                         SEQUENCE LISTING

<110>  Pieris AG
 
<120>  Engineered T cells and uses therefor

<130>  PIE15249PCT

<150>  EP 15 000 047.9
<151>  2015-01-12

<160>  29    

<170>  PatentIn version 3.5

<210>  1
<211>  152
<212>  PRT
<213>  artificial

<220>
<223>  Lipocalin mutein binding c-Met

<400>  1

Ala Ser Asp Glu Glu Ile Gln Asp Val Ser Gly Thr Trp Tyr Leu Lys 
1               5                   10                  15      


Ala Met Thr Val Asp Thr Gln Asp Pro Leu Ser Leu Tyr Val Ser Val 
            20                  25                  30          


Ser Pro Ile Thr Leu Thr Thr Leu Glu Gly Gly Asn Leu Glu Ala Thr 
        35                  40                  45              


Val Thr Leu Asn Gln Ile Gly Arg Ser Gln Glu Val Leu Ala Val Leu 
    50                  55                  60                  


Glu Lys Thr Asp Glu Pro Gly Lys Tyr Thr Leu Tyr Gly Gly Ala His 
65                  70                  75                  80  


Val Ala Tyr Ile Gln Arg Ser His Val Lys Asp His Tyr Ile Phe Tyr 
                85                  90                  95      


Ser Glu Gly Asp Thr Trp Gly Gly Pro Val Pro Gly Val Trp Leu Val 
            100                 105                 110         


Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala Leu Glu Asp Phe Glu Lys 
        115                 120                 125             


Ala Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser Ile Leu Ile Pro Arg 
    130                 135                 140                 


Gln Ser Glu Thr Ser Ser Pro Gly 
145                 150         


<210>  2
<211>  178
<212>  PRT
<213>  artificial

<220>
<223>  Lipocalin mutein binding GPC-3

<400>  2

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Arg Ala Gly Asn Val Ala Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              


Pro Lys Met Arg Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  


Asn Val Thr Asn Val Arg Phe Ala Met Lys Lys Cys Met Tyr Ser Ile 
65                  70                  75                  80  


Gly Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Gln 
                85                  90                  95      


Ile Lys Ser Glu Pro Gly Asn Thr Ser Asn Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Glu Val Tyr Gln 
        115                 120                 125             


Asn Arg Glu Ile Phe Phe Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 


Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 


Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     


Asp Gly 
        


<210>  3
<211>  178
<212>  PRT
<213>  artificial

<220>
<223>  Lipocalin mutein binding CTLA-4

<400>  3

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe Gln Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Leu Ala Gly Asn Arg Ile Leu Arg Gln Asp Gln His Pro 
        35                  40                  45              


Met Leu Met Tyr Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  


Gln Val Thr Ser Val Ile Ser Ser His Lys Lys Cys Leu Tyr Pro Ile 
65                  70                  75                  80  


Ala Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 
                85                  90                  95      


Ile Lys Ser Tyr Gly Asp Lys Val Ser Tyr Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys His Ala Asp Thr 
        115                 120                 125             


Asn Tyr Glu Ser Phe Ser Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 


Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 


Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     


Asp Gly 
        


<210>  4
<211>  69
<212>  PRT
<213>  artificial

<220>
<223>  CD8 linker and transmembrane region

<400>  4

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile 
        35                  40                  45              


Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val 
    50                  55                  60                  


Ile Thr Leu Tyr Cys 
65                  


<210>  5
<211>  113
<212>  PRT
<213>  artificial

<220>
<223>  intracellular signaling domain of CD3zeta

<400>  5

Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln 
1               5                   10                  15      


Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu 
            20                  25                  30          


Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly 
        35                  40                  45              


Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
    50                  55                  60                  


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
65                  70                  75                  80  


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
                85                  90                  95      


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
            100                 105                 110         


Arg 
    


<210>  6
<211>  44
<212>  PRT
<213>  artificial

<220>
<223>  intracellular signaling domain of CD28

<400>  6

Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met 
1               5                   10                  15      


Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro 
            20                  25                  30          


Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40                  


<210>  7
<211>  41
<212>  PRT
<213>  artificial

<220>
<223>  intracellular signaling domain of CD137

<400>  7

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu 
        35                  40      


<210>  8
<211>  378
<212>  PRT
<213>  artificial

<220>
<223>  c-Met-CD28-CD3zeta polypeptide

<400>  8

Ala Ser Asp Glu Glu Ile Gln Asp Val Ser Gly Thr Trp Tyr Leu Lys 
1               5                   10                  15      


Ala Met Thr Val Asp Thr Gln Asp Pro Leu Ser Leu Tyr Val Ser Val 
            20                  25                  30          


Ser Pro Ile Thr Leu Thr Thr Leu Glu Gly Gly Asn Leu Glu Ala Thr 
        35                  40                  45              


Val Thr Leu Asn Gln Ile Gly Arg Ser Gln Glu Val Leu Ala Val Leu 
    50                  55                  60                  


Glu Lys Thr Asp Glu Pro Gly Lys Tyr Thr Leu Tyr Gly Gly Ala His 
65                  70                  75                  80  


Val Ala Tyr Ile Gln Arg Ser His Val Lys Asp His Tyr Ile Phe Tyr 
                85                  90                  95      


Ser Glu Gly Asp Thr Trp Gly Gly Pro Val Pro Gly Val Trp Leu Val 
            100                 105                 110         


Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala Leu Glu Asp Phe Glu Lys 
        115                 120                 125             


Ala Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser Ile Leu Ile Pro Arg 
    130                 135                 140                 


Gln Ser Glu Thr Ser Ser Pro Gly Thr Thr Thr Pro Ala Pro Arg Pro 
145                 150                 155                 160 


Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro 
                165                 170                 175     


Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu 
            180                 185                 190         


Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys 
        195                 200                 205             


Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Phe Trp Val 
    210                 215                 220                 


Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
225                 230                 235                 240 


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
                245                 250                 255     


Pro Arg Asp Phe Ala Ala Tyr Arg Ser Leu Arg Val Lys Phe Ser Arg 
            260                 265                 270         


Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln Asn Gln Leu Tyr Asn 
        275                 280                 285             


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
    290                 295                 300                 


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
305                 310                 315                 320 


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
                325                 330                 335     


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
            340                 345                 350         


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
        355                 360                 365             


Ala Leu His Met Gln Ala Leu Pro Pro Arg 
    370                 375             


<210>  9
<211>  375
<212>  PRT
<213>  artificial

<220>
<223>  c-Met-CD137-CD3zeta polypeptide

<400>  9

Ala Ser Asp Glu Glu Ile Gln Asp Val Ser Gly Thr Trp Tyr Leu Lys 
1               5                   10                  15      


Ala Met Thr Val Asp Thr Gln Asp Pro Leu Ser Leu Tyr Val Ser Val 
            20                  25                  30          


Ser Pro Ile Thr Leu Thr Thr Leu Glu Gly Gly Asn Leu Glu Ala Thr 
        35                  40                  45              


Val Thr Leu Asn Gln Ile Gly Arg Ser Gln Glu Val Leu Ala Val Leu 
    50                  55                  60                  


Glu Lys Thr Asp Glu Pro Gly Lys Tyr Thr Leu Tyr Gly Gly Ala His 
65                  70                  75                  80  


Val Ala Tyr Ile Gln Arg Ser His Val Lys Asp His Tyr Ile Phe Tyr 
                85                  90                  95      


Ser Glu Gly Asp Thr Trp Gly Gly Pro Val Pro Gly Val Trp Leu Val 
            100                 105                 110         


Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala Leu Glu Asp Phe Glu Lys 
        115                 120                 125             


Ala Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser Ile Leu Ile Pro Arg 
    130                 135                 140                 


Gln Ser Glu Thr Ser Ser Pro Gly Thr Thr Thr Pro Ala Pro Arg Pro 
145                 150                 155                 160 


Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro 
                165                 170                 175     


Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu 
            180                 185                 190         


Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys 
        195                 200                 205             


Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg Gly 
    210                 215                 220                 


Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val 
225                 230                 235                 240 


Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu 
                245                 250                 255     


Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp 
            260                 265                 270         


Ala Pro Ala Tyr Lys Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn 
        275                 280                 285             


Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg 
    290                 295                 300                 


Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly 
305                 310                 315                 320 


Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu 
                325                 330                 335     


Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu 
            340                 345                 350         


Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His 
        355                 360                 365             


Met Gln Ala Leu Pro Pro Arg 
    370                 375 


<210>  10
<211>  404
<212>  PRT
<213>  artificial

<220>
<223>  GPC3-CD28-CD3zeta polypeptide

<400>  10

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Arg Ala Gly Asn Val Ala Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              


Pro Lys Met Arg Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  


Asn Val Thr Asn Val Arg Phe Ala Met Lys Lys Cys Met Tyr Ser Ile 
65                  70                  75                  80  


Gly Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Gln 
                85                  90                  95      


Ile Lys Ser Glu Pro Gly Asn Thr Ser Asn Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Glu Val Tyr Gln 
        115                 120                 125             


Asn Arg Glu Ile Phe Phe Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 


Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 


Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     


Asp Gly Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr 
            180                 185                 190         


Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala 
        195                 200                 205             


Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile 
    210                 215                 220                 


Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser 
225                 230                 235                 240 


Leu Val Ile Thr Leu Tyr Cys Phe Trp Val Arg Ser Lys Arg Ser Arg 
                245                 250                 255     


Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro 
            260                 265                 270         


Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala 
        275                 280                 285             


Tyr Arg Ser Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala 
    290                 295                 300                 


Tyr Lys Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg 
305                 310                 315                 320 


Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu 
                325                 330                 335     


Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn 
            340                 345                 350         


Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
        355                 360                 365             


Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly 
    370                 375                 380                 


Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala 
385                 390                 395                 400 


Leu Pro Pro Arg 
                


<210>  11
<211>  401
<212>  PRT
<213>  artificial

<220>
<223>  GPC3-CD137-CD3zeta polypeptide

<400>  11

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Arg Ala Gly Asn Val Ala Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              


Pro Lys Met Arg Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  


Asn Val Thr Asn Val Arg Phe Ala Met Lys Lys Cys Met Tyr Ser Ile 
65                  70                  75                  80  


Gly Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Gln 
                85                  90                  95      


Ile Lys Ser Glu Pro Gly Asn Thr Ser Asn Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Glu Val Tyr Gln 
        115                 120                 125             


Asn Arg Glu Ile Phe Phe Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 


Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 


Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     


Asp Gly Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr 
            180                 185                 190         


Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala 
        195                 200                 205             


Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile 
    210                 215                 220                 


Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser 
225                 230                 235                 240 


Leu Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr 
                245                 250                 255     


Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu 
            260                 265                 270         


Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu 
        275                 280                 285             


Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln 
    290                 295                 300                 


Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu 
305                 310                 315                 320 


Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly 
                325                 330                 335     


Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
            340                 345                 350         


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
        355                 360                 365             


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
    370                 375                 380                 


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
385                 390                 395                 400 


Arg 
    


<210>  12
<211>  456
<212>  DNA
<213>  artificial

<220>
<223>  nucleotide sequence encoding amino acid sequence of SEQ ID NO: 1

<400>  12
gcttctgatg aagaaatcca ggatgtgtct ggcacctggt acctgaaggc tatgaccgtg       60

gatacccagg atccgctgtc tctgtacgtg tctgtgtctc cgatcaccct gaccaccctg      120

gaaggcggca acctggaagc taccgtgacc ctgaaccaga tcggccgttc tcaggaagtg      180

ctggctgtgc tggaaaagac cgatgaaccg ggcaagtaca ccctgtacgg cggcgctcat      240

gtggcttaca tccagcgttc tcatgtgaag gatcattaca tcttctactc tgaaggcgat      300

acctggggcg gcccggtgcc gggcgtgtgg ctggtgggcc gtgatccgaa gaacaacctg      360

gaagctctgg aagatttcga aaaggctgct ggcgctcgtg gcctgtctac cgaatctatc      420

ctgatcccgc gtcagtctga aacctcttct ccgggc                                456


<210>  13
<211>  534
<212>  DNA
<213>  artificial

<220>
<223>  nucleotide sequence encoding amino acid sequence of SEQ ID NO: 2

<400>  13
caggattcta cctctgatct gatcccggct ccgccgctgt ctaaggtgcc gctgcagcag       60

aacttccagg ataaccagtt ccatggcaag tggtacgtgg tgggccgtgc tggcaacgtg      120

gctctgcgtg aagataagga tccgccgaag atgcgtgcta ccatctacga actgaaggaa      180

gataagtctt acaacgtgac caacgtgcgt ttcgctatga agaagtgcat gtactctatc      240

ggcaccttcg tgccgggctc tcagccgggc gaattcaccc tgggccagat caagtctgaa      300

ccgggcaaca cctctaacct ggtgcgtgtg gtgtctacca actacaacca gcatgctatg      360

gtgttcttca aggaagtgta ccagaaccgt gaaatcttct tcatcaccct gtacggccgt      420

accaaggaac tgacctctga actgaaggaa aacttcatcc gtttctctaa gtctctgggc      480

ctgccggaaa accatatcgt gttcccggtg ccgatcgatc agtgcatcga tggc            534


<210>  14
<211>  534
<212>  DNA
<213>  artificial

<220>
<223>  nucleotide sequence encoding amino acid sequence of SEQ ID NO: 3

<400>  14
caggattcta cctctgatct gatcccggct ccgccgctgt ctaaggtgcc gctgcagcag       60

aacttccagg ataaccagtt ccagggcaag tggtacgtgg tgggcctggc tggcaaccgt      120

atcctgcgtc aggatcagca tccgatgctg atgtacgcta ccatctacga actgaaggaa      180

gataagtctt accaggtgac ctctgtgatc tcttctcata agaagtgcct gtacccgatc      240

gctaccttcg tgccgggctc tcagccgggc gaattcaccc tgggcaacat caagtcttac      300

ggcgataagg tgtcttacct ggtgcgtgtg gtgtctacca actacaacca gcatgctatg      360

gtgttcttca agcatgctga taccaactac gaatctttct ctatcaccct gtacggccgt      420

accaaggaac tgacctctga actgaaggaa aacttcatcc gtttctctaa gtctctgggc      480

ctgccggaaa accatatcgt gttcccggtg ccgatcgatc agtgcatcga tggc            534


<210>  15
<211>  207
<212>  DNA
<213>  artificial

<220>
<223>  nucleotide sequence encoding amino acid sequence of SEQ ID NO: 4

<400>  15
accaccaccc cggctccgcg tccgccgacc ccggctccga ccatcgcttc tcagccgctg       60

tctctgcgtc cggaagcttg ccgtccggct gctggcggcg ctgtgcatac ccgtggcctg      120

gatttcgctt gcgatatcta catctgggct ccgctggctg gcacctgcgg cgtgctgctg      180

ctgtctctgg tgatcaccct gtactgc                                          207


<210>  16
<211>  339
<212>  DNA
<213>  artificial

<220>
<223>  nucleotide sequence encoding amino acid sequence of SEQ ID NO: 5

<400>  16
ctgcgtgtga agttctctcg ttctgctgat gctccggctt acaagcaggg ccagaaccag       60

ctgtacaacg aactgaacct gggccgtcgt gaagaatacg atgtgctgga taagcgtcgt      120

ggccgtgatc cggaaatggg cggcaagccg cgtcgtaaga acccgcagga aggcctgtac      180

aacgaactgc agaaggataa gatggctgaa gcttactctg aaatcggcat gaagggcgaa      240

cgtcgtcgtg gcaagggcca tgatggcctg taccagggcc tgtctaccgc taccaaggat      300

acctacgatg ctctgcatat gcaggctctg ccgccgcgt                             339


<210>  17
<211>  132
<212>  DNA
<213>  artificial

<220>
<223>  nucleotide sequence encoding amino acid sequence of SEQ ID NO: 6

<400>  17
ttctgggtgc gttctaagcg ttctcgtctg ctgcattctg attacatgaa catgaccccg       60

cgtcgtccgg gcccgacccg taagcattac cagccgtacg ctccgccgcg tgatttcgct      120

gcttaccgtt ct                                                          132


<210>  18
<211>  123
<212>  DNA
<213>  artificial

<220>
<223>  nucleotide sequence encoding amino acid sequence of SEQ ID NO: 7

<400>  18
aagcgtggcc gtaagaagct gctgtacatc ttcaagcagc cgttcatgcg tccggtgcag       60

accacccagg aagaagatgg ctgctcttgc cgtttcccgg aagaagaaga aggcggctgc      120

gaa                                                                    123


<210>  19
<211>  1134
<212>  DNA
<213>  artificial

<220>
<223>  nucleotide sequence encoding amino acid sequence of SEQ ID NO: 8

<400>  19
gcttctgatg aagaaatcca ggatgtgtct ggcacctggt acctgaaggc tatgaccgtg       60

gatacccagg atccgctgtc tctgtacgtg tctgtgtctc cgatcaccct gaccaccctg      120

gaaggcggca acctggaagc taccgtgacc ctgaaccaga tcggccgttc tcaggaagtg      180

ctggctgtgc tggaaaagac cgatgaaccg ggcaagtaca ccctgtacgg cggcgctcat      240

gtggcttaca tccagcgttc tcatgtgaag gatcattaca tcttctactc tgaaggcgat      300

acctggggcg gcccggtgcc gggcgtgtgg ctggtgggcc gtgatccgaa gaacaacctg      360

gaagctctgg aagatttcga aaaggctgct ggcgctcgtg gcctgtctac cgaatctatc      420

ctgatcccgc gtcagtctga aacctcttct ccgggcacca ccaccccggc tccgcgtccg      480

ccgaccccgg ctccgaccat cgcttctcag ccgctgtctc tgcgtccgga agcttgccgt      540

ccggctgctg gcggcgctgt gcatacccgt ggcctggatt tcgcttgcga tatctacatc      600

tgggctccgc tggctggcac ctgcggcgtg ctgctgctgt ctctggtgat caccctgtac      660

tgcttctggg tgcgttctaa gcgttctcgt ctgctgcatt ctgattacat gaacatgacc      720

ccgcgtcgtc cgggcccgac ccgtaagcat taccagccgt acgctccgcc gcgtgatttc      780

gctgcttacc gttctctgcg tgtgaagttc tctcgttctg ctgatgctcc ggcttacaag      840

cagggccaga accagctgta caacgaactg aacctgggcc gtcgtgaaga atacgatgtg      900

ctggataagc gtcgtggccg tgatccggaa atgggcggca agccgcgtcg taagaacccg      960

caggaaggcc tgtacaacga actgcagaag gataagatgg ctgaagctta ctctgaaatc     1020

ggcatgaagg gcgaacgtcg tcgtggcaag ggccatgatg gcctgtacca gggcctgtct     1080

accgctacca aggataccta cgatgctctg catatgcagg ctctgccgcc gcgt           1134


<210>  20
<211>  1125
<212>  DNA
<213>  artificial

<220>
<223>  nucleotide sequence encoding amino acid sequence of SEQ ID NO: 9

<400>  20
gcttctgatg aagaaatcca ggatgtgtct ggcacctggt acctgaaggc tatgaccgtg       60

gatacccagg atccgctgtc tctgtacgtg tctgtgtctc cgatcaccct gaccaccctg      120

gaaggcggca acctggaagc taccgtgacc ctgaaccaga tcggccgttc tcaggaagtg      180

ctggctgtgc tggaaaagac cgatgaaccg ggcaagtaca ccctgtacgg cggcgctcat      240

gtggcttaca tccagcgttc tcatgtgaag gatcattaca tcttctactc tgaaggcgat      300

acctggggcg gcccggtgcc gggcgtgtgg ctggtgggcc gtgatccgaa gaacaacctg      360

gaagctctgg aagatttcga aaaggctgct ggcgctcgtg gcctgtctac cgaatctatc      420

ctgatcccgc gtcagtctga aacctcttct ccgggcacca ccaccccggc tccgcgtccg      480

ccgaccccgg ctccgaccat cgcttctcag ccgctgtctc tgcgtccgga agcttgccgt      540

ccggctgctg gcggcgctgt gcatacccgt ggcctggatt tcgcttgcga tatctacatc      600

tgggctccgc tggctggcac ctgcggcgtg ctgctgctgt ctctggtgat caccctgtac      660

tgcaagcgtg gccgtaagaa gctgctgtac atcttcaagc agccgttcat gcgtccggtg      720

cagaccaccc aggaagaaga tggctgctct tgccgtttcc cggaagaaga agaaggcggc      780

tgcgaactgc gtgtgaagtt ctctcgttct gctgatgctc cggcttacaa gcagggccag      840

aaccagctgt acaacgaact gaacctgggc cgtcgtgaag aatacgatgt gctggataag      900

cgtcgtggcc gtgatccgga aatgggcggc aagccgcgtc gtaagaaccc gcaggaaggc      960

ctgtacaacg aactgcagaa ggataagatg gctgaagctt actctgaaat cggcatgaag     1020

ggcgaacgtc gtcgtggcaa gggccatgat ggcctgtacc agggcctgtc taccgctacc     1080

aaggatacct acgatgctct gcatatgcag gctctgccgc cgcgt                     1125


<210>  21
<211>  1212
<212>  DNA
<213>  artificial

<220>
<223>  nucleotide sequence encoding amino acid sequence of SEQ ID NO: 10

<400>  21
caggattcta cctctgatct gatcccggct ccgccgctgt ctaaggtgcc gctgcagcag       60

aacttccagg ataaccagtt ccatggcaag tggtacgtgg tgggccgtgc tggcaacgtg      120

gctctgcgtg aagataagga tccgccgaag atgcgtgcta ccatctacga actgaaggaa      180

gataagtctt acaacgtgac caacgtgcgt ttcgctatga agaagtgcat gtactctatc      240

ggcaccttcg tgccgggctc tcagccgggc gaattcaccc tgggccagat caagtctgaa      300

ccgggcaaca cctctaacct ggtgcgtgtg gtgtctacca actacaacca gcatgctatg      360

gtgttcttca aggaagtgta ccagaaccgt gaaatcttct tcatcaccct gtacggccgt      420

accaaggaac tgacctctga actgaaggaa aacttcatcc gtttctctaa gtctctgggc      480

ctgccggaaa accatatcgt gttcccggtg ccgatcgatc agtgcatcga tggcaccacc      540

accccggctc cgcgtccgcc gaccccggct ccgaccatcg cttctcagcc gctgtctctg      600

cgtccggaag cttgccgtcc ggctgctggc ggcgctgtgc atacccgtgg cctggatttc      660

gcttgcgata tctacatctg ggctccgctg gctggcacct gcggcgtgct gctgctgtct      720

ctggtgatca ccctgtactg cttctgggtg cgttctaagc gttctcgtct gctgcattct      780

gattacatga acatgacccc gcgtcgtccg ggcccgaccc gtaagcatta ccagccgtac      840

gctccgccgc gtgatttcgc tgcttaccgt tctctgcgtg tgaagttctc tcgttctgct      900

gatgctccgg cttacaagca gggccagaac cagctgtaca acgaactgaa cctgggccgt      960

cgtgaagaat acgatgtgct ggataagcgt cgtggccgtg atccggaaat gggcggcaag     1020

ccgcgtcgta agaacccgca ggaaggcctg tacaacgaac tgcagaagga taagatggct     1080

gaagcttact ctgaaatcgg catgaagggc gaacgtcgtc gtggcaaggg ccatgatggc     1140

ctgtaccagg gcctgtctac cgctaccaag gatacctacg atgctctgca tatgcaggct     1200

ctgccgccgc gt                                                         1212


<210>  22
<211>  1203
<212>  DNA
<213>  artificial

<220>
<223>  nucleotide sequence encoding amino acid sequence of SEQ ID NO: 11

<400>  22
caggattcta cctctgatct gatcccggct ccgccgctgt ctaaggtgcc gctgcagcag       60

aacttccagg ataaccagtt ccatggcaag tggtacgtgg tgggccgtgc tggcaacgtg      120

gctctgcgtg aagataagga tccgccgaag atgcgtgcta ccatctacga actgaaggaa      180

gataagtctt acaacgtgac caacgtgcgt ttcgctatga agaagtgcat gtactctatc      240

ggcaccttcg tgccgggctc tcagccgggc gaattcaccc tgggccagat caagtctgaa      300

ccgggcaaca cctctaacct ggtgcgtgtg gtgtctacca actacaacca gcatgctatg      360

gtgttcttca aggaagtgta ccagaaccgt gaaatcttct tcatcaccct gtacggccgt      420

accaaggaac tgacctctga actgaaggaa aacttcatcc gtttctctaa gtctctgggc      480

ctgccggaaa accatatcgt gttcccggtg ccgatcgatc agtgcatcga tggcaccacc      540

accccggctc cgcgtccgcc gaccccggct ccgaccatcg cttctcagcc gctgtctctg      600

cgtccggaag cttgccgtcc ggctgctggc ggcgctgtgc atacccgtgg cctggatttc      660

gcttgcgata tctacatctg ggctccgctg gctggcacct gcggcgtgct gctgctgtct      720

ctggtgatca ccctgtactg caagcgtggc cgtaagaagc tgctgtacat cttcaagcag      780

ccgttcatgc gtccggtgca gaccacccag gaagaagatg gctgctcttg ccgtttcccg      840

gaagaagaag aaggcggctg cgaactgcgt gtgaagttct ctcgttctgc tgatgctccg      900

gcttacaagc agggccagaa ccagctgtac aacgaactga acctgggccg tcgtgaagaa      960

tacgatgtgc tggataagcg tcgtggccgt gatccggaaa tgggcggcaa gccgcgtcgt     1020

aagaacccgc aggaaggcct gtacaacgaa ctgcagaagg ataagatggc tgaagcttac     1080

tctgaaatcg gcatgaaggg cgaacgtcgt cgtggcaagg gccatgatgg cctgtaccag     1140

ggcctgtcta ccgctaccaa ggatacctac gatgctctgc atatgcaggc tctgccgccg     1200

cgt                                                                   1203


<210>  23
<211>  209
<212>  PRT
<213>  artificial

<220>
<223>  secreted anti-CTLA-4 antagonist

<400>  23

Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile Ser Ala Ser 
1               5                   10                  15      


Val Ile Met Ser Arg Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro 
            20                  25                  30          


Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe 
        35                  40                  45              


Gln Gly Lys Trp Tyr Val Val Gly Leu Ala Gly Asn Arg Ile Leu Arg 
    50                  55                  60                  


Gln Asp Gln His Pro Met Leu Met Tyr Ala Thr Ile Tyr Glu Leu Lys 
65                  70                  75                  80  


Glu Asp Lys Ser Tyr Gln Val Thr Ser Val Ile Ser Ser His Lys Lys 
                85                  90                  95      


Cys Leu Tyr Pro Ile Ala Thr Phe Val Pro Gly Ser Gln Pro Gly Glu 
            100                 105                 110         


Phe Thr Leu Gly Asn Ile Lys Ser Tyr Gly Asp Lys Val Ser Tyr Leu 
        115                 120                 125             


Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe 
    130                 135                 140                 


Lys His Ala Asp Thr Asn Tyr Glu Ser Phe Ser Ile Thr Leu Tyr Gly 
145                 150                 155                 160 


Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe 
                165                 170                 175     


Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro 
            180                 185                 190         


Ile Asp Gln Cys Ile Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu 
        195                 200                 205             


Lys 
    


<210>  24
<211>  630
<212>  DNA
<213>  artificial

<220>
<223>  nucleotide sequence encoding the amino acid sequence of SEQ ID 
       NO: 23

<400>  24
atggattttc aggtgcagat tttcagcttc ctgctaatca gtgcctcagt cataatgtct       60

agacaggact ccacctctga tctcatcccc gcacctccgc ttagcaaggt tcccttgcaa      120

cagaattttc aggacaatca attccagggg aaatggtacg tagtggggct cgccggcaac      180

agaatcctga gacaagacca gcacccaatg ctgatgtacg ctacgatcta cgagttgaag      240

gaagacaagt catatcaagt aacaagcgtt atttcttctc acaaaaaatg cctgtatcca      300

atcgctacat ttgtccctgg ttcccagccc ggggaattta cccttggcaa catcaagtct      360

tatggtgata aagtgtccta tctggtgaga gttgtctcta ccaattacaa tcagcacgct      420

atggtcttct tcaaacatgc cgatacaaat tacgaaagct tcagtatcac tctgtatgga      480

aggactaaag aattgactag cgagcttaaa gagaacttca tacggttcag caaaagcctg      540

gggctccccg agaaccatat tgtgtttccc gtacctatag atcagtgcat tgacggcagt      600

gcatggtctc acccccagtt cgagaaatga                                       630


<210>  25
<211>  445
<212>  PRT
<213>  artificial

<220>
<223>  secreted anti-CTLA-4 antagonist

<400>  25

Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile Ser Ala Ser 
1               5                   10                  15      


Val Ile Met Ser Arg Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro 
            20                  25                  30          


Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe 
        35                  40                  45              


Gln Gly Lys Trp Tyr Val Val Gly Leu Ala Gly Asn Arg Ile Leu Arg 
    50                  55                  60                  


Gln Asp Gln His Pro Met Leu Met Tyr Ala Thr Ile Tyr Glu Leu Lys 
65                  70                  75                  80  


Glu Asp Lys Ser Tyr Gln Val Thr Ser Val Ile Ser Ser His Lys Lys 
                85                  90                  95      


Cys Leu Tyr Pro Ile Ala Thr Phe Val Pro Gly Ser Gln Pro Gly Glu 
            100                 105                 110         


Phe Thr Leu Gly Asn Ile Lys Ser Tyr Gly Asp Lys Val Ser Tyr Leu 
        115                 120                 125             


Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe 
    130                 135                 140                 


Lys His Ala Asp Thr Asn Tyr Glu Ser Phe Ser Ile Thr Leu Tyr Gly 
145                 150                 155                 160 


Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe 
                165                 170                 175     


Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro 
            180                 185                 190         


Ile Asp Gln Cys Ile Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu 
        195                 200                 205             


Lys Asp Pro Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro 
    210                 215                 220                 


Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe 
225                 230                 235                 240 


Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val 
                245                 250                 255     


Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe 
            260                 265                 270         


Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro 
        275                 280                 285             


Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr 
    290                 295                 300                 


Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val 
305                 310                 315                 320 


Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala 
                325                 330                 335     


Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg 
            340                 345                 350         


Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly 
        355                 360                 365             


Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro 
    370                 375                 380                 


Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser 
385                 390                 395                 400 


Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln 
                405                 410                 415     


Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His 
            420                 425                 430         


Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Lys 
        435                 440                 445 


<210>  26
<211>  1338
<212>  DNA
<213>  artificial

<220>
<223>  nucleotide sequence encoding amino acid sequence of SEQ ID NO: 25

<400>  26
atggattttc aggtgcaaat cttttctttc cttctgatta gcgcttctgt gatcatgtca       60

aggcaagatt caacttccga tctgatcccg gcgccaccac tctccaaggt accactgcag      120

cagaactttc aggataacca gtttcagggg aaatggtacg tagtgggcct tgccggaaac      180

cgaatcctgc ggcaggatca acaccccatg ctgatgtacg ctaccatcta tgagctgaag      240

gaagataaga gttatcaggt gacgagcgtc atctcttccc acaaaaagtg cctgtatccc      300

attgccacat tcgtgccggg cagtcaaccg ggcgagttca ccctcgggaa catcaaatcc      360

tacggcgata aggtaagtta cttggtcagg gtcgtgtcta caaactacaa ccaacacgcc      420

atggtttttt tcaagcacgc tgatacaaac tacgagagct tctcaatcac cttgtacggt      480

cggaccaaag aactgacgtc tgagcttaag gagaatttta tacgcttctc aaagagcctg      540

gggctgccag aaaaccacat cgttttccca gtgccaatcg accagtgtat cgatggctct      600

gcctggtccc acccacagtt tgagaaagat ccagctgaac caaagagtcc agacaagact      660

cacacatgtc caccttgccc cgccccagaa ttgttgggag gcccctctgt gtttctcttc      720

cctcctaagc caaaagacac tctgatgata tcacggaccc cagaggttac ttgcgttgtc      780

gtcgatgtga gtcacgaaga tcctgaggtc aagtttaact ggtacgtaga tggggttgag      840

gtgcataacg caaagaccaa acctcgcgag gaacagtata acagtacata tcgcgtggta      900

tccgtgctca ccgtcctcca tcaagactgg ttgaatggaa aggaatacaa gtgcaaagtt      960

tctaacaaag ccctgccagc gcccatcgaa aagactatct ctaaagccaa gggccaacct     1020

cgcgaacctc aagtgtacac ccttcctccc agccgggatg aactgaccaa aaaccaagtg     1080

agcctgacat gtctggtgaa gggtttctat ccctctgata ttgcggttga atgggaaagc     1140

aacggacagc ccgaaaacaa ctacaagact acaccccccg ttttggattc cgatgggagt     1200

tttttcttgt attctaagct gaccgttgat aaaagtaggt ggcagcaggg caatgtgttt     1260

tcatgtagcg tgatgcacga ggcccttcac aaccactata cccagaagag tcttagcctc     1320

agccctggaa agaagtga                                                   1338


<210>  27
<211>  10
<212>  PRT
<213>  artificial

<220>
<223>  Strep-tag II

<400>  27

Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
1               5                   10  


<210>  28
<211>  188
<212>  PRT
<213>  artificial

<220>
<223>  secreted anti-CTLA-4 antagonist without leader sequence

<400>  28

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe Gln Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Leu Ala Gly Asn Arg Ile Leu Arg Gln Asp Gln His Pro 
        35                  40                  45              


Met Leu Met Tyr Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  


Gln Val Thr Ser Val Ile Ser Ser His Lys Lys Cys Leu Tyr Pro Ile 
65                  70                  75                  80  


Ala Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 
                85                  90                  95      


Ile Lys Ser Tyr Gly Asp Lys Val Ser Tyr Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys His Ala Asp Thr 
        115                 120                 125             


Asn Tyr Glu Ser Phe Ser Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 


Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 


Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     


Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
            180                 185             


<210>  29
<211>  424
<212>  PRT
<213>  artificial

<220>
<223>  secreted anti-CTLA-4 antagonist without leader sequence

<400>  29

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe Gln Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Leu Ala Gly Asn Arg Ile Leu Arg Gln Asp Gln His Pro 
        35                  40                  45              


Met Leu Met Tyr Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  


Gln Val Thr Ser Val Ile Ser Ser His Lys Lys Cys Leu Tyr Pro Ile 
65                  70                  75                  80  


Ala Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 
                85                  90                  95      


Ile Lys Ser Tyr Gly Asp Lys Val Ser Tyr Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys His Ala Asp Thr 
        115                 120                 125             


Asn Tyr Glu Ser Phe Ser Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 


Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 


Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     


Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys Asp Pro Ala Glu 
            180                 185                 190         


Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro 
        195                 200                 205             


Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
    210                 215                 220                 


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
225                 230                 235                 240 


Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 
                245                 250                 255     


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 
            260                 265                 270         


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
        275                 280                 285             


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 
    290                 295                 300                 


Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
305                 310                 315                 320 


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys 
                325                 330                 335     


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
            340                 345                 350         


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
        355                 360                 365             


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
    370                 375                 380                 


Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 
385                 390                 395                 400 


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
                405                 410                 415     


Leu Ser Leu Ser Pro Gly Lys Lys 
            420                 


