                         SEQUENCE LISTING

<110>  FRED HUTCHINSON CANCER RESEARCH CENTER
 
<120>  COCAL ENVELOPE PSEUDOTYPED RETROVIRAL VECTOR PRODUCER CELLS

<130>  F053-0016PCT

<150>  US 62/106184
<151>  2015-01-21

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  1652
<212>  DNA
<213>  Cocal virus

<400>  1
aacagtgatc aactgttcca gattcaacat gaatttccta ctcttgacat ttattgtgtt       60

gccgttgtgc agccacgcca agttctccat tgtattccct caaagccaaa aaggcaattg      120

gaagaatgta ccatcatctt accattactg cccttcaagt tcggatcaaa actggcacaa      180

tgatttgctt ggaatcacaa tgaaagtcaa aatgcccaaa acacacaaag ctattcaagc      240

agacgggtgg atgtgtcatg ctgccaaatg gatcactacc tgtgactttc gctggtacgg      300

acccaaatac atcactcact ccattcattc catccagcct acttcagagc agtgtaaaga      360

aagcatcaag caaacaaaac aaggtacttg gatgagtcct ggcttccctc cacagaactg      420

cgggtatgca acagtaacag actctgtcgc tgttgtcgtc caagccactc ctcatcatgt      480

cttggttgat gaatatactg gagaatggat cgactctcaa ttccccaacg ggaaatgtga      540

aaccgaagag tgcgagaccg tccacaactc taccgtatgg tactctgact acaaagtaac      600

tggattatgt gacgcaactc tggtagacac agagatcacc ttcttctctg aagatggcaa      660

aaaagaatct atcgggaagc ccaacacagg ctataggagc aactacttcg cttatgagaa      720

aggggacaaa gtatgtaaaa tgaactactg caagcatgcg ggtgtgaggt tgccttccgg      780

ggtttggttt gagtttgtgg atcaggatgt ctacgccgcc gccaaacttc cagaatgccc      840

cgttggtgcc actatctccg ctccgacaca gacctctgtt gacgtaagtc tcattctaga      900

tgtagagaga attttagatt actctctgtg tcaagagaca tggagcaaga tccggtccaa      960

acagccagta tcccctgttg accttagtta cttggccccc aagaatcctg ggaccggacc     1020

ggcattcaca atcatcaatg gcactctgaa gtactttgag accagataca ttcggattga     1080

tatagacaat ccaatcatct ccaagatggt ggggaaaata agtggcagtc aaacagaacg     1140

agaattgtgg acagagtggt tcccctacga gggtgtcgag atagggccaa atgggattct     1200

caaaacccct acaggataca aattcccact cttcatgata ggacacggga tgctagattc     1260

cgacttgcac aagacgtccc aagcagaggt ctttgaacat cctcaccttg cagaagcacc     1320

aaagcagttg ccggaggagg agactttatt ttttggtgac acaggaatct ccaaaaatcc     1380

ggtcgaactg attgaagggt ggtttagtag ttggaagagc actgtagtca cctttttctt     1440

tgccatagga gtatttatac tactgtatgt agtggccaga attgtgatcg cagtgagata     1500

cagatatcaa ggctcaaata acaaaagaat ttacaatgat attgagatga gcagatttag     1560

aaaatgatga agaccctcag atgattatac atatgtgata tgaaaaaaac taacagtcat     1620

catggacttg aatgacttcg agttgagaca gt                                   1652


<210>  2
<211>  512
<212>  PRT
<213>  Cocal Virus

<400>  2

Met Asn Phe Leu Leu Leu Thr Phe Ile Val Leu Pro Leu Cys Ser His 
1               5                   10                  15      


Ala Lys Phe Ser Ile Val Phe Pro Gln Ser Gln Lys Gly Asn Trp Lys 
            20                  25                  30          


Asn Val Pro Ser Ser Tyr His Tyr Cys Pro Ser Ser Ser Asp Gln Asn 
        35                  40                  45              


Trp His Asn Asp Leu Leu Gly Ile Thr Met Lys Val Lys Met Pro Lys 
    50                  55                  60                  


Thr His Lys Ala Ile Gln Ala Asp Gly Trp Met Cys His Ala Ala Lys 
65                  70                  75                  80  


Trp Ile Thr Thr Cys Asp Phe Arg Trp Tyr Gly Pro Lys Tyr Ile Thr 
                85                  90                  95      


His Ser Ile His Ser Ile Gln Pro Thr Ser Glu Gln Cys Lys Glu Ser 
            100                 105                 110         


Ile Lys Gln Thr Lys Gln Gly Thr Trp Met Ser Pro Gly Phe Pro Pro 
        115                 120                 125             


Gln Asn Cys Gly Tyr Ala Thr Val Thr Asp Ser Val Ala Val Val Val 
    130                 135                 140                 


Gln Ala Thr Pro His His Val Leu Val Asp Glu Tyr Thr Gly Glu Trp 
145                 150                 155                 160 


Ile Asp Ser Gln Phe Pro Asn Gly Lys Cys Glu Thr Glu Glu Cys Glu 
                165                 170                 175     


Thr Val His Asn Ser Thr Val Trp Tyr Ser Asp Tyr Lys Val Thr Gly 
            180                 185                 190         


Leu Cys Asp Ala Thr Leu Val Asp Thr Glu Ile Thr Phe Phe Ser Glu 
        195                 200                 205             


Asp Gly Lys Lys Glu Ser Ile Gly Lys Pro Asn Thr Gly Tyr Arg Ser 
    210                 215                 220                 


Asn Tyr Phe Ala Tyr Glu Lys Gly Asp Lys Val Cys Lys Met Asn Tyr 
225                 230                 235                 240 


Cys Lys His Ala Gly Val Arg Leu Pro Ser Gly Val Trp Phe Glu Phe 
                245                 250                 255     


Val Asp Gln Asp Val Tyr Ala Ala Ala Lys Leu Pro Glu Cys Pro Val 
            260                 265                 270         


Gly Ala Thr Ile Ser Ala Pro Thr Gln Thr Ser Val Asp Val Ser Leu 
        275                 280                 285             


Ile Leu Asp Val Glu Arg Ile Leu Asp Tyr Ser Leu Cys Gln Glu Thr 
    290                 295                 300                 


Trp Ser Lys Ile Arg Ser Lys Gln Pro Val Ser Pro Val Asp Leu Ser 
305                 310                 315                 320 


Tyr Leu Ala Pro Lys Asn Pro Gly Thr Gly Pro Ala Phe Thr Ile Ile 
                325                 330                 335     


Asn Gly Thr Leu Lys Tyr Phe Glu Thr Arg Tyr Ile Arg Ile Asp Ile 
            340                 345                 350         


Asp Asn Pro Ile Ile Ser Lys Met Val Gly Lys Ile Ser Gly Ser Gln 
        355                 360                 365             


Thr Glu Arg Glu Leu Trp Thr Glu Trp Phe Pro Tyr Glu Gly Val Glu 
    370                 375                 380                 


Ile Gly Pro Asn Gly Ile Leu Lys Thr Pro Thr Gly Tyr Lys Phe Pro 
385                 390                 395                 400 


Leu Phe Met Ile Gly His Gly Met Leu Asp Ser Asp Leu His Lys Thr 
                405                 410                 415     


Ser Gln Ala Glu Val Phe Glu His Pro His Leu Ala Glu Ala Pro Lys 
            420                 425                 430         


Gln Leu Pro Glu Glu Glu Thr Leu Phe Phe Gly Asp Thr Gly Ile Ser 
        435                 440                 445             


Lys Asn Pro Val Glu Leu Ile Glu Gly Trp Phe Ser Ser Trp Lys Ser 
    450                 455                 460                 


Thr Val Val Thr Phe Phe Phe Ala Ile Gly Val Phe Ile Leu Leu Tyr 
465                 470                 475                 480 


Val Val Ala Arg Ile Val Ile Ala Val Arg Tyr Arg Tyr Gln Gly Ser 
                485                 490                 495     


Asn Asn Lys Arg Ile Tyr Asn Asp Ile Glu Met Ser Arg Phe Arg Lys 
            500                 505                 510         


<210>  3
<211>  1539
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide Sequence of the human codon enriched Cocal 
       vesiculovirus envelope glycoprotein

<400>  3
atgaattttc ttctcttgac ctttatcgtc cttccgctct gcagtcacgc taaattttcg       60

atcgtcttcc cacagagtca gaagggcaat tggaagaatg taccgagttc atatcattat      120

tgtccaagca gctctgatca aaactggcat aacgacctgc tgggcattac catgaaggtg      180

aaaatgccta agacacataa ggcgattcag gcagacgggt ggatgtgcca cgcagccaag      240

tggattacaa cttgtgactt ccgatggtac ggtcctaagt atattactca ctccatacac      300

agcatccagc ccaccagtga gcagtgcaaa gagagtatca agcagaccaa gcagggaacc      360

tggatgtcac ctggctttcc acctcagaat tgtggctatg caacagtgac agactcagtg      420

gctgttgtgg tgcaggcaac cccccaccac gtactcgttg acgaatatac aggcgaatgg      480

attgactccc agtttcccaa cggtaaatgc gagacagaag agtgcgagac tgtgcacaat      540

tcaacagtgt ggtactccga ttataaggtt accgggcttt gcgacgccac actggtggac      600

acagagataa cgtttttttc cgaagacgga aaaaaggaaa gtatcgggaa acccaacact      660

ggataccgga gcaattactt cgcgtatgag aaaggagata aggtctgcaa aatgaattat      720

tgcaaacacg ccggggtaag gctgccctcc ggcgtgtggt ttgagttcgt ggaccaggac      780

gtctacgcag ccgccaaatt gcccgagtgt ccagtgggag ctacaatttc cgcaccgaca      840

caaacctcag tggatgtgtc tctgattctg gacgtagaga ggatcctcga ctactctttg      900

tgtcaggaga cgtggagcaa gatacggtct aagcaaccag tctcacccgt agatttgagc      960

tacctcgccc cgaaaaaccc aggcacgggc ccagcgttca cgatcatcaa cggcacgctt     1020

aaatatttcg agactcgcta tatccgcatc gacatcgaca atcctatcat ctctaagatg     1080

gtgggtaaga tctctggatc ccagactgaa cgagaactgt ggacagaatg gttcccctac     1140

gagggcgtcg agattggccc taacggaata ctgaagaccc ctaccggcta taagttccct     1200

ctgtttatga tcggccacgg aatgctggat tctgatttgc ataagacttc acaagcagaa     1260

gtctttgaac atcctcacct cgccgaagca cctaaacagt tgcctgagga agagaccctg     1320

ttcttcggcg atacaggtat atccaaaaac ccggtggagc ttatcgaagg ttggtttagc     1380

agctggaagt caacagtggt aactttcttc ttcgccatcg gcgtgtttat acttctgtac     1440

gtagtggccc gcatcgtgat cgcagtgcgc tacagatacc aaggaagcaa caacaaaaga     1500

atctacaacg acatagagat gagccgcttc aggaagtga                            1539


<210>  4
<211>  512
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of human codon enriched Cocal vesiculovirus 
       envelope glycoprotein

<400>  4

Met Asn Phe Leu Leu Leu Thr Phe Ile Val Leu Pro Leu Cys Ser His 
1               5                   10                  15      


Ala Lys Phe Ser Ile Val Phe Pro Gln Ser Gln Lys Gly Asn Trp Lys 
            20                  25                  30          


Asn Val Pro Ser Ser Tyr His Tyr Cys Pro Ser Ser Ser Asp Gln Asn 
        35                  40                  45              


Trp His Asn Asp Leu Leu Gly Ile Thr Met Lys Val Lys Met Pro Lys 
    50                  55                  60                  


Thr His Lys Ala Ile Gln Ala Asp Gly Trp Met Cys His Ala Ala Lys 
65                  70                  75                  80  


Trp Ile Thr Thr Cys Asp Phe Arg Trp Tyr Gly Pro Lys Tyr Ile Thr 
                85                  90                  95      


His Ser Ile His Ser Ile Gln Pro Thr Ser Glu Gln Cys Lys Glu Ser 
            100                 105                 110         


Ile Lys Gln Thr Lys Gln Gly Thr Trp Met Ser Pro Gly Phe Pro Pro 
        115                 120                 125             


Gln Asn Cys Gly Tyr Ala Thr Val Thr Asp Ser Val Ala Val Val Val 
    130                 135                 140                 


Gln Ala Thr Pro His His Val Leu Val Asp Glu Tyr Thr Gly Glu Trp 
145                 150                 155                 160 


Ile Asp Ser Gln Phe Pro Asn Gly Lys Cys Glu Thr Glu Glu Cys Glu 
                165                 170                 175     


Thr Val His Asn Ser Thr Val Trp Tyr Ser Asp Tyr Lys Val Thr Gly 
            180                 185                 190         


Leu Cys Asp Ala Thr Leu Val Asp Thr Glu Ile Thr Phe Phe Ser Glu 
        195                 200                 205             


Asp Gly Lys Lys Glu Ser Ile Gly Lys Pro Asn Thr Gly Tyr Arg Ser 
    210                 215                 220                 


Asn Tyr Phe Ala Tyr Glu Lys Gly Asp Lys Val Cys Lys Met Asn Tyr 
225                 230                 235                 240 


Cys Lys His Ala Gly Val Arg Leu Pro Ser Gly Val Trp Phe Glu Phe 
                245                 250                 255     


Val Asp Gln Asp Val Tyr Ala Ala Ala Lys Leu Pro Glu Cys Pro Val 
            260                 265                 270         


Gly Ala Thr Ile Ser Ala Pro Thr Gln Thr Ser Val Asp Val Ser Leu 
        275                 280                 285             


Ile Leu Asp Val Glu Arg Ile Leu Asp Tyr Ser Leu Cys Gln Glu Thr 
    290                 295                 300                 


Trp Ser Lys Ile Arg Ser Lys Gln Pro Val Ser Pro Val Asp Leu Ser 
305                 310                 315                 320 


Tyr Leu Ala Pro Lys Asn Pro Gly Thr Gly Pro Ala Phe Thr Ile Ile 
                325                 330                 335     


Asn Gly Thr Leu Lys Tyr Phe Glu Thr Arg Tyr Ile Arg Ile Asp Ile 
            340                 345                 350         


Asp Asn Pro Ile Ile Ser Lys Met Val Gly Lys Ile Ser Gly Ser Gln 
        355                 360                 365             


Thr Glu Arg Glu Leu Trp Thr Glu Trp Phe Pro Tyr Glu Gly Val Glu 
    370                 375                 380                 


Ile Gly Pro Asn Gly Ile Leu Lys Thr Pro Thr Gly Tyr Lys Phe Pro 
385                 390                 395                 400 


Leu Phe Met Ile Gly His Gly Met Leu Asp Ser Asp Leu His Lys Thr 
                405                 410                 415     


Ser Gln Ala Glu Val Phe Glu His Pro His Leu Ala Glu Ala Pro Lys 
            420                 425                 430         


Gln Leu Pro Glu Glu Glu Thr Leu Phe Phe Gly Asp Thr Gly Ile Ser 
        435                 440                 445             


Lys Asn Pro Val Glu Leu Ile Glu Gly Trp Phe Ser Ser Trp Lys Ser 
    450                 455                 460                 


Thr Val Val Thr Phe Phe Phe Ala Ile Gly Val Phe Ile Leu Leu Tyr 
465                 470                 475                 480 


Val Val Ala Arg Ile Val Ile Ala Val Arg Tyr Arg Tyr Gln Gly Ser 
                485                 490                 495     


Asn Asn Lys Arg Ile Tyr Asn Asp Ile Glu Met Ser Arg Phe Arg Lys 
            500                 505                 510         


<210>  5
<211>  5722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of Cocal vector plasmid pMD2.CocalG

<400>  5
ggatcccctg agggggcccc catgggctag aggatccggc ctcggcctct gcataaataa       60

aaaaaattag tcagccatga gcttggccca ttgcatacgt tgtatccata tcataatatg      120

tacatttata ttggctcatg tccaacatta ccgccatgtt gacattgatt attgactagt      180

tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt      240

acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg      300

tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg      360

gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt      420

acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg      480

accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg      540

gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt      600

ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac      660

tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg      720

tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat      780

ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccc ctcgaagctt      840

acatgtggta ccgagctcgg atcctgagaa cttcagggtg agtctatggg acccttgatg      900

ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga gaagtaacag      960

ggtacacata ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa aatgctttct     1020

tcttttaata tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc     1080

agggcaataa tgatacaatg tatcatgcct ctttgcacca ttctaaagaa taacagtgat     1140

aatttctggg ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg     1200

taactgatgt aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct     1260

tttattttat ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta     1320

atcatgttca tacctcttat cttcctccca cagctcctgg gcaacgtgct ggtctgtgtg     1380

ctggcccatc actttggcaa agcacgtgag atctgaattc atcgatgcgg ccgccaccat     1440

gaattttctt ctcttgacct ttatcgtcct tccgctctgc agtcacgcta aattttcgat     1500

cgtcttccca cagagtcaga agggcaattg gaagaatgta ccgagttcat atcattattg     1560

tccaagcagc tctgatcaaa actggcataa cgacctgctg ggcattacca tgaaggtgaa     1620

aatgcctaag acacataagg cgattcaggc agacgggtgg atgtgccacg cagccaagtg     1680

gattacaact tgtgacttcc gatggtacgg tcctaagtat attactcact ccatacacag     1740

catccagccc accagtgagc agtgcaaaga gagtatcaag cagaccaagc agggaacctg     1800

gatgtcacct ggctttccac ctcagaattg tggctatgca acagtgacag actcagtggc     1860

tgttgtggtg caggcaaccc cccaccacgt actcgttgac gaatatacag gcgaatggat     1920

tgactcccag tttcccaacg gtaaatgcga gacagaagag tgcgagactg tgcacaattc     1980

aacagtgtgg tactccgatt ataaggttac cgggctttgc gacgccacac tggtggacac     2040

agagataacg tttttttccg aagacggaaa aaaggaaagt atcgggaaac ccaacactgg     2100

ataccggagc aattacttcg cgtatgagaa aggagataag gtctgcaaaa tgaattattg     2160

caaacacgcc ggggtaaggc tgccctccgg cgtgtggttt gagttcgtgg accaggacgt     2220

ctacgcagcc gccaaattgc ccgagtgtcc agtgggagct acaatttccg caccgacaca     2280

aacctcagtg gatgtgtctc tgattctgga cgtagagagg atcctcgact actctttgtg     2340

tcaggagacg tggagcaaga tacggtctaa gcaaccagtc tcacccgtag atttgagcta     2400

cctcgccccg aaaaacccag gcacgggccc agcgttcacg atcatcaacg gcacgcttaa     2460

atatttcgag actcgctata tccgcatcga catcgacaat cctatcatct ctaagatggt     2520

gggtaagatc tctggatccc agactgaacg agaactgtgg acagaatggt tcccctacga     2580

gggcgtcgag attggcccta acggaatact gaagacccct accggctata agttccctct     2640

gtttatgatc ggccacggaa tgctggattc tgatttgcat aagacttcac aagcagaagt     2700

ctttgaacat cctcacctcg ccgaagcacc taaacagttg cctgaggaag agaccctgtt     2760

cttcggcgat acaggtatat ccaaaaaccc ggtggagctt atcgaaggtt ggtttagcag     2820

ctggaagtca acagtggtaa ctttcttctt cgccatcggc gtgtttatac ttctgtacgt     2880

agtggcccgc atcgtgatcg cagtgcgcta cagataccaa ggaagcaaca acaaaagaat     2940

ctacaacgac atagagatga gccgcttcag gaagtgaagg cctgaattca ccccaccagt     3000

gcaggctgcc tatcagaaag tggtggctgg tgtggctaat gccctggccc acaagtttca     3060

ctaagctcgc ttccttgctg tccaatttct attaaaggtt ccttggttcc ctaagtccaa     3120

ctactaaact gggggatatt atgaagggcc ttgagcatct ggattctgcc taataaaaaa     3180

catttatttt cattgcaatg atgtatttaa attatttctg aatattttac taaaaaggga     3240

atgtgggagg tcagtgcatt taaaacataa agaaatgaag agctagttca aaccttggga     3300

aaatacacta tatcttaaac tccatgaaag aaggtgaggc tgcaaacagc taatgcacat     3360

tggcaacagc cctgatgcct atgccttatt catccctcag aaaaggattc aagtagaggc     3420

ttgatttgga ggttaaagtt tggctatgct gtattttaca ttacttattg ttttagctgt     3480

cctcatgaat gtcttttcac tacccatttg cttatcctgc atctctcagc cttgactcca     3540

ctcagttctc ttgcttagag ataccacctt tcccctgaag tgttccttcc atgttttacg     3600

gcgagatggt ttctcctcgc ctggccactc agccttagtt gtctctgttg tcttatagag     3660

gtctacttga agaaggaaaa acagggggca tggtttgact gtcctgtgag cccttcttcc     3720

ctgcctcccc cactcacagt gacccggaat ccctcgacat ggcagtctag cactagtgcg     3780

gccgcagatc tgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag     3840

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag     3900

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc     3960

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc     4020

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc     4080

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt     4140

cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg     4200

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat     4260

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     4320

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     4380

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc     4440

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta     4500

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag     4560

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga     4620

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa     4680

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa     4740

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc     4800

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga     4860

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa     4920

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt     4980

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg     5040

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc     5100

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg     5160

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag     5220

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt     5280

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt     5340

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac     5400

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac     5460

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag     5520

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa     5580

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga     5640

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc     5700

cccgaaaagt gccacctgac gt                                              5722


<210>  6
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer: Hygromycin-F-XhoI

<400>  6
gagccctcga gatgaaaaag cctgaactca cc                                     32


<210>  7
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer: Hygromycin-R-2A

<400>  7
agacttcctc tgccctctga gttagcctcc cccatctccc                             40


<210>  8
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Prime: T2A-F-Hyg

<400>  8
gggagatggg ggaggctaac tcagagggca gaggaagtc                              39


<210>  9
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer: T2A-R-VSVG

<400>  9
ctaagtacaa aaggcacttc atggggcccg gattctcctc cac                         43


<210>  10
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer: T2A-R-cocal

<400>  10
ggtcaagaga agaaaattca tggggcccgg attctcctcc ac                          42


<210>  11
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer: Cocal-F-2A

<400>  11
tggaggagaa tccgggcccc atgaattttc ttctcttgac c                           41


<210>  12
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer: Cocal-R-HindIII

<400>  12
gagccaagct ttcacttcct gaagcggctc atc                                    33


<210>  13
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer: VSVG-F-2A

<400>  13
tggaggagaa tccgggcccc atgaagtgcc ttttgtactt ag                          42


<210>  14
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer: VSVG-R-HindIII

<400>  14
gagccaagct tttactttcc aagtcggttc atc                                    33


