                         SEQUENCE LISTING

<110>  The Trustees Of The University Of Pennsylvania
       Weiner, David
       Reuschel, Emma L
       Muthumani, Karuppiah
 
<120>  IMMUNOGENICITY OF AN OPTIMIZED SYNTHETIC CONSENSUS DNA VACCINE 
       FOR PORCINE EPIDEMIC DIARRHEA VIRUS

<130>  206108-0056-00-WO.604992

<150>  US 62/107,165
<151>  2015-01-23

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  4119
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PEDV-S 64 consensus

<400>  1
ctgcctcagg atgtcacaag atgctctgct aataccaact tcaggagatt ctttagcaag       60

tttaatgtgc aggcaccagc tgtggtcgtg ctcggcggat acctgcctat cggggagaat      120

cagggcggcg tgaactccac ttggtattgc gctggccagc accctaccgc atctggagtg      180

catgggatct tcctgtcaca cattcgggga gggcatgggt tcgagatcgg cattagccag      240

gagccatttg acccttccgg gtaccagctg tatctccaca aggccaccaa cggcaataca      300

aacgcaactg cccgactgag gatctgccag ttccctagca ttaagaccct ggggcccaca      360

gccaacaatg acgtgaccac aggccgcaat tgtctgttta acaaagctat tcccgcacac      420

atgtccgatg gcgagcattc tgtcgtggga atcacttggg acaacgatag ggtcaccgtg      480

ttctccgaca agatctacta tttctacttt aaaaacgatt ggagcagagt ggccaccaag      540

tgctataatt ccggcggatg tgctatgcag tacgtctatg agcccacata ctatatgctg      600

aacgtgactt ctgccggcga agacggaatt tcataccagc catgcaccgc caattgtatc      660

ggctatgccg ctaacgtgtt cgctacagag cccaatggcc acatcccaga aggattcagc      720

tttaacaatt ggtttctgct ctctaacgat tcaaccctgg tgcatggcaa ggtcgtgtcc      780

aatcagcccc tgctcgtgaa ctgcctgctc gccatcccaa aaatctacgg actggggcag      840

ttctttagct tcaaccagac catcgacgga gtctgtaatg gagcagcagt gcagcgagca      900

ccagaggctc tgaggttcaa tatcaacgat acaagtgtca ttctggctga aggaagcatc      960

gtgctccaca ctgcactggg gaccaacctc tcattcgtgt gcagtaatag ctccgaccct     1020

catctggcaa catttgccat cccactcggc gccactcagg tcccttacta ttgtttcctg     1080

aaggtggata catacaactc cactgtctat aaatttctgg ccgtgctgcc cccaaccgtc     1140

agggagatcg tgattacaaa gtacggcgac gtgtacgtga acggcttcgg atacctgcac     1200

ctcggactgc tcgatgccgt gaccatcaac ttcaccgggc atggcactga cgatgacgtg     1260

tccggcttct ggaccattgc atctacaaac tttgtcgacg ccctgatcga ggtgcaggga     1320

accgccatcc agagaattct gtactgcgat gaccctgtct cacagctcaa atgtagtcag     1380

gtggctttcg acctggatga cggcttttat cccatttctt cacggaacct gctcagtcac     1440

gagcagccca tcagcttcgt gaccctgcca agcttcaatg atcactcctt tgtcaacatc     1500

acagtgtcag ccagttttgg gggccattca ggagccaacc tgattgctag tgacactacc     1560

atcaatgggt tcagtagctt ttgcgtggat actagacagt tcaccatcag tctgttttac     1620

aatgtcacca actcatacgg ctatgtgagc aagtcccagg acagcaactg tcccttcaca     1680

ctgcagtccg tcaacgatta cctctctttc tcaaagttct gcgtgagtac cagcctgctc     1740

gcttctgcat gtacaatcga cctgttcggg tacccagagt ttggctcctc ttcaggaggg     1800

ggcgtgaagt tcacttccct gtatttccag tttaccaagg gagagctgat caccgggaca     1860

cctaaacccc tcgaaggggt caccgacgtg agcttcatga cactggacgt gtgcaccaag     1920

tacacaatct acggcttcaa aggagagggg atcattactc tgaccaacag tagctttctc     1980

gccggcgtgt actatacctc cgactctgga cagctgctcg ccttcaagaa tgtgacttct     2040

ggcgctgtct actcagtgac cccatgtagc ttcagcgagc aggctgcata tgtggatgac     2100

gatatcgtcg gagtgatttc ctctctgtca agtagcacat tcaacagcac tagggagctg     2160

ccagggttct tttaccacag caatgacggc tccaactgca ccgaacctgt cctggtgtac     2220

tccaacattg gcgtctgtaa gagcgggtcc atcggctatg tgccttctca gtcaggacag     2280

gtcaaaatcg cccctacagt gactgggaat atcagcattc ccacaaactt cagtatgagc     2340

attagaactg agtacctgca gctctataac acccccgtct ctgtggactg cgccacatac     2400

gtgtgcaatg gcaacagccg atgcaagcag ctgctcactc agtataccgc cgcttgtaaa     2460

accatcgaaa gtgctctgca gctcagcgca aggctggagt ccgtcgaagt gaactctatg     2520

ctgacaattt cagaggaagc actgcagctc gccactatct cctctttcaa tggcgacgga     2580

tacaacttca ccaacgtgct gggcgtctcc gtgtatgatc ccgcctctgg aagagtcgtg     2640

cagaagcggt ccttcatcga ggacctgctg ttcaacaagg tcgtgaccaa cgggctgggc     2700

acagtggacg aagattacaa gaggtgctct aacggccgat cagtcgcaga cctggtgtgc     2760

gctcagtact atagcggggt catggtgctg ccaggagtcg tggatgcaga gaaactgcac     2820

atgtactccg cctctctgat cggagggatg gtgctcggcg gattcaccgc agccgctctg     2880

cctttttcct acgctgtgca ggcaagactg aactatctgg ccctccagac agacgtgctg     2940

cagcggaatc agcagctgct cgccgagagt ttcaatagcg ccatcggaaa cattaccagt     3000

gcttttgaga gcgtgaagga agcaatctcc cagacttcta aagggctgaa caccgtcgcc     3060

cacgctctca caaaggtgca ggaagtcgtg aatagccagg gagcagccct gacacagctc     3120

actgtgcagc tgcagcacaa cttccaggcc atctcaagta gcatcgacga tatctacagc     3180

aggctggaca ttctcagtgc tgacgtgcag gtggataggc tgatcaccgg cagactgtcc     3240

gccctcaacg ctttcgtcgc acagaccctg acaaagtaca cagaggtgca ggcatctcgc     3300

aagctggccc agcagaaagt caacgaatgc gtgaaatcac agagtcagcg atacggattc     3360

tgtgggggcg acggggagca catcttttcc ctggtgcagg ctgcacctca gggactgctc     3420

ttcctgcata ccgtcctcgt gcccggcgac tttgtcgatg tgatcgcaat tgccggactg     3480

tgcgtgaacg atgagatcgc cctgaccctc cgagaaccag gactggtgct cttcacacac     3540

gagctgcaga atcatactgc taccgaatac ttcgtgtcct ctcggcgcat gtttgagccc     3600

cggaagccaa ccgtcagcga cttcgtgcag atcgaatcct gcgtcgtgac ttacgtgaac     3660

ctgacccgcg accagctccc agatgtcatc cctgactata ttgatgtgaa caaaactctg     3720

gatgagatcc tggcctctct ccccaatcga accggaccta gcctgccact cgacgtgttc     3780

aatgcaacat acctgaacct cactggggaa atcgctgatc tggagcagcg gagcgaatcc     3840

ctccgcaaca caactgagga actgcagtcc ctcatctaca atattaacaa tacactggtc     3900

gacctggagt ggctgaaccg agtggaaact tatatcaagt ggccatggtg ggtctggctg     3960

atcattttca tcgtgctcat ttttgtcgtg tccctgctcg tgttctgctg tatttctacc     4020

ggatgctgtg ggtgctgtgg ctgctgtgga gcctgctttt caggatgttg tcgggggcca     4080

agactccagc catacgaatt tgaaaaggtc cacgtccag                            4119


<210>  2
<211>  1373
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PEDV-S 64 consensus

<400>  2

Leu Pro Gln Asp Val Thr Arg Cys Ser Ala Asn Thr Asn Phe Arg Arg 
1               5                   10                  15      


Phe Phe Ser Lys Phe Asn Val Gln Ala Pro Ala Val Val Val Leu Gly 
            20                  25                  30          


Gly Tyr Leu Pro Ile Gly Glu Asn Gln Gly Gly Val Asn Ser Thr Trp 
        35                  40                  45              


Tyr Cys Ala Gly Gln His Pro Thr Ala Ser Gly Val His Gly Ile Phe 
    50                  55                  60                  


Leu Ser His Ile Arg Gly Gly His Gly Phe Glu Ile Gly Ile Ser Gln 
65                  70                  75                  80  


Glu Pro Phe Asp Pro Ser Gly Tyr Gln Leu Tyr Leu His Lys Ala Thr 
                85                  90                  95      


Asn Gly Asn Thr Asn Ala Thr Ala Arg Leu Arg Ile Cys Gln Phe Pro 
            100                 105                 110         


Ser Ile Lys Thr Leu Gly Pro Thr Ala Asn Asn Asp Val Thr Thr Gly 
        115                 120                 125             


Arg Asn Cys Leu Phe Asn Lys Ala Ile Pro Ala His Met Ser Asp Gly 
    130                 135                 140                 


Glu His Ser Val Val Gly Ile Thr Trp Asp Asn Asp Arg Val Thr Val 
145                 150                 155                 160 


Phe Ser Asp Lys Ile Tyr Tyr Phe Tyr Phe Lys Asn Asp Trp Ser Arg 
                165                 170                 175     


Val Ala Thr Lys Cys Tyr Asn Ser Gly Gly Cys Ala Met Gln Tyr Val 
            180                 185                 190         


Tyr Glu Pro Thr Tyr Tyr Met Leu Asn Val Thr Ser Ala Gly Glu Asp 
        195                 200                 205             


Gly Ile Ser Tyr Gln Pro Cys Thr Ala Asn Cys Ile Gly Tyr Ala Ala 
    210                 215                 220                 


Asn Val Phe Ala Thr Glu Pro Asn Gly His Ile Pro Glu Gly Phe Ser 
225                 230                 235                 240 


Phe Asn Asn Trp Phe Leu Leu Ser Asn Asp Ser Thr Leu Val His Gly 
                245                 250                 255     


Lys Val Val Ser Asn Gln Pro Leu Leu Val Asn Cys Leu Leu Ala Ile 
            260                 265                 270         


Pro Lys Ile Tyr Gly Leu Gly Gln Phe Phe Ser Phe Asn Gln Thr Ile 
        275                 280                 285             


Asp Gly Val Cys Asn Gly Ala Ala Val Gln Arg Ala Pro Glu Ala Leu 
    290                 295                 300                 


Arg Phe Asn Ile Asn Asp Thr Ser Val Ile Leu Ala Glu Gly Ser Ile 
305                 310                 315                 320 


Val Leu His Thr Ala Leu Gly Thr Asn Leu Ser Phe Val Cys Ser Asn 
                325                 330                 335     


Ser Ser Asp Pro His Leu Ala Thr Phe Ala Ile Pro Leu Gly Ala Thr 
            340                 345                 350         


Gln Val Pro Tyr Tyr Cys Phe Leu Lys Val Asp Thr Tyr Asn Ser Thr 
        355                 360                 365             


Val Tyr Lys Phe Leu Ala Val Leu Pro Pro Thr Val Arg Glu Ile Val 
    370                 375                 380                 


Ile Thr Lys Tyr Gly Asp Val Tyr Val Asn Gly Phe Gly Tyr Leu His 
385                 390                 395                 400 


Leu Gly Leu Leu Asp Ala Val Thr Ile Asn Phe Thr Gly His Gly Thr 
                405                 410                 415     


Asp Asp Asp Val Ser Gly Phe Trp Thr Ile Ala Ser Thr Asn Phe Val 
            420                 425                 430         


Asp Ala Leu Ile Glu Val Gln Gly Thr Ala Ile Gln Arg Ile Leu Tyr 
        435                 440                 445             


Cys Asp Asp Pro Val Ser Gln Leu Lys Cys Ser Gln Val Ala Phe Asp 
    450                 455                 460                 


Leu Asp Asp Gly Phe Tyr Pro Ile Ser Ser Arg Asn Leu Leu Ser His 
465                 470                 475                 480 


Glu Gln Pro Ile Ser Phe Val Thr Leu Pro Ser Phe Asn Asp His Ser 
                485                 490                 495     


Phe Val Asn Ile Thr Val Ser Ala Ser Phe Gly Gly His Ser Gly Ala 
            500                 505                 510         


Asn Leu Ile Ala Ser Asp Thr Thr Ile Asn Gly Phe Ser Ser Phe Cys 
        515                 520                 525             


Val Asp Thr Arg Gln Phe Thr Ile Ser Leu Phe Tyr Asn Val Thr Asn 
    530                 535                 540                 


Ser Tyr Gly Tyr Val Ser Lys Ser Gln Asp Ser Asn Cys Pro Phe Thr 
545                 550                 555                 560 


Leu Gln Ser Val Asn Asp Tyr Leu Ser Phe Ser Lys Phe Cys Val Ser 
                565                 570                 575     


Thr Ser Leu Leu Ala Ser Ala Cys Thr Ile Asp Leu Phe Gly Tyr Pro 
            580                 585                 590         


Glu Phe Gly Ser Ser Ser Gly Gly Gly Val Lys Phe Thr Ser Leu Tyr 
        595                 600                 605             


Phe Gln Phe Thr Lys Gly Glu Leu Ile Thr Gly Thr Pro Lys Pro Leu 
    610                 615                 620                 


Glu Gly Val Thr Asp Val Ser Phe Met Thr Leu Asp Val Cys Thr Lys 
625                 630                 635                 640 


Tyr Thr Ile Tyr Gly Phe Lys Gly Glu Gly Ile Ile Thr Leu Thr Asn 
                645                 650                 655     


Ser Ser Phe Leu Ala Gly Val Tyr Tyr Thr Ser Asp Ser Gly Gln Leu 
            660                 665                 670         


Leu Ala Phe Lys Asn Val Thr Ser Gly Ala Val Tyr Ser Val Thr Pro 
        675                 680                 685             


Cys Ser Phe Ser Glu Gln Ala Ala Tyr Val Asp Asp Asp Ile Val Gly 
    690                 695                 700                 


Val Ile Ser Ser Leu Ser Ser Ser Thr Phe Asn Ser Thr Arg Glu Leu 
705                 710                 715                 720 


Pro Gly Phe Phe Tyr His Ser Asn Asp Gly Ser Asn Cys Thr Glu Pro 
                725                 730                 735     


Val Leu Val Tyr Ser Asn Ile Gly Val Cys Lys Ser Gly Ser Ile Gly 
            740                 745                 750         


Tyr Val Pro Ser Gln Ser Gly Gln Val Lys Ile Ala Pro Thr Val Thr 
        755                 760                 765             


Gly Asn Ile Ser Ile Pro Thr Asn Phe Ser Met Ser Ile Arg Thr Glu 
    770                 775                 780                 


Tyr Leu Gln Leu Tyr Asn Thr Pro Val Ser Val Asp Cys Ala Thr Tyr 
785                 790                 795                 800 


Val Cys Asn Gly Asn Ser Arg Cys Lys Gln Leu Leu Thr Gln Tyr Thr 
                805                 810                 815     


Ala Ala Cys Lys Thr Ile Glu Ser Ala Leu Gln Leu Ser Ala Arg Leu 
            820                 825                 830         


Glu Ser Val Glu Val Asn Ser Met Leu Thr Ile Ser Glu Glu Ala Leu 
        835                 840                 845             


Gln Leu Ala Thr Ile Ser Ser Phe Asn Gly Asp Gly Tyr Asn Phe Thr 
    850                 855                 860                 


Asn Val Leu Gly Val Ser Val Tyr Asp Pro Ala Ser Gly Arg Val Val 
865                 870                 875                 880 


Gln Lys Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Val Thr 
                885                 890                 895     


Asn Gly Leu Gly Thr Val Asp Glu Asp Tyr Lys Arg Cys Ser Asn Gly 
            900                 905                 910         


Arg Ser Val Ala Asp Leu Val Cys Ala Gln Tyr Tyr Ser Gly Val Met 
        915                 920                 925             


Val Leu Pro Gly Val Val Asp Ala Glu Lys Leu His Met Tyr Ser Ala 
    930                 935                 940                 


Ser Leu Ile Gly Gly Met Val Leu Gly Gly Phe Thr Ala Ala Ala Leu 
945                 950                 955                 960 


Pro Phe Ser Tyr Ala Val Gln Ala Arg Leu Asn Tyr Leu Ala Leu Gln 
                965                 970                 975     


Thr Asp Val Leu Gln Arg Asn Gln Gln Leu Leu Ala Glu Ser Phe Asn 
            980                 985                 990         


Ser Ala Ile Gly Asn Ile Thr Ser  Ala Phe Glu Ser Val  Lys Glu Ala 
        995                 1000                 1005             


Ile Ser  Gln Thr Ser Lys Gly  Leu Asn Thr Val Ala  His Ala Leu 
    1010                 1015                 1020             


Thr Lys  Val Gln Glu Val Val  Asn Ser Gln Gly Ala  Ala Leu Thr 
    1025                 1030                 1035             


Gln Leu  Thr Val Gln Leu Gln  His Asn Phe Gln Ala  Ile Ser Ser 
    1040                 1045                 1050             


Ser Ile  Asp Asp Ile Tyr Ser  Arg Leu Asp Ile Leu  Ser Ala Asp 
    1055                 1060                 1065             


Val Gln  Val Asp Arg Leu Ile  Thr Gly Arg Leu Ser  Ala Leu Asn 
    1070                 1075                 1080             


Ala Phe  Val Ala Gln Thr Leu  Thr Lys Tyr Thr Glu  Val Gln Ala 
    1085                 1090                 1095             


Ser Arg  Lys Leu Ala Gln Gln  Lys Val Asn Glu Cys  Val Lys Ser 
    1100                 1105                 1110             


Gln Ser  Gln Arg Tyr Gly Phe  Cys Gly Gly Asp Gly  Glu His Ile 
    1115                 1120                 1125             


Phe Ser  Leu Val Gln Ala Ala  Pro Gln Gly Leu Leu  Phe Leu His 
    1130                 1135                 1140             


Thr Val  Leu Val Pro Gly Asp  Phe Val Asp Val Ile  Ala Ile Ala 
    1145                 1150                 1155             


Gly Leu  Cys Val Asn Asp Glu  Ile Ala Leu Thr Leu  Arg Glu Pro 
    1160                 1165                 1170             


Gly Leu  Val Leu Phe Thr His  Glu Leu Gln Asn His  Thr Ala Thr 
    1175                 1180                 1185             


Glu Tyr  Phe Val Ser Ser Arg  Arg Met Phe Glu Pro  Arg Lys Pro 
    1190                 1195                 1200             


Thr Val  Ser Asp Phe Val Gln  Ile Glu Ser Cys Val  Val Thr Tyr 
    1205                 1210                 1215             


Val Asn  Leu Thr Arg Asp Gln  Leu Pro Asp Val Ile  Pro Asp Tyr 
    1220                 1225                 1230             


Ile Asp  Val Asn Lys Thr Leu  Asp Glu Ile Leu Ala  Ser Leu Pro 
    1235                 1240                 1245             


Asn Arg  Thr Gly Pro Ser Leu  Pro Leu Asp Val Phe  Asn Ala Thr 
    1250                 1255                 1260             


Tyr Leu  Asn Leu Thr Gly Glu  Ile Ala Asp Leu Glu  Gln Arg Ser 
    1265                 1270                 1275             


Glu Ser  Leu Arg Asn Thr Thr  Glu Glu Leu Gln Ser  Leu Ile Tyr 
    1280                 1285                 1290             


Asn Ile  Asn Asn Thr Leu Val  Asp Leu Glu Trp Leu  Asn Arg Val 
    1295                 1300                 1305             


Glu Thr  Tyr Ile Lys Trp Pro  Trp Trp Val Trp Leu  Ile Ile Phe 
    1310                 1315                 1320             


Ile Val  Leu Ile Phe Val Val  Ser Leu Leu Val Phe  Cys Cys Ile 
    1325                 1330                 1335             


Ser Thr  Gly Cys Cys Gly Cys  Cys Gly Cys Cys Gly  Ala Cys Phe 
    1340                 1345                 1350             


Ser Gly  Cys Cys Arg Gly Pro  Arg Leu Gln Pro Tyr  Glu Phe Glu 
    1355                 1360                 1365             


Lys Val  His Val Gln 
    1370             


<210>  3
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Dominant T cell epitope

<400>  3

Lys Val Asp Thr Tyr Asn Ser Thr Val Tyr Lys Phe Leu Ala Val 
1               5                   10                  15  


<210>  4
<211>  4110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PEDV-S 64plus consensus

<400>  4
ctgcctcagg atgtcacaag atgctctgct aataccaact tcaggagatt ctttagcaag       60

tttaatgtgc aggcaccagc tgtggtcgtg ctcggcggat acctgcctat cggggagaat      120

cagggcgtga actccacttg gtattgcgct ggccagcacc ctaccgcatc tggagtgcat      180

gggatcttcc tgtcacacat tcggggaggg catgggttcg agatcggcat tagccaggag      240

ccatttgacc cttccgggta ccagctgtat ctccacaagg ccaccaacgg caatacaaac      300

gcaactgccc gactgaggat ctgccagttc cctagcatta agaccctggg gcccacagcc      360

aacaatgacg tgaccacagg ccgcaattgt ctgtttaaca aagctattcc cgcacacatg      420

tccgatggcg agcattctgt cgtgggaatc acttgggaca acgatagggt caccgtgttc      480

tccgacaaga tctactattt ctactttaaa aacgattgga gcagagtggc caccaagtgc      540

tataattccg gcggatgtgc tatgcagtac gtctatgagc ccacatacta tatgctgaac      600

gtgacttctg ccggcgaaga cggaatttca taccagccat gcaccgccaa ttgtatcggc      660

tatgccgcta acgtgttcgc tacagagccc aatggccaca tcccagaagg attcagcttt      720

aacaattggt ttctgctctc taacgattca accctggtgc atggcaaggt cgtgtccaat      780

cagcccctgc tcgtgaactg cctgctcgcc atcccaaaaa tctacggact ggggcagttc      840

tttagcttca accagaccat cgacggagtc tgtaatggag cagcagtgca gcgagcacca      900

gaggctctga ggttcaatat caacgataca agtgtcattc tggctgaagg aagcatcgtg      960

ctccacactg cactggggac caacctctca ttcgtgtgca gtaatagctc cgaccctcat     1020

ctggcaacat ttgccatccc actcggcgcc actcaggtcc cttactattg tttcctgaag     1080

gtggatacat acaactccac tgtctataaa tttctggccg tgctgccccc aaccgtcagg     1140

gagatcgtga ttacaaagta cggcgacgtg tacgtgaacg gcttcggata cctgcacctc     1200

ggactgctcg atgccgtgac catcaacttc accgggcatg gcactgacga tgacgtgtcc     1260

ggcttctgga ccattgcatc tacaaacttt gtcgacgccc tgatcgaggt gcagggaacc     1320

gccatccaga gaattctgta ctgcgatgac cctgtctcac agctcaaatg tagtcaggtg     1380

gctttcgacc tggatgacgg cttttatccc atttcttcac ggaacctgct cagtcacgag     1440

cagcccatca gcttcgtgac cctgccaagc ttcaatgatc actcctttgt caacatcaca     1500

gtgtcagcca gttttggggg ccattcagga gccaacctga ttgctagtga cactaccatc     1560

aatgggttca gtagcttttg cgtggatact agacagttca ccatcagtct gttttacaat     1620

gtcaccaact catacggcta tgtgagcaag tcccaggaca gcaactgtcc cttcacactg     1680

cagtccgtca acgattacct ctctttctca aagttctgcg tgagtaccag cctgctcgct     1740

tctgcatgta caatcgacct gttcgggtac ccagagtttg gctccggcgt gaagttcact     1800

tccctgtatt tccagtttac caagggagag ctgatcaccg ggacacctaa acccctcgaa     1860

ggggtcaccg acgtgagctt catgacactg gacgtgtgca ccaagtacac aatctacggc     1920

ttcaaaggag aggggatcat tactctgacc aacagtagct ttctcgccgg cgtgtactat     1980

acctccgact ctggacagct gctcgccttc aagaatgtga cttctggcgc tgtctactca     2040

gtgaccccat gtagcttcag cgagcaggct gcatatgtgg atgacgatat cgtcggagtg     2100

atttcctctc tgtcaagtag cacattcaac agcactaggg agctgccagg gttcttttac     2160

cacagcaatg acggctccaa ctgcaccgaa cctgtcctgg tgtactccaa cattggcgtc     2220

tgtaagagcg ggtccatcgg ctatgtgcct tctcagtcag gacaggtcaa aatcgcccct     2280

acagtgactg ggaatatcag cattcccaca aacttcagta tgagcattag aactgagtac     2340

ctgcagctct ataacacccc cgtctctgtg gactgcgcca catacgtgtg caatggcaac     2400

agccgatgca agcagctgct cactcagtat accgccgctt gtaaaaccat cgaaagtgct     2460

ctgcagctca gcgcaaggct ggagtccgtc gaagtgaact ctatgctgac aatttcagag     2520

gaagcactgc agctcgccac tatctcctct ttcaatggcg acggatacaa cttcaccaac     2580

gtgctgggcg tctccgtgta tgatcccgcc tctggaagag tcgtgcagaa gcggtccttc     2640

atcgaggacc tgctgttcaa caaggtcgtg accaacgggc tgggcacagt ggacgaagat     2700

tacaagaggt gctctaacgg ccgatcagtc gcagacctgg tgtgcgctca gtactatagc     2760

ggggtcatgg tgctgccagg agtcgtggat gcagagaaac tgcacatgta ctccgcctct     2820

ctgatcggag ggatggtgct cggcggattc accgcagcag ccgctctgcc tttttcctac     2880

gctgtgcagg caagactgaa ctatctggcc ctccagacag acgtgctgca gcggaatcag     2940

cagctgctcg ccgagagttt caatagcgcc atcggaaaca ttaccagtgc ttttgagagc     3000

gtgaaggaag caatctccca gacttctaaa gggctgaaca ccgtcgccca cgctctcaca     3060

aaggtgcagg aagtcgtgaa tagccaggga gcagccctga cacagctcac tgtgcagctg     3120

cagcacaact tccaggccat ctcaagtagc atcgacgata tctacagcag gctggacatt     3180

ctcagtgctg acgtgcaggt ggataggctg atcaccggca gactgtccgc cctcaacgct     3240

ttcgtcgcac agaccctgac aaagtacaca gaggtgcagg catctcgcaa gctggcccag     3300

cagaaagtca acgaatgcgt gaaatcacag agtcagcgat acggattctg tgggggcgac     3360

ggggagcaca tcttttccct ggtgcaggct gcacctcagg gactgctctt cctgcatacc     3420

gtcctcgtgc ccggcgactt tgtcgatgtg atcgcaattg ccggactgtg cgtgaacgat     3480

gagatcgccc tgaccctccg agaaccagga ctggtgctct tcacacacga gctgcagaat     3540

catactgcta ccgaatactt cgtgtcctct cggcgcatgt ttgagccccg gaagccaacc     3600

gtcagcgact tcgtgcagat cgaatcctgc gtcgtgactt acgtgaacct gacccgcgac     3660

cagctcccag atgtcatccc tgactatatt gatgtgaaca aaactctgga tgagatcctg     3720

gcctctctcc ccaatcgaac cggacctagc ctgccactcg acgtgttcaa tgcaacatac     3780

ctgaacctca ctggggaaat cgctgatctg gagcagcgga gcgaatccct ccgcaacaca     3840

actgaggaac tgcagtccct catctacaat attaacaata cactggtcga cctggagtgg     3900

ctgaaccgag tggaaactta tatcaagtgg ccatggtggg tctggctgat cattttcatc     3960

gtgctcattt ttgtcgtgtc cctgctcgtg ttctgctgta tttctaccgg atgctgtggg     4020

tgctgtggct gctgtggagc ctgcttttca ggatgttgtc gggggccaag actccagcca     4080

tacgaagcat ttgaaaaggt ccacgtccag                                      4110


<210>  5
<211>  1370
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PEDV-S 64plus consensus

<400>  5

Leu Pro Gln Asp Val Thr Arg Cys Ser Ala Asn Thr Asn Phe Arg Arg 
1               5                   10                  15      


Phe Phe Ser Lys Phe Asn Val Gln Ala Pro Ala Val Val Val Leu Gly 
            20                  25                  30          


Gly Tyr Leu Pro Ile Gly Glu Asn Gln Gly Val Asn Ser Thr Trp Tyr 
        35                  40                  45              


Cys Ala Gly Gln His Pro Thr Ala Ser Gly Val His Gly Ile Phe Leu 
    50                  55                  60                  


Ser His Ile Arg Gly Gly His Gly Phe Glu Ile Gly Ile Ser Gln Glu 
65                  70                  75                  80  


Pro Phe Asp Pro Ser Gly Tyr Gln Leu Tyr Leu His Lys Ala Thr Asn 
                85                  90                  95      


Gly Asn Thr Asn Ala Thr Ala Arg Leu Arg Ile Cys Gln Phe Pro Ser 
            100                 105                 110         


Ile Lys Thr Leu Gly Pro Thr Ala Asn Asn Asp Val Thr Thr Gly Arg 
        115                 120                 125             


Asn Cys Leu Phe Asn Lys Ala Ile Pro Ala His Met Ser Asp Gly Glu 
    130                 135                 140                 


His Ser Val Val Gly Ile Thr Trp Asp Asn Asp Arg Val Thr Val Phe 
145                 150                 155                 160 


Ser Asp Lys Ile Tyr Tyr Phe Tyr Phe Lys Asn Asp Trp Ser Arg Val 
                165                 170                 175     


Ala Thr Lys Cys Tyr Asn Ser Gly Gly Cys Ala Met Gln Tyr Val Tyr 
            180                 185                 190         


Glu Pro Thr Tyr Tyr Met Leu Asn Val Thr Ser Ala Gly Glu Asp Gly 
        195                 200                 205             


Ile Ser Tyr Gln Pro Cys Thr Ala Asn Cys Ile Gly Tyr Ala Ala Asn 
    210                 215                 220                 


Val Phe Ala Thr Glu Pro Asn Gly His Ile Pro Glu Gly Phe Ser Phe 
225                 230                 235                 240 


Asn Asn Trp Phe Leu Leu Ser Asn Asp Ser Thr Leu Val His Gly Lys 
                245                 250                 255     


Val Val Ser Asn Gln Pro Leu Leu Val Asn Cys Leu Leu Ala Ile Pro 
            260                 265                 270         


Lys Ile Tyr Gly Leu Gly Gln Phe Phe Ser Phe Asn Gln Thr Ile Asp 
        275                 280                 285             


Gly Val Cys Asn Gly Ala Ala Val Gln Arg Ala Pro Glu Ala Leu Arg 
    290                 295                 300                 


Phe Asn Ile Asn Asp Thr Ser Val Ile Leu Ala Glu Gly Ser Ile Val 
305                 310                 315                 320 


Leu His Thr Ala Leu Gly Thr Asn Leu Ser Phe Val Cys Ser Asn Ser 
                325                 330                 335     


Ser Asp Pro His Leu Ala Thr Phe Ala Ile Pro Leu Gly Ala Thr Gln 
            340                 345                 350         


Val Pro Tyr Tyr Cys Phe Leu Lys Val Asp Thr Tyr Asn Ser Thr Val 
        355                 360                 365             


Tyr Lys Phe Leu Ala Val Leu Pro Pro Thr Val Arg Glu Ile Val Ile 
    370                 375                 380                 


Thr Lys Tyr Gly Asp Val Tyr Val Asn Gly Phe Gly Tyr Leu His Leu 
385                 390                 395                 400 


Gly Leu Leu Asp Ala Val Thr Ile Asn Phe Thr Gly His Gly Thr Asp 
                405                 410                 415     


Asp Asp Val Ser Gly Phe Trp Thr Ile Ala Ser Thr Asn Phe Val Asp 
            420                 425                 430         


Ala Leu Ile Glu Val Gln Gly Thr Ala Ile Gln Arg Ile Leu Tyr Cys 
        435                 440                 445             


Asp Asp Pro Val Ser Gln Leu Lys Cys Ser Gln Val Ala Phe Asp Leu 
    450                 455                 460                 


Asp Asp Gly Phe Tyr Pro Ile Ser Ser Arg Asn Leu Leu Ser His Glu 
465                 470                 475                 480 


Gln Pro Ile Ser Phe Val Thr Leu Pro Ser Phe Asn Asp His Ser Phe 
                485                 490                 495     


Val Asn Ile Thr Val Ser Ala Ser Phe Gly Gly His Ser Gly Ala Asn 
            500                 505                 510         


Leu Ile Ala Ser Asp Thr Thr Ile Asn Gly Phe Ser Ser Phe Cys Val 
        515                 520                 525             


Asp Thr Arg Gln Phe Thr Ile Ser Leu Phe Tyr Asn Val Thr Asn Ser 
    530                 535                 540                 


Tyr Gly Tyr Val Ser Lys Ser Gln Asp Ser Asn Cys Pro Phe Thr Leu 
545                 550                 555                 560 


Gln Ser Val Asn Asp Tyr Leu Ser Phe Ser Lys Phe Cys Val Ser Thr 
                565                 570                 575     


Ser Leu Leu Ala Ser Ala Cys Thr Ile Asp Leu Phe Gly Tyr Pro Glu 
            580                 585                 590         


Phe Gly Ser Gly Val Lys Phe Thr Ser Leu Tyr Phe Gln Phe Thr Lys 
        595                 600                 605             


Gly Glu Leu Ile Thr Gly Thr Pro Lys Pro Leu Glu Gly Val Thr Asp 
    610                 615                 620                 


Val Ser Phe Met Thr Leu Asp Val Cys Thr Lys Tyr Thr Ile Tyr Gly 
625                 630                 635                 640 


Phe Lys Gly Glu Gly Ile Ile Thr Leu Thr Asn Ser Ser Phe Leu Ala 
                645                 650                 655     


Gly Val Tyr Tyr Thr Ser Asp Ser Gly Gln Leu Leu Ala Phe Lys Asn 
            660                 665                 670         


Val Thr Ser Gly Ala Val Tyr Ser Val Thr Pro Cys Ser Phe Ser Glu 
        675                 680                 685             


Gln Ala Ala Tyr Val Asp Asp Asp Ile Val Gly Val Ile Ser Ser Leu 
    690                 695                 700                 


Ser Ser Ser Thr Phe Asn Ser Thr Arg Glu Leu Pro Gly Phe Phe Tyr 
705                 710                 715                 720 


His Ser Asn Asp Gly Ser Asn Cys Thr Glu Pro Val Leu Val Tyr Ser 
                725                 730                 735     


Asn Ile Gly Val Cys Lys Ser Gly Ser Ile Gly Tyr Val Pro Ser Gln 
            740                 745                 750         


Ser Gly Gln Val Lys Ile Ala Pro Thr Val Thr Gly Asn Ile Ser Ile 
        755                 760                 765             


Pro Thr Asn Phe Ser Met Ser Ile Arg Thr Glu Tyr Leu Gln Leu Tyr 
    770                 775                 780                 


Asn Thr Pro Val Ser Val Asp Cys Ala Thr Tyr Val Cys Asn Gly Asn 
785                 790                 795                 800 


Ser Arg Cys Lys Gln Leu Leu Thr Gln Tyr Thr Ala Ala Cys Lys Thr 
                805                 810                 815     


Ile Glu Ser Ala Leu Gln Leu Ser Ala Arg Leu Glu Ser Val Glu Val 
            820                 825                 830         


Asn Ser Met Leu Thr Ile Ser Glu Glu Ala Leu Gln Leu Ala Thr Ile 
        835                 840                 845             


Ser Ser Phe Asn Gly Asp Gly Tyr Asn Phe Thr Asn Val Leu Gly Val 
    850                 855                 860                 


Ser Val Tyr Asp Pro Ala Ser Gly Arg Val Val Gln Lys Arg Ser Phe 
865                 870                 875                 880 


Ile Glu Asp Leu Leu Phe Asn Lys Val Val Thr Asn Gly Leu Gly Thr 
                885                 890                 895     


Val Asp Glu Asp Tyr Lys Arg Cys Ser Asn Gly Arg Ser Val Ala Asp 
            900                 905                 910         


Leu Val Cys Ala Gln Tyr Tyr Ser Gly Val Met Val Leu Pro Gly Val 
        915                 920                 925             


Val Asp Ala Glu Lys Leu His Met Tyr Ser Ala Ser Leu Ile Gly Gly 
    930                 935                 940                 


Met Val Leu Gly Gly Phe Thr Ala Ala Ala Ala Leu Pro Phe Ser Tyr 
945                 950                 955                 960 


Ala Val Gln Ala Arg Leu Asn Tyr Leu Ala Leu Gln Thr Asp Val Leu 
                965                 970                 975     


Gln Arg Asn Gln Gln Leu Leu Ala Glu Ser Phe Asn Ser Ala Ile Gly 
            980                 985                 990         


Asn Ile Thr Ser Ala Phe Glu Ser  Val Lys Glu Ala Ile  Ser Gln Thr 
        995                 1000                 1005             


Ser Lys  Gly Leu Asn Thr Val  Ala His Ala Leu Thr  Lys Val Gln 
    1010                 1015                 1020             


Glu Val  Val Asn Ser Gln Gly  Ala Ala Leu Thr Gln  Leu Thr Val 
    1025                 1030                 1035             


Gln Leu  Gln His Asn Phe Gln  Ala Ile Ser Ser Ser  Ile Asp Asp 
    1040                 1045                 1050             


Ile Tyr  Ser Arg Leu Asp Ile  Leu Ser Ala Asp Val  Gln Val Asp 
    1055                 1060                 1065             


Arg Leu  Ile Thr Gly Arg Leu  Ser Ala Leu Asn Ala  Phe Val Ala 
    1070                 1075                 1080             


Gln Thr  Leu Thr Lys Tyr Thr  Glu Val Gln Ala Ser  Arg Lys Leu 
    1085                 1090                 1095             


Ala Gln  Gln Lys Val Asn Glu  Cys Val Lys Ser Gln  Ser Gln Arg 
    1100                 1105                 1110             


Tyr Gly  Phe Cys Gly Gly Asp  Gly Glu His Ile Phe  Ser Leu Val 
    1115                 1120                 1125             


Gln Ala  Ala Pro Gln Gly Leu  Leu Phe Leu His Thr  Val Leu Val 
    1130                 1135                 1140             


Pro Gly  Asp Phe Val Asp Val  Ile Ala Ile Ala Gly  Leu Cys Val 
    1145                 1150                 1155             


Asn Asp  Glu Ile Ala Leu Thr  Leu Arg Glu Pro Gly  Leu Val Leu 
    1160                 1165                 1170             


Phe Thr  His Glu Leu Gln Asn  His Thr Ala Thr Glu  Tyr Phe Val 
    1175                 1180                 1185             


Ser Ser  Arg Arg Met Phe Glu  Pro Arg Lys Pro Thr  Val Ser Asp 
    1190                 1195                 1200             


Phe Val  Gln Ile Glu Ser Cys  Val Val Thr Tyr Val  Asn Leu Thr 
    1205                 1210                 1215             


Arg Asp  Gln Leu Pro Asp Val  Ile Pro Asp Tyr Ile  Asp Val Asn 
    1220                 1225                 1230             


Lys Thr  Leu Asp Glu Ile Leu  Ala Ser Leu Pro Asn  Arg Thr Gly 
    1235                 1240                 1245             


Pro Ser  Leu Pro Leu Asp Val  Phe Asn Ala Thr Tyr  Leu Asn Leu 
    1250                 1255                 1260             


Thr Gly  Glu Ile Ala Asp Leu  Glu Gln Arg Ser Glu  Ser Leu Arg 
    1265                 1270                 1275             


Asn Thr  Thr Glu Glu Leu Gln  Ser Leu Ile Tyr Asn  Ile Asn Asn 
    1280                 1285                 1290             


Thr Leu  Val Asp Leu Glu Trp  Leu Asn Arg Val Glu  Thr Tyr Ile 
    1295                 1300                 1305             


Lys Trp  Pro Trp Trp Val Trp  Leu Ile Ile Phe Ile  Val Leu Ile 
    1310                 1315                 1320             


Phe Val  Val Ser Leu Leu Val  Phe Cys Cys Ile Ser  Thr Gly Cys 
    1325                 1330                 1335             


Cys Gly  Cys Cys Gly Cys Cys  Gly Ala Cys Phe Ser  Gly Cys Cys 
    1340                 1345                 1350             


Arg Gly  Pro Arg Leu Gln Pro  Tyr Glu Ala Phe Glu  Lys Val His 
    1355                 1360                 1365             


Val Gln  
    1370 


<210>  6
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Native leader

<400>  6
atgaaatcac tcacttactt ctggctgttt ctccccgtgc tctcaaccct ctct             54


<210>  7
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Native leader

<400>  7

Met Lys Ser Leu Thr Tyr Phe Trp Leu Phe Leu Pro Val Leu Ser Thr 
1               5                   10                  15      


Leu Ser 
        


<210>  8
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IgE leader

<400>  8
atggactgga cttggattct gtttctggtg gccgctgcta cccgagtgca ttct             54


<210>  9
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgE leader

<400>  9

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser 
        


