                         SEQUENCE LISTING

<110>  The Johns Hopkins University
 
<120>  SYNTHETIC ENHANCEMENT OF THE T-CELL ARMAMENTARIUM AS AN 
       ANTI-CANCER THERAPY

<130>  P12863-02

<150>  62/104,368
<151>  2015-01-16

<160>  48    

<170>  PatentIn version 3.5

<210>  1
<211>  1410
<212>  DNA
<213>  Aeromonas hydrophila

<400>  1
gcagagcccg tctatccaga ccagcttcgc ttgttttcat tgggccaagg ggtctgtggc       60

gacaagtatc gccccgtcaa tcgagaagaa gcccaaagcg ttaaaagcaa tattgtcggc      120

atgatggggc aatggcaaat aagcgggctg gccaacggct gggtcattat ggggccgggt      180

tataacggtg aaataaaacc agggacagcg tccaatacct ggtgttatcc gaccaatcct      240

gttaccggtg aaataccgac actgtctgcc ctggatattc cagatggtga cgaagtcgat      300

gtgcagtggc gactggtaca tgacagtgcg aatttcatca aaccaaccag ctatctggcc      360

cattacctcg gttatgcctg ggtgggcggc aatcacagcc aatatgtcgg cgaagacatg      420

gatgtgaccc gtgatggcga cggctgggtg atccgtggca acaatgacgg cggctgtgac      480

ggctatcgct gtggtgacaa gacggccatc aaggtcagca acttcgccta taacctggat      540

cccgacagct tcaagcatgg cgatgtcacc cagtccgacc gccagctggt caagactgtg      600

gtgggctggg cggtcaacga cagcgacacc ccccaatccg gctatgacgt caccctgcgc      660

tacgacacag ccaccaactg gtccaagacc aacacctatg gcctgagcga gaaggtgacc      720

accaagaaca agttcaagtg gccactggtg ggggaaaccc aactctccat cgagattgct      780

gccaatcagt cctgggcgtc ccagaacggg ggctcgacca ccacctccct gtctcagtcc      840

gtgcgaccga ctgtgccggc ccgctccaag atcccggtga agatagagct ctacaaggcc      900

gacatctcct atccctatga gttcaaggcc gatgtcagct atgacctgac cctgagcggc      960

ttcctgcgct ggggcggcaa cgcctggtat acccacccgg acaaccgtcc gaactggaac     1020

cacaccttcg tcataggtcc gtacaaggac aaggcgagca gcattcggta ccagtgggac     1080

aagcgttaca tcccgggtga agtgaagtgg tgggactgga actggaccat acagcagaac     1140

ggtctgtcta ccatgcagaa caacctggcc agagtgctgc gcccggtgcg ggcggggatc     1200

accggtgatt tcagtgccga gagccagttt gccggcaaca tagagatcgg tgctcccgtg     1260

ccgctcgcgg ctgacagcaa ggtgcgtcgt gctcgcagtg tggacggcgc tggtcaaggc     1320

ctgaggctgg agatcccgct cgatgcgcaa gagctctccg ggcttggctt caacaacgtc     1380

agcctcagcg tgacccctgc tgccaatcaa                                      1410


<210>  2
<211>  470
<212>  PRT
<213>  Aeromonas hydrophila

<400>  2

Ala Glu Pro Val Tyr Pro Asp Gln Leu Arg Leu Phe Ser Leu Gly Gln 
1               5                   10                  15      


Gly Val Cys Gly Asp Lys Tyr Arg Pro Val Asn Arg Glu Glu Ala Gln 
            20                  25                  30          


Ser Val Lys Ser Asn Ile Val Gly Met Met Gly Gln Trp Gln Ile Ser 
        35                  40                  45              


Gly Leu Ala Asn Gly Trp Val Ile Met Gly Pro Gly Tyr Asn Gly Glu 
    50                  55                  60                  


Ile Lys Pro Gly Thr Ala Ser Asn Thr Trp Cys Tyr Pro Thr Asn Pro 
65                  70                  75                  80  


Val Thr Gly Glu Ile Pro Thr Leu Ser Ala Leu Asp Ile Pro Asp Gly 
                85                  90                  95      


Asp Glu Val Asp Val Gln Trp Arg Leu Val His Asp Ser Ala Asn Phe 
            100                 105                 110         


Ile Lys Pro Thr Ser Tyr Leu Ala His Tyr Leu Gly Tyr Ala Trp Val 
        115                 120                 125             


Gly Gly Asn His Ser Gln Tyr Val Gly Glu Asp Met Asp Val Thr Arg 
    130                 135                 140                 


Asp Gly Asp Gly Trp Val Ile Arg Gly Asn Asn Asp Gly Gly Cys Asp 
145                 150                 155                 160 


Gly Tyr Arg Cys Gly Asp Lys Thr Ala Ile Lys Val Ser Asn Phe Ala 
                165                 170                 175     


Tyr Asn Leu Asp Pro Asp Ser Phe Lys His Gly Asp Val Thr Gln Ser 
            180                 185                 190         


Asp Arg Gln Leu Val Lys Thr Val Val Gly Trp Ala Val Asn Asp Ser 
        195                 200                 205             


Asp Thr Pro Gln Ser Gly Tyr Asp Val Thr Leu Arg Tyr Asp Thr Ala 
    210                 215                 220                 


Thr Asn Trp Ser Lys Thr Asn Thr Tyr Gly Leu Ser Glu Lys Val Thr 
225                 230                 235                 240 


Thr Lys Asn Lys Phe Lys Trp Pro Leu Val Gly Glu Thr Gln Leu Ser 
                245                 250                 255     


Ile Glu Ile Ala Ala Asn Gln Ser Trp Ala Ser Gln Asn Gly Gly Ser 
            260                 265                 270         


Thr Thr Thr Ser Leu Ser Gln Ser Val Arg Pro Thr Val Pro Ala Arg 
        275                 280                 285             


Ser Lys Ile Pro Val Lys Ile Glu Leu Tyr Lys Ala Asp Ile Ser Tyr 
    290                 295                 300                 


Pro Tyr Glu Phe Lys Ala Asp Val Ser Tyr Asp Leu Thr Leu Ser Gly 
305                 310                 315                 320 


Phe Leu Arg Trp Gly Gly Asn Ala Trp Tyr Thr His Pro Asp Asn Arg 
                325                 330                 335     


Pro Asn Trp Asn His Thr Phe Val Ile Gly Pro Tyr Lys Asp Lys Ala 
            340                 345                 350         


Ser Ser Ile Arg Tyr Gln Trp Asp Lys Arg Tyr Ile Pro Gly Glu Val 
        355                 360                 365             


Lys Trp Trp Asp Trp Asn Trp Thr Ile Gln Gln Asn Gly Leu Ser Thr 
    370                 375                 380                 


Met Gln Asn Asn Leu Ala Arg Val Leu Arg Pro Val Arg Ala Gly Ile 
385                 390                 395                 400 


Thr Gly Asp Phe Ser Ala Glu Ser Gln Phe Ala Gly Asn Ile Glu Ile 
                405                 410                 415     


Gly Ala Pro Val Pro Leu Ala Ala Asp Ser Lys Val Arg Arg Ala Arg 
            420                 425                 430         


Ser Val Asp Gly Ala Gly Gln Gly Leu Arg Leu Glu Ile Pro Leu Asp 
        435                 440                 445             


Ala Gln Glu Leu Ser Gly Leu Gly Phe Asn Asn Val Ser Leu Ser Val 
    450                 455                 460                 


Thr Pro Ala Ala Asn Gln 
465                 470 


<210>  3
<211>  1410
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Proaerolysin with a PSA sequence substituted for the furin site.

<400>  3
gcagagcccg tctatccaga ccagcttcgc ttgttttcat tgggccaagg ggtctgtggc       60

gacaagtatc gccccgtcaa tcgagaagaa gcccaaagcg ttaaaagcaa tattgtcggc      120

atgatggggc aatggcaaat aagcgggctg gccaacggct gggtcattat ggggccgggt      180

tataacggtg aaataaaacc agggacagcg tccaatacct ggtgttatcc gaccaatcct      240

gttaccggtg aaataccgac actgtctgcc ctggatattc cagatggtga cgaagtcgat      300

gtgcagtggc gactggtaca tgacagtgcg aatttcatca aaccaaccag ctatctggcc      360

cattacctcg gttatgcctg ggtgggcggc aatcacagcc aatatgtcgg cgaagacatg      420

gatgtgaccc gtgatggcga cggctgggtg atccgtggca acaatgacgg cggctgtgac      480

ggctatcgct gtggtgacaa gacggccatc aaggtcagca acttcgccta taacctggat      540

cccgacagct tcaagcatgg cgatgtcacc cagtccgacc gccagctggt caagactgtg      600

gtgggctggg cggtcaacga cagcgacacc ccccaatccg gctatgacgt caccctgcgc      660

tacgacacag ccaccaactg gtccaagacc aacacctatg gcctgagcga gaaggtgacc      720

accaagaaca agttcaagtg gccactggtg ggggaaaccc aactctccat cgagattgct      780

gccaatcagt cctgggcgtc ccagaacggg ggctcgacca ccacctccct gtctcagtcc      840

gtgcgaccga ctgtgccggc ccgctccaag atcccggtga agatagagct ctacaaggcc      900

gacatctcct atccctatga gttcaaggcc gatgtcagct atgacctgac cctgagcggc      960

ttcctgcgct ggggcggcaa cgcctggtat acccacccgg acaaccgtcc gaactggaac     1020

cacaccttcg tcataggtcc gtacaaggac aaggcgagca gcattcggta ccagtgggac     1080

aagcgttaca tcccgggtga agtgaagtgg tgggactgga actggaccat acagcagaac     1140

ggtctgtcta ccatgcagaa caacctggcc agagtgctgc gcccggtgcg ggcggggatc     1200

accggtgatt tcagtgccga gagccagttt gccggcaaca tagagatcgg tgctcccgtg     1260

ccgctcgcgg ctgacagcca ttcctccaag ctgcagagtg tggacggcgc tggtcaaggc     1320

ctgaggctgg agatcccgct cgatgcgcaa gagctctccg ggcttggctt caacaacgtc     1380

agcctcagcg tgacccctgc tgccaatcaa                                      1410


<210>  4
<211>  470
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Proaerolysin with a PSA sequence substituted for the furin site.

<400>  4

Ala Glu Pro Val Tyr Pro Asp Gln Leu Arg Leu Phe Ser Leu Gly Gln 
1               5                   10                  15      


Gly Val Cys Gly Asp Lys Tyr Arg Pro Val Asn Arg Glu Glu Ala Gln 
            20                  25                  30          


Ser Val Lys Ser Asn Ile Val Gly Met Met Gly Gln Trp Gln Ile Ser 
        35                  40                  45              


Gly Leu Ala Asn Gly Trp Val Ile Met Gly Pro Gly Tyr Asn Gly Glu 
    50                  55                  60                  


Ile Lys Pro Gly Thr Ala Ser Asn Thr Trp Cys Tyr Pro Thr Asn Pro 
65                  70                  75                  80  


Val Thr Gly Glu Ile Pro Thr Leu Ser Ala Leu Asp Ile Pro Asp Gly 
                85                  90                  95      


Asp Glu Val Asp Val Gln Trp Arg Leu Val His Asp Ser Ala Asn Phe 
            100                 105                 110         


Ile Lys Pro Thr Ser Tyr Leu Ala His Tyr Leu Gly Tyr Ala Trp Val 
        115                 120                 125             


Gly Gly Asn His Ser Gln Tyr Val Gly Glu Asp Met Asp Val Thr Arg 
    130                 135                 140                 


Asp Gly Asp Gly Trp Val Ile Arg Gly Asn Asn Asp Gly Gly Cys Asp 
145                 150                 155                 160 


Gly Tyr Arg Cys Gly Asp Lys Thr Ala Ile Lys Val Ser Asn Phe Ala 
                165                 170                 175     


Tyr Asn Leu Asp Pro Asp Ser Phe Lys His Gly Asp Val Thr Gln Ser 
            180                 185                 190         


Asp Arg Gln Leu Val Lys Thr Val Val Gly Trp Ala Val Asn Asp Ser 
        195                 200                 205             


Asp Thr Pro Gln Ser Gly Tyr Asp Val Thr Leu Arg Tyr Asp Thr Ala 
    210                 215                 220                 


Thr Asn Trp Ser Lys Thr Asn Thr Tyr Gly Leu Ser Glu Lys Val Thr 
225                 230                 235                 240 


Thr Lys Asn Lys Phe Lys Trp Pro Leu Val Gly Glu Thr Gln Leu Ser 
                245                 250                 255     


Ile Glu Ile Ala Ala Asn Gln Ser Trp Ala Ser Gln Asn Gly Gly Ser 
            260                 265                 270         


Thr Thr Thr Ser Leu Ser Gln Ser Val Arg Pro Thr Val Pro Ala Arg 
        275                 280                 285             


Ser Lys Ile Pro Val Lys Ile Glu Leu Tyr Lys Ala Asp Ile Ser Tyr 
    290                 295                 300                 


Pro Tyr Glu Phe Lys Ala Asp Val Ser Tyr Asp Leu Thr Leu Ser Gly 
305                 310                 315                 320 


Phe Leu Arg Trp Gly Gly Asn Ala Trp Tyr Thr His Pro Asp Asn Arg 
                325                 330                 335     


Pro Asn Trp Asn His Thr Phe Val Ile Gly Pro Tyr Lys Asp Lys Ala 
            340                 345                 350         


Ser Ser Ile Arg Tyr Gln Trp Asp Lys Arg Tyr Ile Pro Gly Glu Val 
        355                 360                 365             


Lys Trp Trp Asp Trp Asn Trp Thr Ile Gln Gln Asn Gly Leu Ser Thr 
    370                 375                 380                 


Met Gln Asn Asn Leu Ala Arg Val Leu Arg Pro Val Arg Ala Gly Ile 
385                 390                 395                 400 


Thr Gly Asp Phe Ser Ala Glu Ser Gln Phe Ala Gly Asn Ile Glu Ile 
                405                 410                 415     


Gly Ala Pro Val Pro Leu Ala Ala Asp Ser His Ser Ser Lys Leu Gln 
            420                 425                 430         


Ser Val Asp Gly Ala Gly Gln Gly Leu Arg Leu Glu Ile Pro Leu Asp 
        435                 440                 445             


Ala Gln Glu Leu Ser Gly Leu Gly Phe Asn Asn Val Ser Leu Ser Val 
    450                 455                 460                 


Thr Pro Ala Ala Asn Gln 
465                 470 


<210>  5
<211>  6
<212>  PRT
<213>  Homo sapiens

<400>  5

His Ser Ser Lys Leu Gln 
1               5       


<210>  6
<211>  1410
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Proaerolysin with a PSA sequence substituted for the furin site.

<400>  6
gcagagcccg tctatccaga ccagcttcgc ttgttttcat tgggccaagg ggtctgtggc       60

gacaagtatc gccccgtcaa tcgagaagaa gcccaaagcg ttaaaagcaa tattgtcggc      120

atgatggggc aatggcaaat aagcgggctg gccaacggct gggtcattat ggggccgggt      180

tataacggtg aaataaaacc agggacagcg tccaatacct ggtgttatcc gaccaatcct      240

gttaccggtg aaataccgac actgtctgcc ctggatattc cagatggtga cgaagtcgat      300

gtgcagtggc gactggtaca tgacagtgcg aatttcatca aaccaaccag ctatctggcc      360

cattacctcg gttatgcctg ggtgggcggc aatcacagcc aatatgtcgg cgaagacatg      420

gatgtgaccc gtgatggcga cggctgggtg atccgtggca acaatgacgg cggctgtgac      480

ggctatcgct gtggtgacaa gacggccatc aaggtcagca acttcgccta taacctggat      540

cccgacagct tcaagcatgg cgatgtcacc cagtccgacc gccagctggt caagactgtg      600

gtgggctggg cggtcaacga cagcgacacc ccccaatccg gctatgacgt caccctgcgc      660

tacgacacag ccaccaactg gtccaagacc aacacctatg gcctgagcga gaaggtgacc      720

accaagaaca agttcaagtg gccactggtg ggggaaaccc aactctccat cgagattgct      780

gccaatcagt cctgggcgtc ccagaacggg ggctcgacca ccacctccct gtctcagtcc      840

gtgcgaccga ctgtgccggc ccgctccaag atcccggtga agatagagct ctacaaggcc      900

gacatctcct atccctatga gttcaaggcc gatgtcagct atgacctgac cctgagcggc      960

ttcctgcgct ggggcggcaa cgcctggtat acccacccgg acaaccgtcc gaactggaac     1020

cacaccttcg tcataggtcc gtacaaggac aaggcgagca gcattcggta ccagtgggac     1080

aagcgttaca tcccgggtga agtgaagtgg tgggactgga actggaccat acagcagaac     1140

ggtctgtcta ccatgcagaa caacctggcc agagtgctgc gcccggtgcg ggcggggatc     1200

accggtgatt tcagtgccga gagccagttt gccggcaaca tagagatcgg tgctcccgtg     1260

ccgctcgcgg ctgacagcca ttcctccaag ctgcagagtg ccgacggcgc tggtcaaggc     1320

ctgaggctgg agatcccgct cgatgcgcaa gagctctccg ggcttggctt caacaacgtc     1380

agcctcagcg tgacccctgc tgccaatcaa                                      1410


<210>  7
<211>  470
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Proaerolysin with a PSA sequence substituted for the furin site.

<400>  7

Ala Glu Pro Val Tyr Pro Asp Gln Leu Arg Leu Phe Ser Leu Gly Gln 
1               5                   10                  15      


Gly Val Cys Gly Asp Lys Tyr Arg Pro Val Asn Arg Glu Glu Ala Gln 
            20                  25                  30          


Ser Val Lys Ser Asn Ile Val Gly Met Met Gly Gln Trp Gln Ile Ser 
        35                  40                  45              


Gly Leu Ala Asn Gly Trp Val Ile Met Gly Pro Gly Tyr Asn Gly Glu 
    50                  55                  60                  


Ile Lys Pro Gly Thr Ala Ser Asn Thr Trp Cys Tyr Pro Thr Asn Pro 
65                  70                  75                  80  


Val Thr Gly Glu Ile Pro Thr Leu Ser Ala Leu Asp Ile Pro Asp Gly 
                85                  90                  95      


Asp Glu Val Asp Val Gln Trp Arg Leu Val His Asp Ser Ala Asn Phe 
            100                 105                 110         


Ile Lys Pro Thr Ser Tyr Leu Ala His Tyr Leu Gly Tyr Ala Trp Val 
        115                 120                 125             


Gly Gly Asn His Ser Gln Tyr Val Gly Glu Asp Met Asp Val Thr Arg 
    130                 135                 140                 


Asp Gly Asp Gly Trp Val Ile Arg Gly Asn Asn Asp Gly Gly Cys Asp 
145                 150                 155                 160 


Gly Tyr Arg Cys Gly Asp Lys Thr Ala Ile Lys Val Ser Asn Phe Ala 
                165                 170                 175     


Tyr Asn Leu Asp Pro Asp Ser Phe Lys His Gly Asp Val Thr Gln Ser 
            180                 185                 190         


Asp Arg Gln Leu Val Lys Thr Val Val Gly Trp Ala Val Asn Asp Ser 
        195                 200                 205             


Asp Thr Pro Gln Ser Gly Tyr Asp Val Thr Leu Arg Tyr Asp Thr Ala 
    210                 215                 220                 


Thr Asn Trp Ser Lys Thr Asn Thr Tyr Gly Leu Ser Glu Lys Val Thr 
225                 230                 235                 240 


Thr Lys Asn Lys Phe Lys Trp Pro Leu Val Gly Glu Thr Gln Leu Ser 
                245                 250                 255     


Ile Glu Ile Ala Ala Asn Gln Ser Trp Ala Ser Gln Asn Gly Gly Ser 
            260                 265                 270         


Thr Thr Thr Ser Leu Ser Gln Ser Val Arg Pro Thr Val Pro Ala Arg 
        275                 280                 285             


Ser Lys Ile Pro Val Lys Ile Glu Leu Tyr Lys Ala Asp Ile Ser Tyr 
    290                 295                 300                 


Pro Tyr Glu Phe Lys Ala Asp Val Ser Tyr Asp Leu Thr Leu Ser Gly 
305                 310                 315                 320 


Phe Leu Arg Trp Gly Gly Asn Ala Trp Tyr Thr His Pro Asp Asn Arg 
                325                 330                 335     


Pro Asn Trp Asn His Thr Phe Val Ile Gly Pro Tyr Lys Asp Lys Ala 
            340                 345                 350         


Ser Ser Ile Arg Tyr Gln Trp Asp Lys Arg Tyr Ile Pro Gly Glu Val 
        355                 360                 365             


Lys Trp Trp Asp Trp Asn Trp Thr Ile Gln Gln Asn Gly Leu Ser Thr 
    370                 375                 380                 


Met Gln Asn Asn Leu Ala Arg Val Leu Arg Pro Val Arg Ala Gly Ile 
385                 390                 395                 400 


Thr Gly Asp Phe Ser Ala Glu Ser Gln Phe Ala Gly Asn Ile Glu Ile 
                405                 410                 415     


Gly Ala Pro Val Pro Leu Ala Ala Asp Ser His Ser Ser Lys Leu Gln 
            420                 425                 430         


Ser Ala Asp Gly Ala Gly Gln Gly Leu Arg Leu Glu Ile Pro Leu Asp 
        435                 440                 445             


Ala Gln Glu Leu Ser Gly Leu Gly Phe Asn Asn Val Ser Leu Ser Val 
    450                 455                 460                 


Thr Pro Ala Ala Asn Gln 
465                 470 


<210>  8
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PSA cleavage site

<400>  8

His Ser Ser Lys Leu Gln Ser Ala 
1               5               


<210>  9
<211>  1410
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Proaerolysin with a PSA sequence substituted for the furin site.

<400>  9
gcagagcccg tctatccaga ccagcttcgc ttgttttcat tgggccaagg ggtctgtggc       60

gacaagtatc gccccgtcaa tcgagaagaa gcccaaagcg ttaaaagcaa tattgtcggc      120

atgatggggc aatggcaaat aagcgggctg gccaacggct gggtcattat ggggccgggt      180

tataacggtg aaataaaacc agggacagcg tccaatacct ggtgttatcc gaccaatcct      240

gttaccggtg aaataccgac actgtctgcc ctggatattc cagatggtga cgaagtcgat      300

gtgcagtggc gactggtaca tgacagtgcg aatttcatca aaccaaccag ctatctggcc      360

cattacctcg gttatgcctg ggtgggcggc aatcacagcc aatatgtcgg cgaagacatg      420

gatgtgaccc gtgatggcga cggctgggtg atccgtggca acaatgacgg cggctgtgac      480

ggctatcgct gtggtgacaa gacggccatc aaggtcagca acttcgccta taacctggat      540

cccgacagct tcaagcatgg cgatgtcacc cagtccgacc gccagctggt caagactgtg      600

gtgggctggg cggtcaacga cagcgacacc ccccaatccg gctatgacgt caccctgcgc      660

tacgacacag ccaccaactg gtccaagacc aacacctatg gcctgagcga gaaggtgacc      720

accaagaaca agttcaagtg gccactggtg ggggaaaccc aactctccat cgagattgct      780

gccaatcagt cctgggcgtc ccagaacggg ggctcgacca ccacctccct gtctcagtcc      840

gtgcgaccga ctgtgccggc ccgctccaag atcccggtga agatagagct ctacaaggcc      900

gacatctcct atccctatga gttcaaggcc gatgtcagct atgacctgac cctgagcggc      960

ttcctgcgct ggggcggcaa cgcctggtat acccacccgg acaaccgtcc gaactggaac     1020

cacaccttcg tcataggtcc gtacaaggac aaggcgagca gcattcggta ccagtgggac     1080

aagcgttaca tcccgggtga agtgaagtgg tgggactgga actggaccat acagcagaac     1140

ggtctgtcta ccatgcagaa caacctggcc agagtgctgc gcccggtgcg ggcggggatc     1200

accggtgatt tcagtgccga gagccagttt gccggcaaca tagagatcgg tgctcccgtg     1260

ccgctcgcgg ctgactccca gttctatagc agcaatagtg tggacggcgc tggtcaaggc     1320

ctgaggctgg agatcccgct cgatgcgcaa gagctctccg ggcttggctt caacaacgtc     1380

agcctcagcg tgacccctgc tgccaatcaa                                      1410


<210>  10
<211>  470
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Proaerolysin with a PSA sequence substituted for the furin site.

<400>  10

Ala Glu Pro Val Tyr Pro Asp Gln Leu Arg Leu Phe Ser Leu Gly Gln 
1               5                   10                  15      


Gly Val Cys Gly Asp Lys Tyr Arg Pro Val Asn Arg Glu Glu Ala Gln 
            20                  25                  30          


Ser Val Lys Ser Asn Ile Val Gly Met Met Gly Gln Trp Gln Ile Ser 
        35                  40                  45              


Gly Leu Ala Asn Gly Trp Val Ile Met Gly Pro Gly Tyr Asn Gly Glu 
    50                  55                  60                  


Ile Lys Pro Gly Thr Ala Ser Asn Thr Trp Cys Tyr Pro Thr Asn Pro 
65                  70                  75                  80  


Val Thr Gly Glu Ile Pro Thr Leu Ser Ala Leu Asp Ile Pro Asp Gly 
                85                  90                  95      


Asp Glu Val Asp Val Gln Trp Arg Leu Val His Asp Ser Ala Asn Phe 
            100                 105                 110         


Ile Lys Pro Thr Ser Tyr Leu Ala His Tyr Leu Gly Tyr Ala Trp Val 
        115                 120                 125             


Gly Gly Asn His Ser Gln Tyr Val Gly Glu Asp Met Asp Val Thr Arg 
    130                 135                 140                 


Asp Gly Asp Gly Trp Val Ile Arg Gly Asn Asn Asp Gly Gly Cys Asp 
145                 150                 155                 160 


Gly Tyr Arg Cys Gly Asp Lys Thr Ala Ile Lys Val Ser Asn Phe Ala 
                165                 170                 175     


Tyr Asn Leu Asp Pro Asp Ser Phe Lys His Gly Asp Val Thr Gln Ser 
            180                 185                 190         


Asp Arg Gln Leu Val Lys Thr Val Val Gly Trp Ala Val Asn Asp Ser 
        195                 200                 205             


Asp Thr Pro Gln Ser Gly Tyr Asp Val Thr Leu Arg Tyr Asp Thr Ala 
    210                 215                 220                 


Thr Asn Trp Ser Lys Thr Asn Thr Tyr Gly Leu Ser Glu Lys Val Thr 
225                 230                 235                 240 


Thr Lys Asn Lys Phe Lys Trp Pro Leu Val Gly Glu Thr Gln Leu Ser 
                245                 250                 255     


Ile Glu Ile Ala Ala Asn Gln Ser Trp Ala Ser Gln Asn Gly Gly Ser 
            260                 265                 270         


Thr Thr Thr Ser Leu Ser Gln Ser Val Arg Pro Thr Val Pro Ala Arg 
        275                 280                 285             


Ser Lys Ile Pro Val Lys Ile Glu Leu Tyr Lys Ala Asp Ile Ser Tyr 
    290                 295                 300                 


Pro Tyr Glu Phe Lys Ala Asp Val Ser Tyr Asp Leu Thr Leu Ser Gly 
305                 310                 315                 320 


Phe Leu Arg Trp Gly Gly Asn Ala Trp Tyr Thr His Pro Asp Asn Arg 
                325                 330                 335     


Pro Asn Trp Asn His Thr Phe Val Ile Gly Pro Tyr Lys Asp Lys Ala 
            340                 345                 350         


Ser Ser Ile Arg Tyr Gln Trp Asp Lys Arg Tyr Ile Pro Gly Glu Val 
        355                 360                 365             


Lys Trp Trp Asp Trp Asn Trp Thr Ile Gln Gln Asn Gly Leu Ser Thr 
    370                 375                 380                 


Met Gln Asn Asn Leu Ala Arg Val Leu Arg Pro Val Arg Ala Gly Ile 
385                 390                 395                 400 


Thr Gly Asp Phe Ser Ala Glu Ser Gln Phe Ala Gly Asn Ile Glu Ile 
                405                 410                 415     


Gly Ala Pro Val Pro Leu Ala Ala Asp Ser Gln Phe Tyr Ser Ser Asn 
            420                 425                 430         


Ser Val Asp Gly Ala Gly Gln Gly Leu Arg Leu Glu Ile Pro Leu Asp 
        435                 440                 445             


Ala Gln Glu Leu Ser Gly Leu Gly Phe Asn Asn Val Ser Leu Ser Val 
    450                 455                 460                 


Thr Pro Ala Ala Asn Gln 
465                 470 


<210>  11
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PSA cleavage site

<400>  11

Gln Phe Tyr Ser Ser Asn 
1               5       


<210>  12
<211>  1410
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Proaerolysin with a PSA sequence substituted for the furin site.

<400>  12
gcagagcccg tctatccaga ccagcttcgc ttgttttcat tgggccaagg ggtctgtggc       60

gacaagtatc gccccgtcaa tcgagaagaa gcccaaagcg ttaaaagcaa tattgtcggc      120

atgatggggc aatggcaaat aagcgggctg gccaacggct gggtcattat ggggccgggt      180

tataacggtg aaataaaacc agggacagcg tccaatacct ggtgttatcc gaccaatcct      240

gttaccggtg aaataccgac actgtctgcc ctggatattc cagatggtga cgaagtcgat      300

gtgcagtggc gactggtaca tgacagtgcg aatttcatca aaccaaccag ctatctggcc      360

cattacctcg gttatgcctg ggtgggcggc aatcacagcc aatatgtcgg cgaagacatg      420

gatgtgaccc gtgatggcga cggctgggtg atccgtggca acaatgacgg cggctgtgac      480

ggctatcgct gtggtgacaa gacggccatc aaggtcagca acttcgccta taacctggat      540

cccgacagct tcaagcatgg cgatgtcacc cagtccgacc gccagctggt caagactgtg      600

gtgggctggg cggtcaacga cagcgacacc ccccaatccg gctatgacgt caccctgcgc      660

tacgacacag ccaccaactg gtccaagacc aacacctatg gcctgagcga gaaggtgacc      720

accaagaaca agttcaagtg gccactggtg ggggaaaccc aactctccat cgagattgct      780

gccaatcagt cctgggcgtc ccagaacggg ggctcgacca ccacctccct gtctcagtcc      840

gtgcgaccga ctgtgccggc ccgctccaag atcccggtga agatagagct ctacaaggcc      900

gacatctcct atccctatga gttcaaggcc gatgtcagct atgacctgac cctgagcggc      960

ttcctgcgct ggggcggcaa cgcctggtat acccacccgg acaaccgtcc gaactggaac     1020

cacaccttcg tcataggtcc gtacaaggac aaggcgagca gcattcggta ccagtgggac     1080

aagcgttaca tcccgggtga agtgaagtgg tgggactgga actggaccat acagcagaac     1140

ggtctgtcta ccatgcagaa caacctggcc agagtgctgc gcccggtgcg ggcggggatc     1200

accggtgatt tcagtgccga gagccagttt gccggcaaca tagagatcgg tgctcccgtg     1260

ccgctcgcgg ctgacggtat aagtagtttc cagagtagtg tggacggcgc tggtcaaggc     1320

ctgaggctgg agatcccgct cgatgcgcaa gagctctccg ggcttggctt caacaacgtc     1380

agcctcagcg tgacccctgc tgccaatcaa                                      1410


<210>  13
<211>  470
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Proaerolysin with a PSA sequence substituted for the furin site.

<400>  13

Ala Glu Pro Val Tyr Pro Asp Gln Leu Arg Leu Phe Ser Leu Gly Gln 
1               5                   10                  15      


Gly Val Cys Gly Asp Lys Tyr Arg Pro Val Asn Arg Glu Glu Ala Gln 
            20                  25                  30          


Ser Val Lys Ser Asn Ile Val Gly Met Met Gly Gln Trp Gln Ile Ser 
        35                  40                  45              


Gly Leu Ala Asn Gly Trp Val Ile Met Gly Pro Gly Tyr Asn Gly Glu 
    50                  55                  60                  


Ile Lys Pro Gly Thr Ala Ser Asn Thr Trp Cys Tyr Pro Thr Asn Pro 
65                  70                  75                  80  


Val Thr Gly Glu Ile Pro Thr Leu Ser Ala Leu Asp Ile Pro Asp Gly 
                85                  90                  95      


Asp Glu Val Asp Val Gln Trp Arg Leu Val His Asp Ser Ala Asn Phe 
            100                 105                 110         


Ile Lys Pro Thr Ser Tyr Leu Ala His Tyr Leu Gly Tyr Ala Trp Val 
        115                 120                 125             


Gly Gly Asn His Ser Gln Tyr Val Gly Glu Asp Met Asp Val Thr Arg 
    130                 135                 140                 


Asp Gly Asp Gly Trp Val Ile Arg Gly Asn Asn Asp Gly Gly Cys Asp 
145                 150                 155                 160 


Gly Tyr Arg Cys Gly Asp Lys Thr Ala Ile Lys Val Ser Asn Phe Ala 
                165                 170                 175     


Tyr Asn Leu Asp Pro Asp Ser Phe Lys His Gly Asp Val Thr Gln Ser 
            180                 185                 190         


Asp Arg Gln Leu Val Lys Thr Val Val Gly Trp Ala Val Asn Asp Ser 
        195                 200                 205             


Asp Thr Pro Gln Ser Gly Tyr Asp Val Thr Leu Arg Tyr Asp Thr Ala 
    210                 215                 220                 


Thr Asn Trp Ser Lys Thr Asn Thr Tyr Gly Leu Ser Glu Lys Val Thr 
225                 230                 235                 240 


Thr Lys Asn Lys Phe Lys Trp Pro Leu Val Gly Glu Thr Gln Leu Ser 
                245                 250                 255     


Ile Glu Ile Ala Ala Asn Gln Ser Trp Ala Ser Gln Asn Gly Gly Ser 
            260                 265                 270         


Thr Thr Thr Ser Leu Ser Gln Ser Val Arg Pro Thr Val Pro Ala Arg 
        275                 280                 285             


Ser Lys Ile Pro Val Lys Ile Glu Leu Tyr Lys Ala Asp Ile Ser Tyr 
    290                 295                 300                 


Pro Tyr Glu Phe Lys Ala Asp Val Ser Tyr Asp Leu Thr Leu Ser Gly 
305                 310                 315                 320 


Phe Leu Arg Trp Gly Gly Asn Ala Trp Tyr Thr His Pro Asp Asn Arg 
                325                 330                 335     


Pro Asn Trp Asn His Thr Phe Val Ile Gly Pro Tyr Lys Asp Lys Ala 
            340                 345                 350         


Ser Ser Ile Arg Tyr Gln Trp Asp Lys Arg Tyr Ile Pro Gly Glu Val 
        355                 360                 365             


Lys Trp Trp Asp Trp Asn Trp Thr Ile Gln Gln Asn Gly Leu Ser Thr 
    370                 375                 380                 


Met Gln Asn Asn Leu Ala Arg Val Leu Arg Pro Val Arg Ala Gly Ile 
385                 390                 395                 400 


Thr Gly Asp Phe Ser Ala Glu Ser Gln Phe Ala Gly Asn Ile Glu Ile 
                405                 410                 415     


Gly Ala Pro Val Pro Leu Ala Ala Asp Gly Ile Ser Ser Phe Gln Ser 
            420                 425                 430         


Ser Val Asp Gly Ala Gly Gln Gly Leu Arg Leu Glu Ile Pro Leu Asp 
        435                 440                 445             


Ala Gln Glu Leu Ser Gly Leu Gly Phe Asn Asn Val Ser Leu Ser Val 
    450                 455                 460                 


Thr Pro Ala Ala Asn Gln 
465                 470 


<210>  14
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PSA cleavage site

<400>  14

Gly Ile Ser Ser Phe Gln Ser 
1               5           


<210>  15
<211>  7
<212>  PRT
<213>  Homo sapiens

<400>  15

Lys Gly Ile Ser Ser Gln Tyr 
1               5           


<210>  16
<211>  7
<212>  PRT
<213>  Homo sapiens

<400>  16

Ser Arg Lys Ser Gln Gln Tyr 
1               5           


<210>  17
<211>  7
<212>  PRT
<213>  Homo sapiens

<400>  17

Ala Thr Lys Ser Lys Gln His 
1               5           


<210>  18
<211>  7
<212>  PRT
<213>  Homo sapiens

<400>  18

Lys Gly Leu Ser Ser Gln Cys 
1               5           


<210>  19
<211>  7
<212>  PRT
<213>  Homo sapiens

<400>  19

Leu Gly Gly Ser Ser Gln Leu 
1               5           


<210>  20
<211>  7
<212>  PRT
<213>  Homo sapiens

<400>  20

Glu His Ser Ser Lys Leu Gln 
1               5           


<210>  21
<211>  4
<212>  PRT
<213>  Homo sapiens

<400>  21

Ser Lys Leu Gln 
1               


<210>  22
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  LHRH variant sequence

<400>  22

Gln His Trp Ser Tyr Gly Leu Arg Pro Gly 
1               5                   10  


<210>  23
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LHRH variant sequence

<400>  23

Glu His Trp Ser Tyr Lys Leu Arg Pro Gly 
1               5                   10  


<210>  24
<211>  397
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant proaerolysin peptide

<400>  24

Glu His Trp Ser Tyr Lys Leu Arg Pro Gly Glu Ile Pro Thr Leu Ser 
1               5                   10                  15      


Ala Leu Asp Ile Pro Asp Gly Asp Glu Val Asp Val Gln Trp Arg Leu 
            20                  25                  30          


Val His Asp Ser Ala Asn Phe Ile Lys Pro Thr Ser Tyr Leu Ala His 
        35                  40                  45              


Tyr Leu Gly Tyr Ala Trp Val Gly Gly Asn His Ser Gln Tyr Val Gly 
    50                  55                  60                  


Glu Asp Met Asp Val Thr Arg Asp Gly Asp Gly Trp Val Ile Arg Gly 
65                  70                  75                  80  


Asn Asn Asp Gly Gly Cys Asp Gly Tyr Arg Cys Gly Asp Lys Thr Ala 
                85                  90                  95      


Ile Lys Val Ser Asn Phe Ala Tyr Asn Leu Asp Pro Asp Ser Phe Lys 
            100                 105                 110         


His Gly Asp Val Thr Gln Ser Asp Arg Gln Leu Val Lys Thr Val Val 
        115                 120                 125             


Gly Trp Ala Val Asn Asp Ser Asp Thr Pro Gln Ser Gly Tyr Asp Val 
    130                 135                 140                 


Thr Leu Arg Tyr Asp Thr Ala Thr Asn Trp Ser Lys Thr Asn Thr Tyr 
145                 150                 155                 160 


Gly Leu Ser Glu Lys Val Thr Thr Lys Asn Lys Phe Lys Trp Pro Leu 
                165                 170                 175     


Val Gly Glu Thr Gln Leu Ser Ile Glu Ile Ala Ala Asn Gln Ser Trp 
            180                 185                 190         


Ala Ser Gln Asn Gly Gly Ser Thr Thr Thr Ser Leu Ser Gln Ser Val 
        195                 200                 205             


Arg Pro Thr Val Pro Ala Arg Ser Lys Ile Pro Val Lys Ile Glu Leu 
    210                 215                 220                 


Tyr Lys Ala Asp Ile Ser Tyr Pro Tyr Glu Phe Lys Ala Asp Val Ser 
225                 230                 235                 240 


Tyr Asp Leu Thr Leu Ser Gly Phe Leu Arg Trp Gly Gly Asn Ala Trp 
                245                 250                 255     


Tyr Thr His Pro Asp Asn Arg Pro Asn Trp Asn His Thr Phe Val Ile 
            260                 265                 270         


Gly Pro Tyr Lys Asp Lys Ala Ser Ser Ile Arg Tyr Gln Trp Asp Lys 
        275                 280                 285             


Arg Tyr Ile Pro Gly Glu Val Lys Trp Trp Asp Trp Asn Trp Thr Ile 
    290                 295                 300                 


Gln Gln Asn Gly Leu Ser Thr Met Gln Asn Asn Leu Ala Arg Val Leu 
305                 310                 315                 320 


Arg Pro Val Arg Ala Gly Ile Thr Gly Asp Phe Ser Ala Glu Ser Gln 
                325                 330                 335     


Phe Ala Gly Asn Ile Glu Ile Gly Ala Pro Val Pro Leu Ala Ala Asp 
            340                 345                 350         


Ser His Ser Ser Lys Leu Gln Ser Val Asp Gly Ala Gly Gln Gly Leu 
        355                 360                 365             


Arg Leu Glu Ile Pro Leu Asp Ala Gln Glu Leu Ser Gly Leu Gly Phe 
    370                 375                 380                 


Asn Asn Val Ser Leu Ser Val Thr Pro Ala Ala Asn Gln 
385                 390                 395         


<210>  25
<211>  397
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant proaerolysin peptide

<400>  25

Glu His Trp Ser Tyr Lys Leu Arg Pro Gly Glu Ile Pro Thr Leu Ser 
1               5                   10                  15      


Ala Leu Asp Ile Pro Asp Gly Asp Glu Val Asp Val Gln Trp Arg Leu 
            20                  25                  30          


Val His Asp Ser Ala Asn Phe Ile Lys Pro Thr Ser Tyr Leu Ala His 
        35                  40                  45              


Tyr Leu Gly Tyr Ala Trp Val Gly Gly Asn His Ser Gln Tyr Val Gly 
    50                  55                  60                  


Glu Asp Met Asp Val Thr Arg Asp Gly Asp Gly Trp Val Ile Arg Gly 
65                  70                  75                  80  


Asn Asn Asp Gly Gly Cys Asp Gly Tyr Arg Cys Gly Asp Lys Thr Ala 
                85                  90                  95      


Ile Lys Val Ser Asn Phe Ala Tyr Asn Leu Asp Pro Asp Ser Phe Lys 
            100                 105                 110         


His Gly Asp Val Thr Gln Ser Asp Arg Gln Leu Val Lys Thr Val Val 
        115                 120                 125             


Gly Trp Ala Val Asn Asp Ser Asp Thr Pro Gln Ser Gly Tyr Asp Val 
    130                 135                 140                 


Thr Leu Arg Tyr Asp Thr Ala Thr Asn Trp Ser Lys Thr Asn Thr Tyr 
145                 150                 155                 160 


Gly Leu Ser Glu Lys Val Thr Thr Lys Asn Lys Phe Lys Trp Pro Leu 
                165                 170                 175     


Val Gly Glu Thr Gln Leu Ser Ile Glu Ile Ala Ala Asn Gln Ser Trp 
            180                 185                 190         


Ala Ser Gln Asn Gly Gly Ser Thr Thr Thr Ser Leu Ser Gln Ser Val 
        195                 200                 205             


Arg Pro Thr Val Pro Ala Arg Ser Lys Ile Pro Val Lys Ile Glu Leu 
    210                 215                 220                 


Tyr Lys Ala Asp Ile Ser Tyr Pro Tyr Glu Phe Lys Ala Asp Val Ser 
225                 230                 235                 240 


Tyr Asp Leu Thr Leu Ser Gly Phe Leu Arg Trp Gly Gly Asn Ala Trp 
                245                 250                 255     


Tyr Thr His Pro Asp Asn Arg Pro Asn Trp Asn His Thr Phe Val Ile 
            260                 265                 270         


Gly Pro Tyr Lys Asp Lys Ala Ser Ser Ile Arg Tyr Gln Trp Asp Lys 
        275                 280                 285             


Arg Tyr Ile Pro Gly Glu Val Lys Trp Trp Asp Trp Asn Trp Thr Ile 
    290                 295                 300                 


Gln Gln Asn Gly Leu Ser Thr Met Gln Asn Asn Leu Ala Arg Val Leu 
305                 310                 315                 320 


Arg Pro Val Arg Ala Gly Ile Thr Gly Asp Phe Ser Ala Glu Ser Gln 
                325                 330                 335     


Phe Ala Gly Asn Ile Glu Ile Gly Ala Pro Val Pro Leu Ala Ala Asp 
            340                 345                 350         


Ser Lys Val Arg Arg Ala Arg Ser Val Asp Gly Ala Gly Gln Gly Leu 
        355                 360                 365             


Arg Leu Glu Ile Pro Leu Asp Ala Gln Glu Leu Ser Gly Leu Gly Phe 
    370                 375                 380                 


Asn Asn Val Ser Leu Ser Val Thr Pro Ala Ala Asn Gln 
385                 390                 395         


<210>  26
<211>  476
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Proaerolysin with PSA sequence substituted for the furin site and
       an N-terminal His tag.

<400>  26

His His His His His His Ala Glu Pro Val Tyr Pro Asp Gln Leu Arg 
1               5                   10                  15      


Leu Phe Ser Leu Gly Gln Gly Val Cys Gly Asp Lys Tyr Arg Pro Val 
            20                  25                  30          


Asn Arg Glu Glu Ala Gln Ser Val Lys Ser Asn Ile Val Gly Met Met 
        35                  40                  45              


Gly Gln Trp Gln Ile Ser Gly Leu Ala Asn Gly Trp Val Ile Met Gly 
    50                  55                  60                  


Pro Gly Tyr Asn Gly Glu Ile Lys Pro Gly Thr Ala Ser Asn Thr Trp 
65                  70                  75                  80  


Cys Tyr Pro Thr Asn Pro Val Thr Gly Glu Ile Pro Thr Leu Ser Ala 
                85                  90                  95      


Leu Asp Ile Pro Asp Gly Asp Glu Val Asp Val Gln Trp Arg Leu Val 
            100                 105                 110         


His Asp Ser Ala Asn Phe Ile Lys Pro Thr Ser Tyr Leu Ala His Tyr 
        115                 120                 125             


Leu Gly Tyr Ala Trp Val Gly Gly Asn His Ser Gln Tyr Val Gly Glu 
    130                 135                 140                 


Asp Met Asp Val Thr Arg Asp Gly Asp Gly Trp Val Ile Arg Gly Asn 
145                 150                 155                 160 


Asn Asp Gly Gly Cys Asp Gly Tyr Arg Cys Gly Asp Lys Thr Ala Ile 
                165                 170                 175     


Lys Val Ser Asn Phe Ala Tyr Asn Leu Asp Pro Asp Ser Phe Lys His 
            180                 185                 190         


Gly Asp Val Thr Gln Ser Asp Arg Gln Leu Val Lys Thr Val Val Gly 
        195                 200                 205             


Trp Ala Val Asn Asp Ser Asp Thr Pro Gln Ser Gly Tyr Asp Val Thr 
    210                 215                 220                 


Leu Arg Tyr Asp Thr Ala Thr Asn Trp Ser Lys Thr Asn Thr Tyr Gly 
225                 230                 235                 240 


Leu Ser Glu Lys Val Thr Thr Lys Asn Lys Phe Lys Trp Pro Leu Val 
                245                 250                 255     


Gly Glu Thr Gln Leu Ser Ile Glu Ile Ala Ala Asn Gln Ser Trp Ala 
            260                 265                 270         


Ser Gln Asn Gly Gly Ser Thr Thr Thr Ser Leu Ser Gln Ser Val Arg 
        275                 280                 285             


Pro Thr Val Pro Ala Arg Ser Lys Ile Pro Val Lys Ile Glu Leu Tyr 
    290                 295                 300                 


Lys Ala Asp Ile Ser Tyr Pro Tyr Glu Phe Lys Ala Asp Val Ser Tyr 
305                 310                 315                 320 


Asp Leu Thr Leu Ser Gly Phe Leu Arg Trp Gly Gly Asn Ala Trp Tyr 
                325                 330                 335     


Thr His Pro Asp Asn Arg Pro Asn Trp Asn His Thr Phe Val Ile Gly 
            340                 345                 350         


Pro Tyr Lys Asp Lys Ala Ser Ser Ile Arg Tyr Gln Trp Asp Lys Arg 
        355                 360                 365             


Tyr Ile Pro Gly Glu Val Lys Trp Trp Asp Trp Asn Trp Thr Ile Gln 
    370                 375                 380                 


Gln Asn Gly Leu Ser Thr Met Gln Asn Asn Leu Ala Arg Val Leu Arg 
385                 390                 395                 400 


Pro Val Arg Ala Gly Ile Thr Gly Asp Phe Ser Ala Glu Ser Gln Phe 
                405                 410                 415     


Ala Gly Asn Ile Glu Ile Gly Ala Pro Val Pro Leu Ala Ala Asp Ser 
            420                 425                 430         


His Ser Ser Lys Leu Gln Ser Val Asp Gly Ala Gly Gln Gly Leu Arg 
        435                 440                 445             


Leu Glu Ile Pro Leu Asp Ala Gln Glu Leu Ser Gly Leu Gly Phe Asn 
    450                 455                 460                 


Asn Val Ser Leu Ser Val Thr Pro Ala Ala Asn Gln 
465                 470                 475     


<210>  27
<211>  585
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(585)
<223>  Human Albumin mature sequence

<400>  27

Asp Ala His Lys Ser Glu Val Ala His Arg Phe Lys Asp Leu Gly Glu 
1               5                   10                  15      


Glu Asn Phe Lys Ala Leu Val Leu Ile Ala Phe Ala Gln Tyr Leu Gln 
            20                  25                  30          


Gln Cys Pro Phe Glu Asp His Val Lys Leu Val Asn Glu Val Thr Glu 
        35                  40                  45              


Phe Ala Lys Thr Cys Val Ala Asp Glu Ser Ala Glu Asn Cys Asp Lys 
    50                  55                  60                  


Ser Leu His Thr Leu Phe Gly Asp Lys Leu Cys Thr Val Ala Thr Leu 
65                  70                  75                  80  


Arg Glu Thr Tyr Gly Glu Met Ala Asp Cys Cys Ala Lys Gln Glu Pro 
                85                  90                  95      


Glu Arg Asn Glu Cys Phe Leu Gln His Lys Asp Asp Asn Pro Asn Leu 
            100                 105                 110         


Pro Arg Leu Val Arg Pro Glu Val Asp Val Met Cys Thr Ala Phe His 
        115                 120                 125             


Asp Asn Glu Glu Thr Phe Leu Lys Lys Tyr Leu Tyr Glu Ile Ala Arg 
    130                 135                 140                 


Arg His Pro Tyr Phe Tyr Ala Pro Glu Leu Leu Phe Phe Ala Lys Arg 
145                 150                 155                 160 


Tyr Lys Ala Ala Phe Thr Glu Cys Cys Gln Ala Ala Asp Lys Ala Ala 
                165                 170                 175     


Cys Leu Leu Pro Lys Leu Asp Glu Leu Arg Asp Glu Gly Lys Ala Ser 
            180                 185                 190         


Ser Ala Lys Gln Arg Leu Lys Cys Ala Ser Leu Gln Lys Phe Gly Glu 
        195                 200                 205             


Arg Ala Phe Lys Ala Trp Ala Val Ala Arg Leu Ser Gln Arg Phe Pro 
    210                 215                 220                 


Lys Ala Glu Phe Ala Glu Val Ser Lys Leu Val Thr Asp Leu Thr Lys 
225                 230                 235                 240 


Val His Thr Glu Cys Cys His Gly Asp Leu Leu Glu Cys Ala Asp Asp 
                245                 250                 255     


Arg Ala Asp Leu Ala Lys Tyr Ile Cys Glu Asn Gln Asp Ser Ile Ser 
            260                 265                 270         


Ser Lys Leu Lys Glu Cys Cys Glu Lys Pro Leu Leu Glu Lys Ser His 
        275                 280                 285             


Cys Ile Ala Glu Val Glu Asn Asp Glu Met Arg Ala Asp Leu Pro Ser 
    290                 295                 300                 


Leu Ala Ala Asp Phe Val Glu Ser Lys Asp Val Cys Lys Asn Tyr Ala 
305                 310                 315                 320 


Glu Ala Lys Asp Val Phe Leu Gly Met Phe Leu Tyr Glu Tyr Ala Arg 
                325                 330                 335     


Arg His Pro Asp Tyr Ser Val Val Leu Leu Leu Arg Leu Ala Lys Thr 
            340                 345                 350         


Tyr Glu Thr Thr Leu Glu Lys Cys Cys Ala Ala Ala Asp Pro His Glu 
        355                 360                 365             


Cys Tyr Ala Lys Val Phe Asp Glu Phe Lys Pro Leu Val Glu Glu Pro 
    370                 375                 380                 


Gln Asn Leu Ile Lys Gln Asn Cys Glu Leu Phe Glu Gln Leu Gly Glu 
385                 390                 395                 400 


Tyr Lys Phe Gln Asn Ala Leu Leu Val Arg Tyr Thr Lys Lys Val Pro 
                405                 410                 415     


Gln Val Ser Thr Pro Thr Leu Val Glu Val Ser Arg Asn Leu Gly Lys 
            420                 425                 430         


Val Gly Ser Lys Cys Cys Lys His Pro Glu Ala Lys Arg Met Pro Cys 
        435                 440                 445             


Ala Glu Asp Tyr Leu Ser Val Val Leu Asn Gln Leu Cys Val Leu His 
    450                 455                 460                 


Glu Lys Thr Pro Val Ser Asp Arg Val Thr Lys Cys Cys Thr Glu Ser 
465                 470                 475                 480 


Leu Val Asn Arg Arg Pro Cys Phe Ser Ala Leu Glu Val Asp Glu Thr 
                485                 490                 495     


Tyr Val Pro Lys Glu Phe Asn Ala Glu Thr Phe Thr Phe His Ala Asp 
            500                 505                 510         


Ile Cys Thr Leu Ser Glu Lys Glu Arg Gln Ile Lys Lys Gln Thr Ala 
        515                 520                 525             


Leu Val Glu Leu Val Lys His Lys Pro Lys Ala Thr Lys Glu Gln Leu 
    530                 535                 540                 


Lys Ala Val Met Asp Asp Phe Ala Ala Phe Val Glu Lys Cys Cys Lys 
545                 550                 555                 560 


Ala Asp Asp Lys Glu Thr Cys Phe Ala Glu Glu Gly Lys Lys Leu Val 
                565                 570                 575     


Ala Ala Ser Gln Ala Ala Leu Gly Leu 
            580                 585 


<210>  28
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HK2-specific cleavage site

<400>  28

Gln Lys Arg Arg 
1               


<210>  29
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HK2-specific cleavage site

<400>  29

Lys Ser Arg Arg 
1               


<210>  30
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HK2-specific cleavage site

<400>  30

Ala Lys Arg Arg 
1               


<210>  31
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HK2-specific cleavage site

<400>  31

Lys Lys Arg Arg 
1               


<210>  32
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HK2-specific cleavage site

<400>  32

His Lys Arg Arg 
1               


<210>  33
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HK2-specific cleavage site

<400>  33

Lys Ala Phe Arg 
1               


<210>  34
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HK2-specific cleavage site

<400>  34

Lys Ala Gln Arg 
1               


<210>  35
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HK2-specific cleavage site

<400>  35

Lys Ala Lys Arg 
1               


<210>  36
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HK2-specific cleavage site

<400>  36

Lys Ala Arg Lys 
1               


<210>  37
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HK2-specific cleavage site

<400>  37

Lys Ala His Arg 
1               


<210>  38
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HK2-specific cleavage site

<400>  38

His Ala Gln Lys Arg Arg 
1               5       


<210>  39
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HK2-specific cleavage site

<400>  39

Gly Gly Lys Ser Arg Arg 
1               5       


<210>  40
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HK2-specific cleavage site

<400>  40

His Glu Gln Lys Arg Arg 
1               5       


<210>  41
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HK2-specific cleavage site

<400>  41

His Glu Ala Lys Arg Arg 
1               5       


<210>  42
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HK2-specific cleavage site

<400>  42

Gly Gly Gln Lys Arg Arg 
1               5       


<210>  43
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HK2-specific cleavage site

<400>  43

His Glu Gln Lys Arg Arg 
1               5       


<210>  44
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HK2-specific cleavage site

<400>  44

Gly Gly Ala Lys Arg Arg 
1               5       


<210>  45
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HK2-specific cleavage site

<400>  45

His Glu Gln Lys Arg Arg 
1               5       


<210>  46
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HK2-specific cleavage site

<400>  46

Gly Gly Lys Lys Arg Arg 
1               5       


<210>  47
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HK2-specific cleavage site

<400>  47

Gly Gly His Lys Arg Arg 
1               5       


<210>  48
<211>  1061
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HSA fused to PRC302 with one PSA cleavage site linker

<400>  48

Asp Ala His Lys Ser Glu Val Ala His Arg Phe Lys Asp Leu Gly Glu 
1               5                   10                  15      


Glu Asn Phe Lys Ala Leu Val Leu Ile Ala Phe Ala Gln Tyr Leu Gln 
            20                  25                  30          


Gln Cys Pro Phe Glu Asp His Val Lys Leu Val Asn Glu Val Thr Glu 
        35                  40                  45              


Phe Ala Lys Thr Cys Val Ala Asp Glu Ser Ala Glu Asn Cys Asp Lys 
    50                  55                  60                  


Ser Leu His Thr Leu Phe Gly Asp Lys Leu Cys Thr Val Ala Thr Leu 
65                  70                  75                  80  


Arg Glu Thr Tyr Gly Glu Met Ala Asp Cys Cys Ala Lys Gln Glu Pro 
                85                  90                  95      


Glu Arg Asn Glu Cys Phe Leu Gln His Lys Asp Asp Asn Pro Asn Leu 
            100                 105                 110         


Pro Arg Leu Val Arg Pro Glu Val Asp Val Met Cys Thr Ala Phe His 
        115                 120                 125             


Asp Asn Glu Glu Thr Phe Leu Lys Lys Tyr Leu Tyr Glu Ile Ala Arg 
    130                 135                 140                 


Arg His Pro Tyr Phe Tyr Ala Pro Glu Leu Leu Phe Phe Ala Lys Arg 
145                 150                 155                 160 


Tyr Lys Ala Ala Phe Thr Glu Cys Cys Gln Ala Ala Asp Lys Ala Ala 
                165                 170                 175     


Cys Leu Leu Pro Lys Leu Asp Glu Leu Arg Asp Glu Gly Lys Ala Ser 
            180                 185                 190         


Ser Ala Lys Gln Arg Leu Lys Cys Ala Ser Leu Gln Lys Phe Gly Glu 
        195                 200                 205             


Arg Ala Phe Lys Ala Trp Ala Val Ala Arg Leu Ser Gln Arg Phe Pro 
    210                 215                 220                 


Lys Ala Glu Phe Ala Glu Val Ser Lys Leu Val Thr Asp Leu Thr Lys 
225                 230                 235                 240 


Val His Thr Glu Cys Cys His Gly Asp Leu Leu Glu Cys Ala Asp Asp 
                245                 250                 255     


Arg Ala Asp Leu Ala Lys Tyr Ile Cys Glu Asn Gln Asp Ser Ile Ser 
            260                 265                 270         


Ser Lys Leu Lys Glu Cys Cys Glu Lys Pro Leu Leu Glu Lys Ser His 
        275                 280                 285             


Cys Ile Ala Glu Val Glu Asn Asp Glu Met Arg Ala Asp Leu Pro Ser 
    290                 295                 300                 


Leu Ala Ala Asp Phe Val Glu Ser Lys Asp Val Cys Lys Asn Tyr Ala 
305                 310                 315                 320 


Glu Ala Lys Asp Val Phe Leu Gly Met Phe Leu Tyr Glu Tyr Ala Arg 
                325                 330                 335     


Arg His Pro Asp Tyr Ser Val Val Leu Leu Leu Arg Leu Ala Lys Thr 
            340                 345                 350         


Tyr Glu Thr Thr Leu Glu Lys Cys Cys Ala Ala Ala Asp Pro His Glu 
        355                 360                 365             


Cys Tyr Ala Lys Val Phe Asp Glu Phe Lys Pro Leu Val Glu Glu Pro 
    370                 375                 380                 


Gln Asn Leu Ile Lys Gln Asn Cys Glu Leu Phe Glu Gln Leu Gly Glu 
385                 390                 395                 400 


Tyr Lys Phe Gln Asn Ala Leu Leu Val Arg Tyr Thr Lys Lys Val Pro 
                405                 410                 415     


Gln Val Ser Thr Pro Thr Leu Val Glu Val Ser Arg Asn Leu Gly Lys 
            420                 425                 430         


Val Gly Ser Lys Cys Cys Lys His Pro Glu Ala Lys Arg Met Pro Cys 
        435                 440                 445             


Ala Glu Asp Tyr Leu Ser Val Val Leu Asn Gln Leu Cys Val Leu His 
    450                 455                 460                 


Glu Lys Thr Pro Val Ser Asp Arg Val Thr Lys Cys Cys Thr Glu Ser 
465                 470                 475                 480 


Leu Val Asn Arg Arg Pro Cys Phe Ser Ala Leu Glu Val Asp Glu Thr 
                485                 490                 495     


Tyr Val Pro Lys Glu Phe Asn Ala Glu Thr Phe Thr Phe His Ala Asp 
            500                 505                 510         


Ile Cys Thr Leu Ser Glu Lys Glu Arg Gln Ile Lys Lys Gln Thr Ala 
        515                 520                 525             


Leu Val Glu Leu Val Lys His Lys Pro Lys Ala Thr Lys Glu Gln Leu 
    530                 535                 540                 


Lys Ala Val Met Asp Asp Phe Ala Ala Phe Val Glu Lys Cys Cys Lys 
545                 550                 555                 560 


Ala Asp Asp Lys Glu Thr Cys Phe Ala Glu Glu Gly Lys Lys Leu Val 
                565                 570                 575     


Ala Ala Ser Gln Ala Ala Leu Gly Leu His Ser Ser Lys Leu Gln Ala 
            580                 585                 590         


Glu Pro Val Tyr Pro Asp Gln Leu Arg Leu Phe Ser Leu Gly Gln Gly 
        595                 600                 605             


Val Cys Gly Asp Lys Tyr Arg Pro Val Asn Arg Glu Glu Ala Gln Ser 
    610                 615                 620                 


Val Lys Ser Asn Ile Val Gly Met Met Gly Gln Trp Gln Ile Ser Gly 
625                 630                 635                 640 


Leu Ala Asn Gly Trp Val Ile Met Gly Pro Gly Tyr Asn Gly Glu Ile 
                645                 650                 655     


Lys Pro Gly Thr Ala Ser Asn Thr Trp Cys Tyr Pro Thr Asn Pro Val 
            660                 665                 670         


Thr Gly Glu Ile Pro Thr Leu Ser Ala Leu Asp Ile Pro Asp Gly Asp 
        675                 680                 685             


Glu Val Asp Val Gln Trp Arg Leu Val His Asp Ser Ala Asn Phe Ile 
    690                 695                 700                 


Lys Pro Thr Ser Tyr Leu Ala His Tyr Leu Gly Tyr Ala Trp Val Gly 
705                 710                 715                 720 


Gly Asn His Ser Gln Tyr Val Gly Glu Asp Met Asp Val Thr Arg Asp 
                725                 730                 735     


Gly Asp Gly Trp Val Ile Arg Gly Asn Asn Asp Gly Gly Cys Asp Gly 
            740                 745                 750         


Tyr Arg Cys Gly Asp Lys Thr Ala Ile Lys Val Ser Asn Phe Ala Tyr 
        755                 760                 765             


Asn Leu Asp Pro Asp Ser Phe Lys His Gly Asp Val Thr Gln Ser Asp 
    770                 775                 780                 


Arg Gln Leu Val Lys Thr Val Val Gly Trp Ala Val Asn Asp Ser Asp 
785                 790                 795                 800 


Thr Pro Gln Ser Gly Tyr Asp Val Thr Leu Arg Tyr Asp Thr Ala Thr 
                805                 810                 815     


Asn Trp Ser Lys Thr Asn Thr Tyr Gly Leu Ser Glu Lys Val Thr Thr 
            820                 825                 830         


Lys Asn Lys Phe Lys Trp Pro Leu Val Gly Glu Thr Gln Leu Ser Ile 
        835                 840                 845             


Glu Ile Ala Ala Asn Gln Ser Trp Ala Ser Gln Asn Gly Gly Ser Thr 
    850                 855                 860                 


Thr Thr Ser Leu Ser Gln Ser Val Arg Pro Thr Val Pro Ala Arg Ser 
865                 870                 875                 880 


Lys Ile Pro Val Lys Ile Glu Leu Tyr Lys Ala Asp Ile Ser Tyr Pro 
                885                 890                 895     


Tyr Glu Phe Lys Ala Asp Val Ser Tyr Asp Leu Thr Leu Ser Gly Phe 
            900                 905                 910         


Leu Arg Trp Gly Gly Asn Ala Trp Tyr Thr His Pro Asp Asn Arg Pro 
        915                 920                 925             


Asn Trp Asn His Thr Phe Val Ile Gly Pro Tyr Lys Asp Lys Ala Ser 
    930                 935                 940                 


Ser Ile Arg Tyr Gln Trp Asp Lys Arg Tyr Ile Pro Gly Glu Val Lys 
945                 950                 955                 960 


Trp Trp Asp Trp Asn Trp Thr Ile Gln Gln Asn Gly Leu Ser Thr Met 
                965                 970                 975     


Gln Asn Asn Leu Ala Arg Val Leu Arg Pro Val Arg Ala Gly Ile Thr 
            980                 985                 990         


Gly Asp Phe Ser Ala Glu Ser Gln  Phe Ala Gly Asn Ile  Glu Ile Gly 
        995                 1000                 1005             


Ala Pro  Val Pro Leu Ala Ala  Asp Ser His Ser Ser  Lys Leu Gln 
    1010                 1015                 1020             


Ser Val  Asp Gly Ala Gly Gln  Gly Leu Arg Leu Glu  Ile Pro Leu 
    1025                 1030                 1035             


Asp Ala  Gln Glu Leu Ser Gly  Leu Gly Phe Asn Asn  Val Ser Leu 
    1040                 1045                 1050             


Ser Val  Thr Pro Ala Ala Asn  Gln 
    1055                 1060     


