﻿               SEQUENCE LISTING

<110> Ludwig-Maximilians-Universität München    
      Philipps-Universität Marburg

<120> A Novel Vaccine against the Middle East Respiratory Syndrome Coronavirus (MERS-CoV)

<130> X2872 PCT

<150> EP 15 15 1594.7
<151> 2015-01-19

<160> 14

<170> BiSSAP 1.2

<210> 1
<211> 413
<212> PRT
<213> Human betacoronavirus 2c EMC/2012

<220> 
<223> N protein

<400> 1
Met Ala Ser Pro Ala Ala Pro Arg Ala Val Ser Phe Ala Asp Asn Asn 
1               5                   10                  15      
Asp Ile Thr Asn Thr Asn Leu Ser Arg Gly Arg Gly Arg Asn Pro Lys 
            20                  25                  30          
Pro Arg Ala Ala Pro Asn Asn Thr Val Ser Trp Tyr Thr Gly Leu Thr 
        35                  40                  45              
Gln His Gly Lys Val Pro Leu Thr Phe Pro Pro Gly Gln Gly Val Pro 
    50                  55                  60                  
Leu Asn Ala Asn Ser Thr Pro Ala Gln Asn Ala Gly Tyr Trp Arg Arg 
65                  70                  75                  80  
Gln Asp Arg Lys Ile Asn Thr Gly Asn Gly Ile Lys Gln Leu Ala Pro 
                85                  90                  95      
Arg Trp Tyr Phe Tyr Tyr Thr Gly Thr Gly Pro Glu Ala Ala Leu Pro 
            100                 105                 110         
Phe Arg Ala Val Lys Asp Gly Ile Val Trp Val His Glu Asp Gly Ala 
        115                 120                 125             
Thr Asp Ala Pro Ser Thr Phe Gly Thr Arg Asn Pro Asn Asn Asp Ser 
    130                 135                 140                 
Ala Ile Val Thr Gln Phe Ala Pro Gly Thr Lys Leu Pro Lys Asn Phe 
145                 150                 155                 160 
His Ile Glu Gly Thr Gly Gly Asn Ser Gln Ser Ser Ser Arg Ala Ser 
                165                 170                 175     
Ser Leu Ser Arg Asn Ser Ser Arg Ser Ser Ser Gln Gly Ser Arg Ser 
            180                 185                 190         
Gly Asn Ser Thr Arg Gly Thr Ser Pro Gly Pro Ser Gly Ile Gly Ala 
        195                 200                 205             
Val Gly Gly Asp Leu Leu Tyr Leu Asp Leu Leu Asn Arg Leu Gln Ala 
    210                 215                 220                 
Leu Glu Ser Gly Lys Val Lys Gln Ser Gln Pro Lys Val Ile Thr Lys 
225                 230                 235                 240 
Lys Asp Ala Ala Ala Ala Lys Asn Lys Met Arg His Lys Arg Thr Ser 
                245                 250                 255     
Thr Lys Ser Phe Asn Met Val Gln Ala Phe Gly Leu Arg Gly Pro Gly 
            260                 265                 270         
Asp Leu Gln Gly Asn Phe Gly Asp Leu Gln Leu Asn Lys Leu Gly Thr 
        275                 280                 285             
Glu Asp Pro Arg Trp Pro Gln Ile Ala Glu Leu Ala Pro Thr Ala Ser 
    290                 295                 300                 
Ala Phe Met Gly Met Ser Gln Phe Lys Leu Thr His Gln Asn Asn Asp 
305                 310                 315                 320 
Asp His Gly Asn Pro Val Tyr Phe Leu Arg Tyr Ser Gly Ala Ile Lys 
                325                 330                 335     
Leu Asp Pro Lys Asn Pro Asn Tyr Asn Lys Trp Leu Glu Leu Leu Glu 
            340                 345                 350         
Gln Asn Ile Asp Ala Tyr Lys Thr Phe Pro Lys Lys Glu Lys Lys Gln 
        355                 360                 365             
Lys Ala Pro Lys Glu Glu Ser Thr Asp Gln Met Ser Glu Pro Pro Lys 
    370                 375                 380                 
Glu Gln Arg Val Gln Gly Ser Ile Thr Gln Arg Thr Arg Thr Arg Pro 
385                 390                 395                 400 
Ser Val Gln Pro Gly Pro Met Ile Asp Val Asn Thr Asp 
                405                 410             

<210> 2
<211> 1242
<212> DNA
<213> Human betacoronavirus 2c EMC/2012

<220> 
<221> source
<222> 1..1242
<223> /mol_type="unassigned DNA"
      /note="N protein"
      /organism="Human betacoronavirus 2c EMC/2012"

<400> 2
atggcatccc ctgctgcacc tcgtgctgtt tcctttgccg ataacaatga tataacaaat      60

acaaacctat ctcgaggtag aggacgtaat ccaaaaccac gagctgcacc aaataacact     120

gtctcttggt acactgggct tacccaacac gggaaagtcc ctcttacctt tccacctggg     180

cagggtgtac ctcttaatgc caattctacc cctgcgcaaa atgctgggta ttggcggaga     240

caggacagaa aaattaatac cgggaatgga attaagcaac tggctcccag gtggtacttc     300

tactacactg gaactggacc cgaagcagca ctcccattcc gggctgttaa ggatggcatc     360

gtttgggtcc atgaagatgg cgccactgat gctccttcaa cttttgggac gcggaaccct     420

aacaatgatt cagctattgt tacacaattc gcgcccggta ctaagcttcc taaaaacttc     480

cacattgagg ggactggagg caatagtcaa tcatcttcaa gagcctctag cttaagcaga     540

aactcttcca gatctagttc acaaggttca agatcaggaa actctacccg cggcacttct     600

ccaggtccat ctggaatcgg agcagtagga ggtgatctac tttaccttga tcttctgaac     660

agactacaag cccttgagtc tggcaaagta aagcaatcgc agccaaaagt aatcactaag     720

aaagatgctg ctgctgctaa aaataagatg cgccacaagc gcacttccac caaaagtttc     780

aacatggtgc aagcttttgg tcttcgcgga ccaggagacc tccagggaaa ctttggtgat     840

cttcaattga ataaactcgg cactgaggac ccacgttggc cccaaattgc tgagcttgct     900

cctacagcca gtgcttttat gggtatgtcg caatttaaac ttacccatca gaacaatgat     960

gatcatggca accctgtgta cttccttcgg tacagtggag ccattaaact tgacccaaag    1020

aatcccaact acaataagtg gttggagctt cttgagcaaa atattgatgc ctacaaaacc    1080

ttccctaaga aggaaaagaa acaaaaggca ccaaaagaag aatcaacaga ccaaatgtct    1140

gaacctccaa aggagcagcg tgtgcaaggt agcatcactc agcgcactcg cacccgtcca    1200

agtgttcagc ctggtccaat gattgatgtt aacactgatt ag                       1242


<210> 3
<211> 1353
<212> PRT
<213> Human betacoronavirus 2c EMC/2012

<220> 
<223> S protein

<400> 3
Met Ile His Ser Val Phe Leu Leu Met Phe Leu Leu Thr Pro Thr Glu 
1               5                   10                  15      
Ser Tyr Val Asp Val Gly Pro Asp Ser Val Lys Ser Ala Cys Ile Glu 
            20                  25                  30          
Val Asp Ile Gln Gln Thr Phe Phe Asp Lys Thr Trp Pro Arg Pro Ile 
        35                  40                  45              
Asp Val Ser Lys Ala Asp Gly Ile Ile Tyr Pro Gln Gly Arg Thr Tyr 
    50                  55                  60                  
Ser Asn Ile Thr Ile Thr Tyr Gln Gly Leu Phe Pro Tyr Gln Gly Asp 
65                  70                  75                  80  
His Gly Asp Met Tyr Val Tyr Ser Ala Gly His Ala Thr Gly Thr Thr 
                85                  90                  95      
Pro Gln Lys Leu Phe Val Ala Asn Tyr Ser Gln Asp Val Lys Gln Phe 
            100                 105                 110         
Ala Asn Gly Phe Val Val Arg Ile Gly Ala Ala Ala Asn Ser Thr Gly 
        115                 120                 125             
Thr Val Ile Ile Ser Pro Ser Thr Ser Ala Thr Ile Arg Lys Ile Tyr 
    130                 135                 140                 
Pro Ala Phe Met Leu Gly Ser Ser Val Gly Asn Phe Ser Asp Gly Lys 
145                 150                 155                 160 
Met Gly Arg Phe Phe Asn His Thr Leu Val Leu Leu Pro Asp Gly Cys 
                165                 170                 175     
Gly Thr Leu Leu Arg Ala Phe Tyr Cys Ile Leu Glu Pro Arg Ser Gly 
            180                 185                 190         
Asn His Cys Pro Ala Gly Asn Ser Tyr Thr Ser Phe Ala Thr Tyr His 
        195                 200                 205             
Thr Pro Ala Thr Asp Cys Ser Asp Gly Asn Tyr Asn Arg Asn Ala Ser 
    210                 215                 220                 
Leu Asn Ser Phe Lys Glu Tyr Phe Asn Leu Arg Asn Cys Thr Phe Met 
225                 230                 235                 240 
Tyr Thr Tyr Asn Ile Thr Glu Asp Glu Ile Leu Glu Trp Phe Gly Ile 
                245                 250                 255     
Thr Gln Thr Ala Gln Gly Val His Leu Phe Ser Ser Arg Tyr Val Asp 
            260                 265                 270         
Leu Tyr Gly Gly Asn Met Phe Gln Phe Ala Thr Leu Pro Val Tyr Asp 
        275                 280                 285             
Thr Ile Lys Tyr Tyr Ser Ile Ile Pro His Ser Ile Arg Ser Ile Gln 
    290                 295                 300                 
Ser Asp Arg Lys Ala Trp Ala Ala Phe Tyr Val Tyr Lys Leu Gln Pro 
305                 310                 315                 320 
Leu Thr Phe Leu Leu Asp Phe Ser Val Asp Gly Tyr Ile Arg Arg Ala 
                325                 330                 335     
Ile Asp Cys Gly Phe Asn Asp Leu Ser Gln Leu His Cys Ser Tyr Glu 
            340                 345                 350         
Ser Phe Asp Val Glu Ser Gly Val Tyr Ser Val Ser Ser Phe Glu Ala 
        355                 360                 365             
Lys Pro Ser Gly Ser Val Val Glu Gln Ala Glu Gly Val Glu Cys Asp 
    370                 375                 380                 
Phe Ser Pro Leu Leu Ser Gly Thr Pro Pro Gln Val Tyr Asn Phe Lys 
385                 390                 395                 400 
Arg Leu Val Phe Thr Asn Cys Asn Tyr Asn Leu Thr Lys Leu Leu Ser 
                405                 410                 415     
Leu Phe Ser Val Asn Asp Phe Thr Cys Ser Gln Ile Ser Pro Ala Ala 
            420                 425                 430         
Ile Ala Ser Asn Cys Tyr Ser Ser Leu Ile Leu Asp Tyr Phe Ser Tyr 
        435                 440                 445             
Pro Leu Ser Met Lys Ser Asp Leu Ser Val Ser Ser Ala Gly Pro Ile 
    450                 455                 460                 
Ser Gln Phe Asn Tyr Lys Gln Ser Phe Ser Asn Pro Thr Cys Leu Ile 
465                 470                 475                 480 
Leu Ala Thr Val Pro His Asn Leu Thr Thr Ile Thr Lys Pro Leu Lys 
                485                 490                 495     
Tyr Ser Tyr Ile Asn Lys Cys Ser Arg Leu Leu Ser Asp Asp Arg Thr 
            500                 505                 510         
Glu Val Pro Gln Leu Val Asn Ala Asn Gln Tyr Ser Pro Cys Val Ser 
        515                 520                 525             
Ile Val Pro Ser Thr Val Trp Glu Asp Gly Asp Tyr Tyr Arg Lys Gln 
    530                 535                 540                 
Leu Ser Pro Leu Glu Gly Gly Gly Trp Leu Val Ala Ser Gly Ser Thr 
545                 550                 555                 560 
Val Ala Met Thr Glu Gln Leu Gln Met Gly Phe Gly Ile Thr Val Gln 
                565                 570                 575     
Tyr Gly Thr Asp Thr Asn Ser Val Cys Pro Lys Leu Glu Phe Ala Asn 
            580                 585                 590         
Asp Thr Lys Ile Ala Ser Gln Leu Gly Asn Cys Val Glu Tyr Ser Leu 
        595                 600                 605             
Tyr Gly Val Ser Gly Arg Gly Val Phe Gln Asn Cys Thr Ala Val Gly 
    610                 615                 620                 
Val Arg Gln Gln Arg Phe Val Tyr Asp Ala Tyr Gln Asn Leu Val Gly 
625                 630                 635                 640 
Tyr Tyr Ser Asp Asp Gly Asn Tyr Tyr Cys Leu Arg Ala Cys Val Ser 
                645                 650                 655     
Val Pro Val Ser Val Ile Tyr Asp Lys Glu Thr Lys Thr His Ala Thr 
            660                 665                 670         
Leu Phe Gly Ser Val Ala Cys Glu His Ile Ser Ser Thr Met Ser Gln 
        675                 680                 685             
Tyr Ser Arg Ser Thr Arg Ser Met Leu Lys Arg Arg Asp Ser Thr Tyr 
    690                 695                 700                 
Gly Pro Leu Gln Thr Pro Val Gly Cys Val Leu Gly Leu Val Asn Ser 
705                 710                 715                 720 
Ser Leu Phe Val Glu Asp Cys Lys Leu Pro Leu Gly Gln Ser Leu Cys 
                725                 730                 735     
Ala Leu Pro Asp Thr Pro Ser Thr Leu Thr Pro Arg Ser Val Arg Ser 
            740                 745                 750         
Val Pro Gly Glu Met Arg Leu Ala Ser Ile Ala Phe Asn His Pro Ile 
        755                 760                 765             
Gln Val Asp Gln Leu Asn Ser Ser Tyr Phe Lys Leu Ser Ile Pro Thr 
    770                 775                 780                 
Asn Phe Ser Phe Gly Val Thr Gln Glu Tyr Ile Gln Thr Thr Ile Gln 
785                 790                 795                 800 
Lys Val Thr Val Asp Cys Lys Gln Tyr Val Cys Asn Gly Phe Gln Lys 
                805                 810                 815     
Cys Glu Gln Leu Leu Arg Glu Tyr Gly Gln Phe Cys Ser Lys Ile Asn 
            820                 825                 830         
Gln Ala Leu His Gly Ala Asn Leu Arg Gln Asp Asp Ser Val Arg Asn 
        835                 840                 845             
Leu Phe Ala Ser Val Lys Ser Ser Gln Ser Ser Pro Ile Ile Pro Gly 
    850                 855                 860                 
Phe Gly Gly Asp Phe Asn Leu Thr Leu Leu Glu Pro Val Ser Ile Ser 
865                 870                 875                 880 
Thr Gly Ser Arg Ser Ala Arg Ser Ala Ile Glu Asp Leu Leu Phe Asp 
                885                 890                 895     
Lys Val Thr Ile Ala Asp Pro Gly Tyr Met Gln Gly Tyr Asp Asp Cys 
            900                 905                 910         
Met Gln Gln Gly Pro Ala Ser Ala Arg Asp Leu Ile Cys Ala Gln Tyr 
        915                 920                 925             
Val Ala Gly Tyr Lys Val Leu Pro Pro Leu Met Asp Val Asn Met Glu 
    930                 935                 940                 
Ala Ala Tyr Thr Ser Ser Leu Leu Gly Ser Ile Ala Gly Val Gly Trp 
945                 950                 955                 960 
Thr Ala Gly Leu Ser Ser Phe Ala Ala Ile Pro Phe Ala Gln Ser Ile 
                965                 970                 975     
Phe Tyr Arg Leu Asn Gly Val Gly Ile Thr Gln Gln Val Leu Ser Glu 
            980                 985                 990         
Asn Gln Lys Leu Ile Ala Asn Lys Phe Asn Gln Ala Leu Gly Ala Met 
        995                 1000                1005            
Gln Thr Gly Phe Thr Thr Thr Asn Glu Ala Phe Gln Lys Val Gln Asp 
    1010                1015                1020                
Ala Val Asn Asn Asn Ala Gln Ala Leu Ser Lys Leu Ala Ser Glu Leu 
1025                1030                1035                1040
Ser Asn Thr Phe Gly Ala Ile Ser Ala Ser Ile Gly Asp Ile Ile Gln 
                1045                1050                1055    
Arg Leu Asp Val Leu Glu Gln Asp Ala Gln Ile Asp Arg Leu Ile Asn 
            1060                1065                1070        
Gly Arg Leu Thr Thr Leu Asn Ala Phe Val Ala Gln Gln Leu Val Arg 
        1075                1080                1085            
Ser Glu Ser Ala Ala Leu Ser Ala Gln Leu Ala Lys Asp Lys Val Asn 
    1090                1095                1100                
Glu Cys Val Lys Ala Gln Ser Lys Arg Ser Gly Phe Cys Gly Gln Gly 
1105                1110                1115                1120
Thr His Ile Val Ser Phe Val Val Asn Ala Pro Asn Gly Leu Tyr Phe 
                1125                1130                1135    
Met His Val Gly Tyr Tyr Pro Ser Asn His Ile Glu Val Val Ser Ala 
            1140                1145                1150        
Tyr Gly Leu Cys Asp Ala Ala Asn Pro Thr Asn Cys Ile Ala Pro Val 
        1155                1160                1165            
Asn Gly Tyr Phe Ile Lys Thr Asn Asn Thr Arg Ile Val Asp Glu Trp 
    1170                1175                1180                
Ser Tyr Thr Gly Ser Ser Phe Tyr Ala Pro Glu Pro Ile Thr Ser Leu 
1185                1190                1195                1200
Asn Thr Lys Tyr Val Ala Pro Gln Val Thr Tyr Gln Asn Ile Ser Thr 
                1205                1210                1215    
Asn Leu Pro Pro Pro Leu Leu Gly Asn Ser Thr Gly Ile Asp Phe Gln 
            1220                1225                1230        
Asp Glu Leu Asp Glu Phe Phe Lys Asn Val Ser Thr Ser Ile Pro Asn 
        1235                1240                1245            
Phe Gly Ser Leu Thr Gln Ile Asn Thr Thr Leu Leu Asp Leu Thr Tyr 
    1250                1255                1260                
Glu Met Leu Ser Leu Gln Gln Val Val Lys Ala Leu Asn Glu Ser Tyr 
1265                1270                1275                1280
Ile Asp Leu Lys Glu Leu Gly Asn Tyr Thr Tyr Tyr Asn Lys Trp Pro 
                1285                1290                1295    
Trp Tyr Ile Trp Leu Gly Phe Ile Ala Gly Leu Val Ala Leu Ala Leu 
            1300                1305                1310        
Cys Val Phe Phe Ile Leu Cys Cys Thr Gly Cys Gly Thr Asn Cys Met 
        1315                1320                1325            
Gly Lys Leu Lys Cys Asn Arg Cys Cys Asp Arg Tyr Glu Glu Tyr Asp 
    1330                1335                1340                
Leu Glu Pro His Lys Val His Val His 
1345                1350            

<210> 4
<211> 4062
<212> DNA
<213> Human betacoronavirus 2c EMC/2012

<220> 
<221> source
<222> 1..4062
<223> /mol_type="unassigned DNA"
      /note="S protein"
      /organism="Human betacoronavirus 2c EMC/2012"

<400> 4
atgatacact cagtgtttct actgatgttc ttgttaacac ctacagaaag ttacgttgat       60

gtagggccag attctgttaa gtctgcttgt attgaggttg atatacaaca gactttcttt      120

gataaaactt ggcctaggcc aattgatgtt tctaaggctg acggtattat ataccctcaa      180

ggccgtacat attctaacat aactatcact tatcaaggtc tttttcccta tcagggagac      240

catggtgata tgtatgttta ctctgcagga catgctacag gcacaactcc acaaaagttg      300

tttgtagcta actattctca ggacgtcaaa cagtttgcta atgggtttgt cgtccgtata      360

ggagcagctg ccaattccac tggcactgtt attattagcc catctaccag cgctactata      420

cgaaaaattt accctgcttt tatgctgggt tcttcagttg gtaatttctc agatggtaaa      480

atgggccgct tcttcaatca tactctagtt cttttgcccg atggatgtgg cactttactt      540

agagcttttt attgtattct agagcctcgc tctggaaatc attgtcctgc tggcaattcc      600

tatacttctt ttgccactta tcacactcct gcaacagatt gttctgatgg caattacaat      660

cgtaatgcca gtctgaactc ttttaaggag tattttaatt tacgtaactg cacctttatg      720

tacacttata acattaccga agatgagatt ttagagtggt ttggcattac acaaactgct      780

caaggtgttc acctcttctc atctcggtat gttgatttgt acggcggcaa tatgtttcaa      840

tttgccacct tgcctgttta tgatactatt aagtattatt ctatcattcc tcacagtatt      900

cgttctatcc aaagtgatag aaaagcttgg gctgccttct acgtatataa acttcaaccg      960

ttaactttcc tgttggattt ttctgttgat ggttatatac gcagagctat agactgtggt     1020

tttaatgatt tgtcacaact ccactgctca tatgaatcct tcgatgttga atctggagtt     1080

tattcagttt cgtctttcga agcaaaacct tctggctcag ttgtggaaca ggctgaaggt     1140

gttgaatgtg atttttcacc tcttctgtct ggcacacctc ctcaggttta taatttcaag     1200

cgtttggttt ttaccaattg caattataat cttaccaaat tgctttcact tttttctgtg     1260

aatgatttta cttgtagtca aatatctcca gcagcaattg ctagcaactg ttattcttca     1320

ctgattttgg attacttttc atacccactt agtatgaaat ccgatctcag tgttagttct     1380

gctggtccaa tatcccagtt taattataaa cagtcctttt ctaatcccac atgtttgatt     1440

ttagcgactg ttcctcataa ccttactact attactaagc ctcttaagta cagctatatt     1500

aacaagtgct ctcgtcttct ttctgatgat cgtactgaag tacctcagtt agtgaacgct     1560

aatcaatact caccctgtgt atccattgtc ccatccactg tgtgggaaga cggtgattat     1620

tataggaaac aactatctcc acttgaaggt ggtggctggc ttgttgctag tggctcaact     1680

gttgccatga ctgagcaatt acagatgggc tttggtatta cagttcaata tggtacagac     1740

accaatagtg tttgccccaa gcttgaattt gctaatgaca caaaaattgc ctctcaatta     1800

ggcaattgcg tggaatattc cctctatggt gtttcgggcc gtggtgtttt tcagaattgc     1860

acagctgtag gtgttcgaca gcagcgcttt gtttatgatg cgtaccagaa tttagttggc     1920

tattattctg atgatggcaa ctactactgt ttgcgtgctt gtgttagtgt tcctgtttct     1980

gtcatctatg ataaagaaac taaaacccac gctactctat ttggtagtgt tgcatgtgaa     2040

cacatttctt ctaccatgtc tcaatactcc cgttctacgc gatcaatgct taaacggcga     2100

gattctacat atggccccct tcagacacct gttggttgtg tcctaggact tgttaattcc     2160

tctttgttcg tagaggactg caagttgcct cttggtcaat ctctctgtgc tcttcctgac     2220

acacctagta ctctcacacc tcgcagtgtg cgctctgttc caggtgaaat gcgcttggca     2280

tccattgctt ttaatcatcc tattcaggtt gatcaactta atagtagtta ttttaaatta     2340

agtataccca ctaatttttc ctttggtgtg actcaggagt acattcagac aaccattcag     2400

aaagttactg ttgattgtaa acagtacgtt tgcaatggtt tccagaagtg tgagcaatta     2460

ctgcgcgagt atggccagtt ttgttccaaa ataaaccagg ctctccatgg tgccaattta     2520

cgccaggatg attctgtacg taatttgttt gcgagcgtga aaagctctca atcatctcct     2580

atcataccag gttttggagg tgactttaat ttgacacttc tagaacctgt ttctatatct     2640

actggcagtc gtagtgcacg tagtgctatt gaggatttgc tatttgacaa agtcactata     2700

gctgatcctg gttatatgca aggttacgat gattgcatgc agcaaggtcc agcatcagct     2760

cgtgatctta tttgtgctca atatgtggct ggttacaaag tattacctcc tcttatggat     2820

gttaatatgg aagccgcgta tacttcatct ttgcttggca gcatagcagg tgttggctgg     2880

actgctggct tatcctcctt tgctgctatt ccatttgcac agagtatctt ttataggtta     2940

aacggtgttg gcattactca acaggttctt tcagagaacc aaaagcttat tgccaataag     3000

tttaatcagg ctctgggagc tatgcaaaca ggcttcacta caactaatga agcttttcag     3060

aaggttcagg atgctgtgaa caacaatgca caggctctat ccaaattagc tagcgagcta     3120

tctaatactt ttggtgctat ttccgcctct attggagaca tcatacaacg tcttgatgtt     3180

ctcgaacagg acgcccaaat agacagactt attaatggcc gtttgacaac actaaatgct     3240

tttgttgcac agcagcttgt tcgttccgaa tcagctgctc tttccgctca attggctaaa     3300

gataaagtca atgagtgtgt caaggcacaa tccaagcgtt ctggattttg cggtcaaggc     3360

acacatatag tgtcctttgt tgtaaatgcc cctaatggcc tttacttcat gcatgttggt     3420

tattacccta gcaaccacat tgaggttgtt tctgcttatg gtctttgcga tgcagctaac     3480

cctactaatt gtatagcccc tgttaatggc tactttatta aaactaataa cactaggatt     3540

gttgatgagt ggtcatatac tggctcgtcc ttctatgcac ctgagcccat tacctccctt     3600

aatactaagt atgttgcacc acaggtgaca taccaaaaca tttctactaa cctccctcct     3660

cctcttctcg gcaattccac cgggattgac ttccaagatg agttggatga gtttttcaaa     3720

aatgttagca ccagtatacc taattttggt tccctaacac agattaatac tacattactc     3780

gatcttacct acgagatgtt gtctcttcaa caagttgtta aagcccttaa tgagtcttac     3840

atagacctta aagagcttgg caattatact tattacaaca aatggccgtg gtacatttgg     3900

cttggtttca ttgctgggct tgttgcctta gctctatgcg tcttcttcat actgtgctgc     3960

actggttgtg gcacaaactg tatgggaaaa cttaagtgta atcgttgttg tgatagatac     4020

gaggaatacg acctcgagcc gcataaggtt catgttcact aa                        4062


<210> 5
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..25
<223> /mol_type="unassigned DNA"
      /note="MVA_Del3-F primer"
      /organism="Artificial Sequence"

<400> 5
gatgagtgta gatgctgtta ttttg                                          25


<210> 6
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..24
<223> /mol_type="unassigned DNA"
      /note="MVA_Del3-R primer"
      /organism="Artificial Sequence"

<400> 6
gcagctaaaa gaataatgga attg                                           24


<210> 7
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..19
<223> /mol_type="unassigned DNA"
      /note="MVA_069R primer"
      /organism="Artificial Sequence"

<400> 7
attctcgctg aggagttgg                                                 19


<210> 8
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..19
<223> /mol_type="unassigned DNA"
      /note="MVA_070L primer"
      /organism="Artificial Sequence"

<400> 8
gtcgtgtcta caaaaggag                                                 19


<210> 9
<211> 82
<212> PRT
<213> Human betacoronavirus 2c EMC/2012

<220> 
<223> E protein

<400> 9
Met Leu Pro Phe Val Gln Glu Arg Ile Gly Leu Phe Ile Val Asn Phe 
1               5                   10                  15      
Phe Ile Phe Thr Val Val Cys Ala Ile Thr Leu Leu Val Cys Met Ala 
            20                  25                  30          
Phe Leu Thr Ala Thr Arg Leu Cys Val Gln Cys Met Thr Gly Phe Asn 
        35                  40                  45              
Thr Leu Leu Val Gln Pro Ala Leu Tyr Leu Tyr Asn Thr Gly Arg Ser 
    50                  55                  60                  
Val Tyr Val Lys Phe Gln Asp Ser Lys Pro Pro Leu Pro Pro Asp Glu 
65                  70                  75                  80  
Trp Val 
        

<210> 10
<211> 219
<212> PRT
<213> Human betacoronavirus 2c EMC/2012

<220> 
<223> M protein

<400> 10
Met Ser Asn Met Thr Gln Leu Thr Glu Ala Gln Ile Ile Ala Ile Ile 
1               5                   10                  15      
Lys Asp Trp Asn Phe Ala Trp Ser Leu Ile Phe Leu Leu Ile Thr Ile 
            20                  25                  30          
Val Leu Gln Tyr Gly Tyr Pro Ser Arg Ser Met Thr Val Tyr Val Phe 
        35                  40                  45              
Lys Met Phe Val Leu Trp Leu Leu Trp Pro Ser Ser Met Ala Leu Ser 
    50                  55                  60                  
Ile Phe Ser Ala Val Tyr Pro Ile Asp Leu Ala Ser Gln Ile Ile Ser 
65                  70                  75                  80  
Gly Ile Val Ala Ala Val Ser Ala Met Met Trp Ile Ser Tyr Phe Val 
                85                  90                  95      
Gln Ser Ile Arg Leu Phe Met Arg Thr Gly Ser Trp Trp Ser Phe Asn 
            100                 105                 110         
Pro Glu Thr Asn Cys Leu Leu Asn Val Pro Phe Gly Gly Thr Thr Val 
        115                 120                 125             
Val Arg Pro Leu Val Glu Asp Ser Thr Ser Val Thr Ala Val Val Thr 
    130                 135                 140                 
Asn Gly His Leu Lys Met Ala Gly Met His Phe Gly Ala Cys Asp Tyr 
145                 150                 155                 160 
Asp Arg Leu Pro Asn Glu Val Thr Val Ala Lys Pro Asn Val Leu Ile 
                165                 170                 175     
Ala Leu Lys Met Val Lys Arg Gln Ser Tyr Gly Thr Asn Ser Gly Val 
            180                 185                 190         
Ala Ile Tyr His Arg Tyr Lys Ala Gly Asn Tyr Arg Ser Pro Pro Ile 
        195                 200                 205             
Thr Ala Asp Ile Glu Leu Ala Leu Leu Arg Ala 
    210                 215                 

<210> 11
<211> 103
<212> PRT
<213> Human betacoronavirus 2c EMC/2012

<220> 
<223> protein 3

<400> 11
Met Arg Val Gln Arg Pro Pro Thr Leu Leu Leu Val Phe Ser Leu Ser 
1               5                   10                  15      
Leu Leu Val Thr Ala Ser Ser Lys Pro Leu Tyr Val Pro Glu His Cys 
            20                  25                  30          
Gln Asn Tyr Ser Gly Cys Met Leu Arg Ala Cys Ile Lys Thr Ala Gln 
        35                  40                  45              
Ala Asp Thr Ala Gly Leu Tyr Thr Asn Phe Arg Ile Asp Val Pro Ser 
    50                  55                  60                  
Ala Glu Ser Thr Gly Thr Gln Ser Val Ser Val Asp Leu Glu Ser Thr 
65                  70                  75                  80  
Ser Thr His Asp Gly Pro Thr Glu His Val Thr Ser Val Asn Leu Phe 
                85                  90                  95      
Asp Val Gly Tyr Ser Val Asn 
            100             

<210> 12
<211> 109
<212> PRT
<213> Human betacoronavirus 2c EMC/2012

<220> 
<223> protein 4a

<400> 12
Met Asp Tyr Val Ser Leu Leu Asn Gln Ile Trp Gln Lys Tyr Leu Asn 
1               5                   10                  15      
Ser Pro Tyr Thr Thr Cys Leu Tyr Ile Pro Lys Pro Thr Ala Lys Tyr 
            20                  25                  30          
Thr Pro Leu Val Gly Thr Ser Leu His Pro Val Leu Trp Asn Cys Gln 
        35                  40                  45              
Leu Ser Phe Ala Gly Tyr Thr Glu Ser Ala Val Asn Ser Thr Lys Ala 
    50                  55                  60                  
Leu Ala Lys Gln Asp Ala Ala Gln Arg Ile Ala Trp Leu Leu His Lys 
65                  70                  75                  80  
Asp Gly Gly Ile Pro Asp Gly Cys Ser Leu Tyr Leu Arg His Ser Ser 
                85                  90                  95      
Leu Phe Ala Gln Ser Glu Glu Glu Glu Pro Phe Ser Asn 
            100                 105                 

<210> 13
<211> 246
<212> PRT
<213> Human betacoronavirus 2c EMC/2012

<220> 
<223> protein 4b

<400> 13
Met Glu Glu Ser Leu Met Asp Val Pro Ser Thr Ser Gly Thr Gln Val 
1               5                   10                  15      
Tyr Ser Arg Lys Ala Arg Lys Arg Ser His Ser Pro Thr Lys Lys Leu 
            20                  25                  30          
Arg Tyr Val Lys Arg Arg Phe Ser Leu Leu Arg His Glu Asp Leu Ser 
        35                  40                  45              
Val Ile Val Gln Pro Thr His Tyr Val Arg Val Thr Phe Ser Asp Pro 
    50                  55                  60                  
Asn Met Trp Tyr Leu Arg Ser Gly His His Leu His Ser Val His Asn 
65                  70                  75                  80  
Trp Leu Lys Pro Tyr Gly Gly Gln Pro Val Ser Glu Tyr His Ile Thr 
                85                  90                  95      
Leu Ala Leu Leu Asn Leu Thr Asp Glu Asp Leu Ala Arg Asp Phe Ser 
            100                 105                 110         
Pro Ile Ala Leu Phe Leu Arg Asn Val Arg Phe Glu Leu His Glu Phe 
        115                 120                 125             
Ala Leu Leu Arg Lys Thr Leu Val Leu Asn Ala Ser Glu Ile Tyr Cys 
    130                 135                 140                 
Ala Asn Ile His Arg Phe Lys Pro Val Tyr Arg Val Asn Thr Ala Ile 
145                 150                 155                 160 
Pro Thr Ile Lys Asp Trp Leu Leu Val Gln Gly Phe Ser Leu Tyr His 
                165                 170                 175     
Ser Gly Leu Pro Leu His Met Ser Ile Ser Lys Leu His Ala Leu Asp 
            180                 185                 190         
Asp Val Thr Arg Asn Tyr Ile Ile Thr Met Pro Cys Phe Arg Thr Tyr 
        195                 200                 205             
Pro Gln Gln Met Phe Val Thr Pro Leu Ala Val Asp Val Val Ser Ile 
    210                 215                 220                 
Arg Ser Ser Asn Gln Gly Asn Lys Gln Ile Val His Ser Tyr Pro Ile 
225                 230                 235                 240 
Leu His His Pro Gly Phe 
                245     

<210> 14
<211> 224
<212> PRT
<213> Human betacoronavirus 2c EMC/2012

<220> 
<223> protein 5

<400> 14
Met Ala Phe Ser Ala Ser Leu Phe Lys Pro Val Gln Leu Val Pro Val 
1               5                   10                  15      
Ser Pro Ala Phe His Arg Ile Glu Ser Thr Asp Ser Ile Val Phe Thr 
            20                  25                  30          
Tyr Ile Pro Ala Ser Gly Tyr Val Ala Ala Leu Ala Val Asn Val Cys 
        35                  40                  45              
Leu Ile Pro Leu Leu Leu Leu Leu Arg Gln Asp Thr Cys Arg Arg Ser 
    50                  55                  60                  
Ile Ile Arg Thr Met Val Leu Tyr Phe Leu Val Leu Tyr Asn Phe Leu 
65                  70                  75                  80  
Leu Ala Ile Val Leu Val Asn Gly Val His Tyr Pro Thr Gly Ser Cys 
                85                  90                  95      
Leu Ile Ala Phe Leu Val Ile Leu Ile Ile Leu Trp Phe Val Asp Arg 
            100                 105                 110         
Ile Arg Phe Cys Leu Met Leu Asn Ser Tyr Ile Pro Leu Phe Asp Met 
        115                 120                 125             
Arg Ser His Phe Ile Arg Val Ser Thr Val Ser Ser His Gly Met Val 
    130                 135                 140                 
Pro Val Ile His Thr Lys Pro Leu Phe Ile Arg Asn Phe Asp Gln Arg 
145                 150                 155                 160 
Cys Ser Cys Ser Arg Cys Phe Tyr Leu His Ser Ser Thr Tyr Ile Glu 
                165                 170                 175     
Cys Thr Tyr Ile Ser Arg Phe Ser Lys Ile Ser Leu Val Ser Val Thr 
            180                 185                 190         
Asp Phe Ser Leu Asn Gly Asn Val Ser Thr Val Phe Val Pro Ala Thr 
        195                 200                 205             
Arg Asp Ser Val Pro Leu His Ile Ile Ala Pro Ser Ser Leu Ile Val 
    210                 215                 220                 



