                         SEQUENCE LISTING

<110>  Agency for Science Technology and Research
 
<120>  MONOCLONAL ANTIBODY AGAINST MURAMYL PEPTIDES IN PREVENTION AND 
       TREATMENT OF IMMUNE-MEDIATED DISEASES

<130>  9869SG3533

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  624
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding for heavy chain of the variable region of 2E7

<400>  1
atgctggtgg agtctggggg aggcttggtg caacctggag gatccatgaa actctcctgt       60

atagtctcgg gatttacttt cagttattat tggatgtctt gggtccgcca gtctccagag      120

aaggggtttg agtgggttgc tgaaatcaga ttgaaatctg agaattatgc aacaaattat      180

acggagtctg tgaaagggaa gttcaccatc tcaagagatg attccaaaag tcgtctctac      240

ctgcaaatga acagcttagg agctgaggac actggaattt attactgtct aactggttat      300

gcctggtttg cttattgggg ccaagggact ctagtcactg tctctgcagc caaaacgaca      360

cccccatctg tctatccact ggcccctgga tctgctgccc aaactaactc catggtgacc      420

ctgggatgcc tggtcaaggg ctatttccct gagccagtga cagtgacctg gaactctgga      480

tccctgtcca gcggtgtgca caccttccca gctgtcctgc agtctgacct ctacactctg      540

agcagctcag tgactgtccc ctccagcacc tggcccagcg agaccgtcac ctgcaacgtt      600

gcccacccgg ccagcagcac caag                                             624


<210>  2
<211>  531
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding for light chain of the variable region of 2E7

<400>  2
gacgtccaga tgatccagtc tccaaagcgc ctaatctatc tggtgtctaa actggactct       60

ggagtccctg acaggttcac tggcagtgga tcaggaacag attttacact gaaaatcagc      120

agagtggagg ctgaggattt gggagtttat tactgcgtgc aacatacaca ttttcccacg      180

ttcggagggg ggaccaagct ggaaataaaa cgggctgatg ctgcaccaac tgtatccatc      240

ttcccaccat ccagtgagca gttaacatct ggaggtgcct cagtcgtgtg cttcttgaac      300

aacttctacc ccaaagacat caatgtcaag tggaagattg atggcagtga acgacaaaat      360

ggcgtcctga acagttggac tgatcaggac agcaaagaca gcacctacag catgagcagc      420

accctcacgt tgaccaagga cgagtatgaa cgacataaca gctatacctg tgaggccact      480

cacaagacat caacttcacc cattgtcaag agcttcaaca ggaatgagtg t               531


<210>  3
<211>  208
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Heavy chain of the variable region of 2E7

<400>  3

Met Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Met 
1               5                   10                  15      


Lys Leu Ser Cys Ile Val Ser Gly Phe Thr Phe Ser Tyr Tyr Trp Met 
            20                  25                  30          


Ser Trp Val Arg Gln Ser Pro Glu Lys Gly Phe Glu Trp Val Ala Glu 
        35                  40                  45              


Ile Arg Leu Lys Ser Glu Asn Tyr Ala Thr Asn Tyr Thr Glu Ser Val 
    50                  55                  60                  


Lys Gly Lys Phe Thr Ile Ser Arg Asp Asp Ser Lys Ser Arg Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Gly Ala Glu Asp Thr Gly Ile Tyr Tyr Cys 
                85                  90                  95      


Leu Thr Gly Tyr Ala Trp Phe Ala Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ala Ala Lys Thr Thr Pro Pro Ser Val Tyr Pro Leu Ala 
        115                 120                 125             


Pro Gly Ser Ala Ala Gln Thr Asn Ser Met Val Thr Leu Gly Cys Leu 
    130                 135                 140                 


Val Lys Gly Tyr Phe Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly 
145                 150                 155                 160 


Ser Leu Ser Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Asp 
                165                 170                 175     


Leu Tyr Thr Leu Ser Ser Ser Val Thr Val Pro Ser Ser Thr Trp Pro 
            180                 185                 190         


Ser Glu Thr Val Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys 
        195                 200                 205             


<210>  4
<211>  177
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Light chain of the variable region of 2E7

<400>  4

Asp Val Gln Met Ile Gln Ser Pro Lys Arg Leu Ile Tyr Leu Val Ser 
1               5                   10                  15      


Lys Leu Asp Ser Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly 
            20                  25                  30          


Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly 
        35                  40                  45              


Val Tyr Tyr Cys Val Gln His Thr His Phe Pro Thr Phe Gly Gly Gly 
    50                  55                  60                  


Thr Lys Leu Glu Ile Lys Arg Ala Asp Ala Ala Pro Thr Val Ser Ile 
65                  70                  75                  80  


Phe Pro Pro Ser Ser Glu Gln Leu Thr Ser Gly Gly Ala Ser Val Val 
                85                  90                  95      


Cys Phe Leu Asn Asn Phe Tyr Pro Lys Asp Ile Asn Val Lys Trp Lys 
            100                 105                 110         


Ile Asp Gly Ser Glu Arg Gln Asn Gly Val Leu Asn Ser Trp Thr Asp 
        115                 120                 125             


Gln Asp Ser Lys Asp Ser Thr Tyr Ser Met Ser Ser Thr Leu Thr Leu 
    130                 135                 140                 


Thr Lys Asp Glu Tyr Glu Arg His Asn Ser Tyr Thr Cys Glu Ala Thr 
145                 150                 155                 160 


His Lys Thr Ser Thr Ser Pro Ile Val Lys Ser Phe Asn Arg Asn Glu 
                165                 170                 175     


Cys 
    


<210>  5
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for nucleotide sequence encoding for heavy chain of 
       the variable region of 2E7

<400>  5
atgctggtgg agtctggggg a                                                 21


<210>  6
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for nucleotide sequence encoding for heavy chain of 
       the variable region of 2E7

<400>  6
aagctggtgg aatctggagg a                                                 21


<210>  7
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for nucleotide sequence encoding for heavy chain of 
       the variable region of 2E7

<400>  7
cttggtgctg ctggccgggt g                                                 21


<210>  8
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for nucleotide sequence encoding for light chain of 
       the variable region  of 2E7

<400>  8
ccgtttgatt tccagcttgg tgcc                                              24


<210>  9
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for nucleotide sequence encoding for light chain of 
       the variable region of 2E7

<400>  9
ccgtttcagc tccagcttgg tccc                                              24


<210>  10
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for nucleotide sequence encoding for a light chain of 
       the variable region of 2E7

<400>  10
gacattgagc tcacccagtc tcca                                              24


