SEQUENCE LISTING

<110>  CELLECTIS; RINAT

<120>  ANTI-HSP70 SPECIFIC CHIMERIC ANTIGEN RECEPTORS (CARS) FOR CANCER IMMUNOTHERAPY

<130>  P81600413PCT00

<150>  PA201570044
<151>  2015-01-26

<160> 47
<170>  PatentIn version 3.5


<210>  1

<211>  20
<212>  PRT
<213>  artificial sequence

<220>
<223>  signal peptide

<400>  1
Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly 
            20  


<210>  2

<211>  21
<212>  PRT
<213>  artificial sequence

<220>
<223>  signal peptide

<400>  2
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


<210>  3

<211>  16
<212>  PRT
<213>  homo sapiens

<220>
<223>  FcgRIIIa hinge

<400>  3
Gly Leu Ala Val Ser Thr Ile Ser Ser Phe Phe Pro Pro Gly Tyr Gln 
1               5                   10                  15      



<210>  4

<211>  45
<212>  PRT
<213>  homo sapiens

<220>
<223>  CD8alpha hinge

<400>  4
Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        35                  40                  45  


<210>  5

<211>  231
<212>  PRT
<213>  homo sapiens

<220>
<223>  IgG1 hinge

<400>  5
Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
1               5                   10                  15      


Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
            20                  25                  30          


Asp Thr Leu Met Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val Val 
        35                  40                  45              


Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 
    50                  55                  60                  


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 
65                  70                  75                  80  


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
                85                  90                  95      


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 
            100                 105                 110         


Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
        115                 120                 125             


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys 
    130                 135                 140                 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
145                 150                 155                 160 


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
                165                 170                 175     


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
            180                 185                 190         


Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 
        195                 200                 205             


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
    210                 215                 220                 


Leu Ser Leu Ser Pro Gly Lys 
225                 230     


<210>  6

<211>  24
<212>  PRT
<213>  homo sapiens

<220>
<223>  CD8alpha transmembrane domain

<400>  6
Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr Leu Tyr Cys 
            20                  


<210>  7

<211>  27
<212>  PRT
<213>  homo sapiens

<220>
<223>  41BB transmembrane domain

<400>  7
Ile Ile Ser Phe Phe Leu Ala Leu Thr Ser Thr Ala Leu Leu Phe Leu 
1               5                   10                  15      


Leu Phe Phe Leu Thr Leu Arg Phe Ser Val Val 
            20                  25          


<210>  8

<211>  42
<212>  PRT
<213>  homo sapiens

<220>
<223>  Fragment of 4-1BB (residues 214-255)

<400>  8
Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210>  9

<211>  112
<212>  PRT
<213>  homo sapiens

<220>
<223>  fragment of T-cell surface glycoprotein CD3 zeta chain

<400>  9
Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  10

<211>  15
<212>  PRT
<213>  artificial sequence

<220>
<223>  GS linker

<400>  10
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  11

<211>  117
<212>  PRT
<213>  artificial sequence

<220>
<223>  Murine cmHsp70.1 heavy chain variable region

<400>  11
Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln 
1               5                   10                  15      


Ser Leu Ser Phe Thr Cys Thr Val Ser Gly Phe Ser Leu Ser Arg Asn 
            20                  25                  30          


Ser Val His Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              


Gly Met Ile Trp Gly Gly Gly Ser Thr Asp Tyr Asn Ser Ala Leu Lys 
    50                  55                  60                  


Ser Arg Leu Asn Ile Ser Lys Asp Ser Ser Lys Ser Gln Val Phe Leu 
65                  70                  75                  80  


Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Met Tyr Phe Cys Ala 
                85                  90                  95      


Arg Asn Gly Gly Tyr Asp Val Phe His Tyr Trp Gly Gln Gly Thr Thr 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  12

<211>  117
<212>  PRT
<213>  artificial sequence

<220>
<223>  Humanized cmHsp70.1 heavy chain variable region

<400>  12
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ser Leu Ser Arg Asn 
            20                  25                  30          


Ser Val His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              


Gly Met Ile Trp Gly Gly Gly Ser Thr Asp Tyr Asn Ser Ala Leu Lys 
    50                  55                  60                  


Ser Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Asn Gly Gly Tyr Asp Val Phe His Tyr Trp Gly Gln Gly Thr Thr 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  13

<211>  10
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR1 of cmHsp70.1 heavy chain variable region

<400>  13
Gly Phe Ser Leu Ser Arg Asn Ser Val His 
1               5                   10  


<210>  14

<211>  19
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR2 of cmHsp70.1 heavy chain variable region

<400>  14
Trp Leu Gly Met Ile Trp Gly Gly Gly Ser Thr Asp Tyr Asn Ser Ala 
1               5                   10                  15      


Leu Lys Ser 
            


<210>  15

<211>  9
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR3 of cmHsp70.1 heavy chain variable region

<400>  15
Asn Gly Gly Tyr Asp Val Phe His Tyr 
1               5                   


<210>  16

<211>  110
<212>  PRT
<213>  artificial sequence

<220>
<223>  Murine cmHsp70.1 light chain variable region

<400>  16
Gln Ala Val Val Thr Gln Glu Ser Ala Leu Thr Thr Ser Pro Gly Glu 
1               5                   10                  15      


Thr Val Thr Leu Thr Cys Arg Ser Ser Thr Gly Ala Val Thr Thr Ser 
            20                  25                  30          


Asn Tyr Ala Asn Trp Val Gln Glu Lys Pro Asp His Leu Phe Thr Gly 
        35                  40                  45              


Leu Ile Gly Gly Thr Asn Asn Arg Ala Pro Gly Val Pro Ala Arg Phe 
    50                  55                  60                  


Ser Gly Ser Leu Ile Gly Asp Lys Ala Ala Leu Thr Ile Thr Gly Ala 
65                  70                  75                  80  


Gln Thr Glu Asp Glu Ala Ile Tyr Phe Cys Ala Leu Trp Tyr Ser Asn 
                85                  90                  95      


His Leu Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly 
            100                 105                 110 


<210>  17

<211>  110
<212>  PRT
<213>  artificial sequence

<220>
<223>  Humanized cmHsp70.1 light chain variable region

<400>  17
Gln Ala Val Val Thr Gln Glu Pro Ser Leu Thr Val Ser Pro Gly Gly 
1               5                   10                  15      


Thr Val Thr Leu Thr Cys Arg Ser Ser Thr Gly Ala Val Thr Thr Ser 
            20                  25                  30          


Asn Tyr Ala Asn Trp Val Gln Gln Lys Pro Gly Gln Ala Pro Arg Gly 
        35                  40                  45              


Leu Ile Gly Gly Thr Asn Asn Arg Ala Pro Trp Thr Pro Ala Arg Phe 
    50                  55                  60                  


Ser Gly Ser Leu Leu Gly Gly Lys Ala Ala Leu Thr Leu Ser Gly Val 
65                  70                  75                  80  


Gln Pro Glu Asp Glu Ala Glu Tyr Tyr Cys Ala Leu Trp Tyr Ser Asn 
                85                  90                  95      


His Leu Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly 
            100                 105                 110 


<210>  18

<211>  16
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR1 of cmHsp70.1 light chain variable region

<400>  18
Arg Ser Ser Thr Gly Ala Val Thr Thr Ser Asn Tyr Ala Asn Trp Val 
1               5                   10                  15      


<210>  19

<211>  11
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR2 of cmHsp70.1 light chain variable region

<400>  19
Gly Leu Ile Gly Gly Thr Asn Asn Arg Ala Pro 
1               5                   10      


<210>  20

<211>  9
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR3 of cmHsp70.1 light chain variable region

<400>  20
Ala Leu Trp Tyr Ser Asn His Leu Val 
1               5                   


<210>  21

<211>  457
<212>  PRT
<213>  artificial sequence

<220>
<223>  V1 mouse cmHsp70.1 scCAR polypeptide

<400>  21
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu 
            20                  25                  30          


Val Ala Pro Ser Gln Ser Leu Ser Phe Thr Cys Thr Val Ser Gly Phe 
        35                  40                  45              


Ser Leu Ser Arg Asn Ser Val His Trp Val Arg Gln Pro Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Gly Gly Ser Thr Asp Tyr 
65                  70                  75                  80  


Asn Ser Ala Leu Lys Ser Arg Leu Asn Ile Ser Lys Asp Ser Ser Lys 
                85                  90                  95      


Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala 
            100                 105                 110         


Met Tyr Phe Cys Ala Arg Asn Gly Gly Tyr Asp Val Phe His Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Ala Val Val Thr Gln Glu 
145                 150                 155                 160 


Ser Ala Leu Thr Thr Ser Pro Gly Glu Thr Val Thr Leu Thr Cys Arg 
                165                 170                 175     


Ser Ser Thr Gly Ala Val Thr Thr Ser Asn Tyr Ala Asn Trp Val Gln 
            180                 185                 190         


Glu Lys Pro Asp His Leu Phe Thr Gly Leu Ile Gly Gly Thr Asn Asn 
        195                 200                 205             


Arg Ala Pro Gly Val Pro Ala Arg Phe Ser Gly Ser Leu Ile Gly Asp 
    210                 215                 220                 


Lys Ala Ala Leu Thr Ile Thr Gly Ala Gln Thr Glu Asp Glu Ala Ile 
225                 230                 235                 240 


Tyr Phe Cys Ala Leu Trp Tyr Ser Asn His Leu Val Phe Gly Gly Gly 
                245                 250                 255     


Thr Lys Leu Thr Val Leu Gly Gly Leu Ala Val Ser Thr Ile Ser Ser 
            260                 265                 270         


Phe Phe Pro Pro Gly Tyr Gln Ile Tyr Ile Trp Ala Pro Leu Ala Gly 
        275                 280                 285             


Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys 
    290                 295                 300                 


Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg 
305                 310                 315                 320 


Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro 
                325                 330                 335     


Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser 
            340                 345                 350         


Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
        355                 360                 365             


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
    370                 375                 380                 


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
385                 390                 395                 400 


Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
                405                 410                 415     


Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
            420                 425                 430         


Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
        435                 440                 445             


Leu His Met Gln Ala Leu Pro Pro Arg 
    450                 455         


<210>  22

<211>  460
<212>  PRT
<213>  artificial sequence

<220>
<223>  V2 mouse cmHsp70.1 scCAR polypeptide

<400>  22
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu 
            20                  25                  30          


Val Ala Pro Ser Gln Ser Leu Ser Phe Thr Cys Thr Val Ser Gly Phe 
        35                  40                  45              


Ser Leu Ser Arg Asn Ser Val His Trp Val Arg Gln Pro Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Gly Gly Ser Thr Asp Tyr 
65                  70                  75                  80  


Asn Ser Ala Leu Lys Ser Arg Leu Asn Ile Ser Lys Asp Ser Ser Lys 
                85                  90                  95      


Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala 
            100                 105                 110         


Met Tyr Phe Cys Ala Arg Asn Gly Gly Tyr Asp Val Phe His Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Ala Val Val Thr Gln Glu 
145                 150                 155                 160 


Ser Ala Leu Thr Thr Ser Pro Gly Glu Thr Val Thr Leu Thr Cys Arg 
                165                 170                 175     


Ser Ser Thr Gly Ala Val Thr Thr Ser Asn Tyr Ala Asn Trp Val Gln 
            180                 185                 190         


Glu Lys Pro Asp His Leu Phe Thr Gly Leu Ile Gly Gly Thr Asn Asn 
        195                 200                 205             


Arg Ala Pro Gly Val Pro Ala Arg Phe Ser Gly Ser Leu Ile Gly Asp 
    210                 215                 220                 


Lys Ala Ala Leu Thr Ile Thr Gly Ala Gln Thr Glu Asp Glu Ala Ile 
225                 230                 235                 240 


Tyr Phe Cys Ala Leu Trp Tyr Ser Asn His Leu Val Phe Gly Gly Gly 
                245                 250                 255     


Thr Lys Leu Thr Val Leu Gly Gly Leu Ala Val Ser Thr Ile Ser Ser 
            260                 265                 270         


Phe Phe Pro Pro Gly Tyr Gln Ile Ile Ser Phe Phe Leu Ala Leu Thr 
        275                 280                 285             


Ser Thr Ala Leu Leu Phe Leu Leu Phe Phe Leu Thr Leu Arg Phe Ser 
    290                 295                 300                 


Val Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro 
305                 310                 315                 320 


Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys 
                325                 330                 335     


Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe 
            340                 345                 350         


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu 
        355                 360                 365             


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
    370                 375                 380                 


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys 
385                 390                 395                 400 


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
                405                 410                 415     


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
            420                 425                 430         


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
        435                 440                 445             


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
    450                 455                 460 


<210>  23

<211>  486
<212>  PRT
<213>  artificial sequence

<220>
<223>  V3 mouse cmHsp70.1 scCAR polypeptide

<400>  23
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu 
            20                  25                  30          


Val Ala Pro Ser Gln Ser Leu Ser Phe Thr Cys Thr Val Ser Gly Phe 
        35                  40                  45              


Ser Leu Ser Arg Asn Ser Val His Trp Val Arg Gln Pro Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Gly Gly Ser Thr Asp Tyr 
65                  70                  75                  80  


Asn Ser Ala Leu Lys Ser Arg Leu Asn Ile Ser Lys Asp Ser Ser Lys 
                85                  90                  95      


Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala 
            100                 105                 110         


Met Tyr Phe Cys Ala Arg Asn Gly Gly Tyr Asp Val Phe His Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Ala Val Val Thr Gln Glu 
145                 150                 155                 160 


Ser Ala Leu Thr Thr Ser Pro Gly Glu Thr Val Thr Leu Thr Cys Arg 
                165                 170                 175     


Ser Ser Thr Gly Ala Val Thr Thr Ser Asn Tyr Ala Asn Trp Val Gln 
            180                 185                 190         


Glu Lys Pro Asp His Leu Phe Thr Gly Leu Ile Gly Gly Thr Asn Asn 
        195                 200                 205             


Arg Ala Pro Gly Val Pro Ala Arg Phe Ser Gly Ser Leu Ile Gly Asp 
    210                 215                 220                 


Lys Ala Ala Leu Thr Ile Thr Gly Ala Gln Thr Glu Asp Glu Ala Ile 
225                 230                 235                 240 


Tyr Phe Cys Ala Leu Trp Tyr Ser Asn His Leu Val Phe Gly Gly Gly 
                245                 250                 255     


Thr Lys Leu Thr Val Leu Gly Thr Thr Thr Pro Ala Pro Arg Pro Pro 
            260                 265                 270         


Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu 
        275                 280                 285             


Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp 
    290                 295                 300                 


Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly 
305                 310                 315                 320 


Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg 
                325                 330                 335     


Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln 
            340                 345                 350         


Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu 
        355                 360                 365             


Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala 
    370                 375                 380                 


Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu 
385                 390                 395                 400 


Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp 
                405                 410                 415     


Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu 
            420                 425                 430         


Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile 
        435                 440                 445             


Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr 
    450                 455                 460                 


Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met 
465                 470                 475                 480 


Gln Ala Leu Pro Pro Arg 
                485     


<210>  24

<211>  489
<212>  PRT
<213>  artificial sequence

<220>
<223>  V4 mouse cmHsp70.1 scCAR polypeptide

<400>  24
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu 
            20                  25                  30          


Val Ala Pro Ser Gln Ser Leu Ser Phe Thr Cys Thr Val Ser Gly Phe 
        35                  40                  45              


Ser Leu Ser Arg Asn Ser Val His Trp Val Arg Gln Pro Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Gly Gly Ser Thr Asp Tyr 
65                  70                  75                  80  


Asn Ser Ala Leu Lys Ser Arg Leu Asn Ile Ser Lys Asp Ser Ser Lys 
                85                  90                  95      


Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala 
            100                 105                 110         


Met Tyr Phe Cys Ala Arg Asn Gly Gly Tyr Asp Val Phe His Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Ala Val Val Thr Gln Glu 
145                 150                 155                 160 


Ser Ala Leu Thr Thr Ser Pro Gly Glu Thr Val Thr Leu Thr Cys Arg 
                165                 170                 175     


Ser Ser Thr Gly Ala Val Thr Thr Ser Asn Tyr Ala Asn Trp Val Gln 
            180                 185                 190         


Glu Lys Pro Asp His Leu Phe Thr Gly Leu Ile Gly Gly Thr Asn Asn 
        195                 200                 205             


Arg Ala Pro Gly Val Pro Ala Arg Phe Ser Gly Ser Leu Ile Gly Asp 
    210                 215                 220                 


Lys Ala Ala Leu Thr Ile Thr Gly Ala Gln Thr Glu Asp Glu Ala Ile 
225                 230                 235                 240 


Tyr Phe Cys Ala Leu Trp Tyr Ser Asn His Leu Val Phe Gly Gly Gly 
                245                 250                 255     


Thr Lys Leu Thr Val Leu Gly Thr Thr Thr Pro Ala Pro Arg Pro Pro 
            260                 265                 270         


Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu 
        275                 280                 285             


Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp 
    290                 295                 300                 


Phe Ala Cys Asp Ile Ile Ser Phe Phe Leu Ala Leu Thr Ser Thr Ala 
305                 310                 315                 320 


Leu Leu Phe Leu Leu Phe Phe Leu Thr Leu Arg Phe Ser Val Val Lys 
                325                 330                 335     


Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg 
            340                 345                 350         


Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro 
        355                 360                 365             


Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser 
    370                 375                 380                 


Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
385                 390                 395                 400 


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
                405                 410                 415     


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
            420                 425                 430         


Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
        435                 440                 445             


Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
    450                 455                 460                 


Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
465                 470                 475                 480 


Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 


<210>  25

<211>  672
<212>  PRT
<213>  artificial sequence

<220>
<223>  V5 mouse cmHsp70.1 scCAR polypeptide

<400>  25
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu 
            20                  25                  30          


Val Ala Pro Ser Gln Ser Leu Ser Phe Thr Cys Thr Val Ser Gly Phe 
        35                  40                  45              


Ser Leu Ser Arg Asn Ser Val His Trp Val Arg Gln Pro Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Gly Gly Ser Thr Asp Tyr 
65                  70                  75                  80  


Asn Ser Ala Leu Lys Ser Arg Leu Asn Ile Ser Lys Asp Ser Ser Lys 
                85                  90                  95      


Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala 
            100                 105                 110         


Met Tyr Phe Cys Ala Arg Asn Gly Gly Tyr Asp Val Phe His Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Ala Val Val Thr Gln Glu 
145                 150                 155                 160 


Ser Ala Leu Thr Thr Ser Pro Gly Glu Thr Val Thr Leu Thr Cys Arg 
                165                 170                 175     


Ser Ser Thr Gly Ala Val Thr Thr Ser Asn Tyr Ala Asn Trp Val Gln 
            180                 185                 190         


Glu Lys Pro Asp His Leu Phe Thr Gly Leu Ile Gly Gly Thr Asn Asn 
        195                 200                 205             


Arg Ala Pro Gly Val Pro Ala Arg Phe Ser Gly Ser Leu Ile Gly Asp 
    210                 215                 220                 


Lys Ala Ala Leu Thr Ile Thr Gly Ala Gln Thr Glu Asp Glu Ala Ile 
225                 230                 235                 240 


Tyr Phe Cys Ala Leu Trp Tyr Ser Asn His Leu Val Phe Gly Gly Gly 
                245                 250                 255     


Thr Lys Leu Thr Val Leu Gly Glu Pro Lys Ser Pro Asp Lys Thr His 
            260                 265                 270         


Thr Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser Val Phe 
        275                 280                 285             


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ala Arg Thr Pro 
    290                 295                 300                 


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
305                 310                 315                 320 


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
                325                 330                 335     


Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 
            340                 345                 350         


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
        355                 360                 365             


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
    370                 375                 380                 


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
385                 390                 395                 400 


Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
                405                 410                 415     


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
            420                 425                 430         


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
        435                 440                 445             


Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
    450                 455                 460                 


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
465                 470                 475                 480 


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Ile Tyr 
                485                 490                 495     


Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu 
            500                 505                 510         


Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile 
        515                 520                 525             


Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp 
    530                 535                 540                 


Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
545                 550                 555                 560 


Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
                565                 570                 575     


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            580                 585                 590         


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        595                 600                 605             


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    610                 615                 620                 


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
625                 630                 635                 640 


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                645                 650                 655     


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            660                 665                 670         


<210>  26

<211>  675
<212>  PRT
<213>  artificial sequence

<220>
<223>  V6 mouse cmHsp70.1 scCAR polypeptide

<400>  26
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu 
            20                  25                  30          


Val Ala Pro Ser Gln Ser Leu Ser Phe Thr Cys Thr Val Ser Gly Phe 
        35                  40                  45              


Ser Leu Ser Arg Asn Ser Val His Trp Val Arg Gln Pro Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Gly Gly Ser Thr Asp Tyr 
65                  70                  75                  80  


Asn Ser Ala Leu Lys Ser Arg Leu Asn Ile Ser Lys Asp Ser Ser Lys 
                85                  90                  95      


Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala 
            100                 105                 110         


Met Tyr Phe Cys Ala Arg Asn Gly Gly Tyr Asp Val Phe His Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Ala Val Val Thr Gln Glu 
145                 150                 155                 160 


Ser Ala Leu Thr Thr Ser Pro Gly Glu Thr Val Thr Leu Thr Cys Arg 
                165                 170                 175     


Ser Ser Thr Gly Ala Val Thr Thr Ser Asn Tyr Ala Asn Trp Val Gln 
            180                 185                 190         


Glu Lys Pro Asp His Leu Phe Thr Gly Leu Ile Gly Gly Thr Asn Asn 
        195                 200                 205             


Arg Ala Pro Gly Val Pro Ala Arg Phe Ser Gly Ser Leu Ile Gly Asp 
    210                 215                 220                 


Lys Ala Ala Leu Thr Ile Thr Gly Ala Gln Thr Glu Asp Glu Ala Ile 
225                 230                 235                 240 


Tyr Phe Cys Ala Leu Trp Tyr Ser Asn His Leu Val Phe Gly Gly Gly 
                245                 250                 255     


Thr Lys Leu Thr Val Leu Gly Glu Pro Lys Ser Pro Asp Lys Thr His 
            260                 265                 270         


Thr Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser Val Phe 
        275                 280                 285             


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ala Arg Thr Pro 
    290                 295                 300                 


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
305                 310                 315                 320 


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
                325                 330                 335     


Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 
            340                 345                 350         


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
        355                 360                 365             


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
    370                 375                 380                 


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
385                 390                 395                 400 


Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
                405                 410                 415     


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
            420                 425                 430         


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
        435                 440                 445             


Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
    450                 455                 460                 


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
465                 470                 475                 480 


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Ile Ile 
                485                 490                 495     


Ser Phe Phe Leu Ala Leu Thr Ser Thr Ala Leu Leu Phe Leu Leu Phe 
            500                 505                 510         


Phe Leu Thr Leu Arg Phe Ser Val Val Lys Arg Gly Arg Lys Lys Leu 
        515                 520                 525             


Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln 
    530                 535                 540                 


Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly 
545                 550                 555                 560 


Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
                565                 570                 575     


Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
            580                 585                 590         


Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
        595                 600                 605             


Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu 
    610                 615                 620                 


Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys 
625                 630                 635                 640 


Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu 
                645                 650                 655     


Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu 
            660                 665                 670         


Pro Pro Arg 
        675 


<210>  27

<211>  457
<212>  PRT
<213>  artificial sequence

<220>
<223>  V1 humanized cmHsp70.1 scCAR polypeptide

<400>  27
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu 
            20                  25                  30          


Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe 
        35                  40                  45              


Ser Leu Ser Arg Asn Ser Val His Trp Val Arg Gln Ala Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Gly Gly Ser Thr Asp Tyr 
65                  70                  75                  80  


Asn Ser Ala Leu Lys Ser Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys 
                85                  90                  95      


Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Arg Asn Gly Gly Tyr Asp Val Phe His Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Ala Val Val Thr Gln Glu 
145                 150                 155                 160 


Pro Ser Leu Thr Val Ser Pro Gly Gly Thr Val Thr Leu Thr Cys Arg 
                165                 170                 175     


Ser Ser Thr Gly Ala Val Thr Thr Ser Asn Tyr Ala Asn Trp Val Gln 
            180                 185                 190         


Gln Lys Pro Gly Gln Ala Pro Arg Gly Leu Ile Gly Gly Thr Asn Asn 
        195                 200                 205             


Arg Ala Pro Trp Thr Pro Ala Arg Phe Ser Gly Ser Leu Leu Gly Gly 
    210                 215                 220                 


Lys Ala Ala Leu Thr Leu Ser Gly Val Gln Pro Glu Asp Glu Ala Glu 
225                 230                 235                 240 


Tyr Tyr Cys Ala Leu Trp Tyr Ser Asn His Leu Val Phe Gly Gly Gly 
                245                 250                 255     


Thr Lys Leu Thr Val Leu Gly Gly Leu Ala Val Ser Thr Ile Ser Ser 
            260                 265                 270         


Phe Phe Pro Pro Gly Tyr Gln Ile Tyr Ile Trp Ala Pro Leu Ala Gly 
        275                 280                 285             


Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys 
    290                 295                 300                 


Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg 
305                 310                 315                 320 


Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro 
                325                 330                 335     


Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser 
            340                 345                 350         


Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
        355                 360                 365             


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
    370                 375                 380                 


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
385                 390                 395                 400 


Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
                405                 410                 415     


Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
            420                 425                 430         


Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
        435                 440                 445             


Leu His Met Gln Ala Leu Pro Pro Arg 
    450                 455         


<210>  28

<211>  460
<212>  PRT
<213>  artificial sequence

<220>
<223>  V2 humanized cmHsp70.1 scCAR polypeptide

<400>  28
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu 
            20                  25                  30          


Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe 
        35                  40                  45              


Ser Leu Ser Arg Asn Ser Val His Trp Val Arg Gln Ala Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Gly Gly Ser Thr Asp Tyr 
65                  70                  75                  80  


Asn Ser Ala Leu Lys Ser Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys 
                85                  90                  95      


Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Arg Asn Gly Gly Tyr Asp Val Phe His Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Ala Val Val Thr Gln Glu 
145                 150                 155                 160 


Pro Ser Leu Thr Val Ser Pro Gly Gly Thr Val Thr Leu Thr Cys Arg 
                165                 170                 175     


Ser Ser Thr Gly Ala Val Thr Thr Ser Asn Tyr Ala Asn Trp Val Gln 
            180                 185                 190         


Gln Lys Pro Gly Gln Ala Pro Arg Gly Leu Ile Gly Gly Thr Asn Asn 
        195                 200                 205             


Arg Ala Pro Trp Thr Pro Ala Arg Phe Ser Gly Ser Leu Leu Gly Gly 
    210                 215                 220                 


Lys Ala Ala Leu Thr Leu Ser Gly Val Gln Pro Glu Asp Glu Ala Glu 
225                 230                 235                 240 


Tyr Tyr Cys Ala Leu Trp Tyr Ser Asn His Leu Val Phe Gly Gly Gly 
                245                 250                 255     


Thr Lys Leu Thr Val Leu Gly Gly Leu Ala Val Ser Thr Ile Ser Ser 
            260                 265                 270         


Phe Phe Pro Pro Gly Tyr Gln Ile Ile Ser Phe Phe Leu Ala Leu Thr 
        275                 280                 285             


Ser Thr Ala Leu Leu Phe Leu Leu Phe Phe Leu Thr Leu Arg Phe Ser 
    290                 295                 300                 


Val Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro 
305                 310                 315                 320 


Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys 
                325                 330                 335     


Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe 
            340                 345                 350         


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu 
        355                 360                 365             


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
    370                 375                 380                 


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys 
385                 390                 395                 400 


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
                405                 410                 415     


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
            420                 425                 430         


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
        435                 440                 445             


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
    450                 455                 460 


<210>  29

<211>  486
<212>  PRT
<213>  artificial sequence

<220>
<223>  V3 humanized cmHsp70.1 scCAR polypeptide

<400>  29
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu 
            20                  25                  30          


Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe 
        35                  40                  45              


Ser Leu Ser Arg Asn Ser Val His Trp Val Arg Gln Ala Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Gly Gly Ser Thr Asp Tyr 
65                  70                  75                  80  


Asn Ser Ala Leu Lys Ser Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys 
                85                  90                  95      


Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Arg Asn Gly Gly Tyr Asp Val Phe His Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Ala Val Val Thr Gln Glu 
145                 150                 155                 160 


Pro Ser Leu Thr Val Ser Pro Gly Gly Thr Val Thr Leu Thr Cys Arg 
                165                 170                 175     


Ser Ser Thr Gly Ala Val Thr Thr Ser Asn Tyr Ala Asn Trp Val Gln 
            180                 185                 190         


Gln Lys Pro Gly Gln Ala Pro Arg Gly Leu Ile Gly Gly Thr Asn Asn 
        195                 200                 205             


Arg Ala Pro Trp Thr Pro Ala Arg Phe Ser Gly Ser Leu Leu Gly Gly 
    210                 215                 220                 


Lys Ala Ala Leu Thr Leu Ser Gly Val Gln Pro Glu Asp Glu Ala Glu 
225                 230                 235                 240 


Tyr Tyr Cys Ala Leu Trp Tyr Ser Asn His Leu Val Phe Gly Gly Gly 
                245                 250                 255     


Thr Lys Leu Thr Val Leu Gly Thr Thr Thr Pro Ala Pro Arg Pro Pro 
            260                 265                 270         


Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu 
        275                 280                 285             


Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp 
    290                 295                 300                 


Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly 
305                 310                 315                 320 


Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg 
                325                 330                 335     


Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln 
            340                 345                 350         


Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu 
        355                 360                 365             


Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala 
    370                 375                 380                 


Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu 
385                 390                 395                 400 


Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp 
                405                 410                 415     


Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu 
            420                 425                 430         


Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile 
        435                 440                 445             


Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr 
    450                 455                 460                 


Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met 
465                 470                 475                 480 


Gln Ala Leu Pro Pro Arg 
                485     


<210>  30

<211>  489
<212>  PRT
<213>  artificial sequence

<220>
<223>  V4 humanized cmHsp70.1 scCAR polypeptide

<400>  30
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu 
            20                  25                  30          


Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe 
        35                  40                  45              


Ser Leu Ser Arg Asn Ser Val His Trp Val Arg Gln Ala Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Gly Gly Ser Thr Asp Tyr 
65                  70                  75                  80  


Asn Ser Ala Leu Lys Ser Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys 
                85                  90                  95      


Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Arg Asn Gly Gly Tyr Asp Val Phe His Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Ala Val Val Thr Gln Glu 
145                 150                 155                 160 


Pro Ser Leu Thr Val Ser Pro Gly Gly Thr Val Thr Leu Thr Cys Arg 
                165                 170                 175     


Ser Ser Thr Gly Ala Val Thr Thr Ser Asn Tyr Ala Asn Trp Val Gln 
            180                 185                 190         


Gln Lys Pro Gly Gln Ala Pro Arg Gly Leu Ile Gly Gly Thr Asn Asn 
        195                 200                 205             


Arg Ala Pro Trp Thr Pro Ala Arg Phe Ser Gly Ser Leu Leu Gly Gly 
    210                 215                 220                 


Lys Ala Ala Leu Thr Leu Ser Gly Val Gln Pro Glu Asp Glu Ala Glu 
225                 230                 235                 240 


Tyr Tyr Cys Ala Leu Trp Tyr Ser Asn His Leu Val Phe Gly Gly Gly 
                245                 250                 255     


Thr Lys Leu Thr Val Leu Gly Thr Thr Thr Pro Ala Pro Arg Pro Pro 
            260                 265                 270         


Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu 
        275                 280                 285             


Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp 
    290                 295                 300                 


Phe Ala Cys Asp Ile Ile Ser Phe Phe Leu Ala Leu Thr Ser Thr Ala 
305                 310                 315                 320 


Leu Leu Phe Leu Leu Phe Phe Leu Thr Leu Arg Phe Ser Val Val Lys 
                325                 330                 335     


Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg 
            340                 345                 350         


Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro 
        355                 360                 365             


Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser 
    370                 375                 380                 


Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
385                 390                 395                 400 


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
                405                 410                 415     


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
            420                 425                 430         


Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
        435                 440                 445             


Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
    450                 455                 460                 


Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
465                 470                 475                 480 


Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 


<210>  31

<211>  672
<212>  PRT
<213>  artificial sequence

<220>
<223>  V5 humanized cmHsp70.1 scCAR polypeptide

<400>  31
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu 
            20                  25                  30          


Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe 
        35                  40                  45              


Ser Leu Ser Arg Asn Ser Val His Trp Val Arg Gln Ala Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Gly Gly Ser Thr Asp Tyr 
65                  70                  75                  80  


Asn Ser Ala Leu Lys Ser Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys 
                85                  90                  95      


Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Arg Asn Gly Gly Tyr Asp Val Phe His Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Ala Val Val Thr Gln Glu 
145                 150                 155                 160 


Pro Ser Leu Thr Val Ser Pro Gly Gly Thr Val Thr Leu Thr Cys Arg 
                165                 170                 175     


Ser Ser Thr Gly Ala Val Thr Thr Ser Asn Tyr Ala Asn Trp Val Gln 
            180                 185                 190         


Gln Lys Pro Gly Gln Ala Pro Arg Gly Leu Ile Gly Gly Thr Asn Asn 
        195                 200                 205             


Arg Ala Pro Trp Thr Pro Ala Arg Phe Ser Gly Ser Leu Leu Gly Gly 
    210                 215                 220                 


Lys Ala Ala Leu Thr Leu Ser Gly Val Gln Pro Glu Asp Glu Ala Glu 
225                 230                 235                 240 


Tyr Tyr Cys Ala Leu Trp Tyr Ser Asn His Leu Val Phe Gly Gly Gly 
                245                 250                 255     


Thr Lys Leu Thr Val Leu Gly Glu Pro Lys Ser Pro Asp Lys Thr His 
            260                 265                 270         


Thr Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser Val Phe 
        275                 280                 285             


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ala Arg Thr Pro 
    290                 295                 300                 


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
305                 310                 315                 320 


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
                325                 330                 335     


Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 
            340                 345                 350         


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
        355                 360                 365             


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
    370                 375                 380                 


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
385                 390                 395                 400 


Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
                405                 410                 415     


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
            420                 425                 430         


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
        435                 440                 445             


Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
    450                 455                 460                 


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
465                 470                 475                 480 


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Ile Tyr 
                485                 490                 495     


Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu 
            500                 505                 510         


Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile 
        515                 520                 525             


Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp 
    530                 535                 540                 


Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
545                 550                 555                 560 


Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
                565                 570                 575     


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            580                 585                 590         


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        595                 600                 605             


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    610                 615                 620                 


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
625                 630                 635                 640 


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                645                 650                 655     


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            660                 665                 670         


<210>  32

<211>  675
<212>  PRT
<213>  artificial sequence

<220>
<223>  V6 humanized cmHsp70.1 scCAR polypeptide

<400>  32
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu 
            20                  25                  30          


Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe 
        35                  40                  45              


Ser Leu Ser Arg Asn Ser Val His Trp Val Arg Gln Ala Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Gly Gly Ser Thr Asp Tyr 
65                  70                  75                  80  


Asn Ser Ala Leu Lys Ser Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys 
                85                  90                  95      


Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Arg Asn Gly Gly Tyr Asp Val Phe His Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Ala Val Val Thr Gln Glu 
145                 150                 155                 160 


Pro Ser Leu Thr Val Ser Pro Gly Gly Thr Val Thr Leu Thr Cys Arg 
                165                 170                 175     


Ser Ser Thr Gly Ala Val Thr Thr Ser Asn Tyr Ala Asn Trp Val Gln 
            180                 185                 190         


Gln Lys Pro Gly Gln Ala Pro Arg Gly Leu Ile Gly Gly Thr Asn Asn 
        195                 200                 205             


Arg Ala Pro Trp Thr Pro Ala Arg Phe Ser Gly Ser Leu Leu Gly Gly 
    210                 215                 220                 


Lys Ala Ala Leu Thr Leu Ser Gly Val Gln Pro Glu Asp Glu Ala Glu 
225                 230                 235                 240 


Tyr Tyr Cys Ala Leu Trp Tyr Ser Asn His Leu Val Phe Gly Gly Gly 
                245                 250                 255     


Thr Lys Leu Thr Val Leu Gly Glu Pro Lys Ser Pro Asp Lys Thr His 
            260                 265                 270         


Thr Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser Val Phe 
        275                 280                 285             


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ala Arg Thr Pro 
    290                 295                 300                 


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
305                 310                 315                 320 


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
                325                 330                 335     


Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 
            340                 345                 350         


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
        355                 360                 365             


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
    370                 375                 380                 


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
385                 390                 395                 400 


Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
                405                 410                 415     


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
            420                 425                 430         


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
        435                 440                 445             


Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
    450                 455                 460                 


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
465                 470                 475                 480 


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Ile Ile 
                485                 490                 495     


Ser Phe Phe Leu Ala Leu Thr Ser Thr Ala Leu Leu Phe Leu Leu Phe 
            500                 505                 510         


Phe Leu Thr Leu Arg Phe Ser Val Val Lys Arg Gly Arg Lys Lys Leu 
        515                 520                 525             


Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln 
    530                 535                 540                 


Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly 
545                 550                 555                 560 


Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
                565                 570                 575     


Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
            580                 585                 590         


Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
        595                 600                 605             


Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu 
    610                 615                 620                 


Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys 
625                 630                 635                 640 


Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu 
                645                 650                 655     


Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu 
            660                 665                 670         


Pro Pro Arg 
        675 


<210>  33

<211>  10
<212>  PRT
<213>  artificial sequence

<220>
<223>  CD20 mimotope

<400>  33
Cys Pro Tyr Ser Asn Pro Ser Leu Cys Ser 
1               5                   10  


<210>  34

<211>  24
<212>  PRT
<213>  artificial sequence

<220>
<223>  Epitope for palivizumab

<400>  34
Asn Ser Glu Leu Leu Ser Leu Ile Asn Asp Met Pro Ile Thr Asn Asp 
1               5                   10                  15      


Gln Lys Lys Leu Met Ser Asn Asn 
            20                  


<210>  35

<211>  12
<212>  PRT
<213>  artificial sequence

<220>
<223>  Mimotope 1 for cetuximab

<400>  35
Cys Gln Phe Asp Leu Ser Thr Arg Arg Leu Lys Cys 
1               5                   10          


<210>  36

<211>  12
<212>  PRT
<213>  artificial sequence

<220>
<223>  Mimotope 2 for cetuximab

<400>  36
Cys Gln Tyr Asn Leu Ser Ser Arg Ala Leu Lys Cys 
1               5                   10          


<210>  37

<211>  12
<212>  PRT
<213>  artificial sequence

<220>
<223>  Mimotope 3 for cetuximab

<400>  37
Cys Val Trp Gln Arg Trp Gln Lys Ser Tyr Val Cys 
1               5                   10          


<210>  38

<211>  12
<212>  PRT
<213>  artificial sequence

<220>
<223>  Mimotope 4 for cetuximab

<400>  38
Cys Met Trp Asp Arg Phe Ser Arg Trp Tyr Lys Cys 
1               5                   10          


<210>  39

<211>  25
<212>  PRT
<213>  artificial sequence

<220>
<223>  Epitope 1 for nivolumab

<400>  39
Ser Phe Val Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp 
1               5                   10                  15      


Lys Leu Ala Ala Phe Pro Glu Asp Arg 
            20                  25  


<210>  40

<211>  19
<212>  PRT
<213>  artificial sequence

<220>
<223>  Epitope 2 for nivolumab

<400>  40
Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu Ala Pro Lys Ala Gln 
1               5                   10                  15      


Ile Lys Glu 
            


<210>  41

<211>  14
<212>  PRT
<213>  artificial sequence

<220>
<223>  Epitope 1 for CD34

<400>  41
Thr Phe Ser Asn Val Ser Thr Asn Val Ser Tyr Gln Glu Thr 
1               5                   10                  


<210>  42

<211>  14
<212>  PRT
<213>  artificial sequence

<220>
<223>  Epitope 2 for CD34

<400>  42
Asn Thr Asn Ser Ser Val Gln Ser Gln Thr Ser Val Ile Ser 
1               5                   10                  



<210>  43

<211>  49
<212>  PRT
<213>  artificial sequence

<220>
<223>  Target TALEN TRAC_T01

<400>  43
Thr Thr Gly Thr Cys Cys Cys Ala Cys Ala Gly Ala Thr Ala Thr Cys 
1               5                   10                  15      


Cys Ala Gly Ala Ala Cys Cys Cys Thr Gly Ala Cys Cys Cys Thr Gly 
            20                  25                  30          


Cys Cys Gly Thr Gly Thr Ala Cys Cys Ala Gly Cys Thr Gly Ala Gly 
        35                  40                  45              


Ala 
    



<210>  44

<211>  530
<212>  PRT
<213>  artificial sequence

<220>
<223>  TAL binding domain  TRAC_T01-L

<400>  44
Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys 
1               5                   10                  15      


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala 
            20                  25                  30          


His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly 
        35                  40                  45              


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
    50                  55                  60                  


Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn 
65                  70                  75                  80  


Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
                85                  90                  95      


Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala 
            100                 105                 110         


Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
        115                 120                 125             


Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala 
    130                 135                 140                 


Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
145                 150                 155                 160 


Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val 
                165                 170                 175     


Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val 
            180                 185                 190         


Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu 
        195                 200                 205             


Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu 
    210                 215                 220                 


Thr Val Gln Ala Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr 
225                 230                 235                 240 


Pro Glu Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala 
                245                 250                 255     


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly 
            260                 265                 270         


Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys 
        275                 280                 285             


Gln Ala Leu Glu Thr Val Gln Ala Leu Leu Pro Val Leu Cys Gln Ala 
    290                 295                 300                 


His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly 
305                 310                 315                 320 


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
                325                 330                 335     


Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn 
            340                 345                 350         


Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Ala Leu Leu Pro Val 
        355                 360                 365             


Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala 
    370                 375                 380                 


Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
385                 390                 395                 400 


Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala 
                405                 410                 415     


Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Ala 
            420                 425                 430         


Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val 
        435                 440                 445             


Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val 
    450                 455                 460                 


Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu 
465                 470                 475                 480 


Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu 
                485                 490                 495     


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr 
            500                 505                 510         


Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Arg Pro Ala 
        515                 520                 525             


Leu Glu 
    530 


<210>  45

<211>  530
<212>  PRT
<213>  artificial sequence

<220>
<223>  TAL binding domain TRAC_T01-R

<400>  45
Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys 
1               5                   10                  15      


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala 
            20                  25                  30          


His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly 
        35                  40                  45              


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
    50                  55                  60                  


Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser His 
65                  70                  75                  80  


Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
                85                  90                  95      


Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala 
            100                 105                 110         


Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Ala Leu Leu 
        115                 120                 125             


Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala 
    130                 135                 140                 


Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
145                 150                 155                 160 


Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val 
                165                 170                 175     


Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val 
            180                 185                 190         


Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln 
        195                 200                 205             


Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu 
    210                 215                 220                 


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr 
225                 230                 235                 240 


Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala 
                245                 250                 255     


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly 
            260                 265                 270         


Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys 
        275                 280                 285             


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala 
    290                 295                 300                 


His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly 
305                 310                 315                 320 


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
                325                 330                 335     


Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn 
            340                 345                 350         


Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Ala Leu Leu Pro Val 
        355                 360                 365             


Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala 
    370                 375                 380                 


Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
385                 390                 395                 400 


Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala 
                405                 410                 415     


Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Ala 
            420                 425                 430         


Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val 
        435                 440                 445             


Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val 
    450                 455                 460                 


Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln 
465                 470                 475                 480 


Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu 
                485                 490                 495     


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr 
            500                 505                 510         


Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Arg Pro Ala 
        515                 520                 525             


Leu Glu 
    530 



<210>  46

<211>  2814
<212>  DNA
<213>  artificial sequence

<220>
<223>  polynucleotide encoding  TRAC_T01-L TALEN

<400>  46
atgggcgatc ctaaaaagaa acgtaaggtc atcgattacc catacgatgt tccagattac     60
gctatcgata tcgccgatct acgcacgctc ggctacagcc agcagcaaca ggagaagatc    120
aaaccgaagg ttcgttcgac agtggcgcag caccacgagg cactggtcgg ccacgggttt    180
acacacgcgc acatcgttgc gttaagccaa cacccggcag cgttagggac cgtcgctgtc    240
aagtatcagg acatgatcgc agcgttgcca gaggcgacac acgaagcgat cgttggcgtc    300
ggcaaacagt ggtccggcgc acgcgctctg gaggccttgc tcacggtggc gggagagttg    360
agaggtccac cgttacagtt ggacacaggc caacttctca agattgcaaa acgtggcggc    420
gtgaccgcag tggaggcagt gcatgcatgg cgcaatgcac tgacgggtgc cccgctcaac    480
ttgacccccc agcaggtggt ggccatcgcc agcaatggcg gtggcaagca ggcgctggag    540
acggtccagc ggctgttgcc ggtgctgtgc caggcccacg gcttgacccc ccagcaggtg    600
gtggccatcg ccagcaataa tggtggcaag caggcgctgg agacggtcca gcggctgttg    660
ccggtgctgt gccaggccca cggcttgacc ccccagcagg tggtggccat cgccagcaat    720
ggcggtggca agcaggcgct ggagacggtc cagcggctgt tgccggtgct gtgccaggcc    780
cacggcttga ccccggagca ggtggtggcc atcgccagcc acgatggcgg caagcaggcg    840
ctggagacgg tccagcggct gttgccggtg ctgtgccagg cccacggctt gaccccggag    900
caggtggtgg ccatcgccag ccacgatggc ggcaagcagg cgctggagac ggtccagcgg    960
ctgttgccgg tgctgtgcca ggcccacggc ttgaccccgg agcaggtggt ggccatcgcc   1020
agccacgatg gcggcaagca ggcgctggag acggtccagc ggctgttgcc ggtgctgtgc   1080
caggcccacg gcttgacccc ggagcaggtg gtggccatcg ccagcaatat tggtggcaag   1140
caggcgctgg agacggtgca ggcgctgttg ccggtgctgt gccaggccca cggcttgacc   1200
ccggagcagg tggtggccat cgccagccac gatggcggca agcaggcgct ggagacggtc   1260
cagcggctgt tgccggtgct gtgccaggcc cacggcttga ccccggagca ggtggtggcc   1320
atcgccagca atattggtgg caagcaggcg ctggagacgg tgcaggcgct gttgccggtg   1380
ctgtgccagg cccacggctt gaccccccag caggtggtgg ccatcgccag caataatggt   1440
ggcaagcagg cgctggagac ggtccagcgg ctgttgccgg tgctgtgcca ggcccacggc   1500
ttgaccccgg agcaggtggt ggccatcgcc agcaatattg gtggcaagca ggcgctggag   1560
acggtgcagg cgctgttgcc ggtgctgtgc caggcccacg gcttgacccc ccagcaggtg   1620
gtggccatcg ccagcaatgg cggtggcaag caggcgctgg agacggtcca gcggctgttg   1680
ccggtgctgt gccaggccca cggcttgacc ccggagcagg tggtggccat cgccagcaat   1740
attggtggca agcaggcgct ggagacggtg caggcgctgt tgccggtgct gtgccaggcc   1800
cacggcttga ccccccagca ggtggtggcc atcgccagca atggcggtgg caagcaggcg   1860
ctggagacgg tccagcggct gttgccggtg ctgtgccagg cccacggctt gaccccggag   1920
caggtggtgg ccatcgccag ccacgatggc ggcaagcagg cgctggagac ggtccagcgg   1980
ctgttgccgg tgctgtgcca ggcccacggc ttgacccctc agcaggtggt ggccatcgcc   2040
agcaatggcg gcggcaggcc ggcgctggag agcattgttg cccagttatc tcgccctgat   2100
ccggcgttgg ccgcgttgac caacgaccac ctcgtcgcct tggcctgcct cggcgggcgt   2160
cctgcgctgg atgcagtgaa aaagggattg ggggatccta tcagccgttc ccagctggtg   2220
aagtccgagc tggaggagaa gaaatccgag ttgaggcaca agctgaagta cgtgccccac   2280
gagtacatcg agctgatcga gatcgcccgg aacagcaccc aggaccgtat cctggagatg   2340
aaggtgatgg agttcttcat gaaggtgtac ggctacaggg gcaagcacct gggcggctcc   2400
aggaagcccg acggcgccat ctacaccgtg ggctccccca tcgactacgg cgtgatcgtg   2460
gacaccaagg cctactccgg cggctacaac ctgcccatcg gccaggccga cgaaatgcag   2520
aggtacgtgg aggagaacca gaccaggaac aagcacatca accccaacga gtggtggaag   2580
gtgtacccct ccagcgtgac cgagttcaag ttcctgttcg tgtccggcca cttcaagggc   2640
aactacaagg cccagctgac caggctgaac cacatcacca actgcaacgg cgccgtgctg   2700
tccgtggagg agctcctgat cggcggcgag atgatcaagg ccggcaccct gaccctggag   2760
gaggtgagga ggaagttcaa caacggcgag atcaacttcg cggccgactg ataa         2814


<210>  47

<211>  2832
<212>  DNA
<213>  artificial sequence

<220>
<223>  polynucleotide encoding TRAC_T01-R TALEN

<400>  47
atgggcgatc ctaaaaagaa acgtaaggtc atcgataagg agaccgccgc tgccaagttc     60
gagagacagc acatggacag catcgatatc gccgatctac gcacgctcgg ctacagccag    120
cagcaacagg agaagatcaa accgaaggtt cgttcgacag tggcgcagca ccacgaggca    180
ctggtcggcc acgggtttac acacgcgcac atcgttgcgt taagccaaca cccggcagcg    240
ttagggaccg tcgctgtcaa gtatcaggac atgatcgcag cgttgccaga ggcgacacac    300
gaagcgatcg ttggcgtcgg caaacagtgg tccggcgcac gcgctctgga ggccttgctc    360
acggtggcgg gagagttgag aggtccaccg ttacagttgg acacaggcca acttctcaag    420
attgcaaaac gtggcggcgt gaccgcagtg gaggcagtgc atgcatggcg caatgcactg    480
acgggtgccc cgctcaactt gaccccggag caggtggtgg ccatcgccag ccacgatggc    540
ggcaagcagg cgctggagac ggtccagcgg ctgttgccgg tgctgtgcca ggcccacggc    600
ttgacccccc agcaggtggt ggccatcgcc agcaatggcg gtggcaagca ggcgctggag    660
acggtccagc ggctgttgcc ggtgctgtgc caggcccacg gcttgacccc ggagcaggtg    720
gtggccatcg ccagccacga tggcggcaag caggcgctgg agacggtcca gcggctgttg    780
ccggtgctgt gccaggccca cggcttgacc ccggagcagg tggtggccat cgccagcaat    840
attggtggca agcaggcgct ggagacggtg caggcgctgt tgccggtgct gtgccaggcc    900
cacggcttga ccccccagca ggtggtggcc atcgccagca ataatggtgg caagcaggcg    960
ctggagacgg tccagcggct gttgccggtg ctgtgccagg cccacggctt gaccccggag   1020
caggtggtgg ccatcgccag ccacgatggc ggcaagcagg cgctggagac ggtccagcgg   1080
ctgttgccgg tgctgtgcca ggcccacggc ttgacccccc agcaggtggt ggccatcgcc   1140
agcaatggcg gtggcaagca ggcgctggag acggtccagc ggctgttgcc ggtgctgtgc   1200
caggcccacg gcttgacccc ccagcaggtg gtggccatcg ccagcaataa tggtggcaag   1260
caggcgctgg agacggtcca gcggctgttg ccggtgctgt gccaggccca cggcttgacc   1320
ccccagcagg tggtggccat cgccagcaat aatggtggca agcaggcgct ggagacggtc   1380
cagcggctgt tgccggtgct gtgccaggcc cacggcttga ccccccagca ggtggtggcc   1440
atcgccagca atggcggtgg caagcaggcg ctggagacgg tccagcggct gttgccggtg   1500
ctgtgccagg cccacggctt gaccccggag caggtggtgg ccatcgccag caatattggt   1560
ggcaagcagg cgctggagac ggtgcaggcg ctgttgccgg tgctgtgcca ggcccacggc   1620
ttgaccccgg agcaggtggt ggccatcgcc agccacgatg gcggcaagca ggcgctggag   1680
acggtccagc ggctgttgcc ggtgctgtgc caggcccacg gcttgacccc ggagcaggtg   1740
gtggccatcg ccagcaatat tggtggcaag caggcgctgg agacggtgca ggcgctgttg   1800
ccggtgctgt gccaggccca cggcttgacc ccggagcagg tggtggccat cgccagccac   1860
gatggcggca agcaggcgct ggagacggtc cagcggctgt tgccggtgct gtgccaggcc   1920
cacggcttga ccccccagca ggtggtggcc atcgccagca ataatggtgg caagcaggcg   1980
ctggagacgg tccagcggct gttgccggtg ctgtgccagg cccacggctt gacccctcag   2040
caggtggtgg ccatcgccag caatggcggc ggcaggccgg cgctggagag cattgttgcc   2100
cagttatctc gccctgatcc ggcgttggcc gcgttgacca acgaccacct cgtcgccttg   2160
gcctgcctcg gcgggcgtcc tgcgctggat gcagtgaaaa agggattggg ggatcctatc   2220
agccgttccc agctggtgaa gtccgagctg gaggagaaga aatccgagtt gaggcacaag   2280
ctgaagtacg tgccccacga gtacatcgag ctgatcgaga tcgcccggaa cagcacccag   2340
gaccgtatcc tggagatgaa ggtgatggag ttcttcatga aggtgtacgg ctacaggggc   2400
aagcacctgg gcggctccag gaagcccgac ggcgccatct acaccgtggg ctcccccatc   2460
gactacggcg tgatcgtgga caccaaggcc tactccggcg gctacaacct gcccatcggc   2520
caggccgacg aaatgcagag gtacgtggag gagaaccaga ccaggaacaa gcacatcaac   2580
cccaacgagt ggtggaaggt gtacccctcc agcgtgaccg agttcaagtt cctgttcgtg   2640
tccggccact tcaagggcaa ctacaaggcc cagctgacca ggctgaacca catcaccaac   2700
tgcaacggcg ccgtgctgtc cgtggaggag ctcctgatcg gcggcgagat gatcaaggcc   2760
ggcaccctga ccctggagga ggtgaggagg aagttcaaca acggcgagat caacttcgcg   2820
gccgactgat aa                                                       2832




