met Leu Ile Thr 
                245                 250                 255     


Gly Lys Gly Phe Pro Asp Phe Leu Thr Arg Leu Phe Leu Lys Lys Leu 
            260                 265                 270         


Glu Gln Tyr Cys Ser Lys Leu Ile Ser Asp Cys Ser Ile Phe Thr Asp 
        275                 280                 285             


Ala Asp Pro Tyr Gly Ile Ser Ile Ala Leu Asn Tyr Thr His Ser Asn 
    290                 295                 300                 


Glu Arg Asn Ala Tyr Ile Cys Thr Met Ala Asn Tyr Lys Gly Ile Arg 
305                 310                 315                 320 


Ile Thr Gln Val Leu Ala Gln Asn Asn Glu Val His Asn Lys Ser Ile 
                325                 330                 335     


Gln Leu Leu Ser Leu Asn Gln Arg Asp Tyr Ser Leu Ala Lys Asn Leu 
            340                 345                 350         


Ile Ala Ser Leu Thr Ala Asn Ser Trp Asp Ile Ala Thr Ser Pro Leu 
        355                 360                 365             


Lys Asn Val Ile Ile Glu Cys Gln Arg Glu Ile Phe Phe Gln Lys Lys 
    370                 375                 380                 


Ala Glu Met Asn Glu Ile Asp Ala Arg Ile Phe Glu Tyr Lys 
385                 390                 395             


<210>  10
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ARN guide UAS1 ciblant la rgion codante en 5' du gne YCR048W

<400>  10
cacacctgaa gagcaagtct gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tt                         102


<210>  11
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ARN guide UAS2 ciblant la rgion codante en 3' du gne YCR048W

<400>  11
ttccacaaca cgcccacctt gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tt                         102


<210>  12
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ARN guide UAS D/E ciblant le site du promoteur du gne GAL2

<400>  12
gatcactccg aaccgagatt gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tt                         102


<210>  13
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ARN guide SWC3 ciblant la rgion codante du gne SWC3

<400>  13
tgcgattgtg taatgagtgg gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tt                         102


<210>  14
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ARN guide UAS A ciblant le site du promoteur de GAL2

<400>  14
caattcggaa agcttccttc gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tt                         102


<210>  15
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ARN guide UAS B ciblant le site du promoteur du gne GAL2

<400>  15
ttgcctcagg aaggcaccgg gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tt                         102


<210>  16
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ARN guide PUT4 ciblant la rgion codante du gne PUT4

<400>  16
gttttgaatt ccgatcacaa gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tt                         102


<210>  17
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  linker de la construction dCas9-SPO11 pour la transformation de 
       cellules de riz

<400>  17
ccggaattta tggccatgga ggccccgggg atccgt                                 36


<210>  18
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Linker de la construction dCas9-SPO11 pour la transformation de 
       cellule de riz

<400>  18
ggtattcatg gagttcctgc tgcg                                              24


